CN104095997A - Application of Pu'er tea extract in preparation of drug or food for protecting gastric mucosa injure - Google Patents
Application of Pu'er tea extract in preparation of drug or food for protecting gastric mucosa injure Download PDFInfo
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Abstract
The invention relates to application of Pu'er tea extract in preparation of a drug or health care food for protecting gastric mucosa injure stomach trouble, the gastric mucosa injure stomach trouble includes gastritis, gastric ulcer, gastric bleeding, erosion, hyperemia, edema and the like. The preparation of the Pu erh tea extract comprises the following steps: step 1, adding water into Pu'er tea for decocting, concentrating to a certain proportion; and step 2, centrifuging the concentrated liquid, concentrating the centrifugate, and drying to obtain the Pu erh tea extract.
Description
Technical field
The present invention relates to the application of a kind of Pu'er tea in medicine or the food of preparation treatment mucosal lesion gastropathy, belong to pharmacy and field of health care food.
Background technology
Folium camelliae assamicae original producton location is mainly in Simao Diqu and Xishuangbanna in Yunnan.Along with the popularization of large leaf, Sichuan, Guangdong, Guangxi also become the province that Folium camelliae assamicae is produced.Along with expanding economy, the enhancing of people's health care consciousness, Folium camelliae assamicae is sweet because of mellow time of its flavour, and the fine quality of CHENXIANG uniqueness and its special health-care effect to human body play concern and the attention of the whole society, the favor of product Shen Shou consumer.
Warm in nature and the storage tolerance of Folium camelliae assamicae, be suitable for cooking with or bubble drink.The many historical book of Ancient Times in China has much records about Folium camelliae assamicae effect: ZHAO Xue-Min supplementary Amplifications of the Compendium of Materia Medica is recorded, and Pu Hei is as paint, and relieving alcoholic intoxication the first, eliminates indigestion and phlegm, and clearing stomach is promoted the production of body fluid, and power especially greatly also; Cloud again in " woody part ", Pu'er tea paste can be controlled all kinds of diseases and ailments, as tripe catches cold, disperses with ginger decoction, and perspiration can be healed, and mouthful broken larynx is dry, and the pain of being heated, chews and heals with five points the night of making a slip of the tongue; Chen Zonghai writes, and " Pu'er is helped digest and is dispersed cold, has Detoxication in " interview of the Room, Simao " record." clear scholar's Song hero " occupying diet spectrum with breath " cloud " Pu'er product person, the highly seasoned kind wind-phlegm meat that disappears of telling, all filthy pathogen in summer string-shaped mass in the abdomen stomachache, the diseases such as cholera dysentery are often healed from the beginning of, drink ".Forefathers think to Pu'er tea health-care experience as can be seen here, and Folium camelliae assamicae has and helps digestion except poison, regulate the flow of vital energy swollen, and removing heat-phlegm, wind dispelling relieving alcoholic intoxication, controls the effects such as dysentery is antibacterial.
Current research thinks that Folium camelliae assamicae has the health-care effect of several respects:
1, lowering blood-fat and reducing weight: Folium camelliae assamicae and fatty metabolism are in close relations, Folium camelliae assamicae has generated new chemical substance through unique sweat, the lipase that contains lipase wherein having, can produce decomposition to fat, thereby Folium camelliae assamicae has the effect of fat-reducing.
2, blood pressure lowering, arteriosclerosis.
3, anti-cancer, anticancer: scientist, by a large amount of crowd's comparisons, proves that the crowd's of drinking tea cancer morbidity is lower.And Folium camelliae assamicae contains multiple abundant anticancer trace element, the tumoricidal effect of Folium camelliae assamicae is strong.
4, strong protecting teeth: contain many physiologically active ingredients in Folium camelliae assamicae, there is the effect of sterilizing, therefore can remove halitosis, take care of one's teeth.
5, protect stomach, nourishing the stomach: under suitable concentration; drink gentle Folium camelliae assamicae the intestines and stomach is not produced to stimulation; thickness, sweet cunning, mellow Folium camelliae assamicae enter the film that human body the intestines and stomach forms and adhere to the top layer of stomach; stomach is produced to useful protective layer, long-term can play the effect of protecting stomach, nourishing the stomach after drinking Folium camelliae assamicae.This is to advocate the main cause that consumer's appellation Folium camelliae assamicae of drinking Folium camelliae assamicae is " cosmetic tea ", " tea lengthens one's life " both at home and abroad.
6, defying age: prove after deliberation, in human body, lipid peroxidation process is one of mechanism of human senility.Vitamin C, vitamin E, tea polyphenols, aminoacid and the trace element etc. that in Folium camelliae assamicae, contain have antioxidation, delaying senility course, and therefore Folium camelliae assamicae is called as " tea lengthens one's life ".
7, radioprotective: show by the result of study that Folium camelliae assamicae carries out according to what state's fence of Guangdong Zhongshan University etc., drink 2% Folium camelliae assamicae and can remove the injury causing with co-60 radiation.
8, relieving alcoholic intoxication: supplementary Amplifications of the Compendium of Materia Medica carries: " general tea is controlled oil most and covered pericardium, postcibal diarrhea, relieving alcoholic intoxication first." medical evidence: the tea polyphenols in Folium Camelliae sinensis can promote alcohol metabolism, and liver is had to protective effect.Alcohol metabolism can normally be carried out smoothly.Have tea and can increase vasoconstriction function.Theophylline has diuresis, can impel ethanol to excrete fast, reduces the harm after drunk.Drink tea and can also supplement ethanol and be hydrolyzed required vitamin C, excited large mesencephalic centre of being anaesthetized by ethanol.Thereby play antialcoholism action.
The lipid-reducing function of Folium camelliae assamicae receives wide acceptance at present.But the effect for the treatment of mucosal lesion gastropathy never has direct experimental data support.
Because current consumption habit just brews and drinks with Folium Camelliae sinensis, functional component brewed number of times, brew temperature, brewing time etc. affects and causes stripping difference, can not utilize completely.
Summary of the invention
The invention provides medical treatment and health purpose that a kind of Pu'er tea is new.
The present invention also provide contain pharmaceutical composition of extract of the present invention and preparation method thereof with and medicine or the health food of preparation treatment mucosal lesion gastropathy in purposes.
Pu'er tea of the present invention, obtains through extracting processing, its extracting method, and step is as follows:
Step 1, puerh tea leaves decocts with water, and extracting liquid filtering is concentrated to certain weight proportion;
Step 2, concentrated solution is centrifugal, and centrifugal liquid is concentrated, is drying to obtain.
Preferably, extracting method of the present invention, step is as follows:
Step 1, puerh tea leaves decocts with water and extracts 2-4 time, and each 0.5～2 hour, the water of 6-12 times of volume; Extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2-1:3,
Step 2, concentrated solution is centrifugal with centrifuge, centrifugal liquid is evaporated to proportion 1.1-1.25(45-65 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).
It is preferred,
Extracting method of the present invention, step is as follows:
Step 1, puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 times, and each 0.5～2 hour, the water of 6-12 times of volume; Preferably (1.5h, 1.5h, 1h; 10 times of volumes, 8 times of volumes, 8 times of volumes).Extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2-1:3,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, tripodia centrifugal liquid is centrifugal with tube centrifuge, centrifugal liquid is evaporated to proportion 1.1-1.25(45-65 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).
Wherein tubular type centrifugal condition: turn/min of centrifuge speed: 15000-19000; Spraying drying condition: inlet temperature: 140-190 DEG C, leaving air temp: 75-95 DEG C.
Pu'er tea of the present invention includes but not limited to above extract, and also comprising goes on the market can purchase from market available Folium camelliae assamicae goods, as Folium camelliae assamicae treasure, and Pu'er beverage, the medicine and the food etc. that contain Pu'er tea.Pu'er tea of the present invention also comprises Pu'er tea prepared by any art methods, as Folium camelliae assamicae extracting method and extract thereof in Chinese patent application prospectus, these extracts have same or analogous effect because all contain the functional components extracting from Folium camelliae assamicae.
The preferred Pu'er tea of the present invention is Folium camelliae assamicae treasure, is a kind of Pu'er tea goods of list marketing, after can brewing, takes, and its methods for making and using same all belongs to prior art.
The extract of the present invention obtaining through above method can be used as the active fraction preparation with treatment gastropathy function and becomes pharmaceutical composition or health food.Health food of the present invention includes but not limited to: beverage, milk product, Folium Camelliae sinensis, cake etc.
Mucosal lesion gastropathy of the present invention includes but not limited to: gastritis, gastric ulcer, gastrorrhagia, erosion, hyperemia, edema etc.
Active constituents of medicine in pharmaceutical composition of the present invention, its shared percentage by weight in compositions can be 0.1-99.9%, all the other are medicine acceptable carrier.Pharmaceutical composition of the present invention, exists with unit dosage form, and described unit dosage form refers to the unit of preparation, as every of tablet, and every capsules of capsule, every bottle of oral liquid, every bag of granule etc.
Pharmaceutical composition of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, preferably peroral dosage form, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum etc.
Pharmaceutical composition of the present invention, the preparation of its oral administration can contain conventional excipient, such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet if desired.
Applicable filler comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, for example sodium starch glycollate.Suitable lubricant comprises, for example magnesium stearate.The acceptable wetting agent of suitable medicine comprises sodium lauryl sulphate.
Can, by mixing, fill, the method that tabletting etc. are conventional is prepared solid oral composition.Repeatedly mix and can make active substance be distributed in those compositionss of a large amount of filleies of whole use.
The form of oral liquid can be for example aqueous or oily suspensions, solution, Emulsion, syrup or elixir, or can be a kind of available water before use or the composite dry products of other suitable carrier.This liquid preparation can contain conventional additive, such as suspending agent, for example sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenation edible fat, emulsifying agent, for example lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), for example almond oil, fractionated coconut oil, such as oily ester, propylene glycol or the ethanol of the ester of glycerol; Antiseptic, for example para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if need, can contain conventional flavouring agent or coloring agent.
For injection, the liquid unit dosage forms of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, this compound can be suspended or dissolve.The preparation of solution, normally by active substance being dissolved in a kind of carrier, being packed into filter-sterilized before a kind of suitable bottle or ampoule, then seals.Adjuvant for example a kind of local anesthetic, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, can be by freezing this compositions after packing bottle into, and under vacuum, water is removed.
Pharmaceutical composition of the present invention, in the time being prepared into medicament, optionally add applicable medicine acceptable carrier, described medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and derivant thereof, alginate, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Compositions of the present invention is determined usage and dosage according to patient's situation in use, can take every day three times, each 1-20 agent, as: 1-20 bag or grain or sheet.
The present invention also comprises the application of Pu'er tea of the present invention in the medicine of preparation treatment mucosal lesion gastropathy.
Pu'er tea of the present invention has good health care and treatment function, particularly aspect treatment mucosal lesion gastropathy.
Below data declaration beneficial effect of the present invention by experiment:
Folium camelliae assamicae treasure causes the protective effect research of acute gastric mucosal injury to dehydrated alcohol
The particular content of experiment is as follows:
1. experiment purpose
This research adopts dehydrated alcohol to cause gastric mucosa damage model and evaluates the impact of Folium camelliae assamicae treasure on mucosal lesion.
Acute gastric mucosal injury is a clinical common class digestive system disease, how in the time of body severe trauma, shock, infection, to occur, taking erosion, the ulcer, hemorrhage as principal character of gastric mucosa.This experiment adopts the animal model of alcohol induced acute gastric mucosal injury, and ethanol is acknowledged as a kind of damage factor in animal and clinical research, can cause that significant mucosa hyperemia, necrosis, edema and mucosa or Submucosa are hemorrhage.The formation of infringement is mediated by oxygen-derived free radicals.
2. materials and methods
2.1 experiment material
Laboratory animal and feeding environment: 80 of SPF level SD rats, male, body weight 180～200g, is purchased from Beijing dimension tonneau Hua Shi animal Technology Co., Ltd..In whole experimentation, animal freely ingests and drinks water, 18～22 DEG C of room temperatures, relative humidity 50%～60%.
Be subject to reagent: Pu'er tea lot number: 2010F01.
Reagent: dehydrated alcohol is analytical pure, is produced lot number: 20110812 by Li Anlongbohua (Tianjin) medical chemistry company limited.
2.2 experimental technique
2.2.1 experiment grouping
80 rats are divided at random: dosage group (1g/kg), Pu'er tea high dose group (1.5g/kg) in Normal group, model group, Pu'er tea low dose group (0.5g/kg), Pu'er tea, 9 every group.The continuous gavage of each tested material gives 14d, tests in modeling in the 15th day.
2.2.2 dehydrated alcohol causes gastric mucosa damage model
Before model preparation, water 24h is can't help in animal fasting, Normal group is with normal saline 1mL gavage, all the other are respectively organized all with dehydrated alcohol 1mL gavage, after 1h, put to death animal (before putting to death, blood sampling is for the detection of biochemical indicator), cut open the belly ligation stomachus pyloricus and pars cardiaca, and inject 10% formalin 10mL to gastric, then get stomach and be placed in 10% formalin and fix 20min.
2.3 observation indexs and method
1. mucosal lesion situation: fixing stomach is cut off along greater gastric curvature, and normal saline washes away gastric residue, launches gastric mucosa, observes and evaluate damage index.With length and the width of vernier caliper measurement petechia or hemorrhage band.Because the seriousness of width representative damage is large far beyond length, therefore double integration.Its standards of grading are in table 1.
Table 1 acute alcohol damage naked eyes are seen and are looked into standards of grading
Evaluation index: each experimental group gastric mucosa injury degree represents with the rate of injury (%), damage integral exponential and damage suppression ratio.There is rat quantity/this group rat quantity × 100% of hemorrhage or ulcer in the rate of injury (%)=certain group; Damage integral exponential=group Injury score summation/treated animal quantity; Damage suppression ratio (%)=(A-B)/A × 100%(A, B is respectively the damage integration of model group and test group).
2. Plasma SOD (SOD), malonaldehyde (MDA) are measured: press test kit description operation.
3. histopathologic examination and scoring: gross examination is complete, cuts every the most serious position of gastric mucosa of animal damage, is fixed on 10% formalin, conventional film-making, HE dyeing, Microscopic observation.Change cutter → dehydration → transparent → waxdip → embedding → section → dyeing → mounting.
Instrument and reagent: holy JQC-A type pathologic sampling worktable of a specified duration; LEICA ASP300 pathological tissue dehydrating machine; LEICAEG1140C pathologic tissue packing machine; LEICA RM2145 pathologic section slicer.Ethanol, the medical company limited of Amway Long Bohua (Tianjin); Formaldehyde, the medical company limited of Amway Long Bohua (Tianjin); Dimethylbenzene, the medical company limited of Amway Long Bohua (Tianjin); Paraffin, Leca company; Haematoxylin dye liquor, Japanese Wu Teng KCC; Eosin stain, the medical company limited of Amway Long Bohua (Tianjin).
Read sheet: adopt OLYMPUS BX53 pathology microscope to read sheet, and typical cytopathic is taken a picture with OLYMPUS DP72 image capturing system.
The positive cross section of attentional selection gastric mucosa, comprises the regional observation of mucosa holostrome.Methods of marking: be divided into 5 grades in the degree of involving of whole mucous epithelium layer with congested, hemorrhage, mucomembranous cell degeneration necrosis.Congested weight is 1, and hemorrhage weight is 2, and epithelial cell degeneration necrosis weight is 3, and standards of grading and pathological changes total mark formula are in table 2.
Standards of grading under table 2 acute gastric mucosal lesion mirror
2.4 statistical method
Adopt SPSS10.0 software analysis, experimental result adopts the relatively significance of group difference of one factor analysis of variance.
3. experimental result
The impact of 3.1 Pu'er teas on mucosal lesion
Result shows, Pu'er tea (0.5g/kg, 1g/kg, 1.5g/kg) gavage is the generation that dose dependent obviously suppresses dehydrated alcohol and cause gastric mucosa damage, and damage suppression ratio is respectively: 17.48%, 45.00%, 72.00%; Wherein high dose group is better than cimetidine group, and prompting Pu'er tea causes gastric mucosa damage to dehydrated alcohol significant protective effect.
Table 3 Folium camelliae assamicae treasure causes the impact of mucosal lesion on dehydrated alcohol
Note: * * and model compare P ﹤ 0.01 ， ﹡ and model compares P ﹤ 0.05
3.2 the impact of Pu'er tea on activity of SOD in serum and MDA content
Research shows that polytype acute gastric mucosal injury is all relevant with oxygen-derived free radicals, and SOD and MDA are all antioxidative indexs in body serum.Prove that the acute gastric mucosal injury of alcohol-induced and the blood flow of oxygen-derived free radicals and gastric mucosa reduce relevant.This research finds that the precious high dose group of Folium camelliae assamicae is improved effect to SOD in serum level, and model group relatively has and significantly improve, and difference has statistical significance (P<0.01), and prompting Folium camelliae assamicae treasure has the effect that improves oxidation resistance.The results are shown in Table 4.
The impact of table 4 Folium camelliae assamicae treasure on activity of SOD in serum and MDA content
Note: * * and model compare P ﹤ 0.01 ， ﹡ and model compares P ﹤ 0.05
The impact of 3.3 Pu'er teas on pathomorphology
Normal group: rarely seen hyperemia.Model group: the necrosis of gastric mucosa 3/5-4/5 epithelial cell comes off, congestion and edema, part has hemorrhage, partial wall cell and chief cell degeneration necrosis, pathological changes area is larger, darker.Cimetidine group, high, normal, basic group of same model group of pathological changes of Folium camelliae assamicae, but pathological changes is marked all lower than model group.The results are shown in lower list 5,6,7,8,9,10 and Fig. 2,3,4,5,6,7.
Table 5 normal group gastric mucosa injury pathological changes appraisal result
Table 6 model group gastric mucosa injury pathological changes appraisal result
Table 7 cimetidine group gastric mucosa injury pathological changes appraisal result
The precious low dose group gastric mucosa injury of table 8 Folium camelliae assamicae pathological changes appraisal result
Dosage group gastric mucosa injury pathological changes appraisal result in table 9 Folium camelliae assamicae treasure
The precious high dose group gastric mucosa injury of table 10 Folium camelliae assamicae pathological changes appraisal result
4. discuss
This research is by adopting dehydrated alcohol to cause gastric mucosa damage model; find to give in advance Pu'er tea gavage; within continuous 14 days, can suppress the caused acute gastric mucosal injury of dehydrated alcohol in dose dependent ground; suppression ratio is followed successively by from low to high: 17.48%; 45.00%; 72.00%, show that Pu'er tea has significant protective effect to acute gastric mucosal injury.Blood biochemical result shows that the precious high dose group of Folium camelliae assamicae can significantly improve SOD content in serum.Pathological examination results shows that the precious each dosage group of Folium camelliae assamicae has protective effect to gastric mucosa.
Advantage of the present invention is: a kind of Pu'er tea is provided, this extract evidence has good treatment mucosal lesion gastropathy function, active constituent content is high simultaneously, steady quality, easy to carry, be convenient to take, can be used for preparing medicine and various health food or health tea, play good treatment gastropathy effect.
Brief description of the drawings
Fig. 1 is each experimental group affects situation specimen photo on mucosal lesion.
Fig. 2 normal group gastric mucosa injury pathological changes mirror hypograph (400 times)
Fig. 3 model group gastric mucosa injury pathological changes mirror hypograph (400 times)
Fig. 4 cimetidine group gastric mucosa injury pathological changes mirror hypograph (400 times)
The precious low dose group gastric mucosa injury pathological changes mirror hypograph of Fig. 5 Folium camelliae assamicae (400 times)
Dosage group gastric mucosa injury pathological changes mirror hypograph (400 times) in Fig. 6 Folium camelliae assamicae treasure
The precious high dose group gastric mucosa injury pathological changes mirror hypograph of Fig. 7 Folium camelliae assamicae (400 times)
Detailed description of the invention
Further illustrate by the following examples the present invention, but not as limitation of the present invention, the water extract of Folium camelliae assamicae all has effect of the present invention.
Embodiment 1
Pu'er tea production technology:
Puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 (1.5h, 1.5h, 1h; 10bv, 8bv, 8bv), extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2-1:3, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, tripodia centrifugal liquid is centrifugal with tube centrifuge, centrifugal liquid is evaporated to proportion 1.1-1.25(45-65 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).
Wherein tubular type centrifugal condition: centrifuge speed: 17000 turn/min; Spraying drying condition: inlet temperature: 160 DEG C, leaving air temp: 85 DEG C.
Embodiment 2
Step 1, puerh tea leaves decocts with water and extracts 3 times, and each 0.5 hour, the water of 6 times of volumes; Extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2,
Step 2, concentrated solution is centrifugal with centrifuge, centrifugal liquid is evaporated to proportion 1.1(45 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).
Embodiment 3
Step 1, puerh tea leaves decocts with water and extracts 3 times, and each 2 hours, the water of 12 times of volumes; Extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:3,
Step 2, concentrated solution is centrifugal with centrifuge, centrifugal liquid is evaporated to proportion 1.25(65 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).
Embodiment 4
Step 1, puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 times, and each 0.5 hour, the water of 6 times of volumes; Extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, tripodia centrifugal liquid is centrifugal with tube centrifuge, centrifugal liquid is evaporated to proportion 1.1(45 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).
Wherein tubular type centrifugal condition: centrifuge speed: 15000 turn/min; Spraying drying condition: inlet temperature: 140 DEG C, leaving air temp: 75 DEG C
Embodiment 5
Step 1, puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 times, and each 2 hours, the water of 12 times of volumes.Extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:3,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, tripodia centrifugal liquid is centrifugal with tube centrifuge, centrifugal liquid is evaporated to proportion 1.25(65 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).
Wherein tubular type centrifugal condition: centrifuge speed: 19000 turn/min; Spraying drying condition: inlet temperature: 190 DEG C, leaving air temp: 95 DEG C
Embodiment 6
Puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 (1h, 1h, 1h; 6bv, 6bv, 6bv), extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2.5, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, tripodia centrifugal liquid is centrifugal with tube centrifuge, centrifugal liquid is evaporated to proportion 1.1-1.25(50 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turn/min; Spraying drying condition: inlet temperature: 160 DEG C, leaving air temp: 85 DEG C
Embodiment 7
Puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 (2h, 2h, 2h; 10bv, 10bv, 10bv), extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2.5, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, tripodia centrifugal liquid is centrifugal with tube centrifuge, centrifugal liquid is evaporated to proportion 1.1-1.25(50 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turn/min; Spraying drying condition: inlet temperature: 160 DEG C, leaving air temp: 85 DEG C
Embodiment 8
Puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 2 (0.5h, 0.5h; 12bv, 12bv), extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2.5, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to proportion 1.1-1.25(50 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turn/min; Spraying drying condition: inlet temperature: 160 DEG C, leaving air temp: 85 DEG C
Embodiment 9
Puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 4 (2h, 2h, 2h, 2h; 10bv, 10bv, 10bv, 10bv), extracting liquid filtering, filtrate decompression concentrates (≤70 DEG C) to Folium Camelliae sinensis (weight): concentrated solution (volume)=1:2.5, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to proportion 1.1-1.25(50 DEG C), condensed cream spraying is drying to obtain (or microwave drying, pulverize and get final product).Wherein tubular type centrifugal condition: centrifuge speed: 17000 turn/min; Spraying drying condition: inlet temperature: 160 DEG C, leaving air temp: 85 DEG C
Embodiment 10
According to any one extract in embodiment 1-9, add conventional adjuvant, the conventional method on application galenic pharmacy, makes tablet.
Embodiment 11
According to any one extract in embodiment 1-9, add conventional adjuvant, the conventional method on application galenic pharmacy, makes capsule.
Embodiment 12
According to any one extract in embodiment 1-9, add conventional adjuvant, the conventional method on application galenic pharmacy, granulation agent.
Embodiment 13
According to any one extract in embodiment 1-9, add conventional adjuvant, the conventional method on application galenic pharmacy, makes powder.
Embodiment 14
According to any one extract in embodiment 1-9, add conventional adjuvant, the conventional method on application galenic pharmacy, makes drop pill.
Embodiment 15
According to any one extract in embodiment 1-9, add conventional adjuvant, the conventional method on application galenic pharmacy, makes injection.
Embodiment 16
According to any one extract in embodiment 1-9, join by a certain percentage in tea product with common process, make the health tea with protection gastric mucosa function.
Embodiment 17
According to any one extract in embodiment 1-9, join by a certain percentage in milk product with common process, make the health food with protection gastric mucosa function.
Embodiment 18
According to any one extract in embodiment 1-9, join by a certain percentage in beverage with common process, make the health beverage with protection gastric mucosa function.
Embodiment 19
According to any one extract in embodiment 1-9, join by a certain percentage in cake with common process, make the health care cake with protection gastric mucosa function.
Claims (10)
1. a Pu'er tea is in the application of preparing in medicine or the health food for the treatment of mucosal lesion gastropathy.
2. according to the application of claim 1, it is characterized in that, described mucosal lesion gastropathy comprises: gastritis, gastric ulcer, gastrorrhagia, erosion, hyperemia, edema etc.
3. according to the application of claim 1, it is characterized in that, described Pu'er tea is Folium camelliae assamicae water solubility extract.
4. according to the application of claim 1, it is characterized in that, described Pu'er tea, extraction step is as follows:
Step 1, puerh tea leaves decocts with water and extracts 2-4 time, and each 0.5～2 hour, the water of 6-12 times of volume; Extracting liquid filtering, filtrate is evaporated to Folium Camelliae sinensis weight under≤70 DEG C of conditions: concentrated solution volume=1:2-1:3,
Step 2, concentrated solution is centrifugal with centrifuge, and centrifugal liquid is evaporated to 45-65 DEG C of proportion 1.1-1.25, and condensed cream spraying is dried or microwave drying, to obtain final product.
5. according to the application of claim 1, it is characterized in that, described Pu'er tea, extraction step is as follows:
Step 1, puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 times, and each 0.5～2 hour, the water of amount of water 6-12 times volume; Extracting liquid filtering, filtrate is evaporated to Folium Camelliae sinensis weight under≤70 DEG C of conditions: concentrated solution volume=1:2-1:3,
Step 2, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to 45-65 DEG C of proportion 1.1-1.25, and condensed cream spraying is dried or microwave drying, to obtain final product;
Wherein tubular type centrifugal condition: turn/min of centrifuge speed: 15000-19000; Spraying drying condition: inlet temperature: 140-190 DEG C, leaving air temp: 75-95 DEG C.
6. according to the application of claim 1, it is characterized in that, described Pu'er tea, extraction step is as follows:
Puerh tea leaves adds water acutely to seethe with excitement to decoct and extracts 3 times, decocts 1.5h the 1st time, and 10 times of volumes add water; Decoct 1.5h the 2nd time, 8 times of volumes add water; Decoct 1h the 3rd time, 8 times of volumes add water, extracting liquid filtering, filtrate is evaporated to Folium Camelliae sinensis weight under≤70 DEG C of conditions: concentrated solution volume=1:2-1:3, concentrated solution is centrifugal with tripod pendulum type batch centrifugal, and tripodia centrifugal liquid is centrifugal with tube centrifuge, and centrifugal liquid is evaporated to 45-65 DEG C of proportion 1.1-1.25, condensed cream spraying is dried or microwave drying, to obtain final product;
Wherein tubular type centrifugal condition: turn/min of centrifuge speed: 15000-19000; Spraying drying condition: inlet temperature: 140-190 DEG C, leaving air temp: 75-95 DEG C.
7. according to the application of claim 1, it is characterized in that, described application comprises takes the pharmaceutical composition of Pu'er tea as active constituents of medicine.
8. according to the application of claim 7, it is characterized in that described pharmaceutical composition is any pharmaceutically useful dosage form.
9. application according to Claim 8, it is characterized in that, described medicine composition dosage form is selected from: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop or patch.
10. according to the application of claim 1, it is characterized in that, described health food is selected from: beverage, milk product, Folium Camelliae sinensis or cake.
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| CN105797199A (en) * | 2016-04-07 | 2016-07-27 | 杨兰 | Band-aid containing pu'er tea extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102727663A (en) * | 2011-04-14 | 2012-10-17 | 云南天士力帝泊洱生物茶集团有限公司 | Application of Pu'er tea extract in preparing hypotensive medicine or foodstuff |
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| CN102727663A (en) * | 2011-04-14 | 2012-10-17 | 云南天士力帝泊洱生物茶集团有限公司 | Application of Pu'er tea extract in preparing hypotensive medicine or foodstuff |
Non-Patent Citations (2)
| Title |
|---|
| 张建凡: "喝普洱茶可养胃护胃", 《家庭科学》 * |
| 段振离编: "《红楼说茶：《红楼梦》中的茶文化与养生》", 30 April 2011, 上海交通大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105797199A (en) * | 2016-04-07 | 2016-07-27 | 杨兰 | Band-aid containing pu'er tea extract |
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