KR101359806B1 - Helianthus tuberous. and Cudrania tricuspidata complex extracts for decreasing blood glucose level and health funtional food comprising the same - Google Patents

Abstract

The present invention relates to a complex extract of pork potato and courageous leaf extract, which is effective in lowering blood sugar, and the complex extract of the present invention has an amount of 3/10 to 7/10 compared to the respective contents of the pork potato and jacuzzi leaf. Even if it contains, the synergistic effect is excellent in hypoglycemic effect can not only be developed as a diabetes treatment, but also can be used in health functional food for improving blood sugar of diabetes-related diseases.

Description

Combined extract of pork potato and Cudrania leaf efficacious in reducing blood sugar and health functional food composition using the same {Helianthus tuberous. and Cudrania tricuspidata complex extracts for decreasing blood glucose level and health funtional food comprising the same}

The present invention is a pig potato ( Helianthus) effective in lowering blood sugar tuberous ) and Cudrania leaves tricuspidata ) relates to a complex extract and a dietary supplement composition using the same.

With the advent of westernized eating habits, lifestyle changes, and aging societies, various adult diseases such as cancer, cerebrovascular disease, and heart disease are increasing. Among them, the prevalence of diabetes is steadily increasing, and high mortality as well as acute and chronic complications threaten national health. Diabetes is a type of metabolic disease in which insulin secretion is insufficient or does not function normally. Hyperglycemia occurs due to high glucose levels in the blood, and glucose is released into the urine. Insulin deficiency is caused by the destruction of the pancreatic β-cells. It can be divided into type 1 diabetes caused by type 2 diabetes caused by defects in insulin secretion and action. In this diabetes, pancreatic β-cells secrete insulin to regulate blood sugar, insulin receptors are present and play an important role in insulin signaling. Recently, the growth and proliferation of β-cells affect the treatment and prevention of diabetes. Studies have been reported.

In Korea, insulin-independent type 2 diabetic patients account for 90-95% of all diabetic patients, and most of them control blood sugar by pharmacotherapy, but more and more side effects are required as time passes. Therefore, in order to prevent this, the importance of the discovery of active substances with diabetes improvement function and product development is emphasized.

Pork Potato ( Helianthus tuberous ) is a perennial plant of the Asteraceae, also called "fatty", sometimes referring to its root, caliber. Country of origin is eastern Canada and northeastern United States. The end of the underground stem is thickened and tubers develop. Stems stand upright, branches are split, height is 1.5 ~ 3m, and there are centriles. The leaves face at the bottom of the stem, are displaced at the top, have a long oval shape, pointed ends, serrated at the edges, and narrow at the bottom to flow into the petioles, becoming wings. Tubers vary in shape from elongated to rugged and vary in size and weight. Tuber shell color also varies from light yellow to brown to red to purple. The skin is so thin that when exposed to dry air, it quickly creases and crusts. Tubers are cultivated for food use, but now they grow wild near permits, and some are planted for livestock feed. In oriental medicine, the root is used as a medicine called gukwoo (菊芋), fever is an action that stops bleeding mass.

In recent years, various studies have been carried out with the fact that the pork stem has an excellent effect on the treatment of diabetes and prevention of complications, containing about 15% or more of inulin called natural insulin. First, Korean Unexamined Patent Publication No. 2010-33064 discloses a composition for preventing or improving obesity or type 2 diabetes, including a pig potato extract as an active ingredient. In addition, Korean Patent Laid-Open Publication Nos. 2006-41438, 2003-26384, and 2012-14744 include the addition of Schisandra potato, jujube, ginseng, and upper leaf powder or extract, showing the effect on diabetes. It is described. However, the hypoglycemic effect shown in these documents does not show a relatively excellent effect. Therefore, a variety of studies are still underway using pig potatoes.

Cudrania tricuspidata belonging to the deciduous broad-leaved subfamily of Dicotyledonous nettle, Mulberry family, is also known as Goodthorn Tree. It grows in the foothills and near the village. The branches have thorns, and the leaves are divided into three branches, and the edges are flat and egg-shaped. The three-branched leaf is dull at the end and rounded at the bottom. The egg-shaped leaves are sharp at the ends and bottom, 6-10cm long, 3-6cm wide, with fine hairs on the surface, and villi on the back are entangled with each other. Petioles are 15-25mm long, with hairs. The leaves are used as a mulberry leaf substitute, the fruit can be eaten, jam or soaked, and the bark and roots are used as medicinal or paper ingredients.

Root bark, tree bark, bark and leaves of Cudrania japonica contain various ingredients effective for the human body. From now on, Cudrania japonica is a blood pressure lowering agent, tuberculosis depending on the root, bark, stem, leaf, bark, fruit, etc. It has been used as a medicine for antipyretics, antipyretics, expectorants, diuretics, hemostatics, and antiperspirants. It is used for athlete's foot as an antifungal agent and for chronic dyspepsia due to weakness of digestive organs. In addition, stem bark has been reported to contain a large amount of ingredients effective in lung cancer, colon cancer, skin cancer, uterine cancer. Cudrania japonica is known as anti-cancer effect, because it contains a flanovoid known as an anticancer effect, synonymous bogam, weakness, herbal wood, etc. from ancient times.

Recently, various studies using extracts from courageous tree have been continuously conducted, and in the paper J East Asian Soc Dietary Life, 18 (4): 516 ~ 523 (2008), the anti-diabetic effect of tea and fermented tea made from coupe leaf Korean Patent Laid-Open Publication No. 2012-26992 discloses an effect of inhibiting obesity and diabetes, including kkujippong extract and Euiyiin extract. In addition, the Republic of Korea Patent No. 10-815212 is provided with a combination of kkujippong leaf extract and indigestible maltodextrin that can control blood sugar and weight. However, the hypoglycemic effect shown in these documents shows a relatively insignificant effect. Therefore, a variety of researches are still underway using coujippong.

The inventors of the present invention while studying a health functional food composition that can improve the blood sugar of diabetics, even if the extract of pig potato and kkujippong contains about half each of the content shows a significant effect on the blood sugar drop by synergism It confirmed and completed this invention.

The present invention is to provide a composite extract of swine potato and cooji mulberry leaves effective in lowering blood sugar and health functional food composition using the same.

In order to achieve the above object, the present invention provides a composite extract of dried pork potato and kkujippong leaves.

In addition, it provides a health functional food composition containing a complex extract according to the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention is to prepare a composite extract by mixing and extracting dried pork potato and kkujippong leaves. In the present invention, the contents of the dried pork potato and Cudrania leaf used to prepare the complex extract contain an amount of 3/10 to 7/10 relative to the respective content, and the content ratio is 3: 7 to 7: 3 weight %, The most preferred content of the composite extract is to prepare the pork potato and kkujippong leaves containing 4: 6 to 6: 4% by weight.

Extraction method according to the invention can be prepared using conventional extraction methods prepared when using herbal medicines and natural products. The extraction method is preferably any one selected from the group consisting of hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction and steam extraction, but is not limited thereto. Preferred extraction method is hot water extraction method.

The present invention is 3: 7 to 7: 3% by weight of pig potatoes and Cudrania leaves, preferably the content of the composite extract contains 4: 6 to 6: 4% by weight of pig potatoes and Cudrania leaves, pharmaceutically acceptable Possible carriers, diluents or excipients may be formulated into preparations for the prevention and treatment of diabetes.

The herbal extract required for formulation according to the present invention comprises 0.01 to 80%, preferably 1 to 50% by weight of the extract relative to the total weight of the composition.

Carriers, excipients and diluents that may be included in the compositions of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

In addition, the compositions of the present invention may be used in the form of oral dosage forms, external preparations, suppositories, or sterile injectable solutions, such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, respectively, according to conventional methods. Can be.

More specifically, when formulating the composition, it can be prepared using a diluent or an excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, and the like. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate, sucrose, lactose, gelatin, etc. Can be prepared by mixing. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, syrups and the like, and various excipients such as wetting agents, sweeteners, fragrances, preservatives, etc. in addition to commonly used diluents such as water and liquid paraffin . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The complex extract of the present invention may vary depending on the age, sex, and weight of the patient, but in general, an amount of 0.01 to 10 g / kg, preferably 1 to 5 g / kg, may be administered once or several times a day. have. In addition, the dosage may be increased or decreased depending on the route of administration, degree of disease, sex, weight, age, health condition, diet, administration time, administration method, excretion rate, and the like. Thus, the dosage amounts are not intended to limit the scope of the invention in any manner.

In addition, the health functional food for diabetic patients containing the complex extract according to the present invention contains the prepared complex extract as a main ingredient, and may include food additives or excipients that are acceptable in addition to the above components. At this time, food additives and excipients which are acceptable in the food industry include vitamin B1, vitamin B2, vitamin C, vitamin B6, nicotinamide, citric acid, liquid fructose, stevioside, maltodextrin, manuka honey, apple concentrate, lacchurose powder, Sodium silicate, sucrose fatty acid ester, hydroxypropyl methyl cellulose, glycerin fatty acid ester, zinc oxide, beta-cyclodextrin and the like.

The complex extract of the present invention can be prepared in various forms of health functional food by a commonly known method, for example, liquid (beverage, pouch), tablets, pills, hard capsules, soft capsules, granules, etc. Can be.

The complex extract of pork potato and jjippong leaf according to the present invention has excellent efficacy in lowering blood sugar, and can be used as a health functional food composition for improving blood sugar of diabetics as well as being developed as a diabetes treatment.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the present invention is not limited to the following Examples and Experimental Examples.

< Example  1> Pork Potato and Cedar leaf  Preparation of Complex Extract (5: 5 wt%)

After washing 2kg of pork potatoes with purified water, thinly sliced into 10 ~ 15mm using a washing machine and dried at 60 ℃ for about 1 hour. Separately kkujippong 2kg was washed with purified water and dried at 60 ℃ for about 1 hour. 500g of dried pork potato and 500g of Cudrania leaves (5: 5% by weight of main ingredient) were added with 8 times the amount of purified water, extracted twice at 95 ° C for 5 hours, filtered and concentrated to 40 Brix. To prepare a pig and potato kkujippong complex extract.

< Example  2> Pork Potato and Cedar leaf  Preparation of Complex Extract (3: 7 wt%)

The same method as in Example 1 was prepared by combining 300 g of dried pork potatoes and 700 g of Cudrania leaf (compare the main component weight = 3: 7) in the same manner as in Example 1, thereby preparing a complex extract of Porcine Potato and Cudrania leaf.

< Example  3> Pork Potato and Cedar leaf  Preparation of Complex Extract (4: 6 wt%)

In the same manner as in Example 1, 400 g of dried pork potato and 600 g of Cudrania leaf (compared to the main component weight = 4: 6) were prepared in the same manner as in Example 1, thereby preparing a composite extract of Porcine Potato and Cudrania leaf.

< Example  4> Pork Potato and Cedar leaf  Preparation of Complex Extract (6: 4 wt%)

In the same manner as in Example 1, the dried pork potato 600g and 400g Cudrania leaf 400g (compared to the main component weight = 6: 4) was prepared in the same manner as in Example 1 to prepare a pig and Cudrania leaf composite extract.

< Example  5> Pork Potato and Cedar leaf  Preparation of Complex Extract (7: 3 wt%)

In the same manner as in Example 1 700g dried pork potato and 300g kkujippong leaf 300g (compared to the main component weight = 7: 3) was prepared in the same manner as in Example 1 to prepare a pig extract and kkujippong leaves complex extract.

< Comparative Example  1> Preparation of Pork Potato Extract

After washing 2kg of pork potatoes with purified water, thinly sliced into 10 ~ 15mm using a washing machine and dried for about 1 hour in a dryer having a temperature of 50 ~ 60 ℃. 5 kg purified water was added to 1 kg of dried pork potatoes, extracted twice at 95 ° C. for 4 to 5 hours, filtered and concentrated to 40 Brix to prepare pork extract.

< Comparative Example  2> Cedar leaf  Preparation of extract

After washing with 2kg of kkujippong leaves with purified water it was dried for about 1 hour in a dryer having a temperature of 50 ~ 60 ℃. 5 kg of purified water was added to 1 kg of dried Cudrania leaf, and extracted twice at 3 ° C. for 3-4 hours at 95 ° C., followed by filtration and concentrated to 40 Brix to prepare Cudrania leaf extract.

Table 1 shows the main component content ratios of the pork potato extract and Cudrania leaf extract prepared by Examples 1 to 5 and Comparative Examples 1 and 2 of the pork potato and Cudrania leaf composite extract prepared by the present invention.

The blood glucose lowering effect was confirmed through the following experimental examples of Comparative Examples 1 and Comparative Example 2 of the Pork Potato and Cudrania leaf complex extract prepared according to the present invention, Examples 1 to 5 and the Porcine Potato Extract extract. .

< Experimental Example  1> Hypoglycemic effect test

1. Experimental animals

The experimental animals were adapted to the environment by breeding male Sparague-Dawley rats, about 4 weeks old, as pellets for 7 days. After 6 weeks of stabilization for 1 week (weight: 150-170g), the experimental animals were divided into 9 groups with 1 group by the randomized complete block design. Diabetic isolates. That is, the normal group free of distilled water without causing diabetes, the STZ-control group free of negative distilled water after inducing diabetes with STZ, the group of pig potato extracts administered to diabetic rats (HT group, Comparative Example 1 ), HCT-1 group free of negatively mixed pig potato and Cudrania leaf complex extracts mixed with the main components in the diabetic rats (C group, Comparative Example 2) administration group, diabetic rats. 7, Example 2), HCT-2 group (4: 6, Example 3), HCT-3 group (5: 5, Example 1), HCT-4 group (6: 4, Example 4), HCT -5 groups (7: 3, Example 5) were divided and bred.

2. Diabetes Induction by Streptozotocin (STZ)

After fasting the experimental animals for 16 hours to induce diabetes, STZ (Sigma Chemical Co.) (Wright & Lacy 1988), which is known to specifically act on only the β-cells of the pancreas and does not affect other organs, has a pH of 0.01. It was dissolved in 45 mg / kg BW concentration in citrate buffer and injected into the tail vein. Normal group was injected with the same amount of 0.01M citrate buffer solution. About 7 hours after the injection of STZ, blood glucose level rapidly decreases, insulin concentration rises, then blood glucose level rises, insulin concentration decreases due to the destruction of β-cells, and hyperglycemic state persists after 48 hours. After 48 hours after injection, blood was collected from the tail vein and the blood glucose level was measured using a blood glucose meter to determine that the blood glucose concentration was 300 mg / ㎗ or more, and it was used in the experiment.

3. Experimental diet

The dietary intake was measured at a certain time every day to measure the amount of diet consumed per day, and the average daily intake per week was obtained on a weekly basis, and the weight was measured on an animal scale at a regular time once a week. The feed efficiency ratio (FER) was obtained by dividing weight gain by dietary intake.

4. Blood glucose measurement

Blood glucose concentrations of the glucose haelaek chehyeol from a vein in US Pre-cision Q · I · D ® Blood Glucose Monitoring System embodiment (Medisense Inc. USA) by direct measurement of each of Examples 1 to 5 and Comparative Examples 1 to 2 The dropping effect is shown in Table 2 below.

As can be seen in Table 2, all experimental groups containing pig potato and / or kkujippong leaf extract showed a higher blood sugar level than the normal group. There was no significant difference between the STZ-control group and the experimental group (extract) administered up to 3 weeks, but after 4 weeks, the STZ-contro + HT and STZ-control groups were compared with the STZ-control group. In the + CT group, HCT-1 to 5 groups, it was confirmed that the blood glucose level was reduced.

However, in the single extract administration group (STZ-contro + HT group, STZ-control + CT group) prepared in Comparative Example 1 and Comparative Example 2 containing porcine potato or Cudrania leaf extract alone, it was confirmed that the blood glucose level was decreased. It can be seen that the effect is not significantly reduced compared to the -control group.

On the other hand, in the case of the porcine potato and kkujippong leaf complex extract prepared in Examples 1 to 5 of the present invention, the mixture by reducing the amount of 3/10 to 7/10 parts by weight than the pig potato or kkujippong leaf alone extract, respectively Despite being administered in the same amount as the amount of potato or Cudrania leaf extract, the STZ-control group and the single extract administration group prepared in Comparative Examples 1 and 2 (STZ-contro + HT group, STZ-control + CT group) It can be seen that it is significantly reduced in comparison with. In particular, the main components of the pork potato and kkujippong leaves prepared in Examples 1 and 3 to 4 of the present invention by mixing 2/50 to 3/5 parts by weight of the reduced amount of each compared to the pig potato or kkujippong leaves alone extract 4 In the HCT-2 to 4 groups administered the complex extract corresponding to: 6 to 6: 4, there was a significant hypoglycemic effect.

Therefore, the present invention exhibits an excellent effect on the hypoglycemic effect of the complex extract having a complex ratio of 4: 6 to 6: 4 on the basis of the weight (%) of the pork potato and cooji mulberry leaves compared to the respective extracts of pig potato or coo zippong leaves Not only can be developed, it can be usefully used as a dietary supplement composition of diabetes-related diseases.

The following preparation examples show examples of various formulations containing the complex extract of pork potato and cooji mulberry leaves of the present invention, the following preparation examples are merely to illustrate the present invention, thereby not limiting the contents of the present invention .

Preparation Example 1 Preparation of Powder

100 mg of the composite extract of Example 1

Corn Starch 100mg

Lactose 100mg

Talc 10mg

The above ingredients are mixed and filled in airtight cloth to prepare a powder.

&Lt; Formulation Example 2 > Preparation of tablet

100 mg of the composite extract of Example 1

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

&Lt; Formulation Example 3 > Preparation of capsules

100 mg of the composite extract of Example 1

Lactose 50mg

1 mg magnesium stearate

After mixing the above components is compressed into a gelatin capsule prepared by the compression method according to the conventional manufacturing method of the capsule.

Preparation Example 4 Preparation of Liquid

100 mg of the composite extract of Example 1

10g per isomerization

10g per book

Lemon incense quantity

Purified water quantity

According to the conventional method for preparing a liquid solution, each component is added to the purified water, dissolved, and lemon flavor is added, and then purified water is added to adjust the total amount to 100 ml, and then filled into a brown bottle to prepare a liquid solution.

Preparation Example 5 Preparation of Health Food

100 mg of the composite extract of Example 1

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

24.8 mg of magnesium chloride

The composition ratio of the above-mentioned vitamin and mineral mixture is composed of a relatively suitable ingredient suitable for health food as a preferred example, but the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method, and then granulated. It can be prepared and used in the manufacture of health food compositions according to conventional methods.

Preparation Example 6 Preparation of Healthy Drinks

100 mg of the composite extract of Example 1

Revitalization extract 1000 mg

Citric acid 1000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to a total of 900 ml

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions. Although the composition ratio is a composition that is relatively suitable for preference beverages as a preferred example, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, use purpose.

As such, the present invention can provide a functional food that is expected to have a hypoglycemic effect by preparing various foods and beverages containing a complex extract of pork potato and cuddle mulberry leaf.

Claims (7)

delete Porcine potato ( Helianthus tuberous ) and Cudrania tricuspidata ( Cudrania tricuspidata ) is a hypoglycemic complex extract mixed in a 3: 7 to 7: 3 weight ratio.
The complex extract according to claim 2, wherein the pork potato and cuddle leaves are mixed in a weight ratio of 4: 6 to 6: 4.
The complex extract according to claim 2, wherein the Pork Potato and Cudrania leaf extract are extracted by any one method selected from the group consisting of hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction and steam extraction.
The complex extract according to claim 4, wherein the Pork Potato and Cudrania leaf extract are extracted by hot water extraction.
Health functional food for improving blood sugar of diabetic patients containing the complex extract according to claim 2.
Health functional beverage for improving blood sugar of diabetic patients containing the complex extract according to claim 2.