KR101077546B1 - A composition having antidiabetes activity consisting of Ligustrum fruit and red-ginseng Cheongkukjang powder - Google Patents

Abstract

The present invention relates to an anti-diabetic active composition comprising 0.5 to 1: 1 of Ligustrum plant fruit powder or extract and red ginseng-containing Cheonggukjang powder, and a pharmaceutical composition for preventing or treating diabetes and a functional food composition containing the same as an active ingredient. The composition according to the present invention can significantly lower blood sugar in a diabetic-induced animal, and has a very excellent effect in the prevention and treatment of diabetes.

Primate, Light Wood, Red Ginseng Cheonggukjang, Diabetes

Description

A composition having antidiabetes activity consisting of Ligustrum fruit and red-ginseng Cheongkukjang powder}

The present invention relates to an anti-diabetic active composition comprising Ligustrum plant fruit powder or extract and red ginseng-containing Cheonggukjang powder. More specifically, Ligustrum plant fruit powder or extract and red ginseng-containing Cheongukjang powder are 0.5 to It relates to an antidiabetic active composition comprising 1: 1, a pharmaceutical composition for preventing or treating diabetes and a functional food composition containing the same as an active ingredient.

The number of diabetics in Korea has increased significantly in the past 10 years, becoming the fourth cause of death. These phenomena are diagnosed as the effects of economic growth, increased nutritional intake, an increase in obesity population due to lack of exercise, and severe stress, drinking and smoking in hypercompetitive societies. In diabetes, abnormal metabolic metabolism of carbohydrates, proteins and lipids occurs due to hormonal metabolism, resulting in increased blood sugar and lipid content in the blood, sugars and proteins combined to create new toxins, and the polyol pathway. Metabolism progresses to cause diabetes complications. In Korea, diabetes mellitus is synthesized but most of the type 2 diabetes is difficult to work due to its resistance. Diabetes has not yet established definite prophylaxis or treatment, and the exact cause of the disease is not known.

Known diabetes prevention and treatment methods to date are known diet and exercise therapy and drug therapy, most often combined with these therapies. Among these therapies, diet and exercise therapy are less effective, and drug therapy has new side effects, and there are numerous studies to discover antidiabetic materials using natural products.

The present inventors have conducted a study to improve the anti-diabetic effect by using a fermentation technique applying excellent microorganisms using natural materials or natural materials known to be effective. As an example, red ginseng has an antidiabetic effect but its effect is weak. However, the present inventors have found a phenomenon that the antidiabetic effect is significantly increased when fermented using the microorganisms fermenting Cheonggukjang, and has obtained a patent on a method for manufacturing red ginseng Cheonggukjang with enhanced antidiabetic effect (Patent No. 10-0606241). The present invention is to improve the blood sugar lowering effect by improving the above patent.

In addition, the present inventors have observed that the blood lipids are significantly improved along with the decrease in blood glucose as a result of feeding powder of Ligustrum obtusifolium fruit to rats induced with diabetes with streptozotocin with high fat diet. . Privet fruit is also called Namjeongsil. 13 species are known in Ligustrum plants, and 2 species are known in Korea. Ligustrum obtusifolium , which has fallen leaves in winter, is called Privet , and Ligustrum japonicum does not have leaves in winter. ( Ligustrum japonicum Thunberg) is called the "wood" and is also called the journey room, the trekker, and the town hall. The shape of the fruit is similar, but if you look closely, the size and shape are significantly different.

The present inventors prepared the powder and ethanol extracts from the wild leaves of Ligustrum lucidum Ait (made in China: Ligustrum lucidum Ait) and the ethanol extract, respectively, and compared the anti-diabetic effect of red ginseng chungkukjang In order to present the sex, the present invention has been achieved.

It is therefore an object of the present invention to provide an antidiabetic active pharmaceutical composition or functional food composition consisting of Ligustrum plant fruit powder or extract and red ginseng-containing Cheonggukjang powder,

An object of the present invention as described above is to produce a Ligustrum plant fruit powder or extract, and after preparing a composition mixed with these and red ginseng-containing Cheonggukjang powder, the Ligustrum plant fruit powder or extract alone or red ginseng A composition containing mixed Cheonggukjang powder was added to a normal diet and fed in diabetic-causing animals to identify hypoglycemic effects in diabetic animals fed a mixture of Ligustrum plant fruit powder or extract and red ginseng-containing Cheonggukjang powder. .

The present invention provides an anti-diabetic active composition consisting of Ligustrum plant fruit powder or extract and red ginseng-containing Cheonggukjang powder.

The Ligustrum plant fruit powder or extract according to the present invention is Ligustrum obtusifolium or Ligustrum japonicum Thunberg fruit powder or extract.

The Ligustrum obtusifolium fruit powder or extract according to the present invention is composed of a ratio of 1: 1 with red ginseng-containing Cheonggukjang powder.

Ligustrum japonicum Thunberg fruit powder or extract according to the present invention is composed of a powder of red ginseng-containing Cheonggukjang powder in a ratio of 0.5: 1.

The red ginseng-containing Chungkookjang powder according to the present invention is 40% red ginseng-containing Chungkookjang powder.

The present invention also provides a pharmaceutical composition for preventing or treating diabetes comprising Ligustrum plant fruit powder or extract and red ginseng-containing Cheonggukjang powder as an active ingredient.

The present invention also provides an anti-diabetic active food composition comprising Ligustrum plant fruit powder or extract and red ginseng-containing Chungkookjang powder as an active ingredient.

In the present invention, the term "active ingredient" refers to a substance or a group of substances (a pharmacologically active ingredient or the like, which is expected to express the efficacy or effect of the drug directly or indirectly by intrinsic pharmacological action). It means containing a main component).

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Pharmaceutical dosage forms of the pharmaceutical compositions of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection.

As defined herein, "health functional food" means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to Act No. 6767 of the Health Functional Food Act, and "functional" means It means ingestion for the purpose of obtaining useful effects on health use such as nutrient control or physiological action on structure and function.

In addition, it is possible to manufacture and process as a dietary supplement in the form of tablets, capsules, powders, granules, liquids, pills and the like for the purpose of antidiabetic activity of the present invention.

Anti-diabetic active composition consisting of Ligustrum plant fruit powder or extract and red ginseng-containing Cheonggukjang powder according to the present invention can significantly lower blood sugar in diabetic-induced animals, which is very effective in preventing and treating diabetes. .

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the following examples. However, the present invention is not limited to these examples.

Example

Ligustrum obtusifolium and Ligustrum japonicum Thunberg fruits were collected from Jinyang-myeon, Gyeongsangbuk-do.

The experimental diet was prepared based on the Rodent AIN-76 diet (Teklad, USA). The experimental group is shown in Table 1.

TABLE 1

¹⁾ Experimental group for comparison with Patent No. 10-0606241.

²⁾ Additive ratio of pellets and mineral fruits, ethanol extract and red ginseng chungkukjang (%).

The experimental animals were adapted for 1 week with Sprague-Dawly SPF / VAF outbred rats (Orient Ltd., Sungnamsi, Korea) with an average weight of 140 ± 10 g at 5 weeks of age. After normal group (NC), diabetic control group (DM), 20% of red ginseng after diabetic induction, Cheonggukjang diet group (RC), after the induction of diabetic fruit powder diet group (JP), after the induction of diabetic fruit ethanol extract diet group (JE), after the induction of diabetes, rod fruit powder: 40% of red ginseng Cheonggukjang 1: 1 mixed diet (JPR), after the induction of diabetic fruit ethanol extract and 40% of red ginseng Cheonggukjang 0.5: 1 mixed diet group (JER) , After the induction of diabetic fruit powder diet group (KP), after the induction of diabetic fruit ethanol extract diet group (KE), after the induction of diabetic fruit powder and red ginseng containing 40% Cheonggukjang 1: 1 mixed diet group (KPR), diabetes induction 11 kinds of dietary group (KER) mixed with ethanol extract and 40% of red ginseng As it was divided.

The amount of powdered material, ethanol extract and red ginseng cheongukjang containing them was added in the range of 0.5-1% (w / w) to the basic diet. The number of experimental animals was kept for 6 weeks with 7 animals per experimental group. The aviary used steel cages, the temperature and humidity were adjusted to 23 ± 2 ° C and 60 ± 5%, the contrast period was set at 12 hour intervals, and water and feed intake were freely consumed.

Example 1 Preparation of Experimental Diet

Example 1-1 Diet of Privet Fruit and Mineral Tree Powder

Prison and mineral fruits were washed with running tap water, steamed in a cooker for 10 minutes, and dried for 24 hours using a blow dryer at 40 ° C. Next, using a blender, grind to 50-60 mesh particle size, and mix with Rodent's AIN-76 Basic Diet (Teklad, USA) to 1% (w / w), respectively. And mineral fruit powder (KP) pellet feed was prepared.

Example 1-2: Ethanol Extract Diet

Prison and mineral fruits were washed with running tap water, steamed in a cooker for 10 minutes, and dried for 24 hours using a blow dryer at 40 ° C. Next, the mixture was pulverized to a 50-60 mesh particle size using a blender, and 10% of 70% ethanol solution was added thereto, and the mixture was boiled for 3 hours using an extraction tank equipped with a cooler and filtered. The operation of extracting by adding 5 times the amount of 70% ethanol to the remaining residue was repeated twice. All extracts were combined and dried under reduced pressure at 40 ° C. The yield of the reduced pressure dried product was 50.02% of the raw material powder. Ethanol extract diet was prepared by mixing 0.5% (w / w) in the Rodent AIN-76 basic diet, respectively, to prepare pellets of pellets (JE) and berry powder (KE).

Example 1-3 Diet of Red Ginseng Cheonggukjang Mixed with Primrose Fruit Powder and Mineral Tree Powder

Root and mineral fruit powders to be mixed with red ginseng cheonggukjang are washed in the same way as in the past with pristine and mineral fruit under running tap water, steamed in a cooker for 10 minutes, and then blown with a blow dryer at 40 ℃ for 24 hours. After drying, the mixture was ground to a 50-60 mesh particle size using a blender.

The red ginseng chungkukjang soaks and increases the soybeans and mixes the red bean and 6-year-old red ginseng at a ratio of 60:40 (w / w), fermented at 40 ° C for 70 hours, and then dried sufficiently at 40 ° C and pulverized it. Was prepared.

Next, the ground pellets powder and red ginseng Cheongguk powder were mixed 1: 1, and the light wood fruit powder was also mixed with red ginseng Cheongguk powder 1: 1. This mixture was mixed with Rodent's AIN-76 Basic Diet 1% (w / w), respectively, and added Red Ginseng Berry Powder (JPR) and Red Ginseng Soybean Paste (KPR) Pellet feed was prepared.

Example 1-4: Red Ginseng Cheonggukjang Diet Mixed with Primrose Fruit Ethanol Extract and Mine Fruit Berry Ethanol Extract

Pritrose fruits and mineral fruits were washed with running tap water, steamed in a cooker for 10 minutes, and dried for 24 hours using a blow dryer at 40 ° C. Next, the mixture was pulverized to a 50-60 mesh particle size using a blender, and 10% of 70% ethanol solution was added thereto, and the mixture was boiled for 3 hours using an extraction tank equipped with a cooler and filtered. The operation of extracting by adding 5 times the amount of 70% ethanol to the remaining residue was repeated twice. All the extracts were combined and dried under reduced pressure at 40 ° C.

The red ginseng chungkukjang soaks and increases the soybeans and mixes the red bean and 6-year-old red ginseng at a ratio of 60:40 (w / w), fermented at 40 ° C for 70 hours, and then dried sufficiently at 40 ° C and pulverized it. Was prepared.

Next, the extract concentrated concentrated ethanol extract ethanol extract and red ginseng chungkukjang powder were mixed at 0.5: 1, and the bark berry ethanol extract and red ginseng chungkukjang powder were mixed at 0.5: 1. The red ginseng Cheongguk diet (KER), which is a mixture of Rodent AIN-76 (1% (w / w)) and the wild berry extract ethanol extract (JER) and the red barberry ethanol extract, was mixed. ) Pellet feed was prepared.

Example 2 Diabetes Induction

Streptozotocin (STZ, Sigma Chem. Co. Mo, USA) was dissolved in 0.1 M citrate buffer (pH 4.3) and injected into the femoral muscle at 55 mg / kg concentration in experimental animals weighing 250 g or more. Animals with a blood glucose level of 300 mg / dL or more taken from the tail vein on an empty stomach 48 hours after administration are used for the experiment.

Example 3 Blood Sugar Analysis

In order to investigate the hypoglycemic effect of the pellets and the fruits of the rat tree in diabetes, the blood glucose content was compared after dividing into 11 groups as shown in Table 1 and breeding for 6 weeks using 7 rats per group (Table 2). Blood glucose of the experimental animals was measured by using glucotester (Gluco-Tester: Life Scan lnc., USA) at 10-12 am daily with blood from the blood vessels of the tail.

The normal group (NC), which did not induce diabetes, received only the basic diet without adding the experimental diet, and showed 102-104 mg / dL without any significant difference between the blood sugar at the 0th week and the 6th week. The diabetic control group (DM) was fed only the basic diet for 6 weeks after diabetes induction. At 0 weeks, blood sugar was 484 mg / dL and at 6 weeks, 427 mg / dL decreased 11.78%.

In case of feeding rattle fruit powder to 1% of basic diet and feeding diabetic rats for 6 weeks (JP), blood sugar at week 0 was 495 mg / dL, but at week 6, 245 mg / dL was 50.51% compared to week 0. Decreased. These results indicate that the blood sugar reduction rate is lower than 53.52% when the red ginseng cheongukjang (Patent No. 10-0606241), which is a conventional experimental result of the present invention, is higher than the blood sugar reduction rate of 53.52% for 6 weeks. When the ethanol extract extracted from the rodent fruit was the same as the amount added to the basic diet (0.5%, w / w), the blood sugar of the 6th week was 250 mg / dL, which was 47.59% lower than the 0th week. The reduction rate was slightly lower than that.

JPR and JER mixed with Primrose Fruit Powder or its extract and Cheonggukjang Powder containing 40% of red ginseng in 1% of the basic diet were reduced by 78.79% and 72.75%, respectively, when 6 weeks were fed. In particular, JPR was restored to normal level. This result is more effective than the red ginseng Cheonggukjang (Patent No. 10-0606241) containing 20% of red ginseng, which is considered to be a significant contributor to the treatment of diabetes.

The mineral fruit powder, its ethanol extract and the red ginseng cheonggukjang added with these were slightly less than the effects of the pome fruit but showed almost the same effect, especially when added to the cheonggukjang containing 40% of red ginseng. Indicated. The exact hypoglycemic mechanisms of diabetes mellitus and mineral fruit in the diabetic tree are not known, but preliminary results suggest that high antioxidant effects and effects of functional ingredients such as ulsoric acid are needed.

TABLE 2

Effect of Diets of Primrose Fruit, Mineral Fruit and Red Ginseng Cheonggukjang Containing It on Blood Glucose Content (mg / dL)

¹⁾ The abbreviations of the experimental groups are shown in Table 1.

²⁾ RC is red ginseng Cheongguk fermented by adding 20% red ginseng: Experimental group to compare with patent No. 10-0606241.

³⁾ Data are mean ± standard deviation of 7 experimental animals.

Claims (7)

Anti-diabetic active functional food composition, characterized in that the composition of the fermented powder of the extract of Ligustrum obtusifolium or Ligustrum japonicum Thunberg plant fruit powder and 40% of red ginseng in 0.5 ~ 1: 1. delete delete delete delete delete delete