KR101559130B1 - Composition comprising herbal extract for preventing or treating obesity - Google Patents

Abstract

The present invention relates to a composition for preventing or treating obesity comprising an extract of a medicinal herb as an active ingredient. (A) a mixed extract of mahwang, rhubarb and fennel according to the present invention; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, Has been shown to be effective in lowering the body weight and fat tissue weight, improving dietary efficiency, and reducing total cholesterol, LDL-cholesterol and triglyceride levels in the blood, without causing liver toxicity in high fat diet-induced obese animal models And may be useful for prevention or treatment of obesity.

Description

[0001] The present invention relates to a composition for preventing or treating obesity, which comprises an extract of a medicinal herb as an active ingredient,

The present invention relates to a composition for preventing or treating obesity comprising an extract of a medicinal herb as an active ingredient.

Recent changes in diet and eating habits have been accompanied by changes in economic growth and lifestyle. Especially, busy modern people are overweight and obese because of high calorie diet such as fast food and low exercise amount. Weight overweight means that the body mass index (BMI, obesity index divided by the square of body weight) is 25 or more and less than 30, and obesity means the body mass index is 30 or more.

Two-thirds of US adults are overweight or obese, and overweight increases blood pressure and cholesterol levels, increasing the incidence of various adult diseases, including heart disease. The effects of obesity on various diseases are so severe that it is known that 80% of diabetics worldwide and 21% of heart disease are caused by obesity. In addition, various cancers such as uterine cancer, kidney cancer, and breast cancer are directly or indirectly related to obesity. Obesity and overweight also have higher rates of sleep apnea, osteoarthritis, and cholelithiasis (Stein CJ, Colditz GA. The epidemic J Clin Endocrinol Metab 2004; 89: 2522-2525; Kopelman PG, Obesity as a medical problem, Nature 2000 Apr 6; 404 (6778): 635-43.).

Obesity is not caused by a single cause, but by a combination of genetic, environmental, social, and mental factors. Current methods of treating obesity include diet, exercise, and behavioral therapy, as well as methods of correcting lifestyle, drug therapy, and surgical treatment. Although active efforts should be made to correct lifestyle prior to medication or surgical treatment, it is not easy to correct lifestyle habits, and there is a limit to the weight that can be reduced by lifestyle correction alone. Therefore, in many cases, lifestyle correction and medication are necessary.

Although many anti-obesity agents are being developed every year for the treatment of obesity, there are not many obesity agents available at present, and most of them are limited to digestive or appetite-suppressing agents. In the case of digestive or appetite control agents, they are classified as psychotropic drugs because of habit, and digestion inhibitors show side effects such as diarrhea and constipation. Typical obesity treatment drugs approved for long-term use by the US FDA until 2010 include norepinephrine and sibutramine (Reductil), a substance that is secreted by the pancreas and digestive system, which has the function of suppressing reabsorption of serotonin There was orlistat (Xenical), which has the effect of inhibiting lipase (Yanovski SZ, Yanovski JA, Obesity, N Engl J Med 2002; 346: 591-602).

However, sibutramine has been withdrawn in the US and Korea in October 2010 due to the risk of cardiovascular events such as myocardial infarction and stroke, which are common with side effects such as elevated blood pressure, insomnia, dry mouth and dizziness. In addition, Orristat has been reported to have side effects such as diarrhea, fatty liver, loss of gold, etc. When the fat intake is lower than that of westerners like Koreans, the effect of the drug is not clear and its use is limited (Kim, Sangman. 1998; 7 (4): 287-92.).

Therefore, the inventors of the present invention have conducted research to overcome limitations of conventional drugs for obesity treatment and to develop a new drug having a high effect of inhibiting obesity and having few side effects. As a result, it was found that (a) a mixture of mahwang, extract; And (b) a mixture of licorice, rosemary, acid-fast food, health, ginseng, broiler, kojanggang, The present inventors completed the present invention by confirming that the mixed extract has an excellent anti-obesity effect.

An object of the present invention is to provide a pharmaceutical composition for preventing or treating obesity comprising an extract of a medicinal herb as an active ingredient.

Yet another object of the present invention is to provide a food composition for preventing or ameliorating obesity containing an extract of a medicinal herb as an active ingredient.

In order to accomplish the above object, the present invention provides a pharmaceutical composition comprising (a) a mixed extract of mahwang, rhubarb and fennel; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, As an active ingredient, a pharmaceutical composition for preventing or treating obesity.

The present invention also relates to a pharmaceutical composition comprising (a) a mixed extract of mahwang, rhubarb and fennel; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, As an active ingredient, a food composition for preventing or ameliorating obesity.

(A) a mixed extract of mahwang, rhubarb and fennel according to the present invention; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, Has been shown to be effective in lowering the body weight and fat tissue weight, improving dietary efficiency, and reducing total cholesterol, LDL-cholesterol and triglyceride levels in the blood, without causing liver toxicity in high fat diet-induced obese animal models And may be useful for prevention or treatment of obesity.

FIG. 1 shows the effect of the mixed extract of the present invention (DF-GSH) on body weight gain in an obesity animal model.
FIG. 2 is a graph showing the effect of the mixed extract of the present invention (DF-GSH) on the weight of the mesenteric adipose tissue (MAT) in the obese animal model.
FIG. 3 is a graph showing the effect of the mixed extract (DF-GSH) of the present invention on the weight of brown adipose tissue (BAT) in an obesity animal model.
FIG. 4 shows the effect of the mixed extract (DF-GSH) of the present invention on the dietary efficiency in an obesity animal model.
FIG. 5 shows the effect of the mixed extract of the present invention (DF-GSH) on blood AST levels in an obesity animal model.
FIG. 6 shows the effect of the mixed extract of the present invention (DF-GSH) on blood ALT levels in an obesity animal model.
FIG. 7 is a graph showing the effect of the mixed extract (DF-GSH) of the present invention on blood total cholesterol concentration in an obesity animal model.
8 is a graph showing the effect of the mixed extract (DF-GSH) of the present invention on blood LDL-cholesterol concentration in an obesity animal model.
FIG. 9 is a graph showing the effect of the mixed extract (DF-GSH) of the present invention on blood triglyceride concentration in an obesity animal model.

(A) a mixed extract of mahwang, rhubarb and fennel; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, As an active ingredient, a composition for preventing or treating obesity.

The composition comprises a pharmaceutical composition or a food composition.

Hereinafter, the present invention will be described in more detail.

In the present invention, the 'extract' is extracted from each herbal medicine using an appropriate solvent and includes, for example, a crude extract, a polar solvent-soluble extract or a non-polar solvent-soluble extract. In addition, the above-mentioned extract is a concept that includes all extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

(A) a mixed extract of mahwang, rhubarb, and fennel, which is an active ingredient in the composition of the present invention; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, Can be obtained as follows.

First, the Chinese cabbage, rhubarb, and shoots are washed with water to remove impurities, followed by drying and crushing in the shade. Maquiladense, rhubarb and grasses may be cultivated or marketed without restriction. After mixing the ground parchment, rhubarb and rhizome, an appropriate amount of solvent is added to allow complete immersion. As the extraction solvent, any inorganic or organic solvent generally used in the art can be used without limitation, and a solvent selected from water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof is preferable. Next, the mixed extracts of the above-mentioned saccharin, rhubarb and grass extracts are filtered and concentrated under reduced pressure to obtain a mixed extract of Daphnia magna, Rhodiola and Rhizoma (DF). In one embodiment of the present invention, a mixed powder of mahwang, rhubarb and grass powder was dissolved in water and used. The above-mentioned mixed extract (DF) can be obtained by mixing and mixing preferably a mixture of 30% to 50%: 10% to 30%: 30% to 50% of mahwang, rhubarb and grass, Squalene, rhubarb, and seedlings are mixed at a weight ratio of 40%: 20%: 40% and extracted. When the mixing ratio is out of the above range, there is no significant difference in the effect of treating obesity as compared with the case of using alone or in combination with magpie, rhubarb, or shoot, and there is a fear of side effects. Is the most excellent.

In addition, the foreign substances were removed by washing with water, respectively, and then dried in the shade, and then dried in a shade And dried. There are no restrictions on cultivated or marketed products such as licorice, herbivores, farm animals, health, bokyeong, broiler, kyangyang, corporation, gyeraceae, bacon, Can be used. After mixing the pulverized licorice, the herbalist, the poultry, the health, the spirit, the broiler, the goat, the construction worker, the persimmon, the woodchuck, Baekdugu, To allow complete immersion. As the extraction solvent, any inorganic or organic solvent generally used in the art can be used without limitation, and a solvent selected from water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof is preferable. After filtering the mixed extracts of licorice, herbivorous, acid-fast food, health, ginseng, broiler, coriander, persimmon, persimmon, kaisan, Baekdugu, Extract of gamitosechelen (GSH), a mixed extract of licorice, herbivorous, wild ginseng, health, bokyeong, broiler, kyangyang, ). In one embodiment of the present invention, a mixed powder of licorice, herbivorous, acid-fast food, health, ginseng, broiler, coriander, corn, persimmon, . The extract of the above-mentioned chamomile body (GSH) is preferably selected from the group consisting of licorice, herbivorous herbaceous shrubs, health, bamboo shoots, broiler chestnuts, : 16% to 21%: 12% to 18%: 6% to 11%: 4% to 8%: 4% to 8%: 4% to 8%: 2% to 5%: 2% to 5% 2% to 5%: 2% to 5%: 2% to 5%: 2% to 5%: 2% to 5% %: 0.5 to 1.5% by weight, and then extracting the mixture. More preferably, they are mixed at a weight ratio shown in the following Table 1 and extracted. When the mixing ratio is out of the above range, there is no significant difference in the effect of treating obesity as compared with the case where each herbal medicine is used singly, and there is a fear of side effects. Do.

Then, DF and GSH obtained through the above process are mixed preferably in a weight ratio of 1: 0.5 to 1.5, more preferably in a weight ratio of 1: 1, and finally, the active ingredient of the present invention, DF- GSH.

(A) a mixed extract of mahwang, rhubarb and fennel according to the present invention; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, Has been shown to be effective in lowering the body weight and fat tissue weight, improving dietary efficiency, and reducing total cholesterol, LDL-cholesterol and triglyceride levels in the blood, without causing liver toxicity in high fat diet-induced obese animal models Have.

As described above, the mixed extract according to the present invention is excellent in anti-obesity effect and thus can be used as a medicament useful for prevention or treatment of obesity or as a health functional food.

The composition of the present invention may further comprise at least one known active ingredient having an anti-obesity effect together with DF-GSH.

The compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions. In addition, it can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, oral preparations such as syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to a conventional method. Suitable formulations known in the art are preferably those as disclosed in Remington ' s Pharmaceutical Science, recently, Mack Publishing Company, Easton PA.

Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When the composition is formulated, it is prepared using a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, or an excipient usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which are prepared by mixing at least one excipient such as starch, calcium carbonate, sucrose, lactose, It is prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of suppository bases include withexol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The term "administering" as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the individual, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. For example, for a desired effect, the DF-GSH of the present invention can be administered in an amount of 0.1 to 10000 mg / kg (body weight) per day. The composition may be administered once a day, or divided into several doses.

The compositions of the present invention may be administered to a subject in a variety of routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection.

The composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers for the prevention and treatment of obesity.

In the present invention, the term 'health functional food' refers to a food having a biological control function such as prevention and treatment of disease, bio-defense, immunity, recovery of post-mortem and aging inhibition.

The mixed extract of the present invention can be added to a health functional food for the purpose of preventing or improving obesity. When the mixed extract of the present invention is used as a food additive, the mixed extract can be directly added or used together with other food or food ingredients, and can be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, the mixed extract of the present invention is added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material, in the production of food or beverage. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the type of food that the composition of the present invention can contain. Examples of the food to which the mixed extract of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, , A drink, an alcoholic beverage, and a vitamin complex, all of which include health foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharine and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may comprise flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments and experimental examples are provided to facilitate understanding of the present invention. However, the following examples and experimental examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples and experimental examples.

Example 1. Preparation of mixed extract of herbal medicines

1-1. Manufacture of mixed extracts of mahwang, rhubarb and rhizome

Maehwang, rhubarb and grass were purchased from Hwangrim Pharmaceutical (Busan, South Korea), and each was cleaned with water to remove impurities, followed by drying and pulverizing at room temperature. The ground squash, rhubarb and grass powder were mixed at a weight ratio of 40:20:40, and then dissolved in water to obtain a mixed extract of magpie, rhubarb and shoot. The mixed extract of mahwang, rhubarb and rhizome was named DF.

1-2. Preparation of the Gamisorcene ring

Licorice, herbivorous, wild ginseng, health, bokyeong, broiler, kyangyang, corporation, gyeongsil, myeonbyeol, Baekdugu, , And each of them was cleaned with water to remove impurities, followed by drying and pulverizing at room temperature. The weight ratio of the licorice powder, the herbal supplement, the poultry meat, the health, the bamboo shoot, the broiler, the gyeongganggang, the construction worker, The mixture was then dissolved in water to obtain a mixed extract of licorice, herbal supplement, wild ginseng, health, bamboo shoot, broiler, coriander, coriander, persimmon, The mixture was designated as a gumic body ring (GSH).

Chinese medicine name Ingredient weight% licorice Glycyrrhizae Radix 18.4 Herbalist Cyperi Rhizoma 15.3 Mountain feeding Zingiberis Rhizoma 8.6 health Crataegi Fructus 6.0 Boryeong Poria 6.0 Broiler Cinnamomi Cortex 6.0 Kohyanggang Alpiniae officinarum Rhizoma 3.7 Construction person Amomi Fructus 3.7 House Aurantii Fructus immaturus 3.7 elecampane Aucklandiae Radix 3.7 Baekdugu Amomi Fructus rotundus 3.7 Sancho Zanthoxyli Pericarpium 3.7 Let's go Chebulae Fructus 3.7 Boiling Atractylodis macrocephalae Rhizoma 3.7 Petal Piperis longi Fructus 3.7 Wow Pogostemonis / Agastaches Herba 3.7 cloves Caryophylli Flos 1.8 Park Menthae haplocalycis Herba 0.9 Total amounts 100

1-3. Manufacture of mixed extracts of medicinal herbs

The mixed extracts obtained in Examples 1-1 and 1-2 were mixed at a weight ratio of 1: 1 to prepare a mixed extract of the herbal medicines. Hereinafter, (a) a mixed extract of mahwang, rhubarb and rhizome; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, Was designated as DF-GSH.

Experimental Example 1. Experimental Design for Verifying the Effect of Obesity Treatment in an Animal Model

1-1. Experimental animal

A 7-week-old C57BL / 6N male mouse, supplied by Sam Taco Co., Ltd. (Korea), was purchased and adapted to the laboratory environment for 1 week. During the experimental period, the temperature was 21 ± 2 ℃, the humidity was 55 ± 5%, the ventilation frequency was 15 ~ 17 times / hour, illumination was 150 ~ 300 lux, (Harlan, USA) and water were fed ad libitum in a laboratory environment with a cycle.

1-2. Experimental group and drug administration

Nine of each group were randomly divided into groups according to body weight. The control group and the experimental group (DF-GSH) fed a high-fat diet containing 45 Kcal% fat to provide obesity for 8 weeks, while the normal group was fed a general diet containing 10 Kcal% fat. Respectively. Then, water was orally administered to the control group and DF-GSH (DF (40 mg / kg) + GSH (40 mg / kg)) obtained in Example 1 at 80 mg / kg was orally administered for 8 weeks.

Experimental Example 2. Measurement of weight gain

To determine the effect of DF-GSH according to the present invention on body weight gain, body weight was measured twice weekly for 8 weeks during the administration of the drug. The results are shown in Fig.

As shown in FIG. 1, the weight gain was significantly increased in the control group compared to the normal group, but the DF-GSH administration group showed a statistically significant decrease in body weight gain compared to the control group.

Experimental Example 3. Measurement of fat tissue

After completion of the experiment, each mouse was autopsied to separate mesenteric adipose tissue (MAT) and brown adipose tissue (BAT) around the mesentery, and then weighed. The results are shown in FIG. 2 to FIG.

As shown in FIG. 2, the weight of the mesenteric adipose tissue (MAT) in the control group was significantly increased in the control group, but the weight of the MAT was statistically significantly decreased in the DF-GSH treated group compared to the control group Respectively.

As shown in FIG. 3, the weight of brown adipose tissue (BAT) was significantly increased in the control group compared to the normal group, but the weight of BAT was significantly decreased in DF-GSH treated group compared to the control group Respectively.

Experimental Example 4. Measurement of Dietary Efficacy

To investigate the effect of DF-GSH according to the present invention on the appetite, the body weight was measured twice a week, the feed intake was measured once a week, and the dietary efficiency was calculated based on this. Dietary efficiency was calculated by dividing the final weight gain by the total feed intake by substituting into the formula below. The results are shown in Fig.

* Feeding efficiency ratio (FER%) = weight gain (g) / feed intake (g) × 1009,10)

As shown in FIG. 4, the dietary efficiency of the control group was higher than that of the normal group, but the DF-GSH administration group showed a statistically significant decrease in the dietary efficiency as compared with the control group. The low diet efficiency means that the body weight gain is small despite the high feed intake. As a result, it was confirmed that the DF-GSH of the present invention has excellent obesity control effect.

Experimental Example 5. Blood Biochemical Analysis

After the end of the experiment, each mouse was fasted for 12 hours for blood sampling, anesthetized with diethyl ether, and then 1 ml of blood was collected from the heart. The blood was allowed to stand at room temperature for 30 minutes or more, and centrifuged at 3000 rpm for 10 minutes using a high-speed centrifuge (Micro12, Hanil, Korea) to separate plasma. Separated plasma was stored in a freezer (-20 ° C) and analyzed using AST (asparate aminotransferase), alanine aminotransferase (ALT), total cholesterol, LDL-cholesterol low density lipoprotein-cholesterol) and triglyceride were measured. The results are shown in Figs. 5 to 9. Fig.

As shown in FIGS. 5 to 9, in the DF-GSH treated group, plasma AST and ALT levels, which are indicators of liver damage, were not statistically different from the control group, but the concentrations of total cholesterol, LDL-cholesterol and triglyceride Compared with the control group.

Through the above experimental results, it was found that (a) the mixed extract of mahwah, rhubarb and fennel of the present invention; And (b) an extract of a mixture of gamiticomycetes, which is a mixture of licorice, herbivorous, wild ginseng, health, ginseng, broiler, coriander, cormorant, Is a safe substance without liver toxicity, but it reduces weight and fat tissue weight in obese animal models, improves dietary efficiency, reduces blood total cholesterol, LDL-cholesterol and triglyceride levels Lipid metabolism and improved anti - obesity effect.

Hereinafter, the pharmaceutical composition of the present invention and the pharmaceutical composition of the food composition will be described, but the present invention is not intended to be limited but is specifically described .

Formulation Example 1. Preparation of pharmaceutical preparations

1. Manufacturing of powder

DF-GSH 20 mg

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

2. Preparation of tablets

DF-GSH 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

3. Preparation of capsules

DF-GSH 10 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

4. Preparation of injections

DF-GSH 10 mg

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO ₄ 2H ₂ O 26 mg

(2 ml) per 1 ampoule in accordance with the usual injection preparation method.

5. Manufacture of liquids

DF-GSH 20 mg

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was added with purified water to adjust the total volume to 100 ml, And sterilized to prepare a liquid preparation.

Formulation Example 2. Preparation of food preparation

1. Manufacture of health food

DF-GSH 100 mg

Vitamin mixture quantity

70 g of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

0.2 g of vitamin B12

Vitamin C 10 mg

Biotin 10 g

Nicotinic acid amide 1.7 mg

Folate 50 g

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

2. Manufacture of health drinks

DF-GSH 100 mg

Vitamin C 15 g

Vitamin E (powder) 100 g

19.75 g of ferrous lactate

3.5 g of zinc oxide

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B1 0.25 g

Vitamin B2 0.3 g

Water quantification

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The resulting solution was filtered and sterilized in a sterilized 2 liter container, ≪ / RTI >

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (6)

(a) a mixed extract of mahwang, rhubarb and rhizome; And
(b) an extract of a mixture of licorice root, a mixture of licorice, herbivorous, wild-type, health, spirit, broiler, coriander, coriander, persimmon, A pharmaceutical composition for the prevention or treatment of obesity comprising as an active ingredient.
The pharmaceutical composition for preventing or treating obesity according to claim 1, wherein the extract is extracted with at least one solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof.
The preventive or therapeutic agent for obesity according to any one of claims 1 to 3, wherein the ratio (b) is 10 to 30%: 30 to 50% A pharmaceutical composition.
The method according to claim 1, wherein the amphibian body of (b) is selected from the group consisting of licorice, herbivorous, wild-caught, health, benthic, broiler, And 4% to 8%: 4% to 8%: 2% to 5%: 2% to 5%: 6% to 11% : 2% to 5%: 2% to 5%: 2% to 5%: 2% to 5%: 2% to 5% To 3.5%: 0.5 to 1.5% by weight, based on the total weight of the composition.
The composition for preventing or treating obesity according to claim 1, wherein the composition comprises, as an active ingredient, a mixture of the mixed extract of (a) and the extract of the chamomile body of (b) in a weight ratio of 1: 0.5 to 1.5. A pharmaceutical composition for therapeutic use.
(a) a mixed extract of mahwang, rhubarb and rhizome; And
(b) an extract of a mixture of licorice root, a mixture of licorice, herbivorous, wild-type, health, spirit, broiler, coriander, coriander, persimmon, A food composition for preventing or ameliorating obesity as an active ingredient.

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