CN101913941B - Antitranspirant foliar fertilizer and preparation method thereof - Google Patents
Antitranspirant foliar fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an antitranspirant foliar fertilizer and a preparation method thereof. The antitranspirant foliar fertilizer comprises the raw materials of copper sulfate, ethylene diamine tetraacetic acid, monopotassium phosphate, zinc sulfate, ferrous sulphate, manganese sulphate, ammonium molybdate, composite rare earth, boric acid, sodium dodecyl sulphonate, urea, fulvic acid, hexanoic acid 2-(diethylamino) ethyl ester, compound amino acid, magnesium sulfate, calcium nitrate, ethylene diamine tetraacetic acid, microbial agent, azone and ammonia water. The antitranspirant foliar fertilizer provides nutrient elements and microbial agents which are required by the growth of plants, the rare earth and amino acid and other beneficial elements, can promote the growth of the plants, improve the yield, the quality, the stress resistance of the plants and the absorbing and operating capabilities on trace elements, simultaneously can effectively reduce the transpiration of the plants and is applied to afforesting in arid regions.
Description
Technical field
The invention belongs to ecology (agroforestry) technical field, relate to a kind of anti-rising foliage fertilizer and preparation method thereof.
Background technology
China's semiarid zone average annual precipitation is 250-550mm, regional extent is wide, roughly from the Tongliao in northeast, the Zhangbei County through Hebei, the wild goose north in Shanxi, the north in Shaanxi, the west of Southern Ningxia, sea, solid, the Dingxi in Gansu, the cajaput in Qinghai, Golog, until the Lhasa in Tibet accounts for 20% of area.Particularly in the Semi-arid Loess Plateau area, zonal vegetation degree to the utmost is degenerated, the soil quality severe exacerbation, and soil erosion is very serious.
Conceding the land to forestry is a strategic measure in soil conservation, the improvement of the ecological environment.But because this area exists precipitation relative deficiency, special climate condition that potential evapotranspiration power is large, cause the surrival rate of afforestation low, preserve rate variance, the increase in standing timber is low, it is very unstable to grow, even causes local soil mummification problem, makes artificial afforestration run into great difficulty.Therefore, the loess hill area in the water resources serious deficiency takes full advantage of limited Water Resources, improves the surrival rate of afforestation, storage rate, promotes tree growth, recovers forest vegetation, improves the ecological environment, and has become the major issue that needs to be resolved hurrily.
The moisture that root system of plant absorbs, only have 1% to be used for metabolism and to grow, and 99% moisture loses through transpiration, yet plant transpiration effect loss moisture and not exclusively be that to keep its life necessary, therefore, applied chemistry medicament control plant physiology process can significantly reduce Transpiration Intensity.Reduce transpiration rate, can improve the water use efficiency of forest, strengthen the ability that it resists arid, keep water balance in the nursery stock body, improve the surviving rate of afforestation, promote growing of plant.
Plant, also needs from extraneous absorbed energy and material except needs moisture for keeping normal physiological metabolism activity, and the demand that in time satisfies Plant To Nutrient is the basic substance that guarantees the plant health growth.Nutritive element in the soil consists of the primary condition of growth and development of plants, and most of nutritive element is transformed by soil fixing, is difficult to absorb, and only has a small amount of ionic state nutritive element can be by the plant absorbing trans-utilization.Studies show that nutritive element has two kinds of approach to be utilized by plant absorbing, the first is by Root Absorption nutrition, and it two is cauline leaf Absorbing Nutrients by plant.
Foliage fertilizer is that nutritive element is applied to the farm crop blade surface, brings into play the fertilizer of its function by the absorption of blade, and it originates from France the earliest, and the application and research in agriculture production has a long history.Because arid, root system of plant is obstructed to nutrient uptake, suitably takes foliage-spray, can supplement the nutrients rapidly, satisfies the needs of growth and development of plants, particularly in the impaired situation of the seedling-slowing stage fine-root of afforestation nursery stock.Foliage fertilizer absorbs soon, effect is strong, low-consuming, efficient is high, can also avoid being had economic worth and social benefit by soil fixing, pollution abatement, raising fertilizer efficiency.
Foliage fertilizer is of a great variety at present, and be divided into Four types: 1) hormone-type, main component are plant-growth regulator; 2) nutrient type, main component are macroelement, micronutrient element, and some organic acid substances; 3) biotype contains vinelandii, and yeast divides the bacterial classification of phosphorus decomposing, potassium etc. and some are to soil improvement and the favourable flora of plant growth; 4) comprehensive, when containing nutrient and plant modifying agent, also be added with agricultural chemicals, microbial preparation etc.
Along with going deep into of foliage fertilizer applied research, foliage fertilizer develops into present multiclass composite foliage fertilizer from initial single type foliage fertilizer.But the development for the foliage fertilizer of special geological surrounding and special purpose is also fewer, the antitranspirant that namely uses now only is by the impact of blade face pore being reduced the transpiration of plant, and composite foliage fertilizer is the main direction of novel anti-rising foliage fertilizer performance application potential aspect improving forest resistance, promoting the recovery of forest vitality.But, the research that also antitranspirant is not combined with foliage fertilizer at present, constantly perfect along with the continuous expansion of the range of application of antitranspirant and foliage fertilizer formulation, develop the novel anti-rising foliage fertilizer that is more suitable for the forest characteristics, become one of urgent Task of the composite foliage fertilizer that is applied to forest.
In addition, the foliage fertilizer that uses in the market mainly exists low, the middle amount of macroelement content and micronutrient levels is incomplete, lack biological organic substance, growth regulator mostly is the shortcomings such as chemical reagent, unreasonable, the easy generation precipitation of formulation or demixing phenomenon.
For example: publication number be CN 1631850A Patent Application Publication a kind of biological foliage fertilizer and preparation method thereof, this foliage fertilizer contains chemical reagent, biological organic matter, sequestrant, permeate agent and microbial fermentation solution, its solid material and weight proportion are: urea 50～55%, potassium primary phosphate 20～25%, Repone K 7～8%, boric acid 0.3～0.5%, ferrous sulfate 0.2～0.4%, copper sulfate 0.1～0.2%, nitrocalcite 0.5～1.5%, sal epsom 0.2～0.4%, zinc sulfate 0.2～0.4%, manganous sulfate 0.2～0.3%, ammonium molybdate 0.05～0.10%, xanthohumic acid 14～15%, EDTA sequestrant 1～2%, AEO-20 permeate agent 0.05～0.10%; The above-mentioned raw materials total amount with after 60～70 ℃ water mixes in reactor with 2: 3 ratio, is added microbial fermentation solution with filtering the ratio of gained liquid with per kilogram 0.5～1ml, adjust again pH value to 5.0～6.0, and get final product.Although this foliage fertilizer macroelement content is high, and contain the meta-bolites that microbial fermentation produces, but owing to the nutritive element that growth and development of plants in this foliage fertilizer is required is not comprehensive, the foliage fertilizer of preparation is unstable, produces easily precipitation, affects the service efficiency of foliage fertilizer, use a little less than the substance metabolism enhancing ability of plant behind this foliage fertilizer, a little less than the absorption and running ability of plant to nitrogen, phosphorus, potassium and trace element, the resistance of plant is poor, does not have the effect of anti-transpiration.
Summary of the invention
Primary and foremost purpose of the present invention is to provide a kind of anti-rising foliage fertilizer and preparation method thereof for the problem that above-mentioned prior art exists, anti-rising foliage fertilizer of the present invention provides the required nutritive element of plant strain growth and microbiobacterial agent, the beneficial element such as rare earth, amino acid, can promote plant growth, improve output, improve quality, improve the resistance of plant and plant to absorption and the running ability of trace element, can effectively reduce the transpiration of plant again, be applicable to arid biogeographic zone afforestation.
For achieving the above object, one aspect of the present invention provides a kind of anti-rising foliage fertilizer to comprise following raw material: copper sulfate, ethylenediamine tetraacetic acid (EDTA), potassium primary phosphate, zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, sodium laurylsulfonate, urea, xanthohumic acid, diethylamino ethanol caproate, aminoacids complex, microbiobacterial agent, ammoniacal liquor.
Wherein, every 100ml foliage fertilizer contains the component of following proportioning:
Copper sulfate 1-5g
Ethylenediamine tetraacetic acid (EDTA) 20-40g
Zinc sulfate 4-10g
Ferrous sulfate 1-3g
Manganous sulfate 1-3g
Ammonium molybdate 0.5-1.5g
Compound rare-earth 1-10g
Boric acid 2-10g
Sodium laurylsulfonate 1-5g
Urea 20-60g
Xanthohumic acid 1-7g
Diethylamino ethanol caproate 0.5-2.5g
Aminoacids complex 5-20g
Potassium dihydrogen phosphate 15-25ml
Microbiobacterial agent 2-20ml
Surplus is ammonia soln.
Particularly, the sal epsom 1-3g that also contains following proportioning; Nitrocalcite 1-2g; Disodium ethylene diamine tetraacetate 2-7g; Azone 1-5ml.
Particularly, the proportion optimization of the component that contains of every 100ml foliage fertilizer is:
Copper sulfate 1-3g
Ethylenediamine tetraacetic acid (EDTA) 20-30g
Zinc sulfate 6-8g
Ferrous sulfate 2-3g
Manganous sulfate 2-3g
Ammonium molybdate 0.5-1g
Compound rare-earth 4-10g
Boric acid 7-10g
Sodium laurylsulfonate 2-3g
Urea 30-40g
Xanthohumic acid 3.5-4g
Diethylamino ethanol caproate 1-1.5g
Aminoacids complex 10-15g
Sal epsom 1.5-2g
Nitrocalcite 1-1.5g
Disodium ethylene diamine tetraacetate 4-5g
Potassium dihydrogen phosphate 20-25ml
Microbiobacterial agent 2-10ml
Azone 2-3ml
Surplus is ammonia soln.
Wherein, the total content of lanthanum trioxide, cerium oxide 〉=30% in the compound rare-earth; Aminoacids complex is by aspartic acid, Isoleucine, and Threonine, leucine, Serine, tyrosine, 4-L-glutamic acid, phenylalanine, glycine, Methionin, L-Ala, Histidine, Gelucystine, arginine, α-amino-isovaleric acid, proline(Pro), methionine(Met) forms; The mass percent concentration of potassium dihydrogen phosphate is 20-22.6%; Viable count in the microbiobacterial agent 〉=400,000,000/ml, organic mass percent concentration 〉=40%, the mass percent concentration of humic acids 〉=10%; The mass percent concentration of ammonia soln is 25-28%.
Particularly, described aminoacids complex Mid-Heaven Gate winter propylhomoserin, Isoleucine, Threonine, leucine, Serine, tyrosine, 4-L-glutamic acid, phenylalanine, glycine, Methionin, L-Ala, Histidine, Gelucystine, arginine, α-amino-isovaleric acid, proline(Pro), methionine(Met) weight part proportioning is 3.9-7.6: 0.5-4.2: 0.6-4.3: 2.2-5.9: 0.7-4.4: 0.6-3.0: 0.5-2.9: 1.8-5.5: 1.2-4.9: 1.6-5.3: 1.2-4.9: 0.6-4.3: 0.8-3.2: 3.8-7.5: 0.6-3.0: 0.9-4.6: 1.6-5.3.
Particularly, the agricultural rare-earth of the total content of described compound rare-earth selective oxidation lanthanum, cerium oxide 〉=30%; Viable bacteria is mainly vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria, endophytic Bacillus, subtilis, Jingyang streptomycete and lactobacillus bulgaricus in the described microbiobacterial agent; Organic mass percent concentration is preferably 40-60%, and the mass percent concentration of humic acids is preferably 10-40%.
The present invention provides a kind of preparation method of anti-rising foliage fertilizer on the other hand, comprises the step of carrying out in the following order:
1) with whole copper sulfate, ethylenediamine tetraacetic acid (EDTA), zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, xanthohumic acid, sodium laurylsulfonate, urea, diethylamino ethanol caproate, aminoacids complex, potassium dihydrogen phosphate joins in first ammonia soln successively, be heated to 45-60 ℃, be to mix under 45-60 ℃ in temperature, make solution A, wherein the consumption of first ammonia soln be the foliage fertilizer cumulative volume 1/5-3/5 (for example: prepare raw material according to proportioning as claimed in claim 2, during the anti-rising foliage fertilizer of preparation 1000ml, the consumption of first ammonia soln is 200-600ml; When preparing raw material according to proportioning as claimed in claim 2, during the anti-rising foliage fertilizer of preparation 100ml, the consumption of first ammonia soln is 20-60ml);
2) add successively the ammonia soln of whole azones and microbiobacterial agent and surplus in the solution A that makes, stir and get final product.
Wherein, temperature step 1) is preferably 45-50 ℃.
Another aspect of the invention provides a kind of preparation method of above-mentioned anti-rising foliage fertilizer, comprises the step of carrying out in the following order:
1) with whole copper sulfate, ethylenediamine tetraacetic acid (EDTA), zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, xanthohumic acid, sodium laurylsulfonate, urea, diethylamino ethanol caproate, aminoacids complex, potassium dihydrogen phosphate joins in first ammonia soln successively, airtight, be heated to 45-60 ℃, be to mix under 45-60 ℃ in temperature, make solution A, wherein the consumption of first ammonia soln be the foliage fertilizer overall solution volume 1/5-3/5 (for example: prepare raw material according to proportioning as claimed in claim 3, during the anti-rising foliage fertilizer of preparation 1000ml, the consumption of first ammonia soln is 200-600ml; When preparing raw material according to proportioning as claimed in claim 3, during the anti-rising foliage fertilizer of preparation 100ml, the consumption of first ammonia soln is 20-60ml);
2) whole sal epsom, disodium ethylene diamine tetraacetate, nitrocalcite are joined in the second batch ammonia soln successively, the temperature of heating and maintenance mixing solutions is 45-60 ℃, stir, make solution B, wherein the consumption of second batch ammonia soln be the foliage fertilizer overall solution volume 1/6-1/4 (for example: prepare raw material according to proportioning as claimed in claim 3, during the anti-rising foliage fertilizer of preparation 1000ml, the consumption of second batch ammonia soln is 166.7-250ml; When preparing raw material according to proportioning as claimed in claim 3, during the anti-rising foliage fertilizer of preparation 100ml, the consumption of first ammonia soln is 16.7-25ml);
3) solution A for preparing is joined solution B, mix, make solution C;
4) in the solution C that makes, add successively whole azones, microbiobacterial agent and surplus ammonia soln, stir and get final product.
Wherein, temperature is preferably 45-50 ℃ step 1), step 2); Step 1) consumption of first ammonia soln is the 1/3-3/5 of foliage fertilizer cumulative volume in; Step 3) speed that solution A joins in the solution B in is 60-120ml/min, and stirring velocity is 600-800rpm.
The anti-rising foliage fertilizer of the present invention's preparation has following advantage:
1, the anti-rising foliage fertilizer of the present invention's preparation provides comprehensive nutritive element for plant strain growth, the proportioning of each nutritive element is scientific and reasonable, solvability is good, anti-freezing cumulative power is strong, and effective constituent solubility and good dispersity are easy to plant absorbing, promote that the effect of plant growth is remarkable, the resistance of output increased, quality improving, plant and plant are obviously improved absorption and the running ability of trace element, effectively reduce the transpiration of plant, are applicable to arid biogeographic zone afforestation.
2, anti-rising foliage fertilizer of the present invention selects amine attitude nitrogen as the N source of plant-growth, is beneficial to plant leaf and absorbs, and directly the organism in plant materials is combined and is generated required amino acid, protein and other nitrogenous compound of plant-growth; Foliage fertilizer of the present invention has added the rare earth element of similar trace element, improves the chlorophyll content of plant, strengthens photosynthesis, promotes the growth of plant root, the growth of plant.
3, the anti-rising foliage fertilizer of the present invention's preparation has the adduction synergism to plant-growth, reduce on blade face pore opening degree and the blade face film forming and have dual adjusting function, reduce the multiple effects such as blade face moisture transpiration and enhancing blade face photosynthesis, drought-resistant ability with strengthening nutrition absorption.
4, use foliage fertilizer of the present invention and can increase the blade face stomatal resistance, reduce Transpiration, improve leaf water potential, strengthen the drought-resistant ability of plant, improve the surrival rate of afforestation of arid, semiarid zone.
5, anti-rising foliage fertilizer of the present invention can significantly promote plant leaf to increase, promote plant shoot growth amount, promotes the plant diameter accretion, promote plant total root quantity and root length to increase, and significantly improves the trophic level of plant.
6, anti-rising foliage fertilizer validity period of the present invention is long, nontoxic, harmless, pollution-free.
7, simple, the manufacturing condition of the preparation method of anti-rising foliage fertilizer of the present invention is controlled easily, and the process for preparation Raw adds successively and guaranteed the product solution homogeneous, and stability is high, does not produce precipitation, constant product quality, low production cost.
Description of drawings
Fig. 1 is locust tree stomatal resistance diurnal variation curve figure in July;
Fig. 2 is locust tree transpiration rate diurnal variation curve figure in July;
Fig. 3 is leaf of locust flow of water diurnal variation curve figure in July
Fig. 4 is locust tree number of blade change curve;
Fig. 5 is locust tree young sprout vary in diameter graphic representation;
Fig. 6 is locust tree young sprout height change graphic representation;
Fig. 7 is leaf of locust area change graphic representation.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage and disadvantage of the present invention will be more clear along with describing.But these embodiment only are exemplary, scope of the present invention are not consisted of any restriction.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can make amendment or replace the details of technical solution of the present invention and form, but these modifications and replacing all fall within the scope of protection of the present invention.
Embodiment 1
1, prepares raw material (preparation 100ml foliage fertilizer) according to following consumption
Copper sulfate 1g
Ethylenediamine tetraacetic acid (EDTA) 30g
Zinc sulfate 8g
Ferrous sulfate 2.6g
Manganous sulfate 2.5g
Ammonium molybdate 0.6g
Compound rare-earth 5g
Boric acid 8g
Sodium laurylsulfonate 2.5g
Urea 40g
Xanthohumic acid 3.5g
Diethylamino ethanol caproate 1g
Aminoacids complex 10.4g
Sal epsom 2g
Nitrocalcite 1.5g
Disodium ethylene diamine tetraacetate 5g
Potassium dihydrogen phosphate (mass percent concentration is 22.6%) 20ml
Microbiobacterial agent 10ml
Azone 2ml
Ammoniacal liquor (mass percent concentration 28%) is an amount of
Wherein, compound rare-earth is the farm rare-earth complex compound REAA that Henan Province Shangqiu City RE microbial fertilizer factory produces, the wherein total content of lanthanum trioxide, cerium oxide 〉=30%; Aminoacids complex is by amino-acid mixed being combined in following 17, aspartic acid, Isoleucine, Threonine, leucine, Serine, tyrosine, 4-L-glutamic acid, phenylalanine, glycine, Methionin, L-Ala, Histidine, Gelucystine, arginine, α-amino-isovaleric acid, proline(Pro), methionine(Met), wherein, the ratio of each amino acid whose weight part is followed successively by 5.2: 1.8: 1.9: 3.5: 2.0: 0.6: 0.5: 3.1: 2.5: 2.9: 2.5: 1.9: 0.8: 5.1: 0.6: 2.2: 2.9; Described microbiobacterial agent selects Beijing Mesosauridae skill company limited to produce gardenful spring microbiobacterial agent, living bacteria count 〉=400,000,000/ml wherein, organic content 60%, humic acids content 20%, effectively viable bacteria contains vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria, endophytic Bacillus, subtilis, Jingyang streptomycete, lactobacillus bulgaricus etc.
2, preparation solution A
Whole copper sulfate is joined in the 40ml ammonia soln, add successively in order again ethylenediamine tetraacetic acid (EDTA), potassium dihydrogen phosphate, zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, xanthohumic acid, sodium laurylsulfonate, urea, diethylamino ethanol caproate, aminoacids complex after heating, mixing, wherein add and be sequentially added into again another kind of raw material after a kind of raw material mixes, until add aminoacids complex and stir, make solution A, wherein the add-on of ammonia soln is 2/5 of foliage fertilizer cumulative volume; The temperature that mixes maintenance mixing solutions in the process is 45 ℃.
3, preparation solution B
Whole sal epsom is joined in the ammonia soln of 25ml, sequentially add disodium ethylene diamine tetraacetate again after heating, stirring, add again nitrocalcite after stirring, stir, make solution B, wherein, the add-on of ammonia soln is 1/4 of foliage fertilizer cumulative volume; The temperature that mixes maintenance mixing solutions in the process is 45 ℃.
4, preparation solution C
The solution A for preparing is slowly joined solution B, mix, make solution C, wherein the speed in the solution A adding solution B is 60-120ml/min, and stirring velocity is 600-800rpm.
5, the anti-rising foliage fertilizer of preparation
Whole azones, microbiobacterial agent are joined in the solution C successively, add again an amount of ammonia soln after stirring, stir and namely get the 100ml foliage fertilizer.
Embodiment 2
1, prepares raw material (preparation 100ml foliage fertilizer) according to following consumption
Copper sulfate 3g
Ethylenediamine tetraacetic acid (EDTA) 20g
Zinc sulfate 6g
Ferrous sulfate 3g
Manganous sulfate 2g
Ammonium molybdate 0.6g
Compound rare-earth 4g
Boric acid 8g
Sodium laurylsulfonate 2.0g
Urea 30g
Xanthohumic acid 3.5g
Diethylamino ethanol caproate 1.5g
Aminoacids complex 10.4g
Sal epsom 2g
Nitrocalcite 1g
Disodium ethylene diamine tetraacetate 4g
Potassium dihydrogen phosphate (mass percent concentration is 22.6%) 20ml
Microbiobacterial agent 10ml
Azone 3ml
Ammoniacal liquor (mass percent concentration is 25%) is an amount of
Wherein, compound rare-earth is the farm rare-earth complex compound REAA that Henan Province Shangqiu City RE microbial fertilizer factory produces, the wherein total content of lanthanum trioxide, cerium oxide 〉=30%; Aminoacids complex is by amino-acid mixed being combined in following 17, aspartic acid, Isoleucine, Threonine, leucine, Serine, tyrosine, 4-L-glutamic acid, phenylalanine, glycine, Methionin, L-Ala, Histidine, Gelucystine, arginine, α-amino-isovaleric acid, proline(Pro), methionine(Met), wherein, the ratio of each amino acid whose weight part is followed successively by 3.9: 4.2: 0.6: 2.2: 4.4: 1.8: 2.9: 1.8: 1.2: 5.3: 4.9: 0.6: 0.8: 7.5: 0.6: 0.9: 5.3; Described microbiobacterial agent selects Beijing Mesosauridae skill company limited to produce gardenful spring microbiobacterial agent, living bacteria count 〉=400,000,000/ml wherein, organic content 40%, humic acids content 10%, effectively viable bacteria contains vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria, endophytic Bacillus, subtilis, Jingyang streptomycete, lactobacillus bulgaricus etc.
2, preparation solution A
Whole copper sulfate is joined in the ammonia soln of 33.3ml, airtight, heating, add successively in order again ethylenediamine tetraacetic acid (EDTA) after mixing, potassium dihydrogen phosphate, zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, xanthohumic acid, sodium laurylsulfonate, urea, diethylamino ethanol caproate, aminoacids complex, wherein add and be sequentially added into again another kind of raw material after a kind of raw material mixes, until add aminoacids complex and stir, make solution A, wherein the add-on of ammonia soln is 1/3 of foliage fertilizer cumulative volume; The temperature that mixes maintenance mixing solutions in the process is 50 ℃.
3, preparation solution B
Whole sal epsom is joined in the ammonia soln of 16.7ml, airtight, heat, stir after again the order add disodium ethylene diamine tetraacetate, add again nitrocalcite after stirring, stir, make solution B, wherein, the add-on of ammonia soln is 1/6 of foliage fertilizer cumulative volume; The temperature that mixes maintenance mixing solutions in the process is 50 ℃.
4, preparation solution C
The solution A for preparing is slowly joined solution B, mix, make solution C.
5, the anti-rising foliage fertilizer of preparation
Whole azones, microbiobacterial agent are joined in the solution C successively, add again an amount of ammonia soln after stirring, stir and namely get the 100ml foliage fertilizer.
Embodiment 3
1, prepares raw material (preparation 100ml foliage fertilizer) according to following consumption
Copper sulfate 2g
Ethylenediamine tetraacetic acid (EDTA) 40g
Zinc sulfate 8g
Ferrous sulfate 2.6g
Manganous sulfate 2.5g
Ammonium molybdate 0.6g
Compound rare-earth 10g
Boric acid 10g
Sodium laurylsulfonate 2.5g
Urea 40g
Xanthohumic acid 3.5g
Diethylamino ethanol caproate 1g
Aminoacids complex 15g
Sal epsom 0g
Nitrocalcite 0g
Disodium ethylene diamine tetraacetate 0g
Potassium dihydrogen phosphate (mass percent concentration is 22.6%) 25ml
Microbiobacterial agent 2ml
Azone 0ml
Ammoniacal liquor (mass percent concentration is 28%) is an amount of
Wherein, compound rare-earth is the farm rare-earth complex compound REAA that Henan Province Shangqiu City RE microbial fertilizer factory produces, the wherein total content of lanthanum trioxide, cerium oxide 〉=30%; Aminoacids complex is by amino-acid mixed being combined in following 17, aspartic acid, Isoleucine, Threonine, leucine, Serine, tyrosine, 4-L-glutamic acid, phenylalanine, glycine, Methionin, L-Ala, Histidine, Gelucystine, arginine, α-amino-isovaleric acid, proline(Pro), methionine(Met), wherein, the ratio of each amino acid whose weight part is followed successively by 7.6: 0.5: 4.3: 5.9: 0.7: 0.6: 0.5: 3.7: 1.2: 1.6: 1.2: 2.5: 2.0: 3.8: 1.8: 2.0: 1.6; Described microbiobacterial agent selects Beijing Mesosauridae skill company limited to produce gardenful spring microbiobacterial agent, living bacteria count 〉=400,000,000/ml wherein, organic content 〉=40%, humic acids content 〉=10%, be rich in trace element in Calcium of intensifiens, magnesium, boron, zinc, the manganese etc., effectively viable bacteria mainly contains vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria, endophytic Bacillus, subtilis, Jingyang streptomycete, lactobacillus bulgaricus etc.
2, preparation solution A
Whole copper sulfate is joined in the ammonia soln of 40ml, airtight, heating, add successively in order again ethylenediamine tetraacetic acid (EDTA) after mixing, potassium dihydrogen phosphate, zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, xanthohumic acid, sodium laurylsulfonate, urea, diethylamino ethanol caproate, aminoacids complex, wherein add and be sequentially added into again another kind of raw material after a kind of raw material mixes, until add aminoacids complex and stir, make solution A, wherein the add-on of ammonia soln is 2/5 of foliage fertilizer cumulative volume; The temperature that mixes maintenance mixing solutions in the process is 50 ℃.
3, the anti-rising foliage fertilizer of preparation
Whole microbiobacterial agents is joined in the solution A successively, add again an amount of ammonia soln after stirring, stir and namely get the 100ml foliage fertilizer.
Embodiment 4
1, prepares raw material (preparation 100ml foliage fertilizer) according to following consumption
Copper sulfate 1g
Ethylenediamine tetraacetic acid (EDTA) 30g
Zinc sulfate 10g
Ferrous sulfate 2.6g
Manganous sulfate 2.5g
Ammonium molybdate 0.6g
Compound rare-earth 4g
Boric acid 7g
Sodium laurylsulfonate 3g
Urea 40g
Xanthohumic acid 4g
Diethylamino ethanol caproate 1g
Aminoacids complex 10.4g
Sal epsom 0g
Nitrocalcite 0g
Disodium ethylene diamine tetraacetate 0g
Potassium dihydrogen phosphate (mass percent concentration is 20%) 21ml
Microbiobacterial agent 2ml
Azone 0ml
Ammoniacal liquor (mass percent concentration is 28%) is an amount of
Wherein, compound rare-earth is the farm rare-earth complex compound REAA that Henan Province Shangqiu City RE microbial fertilizer factory produces, the wherein total content of lanthanum trioxide, cerium oxide 〉=30%; Aminoacids complex is by amino-acid mixed being combined in following 17, aspartic acid, Isoleucine, Threonine, leucine, Serine, tyrosine, 4-L-glutamic acid, phenylalanine, glycine, Methionin, L-Ala, Histidine, Gelucystine, arginine, α-amino-isovaleric acid, proline(Pro), methionine(Met), wherein, the ratio of each amino acid whose weight part is followed successively by 5.8: 1.8: 1.9: 3.5: 2.0: 3.0: 0.5: 5.5: 4.9: 2.9: 2.5: 1.9: 0.8: 5.1: 3.0: 4.6: 2.9; Described microbiobacterial agent selects Beijing Mesosauridae skill company limited to produce gardenful spring microbiobacterial agent, living bacteria count 〉=400,000,000/ml wherein, organic content 〉=40%, humic acids content 〉=10%, be rich in trace element in Calcium of intensifiens, magnesium, boron, zinc, the manganese etc., effectively viable bacteria contains vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria, endophytic Bacillus, subtilis, Jingyang streptomycete, lactobacillus bulgaricus etc.
2, preparation solution A
Whole copper sulfate is joined in the ammonia soln of 60ml, heating, add successively in order again ethylenediamine tetraacetic acid (EDTA) after mixing, potassium dihydrogen phosphate, zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, xanthohumic acid, sodium laurylsulfonate, urea, diethylamino ethanol caproate, aminoacids complex, wherein add and be sequentially added into again another kind of raw material after a kind of raw material stirring mixes, until add aminoacids complex and stir, make solution A, wherein the add-on of ammonia soln is 3/5 of foliage fertilizer cumulative volume; The temperature that mixes maintenance mixing solutions in the process is 60 ℃.
3, the anti-rising foliage fertilizer of preparation
Whole microbiobacterial agents is joined in the solution A successively, add again an amount of ammonia soln after stirring and namely get the 100ml foliage fertilizer.
Reference examples 1
With the special-purpose leaf nutritive agent of commercially available foliage fertilizer fruit tree (Beijing Dezhixin Science ﹠ Technology Development Co., Ltd.) example 1 in contrast.
Reference examples 2
With clear water in contrast 2.
The mensuration of test example 1 plant drought resistance index
1, experimental field with the test greenhouse
The test greenhouse is positioned at National forest park, Western Hills vulture peak, Beijing, and National forest park, vulture peak is located in the north, Taihang Mountain, Yanshan Mountain the east, and 39 ° 54 of north latitude ', 116 ° 28 of east longitude ', total area 811.73hm
2, National forest park, vulture peak cold summer heat, spring is windy, 12.2 ℃ of year-round average temperatures, mean wind speed 4.1m/s.Average annual precipitation 630mm focused mostly in 7～August, accounted for more than 70% of annual quantity of precipitation, and Winter-Spring precipitation accounts for about 10% of the whole year.Steam output is very large, and May is the highest, reaches 259mm.
The test greenhouse sits in the north facing the south and is the stainless steel frame structure, and take glass as main building material, light transmission is good.Outside the ventilation opening that the temperature removing chamber both sides have, towards greenhouse one side in south one row's window is installed and is beneficial to ventilate.
2, test materials
Choose essentially identical 2 years living Seedlings of Black Locusts of upgrowth situation mid-March, it is planted in upper bore is that 40cm, lower bore are in the plastic tub container of 30cm, high 35cm, adopt yellow cotton soil to be equipped with a small amount of soil ulmin as soil for growth, native about 90% in the basin of packing into, and compacting, make the unit weight of the soil in the basin near state of nature (about 1.20g/cm
3); Wherein, soil alkaline hydrolysis N content is 173.60mg/g, and available phosphorus contents is 61.97mg/g, and quick-acting potassium content is 299.67mg/g, and organic content is 32.07mg/g, and pH is 7.89, and specific conductivity is 0.40ms/cm; Water behind the seedling planting in good time, adopt Trime TDR determining soil moisture system (German IMKO company produce) to measure soil absolute water content, keeping soil absolute water content is 20%.
The foliage fertilizer of embodiment 1-4 preparation, the foliage fertilizer of reference examples 1 are added respectively 900 times of clear water dilutions, make the foliage fertilizer diluent, for subsequent use.
3, test method
The first tenday period of a month in May, Seedlings of Black Locust is grown when vigorous, the embodiment 1-4 of preparation, anti-rising foliage fertilizer diluent and reference examples 2 clear water of reference examples 1 are sprayed respectively equably at the tow sides of testing the nursery stock blade, treat to stop to spray when the blade face drop begins lower, spray per month 2 times, interval 15-16 days, fertilising began to survey the blade and blade flow of water and transpiration rate in rear 7-10 days.Each processes 15 strains, and 13 repetitions adopt completely random to put.
After the fertilising 7-10 days, select when fine, from processing, each gets 3 nursery stocks that individuality is close, and naked on nursery stock, breezy medium position is chosen the healthy Cheng Ye of phototropic face on a slice live body branch as the standard leaf; Every interval was measured the blade and blade flow of water, transpiration rate and stomatal resistance in two hours to point in afternoons 18 from 6 in the morning, processed each three data of record of measuring, taked same one week of mode METHOD FOR CONTINUOUS DETERMINATION for every group.
Adopt LI-1600 steady state porometer (production of U.S. LI-COR company) to measure transpiration rate and stomatal resistance, adopt Psypro dew point water potential meter (productions of U.S. Wescor company) mensuration leaf water potential, July locust tree stomatal resistance diurnal variation curve as shown in Figure 1; Locust tree transpiration rate daily variation in July as shown in Figure 2; Leaf of locust flow of water daily variation in July as shown in Figure 3.
The detected result of Fig. 1 shows: the daily variation of locust tree stomatal resistance is bimodal shape substantially, about 10 of mornings reach first stomatal resistance peak, and 14 stomatal resistances reach large peak in the afternoon, and this stomatal properties that shows locust tree to external world condition changes more responsive.The processing stomatal resistance that sprays anti-rising foliage fertilizer after at 16 in afternoon obviously weakens, and substantially maintains an equal level with contrast; The stomatal resistance mean value that sprays novel anti-rising foliage fertilizer rear blade of the present invention all is higher than reference examples.Illustrate that this anti-rising foliage fertilizer effect aspect the effective transpiration of raising plant is remarkable.
The detected result of Fig. 2 shows: transpiration rate changes and is " bimodal " in the locust tree one day, appears at respectively about 16 of 12 noon and afternoons, and wherein take 12 noon as large peak, and at 16 in afternoon is little peak; After BLACK LOCUST SEEDLINGS sprayed foliage fertilizer, the transpiration rate contrast mean value of blade reduced by 43.1%～78.6%.
The detected result of Fig. 3 shows: day in the July process of the leaf of locust flow of water is " W " shape substantially.Sooner or later the flow of water is in the middle high level of whole day.In at 10 in the morning decline is arranged, after this bottom out is until 12 noon.14 left and right sides flow of water drop in afternoon, its day process larger wave amplitude [Δ ∈ (0.12Mpa～0.33Mpa)] is all arranged; Foliage fertilizer of the present invention can significantly improve the locust tree water potential value, and the delay plant dehydration effect is strong.
The mensuration of test example 2 plant-growth indexs
The observation time of plant-growth index is 5-10 month, and the time of at every turn measuring plant leaf, young sprout height, young sprout diameter, phytomass is chosen at carries out the day before yesterday that sprays foliage fertilizer.
1, the mensuration of plant leaf, young sprout height, young sprout diameter
Utilize respectively tape measure, electronic cursor slide calliper rule to measure shoot growth height and the young sprout diameter of Seedlings of Black Locust, and plant leaf, young sprout growth are added up, locust tree number of blade change curve as shown in Figure 4; Locust tree young sprout vary in diameter curve as shown in Figure 5; Locust tree young sprout height change curve as shown in Figure 6.Leaf of locust Area Growth curve as shown in Figure 7.
The measurement result of Fig. 4 shows: locust tree is at 5-8 number of blade rapid growth between the month, and the number of blade increases and reaches maximum amplitude and reach the peak period of the number of blade at 8 the end of month at the beginning of 8 months, and September, the reduction number of blade along with temperature began to reduce; The number of blade of anti-rising foliage fertilizer of the present invention illustrates that obviously more than reference examples foliage fertilizer of the present invention is promoting to have clear superiority aspect the plant leaf growth.
The measurement result of Fig. 5 shows: locust tree young sprout diameter slowly increases after spraying foliage fertilizer, increase substantially S-shaped, spray foliage fertilizer of the present invention the plant shoot growth is had significant promoter action, when Growth of Blaek Locust ran into the extreme environment at 8 the end of month, foliage fertilizer had also played the effect of raising stress resistance of plant.
The measurement result of Fig. 6 shows: locust tree is the para-curve ascending-type substantially in the growth of 6-9 young sprout height between the month, reaches the highest after September; Locust tree shoot growth height is obviously greater than reference examples shoot growth height after spraying foliage fertilizer of the present invention.
The measurement result of Fig. 7 shows: compare with reference examples, foliage fertilizer of the present invention can significantly be accelerated the growth of blade area.
In a word, the stomatal resistance on locust tree blade face increases behind the anti-rising foliage fertilizer of use the present invention, leaf water potential increases, transpiration weakens, and anti-rising effect is remarkable.
2, the mensuration of plant total biomass
At first at the beginning of 10 months with all over-ground parts of Seedlings of Black Locust of variant processing along under the ground surface harvesting, after weighing, in the plastic sealing bag of getting ready in advance of packing into; Whole tree root is poured out from plastic tub, water flushing, flotation, dry, collect, weigh and the sealing bag of packing in for subsequent use;
Then place 85 ℃ convection oven to dry 30min all parts of plant, then sample is packed in the cloth bag, in 65 ℃ convection oven, dry 12h again, then the gross dry weight of plant behind the weighing drying and processing, be converted into total biomass, total biomass measurement result such as table 1.
Table 1 locust tree total biomass measurement result
| The upperground part biomass (g/ strain) | Underground part biomass (g/ strain) | |
| Embodiment 1 | 134.26 | 48.29 |
| Embodiment 2 | 93.26 | 29.42 |
| Embodiment 3 | 101.31 | 30.51 |
| Embodiment 4 | 78.225 | 24.77 |
| Reference examples 1 | 76.08 | 23.49 |
| Reference examples 2 | 70.225 | 18.96 |
The mensuration of nutritive index in test example 3 plant leafs
1, the mensuration of N, P, K element in the plant leaf
In the sample that 13 strains of each processing repeat, the herborization on the ground ripe and blade of uniform size in position with rapidly flushing 3 times of tap water, is used distilled water flushing 2 times as sample again, dries, and dries to constant weight under 70 ℃; The sample of oven dry is pulverized with lapping apparatus, crossed 60 mesh sieves, and mix, put into sampler bag and save backup.
Utilize elemental analyser (Vario EL III, CHNOS Elemental Analyzer, Elementar CoGermany) to measure plant N constituent content; Utilize gas ions jigger coupling emission spectrometer (ICP-OES, Optima2000DC, Perkinv Elmer Co U.S.A) to measure respectively the concentration of the elements such as P, K of plant, measurement result such as table 2.
N, P, K assay result in the table 2 locust tree blade
| N content (mg/g) | P content (mg/g) | K content (mg/g) | |
| Embodiment 1 | 4.695 | 2.225 | 18.918 |
| Embodiment 2 | 3.587 | 2.174 | 18.725 |
| Embodiment 3 | 4.051 | 1.798 | 15.318 |
| Embodiment 4 | 3.193 | 1.993 | 17.377 |
| Reference examples 1 | 3.269 | 1.675 | 13.152 |
| Reference examples 2 | 2.685 | 1.404 | 12.215 |
Test-results shows: the foliage fertilizer of the present invention's preparation can effectively promote locust tree to the absorption of N, P, K element, and the content of N, P, K element is high in the locust tree blade.Compare with reference examples 2 with reference examples 1, spray this foliage fertilizer after, main nutrient elements (N, P, the K) increased content that plant is required.
Claims (6)
1. the preparation method of the anti-rising foliage fertilizer of liquid state comprises the steps:
1) component that contains following proportioning according to every 100ml foliage fertilizer is prepared raw material
2) whole copper sulfate, ethylenediamine tetraacetic acid (EDTA), zinc sulfate, ferrous sulfate, manganous sulfate, ammonium molybdate, compound rare-earth, boric acid, xanthohumic acid, sodium laurylsulfonate, urea, diethylamino ethanol caproate, aminoacids complex, potassium dihydrogen phosphate are joined in first ammonia soln successively, be heated to 45-60 ℃, be to mix under 45-60 ℃ in temperature, make solution A, wherein the consumption of first ammonia soln is the 1/5-3/5 of foliage fertilizer cumulative volume;
3) whole sal epsom, disodium ethylene diamine tetraacetate, nitrocalcite are joined in the second batch ammonia soln successively, the temperature of heating and maintenance mixing solutions is 45-60 ℃, stir, make solution B, wherein the consumption of second batch ammonia soln is the 1/6-1/4 of foliage fertilizer cumulative volume;
4) solution A for preparing is joined solution B, mix, make solution C;
5) add successively the ammonia soln of whole azones and microbiobacterial agent and surplus in the solution C that makes, stir and get final product.
2. method as claimed in claim 1, the mass percent concentration that it is characterized in that described ammonia soln is 25-28%.
3. method as claimed in claim 1 or 2 is characterized in that the total content of lanthanum trioxide, cerium oxide in the described compound rare-earth 〉=30%.
4. method as claimed in claim 1 or 2 is characterized in that viable count in the described microbiobacterial agent 〉=400,000,000/ml, organic mass percent concentration 〉=40%, the mass percent concentration of humic acids 〉=10%.
5. method as claimed in claim 1 or 2 is characterized in that described aminoacids complex by aspartic acid, Isoleucine, Threonine, leucine, Serine, tyrosine, 4-L-glutamic acid, phenylalanine, glycine, Methionin, L-Ala, Histidine, Gelucystine, arginine, α-amino-isovaleric acid, proline(Pro), methionine(Met) forms.
6. method as claimed in claim 1 or 2, the mass percent concentration that it is characterized in that described potassium dihydrogen phosphate is 20-22.6%.
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