CN101903529A - 基于sm-蛋白的分泌工程化 - Google Patents

基于sm-蛋白的分泌工程化 Download PDF

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Publication number
CN101903529A
CN101903529A CN2008801221856A CN200880122185A CN101903529A CN 101903529 A CN101903529 A CN 101903529A CN 2008801221856 A CN2008801221856 A CN 2008801221856A CN 200880122185 A CN200880122185 A CN 200880122185A CN 101903529 A CN101903529 A CN 101903529A
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China
Prior art keywords
protein
cell
expression
albumen
seq
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Pending
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CN2008801221856A
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English (en)
Chinese (zh)
Inventor
希托·考夫曼
埃里克·贝克
劳尔·弗洛林
马丁·弗森尼格
彭仁旺
乔伊·M·斯图茨
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Boehringer Ingelheim Pharma GmbH and Co KG
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Boehringer Ingelheim Pharma GmbH and Co KG
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Application filed by Boehringer Ingelheim Pharma GmbH and Co KG filed Critical Boehringer Ingelheim Pharma GmbH and Co KG
Publication of CN101903529A publication Critical patent/CN101903529A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008801221856A 2007-12-20 2008-12-19 基于sm-蛋白的分泌工程化 Pending CN101903529A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP07150254 2007-12-20
EP07150254.6 2007-12-20
EP08152829 2008-03-17
EP08152829.1 2008-03-17
PCT/EP2008/010882 WO2009080299A1 (en) 2007-12-20 2008-12-19 Sm-protein based secretion engineering

Publications (1)

Publication Number Publication Date
CN101903529A true CN101903529A (zh) 2010-12-01

Family

ID=40344722

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008801221856A Pending CN101903529A (zh) 2007-12-20 2008-12-19 基于sm-蛋白的分泌工程化

Country Status (14)

Country Link
US (1) US20090247609A1 (enExample)
EP (1) EP2225389A1 (enExample)
JP (1) JP2011505850A (enExample)
KR (1) KR20100099190A (enExample)
CN (1) CN101903529A (enExample)
AR (1) AR069958A1 (enExample)
AU (1) AU2008340652A1 (enExample)
BR (1) BRPI0821389A2 (enExample)
CA (1) CA2709645A1 (enExample)
EA (1) EA201000945A1 (enExample)
IL (1) IL205239A0 (enExample)
NZ (1) NZ586037A (enExample)
TW (1) TW200932907A (enExample)
WO (1) WO2009080299A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106554973A (zh) * 2015-09-30 2017-04-05 北京吉尚立德生物科技有限公司 一种cho细胞分泌能力评价系统
CN110835624A (zh) * 2012-05-29 2020-02-25 瑞泽恩制药公司 生产型细胞系的增强子
WO2023155881A1 (en) * 2022-02-18 2023-08-24 Tsinghua University Methods for regulating secretion via migrasomes

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011033375A2 (en) 2009-09-18 2011-03-24 Selexis S.A. Products and methods for enhanced transgene expression and processing
PL2951309T3 (pl) 2013-02-01 2019-06-28 Selexis S.A. Wzmocniona ekspresja transgenu i przetwarzanie
ES2837699T3 (es) 2014-12-19 2021-07-01 Merz Pharma Gmbh & Co Kgaa Medios y métodos para la determinación de la actividad biológica de BoNT/E en células
US20230051925A1 (en) * 2020-01-20 2023-02-16 Ev Cell Biotech (Guangzhou) Co., Ltd. Vesicle and use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0665890B1 (en) * 1992-10-06 2003-11-19 Valtion Teknillinen Tutkimuskeskus Increased production of secreted proteins by recombinant eukaryotic cells
WO2004042052A1 (en) * 2002-11-08 2004-05-21 The University Of Queensland Modulating tnf (alpha) secretion

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999007862A1 (en) * 1997-08-05 1999-02-18 Chiron Corporation Novel pichia pastoris gene sequences and methods for their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0665890B1 (en) * 1992-10-06 2003-11-19 Valtion Teknillinen Tutkimuskeskus Increased production of secreted proteins by recombinant eukaryotic cells
WO2004042052A1 (en) * 2002-11-08 2004-05-21 The University Of Queensland Modulating tnf (alpha) secretion

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EUNJIN OH, ET AL: "WNK1 is a novel regulator of Munc18c-syntaxin 4 complex formation in soluble NSF attachment protein receptor (SNARE)-mediated vesicle exocytosis", 《THE JOURNAL OF BIOLOGICAL CHEMISTRY》 *
RUUD F.G. TOONEN ET AL: "Vesicle trafficking:pleasure and pain from SM genes", 《TRENDS IN CELL BIOLOGY》 *
TODD D SCHRAW ET AL: "A role for Sec1/Munc18 proteins in platelet exocytosis", 《BIOCHEMICAL JOURNAL》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110835624A (zh) * 2012-05-29 2020-02-25 瑞泽恩制药公司 生产型细胞系的增强子
CN106554973A (zh) * 2015-09-30 2017-04-05 北京吉尚立德生物科技有限公司 一种cho细胞分泌能力评价系统
CN106554973B (zh) * 2015-09-30 2020-05-22 北京吉尚立德生物科技有限公司 一种cho细胞分泌能力评价系统
WO2023155881A1 (en) * 2022-02-18 2023-08-24 Tsinghua University Methods for regulating secretion via migrasomes

Also Published As

Publication number Publication date
EP2225389A1 (en) 2010-09-08
US20090247609A1 (en) 2009-10-01
KR20100099190A (ko) 2010-09-10
JP2011505850A (ja) 2011-03-03
BRPI0821389A2 (pt) 2015-06-16
WO2009080299A8 (en) 2010-04-15
IL205239A0 (en) 2010-11-30
NZ586037A (en) 2012-08-31
TW200932907A (en) 2009-08-01
EA201000945A1 (ru) 2011-02-28
CA2709645A1 (en) 2009-07-02
AR069958A1 (es) 2010-03-03
WO2009080299A1 (en) 2009-07-02
AU2008340652A1 (en) 2009-07-02

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