CN101889096B - 核酸扩增 - Google Patents

核酸扩增 Download PDF

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Publication number
CN101889096B
CN101889096B CN200880119335.8A CN200880119335A CN101889096B CN 101889096 B CN101889096 B CN 101889096B CN 200880119335 A CN200880119335 A CN 200880119335A CN 101889096 B CN101889096 B CN 101889096B
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CN
China
Prior art keywords
primer
oligonucleotide
sequence
helper
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN200880119335.8A
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English (en)
Chinese (zh)
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CN101889096A (zh
Inventor
基思·N·兰德
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Priority claimed from AU2007905445A external-priority patent/AU2007905445A0/en
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Publication of CN101889096A publication Critical patent/CN101889096A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880119335.8A 2007-10-04 2008-10-03 核酸扩增 Expired - Fee Related CN101889096B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2007905445A AU2007905445A0 (en) 2007-10-04 Nucleic acid amplification
AU2007905445 2007-10-04
PCT/AU2008/001475 WO2009043112A1 (en) 2007-10-04 2008-10-03 Nucleic acid amplification

Publications (2)

Publication Number Publication Date
CN101889096A CN101889096A (zh) 2010-11-17
CN101889096B true CN101889096B (zh) 2015-10-21

Family

ID=40525777

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880119335.8A Expired - Fee Related CN101889096B (zh) 2007-10-04 2008-10-03 核酸扩增

Country Status (7)

Country Link
US (1) US8455197B2 (cg-RX-API-DMAC7.html)
EP (1) EP2207896B1 (cg-RX-API-DMAC7.html)
JP (1) JP5535921B2 (cg-RX-API-DMAC7.html)
CN (1) CN101889096B (cg-RX-API-DMAC7.html)
AU (1) AU2008307153B2 (cg-RX-API-DMAC7.html)
ES (1) ES2460896T3 (cg-RX-API-DMAC7.html)
WO (1) WO2009043112A1 (cg-RX-API-DMAC7.html)

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US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2011009073A1 (en) * 2009-07-16 2011-01-20 Abbott Laboratories A method for amplification of nucleic acids
DK2516680T3 (en) 2009-12-22 2016-05-02 Sequenom Inc Method and kits to identify aneuploidy
EP2702168B1 (en) 2011-04-29 2018-01-17 Sequenom, Inc. Quantification of a minority nucleic acid species
AU2012340118A1 (en) * 2011-11-17 2014-04-24 Rheonix, Inc. System and methods for selective molecular analysis
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK3594366T3 (da) 2012-05-11 2021-09-13 Clinical Genomics Pty Ltd Diagnostisk genmarkørpanel
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
HK1206792A1 (en) 2012-07-13 2016-01-15 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CN104822840A (zh) * 2012-12-13 2015-08-05 合成基因组股份有限公司 Peg-介导的核酸分子的组装
EP3597774A1 (en) 2013-03-13 2020-01-22 Sequenom, Inc. Primers for dna methylation analysis
WO2014145176A1 (en) * 2013-03-15 2014-09-18 Arnold Lyle J Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence
CN113832216A (zh) * 2013-03-15 2021-12-24 莱尔·J·阿诺德 使用发卡寡核苷酸扩增核酸的方法
EP4321867B1 (en) * 2013-08-09 2025-10-01 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2015138774A1 (en) 2014-03-13 2015-09-17 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2861353T3 (es) * 2014-05-19 2021-10-06 Univ Rice William M Amplificación específica de alelo utilizando una composición de oligonucleótidos cebadores no específicos de alelo y bloqueantes específicos de alelo solapantes
CN104131070A (zh) * 2014-05-22 2014-11-05 苏州工业园区为真生物医药科技有限公司 一种对基因甲基化检测的方法
EP3209801B1 (en) 2014-10-20 2023-06-28 Commonwealth Scientific and Industrial Research Organisation Genome methylation analysis
CN105331727B (zh) * 2015-12-01 2019-09-03 湖南宏雅基因技术有限公司 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
WO2018024562A1 (en) * 2016-08-02 2018-02-08 Roche Diagnostics Gmbh Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
JP7001678B2 (ja) 2016-09-06 2022-02-04 スウィフト バイオサイエンシーズ， インコーポレイテッド Ｎｇｓライブラリー濃度の正規化
DE102016120124B8 (de) * 2016-10-21 2018-08-23 Gna Biosolutions Gmbh Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens
US10787699B2 (en) * 2017-02-08 2020-09-29 Microsoft Technology Licensing, Llc Generating pluralities of primer and payload designs for retrieval of stored nucleotides
WO2019162529A1 (de) * 2018-02-26 2019-08-29 Agct Gmbh Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
EP3530753A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
EP3530754A1 (de) * 2018-02-26 2019-08-28 AGCT GmbH Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität
CN112074612A (zh) * 2018-02-26 2020-12-11 Agct有限公司 一种具有更高特异性的核酸扩增方法
US20210071244A1 (en) * 2018-02-28 2021-03-11 Agct Gmbh Method for the amplification of a nucleic acid with improved specificity
CN114075595B (zh) * 2021-11-22 2023-11-17 上海交通大学 甲基化检测组合物、试剂盒及方法

Citations (2)

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Publication number Priority date Publication date Assignee Title
US6335184B1 (en) * 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids

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US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
KR20040028991A (ko) * 2001-08-20 2004-04-03 다카라 바이오 가부시키가이샤 핵산 증폭 방법
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US20060014183A1 (en) * 2004-06-10 2006-01-19 Pfundheller Henrik M Extendable probes
US8501403B2 (en) * 2006-03-28 2013-08-06 Commonwealth Scientific And Industrial Research Organisation Amplification of DNA fragments
WO2008064687A1 (en) * 2006-11-27 2008-06-05 Fluimedix Fidelity enhanced allele specific amplification method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) * 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids

Also Published As

Publication number Publication date
EP2207896A1 (en) 2010-07-21
JP5535921B2 (ja) 2014-07-02
WO2009043112A1 (en) 2009-04-09
EP2207896B1 (en) 2014-01-29
JP2010539956A (ja) 2010-12-24
ES2460896T3 (es) 2014-05-14
CN101889096A (zh) 2010-11-17
AU2008307153B2 (en) 2015-03-12
US20110033851A1 (en) 2011-02-10
EP2207896A4 (en) 2010-11-10
AU2008307153A1 (en) 2009-04-09
US8455197B2 (en) 2013-06-04

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