CN101835898B - 用于生物活性肽表达和纯化的可溶性标记 - Google Patents

用于生物活性肽表达和纯化的可溶性标记 Download PDF

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Publication number
CN101835898B
CN101835898B CN2008800253760A CN200880025376A CN101835898B CN 101835898 B CN101835898 B CN 101835898B CN 2008800253760 A CN2008800253760 A CN 2008800253760A CN 200880025376 A CN200880025376 A CN 200880025376A CN 101835898 B CN101835898 B CN 101835898B
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peptide
gln
seq
pro
gly
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Chinese (zh)
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CN101835898A (zh
Inventor
Q·程
L·J·德卡罗利斯
S·R·法内斯托克
T·M·格鲁伯
L·D·里斯
P·E·鲁维埃
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2008800253760A 2007-07-25 2008-07-23 用于生物活性肽表达和纯化的可溶性标记 Expired - Fee Related CN101835898B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/782,836 2007-07-25
US11/782,836 US7678883B2 (en) 2007-07-25 2007-07-25 Solubility tags for the expression and purification of bioactive peptides
PCT/US2008/070802 WO2009015165A1 (en) 2007-07-25 2008-07-23 Solubility tags for the expression and purification of bioactive peptides

Publications (2)

Publication Number Publication Date
CN101835898A CN101835898A (zh) 2010-09-15
CN101835898B true CN101835898B (zh) 2013-01-02

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CN2008800253760A Expired - Fee Related CN101835898B (zh) 2007-07-25 2008-07-23 用于生物活性肽表达和纯化的可溶性标记

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Country Link
US (2) US7678883B2 (https=)
EP (1) EP2173877B1 (https=)
JP (1) JP5453261B2 (https=)
KR (1) KR20100057016A (https=)
CN (1) CN101835898B (https=)
AU (1) AU2008279186B2 (https=)
CA (1) CA2689099A1 (https=)
ES (1) ES2439702T3 (https=)
IL (1) IL203002A0 (https=)
WO (1) WO2009015165A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI567197B (zh) * 2014-12-29 2017-01-21 財團法人農業科技研究院 表現元件、表現卡匣、及含其之載體
CN109477113A (zh) * 2015-07-24 2019-03-15 爱沃克斯技术有限责任公司 催化活性蛋白质聚集体及其制备方法

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US9101687B2 (en) 2002-07-15 2015-08-11 University Of Leeds Beta sheet tapes ribbons in tissue engineering
US8586539B2 (en) 2002-07-15 2013-11-19 University Of Leeds Beta sheet tapes ribbons in tissue engineering
US7951559B2 (en) * 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
US7829311B2 (en) * 2007-07-25 2010-11-09 E.I. Du Pont De Nemours And Company Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate
US7915011B2 (en) * 2009-03-05 2011-03-29 E.I. Du Pont De Nemours And Company Host cell modifications that improve peptide production and downstream processing
US9241987B2 (en) 2009-11-20 2016-01-26 The University Of Chicago Methods and compositions related to immunogenic fibrils
US8609621B2 (en) 2010-11-15 2013-12-17 E I Du Pont De Nemours And Company Acid-cleavable linkers exhibiting altered rates of acid hydrolysis
US9273336B2 (en) 2011-02-21 2016-03-01 E. I. Du Pont De Nemours And Company Recombinant host cells having an increase in buoyant density
US9115182B2 (en) * 2011-06-21 2015-08-25 E I Du Pont De Nemours And Company Cysteine cross-linked structural peptides
US9062312B2 (en) * 2011-06-21 2015-06-23 Danisco Us Inc. Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications
WO2014056199A1 (zh) * 2012-10-12 2014-04-17 清华大学 多肽的产生和纯化方法
GB201306338D0 (en) 2013-04-08 2013-05-22 Univ Leeds Novel proteoglycans
CN104513298B (zh) * 2014-12-09 2018-07-17 山东信得科技股份有限公司 一种表达鸡传染性法氏囊可溶性vp 2-4-3多肽的方法
JP7623006B2 (ja) * 2018-09-11 2025-01-28 ナノタグ バイオテクノロジーズ ゲーエムベーハー 特異的結合剤により認識されるエピトープタグ
US20250258180A1 (en) * 2021-07-27 2025-08-14 Stand Therapeutics Co., Ltd. Peptide tag and nucleic acid encoding same
US20250042946A1 (en) 2021-12-08 2025-02-06 Oakgrove Bio Llc Biological Production of Histidine-Rich Peptides
EP4567108A1 (en) * 2023-12-05 2025-06-11 Kutzner, Christoph Screening of target peptides using fusion polypeptides

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI567197B (zh) * 2014-12-29 2017-01-21 財團法人農業科技研究院 表現元件、表現卡匣、及含其之載體
CN109477113A (zh) * 2015-07-24 2019-03-15 爱沃克斯技术有限责任公司 催化活性蛋白质聚集体及其制备方法

Also Published As

Publication number Publication date
CN101835898A (zh) 2010-09-15
CA2689099A1 (en) 2009-01-29
JP5453261B2 (ja) 2014-03-26
JP2010534477A (ja) 2010-11-11
EP2173877A1 (en) 2010-04-14
EP2173877B1 (en) 2013-10-02
WO2009015165A1 (en) 2009-01-29
AU2008279186A1 (en) 2009-01-29
AU2008279186B2 (en) 2013-09-05
ES2439702T3 (es) 2014-01-24
US7678883B2 (en) 2010-03-16
US20090029412A1 (en) 2009-01-29
IL203002A0 (en) 2011-08-01
US7794979B2 (en) 2010-09-14
US20100136621A1 (en) 2010-06-03
KR20100057016A (ko) 2010-05-28

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