KR20100057016A - 생활성 펩티드의 발현 및 정제를 위한 가용성 태그 - Google Patents

생활성 펩티드의 발현 및 정제를 위한 가용성 태그 Download PDF

Info

Publication number
KR20100057016A
KR20100057016A KR1020107004040A KR20107004040A KR20100057016A KR 20100057016 A KR20100057016 A KR 20100057016A KR 1020107004040 A KR1020107004040 A KR 1020107004040A KR 20107004040 A KR20107004040 A KR 20107004040A KR 20100057016 A KR20100057016 A KR 20100057016A
Authority
KR
South Korea
Prior art keywords
peptide
gln
pro
gly
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020107004040A
Other languages
English (en)
Korean (ko)
Inventor
키옹 쳉
린다 제인 드캐롤리스
스티븐 알. 파네스톡
타냐 마리아 그루버
리사 다이안 라이쓰
피에르 이. 루비에르
Original Assignee
이 아이 듀폰 디 네모아 앤드 캄파니
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 이 아이 듀폰 디 네모아 앤드 캄파니 filed Critical 이 아이 듀폰 디 네모아 앤드 캄파니
Publication of KR20100057016A publication Critical patent/KR20100057016A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020107004040A 2007-07-25 2008-07-23 생활성 펩티드의 발현 및 정제를 위한 가용성 태그 Ceased KR20100057016A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/782,836 2007-07-25
US11/782,836 US7678883B2 (en) 2007-07-25 2007-07-25 Solubility tags for the expression and purification of bioactive peptides

Publications (1)

Publication Number Publication Date
KR20100057016A true KR20100057016A (ko) 2010-05-28

Family

ID=39967949

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020107004040A Ceased KR20100057016A (ko) 2007-07-25 2008-07-23 생활성 펩티드의 발현 및 정제를 위한 가용성 태그

Country Status (10)

Country Link
US (2) US7678883B2 (https=)
EP (1) EP2173877B1 (https=)
JP (1) JP5453261B2 (https=)
KR (1) KR20100057016A (https=)
CN (1) CN101835898B (https=)
AU (1) AU2008279186B2 (https=)
CA (1) CA2689099A1 (https=)
ES (1) ES2439702T3 (https=)
IL (1) IL203002A0 (https=)
WO (1) WO2009015165A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9101687B2 (en) 2002-07-15 2015-08-11 University Of Leeds Beta sheet tapes ribbons in tissue engineering
US8586539B2 (en) 2002-07-15 2013-11-19 University Of Leeds Beta sheet tapes ribbons in tissue engineering
US7951559B2 (en) * 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
US7829311B2 (en) * 2007-07-25 2010-11-09 E.I. Du Pont De Nemours And Company Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate
US7915011B2 (en) * 2009-03-05 2011-03-29 E.I. Du Pont De Nemours And Company Host cell modifications that improve peptide production and downstream processing
US9241987B2 (en) 2009-11-20 2016-01-26 The University Of Chicago Methods and compositions related to immunogenic fibrils
US8609621B2 (en) 2010-11-15 2013-12-17 E I Du Pont De Nemours And Company Acid-cleavable linkers exhibiting altered rates of acid hydrolysis
US9273336B2 (en) 2011-02-21 2016-03-01 E. I. Du Pont De Nemours And Company Recombinant host cells having an increase in buoyant density
US9115182B2 (en) * 2011-06-21 2015-08-25 E I Du Pont De Nemours And Company Cysteine cross-linked structural peptides
US9062312B2 (en) * 2011-06-21 2015-06-23 Danisco Us Inc. Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications
WO2014056199A1 (zh) * 2012-10-12 2014-04-17 清华大学 多肽的产生和纯化方法
GB201306338D0 (en) 2013-04-08 2013-05-22 Univ Leeds Novel proteoglycans
CN104513298B (zh) * 2014-12-09 2018-07-17 山东信得科技股份有限公司 一种表达鸡传染性法氏囊可溶性vp 2-4-3多肽的方法
JP6640226B2 (ja) * 2014-12-29 2020-02-05 アグリカルチュラル テクノロジー リサーチ インスティテュート 発現エレメント、発現カセット、及びそれらを含むベクター
GB2540763A (en) * 2015-07-24 2017-02-01 Evocatal Gmbh Catalytically active protein aggregates and methods for producing the same
JP7623006B2 (ja) * 2018-09-11 2025-01-28 ナノタグ バイオテクノロジーズ ゲーエムベーハー 特異的結合剤により認識されるエピトープタグ
US20250258180A1 (en) * 2021-07-27 2025-08-14 Stand Therapeutics Co., Ltd. Peptide tag and nucleic acid encoding same
US20250042946A1 (en) 2021-12-08 2025-02-06 Oakgrove Bio Llc Biological Production of Histidine-Rich Peptides
EP4567108A1 (en) * 2023-12-05 2025-06-11 Kutzner, Christoph Screening of target peptides using fusion polypeptides

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US516362A (en) * 1894-03-13 Retarding device for bolt-actuating mechanism
US641273A (en) * 1899-06-03 1900-01-16 John Dennis Coon Cooking utensil.
US641936A (en) * 1899-08-09 1900-01-23 Edwin R Childs Latticed or leaded glass.
US935642A (en) * 1908-01-09 1909-10-05 Gerhard Coenen Flushing apparatus.
US5206154A (en) * 1985-01-28 1993-04-27 Xoma Corporation Method of producing cecropins by microbiological techniques
WO1988007086A1 (en) * 1987-03-20 1988-09-22 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
AU638762B2 (en) * 1989-10-05 1993-07-08 Optein Inc Cell-free synthesis and isolation of novel genes and polypeptides
US5449754A (en) * 1991-08-07 1995-09-12 H & N Instruments, Inc. Generation of combinatorial libraries
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
ATE233817T1 (de) 1992-08-21 2003-03-15 Univ British Columbia Kationische peptide und verfahren zu deren herstellung
US5585275A (en) * 1992-09-02 1996-12-17 Arris Pharmaceutical Corporation Pilot apparatus for peptide synthesis and screening
US5480971A (en) * 1993-06-17 1996-01-02 Houghten Pharmaceuticals, Inc. Peralkylated oligopeptide mixtures
US5648244A (en) * 1993-09-27 1997-07-15 President And Fellows Of Harvard College Production, purification, cleavage and use of fusion peptides
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
ATE332368T1 (de) * 1997-01-21 2006-07-15 Gen Hospital Corp Selektion von proteinen mittels rns-protein fusionen
US6261804B1 (en) * 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
US6281018B1 (en) * 1998-02-26 2001-08-28 Coulter International Corp. Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions
US6846655B1 (en) * 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
US6613548B1 (en) * 1998-07-31 2003-09-02 Pierce Biotechnology, Inc. Fusion products containing insoluble proteinaceous tag
JP2002522091A (ja) * 1998-08-17 2002-07-23 フィロス インク. 3’非翻訳領域を欠損する核酸を生成して細胞rna蛋白質融合体形成を最適化する方法
JP4278872B2 (ja) * 1998-08-17 2009-06-17 フィロス インク. 蛋白質・核酸融合分子ライブラリーを用いた化合物・蛋白質相互作用の同定
US6696089B2 (en) 1998-09-03 2004-02-24 Board Of Regents Of The University Of Nebraska Nanogel networks including polyion polymer fragments and biological agent compositions thereof
FR2783169B1 (fr) * 1998-09-15 2001-11-02 Sederma Sa Utilisation cosmetique ou dermopharmaceutique de peptides pour la cicatrisation et pour l'amelioration de l'aspect cutane lors du vieillissement naturel ou accelere (heliodermie, pollution)
NZ511699A (en) * 1998-12-02 2003-02-28 Phylos Inc DNA-protein fusions and uses thereof
US6242219B1 (en) * 1999-03-18 2001-06-05 Xoma (Us) Llc Methods for recombinant peptide production
DK1196637T3 (da) * 1999-07-27 2007-07-30 Adnexus Therapeutics Inc Peptidacceptor-ligeringsmetoder
US6436665B1 (en) * 1999-08-27 2002-08-20 Phylos, Inc Methods for encoding and sorting in vitro translated proteins
CA2402452A1 (en) * 2000-03-31 2001-10-11 Cambridge Antibody Technology Limited Improvements to ribosome display
US20020098524A1 (en) 2000-04-14 2002-07-25 Murray Christopher J. Methods for selective targeting
US7129326B2 (en) 2000-04-14 2006-10-31 Genencor International, Inc. Methods for selective targeting
GB0117011D0 (en) * 2001-07-12 2001-09-05 Univ Leeds Peptide barrels
AU2002367566B2 (en) * 2001-11-20 2008-10-16 Duke University Interfacial biomaterials
CA2485835C (en) * 2002-05-24 2018-03-06 Restoragen, Inc. Methods and dna constructs for high yield production of polypeptides
WO2003100022A2 (en) * 2002-05-24 2003-12-04 Restoragen, Inc. Methods and dna constructs for high yield production of polypeptides
DE10227238A1 (de) 2002-06-19 2004-01-15 Wella Ag Hochaffine kosmetische Mittel
GB0216286D0 (en) * 2002-07-15 2002-08-21 Univ Leeds Network
EP2581383A1 (en) 2002-11-25 2013-04-17 Genencor International, Inc. Skin or hair binding peptides
AU2003299869A1 (en) 2002-12-26 2004-07-29 Gendron, Inc. Bariatric patient management system
US7220405B2 (en) 2003-09-08 2007-05-22 E. I. Du Pont De Nemours And Company Peptide-based conditioners and colorants for hair, skin, and nails
US20050054752A1 (en) 2003-09-08 2005-03-10 O'brien John P. Peptide-based diblock and triblock dispersants and diblock polymers
US7309482B2 (en) 2003-09-08 2007-12-18 E.I. Du Pont De Nemours And Company Long lasting waterproof sunscreen comprising metal oxide and peptide conditioner
US7285264B2 (en) 2003-09-08 2007-10-23 E.I. Du Pont De Nemours And Company Peptide-based body surface coloring reagents
US20050226839A1 (en) 2003-09-08 2005-10-13 Xueying Huang Pepetide-based body surface reagents for personal care
US7736633B2 (en) 2005-09-28 2010-06-15 E.I. Du Pont De Nemours And Company Method for enhancing effects of colorants and conditioners
US7928076B2 (en) 2005-12-15 2011-04-19 E. I. Du Pont De Nemours And Company Polypropylene binding peptides and methods of use
US7906617B2 (en) 2005-12-15 2011-03-15 E. I. Du Pont De Nemours And Company Polyethylene binding peptides and methods of use
US7632919B2 (en) 2005-12-15 2009-12-15 E.I. Du Pont De Nemours And Company Polystyrene binding peptides and methods of use
US7709601B2 (en) 2005-12-15 2010-05-04 E. I. Du Pont De Nemours And Company Nylon binding peptides and methods of use
US7700716B2 (en) 2005-12-15 2010-04-20 E. I. Du Pont De Nemours And Company Polytetrafluoroethylene binding peptides and methods of use
US7749957B2 (en) 2006-04-06 2010-07-06 E.I. Du Pont De Nemours And Company Clay-binding peptides and methods of use
US7732569B2 (en) 2006-10-19 2010-06-08 E.I. Du Pont De Nemours And Company Zein-based peptide tags for the expression and purification of bioactive peptides
US7662913B2 (en) 2006-10-19 2010-02-16 E. I. Du Pont De Nemours And Company Cystatin-based peptide tags for the expression and purification of bioactive peptides

Also Published As

Publication number Publication date
CN101835898A (zh) 2010-09-15
CA2689099A1 (en) 2009-01-29
JP5453261B2 (ja) 2014-03-26
JP2010534477A (ja) 2010-11-11
EP2173877A1 (en) 2010-04-14
EP2173877B1 (en) 2013-10-02
WO2009015165A1 (en) 2009-01-29
AU2008279186A1 (en) 2009-01-29
AU2008279186B2 (en) 2013-09-05
ES2439702T3 (es) 2014-01-24
US7678883B2 (en) 2010-03-16
US20090029412A1 (en) 2009-01-29
IL203002A0 (en) 2011-08-01
US7794979B2 (en) 2010-09-14
CN101835898B (zh) 2013-01-02
US20100136621A1 (en) 2010-06-03

Similar Documents

Publication Publication Date Title
CN101835898B (zh) 用于生物活性肽表达和纯化的可溶性标记
AU2020289750B2 (en) Engineered meganucleases with recognition sequences found in the human T cell receptor alpha constant region gene
AU2016333898B2 (en) Genetically-modified cells comprising a modified human T cell receptor alpha constant region gene
AU2019375975B2 (en) Artificial expression constructs for selectively modulating gene expression in excitatory cortical neurons
KR102528337B1 (ko) 정의된 서열 및 길이의 dna 단일 가닥 분자의 확장 가능한 생명공학적 생산
CN109661403A (zh) 前导序列修饰的葡糖淀粉酶多肽和具有增强的生物产物产生的工程化的酵母菌株
KR102823309B1 (ko) 재조합 콜라겐의 수산화를 제어하기 위한 효모 균주 및 방법
AU2016358063B2 (en) Functional expression of monooxygenases and methods of use
KR102191739B1 (ko) 변형된 구제역 바이러스 3c 프로테아제, 조성물 및 이의 방법
CN101939434B (zh) 用于在大豆中提高种子贮藏油脂的生成和改变脂肪酸谱的来自解脂耶氏酵母的dgat基因
TW202536186A (zh) 治療杜興氏(duchenne)肌肉失養症之大型突變熱點之aav載體介導之刪除
KR102804337B1 (ko) 콜라겐을 생산하기 위한 효모 균주 및 방법
KR20210149060A (ko) Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합
DK2768848T3 (en) METHODS AND PROCEDURES FOR EXPRESSION AND SECRETARY OF PEPTIDES AND PROTEINS
KR20110122672A (ko) 이소프렌 및 공-산물을 제조하는 방법
PT1984512T (pt) Sistema de expressão génica utilizando excisão-união em insetos
CN111757890A (zh) 发酵工艺
AU2018368610B2 (en) Production of a flavour compound in a host cell
KR20180081817A (ko) 감소된 clr1 활성을 갖는 사상 진균에서 단백질을 생산하는 방법
KR20230156365A (ko) 메타게놈 유래의 새로운 CRISPR-Cas 뉴클레아제
AU2016289877B2 (en) Method for gene optimization
CN114525304B (zh) 一种基因编辑的方法
US20030059870A1 (en) Recombinant bacterial strains for the production of natural nucleosides and modified analogues thereof
CN112280797A (zh) 一种可提高番茄中辅酶q10含量的联合载体及其构建方法和应用
KR100632764B1 (ko) 신규 프로모터 및 이 프로모터를 사용한 유전자 발현방법

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E601 Decision to refuse application
PE0601 Decision on rejection of patent

St.27 status event code: N-2-6-B10-B15-exm-PE0601

R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301