CN101829260B - Chinese medicine sachet for preventing and treating influenza and preparation method thereof - Google Patents
Chinese medicine sachet for preventing and treating influenza and preparation method thereof Download PDFInfo
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- CN101829260B CN101829260B CN2009100317010A CN200910031701A CN101829260B CN 101829260 B CN101829260 B CN 101829260B CN 2009100317010 A CN2009100317010 A CN 2009100317010A CN 200910031701 A CN200910031701 A CN 200910031701A CN 101829260 B CN101829260 B CN 101829260B
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Abstract
The invention discloses a Chinese medicine sachet for preventing and treating influenza and a preparation method thereof. A medicine product integrating health care with decoration is provided. The preparation of the sachet comprises the three steps of crude drug slice mixing, pulverizing and bagging. The sachet comprise total 15 pure Chinese medicinal plants, such as musk, borneol, Chinese atractylodes, agilawood, grass-leaved sweetflag, cassia bark, Chinese mugwort leaf, angelica root, hemlock parsley, ligusticum and the like. The sachet can act on the human body not only by the contact with skin and pores but also by sniffing and inhalation, has the efficacy for avoiding dirty, detoxifying, eliminating dampness with aromatics, clearing heat and promoting diuresis, causing resuscitation and restoring consciousness, and inhibits and kills various viruses and moulds in different degrees so as to achieve the effect of disease prevention. The sachet can be used as a personal pendant, a room decoration or automobile accessory to prevent various respiratory infectious diseases, such as code, influenza, epidemic cerebrospinal meningitis and the like.
Description
Technical field
The present invention relates to tcm field, relate in particular to and use Chinese herbal medicine to prevent and treat grippal Chinese medicine sachet and preparation method thereof as feedstock production a kind of.
Background technology
Sachet is that aromatotherapy is a kind of.Put down in writing according to the Classic of Mountains and Rivers: " preventable disease of Herba Lysimachiae foenum-graeci pendant "; The magnificent top in three state's epoch once with Flos Caryophylli, arrogate to oneself perfume (or spice) etc. and process sachet and wear with oneself, in case infections such as consumptive diseases; Qing Dynasty's " herbal book series " record: " Rhizoma Cyperi, gas fragrance is hot, can control cholera and vomit and diarrhoea ", or the like.Scientists thinks that the special aroma of some flowers and plants can rouse oneself people's spirit, to people's psychology, nervous system, respiratory system, blood circulation etc. all play a part to promote healthy, prevent and treat illness.There is research to confirm to contain the evaporable aromatic oil of ability in the plant, after respiratory mucosa absorbs, can promotes immune protein to produce, improve resistance, have the effect of inhibition, kill bacteria and protozoon.
The present invention's side is from the famous prescription " the pestilence pellet is warded off by Lingbao City " (seeing that " abide by and give birth to eight writing paper " volume four draws " Taoist Scriptures ") of Taoist school prevention pestilence, imperial family of the Qing Dynasty prophylactic imperial palace name side " safety made by the emperor order is red " (seeing " kind auspiciousness Guangxi hospital choosing view ") and code name side, Shanxi (seeing that Tang's " medical secrets of official " volume four draws handbook of Prescription for Emergency) the three first historical name sides of " warding off the pestilence powder "; Integrate, screen, refine through Nanjing University of Traditional Chinese Medicine's Traditional Chinese Medicine experts; Choose tripartite elite; Selected rare Chinese medicine; In addition experimental verification, initiative at last forms.
" the pestilence pellet is warded off by Lingbao City " is that Taoist school is used for the prescription commonly used of " warding off pestilence ", and Taoist priests process the ball shape with rare Chinese medicine, the occasion of concentrating crowds such as daily homework, religion appearance allusion quotations; All on fire, burn a ball; With disinfecting air, killing pathogenic bacteria, the propagation of the disease that keeps off infection." safety made by the emperor order is red " then is that the Qing Dynasty saves in separating the dirty pestilence of warding off, the heat clearing away of driving away summer heat, the prescriptions of Chinese medicine of invigorating the spleen and regulating the stomach royal spring and summer.The characteristics of this prescription are both oral administrations, and the sachet of also packing into is worn, and can also burn perfume, and effect is all good.The pestilence powder " is warded off " by the name side of Jin Daige flood, then is the achievement of medical practice before bimillennium, and war at that time is frequent, and pestilence is popular.According to the ancient book record, this first prescription effect is magical, and (pestilence is wreaked havoc, and the casualties is countless, even exterminates a family of the whole family " the pestilence phase inversion is dying, to exterminating a family; prolong and the stranger, no observer "; Too many in the dust people, the people who picks up a dead body has not had), miserable pestilence like this; As long as used on one's body " warding off the pestilence powder " be sprinkled upon, perhaps spread upon on the skin, just can exempt from infection!
The present invention picks the elite of above three first prescriptions exactly, the big doctor's of exhibition wisdom.
Summary of the invention
1, goal of the invention
The object of the present invention is to provide a kind of good looking appearance and can play the Chinese medicine sachet of the effect of warding off dirty detoxifcation, eliminating turbid pathogen with aromatics, clearing away heat-damp and promoting diuresis, the refreshment of having one's ideas straightened out, be used for diseases prevention, cure the disease, have one's ideas straightened out, keep healthy, decorate, and the method for preparing of this sachet is provided.
2, technical scheme
The objective of the invention is to realize through following technical scheme:
The Chinese medicine sachet is formed by artwork ornaments and pure Chinese medicine pocket, and artwork ornaments are used for decorating; Pure Chinese medicine pocket be used to encapsulate have ward off dirty detoxifcation, eliminating turbid pathogen with aromatics, clearing away heat-damp and promoting diuresis, the Chinese medicine of the refreshment effect of having one's ideas straightened out, the performance diseases prevention, cure the disease, have one's ideas straightened out, function of health care.Raw material is by Moschus 15-25g, Borneolum Syntheticum 40-60g, Rhizoma Atractylodis 200-240g, Lignum Aquilariae Resinatum 40-60g, Rhizoma Acori Graminei 80-120g, Cortex cinnamomi japonici (Ramulus Cinnamomi) 40-60g in the pure Chinese medicine pocket, and Folium Artemisiae Argyi 40-60g, Radix Angelicae Dahuricae 30-50g, Rhizoma Chuanxiong 30-50g, Rhizoma Ligustici 30-50g, Semen Arecae 30-50g, mentha leave 80-120g, Flos Caryophylli 40-60g, Herba Asari 40-60g, Rhizoma Paridis 80-120g form.The compound method of pure Chinese medicine pocket is divided into that decoction pieces mixes, pulverizes, the pack three process, will above ten Chinese medicine of the five flavours materials mixes and pulverizes, cross No. six sieves (100 order) sieve the fine powder medicine, according to each sachet 3-5g standard, the spice branch is packed in the bag, get pure Chinese medicine pocket.
This sachet can not only be through the skin pore contact; Can also act on human body through smelling, inhaling; Have the effect of warding off dirty detoxifcation, eliminating turbid pathogen with aromatics, clearing away heat-damp and promoting diuresis, the refreshment of having one's ideas straightened out, suppress to some extent and kill various bacteria and virus, mycete, thereby play the effect of diseases prevention.Can be used for carry-on suspension member, room decorating, automobile accessories with various respiratory road transmissions such as preventing cold, influenza, epidemic encephalitis disease.
We mainly are made up of aromatics Chinese medicine, and Chinese medicine thinks, the armaticity medicine is with removing dampness and to have one's ideas straightened out be two big main effects.Herbal pharmacology research proof; Armaticity Chinese medicine is the medicine of the special fragrance of some tools; Have and inspire healthy energy, remove heresy and ward off dirtyly, it is evil that expelling pathogenic factors from muscles is delivered, dispelling wind looses, and fragrant spleen invigorating, removing dampness are amusing; Be open to the custom have one's ideas straightened out, effect such as alleviating pain and detumescence, all special pharmacological character of these characteristics and armaticity Chinese medicine is relevant.Modern pharmacology confirms the multicomponent amalgam that the armaticity medicine normally is made up of various materials such as terpene hydrocarbons, arene, alcohols, ketone, aldehydes, ethers, esters, and wherein terpene hydrocarbons is the most important.Thereby can be fragrant quality assurance of drug the medicine that contains volatile oil component, to people's psychology, nervous system, respiratory system, blood circulation etc. all play a part to promote healthy, prevent and treat illness.
In sachet of the present invention, main component Moschus, Borneolum Syntheticum, Rhizoma Atractylodis, Lignum Aquilariae Resinatum, Rhizoma Acori Graminei, Cortex cinnamomi japonici (Ramulus Cinnamomi) all have powerful antivirus action.
Moschus is the dry secretions in the ripe male body note capsule of animal in deer family woods Moschus moschiferous Moschus berezovskii Flerov, horse Moschus moschiferous Mosifanicussifanicus Przewalski and former Moschus moschiferous Moschus moschiferus Linnaeus.Moschus, acrid in the mouth, warm in nature, QI exhaustion is fragrant, goes into the heart, spleen channel, has the merit of the refreshment of having one's ideas straightened out, promoting blood circulation to restore menstrual flow, reducing swelling and alleviating pain, is used to treat the card of sore swollen toxin, laryngopharynx swelling and pain."Shen Nong's Herbal Classic" will musk as a top grade, "the main provision of foul smells, temperature and malaria, epilepsy Chi, go three worms."" so the fragrance long drive of Moschus moschiferous is the Moschus moschiferous of meaning for the Li Shizhen (1518-1593 A.D.) meaning." the main fragrance ingredient of Moschus is muscone Moscone.Modern pharmacological research confirms that muscone can suppress escherichia coli and staphylococcus aureus growth external, and has tangible antiinflammatory action.
Borneolum Syntheticum is the processed goods (claim " borneol " or " borneol ") of Dipterocarpaceae aiphyllium plant Borneolum Syntheticum Dryobalanops aromatica Gaertn.f. resin or crystallization (claiming " Blumeae preparatum Tabellae ") that feverfew Herba Blumeae Balsamiferae Blumea balsamiferaDC. leaf extracts, or is that raw material is through the synthetic highly finished product of chemical method (claiming " BORNEOLUM SYNTHETICUM ") with Camphora, Lignum Pini Nodi wet goods.Borneol is to be close to purified dextro Borneolum Syntheticum (d-borneol), and Blumeae preparatum Tabellae is mainly left-handed Borneolum Syntheticum (L-borneol), and synthetic borneol is except that containing Borneolum Syntheticum, also contains a large amount of isoborneols (isoborneol).Borneolum Syntheticum, acrid in the mouth, hardship, cool in nature, go into the heart, lung meridian, the function refreshment of having one's ideas straightened out, clearing away heat to alleviate pain, treatment conjunctival congestion and swelling pain, sore throat, aphtha and pathopyretic ulcer card such as swell and ache.The merit of Compendium of Material Medica record Borneolum Syntheticum tool " logical all keys, the stagnated fire of loosing ", amplification on Canon of Materia Medica are summed up its use characteristics and are " a little less than the gesture of walking alone then, assistant makes then meritorious "." the herbal property of medicine is complete works of " said Borneolum Syntheticum can " control the swollen plug of sore throat.Control the vexed manic wild talk of children's's variola."" Compendium of Materia Medica "can be called" treatment pharyngitis, brain pain, nasal Xi, tooth pain, typhoid tongue out, pediatric depression pox, through various orifices, San Yu fire." Borneolum Syntheticum application clinically at present is very extensive.The in-vitro antibacterial test shows; Borneolum Syntheticum, isoborneol and synthetic borneol all have antibacterial action to staphylococcus aureus, beta hemolytic streptococcus, Streptococcus viridans, streptococcus pneumoniae and escherichia coli etc.; And bacteriostasis is arranged when low concentration; Bactericidal action is arranged during high concentration, and time of contact is long more, and antibacterial effect also strengthens thereupon.Borneolum Syntheticum also has an effect to the hog cholera vibrio.Its antibacterial mechanisms possibly be possibly see through cell wall to act on the somatic cells film, causes cell membrane damage, destroys the permeability that changes film, and medicine is got in the cell, acts on mitochondrial membrane and kills and wounds antibacterial.External direct antivirus test and chick embryo method show that Borneolum Syntheticum has the effect of the influenza virus of inhibition, and isoborneol can effectively suppress the I herpes simplex virus type, and its mechanism of action is the glycosylation that suppresses virus polypeptide.
Rhizoma Atractylodis are the dry rhizome of feverfew Atractylodes lancea (Thunb.) DC. Atractylodes lancea (Thunb.) DC. or Atractylis chinensis Atraetylodes Chinensis (DC.) Koidz..Rhizoma Atractylodis, acrid in the mouth, hardship, warm in nature, fragrance, bitter dry strong especially, specially go into taste, tool is drying damp and strengthening spleen, expelling wind and cold, the merit that makes eye bright, burnt turbid damp internal resistance in available the controlling, affection of exogenous wind-cold exterior syndrome.Existing pharmacy monograph Shennong's Herbal is the earliest just classified Rhizoma Atractylodis as top grade, can " keep away all bad odor " as far back as the Han dynasty with regard to Rhizoma Atractylodis on the books.Collective Notes to the Canon of Materia medica is also said Rhizoma Atractylodis and can " removed bad odor, remove the calamity rash ".Compendium of Material Medica is also put down in writing Rhizoma Atractylodis " can remove bad odor, sick at all times epidemic disease and dawn in year, other often burns Rhizoma Atractylodis to exorcise evil spirits gas, uses it so the disease of epidemic disease ".Say rhizoma atractylodis " dry native Li Shui lets out the drink dissolving phlegm, changes addiction and removes disease, wards off mountains and rivers miasma scrofula " in " beautiful Chinese catalpa medicine is separated ".Modern age Zhang Shanlei " book on Chinese herbal medicine justice " record " the Rhizoma Atractylodis abnormal smells from the patient is rich, and is more violent than the Rhizoma Atractylodis Macrocephalae, can be thorough thorough down, dampness and Xuanhua phlegm retention, repelling foulness with aromatics wins abnormal climates in four seasons, so the disease of epidemic disease is used it more.Can drive away foul bad odor, the territory of haze, spacious room should burn this thing and then occupy the people for a long time ".The medicine opinion of " China book on Chinese herbal medicine " also cloud " merit of Rhizoma Atractylodis focuses on and wards off dirty, dampness." Rhizoma Atractylodis mainly contain volatile oil, main component has atractylol, β-eucalyptol, atractylone and beta-elemene etc.Modern; The Rhizoma Atractylodis fumigation is used for air sterillizations such as public ward, delivery room, operating room, isolation ward more; Sterilizing power is strong, effect is held time length, and pathogenic microorganisms such as airborne antibacterial (staphylococcus aureus, escherichia coli, bacillus pyocyaneus and tubercule bacillus etc.), virus (influenza virus, rhinovirus, mumps virus), mycoplasma, fungus are all had the obvious suppression effect.Extracorporeal bacteria inhibitor test research confirms, Rhizoma Atractylodis extract all has bacteriostatic activity to multiple gram positive bacteria such as staphylococcus aureus, escherichia coli and gram-negative bacteria, and 15 kinds of funguses such as trichophyton are all had inhibitory action in various degree.The foreign study person finds that it has slight inhibitory action to HIV recombiant protein enzyme in the research that Rhizoma Atractylodis is suppressed the HIV active function.The mechanism of the inhibition microorganism of Rhizoma Atractylodis possibly be positions such as its volatile oil component amino and sulfydryl base of acting on microorganism, impels its nucleic acid and albuminous degeneration, makes microorganism metabolism generation obstacle and death.
Lignum Aquilariae Resinatum is the resiniferous heartwood of thymelaeceae plant Lignum Aquilariae Resinatum Aquilaria sinensis (Lour.) Gilg.Lignum Aquilariae Resinatum, hot, bitter, warm in nature, return spleen, stomach, kidney channel, the effect of tool promoting the circulation of QI to relieve pain, warming middle-JIAO to send down the adverse QI, helping inspiration to relieve asthma is used to treat the card of QI stagnated by cold chest and abdomen swelling and pain, gastrofrigid vomiting and dyspnea due to deficiency." record of this branches of the regular meridians " said Lignum Aquilariae Resinatum and can " treated the edema due to wind pathogen poison and swell, remove bad odor." Collective Notes to the Canon of Materia medica claims it and can " treat the grandular plague toxic swelling." " Haiyao Bencao, Oversea Materia Medica " year " main cardiac and abdominal pain, cholera, attacked by pestiferous factors heresy, clear people god of tuberculosis." " therapeutic method to keep the adverse QI flowing downwards is warded off evil to " property of medicine is examined " cloud, sensible refreshment." Lignum Aquilariae Resinatum mainly contains volatile ingredients such as baimuxinic acid, baimuxinal.Modern pharmacological research confirms that the Lignum Aquilariae Resinatum decoct all has bacteriostasis in various degree to human body tubercule bacillus, Bacillus typhi, shigella flexneri.
Rhizoma Acori Graminei is the dry rhizome of acorus gramineus araceae plant Acorus tatarinowii Schott..Rhizoma Acori Graminei, acrid in the mouth, hardship, slightly warm in nature is gone into the heart, liver, spleen channel, has waking up the patient from unconsciousness by dissipating phlegm, removing dampness circulation of qi promoting, the sharp numbness of dispeling the wind, the effect of reducing swelling and alleviating pain, the mind that the turbid heresy of phlegm-damp or expectorant hoodwinks due to thinking or the clear key is dazed and confused with controlling.Its main component is a volatile oil, comprise α-, β-and γ-asaricin.The successive dynasties book on Chinese herbal medicine is classified it as top grade, says Rhizoma Acori Graminei ability " happy hole, tonifying five ZANG-organs, logical nine orifices " like Shennong's Herbal, and " Taoist Scriptures's warp " said " Rhizoma Acori Graminei person, the elite of pasture and water, angle's miraculous cure "." abide by and give birth to eight writing paper " cloud its " can open wisdom, fill out gods, warm first down, tonify deficiency reduces just ".Modern pharmacological research confirms that the Rhizoma Acori Graminei water decoction all has inhibitory action in various degree to staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, escherichia coli, aerobacteria, Bacillus proteus, tubercule bacillus etc.α-asaricin all has inhibitory action to staphylococcus aureus, streptococcus pneumoniae and escherichia coli.Rhizoma Acori Graminei high concentration leachate has inhibitory action to common pathogenic dermatophytes.Rhizoma Acori Graminei water or alcohol extract have the diastole bronchus in addition, the smooth muscle spasm of relaxing, the pulmonary ventilation volume of improving, increase tidal volume and eliminate the phlegm, antiasthmatic effect.
Cortex cinnamomi japonici (Ramulus Cinnamomi) is claimed Cortex Cinnamomi again, is the dry bark of canella Cortex Cinnamomi Cinnamomum cassia Presl..Cortex Cinnamomi, property acrid-sweet flavor heat is returned spleen, kidney, the heart, Liver Channel, has that the fire of benefit is supporing yang, a dispersing cold for relieving pain, warming the meridian and promoting blood circulation, is used for treating the interior cold syndrome of kidney yang deficiency and deficiency of spleen-YANG and kidneyYANG more.Shennong's Herbal row Cortex cinnamomi japonici (Ramulus Cinnamomi) is top grade, and " acrid in the mouth temperature, main all kinds of diseases and ailments are supported spirit, and color, sharp joint, invigorating the spleen and replenishing QI.Engage logical making earlier for all medicines, clothes are logical refreshing for a long time, and it is not old to make light of one's life by commiting suicide.The brilliance of looking unfamiliar, eyebrow are well normal like the boy." " record of this branches of the regular meridians " say " hard muscles and bones, promoting blood circulation should be led hundred medicines, of a specified duration clothes, the angle, not old." the Compendium of Material Medica cloud " control the out-of-the-way aphonia sore throat of wind, lose blood by yang deficiency; Expelling the toxic substance from the body with tonic carbuncle variola can be drawn blood antiperspirant and suppurated." " property of medicine opinion " say " kill vegetation poison ", " cure mainly nine types of precordial pain, kill three worms ".Cortex Cinnamomi mainly contains volatile oil, mainly contains cinnamic aldehyde, cinnamic acid etc.Experimentation shows that Cortex Cinnamomi all has certain inhibitory action to acute and chronic inflammatory reaction., capillary permeability increase swollen for rat toes due to the carrageenin all has inhibitory action.The Cortex Cinnamomi decoct is external all to have tangible bacteriostasis to escherichia coli, dysentery bacterium, Bacillus typhi, staphylococcus aureus, Candida albicans, and dermatophytes is had very strong inhibitory action.Cortex Cinnamomi extract is inhibited to the first type capital 68-1 of section strain of influenza virus Asia and Orphan virus (ECH011).
3, beneficial effect
An important feature of sachet of the present invention be have diseases prevention, cure the disease, have one's ideas straightened out, keep healthy, the function of decoration.The present invention shows that through clinical research following advantage is arranged:
1. has the effect of warding off dirty detoxifcation, eliminating turbid pathogen with aromatics, clearing away heat-damp and promoting diuresis, the refreshment of having one's ideas straightened out.
2. experimentation confirms that antiviral effect is good.Experimentation confirms that this sachet can alleviate that pneumonopathy becomes due to first type, the Influenza B virus; Reduce the lung exponential quantity; Have the first of alleviating type, Influenza B virus infection pneumonopathy change effect, and can obviously reduce the death toll of first type, Influenza B virus infecting mouse, prolong mice survival natural law.Point out this sachet to have the effect of anti-first type, Influenza B virus.
3. there is not obvious toxicity.
The specific embodiment
Embodiment 1, a kind of Chinese medicine composition and weight proportion that prevents and treat grippal sachet
The Chinese medicine of sachet is formed and weight proportion is: Moschus 15-25g, Borneolum Syntheticum 40-60g, Rhizoma Atractylodis 200-240g, Lignum Aquilariae Resinatum 40-60g, Rhizoma Acori Graminei 80-120g, Cortex cinnamomi japonici (Ramulus Cinnamomi) 40-60g, Folium Artemisiae Argyi 40-60g, Radix Angelicae Dahuricae 30-50g, Rhizoma Chuanxiong 30-50g, Rhizoma Ligustici 30-50g, Semen Arecae 30-50g, mentha leave 80-120g, Flos Caryophylli 40-60g, Herba Asari 40-60g, Rhizoma Paridis 80-120g.
Embodiment 2, a kind of method for preparing of preventing and treating grippal sachet, and its preparation process is:
1, producing artictic decoration articles
Get the artwork ornaments that exquisite cloth, silk textile make 12 symbolic animals associated with a 12 year cycle form cryptomere, all kinds of artwork ornaments all leave the position and the space of the pure Chinese medicine pocket of assembling.
2, pure Chinese medicine pocket preparation
1. make pocket: the pocket that makes different volumes by the series of products requirement with cloth.
2. Chinese medicine is got the raw materials ready: by material rate take by weighing Moschus, Borneolum Syntheticum, Rhizoma Atractylodis, Lignum Aquilariae Resinatum, Rhizoma Acori Graminei, Cortex cinnamomi japonici (Ramulus Cinnamomi), Folium Artemisiae Argyi, the Radix Angelicae Dahuricae, Rhizoma Chuanxiong, Rhizoma Ligustici, Semen Arecae, mentha leave, Flos Caryophylli, Herba Asari, that Rhizoma Paridis ten Chinese medicine of the five flavours materials are drunk raw sheet is subsequent use.
3. prepare spice: earlier ten Chinese medicine of the five flavours materials are drunk raw sheet and pulverize, crossing No. six sieves (100 order), to sieve into mixing fine powders subsequent use.
4. spice pack: each sachet 3-5g, pack spice in the pocket into, pure Chinese medicine pocket.
3, sachet pack and usage
Pure Chinese medicine pocket pack in the Chinese zodiac ornaments the Chinese medicine sachet, use plastic film sealing, with before tearing pocket sealing plastic film outward, the pure Chinese medicine sachet fragrant abnormal smells from the patient that both gently shed plays the effect of dirty detoxifcation, eliminating turbid pathogen with aromatics, clearing away heat-damp and promoting diuresis, the refreshment of having one's ideas straightened out.
Embodiment 3, the zoopery of antivirus action
1 experiment material
1.1 medicine and reagent
Ward off dirty sachet, be broken into the impalpable powder sachet of packing into, each sachet drug powder 2 gram by Moschus, Borneolum Syntheticum, Rhizoma Atractylodis, Lignum Aquilariae Resinatum, Rhizoma Acori Graminei, Cortex cinnamomi japonici (Ramulus Cinnamomi) etc. 15 flavor Chinese medicine powders; Nutrient broth; Chicken red blood cell, fresh Sanguis Gallus domesticus is with subsequent use after the normal saline washing 3 times; Embryo Gallus domesticus, the 9-10 age in days.
1.2 experiment is with virus
Influenza A virus A/PR/8/34, the anti-98-76 in Influenza B virus B/ capital, Virology Inst., China Academy of Preventive Medicine Sciences provides.
1.3 laboratory animal
The ICR mice is provided by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center.Laboratory animal occupancy permit: SCXK (Soviet Union) 2002-0053.
1.4 experimental apparatus
Clean work station; Incubator; 24 hole microwell plates; Self-control 20 * 20 * 20cm lucite transparent vessel.
1.5 culture medium and medicine preparation
Nutrient broth: precision takes by weighing nutrient broth medium 18g, and adding distil water is to 1000ml, and after heating made dissolving, packing 10ml, 4.5ml, 1.6ml, 1.0ml were subsequent use behind 116 ℃ of 20min autoclavings.
1.6 experiment condition
Mice feeds full-valence pellet feed before and after the administration, freely drinks water 22 ± 2 ℃ of room temperatures, temperature 55-65 ℃.2 ward off the effect of dirty sachet to influenza A virus A/PR/8/34
2.1 the influenza A virus Embryo Gallus domesticus increases the poison test
Get instar chicken embryo on the 9th,, place aseptic indoor 25% ethanol and 2% iodine tincture and sterilize respectively after 2 times in egg candler marked air chamber; Grind an osculum (needle point size) gently with emery wheel; Get influenza A virus strain powder and add 0.5ml normal saline mixing (dividing 2-3 time), be diluted to 2ml with normal saline after the sucking-off, every the egg inoculation 0.2ml influenza A virus venom in piping and druming back; Behind paraffin sealing, put 37 ± 0.5 ℃ of incubators and cultivate 48h.The taking-up Embryo Gallus domesticus is put in 4 ℃ of refrigerators and is spent the night, and take out next day, after 75% ethanol and the sterilization of 2% iodine tincture, removes the paraffin sealing place; Open air chamber, draw allantoic fluid, discard muddy allantoic fluid with aseptic straw; More than be collected as and increase poison once, be used for experiment, experiment previous crops hemagglutination test after repeating 3 times.
2.2 hemagglutination test
Get 24 hole microwell plates, every hole adds normal saline 0.5ml, (the 1st pipe adds normal saline 0.9ml); To increase and add the 2nd hole 0.5ml after 3 viral allantoic fluid 0.1ml of poison add the 1st hole mixing; Be diluted to the 8th hole successively, the 9th hole does not add virus, does blank; Every subsequently hole adds 0.25ml 0.5% chicken red blood cell, gently observed and recorded behind the rearmounted room temperature placement of the mixing 2h.
Observation caliber:
++ ++ one deck erythrocyte is hates the medicated cap shape evenly to be laid at the bottom of the hole
+++basically is the same, but the edge is thinner
++ blood cell forms a ring-type in the pipe end, but little coagulation piece is arranged all around
+ blood cell forms little group in the pipe end, and the edge is smooth, and is neat
Experimental result is judged: the blood clotting titre is having more than 640 2,3,8,9,10,14, No. 16, can be used for infection experiment in the body.
2.3 influenza a virus infection mice lethal dose (LD50) is measured
Get 56 of ICR mices, body weight 13-16g, male and female half and half are divided 7 groups at random; Every group 8, get hemagglutination test 2 of the allantoic fluids more than 640 (different numbering), after the piping and druming; With 10 times of dilutions, under the ether light anaesthesia, every group drips 1 virus concentration; Observe death toll in the 14d, press Reed Muench method and calculate LD50, LD50 is 10 as a result
-2.11, promptly virus concentration is 10
-2.11The time, can make the infecting mouse half dead.
2.4 ward off of the influence of dirty sachet to influenza A virus A/PR8/34 Lung Index of mice infected by Influenza virus
Get 48 of ICR mices, body weight 13-16g, male and female half and half are divided into 4 groups by body weight at random, 12 every group.[1] normal control group; [2] model group; [3] ward off dirty sachet I group: ward off dirty sachet (4g) with 2 and be fixed in container head; [4] ward off dirty sachet II group: ward off dirty sachet (8g) with 4 and be fixed in container head.Below respectively organize mice and all place in the container, 4 mices of each container are after 5 days, under the ether light anaesthesia; Increasing poison 3 influenza A virus allantoic fluids (hemagglutination test is more than 640) collunarium infecting mouse, every Mus 30ul, (20 LD50 lethal doses), fasting can't help water 12 hours before the experiment; Experiment mice on the same day is weighed, and takes off cervical vertebra and puts to death, and dissects, and observes pulmonary lesion; Get lung and weigh, calculate the lung exponential quantity, lung index suppression ratio, relatively each group difference.The result sees table 1.
Table 1 is warded off the influence of dirty sachet to influenza A virus A/PR8/34 Lung Index of mice infected by Influenza virus
* compare with model group p＜0.05 * * p＜0.01
Experimental result shows: ward off dirty sachet I group and II group and can alleviate all that pneumonopathy becomes reduction lung exponential quantity due to the influenza A virus.Lung index suppression ratio is respectively: 29.4%, 28.9%, and prompting is warded off dirty sachet influenza a virus infection is had the pneumonopathy of alleviating change effect.
2.5 ward off dirty sachet to the dead protective effect of influenza A virus A/PR8/34 infecting mouse
Get 80 of ICR mices, body weight 13-16g, male and female half and half are divided into 4 groups by body weight at random, 20 every group.[1] normal control group; [2] model group; [3] ward off dirty sachet I group: ward off dirty sachet (4g) with 2 and be fixed in container head; [4] ward off dirty sachet II group: ward off dirty sachet (8g) with 4 and be fixed in container head.Below respectively organize mice and all place in the container, 4 mices of each container are after 5 days; In administration same day under the ether light anaesthesia, to increase the influenza A virus allantoic fluid of poison 3 times, (hemagglutination test is more than 640) collunarium infecting mouse; Every Mus 30ul, (20 LD50 lethal doses), the record collunarium infects animal dead situation in 14 days; Calculate survival natural law, survival rate, the comparable group differences.The result sees table 2.
Table 2 is warded off dirty sachet to the dead protective effect of influenza A virus A/PR8/34 infecting mouse
* compare with model group p＜0.05 * * p＜0.01
Experimental result shows: ward off dirty sachet II group and can obviously reduce the death toll of influenza a virus infection mice, prolong the survival natural law, relatively have significant difference (p＜0.05) with model group, the effect that dirty sachet has anti-influenza A virus is warded off in prompting.
3 ward off the effect of dirty sachet to the anti-98-76 in Influenza B virus B/ capital
3.1 the Influenza B virus Embryo Gallus domesticus increases the poison test
Get instar chicken embryo on the 9th; In egg candler marked air chamber, place aseptic indoor 25% ethanol and 2% iodine tincture and sterilize respectively and grind an osculum (for the needle point size) gently with emery wheel after 2 times, get Influenza B virus strain powder and add 0.5ml normal saline mixing (dividing 2-3 time); Be diluted to 2ml with normal saline after the sucking-off; Every the egg inoculation 0.2ml Influenza B virus venom in piping and druming back behind paraffin sealing, is put 37 ± 0.5 ℃ of incubators and is cultivated 48h.The taking-up Embryo Gallus domesticus is put in 4 ℃ of refrigerators and is spent the night, and take out next day, after 75% ethanol and the sterilization of 2% iodine tincture, removes the paraffin sealing place; Open air chamber, draw allantoic fluid, discard muddy allantoic fluid with aseptic straw; More than be collected as once antitoxinly, be used for experiment after repeating 2 times, experiment previous crops hemagglutination test.
3.2 hemagglutination test
Get 24 hole microwell plates, every hole adds normal saline 0.5ml, (the 1st pipe adds normal saline 0.9ml); To increase and add the 2nd hole 0.5ml after 3 viral allantoic fluid 0.1ml of poison add the 1st hole mixing; Be diluted to the 8th hole successively, the 9th hole does not add virus, does blank; Every subsequently hole adds 0.25ml 0.5% chicken red blood cell, gently observed and recorded behind the rearmounted room temperature placement of the mixing 2h.
Observation caliber:
++ ++ one deck erythrocyte is hates the medicated cap shape evenly to be laid at the bottom of the hole
+++basically is the same, but the edge is thinner
++ blood cell forms a ring-type in the pipe end, but little coagulation piece is arranged all around
+ blood cell forms little group in the pipe end, and the edge is smooth, and is neat
Experimental result is judged: the blood clotting titre is having chick embryo allantoic liquid 1,4,6,8,11,13,14,17, No. 18 more than 640, can be used for infection experiment in the body
3.3 Influenza B virus infecting mouse lethal dose (LD50) is measured
Get 56 of ICR mices, body weight 13-16g, male and female half and half; Divide 7 groups at random, 8 every group, get hemagglutination test 2 of the allantoic fluids more than 640 (different numbering); After the piping and druming, with 10 times of dilutions, under the ether light anaesthesia; Every group drips 1 virus concentration, observes death toll in the 14d, presses Reed Muench method and calculates LD50.LD50=10 as a result
-1.71, promptly virus concentration is 10
-1.71The time, can make the infecting mouse half dead.
3.4 ward off of the influence of dirty sachet to the anti-98-76 Lung Index of mice infected by Influenza virus in Influenza B virus B/ capital
Get 48 of ICR mices, body weight 13-16g, male and female half and half are divided into 4 groups by body weight at random, 12 every group.[1] normal control group; [2] model group; [3] ward off dirty sachet I group: ward off dirty sachet (4g) with 2 and be fixed in container head; [4] ward off dirty sachet II group: ward off dirty sachet (8g) with 4 and be fixed in container head.Below respectively organize mice and all place in the container, 4 mices of each container, after 5 days, in administration same day under the ether light anaesthesia; Increasing poison 3 Influenza B virus allantoic fluids (hemagglutination test is more than 640) collunarium infecting mouse, every Mus 30ul, (20 LD50 lethal doses), fasting can't help water 12 hours before the experiment; Experiment mice on the same day is weighed, and takes off cervical vertebra and puts to death, and dissects, and observes pulmonary lesion; Get lung and weigh, calculate the lung exponential quantity, lung index suppression ratio, relatively each group difference.The result sees table 3.
Table 3 is warded off the influence of dirty sachet to the anti-98-76 Lung Index of mice infected by Influenza virus in Influenza B virus B/ capital
* compare with model group p＜0.05 * * p＜0.01
Experimental result shows: ward off dirty sachet II group and can alleviate that pneumonopathy becomes due to the Influenza B virus, reduce the lung exponential quantity.Lung index suppression ratio is 28.7%, and dirty sachet is warded off in prompting, and infection has the pneumonopathy of alleviating change effect to Influenza B virus.
3.5 ward off dirty sachet to the dead protective effect of the anti-98-76 infecting mouse in Influenza B virus B/ capital
Get 80 of ICR mices, body weight 13-16g, male and female half and half are divided into 4 groups by body weight at random, 20 every group.[1] normal control group; [2] model group; [3] ward off dirty sachet I group: ward off dirty sachet (4g) with 2 and be fixed in container head; [4] ward off dirty sachet II group: ward off dirty sachet (8g) with 4 and be fixed in container head.Below respectively organize mice and all place in the container, 4 mices of each container are after 5 days; In administration same day under the ether light anaesthesia, to increase poison 3 Influenza B virus allantoic fluids (hemagglutination test is more than 640) collunarium infecting mouse, every Mus 30ul; (20 LD50 lethal doses); The record collunarium infects animal dead situation in 14 days, numeration survival natural law, survival rate, comparable group differences.The result sees table 4.
Table 4 is warded off dirty sachet to the dead protective effect of the anti-98-76 infecting mouse in Influenza B virus B/ capital
* compare with model group p＜0.05 * * p＜0.01
Experimental result shows: ward off dirty sachet I group and II group the Influenza B virus dead mouse is had the significant protection effect; And can obviously prolong Influenza B virus infecting mouse survival natural law; Relatively have notable difference (p＜0.05) with model group, the effect that dirty sachet has anti-Influenza B virus is warded off in prompting.
4 brief summaries
Ward off dirty sachet and can alleviate that pneumonopathy becomes due to first type, the Influenza B virus, reduce the lung exponential quantity, have the first of alleviating type, Influenza B virus infects pneumonopathy change effect.And ward off the death toll that dirty sachet can obviously reduce first type, Influenza B virus infecting mouse, prolong mice survival natural law.More than the effect that dirty sachet has anti-first type, Influenza B virus of warding off is all pointed out in experiment.
Claims (2)
1. one kind is prevented and treats grippal Chinese medicine sachet; Form by art and pure Chinese medicine pocket; It is characterized in that pure Chinese medicine pocket weight is 3-5g; It is processed by the following weight proportion raw material: Moschus 15-25g, Borneolum Syntheticum 40-60g, Rhizoma Atractylodis 200-240g, Lignum Aquilariae Resinatum 40-60g, Rhizoma Acori Graminei 80-120g, Cortex cinnamomi japonici (Ramulus Cinnamomi) 40-60g, Folium Artemisiae Argyi 40-60g, Radix Angelicae Dahuricae 30-50g, Rhizoma Chuanxiong 30-50g, Rhizoma Ligustici 30-50g, Semen Arecae 30-50g, mentha leave 80-120g, Flos Caryophylli 40-60g, Herba Asari 40-60g, Rhizoma Paridis 80-120g.
2. method for preparing the said Chinese medicine sachet of claim 1 is characterized in that: earlier that Moschus, Borneolum Syntheticum, Rhizoma Atractylodis, Lignum Aquilariae Resinatum, Rhizoma Acori Graminei, Cortex cinnamomi japonici (Ramulus Cinnamomi), Folium Artemisiae Argyi, the Radix Angelicae Dahuricae, Rhizoma Chuanxiong, Rhizoma Ligustici, Semen Arecae, mentha leave, Flos Caryophylli, Herba Asari, 15 kinds of Chinese medicines of Rhizoma Paridis is selected respectively, and make the medicine cleaning pure; Dry the post-treatment porphyrize, cross sieve (100 order) sieve aperture No. six, then by required weight proportion and after fully mixing; By the form of artwork ornaments and the requirement of volume; Thin medicated powder 3-5g packing is gone in the pocket, use plastic film sealing, get pure Chinese medicine bag; With in the packed artificial skill ornament of pure Chinese medicine, get pure Chinese medicine sachet at last.
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| CN108310309A (en) * | 2018-04-27 | 2018-07-24 | 江苏护理职业学院 | A kind of inducing resuscitation sachet and preparation method thereof |
| CN111265603A (en) * | 2020-03-18 | 2020-06-12 | 郭潇 | A Chinese medicinal powder for preventing and treating upper respiratory infection |
| CN112472744A (en) * | 2020-12-14 | 2021-03-12 | 山东汉方制药有限公司 | Epidemic prevention traditional Chinese medicine composition and sachet prepared from same |
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