CN101744924A - Multiple-effect Chinese herbal medicine compound of bacterial diseases of turbot - Google Patents
Multiple-effect Chinese herbal medicine compound of bacterial diseases of turbot Download PDFInfo
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Abstract
The invention relates to a multiple-effect Chinese herbal medicine compound for bacterial diseases of turbot (Scophthalmus maximus). The compound comprises the following pharmaceutical ingredients: cortex phellodendri chinensis, fructus forsythiae, flos caryophyllata, bupleurum chinense and liquorice; the pharmaceutical ingredients are mixed according to a weight ratio of 2:1:1:1:1, ground screened by a screen with 200 meshes and mixed uniformly. The medicine is a composite antibacterial and immunity-improving multiple-effect Chinese herbal medicine formula. It is proved by clinical tests that the medicine can be effectively applied to preventing and treating bacterial diseases such as turbot ascites, enteritis, reddish body, and the like. The medicine can treat the diseases in the ways of mixing with baits uniformly, taking orally or taking medicated bath, and the curative ratio can reach greater than 80%. The pharmaceutical ingredients of the formula belong to natural Chinese herbal medicines, meet the food safety requirements, have no toxic or side effects on the growth of turbot, and can reduce and replace the utilization of antibiotics. The medicine has simple application method, low dosage and low cost, is suitable for production of aquaculture production of turbot, and can improve the survival ratio and the quality of products.
Description
Technical field:
The invention belongs to the special-purpose Chinese herbal medicine of preventing and treating turbot important bacteria disease, is the Chinese herb compound that prevents and treat at turbot main bacteria disease in recent years under cultivating condition.
Background technology:
Turbot becomes the pillar industry of China's sea-farming rapidly with advantages such as its easy raising, fast growth, delicious meat, and annual directly economic benefit reaches more than 40 hundred million yuan, has obtained remarkable economical and social benefit.But unordered expansion and lack of standardizationization running along with aquaculture industry cause disease frequently to take place.After disease produces, blindly use antibiotic phenomenon ubiquity, so that it is residual medicine to occur, influences product quality safety.In November, 2006, " the residual incident of turbot medicine " causes the turbot industry to fall into low ebb.This incident makes vast breed dealer suffer greatly economic loss, also recognizes antibiotic negative effect simultaneously.
Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science takes the lead in that turbot has been carried out disease popularity disease comprehensive, system and learns research, the tangible disease symptoms of kind surplus discovery breed turbot has 10.Wherein bacterial disease sickness rate height such as ascites disease, enteritis disease, fin rot disease, red abdominal shell disease, spread speed are fast, pathogenic strong, have caused serious economy loss (table one) to the raiser.Bacterial disease is the principal disease that turbot is cultured, and antibiotic becomes raiser's main anti-disease medicines naturally, and its medicine is residual and quality problems aquatic products are also very severe.Therefore the alternative medicine of developing safety and low toxicity, green high-efficient is extremely urgent.
Before the present invention forms, do not see the play-by-play of relevant herbal control bacterial diseases of turbot.
Table one: culture turbot main bacteria disease and cause a disease former
| Time | The place | Disease | Pathogen | The author |
| 2004 | Laizhou City, Shandong, Qingdao City and Rongcheng City | Intestinal epithelial cell, fragment of tissue that a large amount of necrosis come off and a large amount of antibacterials that grow are arranged in the intestinal, and color is a yellow-white. | Vibrio alginolyticus (Vibrio alginolyticus) and turbot vibrio (Vibrio scophthalmi) | Wang Yingeng, just opening, Qin Lei |
| 2004.05-2005.09 | Weifang, Yantai, Weihai and Qingdao | The multiple focal necrosis of kidney is with exudative inflammation reaction and granulomatous generation; Outward appearance is expanded unusually, and normal structure changes white pus infections or bean curd scoriform into. | Tarda (Edwardsiellosi) | Wang Yingeng, Qin Lei, just open |
| 2005 | Rongcheng City, Jiaonan City and Qingdao City | Watch abdominal cavity tympanites, abdominal part protuberance outward, dissect the back and find that inside, thoracic cavity is full of colourless or weak yellow liquid. | Shark vibrio (Vibrio carchariae) | Wang Yingeng, just opening, Qin Lei |
| Time | The place | Disease | Pathogen | The author |
| Calendar year 2001 | Laizhou City, Shandong, Haiyang City, Qingdao City | Lateral fin, tail fin fester hemorrhage, and oral area even whole head are rubescent sometimes, and each internal organs all has pathological changes in various degree | Vibrio anguillarum (Vibrio anguillarum) | Just open, Wang Yingeng, Korean monarch |
Summary of the invention:
The purpose of this invention is to provide a kind of Chinese herb compound, can treat multiple bacterial diseases of turbot, and these bacterial diseasees are had preventive effect.By using this medicine can significantly improve the premunition and the survival rate of culturing turbot, ensure the stability of breeding production.
The present invention adopts following technical scheme:
Ingredient and proportioning are: ingredient is Cortex Phellodendri, Fructus Forsythiae, Flos Caryophylli, Radix Bupleuri, Radix Glycyrrhizae; The medical herbs granularity is 200 orders, and medicine according to weight proportion is: 2: 1: 1: 1: 1.
The present invention and prior art contrast are characterized in:
1, the present invention can accomplish " potion multiple-effect ", can treat ascites disease, enteritis disease, eperythrozoonosis, fin rot disease various bacterial disease simultaneously.
2, the present invention can use seedling stage and adult fish, and cure rate can reach more than 80%, and prevention and therapeutic effect are remarkable.
3, the pure Chinese herbal and crude drugs preparations of the present invention, green high-efficient, noresidue can reduce and substitute antibiotic use, have important function to improving aquatic product quality.
4, cost of the present invention is low, easy to use, respond well, is easy to promote the use of.
Description of drawings:
Fig. 1: the different additions of Chinese herbal medicine are to the variation diagram of nitricoxide synthase (NOS);
Fig. 2: the different additions of Chinese herbal medicine are to the influence of lysozyme:
Fig. 3: the Chinese herbal medicine of the different additions of throwing something and feeding is to the influence of acid phosphatase.
Specific implementation method:
Be to be described in detail prescription of the present invention and using method in conjunction with the accompanying drawings below by example:
The present invention makes by following technical scheme: illustrate epidemiologic feature, separation and purification cause of disease, artificial challenge test definite pathogenic former; Through pharmacodynamics test, preferred compositions prescription, the check of turbot immune indexes, processes such as clinical trial and cure rate check thereof.
The flow process that this prescription is realized is: screening formula and proportioning → experiment Chinese herbal medicine is to the influence → Chinese herbal medicine clinical trial → using method of the turbot nonspecific immunity factor.
1, prescription and proportioning screening:
This prescription mainly is the common pathogen at turbot, and as turbot vibrio, tarda, shark vibrio, Vibrio anguillarum and vibrio alginolyticus, screening has the Chinese herbal medicine formula of inhibition or killing effect, for production practices provide theoretical direction and reference.Its method is: Herba Houttuyniae, the Radix Astragali, Radix Glycyrrhizae, Fructus Forsythiae, Radix Gentianae, Flos Lonicerae, Radix Isatidis, Fructus Gardeniae, Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri, Radix Et Rhizoma Rhei, Folium Isatidis, Herba Andrographis, Galla Chinensis, Radix Bupleuri, Cortex Acanthopancis, Poria, Fructus Mume, the Radix Pulsatillae, Pericarpium Granati, Radix Achyranthis Bidentatae, Radix Codonopsis, Herba Violae, Cortex Fraxini, Olibanum, Myrrha, Flos Caryophylli, Caulis Sargentodoxae and 35 kinds of Chinese herbal medicine of the Cortex Eucommiae and above-mentioned isolating pathogenic bacterium are carried out drug sensitivity test, the folk prescription that the primary election fungistatic effect is good.Through different compatibilities,, finally obtain optimum formula by external fungistatic effect checking (table two) and clinical trial.
Filtering out by folk prescription has better fungistatic effect and meets 5 kinds of folk prescription Chinese herbal medicine of compatibility requirement the main pathogenic bacterium of turbot: be respectively Cortex Phellodendri, Fructus Forsythiae, Flos Caryophylli, Radix Bupleuri, Radix Glycyrrhizae.Carry out the Chinese herbal medicine proportioning according to orthogonal experiment and drug matching principle, obtaining external antibacterial best compatibility is 2: 1: 1: 1: 1, its compound recipe all had the obvious suppression effect to above-mentioned five kinds of main pathogenic bacterium of turbot.Medical herbs granularity 200 orders.
Table two compound Chinese herb is to the extracorporeal bacteria inhibitor test of 5 kinds of antibacterials
The herbal liquid medicine original concentration is 0.2g/ml, and in the table 1/20,1/40...... represents 4 times of Chinese herbal medicine original liquid dilutions respectively, 8 times, "+" represents the antibacterial that do not grow under this concentration, and "-" represents the antibacterial that grown under this concentration.
2, Chinese herbal medicine is to the influence of the turbot nonspecific immunity factor:
Experiment is divided into four groups, and first group of 2% compound Chinese herb of throwing something and feeding throws something and feeds 4%, the three group that to throw something and feed 8%, the four group be the matched group normal feedstuff of throwing something and feeding for second group.The experimental water salinity is 25 ‰, and 16 ℃ of temperature are thrown something and fed for twice in every morning and afternoon; Blood sampling in per seven days is once measured nitricoxide synthase, lysozyme, acid phosphatase in the serum.Compare with matched group, the Chinese herbal feed of the variable concentrations of throwing something and feeding all has significantly raising effect to above 3 kinds of enzyme indexs, and reaches maximum at the 28th day, specifically sees accompanying drawing 1,2,3.
In sum, add not commensurability Chinese herbal medicine to the effect that all is significantly improved of nitricoxide synthase, lysozyme, the acid phosphatase of turbot.After overall the comparison, determining to add 2% Chinese herbal medicine promptly has good result.
3, Chinese herbal medicine clinical trial:
Healthy turbot is in the sea water medicinal liquid of 200ppm (ppm is meant that the 1mg solute is dissolved in the 1L solvent), and through 96 hours dipping toxicity tests, physiological status was good, the poisoning sign do not occur.Use the clinical trial that this Chinese herb compound has carried out prevention and treated 5 the ill turbot in different breeding field in area, Shandong, respond well (table three).
The clinical trial of table three compound Chinese herb
| Time | The place | Symptom | Using method | Effect |
| 2007.10 | The Laizhou | Turbot about 50 grams has ascites through dissection, and in vain just, it is relatively poor to ingest, and occurs a small amount of dead. | Slow fire boiled 1 hour, 20ppm dipping 3 times, every |
Basic healing, fish body health |
| 2008.04 | Gaizhou City | That the turbot body has is rubescent, mashed fin, enteritis symptom, and it is relatively poor to ingest. | Dipping is the same, and raises and throw something and feed continuous 8 days with 3% companion. | Cure rate is more than 80%, and is respond well |
| 2009.08 | Qingdao | Turbot fry ascites disease, ingest relatively poor. | Dipping is the same, and 2% companion raises and throws something and feeds continuous 10 days. | Cure rate 90% |
| 2009.10 | The Laizhou | Turbot has ascites, enteritis symptom, and body constitution is weak, it is poor to ingest, the phenomena of mortality are arranged. | Dipping is the same, and raises and throw something and feed continuous 10 days with 2% companion. | Cure rate 85%, therapeutic effect is good |
| 2009.12 | Wendeng City | Prevention and treatment ascites disease of turbot scophthalmus maximus, enteritis disease | Prevention: 2% companion raises dispensing, continuous 7 days.Drug withdrawal is reused after 10 days again.Treatment: 20ppm dipping 3 times, 3% companion raises dispensing, continuous 10 days. | Preventive effect is remarkable, and compared with the control, disease incident reduces by 21%; Therapeutic effect is obvious, and survival rate reaches 98.7%. |
By the clinical prevention evidence: raise the prevention of throwing something and feeding with the 20g/kg companion, preventive effect is good, can effectively reduce the generation of disease; During treatment, raise by 20ppm dipping and 30g/kg companion and to throw something and feed, cure rate reaches more than 80%, and therapeutic effect is remarkable.This Chinese herb compound all has good prevention effect to ascites disease of turbot scophthalmus maximus, enteritis disease, eperythrozoonosis, fin rot disease.The present invention utilizes Chinese herb compound, accomplish " potion multiple-effect ", can treat ascites disease, enteritis disease, eperythrozoonosis, fin rot disease various bacterial disease simultaneously, to reducing and replacing antibiotic use, improve and culture survival rate and product quality, ensure that the breeding production stable and high yields has crucial meaning.
4, using method:
Prophylactic: Chinese herb compound is pressed the 20g/kg feedstuff mix, with throwing something and feeding every day 2 times, continuous 7 days behind the binding agent enwrapped granule feedstuff surface; The pharmaceutical chemistry of throwing something and feeding once more after 10 days at interval.
Medicine for treatment:, boiled 20ppm dipping 3 times, every day 1 time, each 8 hours 1 hour according to this compound Chinese herb and 1: 10 ratio slow fire of tap water according to dipping water body size.The companion raises dispensing, continuous 10 days according to this compound Chinese herb of 30g/kg.For guaranteeing drug effect, dipping time requirement is more than 8 hours.
Coastal in northern China, turbot cultures to develop rapidly becomes a pillar industry.But because the industry development time is short, practitioner's technical merit is uneven.Exist distinct issues to be that culture diseases takes place frequently, medication is lack of standardization at present even problem such as abuse forbidden drug, so that the residual phenomenon of marketable fish medicine occurs, harm consumer is healthy.The aquatic products drug residue exceeds standard becomes the bottleneck of restriction turbot industry development.In order fundamentally to address this problem, the Chinese herbal medicine of exploitation green high-efficient is an important channel.The invention belongs to compound Chinese herb, can accomplish " potion multiple-effect ", treat various bacterial diseases such as ascites disease, enteritis disease, eperythrozoonosis, fin rot disease simultaneously.Its cure rate can reach more than 80%, and prevention and therapeutic effect are remarkable.Can reduce and substitute antibiotic use, have important function improving aquatic product quality.This compound recipe is easy to use, cost is low, respond well, be easy to promote the use of, provide technical support to turbot and even Flounder Pleuronectidae class healthy aquaculture.
Claims (1)
1. the multiple-effect Chinese herb compound of a bacterial diseases of turbot is characterized in that its ingredient is: Cortex Phellodendri, Fructus Forsythiae, Flos Caryophylli, Radix Bupleuri, Radix Glycyrrhizae; Medicine is by weight ratio: 2: 1: 1: 1: 1; It is Powdered that medicine is, medical herbs granularity 200 orders.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110887A (en) * | 2013-01-21 | 2013-05-22 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting vibrio anguillarum |
| CN103110751A (en) * | 2013-01-21 | 2013-05-22 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting Edwardsiella tarda |
| CN104587317A (en) * | 2015-01-08 | 2015-05-06 | 青岛农业大学 | Traditional Chinese medicine composition for treating viral reddish body syndrome |
| CN105194041A (en) * | 2015-11-11 | 2015-12-30 | 郑州后羿制药有限公司 | Chinese herbal medicinal fermented extract liquid for preventing and treating bacterial diseases of freshwater fish and preparation method of Chinese herbal medicinal fermented extract liquid |
| CN105410481A (en) * | 2015-12-22 | 2016-03-23 | 青岛海之源智能技术有限公司 | Feed additive for preventing and treating turbot fin rot and preparation method thereof |
| CN108815279A (en) * | 2018-08-09 | 2018-11-16 | 集美大学 | It is a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio fluvialis disease |
| CN114949083A (en) * | 2022-06-16 | 2022-08-30 | 河北科技师范学院 | Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101524430A (en) * | 2008-12-17 | 2009-09-09 | 唐小阶 | Traditional Chinese fish drug |
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2010
- 2010-01-26 CN CN2010101007828A patent/CN101744924B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110887A (en) * | 2013-01-21 | 2013-05-22 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting vibrio anguillarum |
| CN103110751A (en) * | 2013-01-21 | 2013-05-22 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting Edwardsiella tarda |
| CN103110751B (en) * | 2013-01-21 | 2014-11-05 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting Edwardsiella tarda |
| CN103110887B (en) * | 2013-01-21 | 2014-12-10 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting vibrio anguillarum |
| CN104587317A (en) * | 2015-01-08 | 2015-05-06 | 青岛农业大学 | Traditional Chinese medicine composition for treating viral reddish body syndrome |
| CN104587317B (en) * | 2015-01-08 | 2017-08-08 | 青岛农业大学 | A kind of Chinese medicine composition for treating turbot reddish body |
| CN105194041A (en) * | 2015-11-11 | 2015-12-30 | 郑州后羿制药有限公司 | Chinese herbal medicinal fermented extract liquid for preventing and treating bacterial diseases of freshwater fish and preparation method of Chinese herbal medicinal fermented extract liquid |
| CN105410481A (en) * | 2015-12-22 | 2016-03-23 | 青岛海之源智能技术有限公司 | Feed additive for preventing and treating turbot fin rot and preparation method thereof |
| CN108815279A (en) * | 2018-08-09 | 2018-11-16 | 集美大学 | It is a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio fluvialis disease |
| CN114949083A (en) * | 2022-06-16 | 2022-08-30 | 河北科技师范学院 | Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder |
| CN114949083B (en) * | 2022-06-16 | 2023-08-04 | 秦皇岛粮丰海洋生态科技开发股份有限公司 | Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder |
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| CN101744924B (en) | 2011-10-05 |
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