CN114949083B - Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder - Google Patents

Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder Download PDF

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CN114949083B
CN114949083B CN202210687892.1A CN202210687892A CN114949083B CN 114949083 B CN114949083 B CN 114949083B CN 202210687892 A CN202210687892 A CN 202210687892A CN 114949083 B CN114949083 B CN 114949083B
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chinese herbal
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medicine composition
flounder
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CN114949083A (en
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高桂生
史秋梅
任海
靳晓敏
赵奇
韩雅静
韩红升
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Qinhuangdao Liangfeng Marine Ecology Science & Technology Development Co ltd
Hebei Normal University of Science and Technology
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Hebei Normal University of Science and Technology
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/85—Verbenaceae (Verbena family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/739—Sanguisorba (burnet)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
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Abstract

The invention discloses a Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder. Belongs to the technical field of Chinese herbal medicines. The Chinese herbal medicine composition comprises the following components: 3-5 parts of beautyberry, 3-5 parts of humifuse euphorbia herb, 3-5 parts of Chinese brake herb, 1-2 parts of longan seed, 1-2 parts of garden burnet root charcoal and 1-2 parts of liquorice. The Chinese herbal medicine composition is screened according to the traditional ancient formula of medicine and combining with the theory of fish physiology, infectious disease etiology and immunology on the basis of a large number of practical applications. The Chinese herbal medicine composition can fully exert the effects of the raw materials in the components, is used for treating the main bacterial diseases of the flounder, and has the advantages of short treatment course, low treatment cost, high cure rate, safety, no toxic or side effect and convenient use.

Description

Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder
Technical Field
The invention relates to the technical field of Chinese herbal medicines, in particular to a Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder.
Background
The flounder mainly comprises cynoglossus semilaevis, turbot, paralichthys olivaceus and the like, and the main bacterial diseases comprise bacterial septicemia, enteritis, ascites, tail rot, skin ulcer and the like. The pathogenic bacteria mainly comprise Edwardsiella tarda, vibrio vulnificus, vibrio alginolyticus, vibrio parahaemolyticus, vibrio anguillarum, etc.
At present, antibiotics and chemical medicines are mainly used for preventing and treating the bacterial diseases, but long-term use of the antibiotics and the chemical medicines not only pollute the water environment, but also cause re-injury to fish bodies, form medicine residues, and make diseases more difficult to control.
The Chinese herbal medicine has the characteristics of nature, small toxic and side effects, difficult generation of drug resistance and the like, is used for preventing and treating diseases of flounder and has wide application prospect. Although the prior art discloses a Chinese herbal medicine preparation for preventing and treating bacterial diseases of flounder, the problems of high cost, poor treatment effect and the like exist.
In summary, how to provide a Chinese herbal medicine preparation with low cost and good treatment effect for preventing and treating bacterial diseases of flounder is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of this, the present invention provides a Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder comprises the following components: 3-5 parts of beautyberry, 3-5 parts of humifuse euphorbia herb, 3-5 parts of Chinese brake herb, 1-2 parts of longan seed, 1-2 parts of garden burnet root charcoal and 1-2 parts of liquorice.
The pharmacological effects of the components in the Chinese herbal medicine composition are as follows:
efficacy of Callicarpa nudiflora: the beautyberry is also called festival red, rice soup leaf, etc. Neutral nature and bitter taste, enter lung, large intestine, liver and spleen meridians. The whole herb water decoction can shorten the bleeding time of rabbits and has the function of hemostasis; the herb decoction has good antibacterial effect on staphylococcus aureus, edwardsiella tarda and vibrio vulnificus, and can promote phagocytosis of leucocytes. Has antiinflammatory, toxic materials removing, astringent, and hemostatic effects. Can be used for treating traumatic hemorrhage, gastrointestinal hemorrhage, etc.
Humifuse euphorbia herb efficacy: herba Euphorbiae Humifusae alias herba Chenopodii, herba Erigerontis, etc. Pungent and neutral in flavor, enters lung, liver, stomach, large intestine and bladder meridians. Comprises gallic acid, gallic acid methyl ester, quercetin, inositol, tannic acid, etc. The herba Euphorbiae Humifusae fresh juice, decoction and water decoction concentrated ethanol extract have inhibiting effect on various cocci, bacilli, vibrio and the like. Has effects of clearing heat and detoxicating, promoting diuresis and eliminating jaundice, promoting blood circulation and stopping bleeding. Can be used for treating dysentery, diarrhea, hematochezia, trauma, and pyocutaneous disease due to heat toxin.
Pteris multifida efficacy: herba Pteridis Multifidae alias herba Pteridis Multifidae, radix seu herba Pteridis Multifidae, and herba Pteridis Multifidae. Light in nature, slightly bitter in taste, cool, enter liver, kidney and large intestine meridians. Contains vitexin, syringic acid, protocatechuic aldehyde and protocatechuic acid. Has obvious inhibiting effect on various cocci, bacilli, vibrio and the like. Has effects of clearing heat, promoting diuresis, removing toxic substances, relieving dysentery, cooling blood, and stopping bleeding. Can be used for treating dysentery, gastroenteritis, traumatic hemorrhage, etc.
Longan seed efficacy: longan seeds are called longan seeds respectively, and they are cold in nature, sour and astringent in taste, and enter lung, large intestine and kidney meridians. Contains saporin and fat. Has hemostatic, analgesic, qi regulating, and dampness eliminating effects. Can be used for treating traumatic hemorrhage, scabies, and skin sore.
The garden burnet charcoal has the effects: the garden burnet root charcoal is Huang Zhaoxiang, the mountain sweet potato, the pig ginseng, the sanguinaria, and the like, and is cool and bitter in taste. Enter liver, stomach and large intestine meridians. Contains various tannin components. Has effects in stopping bleeding, cooling blood, clearing away heat and toxic materials, astringing, relieving diarrhea, and inhibiting various pathogenic microorganisms. Can be used for treating bloody dysentery, gastrointestinal hemorrhage, ulcer hemorrhage, hematochezia, etc.
Licorice efficacy: liquorice aliases Meicao, miao, lao, sweet grass, sweet root, etc. Neutral nature and sweet taste, enter spleen, stomach and lung meridians. Comprises glycyrrhizic acid, glycyrrhizin, licoflavone, glycyrrhizin, isoliquiritigenin, and Glycyrrhrizae radix polysaccharide, and has antiulcer and macrophage phagocytic function regulating effects. Glycyrrhizic acid is a natural antidote and can be used for enhancing immunity, maintaining water and salt metabolism balance, etc. Has effects of invigorating spleen, replenishing qi, clearing heat, detoxicating, relieving spasm, relieving pain, and harmonizing various drugs. Can be used for treating weakness of spleen and stomach, asthenia, carbuncle, skin sore, and toxic materials, and for relieving virulence.
The formula of the invention is as follows: the beautyberry, the humifuse euphorbia herb and the phoenix-tail fern have the effects of clearing heat and detoxicating, resisting bacteria and diminishing inflammation, cooling blood and stopping bleeding, have a wide antibacterial spectrum, and are effective on various gram-positive bacteria and gram-negative bacteria; herba Euphorbiae Humifusae and herba Pteridis Multifidae can stop chronic dysentery, thicken intestines and stomach, and regulate middle-jiao and invigorate spleen; longan seeds and garden burnet are bitter and cool, purge fire and detoxify, astringe and promote granulation, and liquorice regulates the body immunity function, gan Wenyi qi, passes channels and collaterals and harmonizes the medicines. The Chinese medicinal materials are combined to play the roles of resisting bacteria, diminishing inflammation, relieving diarrhea, stopping dysentery, stopping bleeding, promoting granulation, improving the immunity of the organism and improving the disease resistance. Is suitable for preventing and treating bacterial diseases of flounder.
Further, a Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder comprises the following components: 5 parts of beautyberry, 5 parts of humifuse euphorbia herb, 5 parts of phoenix-tail fern herb, 2 parts of longan seeds, 2 parts of garden burnet root charcoal and 1 part of liquorice.
Further, the bacterial disease is bacterial septicemia, enteritis, ascites, tail rot or skin ulcer.
Compared with the prior art, the invention has the beneficial effects that: the Chinese herbal medicine composition is screened according to the traditional ancient formula of medicine and combining with the theory of fish physiology, infectious disease etiology and immunology on the basis of a large number of practical applications. The Chinese herbal medicine composition can fully exert the effects of the raw materials in the components, is used for treating the main bacterial diseases of the flounder, and has the advantages of short treatment course, low treatment cost, high cure rate, safety, no toxic or side effect and convenient use.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The required medicament is a conventional experimental medicament and is purchased from a commercial channel; the test methods not mentioned are conventional test methods and will not be described in detail herein.
Example 1
1. Respectively weighing 250g of beautyberry, 250g of humifuse euphorbia herb, 250g of Chinese brake herb, 100g of longan seed, 100g of garden burnet root charcoal and 50g of liquorice.
2. Pulverizing the above materials into coarse powder (sieving with 40-60 mesh sieve), respectively.
3. Soaking the coarse powder of the six Chinese herbal medicines in 8000mL of water for 1 hour, decocting, boiling water, and decocting with slow fire for 30 minutes; filtering to obtain primary filtrate, decocting the residue twice with 6000mL water for 20min, and filtering to obtain secondary and tertiary filtrates; combining the three filtrates, and concentrating to 900mL to obtain concentrated solution.
4. Adding sodium benzoate with the mass concentration of 0.05% as a preservative into the obtained concentrated solution, adding water to a volume of 1000mL, stirring uniformly, filtering, filling and sterilizing to obtain the Chinese herbal medicine composition liquid.
Example 2
In the step 1, 150g of beautyberry, 150g of humifuse euphorbia herb, 150g of Chinese brake herb, 50g of longan seed, 50g of garden burnet root charcoal and 50g of liquorice are weighed.
The rest of the procedure is the same as in example 1.
Example 3
In the step 1, 250g of beautyberry, 250g of humifuse euphorbia herb, 200g of Chinese brake herb, 100g of longan seed, 100g of garden burnet root charcoal and 100g of liquorice are weighed.
The rest of the procedure is the same as in example 1.
Some of the test cases performed using the herbal composition of the present invention are as follows:
the Chinese herbal medicine composition (5 parts of beautyberry, 5 parts of humifuse euphorbia herb, 5 parts of Chinese brake herb, 2 parts of longan seed, 2 parts of garden burnet root charcoal and 1 part of liquorice) is tested by taking the bacterial septicemia for preventing and treating the vibrio vulnificus as an example.
Example 4
(1) Measuring the index:
the Chinese herbal medicine composition disclosed by the invention is used for measuring the bacteriostasis diameter, the minimum bacteriostasis concentration and the minimum bacteriostasis concentration of vibrio vulnificus.
(2) The measuring method comprises the following steps:
(1) antibacterial diameter: measuring the drug-sensitive diameter by adopting a paper sheet agar diffusion method, wherein the drug content of each drug-sensitive paper sheet of the tested Chinese herbal medicines and the Chinese herbal medicine composition is 5mg, 10mg and 20mg, taking out the prepared drug-sensitive paper sheet by using sterile forceps through aseptic operation, and pasting the prepared drug-sensitive paper sheet on vibrio vulnificus (bacterial solution concentration is 5.5 mm 10) 4 CFU), the test for each tablet was repeated 3 times. 28 DEG CAfter culturing in a constant temperature incubator for 24 hours, the results were observed.
(2) Minimum inhibitory concentration: the concentration of the tube is the lowest antibacterial concentration of the medicine on the strain when no bacteria grow in the tube with the highest dilution of the medicine.
(3) Minimum bactericidal concentration: on the basis of the minimum inhibitory concentration test, the materials of each tube without bacterial growth are respectively coated on an agar plate culture medium, and after the culture is carried out for 24 to 48 hours in a constant temperature incubator at 28 ℃, the result is observed. The agar plate has no bacteria growth and contains 1 tube with minimum liquid medicine, namely the lowest sterilizing concentration of the medicine to the strain.
The antibacterial diameter, the lowest antibacterial concentration and the lowest antibacterial concentration of the Chinese herbal medicine composition for vibrio vulnificus are shown in table 1.
Table 1 Chinese herbal medicine composition has antibacterial diameter, lowest antibacterial concentration and lowest antibacterial concentration for Vibrio vulnificus
The results show that: each single Chinese herbal medicine has a certain antibacterial effect, the antibacterial diameter increases along with the increase of the concentration of the medicine, wherein the humifuse euphorbia herb, the Chinese brake herb and the garden burnet root have better antibacterial effect, and the callicarpa nudiflora, the longan seeds and the liquorice have a certain antibacterial effect. Compared with the single Chinese herbal medicines, the Chinese herbal medicine composition has the highest antibacterial diameter, and the antibacterial diameter of a paper sheet group with the dosage of 20mg reaches 22.5mm. The antibacterial diameters of the Chinese herbal medicine composition paper sheets of the invention, which contain 5mg, 10mg and 20mg of the medicines, on vibrio vulnificus are 16.5mm, 18.0mm and 22.5mm respectively. The Chinese herbal medicine composition can inhibit the reproduction and growth of pathogen vibrio vulnificus and can also directly kill pathogen vibrio vulnificus. The minimum inhibitory concentration of the Chinese herbal medicine composition is 1.75mg/mL, the minimum bactericidal concentration is 1.90mg/mL, and compared with the single Chinese herbal medicines, the minimum inhibitory concentration and the minimum bactericidal concentration are the lowest in numerical value, and the effect is the best, which indicates that compatibility of six Chinese herbal medicines of 'callicarpa nudiflora, humifuse euphorbia herb, chinese brake herb, longan seed, garden burnet root charcoal' in the Chinese herbal medicine composition has a synergistic effect.
Example 3
The invention relates to a drug effect test of a Chinese herbal medicine composition for artificially infecting vibrio vulnificus with cynoglossus semilaevis, which comprises the following steps:
after a few days of temporary rearing of cynoglossus semilaevis, 0.2mL of Vibrio vulnificus suspension (bacterial liquid concentration 5.5 x 10) is injected into the chest fin of each fish 4 CFU). The Chinese herbal medicine composition liquid prepared in the example 1 is uniformly sprayed on columnar baits according to the feed dosage of 0.5%, 1% and 1.5% in the first half hour of feeding fish, and the feeding rate is 1.8% of the weight of the tested fish after 2-3 times of daily feeding. After 7d of baits are continuously fed, the cure condition of the cynoglossus semilaevis is observed.
(1) Measuring the index: mortality rate and cure rate.
(2) And (3) a statistical formula:
the test results of the efficacy of the Chinese herbal medicine composition of the invention on vibrio vulnificus infected with cynoglossus semilaevis are shown in table 2.
TABLE 2 results of the test of the efficacy of the Chinese herbal composition of the present invention against Vibrio vulnificus infection of Cynoglossus semilaevis
The results show that: the death rate of the cynoglossus semilaevis is highest and is 80% when the Chinese herbal medicine composition is not added, most of dead fishes in the test show typical bacterial septicemia symptoms, and the fish body shows bleeding, protruding anus and rotten tail; the mortality of the cynoglossus semilaevis added with the Chinese herbal medicine composition of the invention is 15%, 8% and 12%, and the cure rate is 85%, 92% and 88% respectively. The bait in the test is prepared by adopting a full-drug adding mode, the difference of the control effect of the bait of the Chinese herbal medicine composition of the invention between the group of 0.5 percent and the group of 1.0 percent is not obvious, but the dosage of the Chinese herbal medicine composition of the invention of the group of 1.5 percent has too heavy drug flavor, influences the smell and the taste of the cynoglossus semilaevis, reduces the ingestion rate, reduces the control effect, has the cure rate of only 88 percent, and increases the medication cost. Therefore, it is suggested to prepare the pharmaceutical baits in a dosage of 1.0% of the group of the Chinese herbal medicine composition of the present invention in production.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (3)

1. The Chinese herbal medicine composition for preventing and treating the bacterial diseases of the flounder is characterized by comprising the following components in parts by weight: 3-5 parts of beautyberry, 3-5 parts of humifuse euphorbia herb, 3-5 parts of Chinese brake herb, 1-2 parts of longan seed, 1-2 parts of garden burnet root charcoal and 1-2 parts of liquorice.
2. The Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder according to claim 1, which is characterized by comprising the following components in parts by weight: 5 parts of beautyberry, 5 parts of humifuse euphorbia herb, 5 parts of phoenix-tail fern herb, 2 parts of longan seeds, 2 parts of garden burnet root charcoal and 1 part of liquorice.
3. The herbal composition according to any one of claims 1 or 2, wherein the bacterial disease is bacterial sepsis, enteritis, ascites, tail rot or skin ulcer.
CN202210687892.1A 2022-06-16 2022-06-16 Chinese herbal medicine composition for preventing and treating bacterial diseases of flounder Active CN114949083B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101744924A (en) * 2010-01-26 2010-06-23 中国水产科学研究院黄海水产研究所 Multiple-effect Chinese herbal medicine compound of bacterial diseases of turbot
CN106266138A (en) * 2016-09-30 2017-01-04 威海虹润海洋科技有限公司 A kind of Chinese herbal and crude drugs preparations for preventing and treating industrialized culture left-eyed flounder Disease of skin fester

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101744924A (en) * 2010-01-26 2010-06-23 中国水产科学研究院黄海水产研究所 Multiple-effect Chinese herbal medicine compound of bacterial diseases of turbot
CN106266138A (en) * 2016-09-30 2017-01-04 威海虹润海洋科技有限公司 A kind of Chinese herbal and crude drugs preparations for preventing and treating industrialized culture left-eyed flounder Disease of skin fester

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
中草药(Chinese herb medicine)在水产动物疾病防治中的应用与展望;柳富荣;现代渔业信息(第07期);17-20 *

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