CN101679990B - △8去饱和酶及其在制备多不饱和脂肪酸中的用途 - Google Patents

△8去饱和酶及其在制备多不饱和脂肪酸中的用途 Download PDF

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CN101679990B
CN101679990B CN2008800187631A CN200880018763A CN101679990B CN 101679990 B CN101679990 B CN 101679990B CN 2008800187631 A CN2008800187631 A CN 2008800187631A CN 200880018763 A CN200880018763 A CN 200880018763A CN 101679990 B CN101679990 B CN 101679990B
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seq
desaturase
acid
sequence
host cell
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CN101679990A (zh
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H·G·达穆德
Q·Q·朱
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
CN2008800187631A 2007-04-10 2008-04-10 △8去饱和酶及其在制备多不饱和脂肪酸中的用途 Expired - Fee Related CN101679990B (zh)

Applications Claiming Priority (3)

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US91083107P 2007-04-10 2007-04-10
US60/910,831 2007-04-10
PCT/US2008/004700 WO2008124194A1 (en) 2007-04-10 2008-04-10 Delta-8 desaturases and their use in making polyunsaturated fatty acids

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CN101679990A CN101679990A (zh) 2010-03-24
CN101679990B true CN101679990B (zh) 2013-06-19

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US (5) US8188338B2 (enExample)
EP (2) EP2132317B1 (enExample)
JP (1) JP5501952B2 (enExample)
CN (1) CN101679990B (enExample)
AU (2) AU2008237065A1 (enExample)
BR (1) BRPI0808222A2 (enExample)
CA (2) CA2683497C (enExample)
DK (1) DK2132317T3 (enExample)
WO (2) WO2008124806A2 (enExample)

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WO2010147907A1 (en) 2009-06-16 2010-12-23 E. I. Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica
AU2010317140B2 (en) 2009-11-03 2016-04-21 Dsm Ip Assets B.V. Vegatable oil comprising a polyunsaturaded fatty acid having at least 20 carbon atoms
JP6476436B2 (ja) 2009-11-03 2019-03-06 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 細胞および少なくとも２０個の炭素原子を有する多価不飽和脂肪酸（ｌｃ−ｐｕｆａ）を含んでなる組成物
CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
US8703473B2 (en) 2010-08-26 2014-04-22 E I Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
WO2012027676A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
AU2011293189B2 (en) 2010-08-26 2017-02-16 E. I. Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
BR112013016769A2 (pt) 2010-12-30 2016-10-11 Du Pont yarrowia lipolytica transformada e método para produzir pelo menos um produto não nativo de interesse
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA2892516A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
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EA030896B1 (ru) 2013-08-16 2018-10-31 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
EP3692786B1 (en) 2013-09-13 2022-11-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2015120276A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International Inc Insecticidal proteins and methods for their use
US10480007B2 (en) 2014-02-07 2019-11-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants
US20170247719A1 (en) 2014-09-17 2017-08-31 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2016061206A1 (en) 2014-10-16 2016-04-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX369475B (es) 2015-01-15 2019-11-08 Pioneer Hi Bred Int Proteinas insecticidas y metodos para sus usos.
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US10647995B2 (en) 2015-06-16 2020-05-12 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
EP3943602A1 (en) 2015-08-06 2022-01-26 Pioneer Hi-Bred International, Inc. Plant derived insecticidal proteins and methods for their use
EP3341483B1 (en) 2015-08-28 2019-12-18 Pioneer Hi-Bred International, Inc. Ochrobactrum-mediated transformation of plants
CN105137844B (zh) * 2015-08-31 2017-09-01 广东电网有限责任公司电力科学研究院 锅炉调试过程中风险预控方法和系统
EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3451837B1 (en) 2016-05-04 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20190185867A1 (en) 2016-06-16 2019-06-20 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN109788735A (zh) 2016-07-01 2019-05-21 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
US20200015500A1 (en) 2016-12-15 2020-01-16 Dsm Ip Assets B.V. Blend formulation comprising silicate and microbial and / or plant cells comprising a polyunsaturated fatty acid having at least 20 carbon atoms (lc-pufa)
CN111867377B (zh) 2018-03-14 2023-05-23 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
MX2020009435A (es) 2018-03-14 2020-11-11 Pioneer Hi Bred Int Proteinas con actividad insecticida de plantas y metodos para sus usos.
US12168774B2 (en) 2020-07-14 2024-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use

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US20100267099A1 (en) 2010-10-21
US8338152B2 (en) 2012-12-25
AU2008236595B2 (en) 2013-06-13
EP2121737B1 (en) 2015-05-27
WO2008124806A2 (en) 2008-10-16
US20080254192A1 (en) 2008-10-16
US20080254521A1 (en) 2008-10-16
EP2132317B1 (en) 2013-02-27
CA2681145C (en) 2016-02-16
BRPI0808222A2 (pt) 2014-07-08
JP2010523147A (ja) 2010-07-15
US7790156B2 (en) 2010-09-07
DK2132317T3 (da) 2013-06-03
US20120196347A1 (en) 2012-08-02
CN101679990A (zh) 2010-03-24
AU2008237065A1 (en) 2008-10-16
WO2008124806A3 (en) 2010-07-22
CA2681145A1 (en) 2008-10-16
US8318463B2 (en) 2012-11-27
JP5501952B2 (ja) 2014-05-28
US8188338B2 (en) 2012-05-29
CA2683497C (en) 2015-08-11
US20100099900A1 (en) 2010-04-22
EP2121737A2 (en) 2009-11-25
CA2683497A1 (en) 2008-10-16
AU2008236595A1 (en) 2008-10-16
WO2008124194A1 (en) 2008-10-16
EP2132317A1 (en) 2009-12-16

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