CN101638622A - Microbial inoculum for degrading straw - Google Patents
Microbial inoculum for degrading straw Download PDFInfo
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- CN101638622A CN101638622A CN 200810224244 CN200810224244A CN101638622A CN 101638622 A CN101638622 A CN 101638622A CN 200810224244 CN200810224244 CN 200810224244 CN 200810224244 A CN200810224244 A CN 200810224244A CN 101638622 A CN101638622 A CN 101638622A
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Abstract
The invention discloses a microbial inoculum for degrading straw and the active ingredients of the microbial inoculum comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratio of Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The microbial inoculumfor degrading straw of the invention can mature straw fast to prepare organic fertilizer, and the prepared organic fertilizer can be uses as a base fertilizer and can obviously improve the quality ofvegetables.
Description
Technical field
The present invention relates to a kind of microbial inoculum that is used for degrading straw.
Background technology
Agricultural crop straw is the non-competing resource of a kind of potential, have quantity big, distribute wide and characteristics such as kind is many.It is reported that China's all kinds of agricultural crop straw gross annual output amount reaches more than 700,000,000 tons, wherein straw is 2.3 hundred million tons, 2.2 hundred million tons of maize straws, and 1,000,000,000 tons of beans and autumn coarse cereals stalks, 1.0 hundred million tons of peanut, potato class tendril and beet leavess etc. account for about 30% of the total stalk amount in the world.But these stalk resources are not but reasonably developed for a long time, and traditionally, stalk is used as the roughage that peasant household produces fuel or is used for livestock, is used for papermaking on a small quantity.In recent years, because peasant household's life fuel falls sharply, straw directly returning to field or become thoroughly decomposed that also the field amount is few, and directly burning amount of field is big, the whole nation is annual to have 2/3 stalk directly to be burned approximately.Only account for 2.6% through what utilize after the technical finesse in all stalks.
Carbon accounts for the overwhelming majority in the stalk, is potassium, silicon, calcium, magnesium, sulphur, phosphorus secondly, and the product after the burning mainly contains CO
2, CO, SO
2, NO
2And N
2O, these obnoxious flavoures have caused the serious environmental pollution after entering atmosphere.If the field is gone back in these rotten backs of stalk heap,, improves soil property with becoming the fertilizer fertilizing soil of horn of plenty.
Under the state of nature, cellulose degradation is mainly by three kinds of modes:
1) physics mode comprises pulverizing, boiling, heat spray etc.; 2) chemical mode comprises acidic hydrolysis, alkaline hydrolysis, ammonification and oxidative degradation; 3) microbiological deterioration.Physics or chemical process are handled stalk, can only slightly improve the digestibility of hemicellulose, and be little to the effect of Mierocrystalline cellulose and xylogen, fails to improve the nutritive value and the digestibility of stalk.Chemical process can be destroyed xylogen and combine with ester bond between the polysaccharide, make Mierocrystalline cellulose, hemicellulose and lignin separation, insoluble xylogen is become more diffluent hydroxyl xylogen, make the stalk structure become loose, crystalline cellulose becomes unformed Mierocrystalline cellulose, and then makes that stalk is easy to decompose.But the shortcoming of chemical process is: content of cellulose is too high, protein content low (about 5%-10%); The content of mineral substances imbalance, the A that is deficient in vitamin, vitamins D and vitamin-E etc.; Low (Qian Huiyue, 2004 of waiting of Energy value; Yang Lianyu, 2001).Microorganism is by being secreted into the outer free cellulose enzyme of born of the same parents, with lytic enzyme mechanism and oxydase mechanisms of degradation Mierocrystalline cellulose (Zhang Ying, 2005) to cellulosic degraded.Utilize microorganism Mierocrystalline cellulose finally can be decomposed into carbonic acid gas and water, and can produce a large amount of tropinas by the growth of microorganism self, therefore use microbial method to handle stalk, can increase substantially its nutritive value, compare with physico-chemical process, have many unrivaled advantages.
Productive rate and the activity of utilizing hybrid bacterial strain fermentation can raising cellulase have been reported the seventies in last century.In China, utilize the hybrid bacterial strain fermentation to improve the productive rate and active investigator's the extensive concern that also causes of cellulase.
Summary of the invention
The purpose of this invention is to provide a kind of microbial inoculum that is used for degrading straw.
The microbial inoculum that is used for degrading straw provided by the present invention, its active ingredient comprise viride (Trichoderma viride), yellow green trichoderma (Trichoderma aureoviride) and feed series bacillus (Paenibacillus pabuli).
The active ingredient that is used for the microbial inoculum of degrading straw provided by the present invention also can only have by described viride (Trichoderma viride), yellow green trichoderma (Trichoderma aureoviride) and feed series bacillus (Paenibacillus pabuli) to be formed.
Wherein, in the described microbial inoculum, the colony forming unit number of viride (Trichoderma viride), yellow green trichoderma (Trichodermaaureoviride) and feed series bacillus (Paenibacillus pabuli) is than being (0.8-1.2): (0.8-1.2): (0.8-1.2).
Described viride (Trichoderma viride) specifically can be viride (Trichoderma viride) ACCC 30166, described yellow green trichoderma (Trichoderma aureo viride) specifically can be yellow green trichoderma (Trichoderma aureoviride) ACCC 32248, and described feed series bacillus (Paenibacilluspabuli) specifically can be feed series bacillus (Paenibacillus pabuli) ACCC 10273.
In the microbial inoculum of described degrading straw, described viride (Trichoderma viride) ACCC 30166, described yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 and described feed series bacillus (Paenibacillus pabuli) ACCC 10273 can distinguish independent packaging, also can mix.
The microbial inoculum that is used for degrading straw of the present invention can be liquid preparation, also can be solid preparation.In liquid preparation, add sorbent material and can obtain solid preparation, as adding the light calcium carbonate or the peat composed of rotten mosses etc.
Agricultural crop straws such as the microbial inoculum that is used for degrading straw of the present invention can the quick composting corn, wheat, paddy rice prepared organic fertilizer, than the rotten fermentation time of normal heap 15 days at least in advance.Use alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium and organic content, soil urease liveness and cellulase activity in the soil of organic fertilizer of preparation all than direct use stalk with do not use the soil height of fertilizer, the organic fertilizer of the present invention's preparation is as base manure, can significantly improve the quality of vegetables, use the rape and the Chinese cabbage of this organic fertilizer, its chlorophyll content can maintain higher level, compare with Chinese cabbage with the rape of directly using stalk, growth rape in mid-term and Chinese cabbage chlorophyll content improve 25.99% and 10.98% respectively; Can promote simultaneously rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, compare with Chinese cabbage that rape and Chinese cabbage output improve 22.97% and 2.80% respectively with the rape of directly using stalk; And rape reducing sugar and Vc content improve 21.8% and 6.89% respectively; Chinese cabbage reducing sugar and Vc content improve 9% and 23.53% respectively.
Embodiment
The used bacterial classification of following embodiment comes from INST OF AGRICULTURAL RESOURCES Chinese agriculture microbial strains preservation administrative center and (is called for short ACCC, address: No.12 ,zhongguancun south street,Haidian District, Beijing, INST OF AGRICULTURAL RESOURCES, postcode 100081).
Embodiment 1, be used for the preparation of the microbial inoculum of degrading straw
The microbial inoculum that is used for degrading straw is made up of reagent A, reagent B and reagent H, reagent A, reagent B and reagent H independent packaging.Reagent A, reagent B and reagent H are prepared as follows:
Strain fermentation substratum: Semen Maydis powder 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, 4ml/100ml Vogel ' s mother liquor, pH value 8.5.
Viride (Trichoderma viride) ACCC 30166, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent A.
Yellow green trichoderma (Trichoderma aureoviride) ACCC 32248, in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent B.
Feed series bacillus (Bacillus paenibacillus pabuli) ACCC 10273 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent H.
Viride in the reagent A (Trichoderma viride) ACCC 30166, the colony forming unit number ratio of feed series bacillus (Paenibacillus pabuli) is 1: 1: 1 among (Trichoderma aureoviride) ACCC 32248 of yellow green trichoderma among the reagent B and the reagent H.
2) cellulase activity, filter paper enzyme activity and the beta-glucosidase that is used for the microbial inoculum of degrading straw lived
Mensuration is used for microbial inoculum cellulase activity, filter paper enzyme activity and the beta-glucosidase of degrading straw and lives.
It is as follows that the DNS reducing sugar method is measured the cellulase activating method: at first adopt 3, and 5-dinitrosalicylic acid (DNS), the 550nm place measures absorbancy, with dextrose anhydrous gradient solution drawing standard curve; (represent restriction endonuclease to live with CMC enzyme activity (CMCase activity), get 1.0% carboxymethylcellulose sodium solution of the acetum preparation of 1.5mL 0.05mol/L sodium-acetate, add the suitable dilution of 0.5mL and fall the microbial inoculum that is used to separate stalk, add 3mL DNS reagent behind 50 ℃ of reaction 30min, boiling water bath 5min, cooling back thin up is surveyed reducing sugar to 25mL at 550nm.
Filter paper enzyme activity (filter paper activity, FPA) measuring method is as follows: the acetum of getting 1.5mL 0.05mol/L sodium-acetate adds the 1.5mL suitably microbial inoculum that is used for degrading straw and 1 WhatmanNo.1 filter paper bar (about 50mg of dilution, 1cm * 6cm), add 3mL DNS reagent behind 50 ℃ of insulation 60min, boiling water bath 5min, cooling back thin up is surveyed reducing sugar to 25mL at 550nm.
The measuring method of beta-glucoside enzyme activity is as follows: the 1.0% saligenin solution of getting the acetum preparation of 1.5mL 0.05mol/L sodium-acetate adds the suitably microbial inoculum that is used for degrading straw of dilution of 0.5mL, add 3mL DNS reagent behind 50 ℃ of reaction 30min, boiling water bath 5min, cooling back thin up is to 25mL, survey reducing sugar at 550nm, calculate enzyme numerical value alive according to the typical curve of drawing then.
Measurement result shows that the CMC enzyme (CMCA) alive that is used for the microbial inoculum of degrading straw reaches 3813.06U/mL, and filter paper enzyme activity (FPA) reaches 2107.57U/mL, and beta-glucosidase reaches 3227.17U/mL.
Above-mentioned experimental result illustrates the mutual supplement with each other's advantages that not only has FPA enzyme and beta-glucosidase between three bacterial strains, and reduced whole Mierocrystalline cellulose enzyme to the dependency of nutritive substance and the susceptibility of meta-bolites inhibition, make enzyme activity keep high level in a long time.
Embodiment 2, be used for the preparation of the microbial inoculum of degrading straw
The microbial inoculum that is used for degrading straw is made up of reagent A, reagent B and reagent H, and reagent A, reagent B and reagent H mix and make the microbial inoculum that is used for degrading straw.
Reagent A, reagent B and reagent H are prepared as follows:
Strain fermentation substratum: Semen Maydis powder 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, 4ml/100ml Vogel ' s mother liquor, pH value 8.5.
Viride (Trichoderma viride) ACCC 30166 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent A.
Yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent B.
Feed series bacillus (Paenibacillus pabuli) ACCC 10273 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 4d collects all fermented liquids as reagent H.
The microbial inoculum viride that is used for degrading straw (Trichoderma viride) ACCC 30166, yellow green trichoderma (Trichoderma aureoviride) ACCC32248 that makes after reagent A, reagent B and reagent H mix and the colony forming unit number ratio of feed series bacillus (Paenibacillus pabuli) ACCC 10273 are 0.8: 0.8: 1.2.
Embodiment 3, be used for the microbial inoculum of degrading straw
The microbial inoculum that is used for degrading straw is made up of reagent A, reagent B and reagent H, reagent A, reagent B and reagent H independent packaging respectively.Reagent A, reagent B and reagent H are prepared as follows:
Strain fermentation substratum: Semen Maydis powder 0.5g/L, corn stalk powder 15g/L, wheat bran 0.25g/L, soybean cake powder 0.25g/L, 4ml/100ml Vogel ' s mother liquor, pH value 8.5.
Viride (Trichoderma viride) ACCC 30166 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 5d collects all fermented liquids as reagent A.
Yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 5d collects all fermented liquids as reagent B.
Feed series bacillus (Paenibacillus pabuli) ACCC 10273 in the strain fermentation substratum 28 ℃, 200r/min shaking table shaking culture 3d collects all fermented liquids as reagent H.
The microbial inoculum viride that is used for degrading straw (Trichoderma viride) ACCC 30166, yellow green trichoderma (Trichoderma aureoviride) ACCC32248 that makes after reagent A, reagent B and reagent H mix and the colony forming unit number of feed series bacillus (Paenibacillus pabuli) ACCC 10273 were than 1.2: 1.2: 0.8.
The microbial inoculum degrading straw that is used for degrading straw of embodiment 4, embodiment 1 and embodiment 3 prepares straw organic fertilizer
1) preparation straw organic fertilizer
With maize straw disintegrating machine powder essence, the stalk fragment of acquisition is less than 1.0cm, as handled thing.Ratio according to 15kg urea/ton stalk in handled thing adds urea, according to 1 * 10
5Yellow green trichoderma (Trichodermaaureoviride) ACCC of cfu 32248 spores/kg stalk inserts the reagent B of embodiment 1; Then according to 1 * 10
5The reagent H that cfu feed series bacillus (Paenibacillus pabuli) ACCC 10273 spores/kg stalk inserts embodiment 1 fermented 1.5 days; At last, according to 1 * 10
5Cfu viride (Trichoderma viride) ACCC 30166 spores/kg stalk inserts the reagent A of embodiment 1, and fermentation 25d makes straw organic fertilizer 1.
With maize straw disintegrating machine powder essence, the stalk fragment of acquisition is less than 1.0cm, as handled thing.Ratio according to 15kg urea/ton stalk in handled thing adds urea, according to 1.5 * 10
4Yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 spores of cfu/kg stalk inserts the reagent B of embodiment 1; Then according to 1.0 * 10
5The reagent H that cfu feed series bacillus (Paenibacillus pabuli) ACCC 10273 spores/kg stalk inserts embodiment 1 fermented 2 days; At last according to 1.5 * 10
4Cfu viride (Trichodermaviride) ACCC 30166 spores/kg stalk inserts the reagent A of embodiment 1, and fermentation 20d makes straw organic fertilizer 3.
2) WSC of the organic fertilizer of step 1) preparation and total organic nitrogen are measured
The straw organic fertilizer total organic nitrogen is measured and is adopted H
2SO
4-H
2O
2Disappear and boil, Kjeldahl determination, straw organic fertilizer WSC (water-soluble carbon) are measured and are adopted potassium bichromate titrimetric method.Experiment repeats 3 times.
The measurement result of WSC and total organic nitrogen shows, the WSC of straw organic fertilizer 1 (water-soluble carbon) is 6.42g/kg, and the ratio of WSC and total organic nitrogen is 0.71, and T value (ratio of terminal point C/N and initial C/N) is 0.44, reach the requirement of becoming thoroughly decomposed, shift to an earlier date 15 days at least than the rotten fermentation time of normal heap; The WSC of straw organic fertilizer 3 (water-soluble carbon) is 5.82g/kg, and the ratio of WSC and total organic nitrogen is that 0.75T value (ratio of terminal point C/N and initial C/N) is 0.42, reaches the requirement of becoming thoroughly decomposed, and shifts to an earlier date 20 days at least than the rotten fermentation time of normal heap.
Above-mentioned numerical value is multiple mean value 3 times.
The microbial inoculum degrading straw that is used for degrading straw of embodiment 5, embodiment 2 prepares fertilizer
1) preparation organic fertilizer
With wheat stalk disintegrating machine powder essence, the stalk fragment of acquisition is less than 1.0cm, as handled thing.Ratio according to 15kg urea/ton stalk in handled thing adds urea, according to 1.0 * 10
4Yellow green trichoderma (Trichoderma aureoviride) ACCC 32248 spores of cfu/kg stalk or according to 1.5 * 10
4Cfu feed series bacillus (Paenibacillus pabuli) ACCC 10273 spores/kg stalk or according to 1.0 * 10
4Cfu viride (Trichoderma viride) ACCC 30166 spores/kg stalk inserts the bacteria fermentation 30d that is used for degrading straw of the preparation of embodiment 2, makes straw organic fertilizer 2.
2) WSC of the organic fertilizer of step 1) preparation and total organic nitrogen are measured
The WSC of straw organic fertilizer and total organic nitrogen measuring method are with embodiment 4.
The measurement result of WSC and total organic nitrogen shows, the WSC of organic fertilizer 2 (water-soluble carbon) is 6.40g/kg, and the ratio of WSC and total organic nitrogen is 0.70, and T value (ratio of terminal point C/N and initial C/N) is 0.48, reach the requirement of becoming thoroughly decomposed, shift to an earlier date 10 days at least than the rotten fermentation time of normal heap.
The field experiment of embodiment 6, straw organic fertilizer
Field experiment is selected rape and Chinese cabbage field respectively, adopt the completely random block design, 3 district's groups, each district's group is established 5 sub-districts, test group is that straw organic fertilizer 1,2 and 3 was used as base manure in sowing time or transplanting phase, contrast 1 is for directly using stalk, and contrast 2 is not for using any fertilizer, and every sub-district area is 15 square metres.
Contrast and test group except that apply fertilizer different, other field management are all identical.
After transplanting 40 days, measure alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium and organic content and soil urease liveness and cellulase activity in the soil.
Measure the soil alkali-hydrolyzable nitrogen with the alkaline hydrolysis diffusion process; Use 0.5molL
-1NaHCO
3Method is measured soil quick-effective phosphor; Use dense H
2SO
4-K
2Cr
2O
4Volumetry-outer heating method is measured soil organic matter content; With DNS reduction colorimetric method for determining cellulase activity; With sodium phenylate colorimetric method for determining urease activity.
Measurement result shows, alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium are compared with contrast 2 with organic content and are improved 7.38 ± 1.54mg/kg, 8.52 ± 2.36mg/kg, 15.30 ± 5.60mg/kg respectively in the soil of use straw organic fertilizer 1,1.1g/kg ± 0.43, soil urease liveness is compared average raising 14.38 ± 1.35% with contrast 2, cellulase activity is compared average raising 103.61 ± 12.58% with contrast 2, and comparison is high by 49.23 ± 6.32% according to 1.
Alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium are compared with contrast 2 with organic content and are improved 7.08 ± 2.40mg/kg, 8.00 ± 1.58mg/kg, 13.25 ± 4.56mg/kg respectively in the soil of use straw organic fertilizer 2,1.31 ± 0.15mg/kg, soil urease liveness is compared average raising 15.00 ± 3.65% with contrast 2, cellulase activity is compared average raising 106.37 ± 5.84% with contrast 2, and comparison is high by 55.45 ± 7.56% according to 1.
Alkali-hydrolyzable nitrogen, rapid available phosphorus, potassium are compared with contrast 2 with organic content and are improved 6.23 ± 1.50mg/kg, 6.78 ± 1.84mg/kg, 10.34 ± 5.21mg/kg respectively in the soil of use straw organic fertilizer 3,1.76 ± 0.62mg/kg, soil urease liveness is compared average raising 16 ± 2.35% with contrast 2, cellulase activity is compared average raising 95.37 ± 13.42% with contrast 2, and comparison is high by 56.47 ± 9.65% according to 1.
Growing mid-term, measuring rape and Chinese cabbage chlorophyll; After transplanting 26 days, measure reducing sugar and the Vc content of rape and Chinese cabbage; Measure the output of rape and Chinese cabbage in harvesting time, the experiment triplicate.
Chlorophyll measuring method is as follows:
Get fresh rape and the cabbage leaf of removing vein respectively, shred and place 20ml tool plug graduated tube, add 1: 1 by volume blended mixed solution of ethanol and acetone, 30 ℃ of soaked overnight, measure the vat liquor absorbancy at 440nm, 644nm, 662nm place respectively, according to Ca=9.784 * D
662-0.990 * D
644, Cb=21.426 * D
644-4.650 * D
662, Ca+b=5.134 * D
662+ 20.436 * D
644, Cc=4.695 * D
440-0.268 * (Ca+Cb) waits formula to calculate chlorophyll content (mg/L).Calculate chlorophyll content (mg/L).
Reducing sugar test adopts anthracene copper colorimetry.
The Vc assay adopts the 2,6 dichlorophenol indophenol volumetry.
Experimental result shows that straw organic fertilizer 1 is manured into soil and can impels rape and Chinese cabbage chlorophyll content can maintain higher level, compares with contrast 1, and growth rape in mid-term and Chinese cabbage chlorophyll content improve 25.99 ± 2.36% and 10.98 ± 1.48% respectively; Straw organic fertilizer 1 is manured into soil and can promotes rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, and compares with contrast 2, and rape and Chinese cabbage output improve 22.97 ± 3.34% and 2.80 ± 1.03% respectively; Straw organic fertilizer 1 is manured into soil and contrasts 2 and compare, and rape reducing sugar and Vc content improve 21.8 ± 2.85% and 6.89 ± 1.52% respectively; Chinese cabbage reducing sugar and Vc content improve 9 ± 0.58% and 23.53 ± 3.59% respectively.
Straw organic fertilizer 2 is manured into soil and can impels rape and Chinese cabbage chlorophyll content can maintain higher level, compares with contrast 1, and growth rape in mid-term and Chinese cabbage chlorophyll content improve 26.45 ± 4.07% and 11.89 ± 2.36% respectively; Straw organic fertilizer 2 is manured into soil and can promotes rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, and compares with contrast 2, and rape and Chinese cabbage output improve 24.05 ± 1.29% and 3.25 ± 1.30% respectively; Straw organic fertilizer 2 is manured into soil and contrasts 2 and compare, and rape reducing sugar and Vc content improve 25.30 ± 2.87% and 8.03 ± 1.29% respectively; Chinese cabbage reducing sugar and Vc content improve 10.36 ± 1.32% and 25.36 ± 2.20% respectively.
Straw organic fertilizer 3 is manured into soil and can impels rape and Chinese cabbage chlorophyll content can maintain higher level, compares with contrast 1, and growth rape in mid-term and Chinese cabbage chlorophyll content improve 26.00 ± 2.40% and 11.28 ± 1.68% respectively; Straw organic fertilizer 3 is manured into soil and can promotes rape and Chinese cabbage to the absorbing of nitrogen, phosphorus, potassium, and compares with contrast 2, and rape and Chinese cabbage output improve 21.07 ± 2.34% and 1.80 ± 1.03% respectively; Straw organic fertilizer 3 is manured into soil and contrasts 2 and compare, and rape reducing sugar and Vc content improve 19.8 ± 3.85% and 7.69 ± 2.52% respectively; Chinese cabbage reducing sugar and Vc content improve 8.91 ± 1.58% and 20.53 ± 4.59% respectively.
Claims (5)
1, a kind of microbial inoculum that is used for degrading straw, its active ingredient comprise viride (Trichoderma viride), yellow green trichoderma (Trichoderma aureoviride) and feed series bacillus (Paenibacillus pabuli).
2, microbial inoculum according to claim 1, it is characterized in that: in the described microbial inoculum, the colony forming unit number of described viride (Trichoderma viride), described yellow green trichoderma (Trichoderma aureoviride) and described feed series bacillus (Paenibacillus pabuli) is than being (0.8-1.2): (0.8-1.2): (0.8-1.2).
3, microbial inoculum according to claim 1 and 2, it is characterized in that: described viride (Trichodermaviride) is viride (Trichoderma viride) ACCC 30166, described yellow green trichoderma (Trichodermaaureoviride) is yellow green trichoderma (Trichoderma aureoviride) ACCC 32248, and described feed series bacillus (Paenibacillus pabuli) is feed series bacillus (Paenibacillus pabuli) ACCC10273.
4, according to arbitrary described microbial inoculum in the claim 1 to 3, it is characterized in that: in the microbial inoculum of described degrading straw, described viride (Trichoderma viride) ACCC 30166, described yellow green trichoderma (Trichodermaaureoviride) ACCC 32248 and described feed series bacillus (Paenibacillus pabuli) ACCC 10273 independent packagings respectively.
5, according to arbitrary described microbial inoculum in the claim 1 to 3, it is characterized in that: in the microbial inoculum of described degrading straw, described viride (Trichoderma viride) ACCC 30166, described yellow green trichoderma (Trichodermaaureoviride) ACCC 32248 and described feed series bacillus (Paenibacillus pabuli) ACCC 10273 mix.
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| CN101885624A (en) * | 2010-07-16 | 2010-11-17 | 湖北金帝农业科技有限公司 | Decomposition maturing agent for degrading straw |
| CN101885624B (en) * | 2010-07-16 | 2012-09-19 | 湖北金帝农业科技有限公司 | Decomposition maturing agent for degrading straw |
| CN102690755A (en) * | 2012-06-01 | 2012-09-26 | 德州市元和农业科技开发有限责任公司 | Compound microbial bacterial preparation for degrading crop straw and preparation method and application of compound microbial bacterial preparation |
| CN104026369A (en) * | 2014-05-15 | 2014-09-10 | 陈凯 | Novel preparing method of fermented daily ration for piglets |
| CN104026368A (en) * | 2014-05-15 | 2014-09-10 | 陈凯 | New method for preparing fermentation feed with health-care function for cows and sheep |
| CN103960537A (en) * | 2014-05-15 | 2014-08-06 | 陈凯 | New preparation method of fermented ration particles for cows |
| CN103960535A (en) * | 2014-05-15 | 2014-08-06 | 陈凯 | New preparation method of fermented fodder particles for meat ducks |
| CN103960538A (en) * | 2014-05-15 | 2014-08-06 | 陈凯 | New preparation method of fermented fodder particles for sows |
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| CN103976184A (en) * | 2014-05-15 | 2014-08-13 | 陈凯 | Preparation method for novel fermented feed particles for gooses |
| CN103960539A (en) * | 2014-05-15 | 2014-08-06 | 陈凯 | New preparation method of fermented fodder for meat ducks |
| CN104026367A (en) * | 2014-05-15 | 2014-09-10 | 陈凯 | Novel preparation method for fermentation chicken ration particles |
| CN103960536A (en) * | 2014-05-15 | 2014-08-06 | 陈凯 | New preparation method of fermented rations for broiler chickens |
| CN104726385A (en) * | 2015-04-22 | 2015-06-24 | 湖南龙腾生物科技有限公司 | Strain with high cellulose enzyme activity and application and obtaining methods thereof |
| CN104726385B (en) * | 2015-04-22 | 2017-12-22 | 湖南龙腾生物科技有限公司 | Bacterial strain and its application and preparation method with high-cellulose enzymatic activity |
| CN105941485A (en) * | 2016-05-14 | 2016-09-21 | 尤馨雅 | Efficient garden herbicide |
| CN105969697A (en) * | 2016-06-30 | 2016-09-28 | 河海大学 | Compound bacterium agent for composting straw as well as preparation method and application thereof |
| CN105969697B (en) * | 2016-06-30 | 2019-12-10 | 河海大学 | Compound microbial inoculum for straw compost as well as preparation method and application thereof |
| CN113716985A (en) * | 2021-04-01 | 2021-11-30 | 北京市农林科学院 | Method for exciting organic waste to quickly become thoroughly decomposed and special exciting preparation thereof |
| CN116162574A (en) * | 2023-03-02 | 2023-05-26 | 四川农业大学 | Paenibacillus and method for solid-state fermentation of coarse fodder by using same |
| CN116162574B (en) * | 2023-03-02 | 2023-10-31 | 四川农业大学 | Paenibacillus and method for solid-state fermentation of coarse fodder by using same |
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