CN105941485A - Efficient garden herbicide - Google Patents

Efficient garden herbicide Download PDF

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Publication number
CN105941485A
CN105941485A CN201610315114.4A CN201610315114A CN105941485A CN 105941485 A CN105941485 A CN 105941485A CN 201610315114 A CN201610315114 A CN 201610315114A CN 105941485 A CN105941485 A CN 105941485A
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China
Prior art keywords
herbicide
amaranthus retroflexus
herba
candidiasis
mixing
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Pending
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CN201610315114.4A
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Chinese (zh)
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尤馨雅
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Individual
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom

Abstract

The invention belongs to the technical field of microbes and discloses an efficient garden herbicide. The efficient garden herbicide is prepared from a composite microbial agent, an adsorption carrier and soluble starch in a weight ratio being 9:4:1:2 through mixing and stirring. The herbicide adopts the microbes as an effective weeding component and has a remarkable effect on weeds such as barnyard grass, portulaca oleracea, amaranthus retroflexus, herbs of common crabgrass, copperleaf herbs, pigweed and the like.

Description

A kind of gardens highy potent herbicide
Technical field
The present invention relates to biological pesticide and preparation method thereof, be specifically related to a kind of gardens highy potent herbicide.
Background technology
Herbicide (herbicide) refers to make weeds that withered medicament occurs up hill and dale or selectively, also known as herbicide, in order to eliminate or to suppress a class material of plant growing.Sodium chlorate therein, Borax, arsenate, trichloroacetic acid have withered effect for any kind of plant, and its effect is affected by herbicide, plant and environmental condition three factor.Be divided into go out natural disposition and selective herbicide by effect, selective herbicide particularly nitrophenol, chlorophenol, carbamic derivant majority are the most effective.World Herbicide development is gradually steady, main development efficiently, low toxicity, wide spectrum, the kind of low consumption, the disposable treatment agent that environmental pollution is little is increasingly becoming main flow.
Sulfonylurea herbicide is the class herbicide that usage amount is maximum the most in the world, it is to be studied successfully by du pont company in 20 century 70s, and it is grand to develop cornfield herbicidal chlorine sulphur first in nineteen eighty-two, develop the most again efficient, wide spectrum, the low toxicity of many, high selectivity sulfonylurea herbicide.But sulfonylurea herbicide distinct issues are the long residual drug injury problems to succession crop, owing to the migration leaching of such herbicide is more weak, after its residual reaches doses, succession crop will be caused poisoning.The field of some life-time service long residual herbicides have also appeared the situation of herbicide residue amount accumulation, has a strong impact on the growth of succession crop, defines the phenomenon in " cancer field ".And there is the pesticide of Hazardous, also change soil, make alkalization of soils, pollute environment, causing serious life the most uneven, cause a lot of toy and birds extinction, the harmful substance of its residual also drastically influence the species procreation of the healthy of the mankind and the boundless universe.
Chinese patent CN1286916A discloses a kind of coal-base acid herbicide, is by Metalaxyl MZ with herbicide by weight: Metalaxyl MZ: herbicide=(0.004 5): 1 mixs homogeneously and forms.There is diseases prevention, disease-resistant, to promoting plant growth, increasing production, increase income and improve the effect of crop quality;Chinese patent CN104757000A discloses a kind of novel tertiary herbicidal composition, and it includes, the effective ingredient being made up of with herbicide C herbicide A, herbicide B, herbicide A be MCPA,MCP,methoxone sodium, herbicide B be bentazone, herbicide C be azoles oxadiazon;It is significantly improved for the prevention effect of weeds, thus realizes the more preferable auxiliary to plant growth, but, the existence of above-mentioned herbicide is not easy degraded, and environment easily causes permanent impact.
And biological pesticide is a kind of class pesticidal preparations utilizing biological living or its metabolite to prevent and treat the harmful organisms such as insect, pathogenic bacteria, weeds, nematicide, muroid, biological pesticide includes four classes such as microbial pesticide, botanical pesticide, animal pesticide and genetically modified organism pesticide, biological pesticide has the advantage that 1 compared with chemical pesticide) selectivity is strong, safer to people and animals and various beneficial organism;2) little to eco-environmental impact.Now in agricultural production, cause agroecological environment increasingly deteriorate owing to being excessively used chemical pesticide;Agricultural production efficiency is low;The severe contamination of ecological environment and the Drug resistance of plant disease strengthen;The health of the mankind is also created impact.And biological pesticide to possess the pollution to environment few;Easily decomposed by other biological or natural cause and destroy;It is difficult to the advantages such as accumulation in the environment.Biological pesticide mainly includes biological insecticides, antibacterial and ecological agricultural chemical etc..Wherein, the antibacterial antibacterial application with bacillus subtilis as main component is the most extensive.The 50's of 20th century, the reported first such as the Israel Gutter bacillus subtilis antagonism (Gutter, 1953) to citrusfruit pathogen;The U.S. has 4 bacillus subtilis biocontrol bacterial strains so far and obtains Environmental Protection Agency's commercialization or the license of limited merchandized handling application, therein QST713 bacterial strain is developed active bacteria formulation antibacterial Serenade by AgraQuest company of the U.S., and in 2000 by the registration of EPA, is used for preventing and treating the leaf diseases such as vegetable, Fructus Pruni pseudocerasi, Fructus Vitis viniferae and calabash.
Currently in order to improve the efficiency of herbicidal formulations, people generally use multiple bacterial strain to compound, compounding use is possible not only to play the advantage that chemical agent bactericidal speed is fast, prevention effect is stable, the most also has holding Biocontrol microorganism determining around plant roots, leaf and grows and secrete the features such as antibiotic.How to select suitable bacterial strain to be compounded, and reach the purpose having complementary advantages, consumption reduces, preventive effect strengthens, be to compound the problem that herbicidal formulations needs to solve.
Summary of the invention
It is an object of the invention to overcome the deficiency of prior art to provide a kind of gardens highy potent herbicide.
For realizing the object of the invention, the application adopts the following technical scheme that
A kind of gardens highy potent herbicide, it is obtained according to the weight ratio mix and blend of 9:4:1:2 by complex micro organism fungicide, absorption carrier, microorganism nutriment, soluble starch.
The preparation method of described complex micro organism fungicide is: Strepiomyces lavendulae, yellowish green wood enzyme, candidiasis, Amaranthus retroflexus biocontrol microorganisms are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 3:3:5:2, to obtain final product;
Described Strepiomyces lavendulae (Streptomyces lavendulae) is CGMCC NO.2130;
Described yellowish green wood enzyme be yellowish green wood enzyme (Trichoderma aureoviride) ACCC No.32248;
Described candidiasis be candidiasis (Candida utilis) ATCC No.22023;
Described Amaranthus retroflexus biocontrol microorganisms is Amaranthus retroflexus biocontrol microorganisms A.ama-ranthi-3.
The preparation method of described microorganism nutriment is: pulverized by rice straw, compound is obtained according to the quality of 1:1 than mixing and stirring with Testa Tritici, it is subsequently adding the 1M potassium dihydrogen phosphate with compound equal in quality, soak 12 hours, it is passed through 100 DEG C of steam heat again to steam 6 hours, being placed in 60-70 DEG C of drying again, final powder is broken into powder that particle diameter is 100 mesh and get final product;
The preparation method of described absorption carrier is: pulverized by meerschaum, then with kieselguhr according to the quality of 2:1 than mixing and stirring, be ground to the powder of 50 mesh, to obtain final product.
The application in barnyard grass, Herba Portulacae, Amaranthus retroflexus, the control of weeds such as Herba Digitariae, Herba Acalyphae, Herba chenopodii of the described herbicide.
The host speciality having the beneficial effect that microbial herbicide of hinge structure of the present invention is its advantage, is also its major defect simultaneously.Owing to gardens are complicated ecosystems, the in most cases harm of weeds in field is all to present group's harm, seldom occurs situation about the most only being worked the mischief by a kind of weeds.Thus the specialization characteristic that microbial herbicide is to weed control so that it is it is difficult to farmland weed is effectively prevented and kill off, thus with regard to restriction micro-organisms herbicide popularization and application.Some researchs show, utilizing multiple biocontrol bacterial strain mixed developing to go out complex microorganism herbicide is the effective way solving the microbial herbicide the most single defect of anti-weeds, the study show that, bacterial strain Strepiomyces lavendulae, yellowish green wood compounding between enzyme, candidiasis, Amaranthus retroflexus biocontrol microorganisms is utilized can preferably to prevent and kill off barnyard grass, Herba Portulacae, Amaranthus retroflexus, Herba Digitariae, Herba Acalyphae, Herba chenopodii etc..This result of study provides well reference for carrying out biocontrol microorganisms mixing applied research from now on.
Herbicide of the present invention does not contains the chemical herbicide such as sulfonylurea, amide-type; avoid the accumulation of herbicide residue amount and protect soil and environment; the agricultural microorganism herbicide made has strong adaptability, good stability, is difficult to the strain of the feature such as aging; and the synergism between Appropriate application microorganism; not only good to the action effect of weeds; and it is nontoxic; ecological, environmental protective; significantly reduce dosage, spraying times and drug cost; and this herbicide formulation is convenient; holding time is long, has the strongest practical value.The present invention selects suitable absorption carrier and starch can improve the bacterial strain adsorption effect to weeds, improves weeding ratio, the simultaneously agent of compatibility microbial nutrition, improves the rate of increase of microorganism so that herbicidal effect is lasting;Absorption carrier of the present invention and microorganism nutriment employ agricultural wastes and natural mineral matter, with low cost, can save entreprise cost.
Detailed description of the invention
Embodiment one
A kind of gardens highy potent herbicide, it is obtained according to the weight ratio mix and blend of 9:4:1:2 by complex micro organism fungicide, absorption carrier, microorganism nutriment, soluble starch.
The preparation method of described complex micro organism fungicide is: Strepiomyces lavendulae, yellowish green wood enzyme, candidiasis, Amaranthus retroflexus biocontrol microorganisms are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 3:3:5:2, to obtain final product;
Described Strepiomyces lavendulae (Streptomyces lavendulae) is CGMCC NO.2130(CN101481669);
Described yellowish green wood enzyme be yellowish green wood enzyme (Trichoderma aureoviride) ACCC No.32248;
Described candidiasis be candidiasis (Candida utilis) ATCC No.22023;
Described Amaranthus retroflexus biocontrol microorganisms is Amaranthus retroflexus biocontrol microorganisms A.ama-ranthi-3(for example, see the biocontrol microorganisms Alternaria amaranthi-3 prevention effect to Amaranthus retroflexus, Jiang Shujun);
The preparation method of described microorganism nutriment is: pulverized by rice straw, compound is obtained according to the quality of 1:1 than mixing and stirring with Testa Tritici, it is subsequently adding the 1M potassium dihydrogen phosphate with compound equal in quality, soak 12 hours, it is passed through 100 DEG C of steam heat again to steam 6 hours, being placed in 60-70 DEG C of drying again, final powder is broken into powder that particle diameter is 100 mesh and get final product;
The preparation method of described absorption carrier is: pulverized by meerschaum, then with kieselguhr according to the quality of 2:1 than mixing and stirring, be ground to the powder of 50 mesh, to obtain final product;
Gardens of the present invention highy potent herbicide amount of application is 15ml/m2Spray.
Embodiment two
Using greenhouse pot culture method, each weeds target is carried out plant sprinkling process, each process in triplicate, stirs before sprinkling.Process is placed in greenhouse growth, and plant reaction symptom is observed in timing, and according to 0-100% staging visual assessment comprehensive activity of weeding, evaluation criterion was shown in Table 1 in about 5,10 days after medicine.
Table 1 evaluation criterion
Activity of weeding (%) The comprehensive comment of activity of weeding (to plants stems or root suppression, deformity, albefaction, the influence degree such as rotten)
0 With comparison, inactive
10-30 Activity is low, slightly affects
40-50 Activity is low, has a significant effect growth
60-70 Active, can Developing restraint
80-90 Activity is good, serious Developing restraint
100 Plant is dead
After spraying 5 days, result of the test is as shown in table 2:
Table 2 medication 5 days
The comprehensive comment of medication Herba chenopodii Herba Portulacae Barnyard grass Amaranthus retroflexus Herba Digitariae Herba Acalyphae
85 90 85 90 90 85
After spraying 10 days, result of the test is as shown in table 3:
Table 3 medication 10 days
The comprehensive comment of medication Herba chenopodii Herba Portulacae Barnyard grass Amaranthus retroflexus Herba Digitariae Herba Acalyphae
100 100 95 95 100 95
Embodiment three
Experimental site selects greenery patches at the three of certain park, sprays and is administered, stirs, and investigate weeds radix before dispenser, 5,7,10 days difference experimental investigation ground weed densities after dispenser, and calculates preventive effect.Use absolute value survey method, in each greenery patches, randomly choose 3 points be sampled investigation, every 1, investigate every kind of weeds total strain number, carry out statistical analysis with Duncan ' s duncan's new multiple range method.
Experimental result shows, Herba chenopodii, Herba Portulacae, barnyard grass, Herba Digitariae, the density preventive effect of Herba Acalyphae is respectively 82.03%, 80.87%, 87.53%, 88.55%, 82.44% in after dispenser the 5th day;After medicine, the density preventive effect of 7 days is respectively 90.37%, 90.42%, 92.68%, 89.75%, 92.07%;After medicine, the density preventive effect of 10 days is respectively 99.37%, 100.00%, 93.85%, 97.21%, 98.54%.
Although, the present invention is described in detail the most with a general description of the specific embodiments, but on the basis of the present invention, can make some modifications or improvements it, and this will be apparent to those skilled in the art.Therefore, these amendments made without departing from theon the basis of the spirit of the present invention, improve or the expansion of scope, belong to the scope of protection of present invention.

Claims (4)

1. a gardens highy potent herbicide, it is obtained according to the weight ratio mix and blend of 9:4:1:2 by complex micro organism fungicide, absorption carrier, microorganism nutriment, soluble starch.
Herbicide the most according to claim 1, it is characterised in that
The preparation method of described complex micro organism fungicide is: Strepiomyces lavendulae, yellowish green wood enzyme, candidiasis, Amaranthus retroflexus biocontrol microorganisms are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 3:3:5:2, to obtain final product;
Described Strepiomyces lavendulae (Streptomyces lavendulae) is CGMCC NO.2130;
Described yellowish green wood enzyme be yellowish green wood enzyme (Trichoderma aureoviride) ACCC No.32248;
Described candidiasis be candidiasis (Candida utilis) ATCC No.22023;
Described Amaranthus retroflexus biocontrol microorganisms is Amaranthus retroflexus biocontrol microorganisms A.ama-ranthi-3.
Herbicide the most according to claim 1, it is characterised in that
The preparation method of described microorganism nutriment is: pulverized by rice straw, compound is obtained according to the quality of 1:1 than mixing and stirring with Testa Tritici, it is subsequently adding the 1M potassium dihydrogen phosphate with compound equal in quality, soak 12 hours, it is passed through 100 DEG C of steam heat again to steam 6 hours, being placed in 60-70 DEG C of drying again, final powder is broken into powder that particle diameter is 100 mesh and get final product;
The preparation method of described absorption carrier is: pulverized by meerschaum, then with kieselguhr according to the quality of 2:1 than mixing and stirring, be ground to the powder of 50 mesh, to obtain final product.
4. claim 1-3 is allowed to the application in barnyard grass, Herba Portulacae, Amaranthus retroflexus, the control of weeds such as Herba Digitariae, Herba Acalyphae, Herba chenopodii of the herbicide described in.
CN201610315114.4A 2016-05-14 2016-05-14 Efficient garden herbicide Pending CN105941485A (en)

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Cited By (3)

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CN106962405A (en) * 2017-03-27 2017-07-21 陕西博秦生物工程有限公司 A kind of application of close rotation streptomycete in parasitics weeds are prevented and kill off
CN108522564A (en) * 2018-05-15 2018-09-14 何治伟 A kind of preparation method of rice herbicide
CN109232075A (en) * 2018-08-23 2019-01-18 广西乐土生物科技有限公司 Special fertilizer with activity of weeding

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106962405A (en) * 2017-03-27 2017-07-21 陕西博秦生物工程有限公司 A kind of application of close rotation streptomycete in parasitics weeds are prevented and kill off
CN108522564A (en) * 2018-05-15 2018-09-14 何治伟 A kind of preparation method of rice herbicide
CN109232075A (en) * 2018-08-23 2019-01-18 广西乐土生物科技有限公司 Special fertilizer with activity of weeding

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