CN101629204A - 在缺乏酶的黑曲霉突变体中生产生物物质的方法 - Google Patents

在缺乏酶的黑曲霉突变体中生产生物物质的方法 Download PDF

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CN101629204A
CN101629204A CN200910167050A CN200910167050A CN101629204A CN 101629204 A CN101629204 A CN 101629204A CN 200910167050 A CN200910167050 A CN 200910167050A CN 200910167050 A CN200910167050 A CN 200910167050A CN 101629204 A CN101629204 A CN 101629204A
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ala
enzyme
aspergillus niger
leu
ser
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玛丽亚·康奈利
霍华德·布罗迪
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Novozymes Inc
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/40—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
CN200910167050A 2003-03-31 2004-03-31 在缺乏酶的黑曲霉突变体中生产生物物质的方法 Pending CN101629204A (zh)

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US45990203P 2003-03-31 2003-03-31
US60/459,902 2003-03-31

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CNB2004800151129A Expired - Lifetime CN100547078C (zh) 2003-03-31 2004-03-31 在缺乏酶的黑曲霉突变体中生产生物物质的方法

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US (3) US7303877B2 (https=)
EP (1) EP1613761B1 (https=)
JP (1) JP4638418B2 (https=)
CN (2) CN101629204A (https=)
AT (1) ATE441664T1 (https=)
DE (1) DE602004022929D1 (https=)
DK (1) DK1613761T3 (https=)
WO (1) WO2004090155A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114736880A (zh) * 2022-06-09 2022-07-12 中国农业科学院北京畜牧兽医研究所 酸稳定性提高葡萄糖氧化酶GoxM10的突变体D497N及其衍生突变体和应用

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101094919B (zh) * 2004-10-12 2014-09-03 帝斯曼知识产权资产管理有限公司 在生产多肽的方法中有用的真菌转录活化因子
EP1831384B1 (en) 2004-12-22 2015-08-12 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US7888489B2 (en) 2005-01-24 2011-02-15 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EP1954812B1 (en) 2005-11-29 2012-11-21 DSM IP Assets B.V. Dna binding site of a transcriptional activator useful in gene expression
WO2008000632A1 (en) 2006-06-29 2008-01-03 Dsm Ip Assets B.V. A method for achieving improved polypeptide expression
EP2511372A1 (en) 2006-11-02 2012-10-17 DSM IP Assets B.V. Improved production of secreted proteins by filamentous fungi
EA018840B1 (ru) 2007-02-15 2013-11-29 ДСМ АйПи АССЕТС Б.В. Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес
WO2009073709A1 (en) 2007-12-06 2009-06-11 Novozymes A/S Polypeptides having acetylxylan esterase activity and polynucleotides encoding same
WO2010108918A1 (en) 2009-03-24 2010-09-30 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
EP2421986A1 (en) 2009-04-22 2012-02-29 DSM IP Assets B.V. Process for the production of a recombinant polypeptide of interest
CA2758396C (en) 2009-04-24 2018-06-12 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
DK2513290T3 (en) * 2009-12-18 2016-01-25 Novozymes Inc Methods for Preparation of Polypeptides in Trichoderma Mutants with Protease Deficiency
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
CN101906393B (zh) * 2010-06-28 2012-07-25 江南大学 一种谷氨酰胺转胺酶编码基因阻断吸水链霉菌及其应用
MX2012015144A (es) 2010-06-29 2013-03-05 Dsm Ip Assets Bv Polipeptido que tiene actividad de acetil-xilan-esterasa y usos del mismo.
EP2588493A1 (en) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptide having swollenin activity and uses thereof
EP2588601A1 (en) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
DK2588603T3 (en) 2010-06-29 2017-07-03 Dsm Ip Assets Bv POLYPEPTIDE THAT HAS OR CONTRIBUTES TO CARBOHYDRATE MATERIAL DEGRADING ACTIVITIES AND APPLICATIONS THEREOF
WO2012000886A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
ES2672125T3 (es) 2010-06-29 2018-06-12 Dsm Ip Assets B.V. Polipéptido que tiene actividad beta-glucosidasa y usos del mismo
US9096871B2 (en) 2010-10-06 2015-08-04 Bp Corporation North America Inc. Variant CBH I polypeptides with reduced product inhibition
US20120276247A1 (en) 2011-04-29 2012-11-01 Uwe Bornscheuer Lipase Variants
JP5846476B2 (ja) * 2011-07-08 2016-01-20 独立行政法人酒類総合研究所 微生物のコウジ酸生産性を向上させる方法
CN102296032B (zh) * 2011-08-31 2013-11-06 保龄宝生物股份有限公司 转葡糖苷酶及其制备和固定化方法
JP5975236B2 (ja) * 2011-11-02 2016-08-23 ユニバーシティ プトゥラ マレーシア 生体高分子の製造方法
RU2645252C2 (ru) 2012-01-05 2018-02-19 Глюкос Финланд Ой Клетка нитчатых грибов с дефицитом протеаз и способы ее применения
EP2852610B1 (en) 2012-05-23 2018-07-11 Glykos Finland Oy Production of fucosylated glycoproteins
CN104603269A (zh) 2012-08-17 2015-05-06 诺维信公司 用于在丝状真菌菌株中共沉默基因表达的方法及其应用
SG11201600115SA (en) 2013-07-10 2016-02-26 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
US10619144B2 (en) 2013-08-23 2020-04-14 Novozymes A/S Regulated PepC expression
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
EP3445776B1 (en) * 2016-04-19 2021-02-17 Novozymes A/S Rlma-inactivated filamentous fungal host cell
US10986850B2 (en) 2016-12-21 2021-04-27 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
CN108504615B (zh) * 2018-03-30 2020-12-29 江南大学 一种产酸性蛋白酶的重组菌及其应用
CN113301812A (zh) 2018-10-17 2021-08-24 完美日股份有限公司 用于食物产品的重组组分和组合物
US20220251581A1 (en) * 2019-07-26 2022-08-11 Novozymes A/S Modified filamentous fungal host cell
CN113265417B (zh) * 2020-02-14 2025-01-21 中国科学院天津工业生物技术研究所 有机酸产量提高的菌株及其构建方法和应用
EP4228424A1 (en) 2020-10-15 2023-08-23 DSM IP Assets B.V. Methods of modulating gastrointestinal metabolites
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023242273A1 (en) * 2022-06-14 2023-12-21 Newmilkbuzz B.V. Expression of milk caseins in filamentous fungi
WO2025230415A1 (en) 2024-04-30 2025-11-06 Ginkgo Bioworks Netherlands B.V. Host cells expressing optb protein variants and uses thereof
CN119265158B (zh) * 2024-09-27 2025-11-04 湖北大学 一种脂肪酶a突变体及其制备方法和应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5536661A (en) 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5252726A (en) 1987-09-04 1993-10-12 Novo Nordisk A/S Promoters for use in aspergillus
DK0531372T4 (da) 1990-05-09 2004-08-09 Novozymes As Cellulasepræparat omfattende et endoglucanaseenzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
FI964808A0 (fi) 1994-06-03 1996-12-02 Novo Nordisk Biotech Inc Puhdistetut Myceliophthora lakkaasit ja niitä koodaavat nukleiinihapot
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
US5646025A (en) 1995-05-05 1997-07-08 Novo Nordisk A/S Scytalidium catalase gene
KR20000069395A (ko) * 1996-12-12 2000-11-25 프로헤 레오폴드 트리파레독신, 발현 플라스미드, 제조방법, 용도, 시험 시스템및 의약 제제
JP5303084B2 (ja) * 1997-04-11 2013-10-02 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 工業的組み換え生物を溝築するための手段としての遺伝子変換
US6432371B1 (en) * 1998-09-18 2002-08-13 Jack Oliver, Jr. Child proof chlorine dispenser with a three dimensional ornament
US6806062B1 (en) * 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
DK1117798T3 (da) 1998-10-05 2007-04-10 Novozymes As Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
EP1157100B1 (en) 1999-02-22 2006-01-11 Novozymes A/S Oxaloacetate hydrolase deficient fungal host cells
CN100510096C (zh) 1999-03-22 2009-07-08 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
DE60142226D1 (de) 2000-03-14 2010-07-08 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
KR20020026456A (ko) * 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114736880A (zh) * 2022-06-09 2022-07-12 中国农业科学院北京畜牧兽医研究所 酸稳定性提高葡萄糖氧化酶GoxM10的突变体D497N及其衍生突变体和应用

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ATE441664T1 (de) 2009-09-15
US20040191864A1 (en) 2004-09-30
DK1613761T3 (da) 2009-12-14
US7303877B2 (en) 2007-12-04
WO2004090155A2 (en) 2004-10-21
EP1613761A2 (en) 2006-01-11
CN100547078C (zh) 2009-10-07
CN1798848A (zh) 2006-07-05
US7771971B2 (en) 2010-08-10
WO2004090155A3 (en) 2005-01-06
DE602004022929D1 (de) 2009-10-15
EP1613761A4 (en) 2006-08-09
JP4638418B2 (ja) 2011-02-23
JP2006521826A (ja) 2006-09-28
US8445283B2 (en) 2013-05-21
US20100323448A1 (en) 2010-12-23
EP1613761B1 (en) 2009-09-02
US20080044858A1 (en) 2008-02-21

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