CN101611139B - 利用微载体的产后来源的细胞的体外扩增 - Google Patents

利用微载体的产后来源的细胞的体外扩增 Download PDF

Info

Publication number
CN101611139B
CN101611139B CN2007800494226A CN200780049422A CN101611139B CN 101611139 B CN101611139 B CN 101611139B CN 2007800494226 A CN2007800494226 A CN 2007800494226A CN 200780049422 A CN200780049422 A CN 200780049422A CN 101611139 B CN101611139 B CN 101611139B
Authority
CN
China
Prior art keywords
cells
microcarriers
cell
microcarrier
tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2007800494226A
Other languages
English (en)
Chinese (zh)
Other versions
CN101611139A (zh
Inventor
A·M·哈蒙
L·S·K·洪
A·J·金
A·戈西夫斯卡
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johnson and Johnson Medical SAS
Original Assignee
Ethicon SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ethicon SAS filed Critical Ethicon SAS
Publication of CN101611139A publication Critical patent/CN101611139A/zh
Application granted granted Critical
Publication of CN101611139B publication Critical patent/CN101611139B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN2007800494226A 2006-11-13 2007-11-13 利用微载体的产后来源的细胞的体外扩增 Expired - Fee Related CN101611139B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US86555806P 2006-11-13 2006-11-13
US60/865,558 2006-11-13
PCT/US2007/023846 WO2008060541A2 (en) 2006-11-13 2007-11-13 In vitro expansion of postpartum-derived cells using microcarriers

Publications (2)

Publication Number Publication Date
CN101611139A CN101611139A (zh) 2009-12-23
CN101611139B true CN101611139B (zh) 2012-07-04

Family

ID=39402235

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2007800494226A Expired - Fee Related CN101611139B (zh) 2006-11-13 2007-11-13 利用微载体的产后来源的细胞的体外扩增

Country Status (9)

Country Link
US (1) US9102915B2 (https=)
EP (1) EP2089511B1 (https=)
JP (2) JP5670053B2 (https=)
CN (1) CN101611139B (https=)
AU (1) AU2007319820B2 (https=)
CA (1) CA2669589C (https=)
ES (1) ES2524443T3 (https=)
PL (1) PL2089511T3 (https=)
WO (1) WO2008060541A2 (https=)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003008535A2 (en) * 2001-07-20 2003-01-30 Technion Research And Development Foundation Ltd. Methods of generating human cardiac cells and tissues and uses thereof
US9592258B2 (en) 2003-06-27 2017-03-14 DePuy Synthes Products, Inc. Treatment of neurological injury by administration of human umbilical cord tissue-derived cells
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
US9572840B2 (en) 2003-06-27 2017-02-21 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
WO2005001080A2 (en) 2003-06-27 2005-01-06 Ethicon, Incorporated Postpartum-derived cells for use in treatment of disease of the heart and circulatory system
WO2006071794A2 (en) * 2004-12-23 2006-07-06 Ethicon Incorporated Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US8790637B2 (en) 2003-06-27 2014-07-29 DePuy Synthes Products, LLC Repair and regeneration of ocular tissue using postpartum-derived cells
US8491883B2 (en) 2003-06-27 2013-07-23 Advanced Technologies And Regenerative Medicine, Llc Treatment of amyotrophic lateral sclerosis using umbilical derived cells
US8518390B2 (en) 2003-06-27 2013-08-27 Advanced Technologies And Regenerative Medicine, Llc Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells
WO2006071778A2 (en) 2004-12-23 2006-07-06 Ethicon Incorporated Treatment of parkinson's disease and related disorders using postpartum derived cells
DK1957633T3 (en) 2005-10-13 2014-03-17 Anthrogenesis Corp Immunomodulation USING PLACE SPEECH STEM CELLS
WO2007070870A1 (en) 2005-12-16 2007-06-21 Ethicon, Inc. Compositions and methods for inhibiting adverse immune response in histocompatibility-mismatched transplantation
JP5179376B2 (ja) * 2005-12-19 2013-04-10 エシコン・インコーポレイテッド ローラーボトルでの分娩後取り出し細胞の体外増殖
US9125906B2 (en) 2005-12-28 2015-09-08 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using umbilical cord tissue-derived cells
PT2471904T (pt) 2005-12-29 2019-02-25 Celularity Inc População de células estaminais placentárias
WO2008045498A1 (en) 2006-10-12 2008-04-17 Ethicon, Inc. Kidney-derived cells and methods of use in tissue repair and regeneration
PL2089511T3 (pl) 2006-11-13 2015-02-27 Depuy Synthes Products Llc Rozrost in vitro komórek pochodzących z tkanki pępowinowej wykorzystujących mikronośniki
EP3763376A1 (en) 2007-02-12 2021-01-13 Celularity, Inc. Treatment of inflammatory diseases using placental stem cells
US8399253B2 (en) * 2007-04-28 2013-03-19 Hyunjin Yang Proliferation culture methods using micro-scaffolds for regulations of cell-to-cell signals
NZ599825A (en) 2007-09-28 2014-10-31 Anthrogenesis Corp Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells
PT103843B (pt) * 2007-10-04 2008-08-12 Medinfar Produtos Farmaceutico Método de isolamento de células precursoras a partir do cordão umbilical humano
JP5323845B2 (ja) 2007-10-05 2013-10-23 エシコン・インコーポレイテッド ヒト臍帯組織由来細胞を用いた腎組織の修復および再建
US8236538B2 (en) * 2007-12-20 2012-08-07 Advanced Technologies And Regenerative Medicine, Llc Methods for sterilizing materials containing biologically active agents
US20090233334A1 (en) * 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
US9458431B2 (en) 2008-03-17 2016-10-04 Agency For Science, Technology And Research Microcarriers for stem cell culture
JP2011514169A (ja) 2008-03-17 2011-05-06 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 幹細胞培養のためのマイクロキャリア
US8691569B2 (en) * 2008-03-17 2014-04-08 Agency For Science, Technology And Research Microcarriers for stem cell culture
US8828720B2 (en) * 2008-03-17 2014-09-09 Agency For Science, Technology And Research Microcarriers for stem cell culture
US20120219531A1 (en) * 2008-03-17 2012-08-30 Agency For Science, Technology And Research Microcarriers for Stem Cell Culture
US20120028352A1 (en) * 2008-03-17 2012-02-02 Agency For Science, Technology And Research Microcarriers for Stem Cell Culture
KR20240052847A (ko) 2008-08-20 2024-04-23 셀룰래리티 인코포레이티드 개선된 세포 조성물 및 그의 제조 방법
WO2010021715A1 (en) 2008-08-20 2010-02-25 Anthrogenesis Corporation Treatment of stroke using isolated placental cells
CA2734446C (en) 2008-08-22 2017-06-20 Anthrogenesis Corporation Methods and compositions for treatment of bone defects with placental cell populations
AU2009316541B2 (en) 2008-11-19 2015-08-06 Celularity Inc. Amnion derived adherent cells
US20100151575A1 (en) * 2008-12-15 2010-06-17 Colter David C Method of Making Conditioned Media from Kidney Derived Cells
CN102387807A (zh) 2008-12-19 2012-03-21 先进科技及再生医学有限责任公司 肺部疾病和病症的治疗
US10179900B2 (en) 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
SG174551A1 (en) 2009-03-26 2011-10-28 Ethicon Inc Human umbilical cord tissue cells as therapy for alzheimer' s disease
US20100247495A1 (en) * 2009-03-30 2010-09-30 Tom Ichim Treatment of Muscular Dystrophy
US20120189702A1 (en) * 2009-08-06 2012-07-26 Albert Einstein College Of Medicine Of Yeshiva University Cell therapy for treatment of liver failure
US8278101B2 (en) * 2009-12-07 2012-10-02 Synthecon, Inc. Stem cell bioprocessing and cell expansion
DK3284818T3 (da) 2010-01-26 2022-06-20 Celularity Inc Behandling af knoglerelateret kræft ved hjælp af placenta stamceller
LT2556145T (lt) 2010-04-07 2016-11-10 Anthrogenesis Corporation Angiogenezė naudojant placentos kamienines ląsteles
MX2012011543A (es) 2010-04-08 2013-05-06 Anthrogenesis Corp Tratamiento de sarcoidosis empleando celulas madre placentarias.
EP2575913B1 (en) 2010-05-25 2018-07-25 Cook Biotech Incorporated Methods, substrates, and systems useful for cell seeding of medical grafts
CN102146356A (zh) * 2010-06-24 2011-08-10 辽宁成大生物股份有限公司 一种再生微载体的方法
ES2666746T3 (es) 2010-07-13 2018-05-07 Anthrogenesis Corporation Métodos para generar linfocitos citolíticos naturales
US10240121B2 (en) 2010-08-16 2019-03-26 Kiyatec Inc. Bioreactor system
US8969315B2 (en) 2010-12-31 2015-03-03 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
JP2012165719A (ja) * 2011-02-16 2012-09-06 National Institute Of Advanced Industrial Science & Technology 擬微小重力環境下での三次元癌組織モデルの構築方法
JP6104896B2 (ja) 2011-06-01 2017-03-29 アントフロゲネシス コーポレーション 胎盤幹細胞を使用する疼痛の治療
US20140170748A1 (en) * 2012-12-14 2014-06-19 DePuy Synthes Products, LLC Nutrient Enriched Media for hUTC Growth
CN115137753A (zh) 2013-02-05 2022-10-04 细胞结构公司 来自胎盘的自然杀伤细胞
US10895524B2 (en) * 2013-02-19 2021-01-19 Abbott Point Of Care, Inc. Biologic fluid sample analysis cartridge with non-reflective beads
JP2016526387A (ja) * 2013-06-24 2016-09-05 アントフロゲネシス コーポレーション 細胞培養のための細胞外マトリクス組成物ビーズ
JP6913094B2 (ja) * 2015-12-22 2021-08-04 コーニング インコーポレイテッド 細胞分離器具およびそれを使用する方法
KR102211270B1 (ko) 2016-01-14 2021-02-04 디퍼이 신테스 프로덕츠, 인코포레이티드 Hutc의 동결보존을 위한 조성물 및 방법
CN109312284B (zh) 2016-05-31 2021-10-22 康宁股份有限公司 用于培养细胞的减少叶轮摆动的容器和旋转烧瓶
EP3528630A4 (en) * 2016-10-21 2020-05-13 Georgia Tech Research Corporation METHODS AND SYSTEMS FOR T-CELL EXPANSION
WO2018186185A1 (ja) 2017-04-06 2018-10-11 日機装株式会社 細胞の培養方法、細胞支持複合体の製造方法、培養細胞及び細胞支持複合体
WO2019181920A1 (ja) * 2018-03-22 2019-09-26 日機装株式会社 細胞の培養方法、細胞支持複合体の製造方法、培養細胞および細胞支持複合体
KR20210134323A (ko) 2019-01-31 2021-11-09 베이징 사이토니체 바이오테크놀로지 씨오., 엘티디. 줄기 세포의 대규모 제조를 위한 3차원 배양 방법
CN111424011A (zh) * 2020-04-17 2020-07-17 深圳市旷逸生物科技有限公司 一种能够维持脐带间充质干细胞细胞形态的三维培养方法
US20230034857A1 (en) * 2020-09-15 2023-02-02 Lg Chem, Ltd. Microcarrier for cell culture and method for preparing the same
JPWO2024253069A1 (https=) * 2023-06-06 2024-12-12

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1220832B (de) * 1964-09-22 1966-07-14 Siemens Ag Ziehduese zum Ziehen von Halbleiterkristallen aus einer Schmelze
US3665061A (en) 1969-07-16 1972-05-23 United States Banknote Corp Process for producing collagen sponges
US4216144A (en) 1977-10-20 1980-08-05 Ashmead H H Soluble iron proteinates
JPS6040439B2 (ja) 1978-03-29 1985-09-11 大正製薬株式会社 ヒドロコルチゾン誘導体
US4487865A (en) 1983-12-15 1984-12-11 Biomatrix, Inc. Polymeric articles modified with hyaluronate
US5266476A (en) * 1985-06-18 1993-11-30 Yeda Research & Development Co., Ltd. Fibrous matrix for in vitro cell cultivation
US4963489A (en) * 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5266480A (en) * 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
US4925677A (en) 1988-08-31 1990-05-15 Theratech, Inc. Biodegradable hydrogel matrices for the controlled release of pharmacologically active agents
US5284766A (en) * 1989-02-10 1994-02-08 Kao Corporation Bed material for cell culture
NL9100038A (nl) 1991-01-11 1992-08-03 Stamicarbon Enzym-gekatalyseerde bereiding van optisch aktieve carbonzuren.
DE4406073A1 (de) 1994-02-24 1995-08-31 Univ Ludwigs Albert Verfahren zur Herstellung von humanen, klonogenen Fibroblasten, Verfahren zur Gentransfizierung von Fibroblasten und so erhaltene Fibroblasten
US5919702A (en) 1996-10-23 1999-07-06 Advanced Tissue Science, Inc. Production of cartilage tissue using cells isolated from Wharton's jelly
JP2002513383A (ja) 1997-05-21 2002-05-08 スローン−ケタリング・インスティテュート・フォー・キャンサー・リサーチ 患者の脳組織でのアスコルビン酸の濃度を増加させる方法
US7560280B2 (en) * 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
EP2316919B1 (en) * 2001-02-14 2015-10-07 Anthrogenesis Corporation Post-partum mammalian placenta, its use and placental stem cells therefrom
EP1384786B1 (en) 2001-04-02 2010-01-27 Glycomedics, Inc. Process for producing oligosaccharide chains
AU2003217350A1 (en) * 2002-02-08 2003-09-02 University Of Chile Proliferated cell lines and uses thereof
ES2287447T3 (es) 2002-02-19 2007-12-16 Medipost, Co., Ltd. Metodos de aislamiento y expansion del cultivo de celulas troncales/madre mesenquimatosas a partir de sangre del cordon umbilical, y metodo de diferenciacion de celulas troncales/madre mesenquimatosas derivadas de sangre del cordon umbilical en diversos tejidos mesenquimatosos.
JP2003259862A (ja) * 2002-03-12 2003-09-16 Fuji Photo Film Co Ltd 細胞培養担体
AU2003265094A1 (en) 2002-08-26 2004-03-11 Neuronova Ab Method for culturing stem cells
GB0222846D0 (en) * 2002-10-03 2002-11-06 Choo Yen Cell culture
US7875272B2 (en) * 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
US8790637B2 (en) * 2003-06-27 2014-07-29 DePuy Synthes Products, LLC Repair and regeneration of ocular tissue using postpartum-derived cells
WO2005001080A2 (en) 2003-06-27 2005-01-06 Ethicon, Incorporated Postpartum-derived cells for use in treatment of disease of the heart and circulatory system
WO2006071794A2 (en) * 2004-12-23 2006-07-06 Ethicon Incorporated Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US9572840B2 (en) 2003-06-27 2017-02-21 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
CA2532754A1 (en) 2003-07-17 2005-02-03 Global Cell Solutions, Llc Automated cell culture system and process
JP4575654B2 (ja) * 2003-09-05 2010-11-04 エスエス製薬株式会社 溶解性と流動性を改善した医薬組成物
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
AU2005302516A1 (en) 2004-10-29 2006-05-11 Centocor, Inc. Chemically defined media compositions
US20060166361A1 (en) 2004-12-21 2006-07-27 Agnieszka Seyda Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same
WO2006071778A2 (en) * 2004-12-23 2006-07-06 Ethicon Incorporated Treatment of parkinson's disease and related disorders using postpartum derived cells
WO2006088867A2 (en) * 2005-02-15 2006-08-24 Medistem Laboratories, Incorporated Method for expansion of stem cells
CN103356706B (zh) 2005-03-31 2016-01-20 斯丹姆涅恩有限公司 促进无疤愈合或改善皮肤外观的美容制剂
WO2007070870A1 (en) * 2005-12-16 2007-06-21 Ethicon, Inc. Compositions and methods for inhibiting adverse immune response in histocompatibility-mismatched transplantation
JP5179376B2 (ja) * 2005-12-19 2013-04-10 エシコン・インコーポレイテッド ローラーボトルでの分娩後取り出し細胞の体外増殖
EP2366775B1 (en) * 2006-03-23 2015-04-22 Pluristem Ltd. Methods for cell expansion and uses of cells and conditioned media produced thereby for therapy
CA2893679C (en) 2006-06-26 2018-10-30 Lifescan, Inc. Conditioned medium for pluripotent stem cell culture
PL2089511T3 (pl) 2006-11-13 2015-02-27 Depuy Synthes Products Llc Rozrost in vitro komórek pochodzących z tkanki pępowinowej wykorzystujących mikronośniki
JP6095893B2 (ja) 2008-12-19 2017-03-15 デピュイ・シンセス・プロダクツ・インコーポレイテッド 傷害後の神経組織の再生および修復
US10179900B2 (en) 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
BRPI0923177A2 (pt) 2008-12-19 2020-08-25 Advanced Technologies And Regenerative Medicine, Llc usos de composições para tratar dor neuropática e espacidade, as referidas composições, e kit
SG174551A1 (en) * 2009-03-26 2011-10-28 Ethicon Inc Human umbilical cord tissue cells as therapy for alzheimer' s disease

Also Published As

Publication number Publication date
CA2669589C (en) 2016-01-12
WO2008060541A2 (en) 2008-05-22
US9102915B2 (en) 2015-08-11
CN101611139A (zh) 2009-12-23
CA2669589A1 (en) 2008-05-22
AU2007319820B2 (en) 2013-09-05
EP2089511B1 (en) 2014-09-17
ES2524443T3 (es) 2014-12-09
AU2007319820A1 (en) 2008-05-22
JP2014236755A (ja) 2014-12-18
US20080166328A1 (en) 2008-07-10
WO2008060541A3 (en) 2008-08-28
JP5670053B2 (ja) 2015-02-18
JP2010508851A (ja) 2010-03-25
PL2089511T3 (pl) 2015-02-27
EP2089511A2 (en) 2009-08-19

Similar Documents

Publication Publication Date Title
CN101611139B (zh) 利用微载体的产后来源的细胞的体外扩增
JP2010508851A5 (https=)
CN105026551B (zh) 用于hUTC生长的营养富集培养基
JP7588591B2 (ja) 幹細胞の大規模調製のための三次元培養方法
JP2012509085A (ja) マイクロキャリア上での多能性幹細胞の培養
WO2021009778A2 (en) Methods for culturing mesenchymal stem cells, products thereof, and applications thereof
US20230220349A1 (en) 3d culture of mesenchymal lineage precursor or stem cells
WO2024251234A1 (zh) 间充质干细胞无血清培养基及其应用
CN110951686A (zh) 一种造血干细胞体外扩增培养体系和方法
EP4638704A1 (en) Cell culturing of adherent cells

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120704

CF01 Termination of patent right due to non-payment of annual fee