CN101605891A - PrP基因的遗传学消融使用靶定启动子陷阱法生产作为治疗剂的无血清重组蛋白的细胞 - Google Patents

PrP基因的遗传学消融使用靶定启动子陷阱法生产作为治疗剂的无血清重组蛋白的细胞 Download PDF

Info

Publication number
CN101605891A
CN101605891A CNA2007800466531A CN200780046653A CN101605891A CN 101605891 A CN101605891 A CN 101605891A CN A2007800466531 A CNA2007800466531 A CN A2007800466531A CN 200780046653 A CN200780046653 A CN 200780046653A CN 101605891 A CN101605891 A CN 101605891A
Authority
CN
China
Prior art keywords
cell
prp
clone
target
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2007800466531A
Other languages
English (en)
Chinese (zh)
Inventor
卡罗拉·施罗德
伊丽莎白·卡萨德蒙特
基姆·比约恩斯特普
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Octagene GmbH
Original Assignee
Octagene GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Octagene GmbH filed Critical Octagene GmbH
Publication of CN101605891A publication Critical patent/CN101605891A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNA2007800466531A 2006-11-20 2007-11-20 PrP基因的遗传学消融使用靶定启动子陷阱法生产作为治疗剂的无血清重组蛋白的细胞 Pending CN101605891A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06124427 2006-11-20
EP06124427.3 2006-11-20

Publications (1)

Publication Number Publication Date
CN101605891A true CN101605891A (zh) 2009-12-16

Family

ID=37685785

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2007800466531A Pending CN101605891A (zh) 2006-11-20 2007-11-20 PrP基因的遗传学消融使用靶定启动子陷阱法生产作为治疗剂的无血清重组蛋白的细胞

Country Status (12)

Country Link
EP (1) EP2084269A2 (no)
KR (1) KR20090106474A (no)
CN (1) CN101605891A (no)
AU (1) AU2007324530A1 (no)
BR (1) BRPI0718989A2 (no)
CA (1) CA2670003A1 (no)
IL (1) IL198770A0 (no)
MX (1) MX2009005277A (no)
NO (1) NO20091875L (no)
RU (1) RU2009123458A (no)
WO (1) WO2008061995A2 (no)
ZA (1) ZA200903429B (no)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2728900T3 (es) * 2010-05-12 2019-10-29 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas
CN102277379B (zh) * 2011-08-18 2013-07-24 中国科学院遗传与发育生物学研究所 表达凝血因子viii的表达载体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
ATE288484T1 (de) * 1991-11-14 2005-02-15 Charles Weissmann Transgene nicht-menschliche tiere ohne prionprotein
NZ512309A (en) * 1998-12-22 2003-05-30 Univ California Prion-free therapeutic formulations produced from somatic cells with an artificially altered prion protein (PrP) gene
AU2001247773A1 (en) * 2000-03-24 2001-10-08 University Of Massachusetts, As Represented By Its Amherst Campus Prion-free transgenic ungulates
AU2002242854A1 (en) * 2001-03-21 2002-10-03 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals

Also Published As

Publication number Publication date
WO2008061995A2 (en) 2008-05-29
CA2670003A1 (en) 2008-05-29
RU2009123458A (ru) 2010-12-27
EP2084269A2 (en) 2009-08-05
KR20090106474A (ko) 2009-10-09
IL198770A0 (en) 2011-08-01
WO2008061995A3 (en) 2008-07-10
AU2007324530A1 (en) 2008-05-29
MX2009005277A (es) 2009-05-28
NO20091875L (no) 2009-07-28
BRPI0718989A2 (pt) 2014-02-11
ZA200903429B (en) 2010-04-28

Similar Documents

Publication Publication Date Title
KR101320489B1 (ko) 인간 세포주에서 재조합 인간 단백질의 무혈청의 안정한형질감염 및 생산
KR20210056329A (ko) 신규 cas12b 효소 및 시스템
KR101828828B1 (ko) 항-히알루로난 제제 치료를 위한 동반 진단 및 이의 사용 방법
KR20230091894A (ko) 부위 특이적 표적화 요소를 통한 프로그램 가능한 첨가(paste)를 사용하는 부위 특이적 유전 공학을 위한 시스템, 방법, 및 조성물
KR20190065251A (ko) 모듈러 AAV 전달 시스템을 통한 CRISPR-Cas 게놈 가공
KR20090122465A (ko) 염증성 질환의 치료
KR102652494B1 (ko) 전장 t-세포 수용체 오픈 리딩 프레임의 신속한 조립 및 다양화를 위한 2-성분 벡터 라이브러리 시스템
KR101921698B1 (ko) 재조합에 의해 제조된 인간 인자 ⅷ 및 ⅸ
KR102614328B1 (ko) T-세포 수용체 합성 및 tcr-제시 세포에 대한 안정적인 게놈 통합을 위한 2-부분 디바이스
CN110684804B (zh) 递送外源rnp的慢病毒载体及其制备方法
CN112168958B (zh) 基于慢病毒外壳修饰和mRNA递送的SARS-CoV-2疫苗及其制备方法
KR20160016856A (ko) 말라리아 백신
KR20220047623A (ko) 세포 유형 운명 특정화의 조절인자를 확인하기 위한 조성물 및 방법
KR20160002880A (ko) 엔도솜 포집을 극복하기 위해 설계된 인공 전사 인자
JP2024037917A (ja) 組換えｔ細胞受容体遺伝子を用いて細胞ベースの治療薬を製造するための技法
CN111094569A (zh) 光控性病毒蛋白质、其基因及包含该基因的病毒载体
CN101657097A (zh) 以炎症为特征的疾病的治疗
KR20160003691A (ko) Opa1 단상부족에 의해 원인이 되는 질병 치료를 위한 인공전사인자
CN113474453B (zh) 用于治疗iv a型粘多糖的腺相关病毒载体
CN109762846B (zh) 利用碱基编辑修复与克拉伯病相关的galcc1586t突变的试剂和方法
CN110042124A (zh) 基因组碱基编辑增加人红细胞中胎儿血红蛋白水平的试剂盒及应用
CN101605891A (zh) PrP基因的遗传学消融使用靶定启动子陷阱法生产作为治疗剂的无血清重组蛋白的细胞
US11814412B2 (en) Artificial proteins and compositions and methods thereof
CN114058607B (zh) 一种用于c到u碱基编辑的融合蛋白及其制备方法和应用
CN110819656B (zh) 一种基于同步光源的x-射线多色遗传标记探针及其制备方法以及应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20091216