CN101600793B - 用于获得抗原特异性b细胞的克隆群体的培养方法 - Google Patents

用于获得抗原特异性b细胞的克隆群体的培养方法 Download PDF

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Publication number
CN101600793B
CN101600793B CN200780027028.2A CN200780027028A CN101600793B CN 101600793 B CN101600793 B CN 101600793B CN 200780027028 A CN200780027028 A CN 200780027028A CN 101600793 B CN101600793 B CN 101600793B
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antigen
cell
antibody
cells
specific
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CN101600793A (zh
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J·莱瑟姆
L·加西亚
A·E·C·詹森
E·奥加拉
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H Lundbeck AS
Lundbeck Seattle Biopharmaceuticals Inc
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Alder Biopharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/248—IL-6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
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  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200780027028.2A 2006-05-19 2007-05-21 用于获得抗原特异性b细胞的克隆群体的培养方法 Expired - Fee Related CN101600793B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US80141206P 2006-05-19 2006-05-19
US60/801,412 2006-05-19
PCT/US2007/011980 WO2008045140A1 (en) 2006-05-19 2007-05-21 Culture method for obtaining a clonal population of antigen-specific b cells

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CN101600793A CN101600793A (zh) 2009-12-09
CN101600793B true CN101600793B (zh) 2013-06-26

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US (3) US20070269868A1 (cg-RX-API-DMAC10.html)
EP (1) EP2021463B1 (cg-RX-API-DMAC10.html)
JP (1) JP5607357B2 (cg-RX-API-DMAC10.html)
CN (1) CN101600793B (cg-RX-API-DMAC10.html)
AU (1) AU2007307324B2 (cg-RX-API-DMAC10.html)
CA (1) CA2652392C (cg-RX-API-DMAC10.html)
DK (1) DK2021463T3 (cg-RX-API-DMAC10.html)
IL (1) IL195285A (cg-RX-API-DMAC10.html)
MX (1) MX2008014692A (cg-RX-API-DMAC10.html)
NO (1) NO20084835L (cg-RX-API-DMAC10.html)
NZ (1) NZ572807A (cg-RX-API-DMAC10.html)
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CN101600793A (zh) 2009-12-09
DK2021463T3 (en) 2017-01-16
JP5607357B2 (ja) 2014-10-15
CA2652392A1 (en) 2008-04-17
AU2007307324A1 (en) 2008-04-17
NZ572807A (en) 2011-10-28
JP2009537176A (ja) 2009-10-29
US20130316353A1 (en) 2013-11-28
CA2652392C (en) 2017-10-10
US20070269868A1 (en) 2007-11-22
NO20084835L (no) 2009-02-16
IL195285A0 (en) 2011-08-01
WO2008045140A1 (en) 2008-04-17
EP2021463B1 (en) 2016-11-23
IL195285A (en) 2012-07-31
US20120141982A1 (en) 2012-06-07
AU2007307324B2 (en) 2013-08-15
MX2008014692A (es) 2009-08-18
EP2021463A1 (en) 2009-02-11
EP2021463A4 (en) 2009-09-02

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