CN101591617A - 一种二十二碳六烯酸生产菌株及其诱变筛选方法和其应用 - Google Patents
一种二十二碳六烯酸生产菌株及其诱变筛选方法和其应用 Download PDFInfo
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- CN101591617A CN101591617A CNA2009100334938A CN200910033493A CN101591617A CN 101591617 A CN101591617 A CN 101591617A CN A2009100334938 A CNA2009100334938 A CN A2009100334938A CN 200910033493 A CN200910033493 A CN 200910033493A CN 101591617 A CN101591617 A CN 101591617A
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- Prior art keywords
- mutagenesis
- strain
- screening method
- docosahexaenoic acid
- producing strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 title claims abstract description 159
- 235000020669 docosahexaenoic acid Nutrition 0.000 title claims abstract description 75
- 229940090949 docosahexaenoic acid Drugs 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 40
- 238000012216 screening Methods 0.000 title claims abstract description 34
- 230000035772 mutation Effects 0.000 title abstract 2
- 231100000350 mutagenesis Toxicity 0.000 claims abstract description 45
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- 230000004151 fermentation Effects 0.000 claims abstract description 21
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- 241000894006 Bacteria Species 0.000 claims description 23
- 238000011282 treatment Methods 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- DVSZKTAMJJTWFG-UHFFFAOYSA-N docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCCC=CC=CC=CC=CC=CC=CC(O)=O DVSZKTAMJJTWFG-UHFFFAOYSA-N 0.000 claims description 13
- 239000003112 inhibitor Substances 0.000 claims description 13
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- 238000011218 seed culture Methods 0.000 claims description 12
- 239000002028 Biomass Substances 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 230000012010 growth Effects 0.000 claims description 8
- 239000001103 potassium chloride Substances 0.000 claims description 8
- 235000011164 potassium chloride Nutrition 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 241001192924 Parna Species 0.000 claims description 7
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 239000002962 chemical mutagen Substances 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
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- 150000004665 fatty acids Chemical class 0.000 claims description 5
- JDNTWHVOXJZDSN-UHFFFAOYSA-N iodoacetic acid Chemical group OC(=O)CI JDNTWHVOXJZDSN-UHFFFAOYSA-N 0.000 claims description 5
- 230000010355 oscillation Effects 0.000 claims description 5
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 4
- HCPOCMMGKBZWSJ-UHFFFAOYSA-N ethyl 3-hydrazinyl-3-oxopropanoate Chemical group CCOC(=O)CC(=O)NN HCPOCMMGKBZWSJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000012262 fermentative production Methods 0.000 claims description 4
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- AGDSCTQQXMDDCV-UHFFFAOYSA-M sodium;2-iodoacetate Chemical compound [Na+].[O-]C(=O)CI AGDSCTQQXMDDCV-UHFFFAOYSA-M 0.000 claims description 4
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- PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 claims description 3
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- 159000000007 calcium salts Chemical class 0.000 claims description 2
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- 229910017053 inorganic salt Inorganic materials 0.000 claims description 2
- 159000000003 magnesium salts Chemical class 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 claims description 2
- 150000003016 phosphoric acids Chemical class 0.000 claims description 2
- 230000035040 seed growth Effects 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 159000000000 sodium salts Chemical class 0.000 claims description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 abstract description 6
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- 238000005516 engineering process Methods 0.000 abstract description 5
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 7
- 150000002632 lipids Chemical class 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
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- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 4
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 229920001817 Agar Polymers 0.000 description 2
- 241000235575 Mortierella Species 0.000 description 2
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- 206010047400 Vibrio infections Diseases 0.000 description 2
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 2
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 2
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- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 2
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
生物量 | 油脂含量 | DHA占总油脂 | DHA产量 | 发酵时间 | DHA产率 | |
原始菌株 | 21g/L | 14g/L | 28% | 5.32g/L | 96h | 0.063g/L·h |
菌株HX-308 | 37g/L | 20.4g/L | 40% | 8.16g/L | 75h | 0.1088g/L·h |
生物量 | 油脂含量 | DHA占总油脂 | DHA产量 | 发酵时间 | DHA产率 |
40g/L | 22.5g/L | 40% | 9.00g/L | 75h | 0.12g/L·h |
Claims (15)
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CN2009100334938A CN101591617B (zh) | 2009-06-15 | 2009-06-15 | 一种二十二碳六烯酸生产菌株及其诱变筛选方法和其应用 |
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CN2009100334938A CN101591617B (zh) | 2009-06-15 | 2009-06-15 | 一种二十二碳六烯酸生产菌株及其诱变筛选方法和其应用 |
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CN101591617A true CN101591617A (zh) | 2009-12-02 |
CN101591617B CN101591617B (zh) | 2011-03-23 |
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CN2009100334938A Active CN101591617B (zh) | 2009-06-15 | 2009-06-15 | 一种二十二碳六烯酸生产菌株及其诱变筛选方法和其应用 |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102839129A (zh) * | 2011-06-23 | 2012-12-26 | 法国罗凯特兄弟公司 | 一种裂殖壶菌诱变方法及其产生的变异株 |
CN105331572A (zh) * | 2015-12-08 | 2016-02-17 | 南京工业大学 | 一种发酵高产dha的方法 |
CN106047708A (zh) * | 2015-12-15 | 2016-10-26 | 湖南科技大学 | 诱变后高产淀粉酶菌株的筛选方法 |
CN108949580A (zh) * | 2018-05-18 | 2018-12-07 | 武汉藻优生物科技有限公司 | 一种高dha含量的裂壶藻及其应用 |
CN108949575A (zh) * | 2018-05-18 | 2018-12-07 | 武汉藻优生物科技有限公司 | 一种高dha含量的隐甲藻及其应用 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102911868B (zh) | 2012-09-28 | 2015-12-09 | 新奥科技发展有限公司 | 一种微生物培养基及培养方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2121986A1 (en) * | 1993-04-26 | 1994-10-27 | Daizo Takeuchi | Processes for culturing marine microalgae and producing docosahexaenoic acid using the same |
CN1264967C (zh) * | 2004-12-08 | 2006-07-19 | 中国海洋大学 | 海洋真菌裂殖壶菌ouc88的工业应用 |
CN1986822A (zh) * | 2006-12-15 | 2007-06-27 | 湖北友芝友生物科技有限公司 | 用寇氏隐甲藻发酵生产二十二碳六烯酸油脂的方法 |
-
2009
- 2009-06-15 CN CN2009100334938A patent/CN101591617B/zh active Active
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102839129A (zh) * | 2011-06-23 | 2012-12-26 | 法国罗凯特兄弟公司 | 一种裂殖壶菌诱变方法及其产生的变异株 |
WO2012175027A1 (en) * | 2011-06-23 | 2012-12-27 | Roquette Freres | Methods of mutagenesis of schizochytrium sp and variant strains produced thereof |
CN106244576A (zh) * | 2011-06-23 | 2016-12-21 | 法国罗凯特兄弟公司 | 裂殖壶菌诱变方法及其产生的变异株 |
CN105331572A (zh) * | 2015-12-08 | 2016-02-17 | 南京工业大学 | 一种发酵高产dha的方法 |
CN105331572B (zh) * | 2015-12-08 | 2018-11-20 | 南京工业大学 | 一种发酵高产dha的方法 |
CN106047708A (zh) * | 2015-12-15 | 2016-10-26 | 湖南科技大学 | 诱变后高产淀粉酶菌株的筛选方法 |
CN108949580A (zh) * | 2018-05-18 | 2018-12-07 | 武汉藻优生物科技有限公司 | 一种高dha含量的裂壶藻及其应用 |
CN108949575A (zh) * | 2018-05-18 | 2018-12-07 | 武汉藻优生物科技有限公司 | 一种高dha含量的隐甲藻及其应用 |
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