CN101579330B - disinfectant for animals and preparation method thereof - Google Patents

disinfectant for animals and preparation method thereof Download PDF

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Publication number
CN101579330B
CN101579330B CN2009100879145A CN200910087914A CN101579330B CN 101579330 B CN101579330 B CN 101579330B CN 2009100879145 A CN2009100879145 A CN 2009100879145A CN 200910087914 A CN200910087914 A CN 200910087914A CN 101579330 B CN101579330 B CN 101579330B
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China
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disinfectant
animals
glutaraldehyde
ammonium salt
quaternary ammonium
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CN101579330A (en
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肖希龙
沈建忠
汤树生
江海洋
原霖
夏娌君
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China Agricultural University
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China Agricultural University
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Abstract

The invention discloses a disinfectant for animals and a preparation method thereof, belonging to the field of medicines for veterinary use. The disinfectant for animals takes glutaric dialdehyde, single-chain quaternary ammonium salt and double-chain quaternary ammonium salt as active ingredients. The preparation method comprises the following steps: 1) adding glutaric dialdehyde, alcohol and surfactant to a container to stir uniformly and place statically; 2) adding single-chain quaternary ammonium salt and double-chain quaternary ammonium salt; 3) using a part phosphoric acid buffer solution to dissolve the chelating agent and oxidant completely and mixing with the above ingredient; and 4) continuously adding phosphoric acid buffer solution to the formulation amount to obtain a disinfectant stock solution with pH value of 2.5-4.0. The invention has low toxicity, low irritation, small smell, small corrosiveness, high efficiency of disinfection and side disinfecting spectrum. The liquid disinfectant with wide bactericidal spectrum and some washing efficiency is specifically suitable for daily disinfection of colony house, and is suitable for largely popularizing and using.

Description

Disinfectant for animals and preparation method thereof
Technical field
The present invention relates to disinfectant, be specifically related to a kind of poultry, animal house and band poultry disinfectant of being applicable to and contain compound recipe disinfectant for animals of glutaraldehyde and single, double chain quaternary ammonium salt and preparation method thereof.
Background technology
Along with developing rapidly of intensive culture industry, utilize disinfectant that poultry and surrounding thereof are carried out disinfection to kill pathogen, cut off the route of infection, become a kind of important measures of prevention and control poultry disease transmission.But at present often disinfectant of using such as bleaching powder, sodium hypochlorite, peroxide, phenols, formaldehyde etc. all have some common shortcomings, and are strong as poor stability, corrosivity, strong and stimulating etc. is arranged very, and what have is also harmful, even carcinogenesis is arranged.
Glutaraldehyde is a kind of wide spectrum, efficient, lowly stimulate, low burn into safety and low toxicity, stable disinfectant; having powerful sterilization and the effect of killing the virus the time also has brood cell's activity extremely, is unique product that the AOAC that can satisfy Environmental Protection Agency USA's strict regulations at present kills the spore requirement of experiment.But use glutaraldehyde to have certain defective separately, on the one hand be that glutaraldehyde is stable and a little less than the sterilizing ability under acid condition, strong but the stable extreme difference of bactericidal properties ability under alkali condition, need higher concentration when using glutaraldehyde separately on the other hand, and the glutaraldehyde of higher concentration has stronger zest.
Quaternary cationic surfactant has that bacteriocidal concentration is low, nonirritant, nontoxic, non-corrosive advantage, also have in addition surfactant surface adsorption, reduce capillary effect and have certain washing function.But the defective of quaternary cationic surfactant maximum is narrow antimicrobial spectrum (effective to virus, antibacterial, fungus, but invalid to mycoplasma, spore), causes it to be subjected to great restriction in the clinical use of veterinary drug.Though the uniting use and also can improve Disinfection Effect of single, double chain quaternary ammonium salt, as U.S. Pat 4464398 strand quaternary ammonium salt and strand quaternary ammonium salt are used, though be used alone quaternary ammonium salt disinfectant and compare and improved disinfection efficiency, but can not kill mycoplasma, spore because the quaternary ammonium salt bactericide spectrum is narrow, therefore can not use as broad-spectrum disinfectant.Another U.S. Pat 5322856 has mentioned that glutaraldehyde and double-chain quaternary ammonium salt unite use, and this operational version has enlarged fungicidal spectrum, but only uses the quaternary ammonium salt of single variety to make it be easy to be subjected to the Organic substance effect and lost efficacy.
Summary of the invention
The invention provides be applicable to poultry, animal house and the band poultry disinfectant that a kind of fungicidal spectrum is wide, zest is less and contain the compound recipe disinfectant for animals of glutaraldehyde and single, double chain quaternary ammonium salt.
For solving the problems of the technologies described above, the present invention takes following technical scheme:
A kind of disinfectant for animals is to be the disinfectant for animals of effective ingredient with glutaraldehyde, strand quaternary ammonium salt, double-chain quaternary ammonium salt.
Each content of effective is in the described disinfectant for animals: glutaraldehyde 1-2.5% (concentration of volume percent, L/100L), and strand quaternary ammonium salt 0.1-5% (the mass/volume percent concentration, kg/100L), double-chain quaternary ammonium salt 0.1-5% (the mass/volume percent concentration, kg/100L).
Described strand quaternary ammonium salt is preferably benzalkonium chloride.
Described double-chain quaternary ammonium salt is preferably didecyl dimethyl ammonium bromide.
The storing solution pH value of described disinfectant for animals is preferably 2.5-4.0; The phosphoric acid buffer is regulated.Be adjusted to pH7.5-8.5 with sodium carbonate or the agent of sodium bicarbonate equal excitation during use.
Also comprise following adjuvant in the described disinfectant for animals: surfactant, alcohol, antioxidant and chelating agen.
Described surfactant is preferably non-ionic surface active agent, can be selected from one or more mixture in alkylphenol polyoxyethylene, sorbitan monooleate polyoxyethylene ether, polyoxyethylene laurel ether and the fatty alcohol-polyoxyethylene ether etc.
Described alcohol can be selected from ethanol, propanol, isopropyl alcohol, ethylene glycol, 1,2-glycerol, 1,2-propylene glycol, 1, one or more mixture in ammediol, triethylene glycol and the diethylene glycol etc.
Described antioxidant can be selected from one or more mixture in ascorbic acid, arabo-ascorbic acid, sodium ascorbate, sodium pyrosulfite, propyl gallate and the sodium sulfite etc.
Described chelating agen is preferably EDTA or its sodium salt.
Specifically, the content of each composition is in the described disinfectant for animals: glutaraldehyde 1-2.5% (L/100L), strand quaternary ammonium salt 0.1-5% (kg/100L), double-chain quaternary ammonium salt 0.1-5% (kg/100L), surfactant 0.1-5% (L/100L), pure 1-20% (L/100L), antioxidant 0.01-0.1% (kg/100L), chelating agen 0.01-0.1% (kg/100L), pH 2.5-4.0.
Second purpose of the present invention provides a kind of method for preparing above-mentioned disinfectant for animals.
Preparation method provided by the present invention can may further comprise the steps:
1) glutaraldehyde, alcohol, surfactant are added container and stir, make glutaraldehyde, isopropyl alcohol and surfactant mix homogeneously and dissolving fully, leave standstill;
2) add strand quaternary ammonium salt and double-chain quaternary ammonium salt;
3) with part phosphoric acid buffer solution chelating agen and oxidant being dissolved the back fully mixes with aforementioned component;
4) continue to add phosphate buffer to formula ratio and obtain the disinfectant storing solution that pH value is 2.5-4.0.
The invention provides a kind of disinfectant for animals and preparation method thereof.This liquid disinfectant be by glutaraldehyde and single, double chain quaternary ammonium salt as active component, be aided with pharmaceutic adjuvants such as non-ionic surface active agent, alcohol, chelating agen, antioxidant, the disinfectant of a kind of stable, efficient, the low concentration that is prepared into through scientific composition.The present invention has successfully developed three's compound disinfectant by the rational proportion that big quantity research filters out glutaraldehyde, strand quaternary ammonium salt and double-chain quaternary ammonium salt, make between each composition and produce synergism, improved germicidal efficiency, enlarged fungicidal spectrum, make this disinfectant killing action all be arranged, also make this product can be widely used in the sterilization of various places, various uses virus, antibacterial, fungus, mycoplasma, spore.The advantage of this disinfectant is not only to have avoided independent use glutaraldehyde to need higher concentration and inevitable zest, overcome quaternary ammonium salt again and can not kill the defective of mycoplasma, spore, and strand quaternary ammonium salt and double-chain quaternary ammonium salt use simultaneously and also improved quaternary ammonium salt and resist organic ability.Low toxicity of the present invention, low irritant, abnormal smells from the patient is little, corrosivity is little and have the disinfection efficiency height, and the wide and liquid disinfectant that have certain washing functions of fungicidal spectrum is specially adapted to the daily sterilization and the sterilization of band poultry of poultry colony house, suits large area to popularize and uses.
Below in conjunction with specific embodiment the present invention is described in further details.
The specific embodiment
The present invention is national Eleventh Five-Year Plan science and technology support plan problem (problem title: the development of animal compound novel formulation and application; Project number: partial content 2006BAD31B08).
The invention provides a kind of compound recipe disinfectant for animals that contains glutaraldehyde and single, double chain quaternary ammonium salt.In compound recipe assembly process, need the special concertedness of considering between each composition, and form the preparation that effect is remarkable and have good stability by screening appropriate adjuvant.
This liquid disinfectant be by glutaraldehyde and single, double chain quaternary ammonium salt as active component, be aided with pharmaceutic adjuvants such as non-ionic surface active agent, alcohol, chelating agen, antioxidant, the disinfectant of a kind of stable, efficient, the low concentration that is prepared into through scientific composition.
Glutaraldehyde is a kind of sporicide, have very powerful sterilization and spore effect, but the glutaraldehyde of high concentration has certain zest, and the glutaraldehyde of 1-2.5% (L/100L) both can keep it to kill the spore performance, reduced its zest again.With quaternary ammonium salt and glutaraldehyde compatibility, the bactericidal action of glutaraldehyde can be strengthened among the present invention, the activity of glutaraldehyde can be further reduced again.Stable under acid condition because of glutaraldehyde among the present invention, the bactericidal properties ability is strong under the alkali condition, so the present invention selects storing solution under the acid condition of pH2.5-4.0, the reuse activator makes medicine reach performance bactericidal action under the alkali condition in use.Can be used for sodium carbonate, sodium bicarbonate etc. being arranged with the activator of disinfectant effect of the present invention.
Quaternary ammonium salt is a cationic surfactant, and it is deactivation gram positive bacteria, negative bacterium effectively, but invalid to mycobacterium and fungus.All be vulnerable to the influence of microbe species, Organic substance and hard water when cationic surfactant uses separately, the disinfectant quaternary ammonium salt composite with glutaraldehyde can reduce surface tension and have certain washing function can play the effect of Synergistic again with glutaraldehyde.Select strand quaternary ammonium salt and double-chain quaternary ammonium salt among the present invention simultaneously, experiment shows that the mixture bactericidal effect of strand quaternary ammonium salt and double-chain quaternary ammonium salt is better than single variety, and can strengthen its anti-interference.Wherein, the preferred benzalkonium chloride of strand quaternary ammonium salt, concentration is 0.1-5% (kg/100L), the preferred didecyl dimethyl of double-chain quaternary ammonium salt ammonium bromide, its concentration is 0.1-5% (kg/100L).
Non-ionic surface active agent has permeability that reduces surface tension, strengthens glutaraldehyde and the effect that improves the glutaraldehyde disinfection efficiency.Non-ionic surface active agent among the present invention adopts one or more mixture in alkylphenol polyoxyethylene (OP), sorbitan monooleate polyoxyethylene ether (TWEEN), polyoxyethylene laurel ether (BRIJ35), the fatty alcohol-polyoxyethylene ether materials such as (AEO9), and consumption is 0.1-5% (L/100L).
Alcohol itself has certain antibacterial action, the used alcohol of the present invention is the straight chain water-soluble alcohol, comprise ethanol, propanol, isopropyl alcohol, ethylene glycol, 1,2-glycerol, 1,2-propylene glycol, 1, one or more mixture in the materials such as ammediol, triethylene glycol, diethylene glycol, consumption is 1-20% (L/100L).
Chelating agen can with heavy metal ion chelating in the water, reduce the influence of heavy metal ion to the disinfectant effect.So the present invention's chelating agen can be EDTA or its sodium salt, content 0.01-0.1% (kg/100L).
Antioxidant can react with free oxygen, avoids glutaraldehyde oxidized, thereby plays the effect of stablizing glutaraldehyde.The used antioxidant of the present invention can be one or more mixture in the materials such as ascorbic acid, arabo-ascorbic acid, sodium ascorbate, sodium pyrosulfite, propyl gallate, sodium sulfite, content 0.01-0.1% (kg/100L).
It is that 2.5-4.0 becomes storing solution that this disinfectant transfers to pH with buffer, adds activator during use and makes pH value reach 7.5-8.5.Institute's water is deionized water or distilled water among the present invention.
Below further specify the present invention with specific embodiment.Following embodiment 1-15 adopts best experimental program of the present invention, and those of ordinary skill in the art can expect some identical, replacement schemes apparently according to content disclosed by the invention, all should belong to disclosure of the present invention.Method therefor is conventional method if no special instructions among the embodiment.
The preparation of embodiment 1, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 1.6%, i.e. 16g
The last of the ten Heavenly stems first bromine ammonium 2.4%, i.e. 24g
BRIJ35 0.25%, i.e. 2.5mL
Isopropyl alcohol 15%, i.e. 150mL
EDTA-2Na 0.1%, i.e. 1g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol and polyoxyethylene laurel ether (BRIJ35) are added container and stir, make glutaraldehyde, isopropyl alcohol and polyoxyethylene laurel ether mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide (first bromine ammonium in the last of the ten Heavenly stems);
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH2.5.
The preparation of embodiment 2, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 0.2%, i.e. 2g
The last of the ten Heavenly stems first bromine ammonium 0.3%, i.e. 3g
BRIJ35 0.25%, i.e. 2.5mL
Isopropyl alcohol 15%, i.e. 150mL
Ethylene glycol 2%, i.e. 20mL
EDTA-2Na 0.1%, i.e. 1g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol, ethylene glycol and polyoxyethylene laurel ether (BRIJ35) are added container and stir, make glutaraldehyde, isopropyl alcohol, ethylene glycol and polyoxyethylene laurel ether mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.5.
The preparation of embodiment 3, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 1%, i.e. 10mL
Benzalkonium chloride 2.8%, i.e. 28g
The last of the ten Heavenly stems first bromine ammonium 1.4%, i.e. 14g
BRIJ35 0.25%, i.e. 2.5mL
Isopropyl alcohol 10%, i.e. 100mL
1, the 2-glycerol 1%, i.e. 10mL
EDTA-2Na 0.1%, i.e. 1g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) with glutaraldehyde, isopropyl alcohol, 1,2-glycerol and polyoxyethylene laurel ether (BRIJ35) add container and stir, and make glutaraldehyde, isopropyl alcohol, 1, and 2-glycerol and polyoxyethylene laurel ether mix homogeneously and dissolving are fully left standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH4.0.
The preparation of embodiment 4, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2.5%, i.e. 25mL
Benzalkonium chloride 5%, i.e. 50g
The last of the ten Heavenly stems first bromine ammonium 5%, i.e. 50g
BRIJ35 0.25%, i.e. 2.5mL
Triethylene glycol 4.5%, i.e. 45mL
EDTA-2Na 0.1%, i.e. 1g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, triethylene glycol and polyoxyethylene laurel ether are added container and stir, make glutaraldehyde, triethylene glycol and polyoxyethylene laurel ether mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.0.
The preparation of embodiment 5, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 2.8%, i.e. 28g
The last of the ten Heavenly stems first bromine ammonium 1.4%, i.e. 14g
AEO9 0.25%, i.e. 2.5mL
Isopropyl alcohol 4.5%, i.e. 45mL
EDTA-2Na 0.1%, i.e. 1g
Ascorbic acid 0.05%, i.e. 0.5g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol and fatty alcohol-polyoxyethylene ether (AEO9) are added container and stir, make glutaraldehyde, isopropyl alcohol and AEO9 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.0.
The preparation of embodiment 6, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 1.5%, i.e. 15mL
Benzalkonium chloride 0.1%, i.e. 1g
The last of the ten Heavenly stems first bromine ammonium 0.1%, i.e. 1g
AEO9 0.25%, i.e. 2.5mL
Isopropyl alcohol 15%, i.e. 150mL
EDTA-2Na 0.1%, i.e. 1g
Ascorbic acid 0.01%, i.e. 0.1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol and AEO9 are added container and stir, make glutaraldehyde, isopropyl alcohol and AEO9 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.5.
The preparation of embodiment 7, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 2.8%, i.e. 28g
The last of the ten Heavenly stems first bromine ammonium 1.4%, i.e. 14g
AEO9 0.25%, i.e. 2.5mL
Isopropyl alcohol 15%, i.e. 150mL
1, ammediol 2.5%, i.e. 25mL
Diethylene glycol 2.5%, i.e. 25mL
EDTA-2Na 0.1%, i.e. 1g
Arabo-ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol and AEO9 are added container and stir, make glutaraldehyde, isopropyl alcohol and AEO9 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and arabo-ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.5.
The preparation of embodiment 8, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 2.8%, i.e. 28g
The last of the ten Heavenly stems first bromine ammonium 1.4%, i.e. 14g
AEO9 2%, i.e. 20mL
1, the 2-propylene glycol 1%, i.e. 10mL
EDTA-2Na 0.1%, i.e. 1g
Sodium ascorbate 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, triethylene glycol and AEO9 are added container and stir, make glutaraldehyde, triethylene glycol and AEO9 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and sodium ascorbate mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.5.
The preparation of embodiment 9, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 1.6%, i.e. 16g
The last of the ten Heavenly stems first bromine ammonium 2.4%, i.e. 24g
AEO9 0.25%, i.e. 2.5mL
Triethylene glycol 4.5%, i.e. 45mL
EDTA-2Na 0.1%, i.e. 1g
Propyl gallate 0.05%, i.e. 0.5g
Ascorbic acid 0.05%, i.e. 0.5g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, triethylene glycol and AEO9 are added container and stir, make glutaraldehyde, triethylene glycol and AEO9 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid, propyl gallate mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH4.0.
The preparation of embodiment 10, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 0.2%, i.e. 2g
The last of the ten Heavenly stems first bromine ammonium 0.3%, i.e. 3g
AEO9 0.1%, i.e. 1mL
Triethylene glycol 4.5%, i.e. 45mL
EDTA-2Na 0.1%, i.e. 1g
Sodium sulfite 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps: 1) glutaraldehyde, triethylene glycol and AEO9 are added container and stir, make glutaraldehyde, triethylene glycol and AEO9 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and sodium sulfite mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.5.
The preparation of embodiment 11, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 1.6%, i.e. 16g
The last of the ten Heavenly stems first bromine ammonium 2.4%, i.e. 24g
BRIJ35 0.25%, i.e. 2.5mL
Isopropyl alcohol 10%, i.e. 100mL
EDTA 0.1%, i.e. 1g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol and BRIJ35 are added container and stir, make glutaraldehyde, isopropyl alcohol and BRIJ35 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.0.
The preparation of embodiment 12, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 0.2%, i.e. 2g
The last of the ten Heavenly stems first bromine ammonium 0.3%, i.e. 3g
BRIJ35 0.1%, i.e. 1mL
Isopropyl alcohol 10%, i.e. 100mL
EDTA 0.05%, i.e. 0.5g
EDTA-2Na 0.05%, i.e. 0.5g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol and BRIJ35 are added container and stir, make glutaraldehyde, isopropyl alcohol and BRIJ35 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA, EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH2.8.
The preparation of embodiment 13, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 2.8%, i.e. 28g
The last of the ten Heavenly stems first bromine ammonium 1.4%, i.e. 14g
Alkylphenol polyoxyethylene 0.25%, i.e. 2.5mL
BRIJ35 0.25%, i.e. 2.5mL
Isopropyl alcohol 10%, i.e. 100mL
EDTA-2Na 0.01%, i.e. 0.1g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, isopropyl alcohol and BRIJ35, alkylphenol polyoxyethylene (OP) are added container and stir, make glutaraldehyde, isopropyl alcohol and BRIJ35, alkylphenol polyoxyethylene mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH3.2.
The preparation of embodiment 14, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 0.2%, i.e. 2g
The last of the ten Heavenly stems first bromine ammonium 0.3%, i.e. 3g
BRIJ35 0.25%, i.e. 2.5mL
Ethanol 4.5%, i.e. 45mL
EDTA-2Na 0.1%, i.e. 1g
Ascorbic acid 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, triethylene glycol and BRIJ35 are added container and stir, make glutaraldehyde, triethylene glycol and BRIJ35 mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH2.9.
The preparation of embodiment 15, disinfectant for animals
The prescription of disinfectant for animals of the present invention following (1L):
Component Content
Glutaraldehyde 2%, i.e. 20mL
Benzalkonium chloride 1.6%, i.e. 16g
The last of the ten Heavenly stems first bromine ammonium 2.4%, i.e. 24g
TWEEN 4.75%, i.e. 47.5mL
BRIJ35 0.25%, i.e. 2.5mL
Triethylene glycol 4.5%, i.e. 45mL
EDTA-2Na 0.1%, i.e. 1g
Sodium ascorbate 0.1%, i.e. 1g
Phosphate buffer Add to 1L.
The preparation method of disinfectant for animals provided by the present invention can may further comprise the steps:
1) glutaraldehyde, triethylene glycol and BRIJ35, TWEEN are added container and stir, make glutaraldehyde, triethylene glycol and BRIJ35, TWEEN mix homogeneously and dissolving fully, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) to be 3.0 phosphate buffer dissolve the back fully with EDTA-2Na and ascorbic acid mixes with aforementioned component with the part pH value;
4) continuing to add phosphate buffer to cumulative volume is 1L, obtains the disinfectant for animals storing solution of pH4.0.
The Disinfection Effect of testing a disinfectant for animals of the present invention detects test
One, experimental technique
Test with reference to " disinfectant for animals qualification test technical specification " (Ministry of Agriculture) method, adopt the bacterial suspension quantitative method, test strain has escherichia coli 8099, staphylococcus aureus ATCC 6538, bacillus cereus 8008 spore.Concrete experimental procedure is as follows:
1, the bacterium liquid with preparation carries out count plate, is diluted to the phosphate buffer of 0.03M then to contain bacterium 10 6～10 7The test organisms liquid of individual/ml.
2, draw 0.1ml bacterium liquid in the disinfectant solution 0.9ml of test concentrations.
3, put in 20 ℃ of water-baths and act on to the stipulated time.
4, draw above-mentioned bacterium medicine mixed liquor 0.1ml immediately, mixing is as specimen in the adding 0.9ml nertralizer, and nertralizer is for containing the distilled water solution of 2% glycine (W/V), 3% lecithin (W/V), 10% sodium thiosulfate (W/V), 10% tween 80 (V/V).
5, the phosphate buffer with 0.03M replaces disinfectant solution, carries out above-mentioned steps 1-4 simultaneously, organizes sample in contrast.
6, will carry out count plate in the sample of step 4 and 5 and after 10 minutes, calculate sterilizing rate.
Sterilizing rate=(matched group bacterium number-sample sets bacterium number)/matched group bacterium number * 100%.
Two, disinfectant for animals of the present invention is to the colibacillary result of the test of killing
Disinfectant for animals of the present invention to colibacillary kill test the results are shown in Table 1, from table 1 data as can be known,, just can reach the sterilization requirement at 1 minute during the dilution in 1: 1500 of this disinfectant for escherichia coli 8099, sterilizing rate is more than 99.9%.1: 2000 when dilution, needed 5 minutes reach the sterilization requirement, and during dilution in 1: 2500 at 10 minutes with the interior sterilizing rate that does not reach regulation.
Table 1 disinfectant for animals of the present invention is to colibacillary average bactericidal rate
Figure G2009100879145D00131
Matched group bacterium number is 1.57 * 10 7Cfu/ml
Three, disinfectant for animals of the present invention is to the result of the test of killing of staphylococcus aureus
Disinfectant for animals of the present invention to staphylococcus aureus kill test the results are shown in Table 2, from table 2 data as can be known, for staphylococcus aureus ATCC 6538, just can reach the sterilization requirement at 1 minute during the dilution in 1: 1000 of this disinfectant, sterilizing rate is more than 99.9%.1: 2000 when dilution, needed 5 minutes reach the sterilization requirement, and during dilution in 1: 2500 at 10 minutes with the interior sterilizing rate that does not reach regulation.This disinfectant is to the sterilizing ability basically identical of escherichia coli and staphylococcus aureus.
Table 2 disinfectant for animals of the present invention is to the average bactericidal rate of staphylococcus aureus
Figure G2009100879145D00141
Matched group bacterium number is 9.5 * 10 7Cfu/ml
Four, disinfectant for animals of the present invention is to the test of killing of wax-shaped bacillus spore
Disinfectant for animals of the present invention to the bacillus cereus spore kill test the results are shown in Table 3, from table 3 data as can be known, for the bacillus cereus spore, the dilution in 1: 2 of this disinfectant reached the regulation bactericidal effect in 1 hour, dilution in 1: 4 then needed meet the demands in 2 hours.The data of consolidated statement 1, table 2, table 3, disinfectant of the present invention as can be known is all effective to bacterial spore, is a kind of broad-spectrum disinfectant.
Table 3 disinfectant for animals of the present invention is to the average bactericidal rate of wax-shaped bacillus spore
Figure G2009100879145D00142
Matched group bacterium number is 1.57 * 10 7Cfu/ml
Five, test is killed in the contrast of disinfectant for animals of the present invention and other broad-spectrum disinfectants
Two kinds of contrast disinfectant are respectively: go up the hundred malicious Kill S that the biochemical company limited of this moral of the Shanghai's style produces (10% last of the ten Heavenly stems first bromine ammonium salt solution), the peace of Schering Plough animal health product company limited production is killed (15% glutaraldehyde and 10% patent COCO quaternary amine).The active component of other two kinds of disinfectant is diluted to identical, and then is diluted to working concentration in proportion, other experiment condition unanimity with this disinfectant stock solution.Comparative test result sees Table 4 and table 5.
Experimental result is killed in the contrast of table 4 staphylococcus aureus
Figure G2009100879145D00151
Matched group bacterium number is 5.29 * 10 7Cfu/ml
Experimental result is killed in the contrast of table 5 wax-shaped bacillus spore
Figure G2009100879145D00152
Matched group bacterium number is 5.19 * 10 7Cfu/ml
Annotate: the original liquid concentration that peace is killed refers to the concentration of 15% glutaraldehyde dilution cost disinfectant glutaraldehyde in the commercially available back is tested again; The original liquid concentration of hundred malicious Kill refers to the concentration of first bromine ammonium dilution in last of the ten Heavenly stems of 10% cost disinfectant quaternary ammonium salt is tested again.
As can be seen from Table 4, take the staphylococcus aureus as the representative of antibacterial indicator bacteria, three kinds of disinfectant killing effect difference when dilution in 1: 1000 is little, but disinfectant effect of the present invention is best, and sterilizing rate reached 99.999% in 1 minute.When dilution in 1: 2500, hundred malicious Kill bactericidal effects are relatively poor, can not reach requirement.As can be seen from Table 5, this disinfectant to kill the spore effect best, peace is killed slightly and is taken second place, the bactericidal effect of hundred malicious Kill is relatively poor.
In sum, this disinfectant is all effective to bacterial spore, is a kind of broad-spectrum disinfectant, and better than two kinds of sanitizer disinfecting effects commonly used in the market.
Test two glutaraldehydes and single, double chain quaternary ammonium salt is united the sterilization contrast experiment of use
Test method is divided into glutaraldehyde, double-chain quaternary ammonium salt, strand quaternary ammonium salt, double-chain quaternary ammonium salt and the coupling of strand quaternary ammonium salt, glutaraldehyde and 5 experimental grouies of single, double chain quaternary ammonium salt coupling with test one.Wherein the concentration of single quaternary ammonium salt is identical with the total concentration of two kinds of quaternary ammonium salt couplings, and the concentration of one pack system glutaraldehyde is identical with the glutaraldehyde concentration of coupling group, and then is diluted to working concentration in proportion.Remove the sterilization component difference, other reaction condition is all consistent.The results are shown in Table 6 and table 7.
Table 6 different component disinfectant is to the experimental result of killing of staphylococcus aureus
Matched group bacterium number is 1.18 * 10 8Cfu/ml
As can be seen from Table 6, glutaraldehyde does not have bactericidal action in 5 minutes after vast scale dilution.The bactericidal effect of double-chain quaternary ammonium salt and strand quaternary ammonium salt is basic identical, and the effect after the coupling plays synergism.When glutaraldehyde and two kinds of quaternary ammonium salt couplings, after vast scale dilution, glutaraldehyde is very little to the killing action of antibacterial, mainly be quaternary ammonium salt in action.So when kill bacteria, use the glutaraldehyde bactericidal effect very poor separately, glutaraldehyde and quaternary ammonium salt coupling have then improved bactericidal effect greatly.
Table 7 different component disinfectant is to the experimental result of killing of wax-shaped bacillus spore
Figure G2009100879145D00171
Matched group bacterium number is 1.35 * 10 8Cfu/ml
As can be seen from Table 7, the 4%W/V quaternary ammonium salt one hour with interior very poor to the spore effect, but double-chain quaternary ammonium salt and strand quaternary ammonium salt coupling meeting make and kill the spore effect and strengthen.And the coupling of glutaraldehyde and quaternary ammonium salt can be strengthened the spore effect extremely of glutaraldehyde.
Based on the above results, illustrate that glutaraldehyde and single, double chain quaternary ammonium salt are united use can enlarge fungicidal spectrum, can kill bacteria, can kill spore again.The coupling of single, double chain quaternary ammonium salt produces synergistic function, strengthens the ability that glutaraldehyde is killed spore simultaneously, can reduce the working concentration of glutaraldehyde, reduces zest.

Claims (7)

1. disinfectant for animals, by the pH value of regulating with phosphate buffer is that the storing solution of 2.5-4.0 and the exciting agent used when using are formed, the effective ingredient that comprises following content in the described storing solution: glutaraldehyde 1-2.5L/100L, benzalkonium chloride 0.1-5kg/100L, didecyl dimethyl ammonium bromide 0.1-5kg/100L; Described exciting agent is sodium carbonate or sodium bicarbonate, during use storing solution is adjusted to pH7.5-8.5.
2. disinfectant for animals according to claim 1, it is characterized in that: the surfactant that also comprises 0.1-5L/100L in the storing solution, described surfactant is a non-ionic surface active agent, is selected from one or more mixture in alkylphenol polyoxyethylene, sorbitan monooleate polyoxyethylene ether, polyoxyethylene laurel ether and the fatty alcohol-polyoxyethylene ether.
3. disinfectant for animals according to claim 1, it is characterized in that: the alcohol that also comprises 1-20L/100L in the storing solution, described alcohol is selected from ethanol, propanol, isopropyl alcohol, ethylene glycol, 1,2-propylene glycol, 1, one or more mixture in ammediol, triethylene glycol and the diethylene glycol.
4. disinfectant for animals according to claim 1, it is characterized in that: also comprise the antioxidant of 0.01-0.1kg/100L in the storing solution, described antioxidant is selected from one or more mixture in ascorbic acid, arabo-ascorbic acid, sodium ascorbate, calcium ascorbate, sodium pyrosulfite, propyl gallate and the sodium sulfite.
5. disinfectant for animals according to claim 1 is characterized in that: also comprise the chelating agen of 0.01-0.1kg/100L in the storing solution, described chelating agen is EDTA or its sodium salt.
6. disinfectant for animals according to claim 1, it is characterized in that: the content of each composition is in the storing solution of described disinfectant for animals: glutaraldehyde 1-2.5L/100L, benzalkonium chloride 0.1-5kg/100L, didecyl dimethyl ammonium bromide 0.1-5kg/100L, surfactant 0.1-5L/100L, alcohol 1-20L/100L, antioxidant 0.01-0.1kg/100L, chelating agen 0.01-0.1kg/100L; Described surfactant is a non-ionic surface active agent, is selected from one or more mixture in alkylphenol polyoxyethylene, sorbitan monooleate polyoxyethylene ether, polyoxyethylene laurel ether and the fatty alcohol-polyoxyethylene ether; Described alcohol is selected from ethanol, propanol, isopropyl alcohol, ethylene glycol, 1,2-propylene glycol, 1, one or more mixture in ammediol, triethylene glycol and the diethylene glycol; Described antioxidant is selected from one or more mixture in ascorbic acid, arabo-ascorbic acid, sodium ascorbate, calcium ascorbate, sodium pyrosulfite, propyl gallate and the sodium sulfite; Described chelating agen is EDTA or its sodium salt.
7. method for preparing the described disinfectant for animals of claim 6 may further comprise the steps:
1) glutaraldehyde, alcohol, surfactant are added container and stir, leave standstill;
2) add benzalkonium chloride and didecyl dimethyl ammonium bromide;
3) with part phosphoric acid buffer solution chelating agen and antioxidant being dissolved the back fully mixes with aforementioned component;
4) continue to add phosphate buffer to formula ratio and obtain the disinfectant for animals storing solution that pH value is 2.5-4.0.
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