CN115530166B - Composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide and preparation method thereof - Google Patents
Composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide and preparation method thereof Download PDFInfo
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- CN115530166B CN115530166B CN202211247480.2A CN202211247480A CN115530166B CN 115530166 B CN115530166 B CN 115530166B CN 202211247480 A CN202211247480 A CN 202211247480A CN 115530166 B CN115530166 B CN 115530166B
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- 239000000645 desinfectant Substances 0.000 title claims abstract description 78
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 239000002131 composite material Substances 0.000 title claims abstract description 32
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title abstract description 13
- GTQHJCOHNAFHRE-UHFFFAOYSA-N 1,10-dibromodecane Chemical compound BrCCCCCCCCCCBr GTQHJCOHNAFHRE-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- -1 polyoxyethylene Polymers 0.000 claims description 18
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims description 7
- 239000005977 Ethylene Substances 0.000 claims description 7
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 claims description 6
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 6
- SWLVFNYSXGMGBS-UHFFFAOYSA-N ammonium bromide Chemical compound [NH4+].[Br-] SWLVFNYSXGMGBS-UHFFFAOYSA-N 0.000 claims description 6
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims description 6
- 229920001451 polypropylene glycol Polymers 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 239000012747 synergistic agent Substances 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 150000002894 organic compounds Chemical class 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 35
- 230000001954 sterilising effect Effects 0.000 abstract description 22
- 230000000694 effects Effects 0.000 abstract description 19
- 241000894006 Bacteria Species 0.000 description 15
- 238000012360 testing method Methods 0.000 description 11
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 8
- 239000008367 deionised water Substances 0.000 description 8
- 229910021641 deionized water Inorganic materials 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 241000700605 Viruses Species 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- 239000004094 surface-active agent Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 230000001133 acceleration Effects 0.000 description 3
- 230000007797 corrosion Effects 0.000 description 3
- 238000005260 corrosion Methods 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004215 Carbon black (E152) Substances 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 150000003863 ammonium salts Chemical group 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- GZUXJHMPEANEGY-UHFFFAOYSA-N bromomethane Chemical compound BrC GZUXJHMPEANEGY-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical group 0.000 description 2
- 125000001165 hydrophobic group Chemical group 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 150000002500 ions Chemical group 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000005060 rubber Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- FNXLCIKXHOPCKH-UHFFFAOYSA-N bromamine Chemical compound BrN FNXLCIKXHOPCKH-UHFFFAOYSA-N 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 229940102396 methyl bromide Drugs 0.000 description 1
- XEUYTSFFGRRIIU-UHFFFAOYSA-N n-methyldecan-1-amine;hydrobromide Chemical compound Br.CCCCCCCCCCNC XEUYTSFFGRRIIU-UHFFFAOYSA-N 0.000 description 1
- 239000010816 packaging waste Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Toxicology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a composite veterinary disinfectant containing glutaraldehyde and decamethylene bromide and a preparation method thereof, which relate to the technical field of veterinary disinfectants and comprise the following raw materials in percentage by mass: the disinfectant disclosed by the invention has the advantages that the sterilization effect of the glutaraldehyde decamethy bromide disinfectant is improved, the property of the disinfectant is not influenced, and the disinfectant is stable in a low-temperature environment (-20 ℃ to-18 ℃).
Description
Technical Field
The invention relates to the technical field of veterinary disinfectants, in particular to a composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide and a preparation method thereof.
Background
Glutaraldehyde decamethylene bromide solution is a high-efficiency broad-spectrum disinfectant, but the preparation of the conventional prescription at present has defects.
Chinese patent application CN201810637946.7 discloses a glutaraldehyde decamethylene bromide solution and a preparation method thereof, and the main components of the disinfectant are glutaraldehyde and decamethylene bromide, and the disinfectant has the characteristics of no pungent smell, high stability and long-term storage, but the problem of low-temperature failure easily occurs because the use of low-temperature conditions is not considered.
Chinese patent application CN201911421388.1 discloses a glutaraldehyde decamethylene bromide compound disinfectant suitable for low-temperature use, wherein the disinfectant consists of glutaraldehyde, decamethylene bromide, a deicing agent and a solvent, the deicing agent adopts ethanol, glycol and sodium chloride, but the ethanol and the glycol are volatile, and the glycol is toxic, so that the problem of metal corrosion cannot be thoroughly solved when the ethanol, the glycol and the sodium chloride are used.
Chinese patent application No. CN112568220.A is glutaraldehyde decamethyhammonium bromide solution, its preparation method and application, and stability of the solution is affected after adding AEO surfactant.
The above patents do not really solve the synergy problem of the disinfectant, and have a great gap with the disinfection effect of foreign similar preparations.
Disclosure of Invention
Aiming at the problems of weak sterilization effect, unstable storage, low-temperature failure and the like of glutaraldehyde and decamethylene bromide disinfectant under the current background, the invention aims to provide the compound veterinary disinfectant containing glutaraldehyde and decamethylene bromide and the preparation method thereof, so that the sterilization effect of the glutaraldehyde decamethylene bromide disinfectant is improved, and the properties of the disinfectant are not influenced.
In order to achieve the above object, the present invention provides the following solutions:
The composite veterinary disinfectant containing glutaraldehyde and decamethylene bromide comprises the following raw materials in percentage by mass: 1 to 15 percent of glutaraldehyde, 1 to 10 percent of decamethyiammonium bromide, 0.1 to 1 percent of synergistic agent, 5 to 20 percent of ethanol, 6 to 20 percent of glycerol and the balance of water.
The invention introduces the hyperbranched gemini quaternary ammonium salt into the composite veterinary disinfectant containing glutaraldehyde and decamethy bromide, thereby greatly improving the disinfection effect (which can be improved by more than 4 times) and having no influence on the properties of the disinfectant.
Aiming at the problem of inconvenient low-temperature storage of glutaraldehyde decamethylene bromide disinfectant, the invention adds ethanol and glycerol which are non-toxic and have no influence on the environment into glutaraldehyde decamethylene bromide disinfectant, so that the glutaraldehyde decamethylene bromide disinfectant can maintain remarkable sterilization effect at low temperature.
Furthermore, the synergistic agent hyperbranched chain gemini quaternary ammonium salt used in the invention is formed by connecting two single-chain single-head group common surfactants at the ion head group through chemical bonding, so that the head group separating force of the surfactant (decamethy bromide) in the ordered aggregation process is suppressed, the surface activity is greatly improved, the action on the biological film of bacteria and viruses is enhanced, and the disinfection capacity of the disinfectant is enhanced. The hyper-branched gemini quaternary ammonium salt is preferably a hydrocarbon single chain with the carbon number of 8-16, and has the strongest disinfection synergistic effect.
Further, the synergistic agent is hyperbranched gemini quaternary ammonium salt, the model is 31627, the chemical name is ethylene bis (dodecyl polyoxyethylene/polyoxypropylene block group ammonium bromide), and the structural formula is
The preparation method of the composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide comprises the following steps:
(1) Weighing all raw material components according to the formula, mixing decamethyianium bromide and hyperbranched chain gemini quaternary ammonium salt, and stirring until the mixture is completely dissolved to obtain a first solution;
(2) Mixing glutaraldehyde, ethanol, glycerol and water uniformly to obtain a second solution;
(3) And (3) adding the first solution in the step (1) into the second solution in the step (2), and stirring until the first solution is completely dissolved to obtain the water-soluble organic compound.
The composite veterinary disinfectant containing glutaraldehyde and decamethylene bromide can be widely applied to the disinfection of surfaces of metal, wood, plastic, rubber and the like, is particularly suitable for the disinfection of farms, public places and equipment and can maintain remarkable sterilization effect at low temperature.
The invention discloses the following technical effects:
1. The composite high-efficiency disinfectant containing glutaraldehyde and decamethy bromide has the advantages of remarkably enhanced disinfection effect (more than 4 times), safety, no toxicity, no stimulation, no corrosion and small smell;
2. The ethanol and the glycerol are added, so that the composite high-efficiency disinfectant containing glutaraldehyde and decamethyiammonium bromide still has stable properties in a low-temperature environment (-20 ℃ to-18 ℃);
3. The decylmethylammonium bromide and the hyperbranched chain gemini quaternary ammonium salt are mixed in advance, so that the influence on other components is small;
4. The invention has higher concentration and remarkable synergy, can be diluted by more times when in use, and can be applied to farms after being diluted by less products by farmers, thereby not only greatly reducing the production cost of enterprises, but also reducing the use cost, simultaneously reducing the use amount of packing materials, relieving the recycling pressure of packaging waste, being beneficial to environmental protection and having wide application prospect;
5. The composite high-efficiency disinfectant containing glutaraldehyde and decamethylene bromide has the advantages of stable property, outstanding antibacterial property, low environmental impact, safety, no toxicity, no stimulation, no corrosion, small smell, old effectiveness at low temperature and the like, can be widely applied to the disinfection of surfaces of metals, wood, plastics, rubber and the like, and is particularly suitable for disinfection of farms, public places and equipment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 shows the effect of the sterilization experiment of the composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide of example 1;
FIG. 2 shows the effect of the sterilization experiment of the composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide in example 2;
FIG. 3 shows the effect of the sterilization experiment of the composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide of example 3;
FIG. 4 shows the effect of the sterilization experiment of the composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide of example 4;
FIG. 5 shows the state of a composite veterinary disinfectant sample containing glutaraldehyde and decamethenamine in examples 1-4, and examples 1,2,3, and 4 in this order from left to right, when frozen at-20deg.C for 50 days;
In fig. 1 to 4, test tube numbers A, B, C, D represent examples 1,2, 3, and 4, respectively; subscript 12345 is 1: 400. 1: 800. 1:1600;1:3200;1:6400, for example, reference A 1 in FIG. 1, indicates that the disinfectant of example 1 is 400-fold diluted.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
The embodiment of the invention provides a composite veterinary disinfectant containing glutaraldehyde and decamethylene bromide, which comprises the following raw materials in percentage by mass: 1 to 15 percent of glutaraldehyde, 1 to 10 percent of decamethyiammonium bromide, 0.1 to 1 percent of hyperbranched gemini quaternary ammonium salt, 5 to 20 percent of ethanol, 6 to 20 percent of glycerol and the balance of water.
The invention introduces the hyperbranched gemini quaternary ammonium salt into the composite veterinary disinfectant containing glutaraldehyde and decamethy bromide, thereby greatly improving the disinfection effect (more than 4 times) and having no influence on the properties of the disinfectant.
Aiming at the problem of inconvenient low-temperature storage of glutaraldehyde decamethylene bromide disinfectant, the invention adds ethanol and glycerol which are non-toxic and have no influence on the environment into glutaraldehyde decamethylene bromide disinfectant, so that the glutaraldehyde decamethylene bromide disinfectant can maintain remarkable sterilization effect at low temperature.
In the embodiment of the invention, the hyperbranched chain gemini quaternary ammonium salt is formed by connecting two single-chain single-head common surfactants at the ion head groups through chemical bonds, so that the head group separating force of the surfactants (decamethyibromide) in the ordered aggregation process is suppressed, the surface activity is greatly improved, the action on the biological film of bacteria and viruses is enhanced, and the disinfection capacity of the disinfectant is enhanced. The hyper-branched gemini quaternary ammonium salt is preferably a hydrocarbon single chain with the carbon number of 8-16, and has the strongest disinfection synergistic effect.
In the embodiment of the invention, the hyperbranched chain gemini quaternary ammonium salt is ethylene bis (dodecyl polyoxyethylene/polyoxypropylene block group ammonium bromide), the model is 31627, and the structural formula is
In the embodiment of the invention, the decamethenamine is biquaternary ammonium salt, and the molecule of the decamethenamine is provided with two long-chain hydrophobic groups and two positively charged nitrogen ions, so that the positive charge density on the nitrogen ions is increased through induction, the adsorption of the disinfectant on the surfaces of bacteria and algae cells is facilitated, and the permeability of cell walls is changed, so that the bacteria are broken. In addition, after the disinfectant is adsorbed on the surface of the thalli, hydrophobic groups and hydrophilic groups are favorable to respectively penetrate into a lipoid layer and a protein layer of the thalli cells, so that the enzyme is deactivated and the protein is denatured, and the bisquaternary ammonium salt is hardly influenced by temperature and pH value. The addition of the synergist (hyperbranched chain gemini quaternary ammonium salt) enhances the surface activity of the decamethy bromide, enhances the affinity of the decamethy bromide to a carbon chain, is easier to adsorb bacteria and viruses, and enables the disinfectant to have strong sterilization capability due to the combined effect of the functions.
Glutaraldehyde has good bactericidal effect when the pH value is 3.2-4.6, and the disinfectant has good glutaraldehyde stability in an acidic environment and strong virus inactivating capability when the pH value is about 4.1, and is suitable for room temperature storage.
The embodiment of the invention provides a preparation method of a composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide, which comprises the following steps:
(1) Weighing all raw material components according to the formula, mixing decamethyianium bromide and hyperbranched chain gemini quaternary ammonium salt, and stirring until the mixture is completely dissolved to obtain a first solution;
(2) Mixing glutaraldehyde, ethanol, glycerol and water uniformly to obtain a second solution;
(3) And (3) adding the first solution in the step (1) into the second solution in the step (2), and stirring until the first solution is completely dissolved to obtain the water-soluble organic compound.
Example 1
A composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide comprises the following components in parts by weight per 100g of disinfectant: 5g of glutaraldehyde, 5g of decamethylene bromide, 1g of ethylene bis (dodecyl polyoxyethylene/polyoxypropylene block group ammonium bromide), 15g of glycerol, 5g of ethanol, 3g of polyethylene glycol, 1g of sodium chloride and the balance of deionized water.
The composite veterinary disinfectant containing glutaraldehyde and decamethenamine in the embodiment has stable physical properties, good fluidity and no icing phenomenon at the temperature of-15 ℃.
Example 2
A composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide comprises the following components in parts by weight per 100g of disinfectant: glutaraldehyde 5g, decamethylene bromide 5g, ethylene bis (dodecyl polyoxyethylene/polyoxypropylene block ammonium bromide) 0.5g, and the balance deionized water.
Example 3
A composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide comprises the following components in parts by weight per 100g of disinfectant: glutaraldehyde 5g, decamethylene bromide 5g, and the balance deionized water.
The preparation method of the composite veterinary disinfectant containing glutaraldehyde and decamethenamine in the embodiment is as follows: weighing the raw material components according to the component amounts, dissolving the raw material components in deionized water, uniformly stirring, and fixing the volume to a target volume.
Example 4
A composite veterinary disinfectant containing glutaraldehyde and decamethenamine bromide comprises the following components in parts by weight per 100g of disinfectant: glutaraldehyde 5g, decamethylene bromide 4.5g, ethylene bis (dodecyl polyoxyethylene/polyoxypropylene block group ammonium bromide) 0.5g, glycerol 15g, ethanol 5g, polyethylene glycol 3g, and the balance deionized water.
The composite veterinary disinfectant containing glutaraldehyde and decamethenamine in the embodiment has stable physical properties, good fluidity and no icing phenomenon at the temperature of-20 ℃.
The preparation method of the composite veterinary disinfectant containing glutaraldehyde and decamethenamine in examples 1-2 and 4 comprises the following steps:
(1) Weighing all raw material components according to the formula, mixing decamethyianium bromide and hyperbranched chain gemini quaternary ammonium salt, and stirring until the mixture is completely dissolved to obtain a first solution;
(2) Mixing other components with deionized water and stirring uniformly to obtain a second solution;
(3) Adding the first solution in the step (1) into the second solution in the step (2), adding a proper amount of deionized water for constant volume, and continuously stirring until the deionized water is completely dissolved, thus obtaining the aqueous emulsion.
Application example 1
Examples 1-4 detection of the sterilizing effect of a composite high-efficiency disinfectant containing glutaraldehyde and decamethy bromoammonium:
Referring to the national standard GB15981-1995, the test strain is prepared into a bacterial suspension of 5X 10 6 CFU/mL by PBS, and the test strain is Escherichia coli. Taking the disinfectant of each embodiment, diluting the disinfectant by different multiples (400 times, 800 times, 1600 times, 3200 times and 6400 times) to obtain disinfectant diluents with different concentrations. 2.5mL of the toxin agent diluent is canceled, 2.5mL of bacterial liquid is added and mixed uniformly, 0.05mL is taken after 10 minutes and transferred into 4.95mL of sterilized culture medium, the culture is carried out at the constant temperature of a 37 ℃ incubator, the turbidity of the liquid in the test tube is observed at regular time, and the result is recorded, and the result is shown in Table 1. The sterilizing experimental effects of the disinfectant diluents with different concentrations in examples 1-4 are shown in fig. 1-4, wherein test tube numbers A, B, C, D respectively represent examples 1, 2,3 and 4; subscript 12345 is 1: 400. 1: 800. 1:1600;1:3200;1:6400, for example, reference A 1 in FIG. 1, indicates that the disinfectant of example 1 is 400-fold diluted.
TABLE 1 test results of sterilizing effect
| 1：400 | 1：800 | 1：1600 | 1：3200 | 1：6400 | |
| Example 1 | - | - | - | - | - |
| Example 2 | - | - | - | - | - |
| Example 3 | - | - | - | + | + |
| Example 4 | - | - | - | - | - |
Note that: in Table 1, "-" indicates sterility and "+" indicates sterility.
The sterilization rate results are shown in Table 2.
TABLE 2
From the data in tables 1 and 2, it can be seen that each example exhibited a different antimicrobial effect, with the minimum kill concentration adding at least 4 times the ethylene-based bi-solvent. Among them, the sterilizing effect of examples 2 and 4 was superior to other examples.
Application example 2
Examples 1-4 detection of the content of glutaraldehyde and decamethylene bromide composite high-efficiency disinfectant components:
glutaraldehyde and decamethylene bromide contents of examples 1-4 were measured to meet the standards by reference to the detection method of veterinary drug quality standard (2017 edition).
Application example 3
Referring to GB/T38499-2020 disinfectant stability evaluation method, a constant temperature and humidity box is set to a condition temperature of 54 ℃ according to the regulations, samples of examples 1-4 are put into the box, and the content of each component is measured after 14 days. The glutaraldehyde content of each example after acceleration is shown in Table 3 and the decamethylene bromide content is shown in Table 4.
TABLE 3 glutaraldehyde content of examples 1-4 after acceleration
| Example 1 | Example 2 | Example 3 | Example 4 | |
| Day 0 | 4.98％ | 4.99％ | 4.99％ | 4.99％ |
| Day 7 | 4.87％ | 4.98％ | 4.65％ | 4.99％ |
| Day 14 | 4.75％ | 4.98％ | 4.40％ | 4.97％ |
TABLE 4 Decne methyl bromide content of examples 1-4 after acceleration
| Example 1 | Example 2 | Example 3 | Example 4 | |
| Day 0 | 5.99％ | 5.49％ | 5.00％ | 5.00％ |
| Day 7 | 5.94％ | 5.46％ | 4.95％ | 4.99％ |
| Day 14 | 5.89％ | 5.47％ | 4.92％ | 4.99％ |
Accelerated experiments showed that example 3 had a reduced glutaraldehyde content and that the other examples were stable.
Application example 4
Low temperature stability detection
The disinfectant formulations of examples 1-4 were used for laboratory disinfection experiments, E.coli and Staphylococcus aureus were used as breeding bacteria, porcine reproductive and respiratory syndrome virus was used as viruses, and the disinfection period was 15min.
The disinfection experiment method comprises the following steps:
bacterial sheets (10 5～106 CFU/sheet) infected with specific bacteria (escherichia coli, staphylococcus aureus and porcine reproductive and respiratory syndrome virus) are placed in an autoclave sterilized sterile filter paper bag, and the bag mouth is fastened and placed in different places of a laboratory. After the sterilization is finished, viable bacteria are counted, and the sterilization rate is calculated.
The test bacteria interacted with the disinfectant for a prescribed period of time, the bacterial pieces were placed in a sterile PE tube containing 1.0mL of neutralizing agent, and mixed with an electric mixer for 20s, so that the bacteria on the bacterial pieces were eluted into the neutralizing liquid.
100. Mu.L of the above-mentioned washing solution was aspirated, and the number of viable bacteria was measured by the viable bacteria culture counting method.
Before the sprayer is replaced with disinfectant with new concentration, the disinfectant with new concentration should be cleaned and replaced with the disinfectant with new concentration.
Positive controls were established for viable counts directly with the infectious agent vector.
All test specimens were incubated at 37℃in an incubator and after 24 hours the final count was observed.
The test was repeated 3 times, the number of viable bacteria (CFU/sheet) of each group was calculated, and the sterilization rate (%).
Sterilization rate (Pt) = [ (n 0-nt)/n0 ] ×100% at sterilization t)
Wherein n 0 is the number of viable bacteria in the control group before disinfection; n t is the number of living bacteria in the experimental group after disinfection. The sterilization test results are shown in Table 5.
TABLE 5
The composite veterinary disinfectant sample states of examples 1-4 containing glutaraldehyde and decamethenamine are shown in figure 5 and are examples 1,2, 3 and 4 in sequence from left to right under the condition of freezing and storing at-20 ℃ for 50 days.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (2)
1. The composite veterinary disinfectant containing glutaraldehyde and decamethylene bromide is characterized by comprising the following raw materials in percentage by mass: 1-15% of glutaraldehyde, 1-10% of decamethyiammonium bromide, 0.1-1% of a synergistic agent, 5-20% of ethanol, 6-20% of glycerol and the balance of water;
the synergistic agent is hyperbranched chain gemini quaternary ammonium salt, and the hyperbranched chain gemini quaternary ammonium salt is ethylene bis (dodecyl polyoxyethylene/polyoxypropylene block ammonium bromide).
2. A method for preparing the composite veterinary disinfectant containing glutaraldehyde and decamethyibromoammonium according to claim 1, which is characterized by comprising the following steps:
(1) Weighing the raw material components according to the formula, mixing the decamethyibromoammonium and the synergist, and stirring until the mixture is completely dissolved to obtain a first solution;
(2) Mixing glutaraldehyde, ethanol, glycerol and water uniformly to obtain a second solution;
(3) And (3) adding the first solution in the step (1) into the second solution in the step (2), and stirring until the first solution is completely dissolved to obtain the water-soluble organic compound.
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