CN114916544A - Compound foam type long-acting disinfectant containing citric acid and preparation method and application thereof - Google Patents
Compound foam type long-acting disinfectant containing citric acid and preparation method and application thereof Download PDFInfo
- Publication number
- CN114916544A CN114916544A CN202210502856.3A CN202210502856A CN114916544A CN 114916544 A CN114916544 A CN 114916544A CN 202210502856 A CN202210502856 A CN 202210502856A CN 114916544 A CN114916544 A CN 114916544A
- Authority
- CN
- China
- Prior art keywords
- citric acid
- bromide
- disinfectant
- stirring
- glutaraldehyde
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 title claims abstract description 159
- 239000000645 desinfectant Substances 0.000 title claims abstract description 91
- 239000006260 foam Substances 0.000 title claims abstract description 53
- 150000001875 compounds Chemical class 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229960002233 benzalkonium bromide Drugs 0.000 claims abstract description 27
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims abstract description 27
- VAZJLPXFVQHDFB-UHFFFAOYSA-N 1-(diaminomethylidene)-2-hexylguanidine Polymers CCCCCCN=C(N)N=C(N)N VAZJLPXFVQHDFB-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229920002413 Polyhexanide Polymers 0.000 claims abstract description 23
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 claims abstract description 15
- 241000204031 Mycoplasma Species 0.000 claims abstract description 13
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 10
- 241000700605 Viruses Species 0.000 claims abstract description 9
- 239000003381 stabilizer Substances 0.000 claims abstract description 9
- 241000233866 Fungi Species 0.000 claims abstract description 7
- 239000006184 cosolvent Substances 0.000 claims abstract description 7
- 244000000010 microbial pathogen Species 0.000 claims abstract description 7
- 239000007853 buffer solution Substances 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 5
- 230000000149 penetrating effect Effects 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims description 40
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- -1 polyoxyethylene Polymers 0.000 claims description 15
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 13
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 13
- 239000000194 fatty acid Substances 0.000 claims description 13
- 229930195729 fatty acid Natural products 0.000 claims description 13
- 150000004665 fatty acids Chemical class 0.000 claims description 13
- 229910052757 nitrogen Inorganic materials 0.000 claims description 12
- JDRSMPFHFNXQRB-CMTNHCDUSA-N Decyl beta-D-threo-hexopyranoside Chemical compound CCCCCCCCCCO[C@@H]1O[C@H](CO)C(O)[C@H](O)C1O JDRSMPFHFNXQRB-CMTNHCDUSA-N 0.000 claims description 11
- SWLVFNYSXGMGBS-UHFFFAOYSA-N ammonium bromide Chemical compound [NH4+].[Br-] SWLVFNYSXGMGBS-UHFFFAOYSA-N 0.000 claims description 11
- 229940073499 decyl glucoside Drugs 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 10
- 244000144972 livestock Species 0.000 claims description 9
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims description 8
- 238000009395 breeding Methods 0.000 claims description 8
- 230000001488 breeding effect Effects 0.000 claims description 8
- 235000019864 coconut oil Nutrition 0.000 claims description 7
- 239000003240 coconut oil Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 6
- 244000144977 poultry Species 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 239000005456 alcohol based solvent Substances 0.000 claims description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 2
- BAECOWNUKCLBPZ-HIUWNOOHSA-N Triolein Natural products O([C@H](OCC(=O)CCCCCCC/C=C\CCCCCCCC)COC(=O)CCCCCCC/C=C\CCCCCCCC)C(=O)CCCCCCC/C=C\CCCCCCCC BAECOWNUKCLBPZ-HIUWNOOHSA-N 0.000 claims description 2
- PHYFQTYBJUILEZ-UHFFFAOYSA-N Trioleoylglycerol Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCCCCCCCC)COC(=O)CCCCCCCC=CCCCCCCCC PHYFQTYBJUILEZ-UHFFFAOYSA-N 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 2
- 235000019800 disodium phosphate Nutrition 0.000 claims description 2
- SYELZBGXAIXKHU-UHFFFAOYSA-N dodecyldimethylamine N-oxide Chemical compound CCCCCCCCCCCC[N+](C)(C)[O-] SYELZBGXAIXKHU-UHFFFAOYSA-N 0.000 claims description 2
- 150000003839 salts Chemical group 0.000 claims description 2
- 229940093609 tricaprylin Drugs 0.000 claims description 2
- VLPFTAMPNXLGLX-UHFFFAOYSA-N trioctanoin Chemical compound CCCCCCCC(=O)OCC(OC(=O)CCCCCCC)COC(=O)CCCCCCC VLPFTAMPNXLGLX-UHFFFAOYSA-N 0.000 claims description 2
- PHYFQTYBJUILEZ-IUPFWZBJSA-N triolein Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC PHYFQTYBJUILEZ-IUPFWZBJSA-N 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 49
- 230000001954 sterilising effect Effects 0.000 abstract description 31
- 241001465754 Metazoa Species 0.000 abstract description 25
- 230000002195 synergetic effect Effects 0.000 abstract description 9
- 230000000996 additive effect Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 34
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 24
- 230000000694 effects Effects 0.000 description 20
- 230000002147 killing effect Effects 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 230000002779 inactivation Effects 0.000 description 12
- 241000710777 Classical swine fever virus Species 0.000 description 11
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 11
- 238000012216 screening Methods 0.000 description 9
- 241000711404 Avian avulavirus 1 Species 0.000 description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 description 6
- 206010040880 Skin irritation Diseases 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 231100000475 skin irritation Toxicity 0.000 description 6
- 230000036556 skin irritation Effects 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 230000009471 action Effects 0.000 description 5
- 150000001299 aldehydes Chemical class 0.000 description 5
- 238000004090 dissolution Methods 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 210000004215 spore Anatomy 0.000 description 5
- 244000063299 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 210000004666 bacterial spore Anatomy 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000005187 foaming Methods 0.000 description 4
- 235000013594 poultry meat Nutrition 0.000 description 4
- 239000001509 sodium citrate Substances 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- 241000700159 Rattus Species 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 3
- 231100000460 acute oral toxicity Toxicity 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 210000000554 iris Anatomy 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 description 2
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 2
- 206010010726 Conjunctival oedema Diseases 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 241000283977 Oryctolagus Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 231100000403 acute toxicity Toxicity 0.000 description 2
- 230000007059 acute toxicity Effects 0.000 description 2
- 238000011047 acute toxicity test Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000008233 hard water Substances 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 230000005923 long-lasting effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 231100000286 mucous membrane, eye irritation or corrosion testing Toxicity 0.000 description 2
- XEUYTSFFGRRIIU-UHFFFAOYSA-N n-methyldecan-1-amine;hydrobromide Chemical compound Br.CCCCCCCCCCNC XEUYTSFFGRRIIU-UHFFFAOYSA-N 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 208000028006 Corneal injury Diseases 0.000 description 1
- 206010011026 Corneal lesion Diseases 0.000 description 1
- 206010015946 Eye irritation Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 231100000490 OECD 405 Acute Eye Irritation/Corrosion Toxicity 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010040914 Skin reaction Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 210000000795 conjunctiva Anatomy 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- KAXSXFHMJDMWOM-UHFFFAOYSA-L dipotassium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [K+].[K+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O KAXSXFHMJDMWOM-UHFFFAOYSA-L 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 description 1
- 230000009982 effect on human Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000013 eye irritation Toxicity 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 244000144993 groups of animals Species 0.000 description 1
- 150000002357 guanidines Chemical class 0.000 description 1
- 125000002795 guanidino group Chemical group C(N)(=N)N* 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 230000005571 horizontal transmission Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000009347 mechanical transmission Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 230000009972 noncorrosive effect Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000013613 poultry product Nutrition 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 231100000108 skin corrosion Toxicity 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 230000035483 skin reaction Effects 0.000 description 1
- 231100000430 skin reaction Toxicity 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 235000019614 sour taste Nutrition 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 description 1
- 230000005570 vertical transmission Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/16—Foams
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a compound foam type long-acting disinfectant containing citric acid, and a preparation method and application thereof. The disinfectant comprises the following components in percentage by mass: 0.5-5.0% of citric acid, 0.5-5.0% of benzalkonium bromide, 0.5-5.0% of decamethyl bromide, 0.25-2.5% of glutaraldehyde, 0.5-5.0% of polyhexamethylene biguanide, 3.0-15.0% of cosolvent, 0.1-0.5% of penetrating agent, 0.5-5.0% of foam emulsifier, stabilizer and citric acid can form a buffer system, the pH value is kept to be 3.0-5.0, and the sterilized water is supplemented to 100%. The compounded disinfectant has synergistic or additive effect, kills all pathogenic microorganisms of animal sources including bacteria, viruses, mycoplasma, fungi and the like existing in the covering culture environment, and has the sterilization efficiency of 100 percent.
Description
Technical Field
The invention belongs to the field of biological safety protection articles in livestock and poultry breeding, and particularly relates to a compound foam type long-acting disinfectant containing citric acid, and a preparation method and application thereof.
Background
The serious epidemic diseases of the livestock and poultry seriously damage the development of the breeding industry and threaten the health and public health safety of human beings all the time. Strengthen the prevention and control of animal major epidemic diseases and foreign animal epidemic diseases, and is a great demand for guaranteeing the supply safety of livestock and poultry products and the national biological safety.
The biological safety prevention and control product plays an extremely important role in epidemic disease prevention and control, and the disinfectant is an important component of the biological safety prevention and control product. However, the existing biosafety prevention and control products have some outstanding problems, and the existing products have the problems of low sterilization efficiency, poor safety, poor durability and the like, and lack of breakthrough innovation technology.
The first problem is that: low sterilization efficiency
At present, animal-derived pathogenic microorganisms in a breeding environment comprise bacteria, viruses, mycoplasma, fungi and the like, and often one disinfectant product cannot effectively kill all pathogenic microorganisms, and the commonly used quaternary ammonium salt disinfectant has poor killing effect on spores, mycoplasma and the like of bacteria. Therefore, the problem of low sterilization efficiency is solved by carrying out compound proportioning according to different sterilization spectrums of the disinfectant;
the second problem is that: poor safety
The disinfection of plant is a continuous process, and the product consumption is big, in order to pursue the reduction of cost, often adopt great toxicity disinfection products such as caustic soda (active ingredient is sodium hydroxide), aldehydes (formaldehyde, glutaraldehyde), phenols (phenol), on the one hand has caused the injury to the place animal, also has potential toxic effect to the workman that plant's disinfection used. Many cases of animal death have been reported due to overuse or improper use of disinfectants. The development of a disinfectant which is green, environment-friendly and high in safety is very necessary;
the third problem is that: poor durability
At present, the disinfectant is generally a solution in the using process, and is sprayed on objects such as handrails, cribs or the ground of a livestock house, and due to the high fluidity of the solution, the adhesion and the durability of the disinfectant are poor on irregular surfaces such as handrails and uneven ground, the disinfectant is difficult to fully contact with the objects, the sterilization efficiency is low, and the excessive consumption of the disinfectant is caused by the need of improving the sterilization frequency. This problem makes it possible to develop a disinfecting product with a long-lasting foam, increasing the adhesion and adherence, and giving a long-lasting kill.
Therefore, the development of a green, efficient, safe and low-cost biosafety prevention and control product is urgently needed, the industrial popularization and application are realized, and the green and continuous healthy development of the animal husbandry in China is promoted.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a compound foam type long-acting disinfectant containing citric acid and a preparation method thereof, wherein the disinfectant is environment-friendly, efficient, low in price, free of residues, good in sterilization effect and convenient to transport.
The compound foam type long-acting disinfectant containing citric acid provided by the invention comprises the following components in percentage by mass
Further, the compound foam type long-acting disinfectant containing citric acid comprises the following components in percentage by mass
Preferably, the ratio of the mass of glutaraldehyde to the sum of the masses of benzalkonium bromide and decamethyl bromide may be 1: 2 or more, more preferably 1: (2-4), specifically 1: 2 or 1: 3 or 1: 4;
preferably, the mass ratio of the benzalkonium bromide to the decamethyl bromide can be 1: (1-4), more preferably 1: (1-3), specifically 1: 1 or 1: 2 or 1: 3 or 1: 4;
preferably, the cosolvent can be selected from one or more of alcohol solvents containing 2-10 carbon atoms, such as one or more of alcohol solvents of ethanol, propylene glycol, butanol, isobutanol, octanol and the like;
preferably, the penetrating agent can be selected from one or more of ethanol, glycerol trioleate, tricaprylin, fatty acid polyoxyethylene ether-9, fatty acid polyoxyethylene ether-7, fatty acid polyoxyethylene ether-5 and the like;
preferably, the foam emulsifier can be selected from one or more of dodecyl dimethyl amine oxide, decyl glucoside, coconut oil diethanol amide and hexadecyl trimethyl ammonium bromide; specifically, the foam emulsifier is decyl glucoside, and the mass content of the decyl glucoside is preferably 1%; the foam emulsifier is coconut oil diethanolamide or hexadecyl trimethyl ammonium bromide, and the mass content of the foam emulsifier is preferably 3%.
Preferably, the stabilizer is selected from salts that can form a buffer system with citric acid, such as citrate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium hydrogen phosphate, and the like.
The invention also provides a preparation method of the compound foam type long-acting disinfectant containing citric acid.
The preparation method of the compound foam type long-acting disinfectant containing citric acid provided by the invention comprises the following steps:
(1) introducing nitrogen into part of the sterilized water;
(2) adding the cosolvent into the sterilized water treated in the step (1) and stirring;
(3) adding the decamethyl ammonium bromide and benzalkonium bromide to the system obtained in the step (2) and stirring at a low speed (<150rpm) until complete dissolution;
(4) adding the penetrant and the foam emulsifier into the system obtained in the step (3) and stirring at a low speed (<150rpm) to form a system A;
(5) introducing nitrogen into another part of the sterilized water;
(6) adding the glutaraldehyde and the polyhexamethylene biguanide into the sterilized water treated in the step (5) and stirring at a low speed (<150rpm) until the glutaraldehyde and the polyhexamethylene biguanide are completely dissolved;
(7) adding the citric acid and the stabilizing agent into the system obtained in the step (6), and stirring at a low speed (<150rpm) until the citric acid and the stabilizing agent are completely dissolved; forming a system B;
(8) and transferring the system B into the system A, uniformly stirring at a low speed (<150rpm), metering to 100% by mass with the sterilized water, and keeping the pH value of the system between 3.0 and 5.0.
In the method, the rotation speed of the low-speed stirring is less than 150 rpm.
In the step (4), the stirring time may be 20-60 min.
The compound foam type long-acting disinfectant containing citric acid provided by the invention can effectively kill common pathogenic microorganisms such as bacteria, viruses, mycoplasma, spores, fungi and the like on the ground, railings, food troughs and the like in an animal husbandry environment.
The virus can be hog cholera virus (such as Thiveral hog cholera virus), newcastle disease virus (such as LaSota newcastle disease virus), etc.
The spore is specifically Bacillus subtilis.
The invention also provides a disinfectant composition.
The disinfectant composition protected by the invention comprises citric acid, benzalkonium bromide, decamethyl ammonium bromide, glutaraldehyde and polyhexamethylene biguanide.
Wherein the ratio of the mass of the glutaraldehyde to the sum of the masses of the benzalkonium bromide and the decamethylammonium bromide is 1: 2 and above, preferably 1: (2-4);
the mass ratio of the benzalkonium bromide to the decamethyl bromide is 1: (1-4), preferably 1: (1-3);
the mass ratio of the polyhexamethylene biguanide to the glutaraldehyde is 1: (0.5-5), preferably 1: (1-3);
the mass ratio of the citric acid to the glutaraldehyde is 1: (1-10), preferably 1: (1-8).
Aiming at the problem of low sterilization efficiency of the existing disinfectant. The invention provides a compound formula, which has synergistic effect and can effectively solve the problem of low single-formula sterilization efficiency. The action analysis of each component in the formula is as follows:
1. benzalkonium bromide and decamethyl ammonium bromide are two cationic surfactants, and are enriched on the surface of a cell wall, so that the filtering effect of a microbial cell membrane is disturbed, the permeability of bacterial cells is increased, water permeates into bacteria to cause substance leakage, the metabolism of the bacteria is influenced to destroy the cell structure, and finally the bacteria are dead. The disinfectant has low toxicity and the like, and the disinfectant has high toxicity,
the detergent has strong decontamination capability, is non-corrosive to metal materials, has a synergistic bactericidal effect, has good killing capability to common animal-derived bacteria, but has poor effect on bacterial spores.
2. The killing effect of glutaraldehyde on microbe depends mainly on aldehyde group, and mainly acts on mercapto, hydroxyl, carboxyl and amino of mycoprotein, so that it can make it alkylate to cause protein coagulation to result in bacterial death, and has good killing effect on virus, bacterial spore, fungus and spore and other microbes.
3. Polyhexamethylene biguanide belongs to an environment-friendly disinfectant, guanidino has high activity, and the polymer can be electropositive and can be easily adsorbed by various electronegative bacteria, so that the splitting function of the bacteria and the like is inhibited, and the reproductive capacity of the bacteria and the like is lost.
4. Citric acid, also known as citric acid, having the molecular formula C 6 H 8 O 7 The compound is an important organic acid, is a colorless crystal, is odorless, has strong sour taste, is easy to dissolve in water, can be used as a pH regulator, and has a good killing effect on animal-derived mycoplasma under the condition of low concentration (0.5-1.0%) in the previous experimental study of an inventor.
Test results show that the components of the formula can cooperate with each other to kill all pathogenic microorganisms of animal origin including bacteria, viruses, mycoplasma, fungi and the like existing in the covering culture environment, and the sterilization efficiency is 100%.
Aiming at the problem of poor sterilization safety of the existing disinfectant. In the invention, only glutaraldehyde belongs to aldehyde products and has certain irritation and corrosivity, but due to mutual synergy, the use concentration of glutaraldehyde in the compound is reduced by more than 60-80% compared with that in a single formula, and the toxicity test result shows that the formula product is safe, effective and non-irritating to use.
Aiming at the problem of poor sterilization durability of the existing disinfectant. In the formula of the invention, the SF penetrant containing penetrant, cosolvent, stabilizer and foam forming agent, especially polyhexamethylene biguanide is a high molecular polymer, the product forms a high molecular solution state, and is easy to adsorb various negatively charged bacteria and the like, and rich foam is formed by a foaming gun or a foaming machine when the disinfectant is used, and the foam is attached to the surface of the disinfectant body to play a long-acting and lasting disinfection role.
Compared with the prior art, the invention has the following beneficial effects:
(1) the sterilization range is wide. Because the compounded disinfectant has synergistic or additive effect, the total bactericidal capacity after compounding is enhanced. Quaternary ammonium salts generally cannot kill bacterial spores, and aldehyde disinfectants have good effect on killing bacterial spores. The invention can kill all pathogenic microorganisms of animal origin, including bacteria, viruses, mycoplasma, fungi and the like, existing in the covering and breeding environment by the synergistic action of quaternary ammonium salts, aldehydes, acids and guanidine compounds, and the sterilization efficiency is 100%.
(2) Long-acting and durable. In the formula of the invention, the SF penetrant containing penetrant, cosolvent, stabilizer and foam forming agent, especially polyhexamethylene biguanide is a high molecular polymer, the product forms a high molecular solution state, and is easy to adsorb with various negatively charged bacteria and the like, rich foam is formed by a foaming gun or a foaming machine when the disinfectant is used, and the foam is attached to the surface of a disinfectant body to play a long-acting and lasting disinfection role.
(3) The safety is high. The aldehyde disinfectant has skin irritation and acute toxicity, and in the invention, the dosage of glutaraldehyde is greatly reduced by only 20% of the original dosage through the effective formula synergy, so that the disinfectant has no toxic or side effect on human bodies, and animal toxicological tests show that the formula is efficient, safe and nontoxic.
(4) Can effectively kill mycoplasma. Mycoplasma, as a pathogen, has vertical, horizontal and mechanical transmission capability, and complex and diverse infection routes, and can widely survive in a breeding environment depending on organic biomass. Compared with the prior art, the invention can effectively kill mycoplasma in the culture environment and has important function for effectively controlling the propagation of mycoplasma diseases in the culture farm.
(5) The production process is simple, the stability is good, the expanded production is easy, the on-site disinfection effect is obvious, and the popularization value is very high.
Detailed Description
The present invention will be further illustrated with reference to the following examples, but the present invention is not limited to the following examples. The method is a conventional method unless otherwise specified. The starting materials are commercially available from the open literature unless otherwise specified.
Examples 1,
(1) Synergistic screening of glutaraldehyde and quaternary ammonium salt content
Recovering the glutaraldehyde, the decamethyl ammonium bromide and the benzalkonium bromide to room temperature, wherein the ratio (mass ratio) of the decamethyl ammonium bromide to the benzalkonium bromide is 1: 1, PBS solution is used as a solvent, different disinfectant groups are respectively prepared according to the table 1, bacillus subtilis CMCC (B)63501 is used as an indicator bacterium, a suspension quantitative sterilization test is carried out, and the sterilization rate is used as a screening basis.
TABLE 1 test results for the ratio of glutaraldehyde to quaternary ammonium salt
Note: the above results are the average of 3 trials; the test conditions are all 20 +/-2 ℃; 1, disinfectant: 2 times of dilution, and the action time is 1 h.
The results show that: in group 8, the quaternary ammonium salt is used alone, the spore killing capability is very low (7.8%), the 1% glutaraldehyde compound mass concentration is 0.1% -4% of the quaternary ammonium salt solution, and the sterilization rate is increased along with the increase of the quaternary ammonium salt content. The test result shows that the ratio of the glutaraldehyde to the quaternary ammonium salt is equal to or more than 1: 2 and above, the sterilization rate can reach more than 99.90 percent, and the disinfection effect is qualified.
(2) Synergistic screening of two quaternary ammonium salt contents
The glutaraldehyde, the decamethylammonium bromide and the benzalkonium bromide bulk drugs are returned to room temperature, disinfectant mixed liquor groups for experiments are respectively prepared according to the table 2, and a suspension quantitative sterilization test is carried out by taking bacillus subtilis 63501 as an indicator bacterium. The results were as follows:
TABLE 2 benzalkonium bromide to decamethylammonium bromide ratio test results
Note: the above results are the average of 3 trials; the test conditions are all 20 +/-1 ℃; 1, disinfectant: diluting for 2 times, and acting for 1 h.
The results show that: under the condition that the total content of 1% of glutaraldehyde and 2% of quaternary ammonium salt is constant, the sterilization rate is increased along with the increase of the proportion of decamethyl ammonium bromide. The bactericidal ratio of decamethylammonium bromide to benzalkonium bromide is equal to 1: 1, the bactericidal rate is 99.92%, when being equal to 2: 1, the sterilization rate is 99.98%, and is equal to 3: 1 hour, the sterilization rate is equal to 100 percent, and the disinfection effect is qualified.
(3) Polyhexamethylene biguanide content synergistic screening
The content of polyhexamethylene biguanide is screened, disinfectant mixed liquid groups for experiments are prepared according to the table 3, and a suspension quantitative sterilization test is carried out by taking bacillus subtilis 63501 as an indicator bacterium. The results were as follows:
TABLE 3 polyhexamethylene biguanide formulation test results (%)
The results show that: the content of the polyhexamethylene biguanide is between 0.5 and 1.5 percent, the sterilization rate of each group is more than 99.9 percent, and the disinfection effect is qualified.
(4) Synergistic screening of citric acid content
Screening the citric acid content, respectively preparing disinfectant mixed liquor for experiments according to table 4, and performing suspension quantitative sterilization test by using 10 clinical bovine mycoplasma pathogens (BS1738-BS1748) as indicator bacteria. The results are as follows:
table 4 screening results of citric acid content in System (%)
The results show that: the citric acid content is between 0.5 percent and 4.0 percent, the sterilization rate of each group is more than 99.9 percent, and the disinfection effect is qualified.
(5) Foam emulsifier screening
Screening coconut oil diethanolamide, decyl glucoside and cetyl trimethyl ammonium bromide emulsifier with the content of 1-3% respectively. And (5) testing results. The foam of 1% decyl glucoside is rich and fine, and the foam duration is as long as 60 min. Whereas the 1% coconut oil diethanolamide, 1% cetyltrimethylammonium bromide foam was rare for short duration and at 60min the 1% coconut oil diethanolamide foam completely subsided. 3 percent of coconut oil diethanolamide and 3 percent of hexadecyl trimethyl ammonium bromide can meet the requirement that the foam duration is as long as 60 min.
Example 2 Compound foam type Long-acting disinfectant containing citric acid
The formulation is shown in the following table.
According to the components and the content (w/w), 1.0kg of compound foam type long-acting disinfectant is prepared, and the specific steps are as follows:
(1) introducing nitrogen into part of the sterilized water;
(2) adding propylene glycol to the sterilized water and stirring;
(3) adding the decamethyl bromide and benzalkonium bromide into the system obtained in the step (2) and stirring at a low speed (<150rpm) until complete dissolution;
(4) adding fatty acid polyoxyethylene ether-9 and decyl glucoside into the system obtained in the step (3), and stirring at a low speed (<150rpm) for 20min to form a body system A;
(5) introducing nitrogen into another part of the sterilized water;
(6) adding glutaraldehyde and polyhexamethylene biguanide to the sterilized water and stirring at low speed (<150rpm) to dissolve completely;
(7) adding the citric acid and sodium citrate into the system obtained in the step (6), and stirring at a low speed (<150rpm) until the citric acid and the sodium citrate are completely dissolved; forming a system B;
(8) and transferring the system B into the system A, stirring at a low speed (<150rpm) for 20min, metering the balance by using the sterilized water according to the mass, and measuring the pH of the system to be between 3.0 and 5.0.
Example 3 Compound foam type Long-acting disinfectant containing citric acid
The formulation is shown in the following table.
| Citric acid | 0.5％ |
| Benzalkonium bromide | 1.5％ |
| Decamethylammonium bromide | 3.0％ |
| Glutaraldehyde | 0.5％ |
| Polyhexamethylene biguanide | 1.0％ |
| Propylene glycol + ethanol | 5.0％+5.0％ |
| Fatty acid polyoxyethylene ether-9 | 0.3％ |
| Cetyl trimethyl ammonium Bromide | 3.0％ |
| Disodium hydrogen phosphate-citric acid buffer system | pH of 3.0 to 5.0 |
| Sterilized water | Make up to the rest |
According to the components and the content (w/w), 1.0kg of compound foam type long-acting disinfectant is prepared, and the specific steps are as follows:
(1) introducing nitrogen into part of the sterilized water;
(2) adding propylene glycol and ethanol to the sterilized water and stirring;
(3) adding the decamethyl ammonium bromide and benzalkonium bromide into the system obtained in the step (2) and stirring at a low speed (<150rpm) until complete dissolution;
(4) adding fatty acid polyoxyethylene ether-9 and hexadecyl trimethyl ammonium bromide into the system obtained in the step (3), and stirring at a low speed (<150rpm) for 20min to form a body system A;
(5) introducing nitrogen into another part of the sterilized water;
(6) adding glutaraldehyde and polyhexamethylene biguanide to the sterilized water and stirring at low speed (<150rpm) to dissolve completely;
(7) adding the citric acid and the disodium hydrogen phosphate into the system obtained in the step (6), and stirring at a low speed (<150rpm) until the citric acid and the disodium hydrogen phosphate are completely dissolved; forming a system B;
(8) and transferring the system B into the system A, stirring at a low speed (<150rpm) for 20min, metering the balance by using the sterilized water according to the mass, and measuring the pH of the system to be between 3.0 and 5.0.
Example 4 Compound foam type Long-acting disinfectant containing citric acid
The formulation is shown in the following table.
| Citric acid | 1.0％ |
| Benzalkonium bromide | 1.0％ |
| Decyl methyl ammonium bromide | 2.0％ |
| Glutaraldehyde | 0.5％ |
| Polyhexamethylene biguanide | 1.0％ |
| Propylene glycol | 7.5％ |
| Fatty acid polyoxyethylene ether-9 | 0.25％ |
| Decyl glucoside | 1.0％ |
| Sodium citrate-citric acid buffer system | pH of 3.0 to 5.0 |
| Sterilized water | Make up to the rest |
According to the components and the content (w/w), 1.0kg of compound foam type long-acting disinfectant is prepared, and the specific steps are as follows:
(1) introducing nitrogen into part of the sterilized water;
(2) adding propylene glycol to the sterilized water and stirring;
(3) adding the decamethyl ammonium bromide and benzalkonium bromide into the system obtained in the step (2) and stirring at a low speed (<150rpm) until complete dissolution;
(4) adding fatty acid polyoxyethylene ether-9 and decyl glucoside into the system obtained in the step (3), and stirring at a low speed (<150rpm) for 20min to form a body system A;
(5) introducing nitrogen into another part of the sterilized water;
(6) adding glutaraldehyde and polyhexamethylene biguanide to the sterilized water and stirring at low speed (<150rpm) to dissolve completely;
(7) adding the citric acid and sodium citrate into the system obtained in the step (6), and stirring at a low speed (<150rpm) until the citric acid and the sodium citrate are completely dissolved; forming a system B;
(8) and transferring the system B into the system A, stirring at a low speed (<150rpm) for 20min, metering the volume of the rest sterilized water according to the mass, and measuring the pH value of the system to be 3.0-5.0.
Example 5 Compound foam-type Long-acting disinfectant containing citric acid
The formulation is shown in the following table.
| Citric acid | 1.0％ |
| Benzalkonium bromide | 1.5％ |
| Decyl methyl ammonium bromide | 3.0％ |
| Glutaraldehyde | 0.5％ |
| Polyhexamethylene biguanide | 1.5％ |
| Propylene glycol + ethanol | 5.0％+5.0％ |
| Fatty acid polyoxyethylene ether-7 | 0.25％ |
| Decyl glucoside | 3.0％ |
| Dipotassium hydrogen phosphate-citric acid buffer system | pH of 3.0 to 5.0 |
| Sterilized water | Make up to the rest |
According to the components and the content (w/w), 1.0kg of compound foam type long-acting disinfectant is prepared, and the specific steps are as follows:
(1) introducing nitrogen into part of the sterilized water;
(2) adding propylene glycol and ethanol to the sterilized water and stirring;
(3) adding the decamethyl ammonium bromide and benzalkonium bromide into the system obtained in the step (2) and stirring at a low speed (<150rpm) until complete dissolution;
(4) adding fatty acid polyoxyethylene ether-7 and decyl glucoside into the system obtained in the step (3), and stirring at a low speed (<150rpm) for 20min to form a body system A;
(5) introducing nitrogen into another part of the sterilized water;
(6) adding glutaraldehyde and polyhexamethylene biguanide to the sterilized water and stirring at low speed (<150rpm) to dissolve completely;
(7) adding the citric acid and the dipotassium hydrogen phosphate into the system obtained in the step (6), and stirring at a low speed (<150rpm) until the citric acid and the dipotassium hydrogen phosphate are completely dissolved; forming a system B;
(8) and transferring the system B into the system A, stirring at a low speed (<150rpm) for 20min, metering the volume of the rest sterilized water according to the mass, and measuring the pH value of the system to be 3.0-5.0.
Example 6 Effect of Compound foam type Long-acting disinfectant containing citric acid
(1) Research on hog cholera virus killing test
The inactivation rate of the disinfectant on Thiveral-strain classical swine fever virus is measured by a suspension quantitative method after the disinfectant acts for 3min, 5min, 7min, 10min and 15min respectively under different concentrations. As a result, it was found that 1: the 600 diluted compound long-acting foam disinfectant prepared in the specific embodiment 4 acts on classical swine fever virus Thviral strains for 10min, and the inactivation rate can reach 100%. 1: the 500-diluted compound long-acting foam disinfectant acts on the classical swine fever virus Thiviral strain for 3min, the inactivation rate reaches 70.4%, the acting time is prolonged to 7min, and the inactivation rate of the classical swine fever virus Thiviral strain is improved to 95.2%; when the action time is prolonged to 10min, the inactivation rate of the classical swine fever virus Thiviral strain is improved to 100 percent, and the inactivation effect reaches the standard. When the ratio of 1: when the disinfectant is diluted by 400 percent, the disinfectant only acts on the classical swine fever virus Thiveral strain for 3min, and the inactivation rate can reach 100 percent. Therefore, the compound long-acting foam disinfectant has a good effect of killing the classical swine fever virus T strain and has good virus killing performance.
TABLE 5 inactivation of classical swine fever virus T-strain by disinfectant
Note: the test conditions are all 20 +/-2 ℃, and the results are the average value of 3 tests; the disinfectant diluent here was standard hard water (WS/T798-.
(2) Experimental study on killing of Newcastle disease virus
Suspension killing test is adopted, and chicken embryo infection method is adopted to determine the inactivation rate of the disinfectant on LaSota strain Newcastle disease virus after respectively acting for 5min, 10min and 15min under different concentrations. As a result, it was found that the disinfectant prepared in specific example 4 was diluted up to 1: when 400 hours, the shortest action time is 5min, the inactivation rate of the LaSota strain of the Newcastle disease virus is 100 percent, the LaSota strain acts for 10min and 15min along with the extension of the action time, the inactivation rate is stably kept at 100 percent, and when the inactivation rate is lower than the concentration, the effect is not good. The dilution ratio of the compound long-acting foam disinfectant is 1: has good disinfection effect on the Newcastle disease virus LaSota strain at 400 ℃.
TABLE 6 inactivating effect of disinfectant on Newcastle disease virus
Note: the disinfectant diluent here was standard hard water (WS/T798-.
(3) Research on clinical natural bacteria surface on-site disinfection effect
According to the requirements of 'veterinary disinfectant identification technical specification', more than 4 animal houses of two livestock and poultry farms which do not use any disinfectant within 1 month are selected to be carried out, and the area of the houses is not less than 50m 2 . The device is used for carrying out a field disinfection effect test of the disinfectant.
TABLE 7 grouping of clinical trials
Each experimental colony house was pre-mapped with 3 sampling sites (5X 5 cm) 2 Ground, railing, trough) each site sampled 3 times, the sampling site was adjacent but not the same. Soaking sterilized cotton swab in neutralizer, and placing in a place with area of 5 × 5cm 2 And (3) wiping 10 times horizontally, vertically and forcibly in the area, continuously converting the wiping surface of the swab, cutting the cotton end of the swab into a 10ml sterile centrifugal tube, sealing and marking. Sampling is carried out before disinfection and 30min, 60min and 90min after disinfection respectively. And (3) after the sample is taken back to a laboratory, respectively adding 2ml of sterile normal saline, swirling for 1min, fully eluting as much as possible, then diluting to a proper concentration by using normal saline in a gradient manner, performing bacterial colony counting in an LB culture dish containing 5% calf serum by using a bacterial colony technique, and calculating the sterilization rate.
TABLE 8 killing effect of disinfectant on clinical natural bacteria
The result shows that the sterilization effect of the compound long-acting foam disinfectant prepared in the specific embodiment 4 on the ground, the trough and the railing in the livestock and poultry housing is increased along with the increase of the concentration, and the medium concentration can meet the requirement that the clinical sterilization reaches 90%. Compared with a control drug group, the sterilization is more durable and the effect is more obvious.
The disinfection effect can be realized by other specific embodiments provided by the invention.
(4) Disinfectant safety evaluation test
Safety evaluations of examples were carried out in the GLP laboratory, including oral acute toxicity tests in rats, acute skin irritation tests in rabbits, and acute eye irritation tests in rabbits.
SD rats were used to evaluate the acute oral toxicity of the disinfectant in the examples, according to the guidelines for acute toxicity (LD50 assay) in veterinary medicine. 10 SD rats (female and male halves) were orally and gastronasally infected with 1009mg/kg b.w., 1453mg/kg b.w., 2093mg/kg b.w., 3014mg/kg b.w., 4340mg/kg b.w., 6250mg/kg b.w., and 9000mg/kg b.w., respectively. Body weights were taken on day of exposure (day 0), day 7 and day 14. Detailed symptom observations were made before and about 20 minutes, 2 hours, and 4 hours after the infection on day 0, and once a day from day 1 to day 14. All infected animals were dissected gross.
Referring to the Disinfection Specification (2002 edition), Rabbit acute skin irritation/corrosivity test was carried out on disinfectant using Japanese white rabbit as test animal. 3 male rabbits were used for the test, and 0.5mL of the test sample stock solution was evenly spread on the right skin of the back of 3 animals per day, the left skin was used as a control, the test sample was not added, and the exposure time was 4 h. The application is carried out once a day for 14 d. Clinical observations were made once daily 1 day before and 1 day after each application for 24 h. Skin reactions at the sites of administration of the test samples were observed 24h after each day of administration and scored according to skin irritation response scoring criteria.
Referring to the Disinfection technical Specification (2002 edition), Japanese white rabbits were used as test animals to perform rabbit acute eye irritation/corrosion tests on disinfectant. 3 male rabbits were used for the test, each animal was given 0.1mL of test sample in the right eye and control in the left eye. Clinical observation is carried out once a day after infection. Eye examination was performed at 1h, 24h, 48h, 72h post-exposure, and the "mean score" for each animal was calculated for each animal in terms of corneal damage, iris damage, conjunctival hyperemia, and conjunctival edema at different observation times (24h, 48h, 72h) (i.e., the sum of the scores for 24h, 48h, 72h for each animal divided by the number of observations 3). The stimulation intensity of the test sample to the eyes is judged according to the grading standard of the eye stimulation response by respectively carrying out the average scores and the recovery time of the cornea, iris and conjunctiva congestion and edema of the eyes of the animals.
The results were as follows:
TABLE 9 disinfectant safety evaluation test results
Note: LD (laser diode) 50 Is half-lethal, in units of: mg/kg b.w.
In oral acute toxicity tests, no death was observed in all of the first to fifth groups of animals in the examples, some of the examples began with death in the sixth group (6250mg/kg b.w.), and the half-fatality rate of the disinfectant in examples 2-5 was greater than 5000mg/kg b.w. for both female and male mice, calculated based on the 95% confidence interval, indicating that the disinfectant was non-toxic.
In skin irritation, all test animals showed no skin damage after 14 consecutive days of exposure, indicating no skin irritation.
In the eye irritation test, after 24h, 48h and 72h of infection, corneal lesion, iris lesion, conjunctival congestion and conjunctival edema of the right eye of all test animals are not seen, which indicates that no eye irritation exists.
Claims (10)
1. A compound foam type long-acting disinfectant containing citric acid comprises the following components in percentage by mass:
2. the compound foam type long-acting disinfectant containing citric acid according to claim 1, wherein: the compound foam type long-acting disinfectant containing citric acid comprises the following components in percentage by mass:
3. the compound foam type long-acting disinfectant containing citric acid according to claim 1 or 2, wherein:
the ratio of the mass of the glutaraldehyde to the sum of the masses of the benzalkonium bromide and the decamethylammonium bromide is 1: 2 or more, preferably 1: (2-4);
the mass ratio of the benzalkonium bromide to the decamethyl bromide is 1: (1-4), preferably 1: (1-3);
the cosolvent is selected from one or more of alcohol solvents containing 2-10 carbon atoms;
the penetrating agent is selected from one or more of ethanol, glycerol trioleate, tricaprylin, fatty acid polyoxyethylene ether-9, fatty acid polyoxyethylene ether-7 and fatty acid polyoxyethylene ether-5;
the foam emulsifier is selected from one or a mixture of dodecyl dimethyl amine oxide, decyl glucoside, coconut oil diethanolamide and hexadecyl trimethyl ammonium bromide;
the stabilizer is selected from salts capable of forming a buffer system with citric acid, including citrate, disodium hydrogen phosphate, dipotassium hydrogen phosphate or sodium hydrogen phosphate.
4. A compound foam type long-acting disinfectant containing citric acid according to any one of claims 1-3, wherein: the compound foam type long-acting disinfectant containing citric acid can kill pathogenic microorganisms including bacteria, viruses, mycoplasma, spores and fungi in the livestock breeding environment.
5. A method for preparing a long-acting disinfectant of the citric acid-containing compound foam type according to any one of claims 1 to 4, comprising the steps of:
(1) introducing nitrogen into part of the sterilized water;
(2) adding the cosolvent into the sterilized water treated in the step (1) and stirring;
(3) adding the decamethyl ammonium bromide and the benzalkonium bromide into the system obtained in the step (2) and stirring at a low speed until the decamethyl ammonium bromide and the benzalkonium bromide are completely dissolved;
(4) adding the penetrant and the foam emulsifier into the system obtained in the step (3) and stirring at a low speed to form a system A;
(5) introducing nitrogen into another part of the sterilized water;
(6) adding the glutaraldehyde and the polyhexamethylene biguanide into the sterilized water treated in the step (5) and stirring at a low speed until the glutaraldehyde and the polyhexamethylene biguanide are completely dissolved;
(7) adding the citric acid and the stabilizing agent into the system obtained in the step (6), and stirring at a low speed (<150rpm) until the citric acid and the stabilizing agent are completely dissolved; forming a system B;
(8) and transferring the system B into the system A, uniformly stirring at a low speed, metering the volume to 100% by mass by using the sterilized water, and keeping the pH value of the system between 3.0 and 5.0.
6. The method of claim 5, wherein: the rotating speed of the low-speed stirring is less than 150 rpm; in the step (4), the stirring time is 20-60 min.
7. The use of a long-acting disinfectant of the citric acid-containing syntactic foam type according to any one of claims 1 to 4, characterized in that: the compound foam type long-acting disinfectant containing the citric acid is diluted by 100-fold and 200-fold with water and is used for disinfecting the environment or objects.
8. Use according to claim 7, characterized in that: the environment is a livestock and poultry breeding environment, and the object is an object in the livestock and poultry breeding environment.
9. A disinfectant composition comprises citric acid, benzalkonium bromide, decamethylammonium bromide, glutaraldehyde and polyhexamethylene biguanide.
10. The disinfectant composition according to claim 9, wherein: the ratio of the mass of the glutaraldehyde to the sum of the masses of the benzalkonium bromide and the decamethylammonium bromide is 1: 2 or more, preferably 1: (2-4);
the mass ratio of the benzalkonium bromide to the decamethyl bromide is 1: (1-4), preferably 1: (1-3);
the mass ratio of the polyhexamethylene biguanide to the glutaraldehyde is 1: (0.5-5), preferably 1: (1-3);
the mass ratio of the citric acid to the glutaraldehyde is 1: (1-10), preferably 1: (1-8).
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| CN202210502856.3A CN114916544A (en) | 2022-05-10 | 2022-05-10 | Compound foam type long-acting disinfectant containing citric acid and preparation method and application thereof |
| NL2032962A NL2032962A (en) | 2022-05-10 | 2022-09-06 | Foam type long-acting citric acid-containing compound disinfectant, preparation method and use thereof |
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| CN202210502856.3A CN114916544A (en) | 2022-05-10 | 2022-05-10 | Compound foam type long-acting disinfectant containing citric acid and preparation method and application thereof |
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| CN115530166A (en) * | 2022-10-12 | 2022-12-30 | 湖南湘农动物药业有限公司 | Composite veterinary disinfectant containing glutaraldehyde and ammonium decamethylammonium bromide and preparation method thereof |
| CN115804375A (en) * | 2022-11-28 | 2023-03-17 | 郑州百瑞动物药业有限公司 | Foam disinfectant for farm and preparation method thereof |
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| CN115804375A (en) * | 2022-11-28 | 2023-03-17 | 郑州百瑞动物药业有限公司 | Foam disinfectant for farm and preparation method thereof |
| CN115804375B (en) * | 2022-11-28 | 2024-06-11 | 郑州百瑞动物药业有限公司 | Foam disinfectant for farms and preparation method thereof |
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| NL2032962A (en) | 2023-11-16 |
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