CN101518216B - Method for seed production of O.mossambicus and O.hornorum - Google Patents
Method for seed production of O.mossambicus and O.hornorum Download PDFInfo
- Publication number
- CN101518216B CN101518216B CN200910038389A CN200910038389A CN101518216B CN 101518216 B CN101518216 B CN 101518216B CN 200910038389 A CN200910038389 A CN 200910038389A CN 200910038389 A CN200910038389 A CN 200910038389A CN 101518216 B CN101518216 B CN 101518216B
- Authority
- CN
- China
- Prior art keywords
- tilapia mossambica
- male
- parent
- lotus
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a method for seed production of O.mossambicus and O.hornorum, and belongs to the field of breeding fish. The method discloses that the O.mossambicus is taken as a female parent, and the heredity similarity coefficient between individuals in a colony is more than 90.6 percent; the O.hornorum is taken as a male parent, and the heredity similarity coefficient between individuals in a colony is more than 91.2 percent; the parent fish of the female parent and male parent is bred for five years; and the interspecific hybridization of the female and male parent fish is performed according to the ratio of 3:1 to generate the O.mossambicus and O.hornorum, of which the male ratio of the tilapia F1 is more than 95 percent. The method has the advantages of realizing the singlemale breeding, solving the problem of excessive reproduction in the breeding of the tilapia, simultaneously speeding up the growth of the tilapia, improving the colony output and lowering production cost.
Description
Technical field:
The present invention relates to the method for fish breeding, the breeding method of Tilapia mossambica interspecific cross specifically.
Technical background:
Tilapia mossambica (tilapia) is one of important kind of freshwater aquaculture industry, and 85 countries and regions cultivating tilapias are arranged in the world.China is maximum in the world Tilapia mossambica producing country, increase of production during "the 10th five-years" 65.3%, increase by 10.5%, 2008 annual production every year and surpass 1,200,000 tons, account for 50% of Gross World Product.Tilapia mossambica also is large exported product in China's freshwater aquiculture kind, and 2007 annual export volumes and export amount are respectively 21.5 ten thousand tons and 4.9 hundred million dollars.
Though China Tilapia mossambica has been obtained the success of introducing a fine variety breed, but still there are many problems.It at first is the germplasm problem during seed produces; Become the bottleneck of restriction industry development: because Tilapia mossambica is external introduced variety; Introduce the difference in kind, area, it is impure to cause existing kind germplasm to mix, its seed at growth rate, male ratio, rise and catch rate, cold resistance, feed conversion rate etc. and culture aspect the key elements uneven; Culture efficiency is relatively poor, has influenced industrialized development.
Because the long speed of tilapia male sashimi (raw fish) is faster about 30% than raun, can accelerate growth rate and directly improve pond yields, reduces production costs.Therefore, culture complete male Tilapia mossambica and not only can solve its excessive multiplication problem but also can tachyauxesis, improve population yield.Occurring in nature, there are two kinds of dissimilar sex chromosome (ZW ♀-ZZ ♂ and XX ♀-XY ♂) in Tilapia mossambica, therefore, carries out the Tilapia mossambica interspecific cross and can produce complete male Tilapia mossambica seed, and this also is the main method and the trend of producing complete male Tilapia mossambica seed at present.The Tilapia mossambica kind of culturing at present mainly is a Buddhist nun fish difficult to understand (bolti * Oreochromis aureus).But body colour of Buddhist nun fish difficult to understand and phenotype are similar with its female parent, and naked eyes are difficult to hybrid and female parent are told.Therefore cause easily mixing between crossbreed and parent, thereby reduce the quality (male ratio, growth rate etc.) of seed.
Orange Mozambique Tilapia mossambica (O.mossambicus ♀) * He Nalong Tilapia mossambica (O.hornorum ♂) hybrid generation male ratio is high, and growth is fast, and body colour and parent distinguish obviously, for the guarantor's kind and the production high-quality seed of production of hybrid seeds unit offers convenience.
Summary of the invention:
The object of the invention provides the producing method for seed of a kind of not lotus Tilapia mossambica; Utilize the method for Tilapia mossambica interspecific cross to obtain high male first filial generation (crossbreed); Carry out the single male breed of Tilapia mossambica, solved excessive multiplication problem in the Tilapia mossambica breed, can accelerate the growth rate of Tilapia mossambica simultaneously; Improve population yield, reduce production costs.
Technical scheme of the present invention is; With orange Mozambique Tilapia mossambica (O.mossambicus) is female parent, and the genetic similarity in its colony between the individuality is more than 90.6%, and He Nalong Tilapia mossambica (O.hornorum) is a male parent; Genetic similarity in its colony between the individuality is more than 91.2%; The parent population of said female parent, male parent is all through seed selection in 5 years, and the male and female parent population carries out interspecific cross with three examples that make a comparison, and the male ratio of producing the Tilapia mossambica hybrid generation is the not lotus Tilapia mossambica more than 95%.
Intragroup genetic resemblance degree is that by formula S=2Nxy/ (Nx+Ny) calculates genetic resemblance degree between individuality (S is the genetic resemblance degree in the formula; Nx and Ny are respectively the amplified band number of individual x and y; Nxy is the total band number of individual x and y), intragroup genetic resemblance degree is through the similarity factor simple average between two all in colony individuals is tried to achieve.
The above male and female parent population is through seed selection, and body weight all is stabilized in more than 400 grams, and physique is healthy and strong, and gonad development is good.
The maternal parent population of orange Mozambique according to the invention Tilapia mossambica must possess the condition that is no less than with the next item down:
1. body colour is bright-coloured, is orange-yellow;
2. body length is 2.56 ± 0.08 with height ratio;
3. body length is 3.61 ± 0.09 with head length ratio;
4. caudal peduncle length is 0.72 ± 0.04 with the caudal peduncle ratios;
5. left side mandibular arch outside gill raker number is 19～27;
6. right side mandibular arch outside gill raker number is 19～26;
7. dorsal fin is X V～X VII11～13;
8. anal fin is III9～11;
9. going up lateral line scales is 18～25;
10. descending lateral line scales is 10～15;
More than He Nalong Tilapia mossambica male parent parent population according to the invention must possess the condition that is no less than with the next item down:
1. body colour cinerous;
2. body length is 1.89 ± 0.10 with height ratio;
3. body length is 3.45 ± 0.09 with head length ratio;
4. caudal peduncle length is 0.41 ± 0.05 with the caudal peduncle ratios;
5. left side mandibular arch outside gill raker number is 18～27;
6. right side mandibular arch outside gill raker number is 20～28;
7. dorsal fin is X III～X V9～12;
8. anal fin is III9～11;
9. going up lateral line scales is 14～20;
10. descending lateral line scales is 7～13;
The terminal color of the not lotus Tilapia mossambica pectoral fin of hybrid generation according to the invention and abdomeinal fin is red; Pectoral fin of the parent population of said female parent, male parent and abdomeinal fin end are orange or cinerous; Difference obviously; Avoided the germplasm of Tilapia mossambica to mix, for the parent of production of hybrid seeds unit management and production high-quality seed offer convenience.
The above not the lotus Tilapia mossambica can in fresh water, culture, or in the seawater breeding of 18 ‰ salinity.
Female parent of orange Mozambique of the present invention Tilapia mossambica and the male parent of He Nalong Tilapia mossambica will guarantee that all purity is high, and through seed selection in 5 years, physique was healthy and strong, gonad development is good, hybridized, and can obtain the high male ratio of its first filial generation.
Advantage of the present invention is to utilize the method for maternal parent population of orange Mozambique Tilapia mossambica and the interspecific cross of He Nalong Tilapia mossambica male parent parent population to obtain high male first filial generation; Carry out the single male breed of Tilapia mossambica; Solve excessive multiplication problem in the Tilapia mossambica breed; Simultaneously can accelerate the growth rate of Tilapia mossambica, improve population yield, reduce production costs.
Embodiment:
1, creates the suitable artificial ecoenvironment of fish.
2, be female parent with orange Mozambique Tilapia mossambica; Genetic similarity in its colony between the individuality is more than 90.6%, and the He Nalong Tilapia mossambica is a male parent, and the genetic similarity in its colony between the individuality is more than 91.2%; Two kinds of parent populations of said male and female are all through seed selection in 5 years; Physique is healthy and strong, and gonad development is good, and body weight is stable at more than 400 grams.
3, the scalability shape of orange Mozambique Tilapia mossambica and He Nalong Tilapia mossambica
4, the denumerable proterties of orange Mozambique Tilapia mossambica and He Nalong Tilapia mossambica
| The He Nalong Tilapia mossambica | Orange Mozambique Tilapia mossambica | |
| Left side first cheek | 18～27 | 19～27 |
| Bow outside cheek rake | (24.00±1.40) | (24.00±1.40) |
| Left side first cheek | 24～30 | 23～31 |
| Bend inboard cheek rake | (27.63±1.01) | (27.13±1.63) |
| Right side first cheek | 20～28 | 19～26 |
| Bow outside cheek rake | (23.48±1.25) | (22.23±1.18) |
| Right side first cheek | 24～30 | 23～30 |
| Bend inboard cheek rake | (27.73±1.44) | (26.93±1.24) |
| Last lateral line scales | 14～20(17.45±1.05) | 18～25(21.70±1.65) |
| Following lateral line scales | 7～13(10.00±1.25) | 10～15(13.45±0.95) |
| The hard sour jujube of dorsal fin | XIII～XV(13～15) | XV～XVII(15～17) |
| The soft sour jujube of dorsal fin | 9～12(10.93±0.52) | 11～13(11.73±0.47) |
| The pectoral fin fin ray | 12～13 | 11～13 |
| The abdomeinal fin fin ray | I+5 | I+5 |
| The hard sour jujube of anal fin | III9～11 | III9～11 |
| Soft of anal fin | (9.53±0.55) | (10.1±0.36) |
5, the maturation age of two parent Tilapia mossambicas
Orange Mozambique Tilapia mossambica and He Nalong Tilapia mossambica all belong to repeatedly and lay eggs, and the minimum body length of two parents is respectively 6.60cm and 8.64cm gets final product sexual maturity.It is female respectively to dissect 10 tail parent Tilapia mossambicas in the test, calculates its mature egg number, fish brood amount, fertility relatively.
Maturity of fish gonads=100 * sexual gland weigh/goes the body weight behind the internal organ
The ovum grain number * ovary gross weight/n of absolute fertility (fish brood amount)=n gram sample
Relative fertility=fish brood amount/fish body weight (gram).
The result is like table
Two parents' gonad maturity index
| Body long (cm) | Absolute fertility (grain) | Mature coefficient | Relative fertility | |
| Orange Mozambique Tilapia mossambica | 6.60～8.91 | 620～971(702 ±183) | 3.566±1.606 | 31.233±7.190 |
| The He Nalong Tilapia mossambica | 8.64～10.94 | 716～983(810 ±156) | 1.464±0.718 | 17.860±3.191 |
6, the production of hybrid seeds
Put 15d before the parent population in a suitable place to breed, reply is bred the pond and is leveled sterilization, and the pond water temperature is gone up and when being stabilized in more than 18 ℃, can be put parent population in a suitable place to breed.The male and female parent population is put in a suitable place to breed in 3: 1 ratios.
After parent population is put into and breeds the pond, should strengthen feeding and management, keep pond water quality pure and fresh, dissolved oxygen is sufficient, and the new water of in good time annotating keeps suitable water temperature, stimulates parent population to oestrus and lays eggs.When water temperature stability more than 23 ℃ the time, pool 10d～15d under the parent population, parent population promptly oestruses and chases, and digs the nest mating; After 7d～10d, visible pool side has fry to move about in groups, meets scaring promptly by (being epimeletic behavior) in the dam fish suction inlet, numerous seedling success this moment.
7, emerge
Self-operation is lived, the group time that looses as yet when fry goes, and parent population begins to stop epimeletic behavior, just can begin to drag for seedling.After seeing pool side the crowd's that looses fry being arranged, the diddle-net that adopts bolting silk to process is fished for before sunrise every day, with seeing with dragging for; Or fish for once with the full pond of the every 10d of close net.Fry is dragged for to breeding spand and cultivates.Concentrate a collection ofly, cultivate a collection ofly, make the synchronous cultivation same period of fry of same size, avoid killing and devouring mutually because of individual difference.Reach more than 95% through statistics fry male ratio.
Orange Mozambique Tilapia mossambica female parent obviously can make a distinction with the hybridization first filial generation that He Nalong Tilapia mossambica male parent is hybridized production in appearance; Pectoral fin of two kinds of parent populations of said male and female and abdomeinal fin end are orange or cinerous; And the terminal color of the pectoral fin of hybridization first filial generation and abdomeinal fin is red; Because difference obviously, avoided the germplasm of Tilapia mossambica to mix, for the germplasm protection and the production high-quality seed of production of hybrid seeds unit offers convenience.
The not lotus Tilapia mossambica of the production of hybrid seeds of the present invention can be cultured in fresh water, also can be in the seawater breeding of 18 ‰ salinity.
Claims (3)
1. the producing method for seed of lotus Tilapia mossambica not is characterized in that with orange Mozambique Tilapia mossambica be female parent, and body colour is bright-coloured; Be orange-yellow, the genetic similarity in its colony between the individuality is more than 90.6%, and body length is 2.56 ± 0.08 with height ratio; Body length is 3.61 ± 0.09 with head length ratio, and caudal peduncle length is 0.72 ± 0.04 with the caudal peduncle ratios, and the He Nalong Tilapia mossambica is a male parent; Body colour cinerous, the genetic similarity in its colony between the individuality are more than 91.2%, and body length is 1.89 ± 0.10 with height ratio; Body length is 3.45 ± 0.09 with head length ratio, and caudal peduncle length is 0.41 ± 0.05 with the caudal peduncle ratios, and the parent population of said female parent, male parent is all through seed selection in 5 years; The male and female parent population carries out interspecific cross with three examples that make a comparison; The male ratio of producing Tilapia mossambica hybridization first filial generation is the not lotus Tilapia mossambica more than 95%, and the terminal color of lotus Tilapia mossambica pectoral fin and abdomeinal fin is not red, and pectoral fin of the parent population of said female parent, male parent and abdomeinal fin end are orange or black.
2. the producing method for seed of a kind of not lotus Tilapia mossambica according to claim 1 is characterized in that said male and female parent population through seed selection, and body weight all is stabilized in more than 400 grams, and physique is healthy and strong, and gonad development is good.
3. the producing method for seed of a kind of not lotus Tilapia mossambica according to claim 1 is characterized in that the lotus Tilapia mossambica can not cultured in fresh water, or the seawater breeding of 18 ‰ salinity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910038389A CN101518216B (en) | 2009-04-03 | 2009-04-03 | Method for seed production of O.mossambicus and O.hornorum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910038389A CN101518216B (en) | 2009-04-03 | 2009-04-03 | Method for seed production of O.mossambicus and O.hornorum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101518216A CN101518216A (en) | 2009-09-02 |
| CN101518216B true CN101518216B (en) | 2012-09-05 |
Family
ID=41079180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910038389A Expired - Fee Related CN101518216B (en) | 2009-04-03 | 2009-04-03 | Method for seed production of O.mossambicus and O.hornorum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101518216B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070095A (en) * | 2012-11-29 | 2013-05-01 | 中国水产科学研究院淡水渔业研究中心 | Fine breed preservation method of genetically improved farmed tilapia form selection |
| CN104542397B (en) * | 2014-12-31 | 2019-04-05 | 茂名市伟业罗非鱼良种场 | A kind of polynary parents producing method for seed of Tilapia mossambica |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435090A (en) * | 2002-01-30 | 2003-08-13 | 李思发 | Fish breeding technique |
| CN101292633A (en) * | 2007-04-28 | 2008-10-29 | 李思发 | Seedling production method for hybridization fish-Ji'ao tilapia |
-
2009
- 2009-04-03 CN CN200910038389A patent/CN101518216B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435090A (en) * | 2002-01-30 | 2003-08-13 | 李思发 | Fish breeding technique |
| CN101292633A (en) * | 2007-04-28 | 2008-10-29 | 李思发 | Seedling production method for hybridization fish-Ji'ao tilapia |
Non-Patent Citations (4)
| Title |
|---|
| 朱华平等.橙色莫桑比克罗非鱼(O reochromis mossambicus)和荷那龙罗非鱼(O.hornorum)的选育效果评价.《南方水产》.2008,第4卷(第3期),第2页左栏第2段,第2.4,3节. * |
| 杨淞等.5种杂交F1罗非鱼生长性能比较研究.《淡水渔业》.2006,第36卷(第4期),41-44. * |
| 杨淞等.两种罗非鱼主要生物学性状及杂交F1代生长性能的研究.《四川农业大学学报》.2008,第26卷(第1期),第1.1,1.2.5,2.1,2.4,2.6,3节、表2-3,7. * |
| 杨淞等.莫桑比克罗非鱼和荷那龙罗非鱼耐盐性的初步研究.《齐鲁渔业》.2006,第23卷(第5期),7-10. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101518216A (en) | 2009-09-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102318571B (en) | Culture method of hybrid new variety of Argopecten irradias and Argopecten purpuratus | |
| CN103120134B (en) | Pollution-free freshwater shrimp cultivation method | |
| CN102696520B (en) | Method for producing seeds of new Nero-Aurea tilapia | |
| WO2015021785A1 (en) | Method for breeding new-variety crassostrea gigas with orange left and right shells | |
| CN105248320A (en) | Freshwater shrimp culture method | |
| CN103814864B (en) | A kind of green-egg-shelled duck selection | |
| CN108739656A (en) | A kind of quaternary lean meat pig breeding method | |
| CN104686423A (en) | Colony-family-molecule comprehensive genetic breeding method for takifugu obscurus | |
| CN110024722A (en) | A kind of artificial fecundation method of scavenger | |
| CN101658146A (en) | Salt tolerant breeding technology for tilapia | |
| CN1444848A (en) | Industrial breeding method for bevel-band rockfish | |
| AU2021104593A4 (en) | Cultivation method of first-generation commercial seed hybrids of female Patinopecten caurinus and male Patinopecten yessoensis | |
| CN102860273B (en) | Crossbreeding method of mud crab | |
| CN107581115B (en) | Method for improving survival rate of tridacna from juvenile stage to juvenile stage | |
| CN101518216B (en) | Method for seed production of O.mossambicus and O.hornorum | |
| CN101461336B (en) | Method for establishing and breeding multiresistance fish population, variety (strain) and family | |
| CN109804952B (en) | Artificial breeding method for decapterus maruadsi | |
| CN103918583A (en) | Pearl mussel breeding method | |
| CN114375912B (en) | Large-scale breeding method for all-male freshwater shrimps | |
| CN114403068B (en) | Breeding method of low-salt-tolerance palaemon carinicauda strain | |
| CN104273073A (en) | Breeding method for bay purple hybrid golden yellow adductor muscle scallops | |
| CN100546466C (en) | A kind of hydriopsis cumingii and Chidie clam distant hybrid breeding method | |
| CN108522378A (en) | The breeding method of female Alaska scallop and male Yessoensis generation commodity offspring seed | |
| CN101103713A (en) | Plecoglossus altivelis artificial reproduction method | |
| CN101292633A (en) | Seedling production method for hybridization fish-Ji'ao tilapia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120905 Termination date: 20140403 |