CN104542397B - A kind of polynary parents producing method for seed of Tilapia mossambica - Google Patents
A kind of polynary parents producing method for seed of Tilapia mossambica Download PDFInfo
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- CN104542397B CN104542397B CN201410854647.0A CN201410854647A CN104542397B CN 104542397 B CN104542397 B CN 104542397B CN 201410854647 A CN201410854647 A CN 201410854647A CN 104542397 B CN104542397 B CN 104542397B
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- 241000276701 Oreochromis mossambicus Species 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000219233 Oreochromis aureus Species 0.000 claims abstract description 50
- 238000009396 hybridization Methods 0.000 claims abstract description 30
- 230000001488 breeding effect Effects 0.000 claims abstract description 23
- 238000009395 breeding Methods 0.000 claims abstract description 21
- 238000009403 interspecific hybridization Methods 0.000 claims abstract description 15
- 241000251468 Actinopterygii Species 0.000 abstract description 19
- 238000004519 manufacturing process Methods 0.000 abstract description 15
- 230000008901 benefit Effects 0.000 abstract description 8
- 238000010899 nucleation Methods 0.000 abstract description 2
- 241000276707 Tilapia Species 0.000 description 13
- 230000008774 maternal effect Effects 0.000 description 13
- 125000000058 cyclopentadienyl group Chemical group C1(=CC=CC1)* 0.000 description 12
- ZSWFCLXCOIISFI-UHFFFAOYSA-N endo-cyclopentadiene Natural products C1C=CC=C1 ZSWFCLXCOIISFI-UHFFFAOYSA-N 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 241000252233 Cyprinus carpio Species 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 239000004568 cement Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241001275890 Megalobrama amblycephala Species 0.000 description 2
- 108091092878 Microsatellite Proteins 0.000 description 2
- 241000277275 Oncorhynchus mykiss Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000219823 Medicago Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241000276719 Oreochromis Species 0.000 description 1
- 241000276703 Oreochromis niloticus Species 0.000 description 1
- 241000276677 Sarotherodon Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009343 monoculture Methods 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of producing method for seed of Tilapia mossambica, the producing method for seed breeds to obtain first filial generation bolti the following steps are included: Xin Jifu strain bolti and the Sudan's strain bolti are first carried out intraspecific hybridization；It then is female parent with the raun in obtained first filial generation bolti, the milter in Oreochromis aureus is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.The seeding technique combined using Tilapia mossambica intraspecific hybridization and interspecific hybridization is gathered together various merits, improves the output of seedling and male rate of offspring of Tilapia mossambica, to improve the yield and benefit of Rofe fish culture；The present invention also provides Tilapia mossambica obtained by adopting the seed production method, which has many advantages, such as that output of seedling height and male rate of offspring are high.
Description
Technical field
The present invention relates to a kind of producing method for seed of Tilapia mossambica, and in particular to a kind of producing method for seed of multiple hybrid tilapia.
Background technique
Tilapia mossambica is one of the principal item of China's freshwater aquiculture, is also important aquatic products for export and (sends out referring to Li Si
The development prospect and bottleneck problem [J] science of China's Tilapia mossambica industry are breeded fish, 2003,12 (9): 3-5.).Since female, milter is raw
There are significant difference between length, monoculture (male) has obvious economic benefit.Interspecific hybridization is Tilapia mossambica scale unisexuality nursery
Most safe, practical biological method in production, a large number of experiments show that, bolti Oreochromis niloticus ♀
× Oreochromis aureus O.aureusThe heterosis, hybrid vigor that cross combination obtains is the most obvious, and not only male ratio is high for first-filial generation,
And the speed of growth is fast (referring to Li Sifa, Chen Lin, Cai Wanqi tilapia (JeFu luoluo fish ♀ × Oreochromis aureus) and 4 nearly edge genotype Tilapia mossambicas hereditary difference RAPD, SSR comparative analysis [J] aquatic product journal, 2008,32 (5):
657-664.Xiao Wei, Li great Yu, Yang Hong wait tetra- kinds of cross combination Sarotherodon sps of and its growth comparative study [J] of parent
Aquatile journal, 2012,36 (5): 905-912.).But in actual production, bolti ♀ × Oreochromis aureus
The phenomenon that there is also output of seedling is relatively low for hybridization, male ratio is unstable, affect to a certain extent Buddhist nun Austria heterosis, hybrid vigor utilize with it is miscellaneous
Kind is promoted.
Hybrid vigour is generally dependent on amphilepsis purity height with Relative Hereditary difference size (referring to Lu Zuomei, Xu Fu
Sea, Jin Xiahong wait hybrid parents coordinate force and Heterotic Groups to summarize [J] kind subworld, 2014,11 (1): 11-13.).
For separate sources parent, (brave referring to 5, Zhao Jinliang, simple great achievement waits difference bolti, Oreochromis aureus to 5 brave grades
The hereditary feature of parental population analyzes [J] guangdong agricultural science, 2013,40 (15): 158-162.Wu Yong, Zhao Jinliang, letter are big
Industry waits different groups bolti, Oreochromis aureus combined hybrid effect to compare [J] fresh water fishery, 2014,44 (2):
Bolti, the analysis of Oreochromis aureus different groups parent's hereditary feature, different boltis 67-162.) are carried out
♀ × Oreochromis aureusParental combination cross experiment finds JeFu luoluo fish heredity homozygosity highest, with Xin Jifuluo
Non- fish ♀ is maternal cross combination output of seedling than being that maternal combination is high with group, the Sudan ♀；JeFu luoluo fish ♀ with color
Arrange groupGenetic distance is maximum, and the output of seedling of the combination is also above other cross combinations.At the same time, hybrid vigour
Coordinate force is influenced between by parent, and combining ability effect is additive action of genes, less to be influenced by genetic background, combining ability effect
High hybrid parent tends to make a series of excellent combination.Combining Ability of Different Parental Varieties research (referring to Zeng Cong, is opened in megalobrama amblycephala
Credit, Cao Xiaojuan wait the coordinate force and microsatellite marker prediction [J] aquatic product journal of 3 effect of geographic population hybridization of megalobrama amblycephala,
2012,36 (6): 810-814.), (referring to Wang Ping Qian, gu wei, kao hui jiang waits pre- using coordinate force and microsatellite marker to rainbow trout
Survey heterosis, hybrid vigor [J] Chinese aquatic science between rainbow trout strain, 2009,16 (2): 206-213.) etc. in fish hybridizations breeding
It has been reported that, Combining Ability of Different Parental Varieties is the important parameter of cross combination apolegamy, and combining ability effect height is then conducive to hybrid vigour embodiment.
Multiple cross be by multistage hybridization between more than two separate sources parents, it is excellent with the synthesis hybrid for improving its offspring
Gesture (referring to Zhao Hongwen, Xie Rongqing, Zhou Mingliang yak multiple cross composite test studies the agriculture journal in the southwest [J], and 2014,27
(1):393-397.).Multiple cross is widely applied (referring to blue trip's great waves, huang lusheng, Ma Junwu bis- in livestock breeding and production
Paint face pig Quaternary hybrid and duroc-landrace ternary hybrid combination comparative test report [J] Southwest University for Nationalities journal, 2004,
30 (6): 742-744.), (referring to Zhao Ruilian, Xu Yueying, hu xingsen Wan Nan flower pig are polynary miscellaneous for the raising including litter size of offspring
Hand over experimental study [J] Journal of Anhui Agricultural College, 1989,6 (3): 202-206.).In fish, three way cross carp [(scattered mirror carp
♀ × Singuo Red Carp) ♀ × money river open country carp] be successfully example (referring to Xu Yao moral three way cross carp [(scattered mirror carp ♀ ×
Singuo Red Carp ♀) ♀ × money river open country carp ♂] first generation of hybrid heterosis, hybrid vigor and research [J] Hunan aquatic products for utilizing, 1989,5:67-
70.)。
Summary of the invention
A kind of polynary parent of Tilapia mossambica is provided it is an object of the invention to overcome the shortcomings of the prior art place
Cross-breeding method, the present invention also provides Tilapia mossambicas obtained by adopting the seed production method.
To achieve the above object, the technical solution taken: a kind of polynary parents producing method for seed of Tilapia mossambica, the system
Kind of method the following steps are included:
(1) Xin Jifu strain bolti and the Sudan's strain bolti intraspecific hybridization is carried out to breed to obtain son one
For bolti；
It (2) is female parent with the raun in first filial generation bolti obtained in step (1), the hero in Oreochromis aureus
Fish is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Preferably, intraspecific hybridization described in the step (1) is with the raun in Xin Jifu strain bolti for mother
This, is carried out using the milter in the Sudan strain bolti as male parent.
Preferably, intraspecific hybridization described in the step (1) is with the milter in Xin Jifu strain bolti for father
This, is maternal carry out with the raun in the Sudan strain bolti.
Preferably, Oreochromis aureus described in the step (2) is Maoming strain Oreochromis aureus.
Preferably, Oreochromis aureus described in the step (2) is Israel's strain Oreochromis aureus.
Preferably, Oreochromis aureus described in the step (2) is with the raun in the strain Oreochromis aureus of Maoming
For female parent, the first filial generation benefit difficult to understand that intraspecific hybridization is bred is carried out by male parent of the milter in Israel's strain Oreochromis aureus
Sub- Tilapia mossambica.
Preferably, Oreochromis aureus described in the step (2) is with the milter in the strain Oreochromis aureus of Maoming
It is the maternal first filial generation Austria benefit for carrying out intraspecific hybridization and breeding with the raun in Israel's strain Oreochromis aureus for male parent
Sub- Tilapia mossambica.
The present invention also provides a kind of Tilapia mossambicas obtained using the method production of hybrid seeds described above.
The beneficial effects of the present invention are: the present invention provides a kind of polynary parents producing method for seed of Tilapia mossambica, use
The seeding technique that Tilapia mossambica intraspecific hybridization and interspecific hybridization combine gathers together various merits, improves going out for Tilapia mossambica
Seedling amount and male rate of offspring, to improve the yield and benefit of Rofe fish culture；The present invention also provides use the production of hybrid seeds side
The Tilapia mossambica that method obtains, the Tilapia mossambica have many advantages, such as that output of seedling height and male rate of offspring are high.
Detailed description of the invention
Fig. 1 is the water temperature variation diagram of Rofe fish test in the embodiment of the present invention 1；
Fig. 2 is histogram of seedling emergence of the Rofe fish test during 16-June 15 May in the embodiment of the present invention 1,
Wherein the right annotation；
Fig. 3 is the histogram of male rate in different hybrid combinations of Rofe fish test in the embodiment of the present invention 1；
Wherein " new Ji ♀ × Maoming in Fig. 2 and Fig. 3" indicate Xin Jifu ♀ × Maoming, " (new Soviet Union) ♀ × with" indicate
(Xin Jifu ♀ × the Sudan) ♀ × with, " (new Soviet Union) ♀ × cyclopentadienyl" indicate (Xin Jifu ♀ × the Sudan) ♀ × Maoming, " (Soviet Union
Newly) ♀ × with" indicate (the Sudan ♀ × Xin Jifu) ♀ × with, " (Su Xin) ♀ × cyclopentadienyl" indicate (the Sudan ♀ × Xin Jifu
) ♀ × Maoming, " (new Soviet Union) ♀ × (with cyclopentadienyl)" indicate (Xin Jifu ♀ × the Sudan) ♀ × (Israel ♀ × Maoming),
" (new Soviet Union) ♀ × (cyclopentadienyl with)" indicate (Xin Jifu ♀ × the Sudan) ♀ × (Maoming ♀ × Israel), " (Su Xin) ♀ ×
(with cyclopentadienyl)" indicate (the Sudan ♀ × Xin Jifu) ♀ × (Israel ♀ × Maoming), " (Su Xin) ♀ × (cyclopentadienyl with)" table
Show (the Sudan ♀ × Xin Jifu) ♀ × (Maoming ♀ × Israel)。
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
1 materials and methods
1.1 test material
Rofe fish test parent is provided by Weiye Tilapia Breeding Ground in Maoming city, Guangdong Province, including Xin Jifu strain Buddhist nun sieve
(2008 from the domestic White Nile of the Sudan and green Buddhist nun for Tilapia mossambica (Shanghai Ocean University breeding F15), the Sudan strain bolti
Luo He intersection introduces), Maoming strain Oreochromis aureus (being introduced by guangzhou aquatic products research institute for 1981) and Israel's product
It is Oreochromis aureus (being introduced from Zhujiang River aquatic products research institute for 2003, be derived from Israel).In July, 2013, it is prepared for Buddhist nun respectively
Sieve Tilapia mossambica, Oreochromis aureus kind in two-way cross parent, bolti: Xin Jifu ♀ × the Sudan, the Sudan ♀ × new
Ji Fu, Oreochromis aureus: Israel ♀ × Maoming, Maoming ♀ × Israel.After August emergence, respectively in outdoor cement
(5 × 9 × 1.5m) is cultivated in pond, feeds compound feed of tilapia, and per day feeding volume is 5%.Mid-November goes to indoor cart
Between carry out overwintering culture in cement pit.In March, 2014, then go to and continue reinforced cultivating in outdoor tank.In the present embodiment,
The ♀ indicates raun, describedIndicate milter, the Xin Jifu indicates Xin Jifu strain bolti (Shanghai Ocean University
Breeding F15), the Sudan indicates that the Sudan's strain bolti (is handed over for 2008 from the domestic White Nile of the Sudan and the green Nile
Introduced at remittance), described Maoming indicates Maoming strain Oreochromis aureus (being introduced by guangzhou aquatic products research institute within 1981), described
Israel indicates Israel's strain Oreochromis aureus (introducing from Zhujiang River aquatic products research institute within 2003, be derived from Israel).At this
In embodiment, the Xin Jifu ♀ × the SudanIt indicates with the raun in Xin Jifu strain bolti to be female parent, the Sudan's strain
Milter in bolti carries out kind of an interior two-way cross for male parent and breeds to obtain first filial generation bolti；The Sudan ♀ ×
Xin JifuIndicate that the raun in the Sudan strain bolti is using the milter in Xin Jifu strain bolti as male parent
Female parent carries out kind of an interior two-way cross and breeds to obtain first filial generation bolti；Described Israel ♀ × MaomingIt indicates with Maoming product
It is milter in Oreochromis aureus is male parent, the raun in Israel's strain Oreochromis aureus is maternal to carry out intraspecific hybridization
Breed obtained first filial generation Oreochromis aureus；Maoming ♀ × the IsraelIt indicates in the strain Oreochromis aureus of Maoming
Raun be female parent, the milter in Israel's strain Oreochromis aureus is the first filial generation that male parent carries out that intraspecific hybridization is bred
Oreochromis aureus.
1.2 test method
On April 21st, 2014, select that neat specification, the binary tilapia of gonadal maturation be female, male parent population respectively, in cyclopentadienyl
Breeding experiment of multiple hybrid combinations is carried out in small Tu Chi (5 × 4 × 2m) in name city great achievement Tilapia mossambica seed multiplication farm, is provided with 4 altogether
Three way cross combination: (Xin Jifu ♀ × the Sudan) ♀ × Israel, (Xin Jifu ♀ × the Sudan) ♀ × Maoming, (the Sudan
♀ × Xin Jifu) ♀ × Israel, (the Sudan ♀ × Xin Jifu) ♀ × Maoming；4 Quaternary hybrids: (Xin Jifu
♀ × the Sudan) ♀ × (Israel ♀ × Maoming), (Xin Jifu ♀ × the Sudan) ♀ × (Maoming ♀ × Israel)、
(the Sudan ♀ × Xin Jifu) ♀ × (Israel ♀ × Maoming), (the Sudan ♀ × Xin Jifu) ♀ × (Maoming ♀ × with color
Column), and Xin Jifu ♀ × Maoming is setTwo-way cross is as a control group (table 1).Each test tank launch 24 tail of raun,
15 tail of milter, size of male and female fish is between 500~550g.In the present embodiment, described (the Xin Jifu ♀ × the Sudan) ♀ × with color
ColumnIt indicates with the raun in Xin Jifu strain bolti to be female parent, the milter in the Sudan strain bolti is male parent
Intraspecific hybridization is carried out to breed to obtain first filial generation bolti；It then is mother with the raun in obtained first filial generation bolti
This, the milter in Israel's strain Oreochromis aureus is that male parent progress interspecific hybridization obtains hybrid generation.Other similar expression
Mode such as (Xin Jifu ♀ × the Sudan) ♀ × Maoming, (the Sudan ♀ × Xin Jifu) ♀ × Israel, (the Sudan ♀ × new lucky
It is rich) ♀ × Maoming, (Xin Jifu ♀ × the Sudan) ♀ × (Israel ♀ × Maoming), (Xin Jifu ♀ × the Sudan)♀
× (Maoming ♀ × Israel), (the Sudan ♀ × Xin Jifu) ♀ × (Israel ♀ × Maoming), (the Sudan ♀ × new lucky
It is rich) ♀ × (Maoming ♀ × Israel)Meaning can be according to (Xin Jifu ♀ × the Sudan) ♀ × IsraelMeaning solution
It releases and analogizes.
After combo, daily 6:00,18:00 timing bait throwing in, and water water temperature is measured, carry out water level, water quality management.Daily edge
Pool side observes emergence and fishes out seedling after discovery emergence with dab net immediately, count the output of seedling of Different Cross Combinations.Separately take not
With the fry of cross experiment group, individually cultivated in cement culture pond (4 × 7 × 1.5m) respectively.When fry overall length 3cm or more,
Every group of 70 tail of random sampling carries out sex identification using acetic red dyeing, referring in particular to " Tilapia mossambica if three parallel groups
Fingerling sex appraisal method " (SC/T1105-2007) execution, count male ratio.The list of heretofore described output of seedling
Position is tail.
The statistical result of output of seedling and male ratio is analyzed through Excel and 20 software of SPSS, and carries out the significance of difference
It examines.General combining ability analysis, calculation method reference literature are carried out to its parent according to the output of seedling of cross combination and male ratio
(implement [J] tropical crops journal referring to the SAS of han hanpeng diallel cross method of breeding coordinate force statistical analysis (univariate),
2001,22 (4): 72-77. Li Zhe, Shi Shangli, Wang Hong alfalfa multiple cross offspring's elite plant strain screening study [J] plants
Object genetic resources journal, 2013,14 (5): 945-953.), parent's superiority and inferiority is assessed according to general combining ability value size, simultaneously
Parent effect is analyzed to the contribution of filial generation.
2 results
During test, small soil pond average temperature is fluctuated at 23.3~35.6 DEG C.Water temperature fluctuation before May 14 is very big,
6.2 DEG C of morning and evening maximum temperature difference, water temperature later is opposite to be increased, and fluctuating range becomes smaller, and generally water temperature is more stable, as shown in Figure 1.
The general combining ability of 2.1 three way cross, the output of seedling of Quaternary hybrid and hybrid parent is analyzed
From on May 13rd, 2014, fry output has been observed in test tank successively, it is primary at interval of two days fishing seedlings.Wherein,
Results of seedling emergence during 16-June 15 May is shown in Fig. 2 and table 1.The average daily output of seedling of three way cross combination is 190~258
Tail is significantly higher than control group-binary hybrid combination (P < 0.05).In three way cross group, (Xin Jifu ♀ × the Sudan) ♀ × with color
ColumnThe average daily output of seedling highest of combination, (the Sudan ♀ × Xin Jifu significant (P < 0.05) with other three group differences) ♀ × cyclopentadienyl
NameThe average daily output of seedling of combination is minimum.(Xin Jifu ♀ × the Sudan) ♀ be maternal cross combination average daily output of seedling it is significant
Higher than (the Sudan ♀ × Xin Jifu) cross combination of the ♀ for female parent, IsraelFor the average daily output of seedling of the cross combination of male parent
Higher than MaomingFor the cross combination of male parent.
The statistical results of seedling emergence of 1 Different Cross Combinations of table
The average daily output of seedling of Quaternary hybrid is slightly above binary hybrid group.In quaternionic breeding group, (Xin Jifu ♀ × the Sudan) ♀ × (Israel ♀ × Maoming)The average daily output of seedling highest (P < 0.05) of combination, the average daily emergence of other three combinations
Difference is not significant between amount.(Xin Jifu ♀ × the Sudan) ♀ is that maternal Quaternary hybrid is higher than (the Sudan ♀ × Xin Jifu)
♀ is maternal cross combination.Compared with three way cross, the output of seedling of the Quaternary hybrid of identical female parent is lower than three way cross
Combination.
The general combining ability analysis of hybrid parent offspring's output of seedling is shown in Table 2.(Xin Jifu ♀ × the Sudan) ♀ is maternal one
Well matched resultant force (effect) highest, male parent israelGeneral combining ability (effect) highest.(Xin Jifu ♀ × the Sudan) ♀ × with
Color columnCombined specific combining ability highest.
Table 2 in terms of affecting the emergence amount analyzes hybrid parent general combining ability
2.2 three way cross, Quaternary hybrid filial generation male ratio
The measurement result of male rate in different hybrid combinations is shown in Fig. 3 and table 3.Three way cross male rate of offspring is 83.8%
~90.0%, it is significantly higher than binary hybrid control group (P < 0.05).In three way cross combination, (Xin Jifu ♀ × the Sudan)♀×
MaomingMale rate of offspring highest.
The test results of male rate of offspring of 3 Different Cross Combinations of table
Quaternionic breeding male rate of offspring is 76.7%~82.87%, (Xin Jifu ♀ × the Sudan) ♀ × (Maoming ♀ × with
Color column)Male rate of offspring highest, (Xin Jifu ♀ × the Sudan) ♀ × (Israel ♀ × Maoming)Combine it is minimum, separately
Outer two group difference is not significant.The male ratio of Quaternary hybrid offspring is below three way cross combination.
The analysis of hybrid parent male rate of offspring general combining ability is shown in Table 4.(Xin Jifu ♀ × the Sudan) ♀ is maternal general
Coordinate force highest, and in male parent, general cooperation (effect) highest of Maoming strain, Israel's strain is taken second place, intraspecific hybridization parent
It is lower.Very from the general combining ability difference between the general combining ability (effect) for the male ratio analysis parent for influencing offspring, each male parent
Greatly, maternal difference is smaller.
Table 4 in terms of affecting the male rate of offspring analyzes hybrid parent general combining ability
Embodiment 2
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
It (1) is female parent with the raun in Xin Jifu strain bolti, the milter in the Sudan strain bolti is
Male parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
It (2) is female parent, Maoming strain Ao Liya Rofe with the raun in first filial generation bolti obtained in step (1)
Milter in fish is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Embodiment 3
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
It (1) is female parent with the raun in Xin Jifu strain bolti, the milter in the Sudan strain bolti is
Male parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
It (2) is female parent, strain Austria, Israel Leah sieve with the raun in first filial generation bolti obtained in step (1)
Milter in non-fish is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Embodiment 4
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
(1) using the milter in Xin Jifu strain bolti as male parent, the raun in the Sudan strain bolti is
Female parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
It (2) is female parent, Maoming strain Ao Liya Rofe with the raun in first filial generation bolti obtained in step (1)
Milter in fish is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Embodiment 5
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
(1) using the milter in Xin Jifu strain bolti as male parent, the raun in the Sudan strain bolti is
Female parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
It (2) is female parent, strain Austria, Israel Leah sieve with the raun in first filial generation bolti obtained in step (1)
Milter in non-fish is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Embodiment 6
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
It (1) is female parent with the raun in Xin Jifu strain bolti, the milter in the Sudan strain bolti is
Male parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
It (2) is female parent with the raun in the strain Oreochromis aureus of Maoming, the hero in Israel's strain Oreochromis aureus
Fish is the first filial generation Oreochromis aureus that male parent carries out that intraspecific hybridization is bred；
It (3) is female parent, cyclopentadienyl obtained in step (2) with the raun in first filial generation bolti obtained in step (1)
Milter in name strain Oreochromis aureus is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Embodiment 7
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
(1) using the milter in Xin Jifu strain bolti as male parent, the raun in the Sudan strain bolti is
Female parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
It (2) is female parent with the raun in the strain Oreochromis aureus of Maoming, the hero in Israel's strain Oreochromis aureus
Fish is the first filial generation Oreochromis aureus that male parent carries out that intraspecific hybridization is bred；
It (3) is female parent, cyclopentadienyl obtained in step (2) with the raun in first filial generation bolti obtained in step (1)
Milter in name strain Oreochromis aureus is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Embodiment 8
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
It (1) is female parent with the raun in Xin Jifu strain bolti, the milter in the Sudan strain bolti is
Male parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
(2) female in Israel's strain Oreochromis aureus using the milter in the strain Oreochromis aureus of Maoming as male parent
Fish is the maternal first filial generation Oreochromis aureus for carrying out intraspecific hybridization and breeding；
It (3) is female parent, cyclopentadienyl obtained in step (2) with the raun in first filial generation bolti obtained in step (1)
Milter in name strain Oreochromis aureus is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Embodiment 9
A kind of embodiment of the hybrid seed production method for tilapia multiple parents disclosed by the invention, the producing method for seed include following
Step:
(1) using the milter in Xin Jifu strain bolti as male parent, the raun in the Sudan strain bolti is
Female parent carries out intraspecific hybridization and breeds to obtain first filial generation bolti；
(2) female in Israel's strain Oreochromis aureus using the milter in the strain Oreochromis aureus of Maoming as male parent
Fish is the maternal first filial generation Oreochromis aureus for carrying out intraspecific hybridization and breeding；
It (3) is female parent, cyclopentadienyl obtained in step (2) with the raun in first filial generation bolti obtained in step (1)
Milter in name strain Oreochromis aureus is that male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should
Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention
And range.
Claims (1)
1. a kind of polynary parents producing method for seed of Tilapia mossambica, which is characterized in that the producing method for seed the following steps are included:
(1) Xin Jifu strain bolti and the Sudan's strain bolti intraspecific hybridization is carried out to breed to obtain first filial generation Buddhist nun
Sieve Tilapia mossambica；
It (2) is female parent with the raun in first filial generation bolti obtained in step (1), the milter in Oreochromis aureus is
Male parent carries out interspecific hybridization breeding, obtains hybrid generation, the as described Tilapia mossambica；
Intraspecific hybridization described in step (1) is with the raun in Xin Jifu strain bolti for female parent, the Sudan strain Buddhist nun sieve
Milter in Tilapia mossambica is what male parent carried out；
Oreochromis aureus described in step (2) is Israel's strain Oreochromis aureus.
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| CN111109163B (en) * | 2020-02-14 | 2022-03-29 | 江苏农林职业技术学院 | Breeding method of commercial snakehead |
| CN111286547A (en) * | 2020-03-26 | 2020-06-16 | 茂名市伟业罗非鱼良种场 | Tilapia and microsatellite identification primer and method for genetic diversity of Tilapia |
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|---|---|---|---|---|
| CN101292633A (en) * | 2007-04-28 | 2008-10-29 | 李思发 | Seedling production method for hybridization fish-Ji'ao tilapia |
| CN101518216A (en) * | 2009-04-03 | 2009-09-02 | 中国水产科学研究院珠江水产研究所 | Method for seed production of O.mossambicus and O.hornorum |
| CN102630617A (en) * | 2012-04-20 | 2012-08-15 | 中国水产科学研究院黄海水产研究所 | Breed conservation, screening and breed production method in tilapia complete set lines as well as between two lines of complete sets |
| CN102696520A (en) * | 2012-07-02 | 2012-10-03 | 广西壮族自治区水产研究所 | Method for producing seeds of new Nero-Aurea tilapia |
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| JP2011067187A (en) * | 2009-09-25 | 2011-04-07 | Kagawa Prefecture | Artificial insemination method of internal fertilization fish of marine fish |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101292633A (en) * | 2007-04-28 | 2008-10-29 | 李思发 | Seedling production method for hybridization fish-Ji'ao tilapia |
| CN101518216A (en) * | 2009-04-03 | 2009-09-02 | 中国水产科学研究院珠江水产研究所 | Method for seed production of O.mossambicus and O.hornorum |
| CN102630617A (en) * | 2012-04-20 | 2012-08-15 | 中国水产科学研究院黄海水产研究所 | Breed conservation, screening and breed production method in tilapia complete set lines as well as between two lines of complete sets |
| CN102696520A (en) * | 2012-07-02 | 2012-10-03 | 广西壮族自治区水产研究所 | Method for producing seeds of new Nero-Aurea tilapia |
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Effective date of registration: 20210630 Address after: 525000 office building of Weiye tilapia breeding farm, Xiadong village, Gongguan Town, Maonan District, Maoming City, Guangdong Province Patentee after: Guangdong Weiye improved tilapia breeding Co.,Ltd. Patentee after: Shanghai Ocean University Address before: 525024 Xiadong village, Gongguan Town, Maonan District, Maoming City, Guangdong Province Patentee before: MAOMING CITY WEIYE TILAPIA SEED FIELD Patentee before: Shanghai Ocean University |