CN104542397A - Multi-parental hybrid breeding method of tilapia - Google Patents
Multi-parental hybrid breeding method of tilapia Download PDFInfo
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- CN104542397A CN104542397A CN201410854647.0A CN201410854647A CN104542397A CN 104542397 A CN104542397 A CN 104542397A CN 201410854647 A CN201410854647 A CN 201410854647A CN 104542397 A CN104542397 A CN 104542397A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention provides a breeding method of tilapia. The breeding method comprises the steps of performing intra specific hybrid breeding for new Gift tilapia and Sudan tilapia to obtain the first filial generation tilapia; performing interspecific hybrid breeding by using raun of the first filial generation tilapia as a female parent and milter of blue tilapia as a male parent to obtain the hybrid generation which is the tilapia. According to the method, the breeding technology combining specific hybrid breeding and interspecific hybrid breeding of tilapia is adopted; various outstanding properties are collected; therefore, the output of fries and male rate of the later generations are increased, and as a result, the breeding output and benefits of tilapia are increased. The invention further provides tilapia bred through the breeding method; the tilapia has the advantages of being high in output of fries and male rate of the later generations.
Description
Technical field
The present invention relates to the producing method for seed of a kind of Tilapia mossambica, be specifically related to the producing method for seed of a kind of multiple cross Tilapia mossambica.
Background technology
Tilapia mossambica is one of principal item of China's freshwater aquiculture, be also important outlet aquatic products kind (see Li Sifa. the development prospect of China's Tilapia mossambica industry and bottleneck problem [J]. science is breeded fish, 2003,12 (9): 3-5.).Because female, milter exist significant difference between growing, single (hero) property cultivation has obvious economic benefit.Interspecific cross is biological method the most safe, practical during the nursery of Tilapia mossambica scale unisexuality is produced, and a large number of experiments show that, bolti Oreochromis niloticus ♀ × Oreochromis aureus O.aureus
the heterosis, hybrid vigor that hybrid combination obtains is the most obvious, and first-filial generation not only male ratio is high, and fast growth (see Li Sifa, Chen Lin, Cai Wanqi. lucky Tilapia mossambica difficult to understand (JeFu luoluo fish ♀ × Oreochromis aureus
) and RAPD, SSR comparative analysis [J] of hereditary difference of 4 nearly edge genotype Tilapia mossambicas. aquatic product journal, 2008,32 (5): 657-664.Xiao Wei, Li great Yu, Yang Hong, etc. the growth comparative study [J] of four kinds of hybrid combination Sarotherodon sps and parent thereof. aquatile journal, 2012,36 (5): 905-912.).But in actual production, bolti ♀ × Oreochromis aureus
also there is the phenomenon that output of seedling is on the low side, male ratio is unstable in hybridization, have impact on Buddhist nun's heterosis, hybrid vigor utilization difficult to understand to a certain extent and promote with hybrid.
Hybrid vigour generally depends on amphilepsis purity height with Relative Hereditary difference size (see Lu Zuomei, Xu Fuhai, Jin Xiahong, etc. hybrid parents coordinate force and Heterotic Groups general introduction [J]. plant subworld, 2014,11 (1): 11-13.).For separate sources parent, Wu Yong etc. (brave see 5, Zhao Jinliang, simple great achievement, etc. the hereditary feature analysis [J] of different bolti, Oreochromis aureus parental population. guangdong agricultural science, 2013,40 (15): 158-162.Wu Yong, Zhao Jinliang, letter great achievement, Deng. different groups bolti, Oreochromis aureus combined hybrid effectiveness comparison [J]. fresh water fishery, 2014,44 (2): 67-162.) carried out bolti, Oreochromis aureus different groups parent hereditary feature has been analyzed, different bolti ♀ × Oreochromis aureus
parental combination cross experiment, find that JeFu luoluo fish heredity homozygosity is the highest, the hybrid combination output of seedling being female parent with JeFu luoluo fish ♀ is higher than the combination being female parent with colony of the Sudan ♀; JeFu luoluo fish ♀ and colony of Israel
genetic distance is maximum, and the output of seedling of this combination is also higher than other hybrid combination.Meanwhile, hybrid vigour also affects by coordinate force between parent, and combining ability effect is gene cloning and espression, and the less impact by genetic background, the hybrid strain that combining ability effect is high often can make a series of excellent combination.Combining Ability of Different Parental Varieties research at megalobrama amblycephala (see Zeng Cong, Zhang Yao, Cao little Juan, Deng. the coordinate force of megalobrama amblycephala 3 geographical population crossbreeding effects and microsatellite marker prediction [J]. aquatic product journal, 2012, 36 (6): 810-814.), rainbow trout is (see Wang Ping Qian, Gu Wei, Gao Huijiang, Deng. utilize the heterosis, hybrid vigor [J] between coordinate force and microsatellite marker prediction rainbow trout strain. Chinese aquatic science, 2009, 16 (2): 206-213.) etc. have been reported in fish hybridization breeding, Combining Ability of Different Parental Varieties is the important parameter of hybrid combination apolegamy, combining ability effect height is then conducive to hybrid vigour to be embodied.
Multiple cross is by multistage hybridization between two or more separate sources parent, to improve the overall transport network of its offspring (see Zhao Hongwen, Xie Rongqing, Zhou Mingliang. yak multiple cross composite test research [J]. southwestern agriculture journal, 2014,27 (1): 393-397.).Multiple cross herding breeding produce on extensive use (see blue trip great waves, Huang Lusheng, fiber crops fine horse is military. and Erhualian Quaternary hybrid and DLY three way cross combine comparative test report [J]. Southwest University for Nationalities journal, 2004,30 (6): 742-744.), comprise the raising of offspring's litter size (see Zhao Ruilian, Xu Yueying, Hu Xingsen. Wan Nan flower pig multiple cross experimental study [J]. Anhui Agriculture College's journal, 1989,6 (3): 202-206.).In fish, three way cross carp [(scattered mirror carp ♀ × Singuo Red Carp
) the wild carp in ♀ × money river
] be successfully example (see Xu Yaode. the research [J] of three way cross carp [(scattered mirror carp ♀ × Singuo Red Carp ♀) ♀ × money river open country carp ♂] first generation of hybrid heterosis, hybrid vigor and utilization. Hunan aquatic products, 1989,5:67-70.).
Summary of the invention
The object of the invention is to overcome weak point that prior art exists and provide the polynary parents producing method for seed of a kind of Tilapia mossambica, present invention also offers the Tilapia mossambica adopting described producing method for seed to obtain.
For achieving the above object, the technical scheme taked: the polynary parents producing method for seed of a kind of Tilapia mossambica, described producing method for seed comprises the following steps:
(1) Xin Jifu strain bolti and the Sudan strain bolti are carried out intraspecific hybridization to breed and obtain first filial generation bolti;
(2) with the raun in the first filial generation bolti obtained in step (1) for maternal, the milter in Oreochromis aureus is that male parent carries out interspecific cross breeding, obtains hybrid generation, is described Tilapia mossambica.
Preferably, intraspecific hybridization described in described step (1) is with the raun in Xin Jifu strain bolti for female parent, with the milter in the Sudan strain bolti for male parent is carried out.
Preferably, intraspecific hybridization described in described step (1) is with the milter in Xin Jifu strain bolti for male parent, with the raun in the Sudan strain bolti for female parent carries out.
Preferably, Oreochromis aureus described in described step (2) is Maoming strain Oreochromis aureus.
Preferably, Oreochromis aureus described in described step (2) is Israel's strain Oreochromis aureus.
Preferably, Oreochromis aureus described in described step (2) is with the raun in the strain Oreochromis aureus of Maoming for female parent, carries out intraspecific hybridization breed the first filial generation Oreochromis aureus obtained with the milter in Israel's strain Oreochromis aureus for male parent.
Preferably, Oreochromis aureus described in described step (2) is with the milter in the strain Oreochromis aureus of Maoming for male parent, breeds the first filial generation Oreochromis aureus obtained with the raun in Israel's strain Oreochromis aureus for female parent carries out intraspecific hybridization.
Present invention also offers a kind of Tilapia mossambica adopting the method production of hybrid seeds described above to obtain.
Beneficial effect of the present invention is: the invention provides the polynary parents producing method for seed of a kind of Tilapia mossambica, adopt the seeding technique that Tilapia mossambica intraspecific hybridization and interspecific cross combine, various merit is met and, improve output of seedling and offspring's male ratio of Tilapia mossambica, thus improve output and the benefit of Tilapia mossambica cultivation; Present invention also offers the Tilapia mossambica adopting described producing method for seed to obtain, this Tilapia mossambica has output of seedling height and offspring's male ratio advantages of higher.
Accompanying drawing explanation
Fig. 1 is the water temperature variation diagram of Tilapia mossambica test in embodiments of the invention 1;
Fig. 2 is the output of seedling block diagram of Tilapia mossambica test during 16-June 15 May, wherein the right annotations and comments in embodiments of the invention 1;
Fig. 3 is the Different Cross Combinations offspring male ratio block diagram of Tilapia mossambica test in embodiments of the invention 1;
Wherein " new lucky ♀ × Maoming in Fig. 2 and Fig. 3
" represent Xin Jifu ♀ × Maoming
, " (newly reviving) ♀ × with
" represent (Xin Jifu ♀ × the Sudan
) ♀ × with
, " (newly reviving) ♀ × luxuriant
" represent (Xin Jifu ♀ × the Sudan
) ♀ × Maoming
, " (Su Xin) ♀ × with
" represent (the Sudan ♀ × Xin Jifu
) ♀ × with
, " (Su Xin) ♀ × luxuriant
" represent (the Sudan ♀ × Xin Jifu
) ♀ × Maoming
, " (newly reviving) ♀ × (with luxuriant)
" represent (Xin Jifu ♀ × the Sudan
) ♀ × (Israel ♀ × Maoming
)
, " (newly revive) ♀ × (luxuriant with)
" represent (Xin Jifu ♀ × the Sudan
) ♀ × (Maoming ♀ × Israel
)
, " (Su Xin) ♀ × (with luxuriant)
" represent (the Sudan ♀ × Xin Jifu
) ♀ × (Israel ♀ × Maoming
)
, " (Su Xin) ♀ × (luxuriant with)
" represent (the Sudan ♀ × Xin Jifu
) ♀ × (Maoming ♀ × Israel
)
.
Embodiment
For better the object, technical solutions and advantages of the present invention being described, below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
1 materials and methods
1.1 test material
Tilapia mossambica test parent provide by Maoming City great achievement Tilapia mossambica seed multiplication farm, comprise Xin Jifu strain bolti (Shanghai Ocean University seed selection F15), the Sudan strain bolti (introducing from the domestic White Nile of the Sudan and blue or green Nile intersection for 2008), Maoming strain Oreochromis aureus (being introduced by Guangzhou aquatic products research institute for 1981) and Israel's strain Oreochromis aureus (introduce from Zhujiang River aquatic products research institute, come from Israel for 2003).In July, 2013, two-way cross parent in the kind having prepared bolti, Oreochromis aureus respectively, bolti: Xin Jifu ♀ × the Sudan
, the Sudan ♀ × Xin Jifu
, Oreochromis aureus: Israel ♀ × Maoming
, Maoming ♀ × Israel
.After emerging August, in outdoor tank-culture, (5 × 9 × 1.5m) cultivates respectively, and compound feed of tilapia of throwing something and feeding, per day feeding volume is 5%.Go to mid-November between indoor cart and carry out overwintering culture in cement pit.In March, 2014, then go in outdoor tank-culture and continue reinforced cultivating.In the present embodiment, described ♀ represents raun, described in
represent milter, described Xin Jifu represents Xin Jifu strain bolti (Shanghai Ocean University seed selection F15), described the Sudan represents the Sudan strain bolti (introducing from the domestic White Nile of the Sudan and blue or green Nile intersection for 2008), described Maoming represents Maoming strain Oreochromis aureus (being introduced by Guangzhou aquatic products research institute for 1981), described Israel represents Israel's strain Oreochromis aureus (introduce from Zhujiang River aquatic products research institute, come from Israel for 2003).In the present embodiment, described Xin Jifu ♀ × the Sudan
represent with the raun in Xin Jifu strain bolti for female parent, the milter in the Sudan strain bolti is that male parent is carried out two-way cross in kind and bred and obtain first filial generation bolti; Described the Sudan ♀ × Xin Jifu
represent with the milter in Xin Jifu strain bolti for male parent, the raun in the Sudan strain bolti is that female parent carries out two-way cross in kind and breeds and obtain first filial generation bolti; Described Israel ♀ × Maoming
represent with the milter in the strain Oreochromis aureus of Maoming for male parent, the raun in Israel's strain Oreochromis aureus is that female parent carries out intraspecific hybridization and breeds the first filial generation Oreochromis aureus obtained; Described Maoming ♀ × Israel
represent with the raun in the strain Oreochromis aureus of Maoming for female parent, the milter in Israel's strain Oreochromis aureus is that male parent is carried out intraspecific hybridization and bred the first filial generation Oreochromis aureus obtained.
1.2 test method
On April 21st, 2014, select female, the male parent population of binary Tilapia mossambica of neat specification, gonadal maturation respectively, in Maoming City great achievement Tilapia mossambica seed multiplication farm, carry out the reproduction test of multiple cross combo in little Tu Chi (5 × 4 × 2m), be provided with 4 three way cross combinations altogether: (Xin Jifu ♀ × the Sudan
) ♀ × Israel
, (Xin Jifu ♀ × the Sudan
) ♀ × Maoming
, (the Sudan ♀ × Xin Jifu
) ♀ × Israel
, (the Sudan ♀ × Xin Jifu
) ♀ × Maoming
; 4 Quaternary hybrids: (Xin Jifu ♀ × the Sudan
) ♀ × (Israel ♀ × Maoming
)
, (Xin Jifu ♀ × the Sudan
) ♀ × (Maoming ♀ × Israel
)
, (the Sudan ♀ × Xin Jifu
) ♀ × (Israel ♀ × Maoming
)
, (the Sudan ♀ × Xin Jifu
) ♀ × (Maoming ♀ × Israel
)
, and Xin Jifu ♀ × Maoming is set
two-way cross is (table 1) as a control group.Each test tank all throws in raun 24 tail, milter 15 tail, and female milter size is between 500 ~ 550g.In the present embodiment, described (Xin Jifu ♀ × the Sudan
) ♀ × Israel
represent with the raun in Xin Jifu strain bolti for female parent, the milter in the Sudan strain bolti be male parent carry out intraspecific hybridization breeding obtain first filial generation bolti; Then with the raun in the first filial generation bolti obtained for female parent, the milter in Israel's strain Oreochromis aureus is that male parent is carried out interspecific cross and obtained hybrid generation.Other similar representations are as (Xin Jifu ♀ × the Sudan
) ♀ × Maoming
, (the Sudan ♀ × Xin Jifu
) ♀ × Israel
, (the Sudan ♀ × Xin Jifu
) ♀ × Maoming
, (Xin Jifu ♀ × the Sudan
) ♀ × (Israel ♀ × Maoming
)
, (Xin Jifu ♀ × the Sudan
) ♀ × (Maoming ♀ × Israel
)
, (the Sudan ♀ × Xin Jifu
) ♀ × (Israel ♀ × Maoming
)
, (the Sudan ♀ × Xin Jifu
) ♀ × (Maoming ♀ × Israel
)
implication can according to (Xin Jifu ♀ × the Sudan
) ♀ × Israel
implication explain analogize.
After combo, 6:00,18:00 timing bait throwing in every day, and measuring cell water water temperature, carry out water level, water quality management.Every day observes along pool side situation of emerging, and discovery drags for seedling with dab net after emerging immediately, the output of seedling of statistics Different Cross Combinations.Separately get the fry of different cross experiment group, cultivate in pond (4 × 7 × 1.5m) at cement respectively and cultivate separately.During fry more than total length 3cm, often organize random sampling 70 tail, if three parallel group, adopt acetic red dyeing to carry out sex identification, concrete reference " Tilapia mossambica fingerling sex appraisal method " (SC/T1105-2007) performs, statistics male ratio.The unit of output of seedling described in the present invention is tail.
The statistics of output of seedling and male ratio is analyzed through Excel and SPSS 20 software, and carries out significance test of difference.According to the output of seedling of hybrid combination and male ratio, general combining ability analysis is carried out to its parent, computational methods reference literature (see Han Hanpeng. the SAS of diallel cross method of breeding coordinate force statistical analysis (monotropic unit) implements [J]. tropical crop journal, 2001, 22 (4): 72-77. Li Zhe, Shi Shangli, Wang Hong. alfalfa multiple cross offspring elite plant strain screening study [J]. plant genetic resources journal, 2013, 14 (5): 945-953.), according to the assessment of general combining ability value size, parent is good and bad, analyze Parent effect to the contribution of filial generation simultaneously.
2 results
Duration of test, little native pond average temperature is 23.3 ~ 35.6 DEG C of fluctuations.Fluctuating temperature before May 14 is very large, sooner or later maximum temperature difference 6.2 DEG C, and water temperature afterwards raises relatively, and fluctuating range diminishes, and water temperature is more stable generally, as shown in Figure 1.
The output of seedling of 2.1 three way crosses, Quaternary hybrid and the general combining ability analysis of hybrid strain
On May 13rd, 2014 rises, and has observed fry output successively, dragged for seedling once at interval of two days in test tank.Wherein, the output of seedling during 16-June 15 May the results are shown in Figure 2 and table 1.The average daily output of seedling of three way cross combination is 190 ~ 258 tails, is significantly higher than control group-two-way cross combination (P<0.05).In three way cross group, (Xin Jifu ♀ × the Sudan
) ♀ × Israel
the average daily output of seedling of combination is the highest, with other three group difference remarkable (P<0.05), and (the Sudan ♀ × Xin Jifu
) ♀ × Maoming
the average daily output of seedling of combination is minimum.(Xin Jifu ♀ × the Sudan
) ♀ is that the average daily output of seedling of maternal hybrid combination is significantly higher than (the Sudan ♀ × Xin Jifu
) ♀ is maternal hybrid combination, Israel
for the average daily output of seedling of the hybrid combination of male parent is higher than Maoming
for the hybrid combination of male parent.
The output of seedling statistics of table 1 Different Cross Combinations
The average daily output of seedling of Quaternary hybrid is a little more than two-way cross group.In four-way cross group, (Xin Jifu ♀ × the Sudan
) ♀ × (Israel ♀ × Maoming
)
the average daily output of seedling the highest (P<0.05) of combination, between the average daily output of seedling of other three combinations, difference is not remarkable.(Xin Jifu ♀ × the Sudan
) ♀ is that maternal Quaternary hybrid is higher than (the Sudan ♀ × Xin Jifu
) ♀ is maternal hybrid combination.Compared with three way cross, the output of seedling of the Quaternary hybrid of identical female parent combines lower than three way cross.
The general combining ability analysis in table 2 of hybrid strain offspring output of seedling.(Xin Jifu ♀ × the Sudan
) ♀ is that maternal general combining ability (effect) is the highest, male parent Israel
general combining ability (effect) the highest.(Xin Jifu ♀ × the Sudan
) ♀ × Israel
the specific combining ability of combination is the highest.
Table 2 is analyzed from affecting output of seedling hybrid strain general combining ability
2.2 three way crosses, Quaternary hybrid filial generation male ratio
The measurement result of Different Cross Combinations offspring male ratio is shown in Fig. 3 and table 3.Three way cross offspring male ratio is 83.8% ~ 90.0%, is significantly higher than two-way cross control group (P<0.05).In three way cross combination, (Xin Jifu ♀ × the Sudan
) ♀ × Maoming
offspring's male ratio is the highest.
Offspring's male ratio measurement result of table 3 Different Cross Combinations
Four-way cross offspring male ratio is 76.7% ~ 82.87%, (Xin Jifu ♀ × the Sudan
) ♀ × (Maoming ♀ × Israel
)
offspring's male ratio the highest, (Xin Jifu ♀ × the Sudan
) ♀ × (Israel ♀ × Maoming
)
combine minimum, other two group differences are not remarkable.The male ratio of Quaternary hybrid offspring all combines lower than three way cross.
Hybrid strain offspring male ratio general combining ability analysis in table 4.(Xin Jifu ♀ × the Sudan
) ♀ is that maternal general combining ability is the highest, and in male parent, the general cooperation (effect) of Maoming strain is the highest, Israel's strain is taken second place, and intraspecific hybridization parent is lower.Analyze the general combining ability (effect) of parent from the male ratio affecting offspring, the general combining ability between each male parent is widely different, and maternal difference is less.
Table 4 is analyzed from affecting offspring's male ratio hybrid strain general combining ability
Embodiment 2
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the raun in Xin Jifu strain bolti for female parent, the milter in the Sudan strain bolti be male parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the raun in the first filial generation bolti obtained in step (1) for maternal, the milter in the strain Oreochromis aureus of Maoming is that male parent carries out interspecific cross breeding, obtains hybrid generation, is described Tilapia mossambica.
Embodiment 3
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the raun in Xin Jifu strain bolti for female parent, the milter in the Sudan strain bolti be male parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the raun in the first filial generation bolti obtained in step (1) for maternal, the milter in Israel's strain Oreochromis aureus is that male parent carries out interspecific cross breeding, obtains hybrid generation, is described Tilapia mossambica.
Embodiment 4
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the milter in Xin Jifu strain bolti for male parent, the raun in the Sudan strain bolti be female parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the raun in the first filial generation bolti obtained in step (1) for maternal, the milter in the strain Oreochromis aureus of Maoming is that male parent carries out interspecific cross breeding, obtains hybrid generation, is described Tilapia mossambica.
Embodiment 5
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the milter in Xin Jifu strain bolti for male parent, the raun in the Sudan strain bolti be female parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the raun in the first filial generation bolti obtained in step (1) for maternal, the milter in Israel's strain Oreochromis aureus is that male parent carries out interspecific cross breeding, obtains hybrid generation, is described Tilapia mossambica.
Embodiment 6
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the raun in Xin Jifu strain bolti for female parent, the milter in the Sudan strain bolti be male parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the raun in the strain Oreochromis aureus of Maoming for female parent, the milter in Israel's strain Oreochromis aureus is that male parent is carried out intraspecific hybridization and bred the first filial generation Oreochromis aureus obtained;
(3) with the raun in the first filial generation bolti obtained in step (1) for maternal, milter in Maoming strain Oreochromis aureus obtained in step (2) is that male parent carries out interspecific cross breeding, obtain hybrid generation, be described Tilapia mossambica.
Embodiment 7
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the milter in Xin Jifu strain bolti for male parent, the raun in the Sudan strain bolti be female parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the raun in the strain Oreochromis aureus of Maoming for female parent, the milter in Israel's strain Oreochromis aureus is that male parent is carried out intraspecific hybridization and bred the first filial generation Oreochromis aureus obtained;
(3) with the raun in the first filial generation bolti obtained in step (1) for maternal, milter in Maoming strain Oreochromis aureus obtained in step (2) is that male parent carries out interspecific cross breeding, obtain hybrid generation, be described Tilapia mossambica.
Embodiment 8
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the raun in Xin Jifu strain bolti for female parent, the milter in the Sudan strain bolti be male parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the milter in the strain Oreochromis aureus of Maoming for male parent, the raun in Israel's strain Oreochromis aureus is that female parent carries out intraspecific hybridization and breeds the first filial generation Oreochromis aureus obtained;
(3) with the raun in the first filial generation bolti obtained in step (1) for maternal, milter in Maoming strain Oreochromis aureus obtained in step (2) is that male parent carries out interspecific cross breeding, obtain hybrid generation, be described Tilapia mossambica.
Embodiment 9
A kind of embodiment of Tilapia mossambica of the present invention polynary parents producing method for seed, described producing method for seed comprises the following steps:
(1) with the milter in Xin Jifu strain bolti for male parent, the raun in the Sudan strain bolti be female parent carry out intraspecific hybridization breeding obtain first filial generation bolti;
(2) with the milter in the strain Oreochromis aureus of Maoming for male parent, the raun in Israel's strain Oreochromis aureus is that female parent carries out intraspecific hybridization and breeds the first filial generation Oreochromis aureus obtained;
(3) with the raun in the first filial generation bolti obtained in step (1) for maternal, milter in Maoming strain Oreochromis aureus obtained in step (2) is that male parent carries out interspecific cross breeding, obtain hybrid generation, be described Tilapia mossambica.
Finally to should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention but not limiting the scope of the invention; although be explained in detail the present invention with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can modify to technical scheme of the present invention or equivalent replacement, and not depart from essence and the scope of technical solution of the present invention.
Claims (8)
1. the polynary parents producing method for seed of Tilapia mossambica, it is characterized in that, described producing method for seed comprises the following steps:
(1) Xin Jifu strain bolti and the Sudan strain bolti are carried out intraspecific hybridization to breed and obtain first filial generation bolti;
(2) with the raun in the first filial generation bolti obtained in step (1) for maternal, the milter in Oreochromis aureus is that male parent carries out interspecific cross breeding, obtains hybrid generation, is described Tilapia mossambica.
2. the polynary parents producing method for seed of Tilapia mossambica according to claim 1, it is characterized in that, intraspecific hybridization described in described step (1) is with the raun in Xin Jifu strain bolti for female parent, and the milter in the Sudan strain bolti is that male parent is carried out.
3. the polynary parents producing method for seed of Tilapia mossambica according to claim 1, it is characterized in that, intraspecific hybridization described in described step (1) is with the milter in Xin Jifu strain bolti for male parent, and the raun in the Sudan strain bolti is that female parent carries out.
4., according to the arbitrary described polynary parents producing method for seed of Tilapia mossambica of claim 1-3, it is characterized in that, Oreochromis aureus described in described step (2) is Maoming strain Oreochromis aureus.
5., according to the arbitrary described polynary parents producing method for seed of Tilapia mossambica of claim 1-3, it is characterized in that, Oreochromis aureus described in described step (2) is Israel's strain Oreochromis aureus.
6. according to the arbitrary described polynary parents producing method for seed of Tilapia mossambica of claim 1-3, it is characterized in that, Oreochromis aureus described in described step (2) is with the raun in the strain Oreochromis aureus of Maoming for female parent, and the milter in Israel's strain Oreochromis aureus is that male parent is carried out intraspecific hybridization and bred the first filial generation Oreochromis aureus obtained.
7. according to the arbitrary described polynary parents producing method for seed of Tilapia mossambica of claim 1-3, it is characterized in that, Oreochromis aureus described in described step (2) is with the milter in the strain Oreochromis aureus of Maoming for male parent, and the raun in Israel's strain Oreochromis aureus is that female parent carries out intraspecific hybridization and breeds the first filial generation Oreochromis aureus obtained.
8. the Tilapia mossambica adopting the method production of hybrid seeds as claimed in claim 1 to obtain.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122415A (en) * | 2018-08-01 | 2019-01-04 | 广西壮族自治区水产科学研究院 | A kind of selection of high numerous type Tilapia mossambica breed system male parent |
| CN111109163A (en) * | 2020-02-14 | 2020-05-08 | 江苏农林职业技术学院 | Breeding method of commercial snakehead |
| CN111286547A (en) * | 2020-03-26 | 2020-06-16 | 茂名市伟业罗非鱼良种场 | Tilapia and microsatellite identification primer and method for genetic diversity of Tilapia |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101292633A (en) * | 2007-04-28 | 2008-10-29 | 李思发 | Seedling production method for hybridization fish-Ji'ao tilapia |
| CN101518216A (en) * | 2009-04-03 | 2009-09-02 | 中国水产科学研究院珠江水产研究所 | Method for seed production of O.mossambicus and O.hornorum |
| JP2011067187A (en) * | 2009-09-25 | 2011-04-07 | Kagawa Prefecture | Artificial insemination method of internal fertilization fish of marine fish |
| CN102630617A (en) * | 2012-04-20 | 2012-08-15 | 中国水产科学研究院黄海水产研究所 | Breed conservation, screening and breed production method in tilapia complete set lines as well as between two lines of complete sets |
| CN102696520A (en) * | 2012-07-02 | 2012-10-03 | 广西壮族自治区水产研究所 | Method for producing seeds of new Nero-Aurea tilapia |
-
2014
- 2014-12-31 CN CN201410854647.0A patent/CN104542397B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101292633A (en) * | 2007-04-28 | 2008-10-29 | 李思发 | Seedling production method for hybridization fish-Ji'ao tilapia |
| CN101518216A (en) * | 2009-04-03 | 2009-09-02 | 中国水产科学研究院珠江水产研究所 | Method for seed production of O.mossambicus and O.hornorum |
| JP2011067187A (en) * | 2009-09-25 | 2011-04-07 | Kagawa Prefecture | Artificial insemination method of internal fertilization fish of marine fish |
| CN102630617A (en) * | 2012-04-20 | 2012-08-15 | 中国水产科学研究院黄海水产研究所 | Breed conservation, screening and breed production method in tilapia complete set lines as well as between two lines of complete sets |
| CN102696520A (en) * | 2012-07-02 | 2012-10-03 | 广西壮族自治区水产研究所 | Method for producing seeds of new Nero-Aurea tilapia |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122415A (en) * | 2018-08-01 | 2019-01-04 | 广西壮族自治区水产科学研究院 | A kind of selection of high numerous type Tilapia mossambica breed system male parent |
| CN111109163A (en) * | 2020-02-14 | 2020-05-08 | 江苏农林职业技术学院 | Breeding method of commercial snakehead |
| CN111109163B (en) * | 2020-02-14 | 2022-03-29 | 江苏农林职业技术学院 | Breeding method of commercial snakehead |
| CN111286547A (en) * | 2020-03-26 | 2020-06-16 | 茂名市伟业罗非鱼良种场 | Tilapia and microsatellite identification primer and method for genetic diversity of Tilapia |
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Effective date of registration: 20210630 Address after: 525000 office building of Weiye tilapia breeding farm, Xiadong village, Gongguan Town, Maonan District, Maoming City, Guangdong Province Patentee after: Guangdong Weiye improved tilapia breeding Co.,Ltd. Patentee after: Shanghai Ocean University Address before: 525024 Xiadong village, Gongguan Town, Maonan District, Maoming City, Guangdong Province Patentee before: MAOMING CITY WEIYE TILAPIA SEED FIELD Patentee before: Shanghai Ocean University |
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