CN101513257A - Method for fermenting functional wheat bran - Google Patents
Method for fermenting functional wheat bran Download PDFInfo
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- CN101513257A CN101513257A CNA200910064358XA CN200910064358A CN101513257A CN 101513257 A CN101513257 A CN 101513257A CN A200910064358X A CNA200910064358X A CN A200910064358XA CN 200910064358 A CN200910064358 A CN 200910064358A CN 101513257 A CN101513257 A CN 101513257A
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Abstract
The invention provides a method for fermenting functional wheat bran. The method comprises the following steps: (1) aspergillus niger is subjected to slant culture, shaking primary culture and shaking secondary culture to obtain aspergillus niger secondary seed solution; (2) microzyme is subjected to slant culture, shaking primary culture and shaking secondary culture to obtain microzyme secondary seed solution; and (3) the aspergillus niger secondary seed solution and the microzyme secondary seed solution are introduced into a fermentation medium prepared by the wheat bran to be fermented, and are finally dried to obtain the functional wheat bran. The functional wheat bran removes various anti-nutritional factors in a vegetable protein raw material, is rich in high-activity wheat bran dietary fiber and high-activity protein, generates small peptide and lactic acid simultaneously when the wheat bran is used, and is easily absorbed by an organism, so that the wheat bran is a high-quality health-care product. The wheat bran realizes effective value addition and utilization of wheat bran while providing health food for people, and has significant social benefit and economical benefit.
Description
Technical field
The invention belongs to fermentation technical field, be specifically related to a kind of fermentation method for producing of functional wheat bran.
Background technology
Wheat bran is the accessory substance of wheat processing, and the output of national annual wheat bran is a very big resource more than 2,000 ten thousand tons, and it contains abundant Cobastab
1With protein etc., higher nutritive value is arranged, but its composition is difficult for being absorbed by body, and mouthfeel is poor, functional outstanding.According to surveying and determination, in wheat bran, protein content is 15.8%, fat is 21.4%, carbohydrate is 41.5%, cellulose is 18%, and the content of vitamin and mineral matter exceeds tens times than flour.But the multiple ANFs that contains in the plant protein material is difficult for being absorbed, and has influenced the value of the edible and additive of wheat bran.In China, wheat bran is mainly as animal and fowl fodder, fermentation medium etc., and is cheap, if can realize the effective increment and the utilization of wheat bran, will have crucial economic benefit and social benefit at present.
Along with development of technology, present existing multiple mode in the application of wheat bran has and uses the wheat bran preparation to close pure glycerine, also have from wheat bran and to extract aleuron, also useful wheat bran is done health care wine or feed addictive, but the process the during enforcement of these application is comparatively complicated, and cost is higher.
Summary of the invention
The fermentation method for producing that the purpose of this invention is to provide a kind of functional wheat bran, product nutritious, absorbed by body easily.
For achieving the above object, the present invention by the following technical solutions: the fermentation method for producing of functional wheat bran may further comprise the steps: (1) aspergillus niger is cultivated the back through the inclined-plane and is obtained aspergillus niger spore; Wash aspergillus niger spore with SPSS and obtain the aspergillus niger spore suspension; The aspergillus niger spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, cultivated 8～30 hours down, obtain the aspergillus niger primary seed solution at 22～40 ℃; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, cultivated 10～50 hours down, obtain aspergillus niger secondary seed solution at 22～40 ℃;
(2) saccharomycete is cultivated the back through the inclined-plane and is obtained the saccharomycete spore; Wash the saccharomycete spore with SPSS and obtain saccharomycete spore suspension; Saccharomycete spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, cultivated 8～30 hours down, obtain the saccharomycete primary seed solution at 22～40 ℃; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, cultivated 10～50 hours down, obtain the saccharomycete secondary seed solution at 22～40 ℃;
(3) aspergillus niger secondary seed solution that step (1) and (2) are obtained and saccharomycete secondary seed solution insert and make fermentation medium by the materials of wheat wheat bran, at 22～40 ℃ of bottom fermentations after 1～30 hour, add lecithinase, the mass concentration that adds the back lecithinase is 100～800ppm, keeping pH value is 6.0～8.0, cultivated 10～98 hours down at 22～40 ℃, oven dry obtains functional wheat bran then.
The component of described fermentation medium is: wheat bran 10～70%, inorganic salts 0.05～2%, all the other are sterilized water.
The access amount of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 0.1～10% of a fermentation medium weight in the described step (3), and the weight ratio of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 1: 1～10.
Cultivated 24～72 hours down at 20～40 ℃ when the inclined-plane is cultivated in described step (1) and the step (2).
The slant medium that the culture medium that adopts when the inclined-plane is cultivated in described step (1) and the step (2) is made for the wheat bran hydrolyzate, this culture medium adopts following method preparation: the water that adds 4～10 times of its weight in wheat bran, smash pulping, the lecithinase that adds slurry weight 0.01～0.05%, 40～80 ℃ of following saccharification 1～4 hour, boil the centrifugal supernatant that gets after 20～40 minutes, every 100ml supernatant adding 1～2g agar.
The component of one-level culture medium is in described step (1) and (2): wheat bran 5～40%, inorganic salts 0.05～5%, and all the other are sterilized water; The component of secondary medium is in described step (1) and (2): wheat bran 10～70%, inorganic salts 0.05～2%, all the other are sterilized water.
The access amount of aspergillus niger spore suspension is 0.1～1% of an one-level culture medium weight in the described step (1), and the access amount of primary seed solution is 0.1～10% of a secondary medium weight.
The access amount of saccharomycete spore suspension is 0.1～1% of an one-level culture medium weight in the described step (2), and the access amount of primary seed solution is 0.1～10% of a secondary medium weight.
Described inorganic salts are one or more in potassium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, the magnesium sulfate.
Adopt the pneumatic conveying drying method during described oven dry, EAT is 125～140 ℃, and leaving air temp is 40～60 ℃.
The present invention is raw material with the wheat bran, through the composite bacteria solid fermentation, utilize the cellulase of aspergillus niger secretion and the protease of saccharomycete secretion, and add lecithinase, decompose the fat in the seitan, obtain a kind of functional (high activity) wheat bran that contains the various active composition.The prepared functional wheat bran of the present invention has been removed the multiple ANFs in the plant protein material, be rich in high activity wheat-bran dietary fiber, highly active protein, use and produce little peptide and lactic acid simultaneously, being absorbed by body easily, is a kind of high-quality health product, and this product can improve the aggregate velocity of protein in body, fermenting aroma can promote to search for food, strengthen body's immunity, promote the absorption of body to mineral element, it is movable and improve the nutrition condition of body to regulate organism physiology.The present invention has realized the effective increment and the utilization of wheat bran when providing healthy food for people, social benefit and economy are all very remarkable.
The specific embodiment
Embodiment 1:
May further comprise the steps: (1) aspergillus niger obtains aspergillus niger spore after cultivating 65 hours at 28 ℃ of lower inclined planes; Wash aspergillus niger spore with SPSS and obtain the aspergillus niger spore suspension; The aspergillus niger spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of aspergillus niger spore suspension is 6% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 15%, potassium dihydrogen phosphate 3%, all the other are sterilized water, cultivated 26 hours down at 28 ℃, obtain the aspergillus niger primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 0.1% of a secondary medium weight, the component of secondary medium is: wheat bran 30%, potassium chloride 1.5%, all the other are sterilized water, cultivated 40 hours down at 28 ℃, obtain aspergillus niger secondary seed solution;
(2) saccharomycete obtains the saccharomycete spore, the slant medium that used slant medium is made for the wheat bran hydrolyzate after cultivating 60 hours at 28 ℃ of lower inclined planes; Wash the saccharomycete spore with SPSS and obtain saccharomycete spore suspension; Saccharomycete spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of saccharomycete spore suspension is 5.5% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 22%, potassium dihydrogen phosphate 3%, all the other are sterilized water, cultivated 24 hours down at 28 ℃, obtain the saccharomycete primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 6% of a secondary medium weight, the component of secondary medium is: wheat bran 70%, potassium dihydrogen phosphate 2%, all the other are sterilized water, cultivated 45 hours down at 28 ℃, obtain the saccharomycete secondary seed solution;
(3) aspergillus niger secondary seed solution that step (1) and (2) are obtained and saccharomycete secondary seed solution insert fermentation medium, the access amount of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 6% of a fermentation medium weight, the weight ratio of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 1: 4, the component of fermentation medium is: wheat bran 60%, potassium dihydrogen phosphate 1.5%, all the other are sterilized water, at 28 ℃ of bottom fermentations after 18 hours, add lecithinase, the mass concentration that adds the back lecithinase is 600ppm, keeping pH value is 7.5, cultivated 60 hours down at 28 ℃, oven dry obtains functional wheat bran then, adopt the pneumatic conveying drying method during oven dry, EAT is 135 ℃, and leaving air temp is 55 ℃.
Adopt bacterial classification to cultivate slant medium commonly used when the inclined-plane is cultivated in step (1) and the step (2).
Embodiment 2:
May further comprise the steps: (1) aspergillus niger obtains aspergillus niger spore, the slant medium that used slant medium is made for the wheat bran hydrolyzate after cultivating 72 hours at 20 ℃ of lower inclined planes; Wash aspergillus niger spore with SPSS and obtain the aspergillus niger spore suspension; The aspergillus niger spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of aspergillus niger spore suspension is 0.1% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 40%, potassium chloride 0.05%, all the other are sterilized water, cultivated 30 hours down at 22 ℃, obtain the aspergillus niger primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 0.1% of a secondary medium weight, the component of secondary medium is: wheat bran 70%, potassium chloride 0.05%, all the other are sterilized water, cultivated 50 hours down at 22 ℃, obtain aspergillus niger secondary seed solution;
(2) saccharomycete obtains the saccharomycete spore, the slant medium that used slant medium is made for the wheat bran hydrolyzate after cultivating 72 hours at 20 ℃ of lower inclined planes; Wash the saccharomycete spore with SPSS and obtain saccharomycete spore suspension; Saccharomycete spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of saccharomycete spore suspension is 0.1% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 40%, potassium chloride 5%, all the other are sterilized water, cultivated 30 hours down at 22 ℃, obtain the saccharomycete primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 0.1% of a secondary medium weight, the component of secondary medium is: wheat bran 70%, potassium chloride 2%, all the other are sterilized water, cultivated 50 hours down at 22 ℃, obtain the saccharomycete secondary seed solution;
(3) aspergillus niger secondary seed solution that step (1) and (2) are obtained and saccharomycete secondary seed solution insert fermentation medium, the access amount of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 0.1% of a fermentation medium weight, the weight ratio of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 1: 1, the component of fermentation medium is: wheat bran 70%, potassium chloride 2%, all the other are sterilized water, at 22 ℃ of bottom fermentations after 30 hours, add lecithinase, the mass concentration that adds the back lecithinase is 800ppm, keeping pH value is 6.0, cultivated 98 hours down at 22 ℃, oven dry obtains functional wheat bran then, adopt the pneumatic conveying drying method during oven dry, EAT is 125 ℃, and leaving air temp is 40 ℃.
The slant medium that wheat bran hydrolyzate in step (1) and the step (2) is made adopts following method preparation: the water that adds 4 times of its weight in wheat bran, smash pulping, the lecithinase that adds slurry weight 0.05%, 40 ℃ of following saccharification 4 hours, boil the centrifugal supernatant that gets after 20 minutes, every 100ml supernatant adding 1g agar.
Embodiment 3:
May further comprise the steps: (1) aspergillus niger obtains aspergillus niger spore, the slant medium that used slant medium is made for the wheat bran hydrolyzate after cultivating 40 hours at 30 ℃ of lower inclined planes; Wash aspergillus niger spore with SPSS and obtain the aspergillus niger spore suspension; The aspergillus niger spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of aspergillus niger spore suspension is 0.5% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 20%, sodium hydrogen phosphate 2%, all the other are sterilized water, cultivated 20 hours down at 30 ℃, obtain the aspergillus niger primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 5% of a secondary medium weight, the component of secondary medium is: wheat bran 40%, sodium hydrogen phosphate 1%, all the other are sterilized water, cultivated 30 hours down at 30 ℃, obtain aspergillus niger secondary seed solution;
(2) saccharomycete obtains the saccharomycete spore, the slant medium that used slant medium is made for the wheat bran hydrolyzate after cultivating 40 hours at 30 ℃ of lower inclined planes; Wash the saccharomycete spore with SPSS and obtain saccharomycete spore suspension; Saccharomycete spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of saccharomycete spore suspension is 0.5% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 20%, sodium hydrogen phosphate 2%, all the other are sterilized water, cultivated 20 hours down at 30 ℃, obtain the saccharomycete primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 5% of a secondary medium weight, the component of secondary medium is: wheat bran 40%, sodium hydrogen phosphate 1%, all the other are sterilized water, cultivated 30 hours down at 30 ℃, obtain the saccharomycete secondary seed solution;
(3) aspergillus niger secondary seed solution that step (1) and (2) are obtained and saccharomycete secondary seed solution insert fermentation medium, the access amount of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 5% of a fermentation medium weight, the weight ratio of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 1: 5, the component of fermentation medium is: wheat bran 35%, sodium hydrogen phosphate and potassium dihydrogen phosphate 1.5%, all the other are sterilized water, at 35 ℃ of bottom fermentations after 15 hours, add lecithinase, the mass concentration that adds the back lecithinase is 400ppm, keeping pH value is 7.0, cultivated 52 hours down at 32 ℃, oven dry obtains functional wheat bran then, adopt the pneumatic conveying drying method during oven dry, EAT is 130 ℃, and leaving air temp is 55 ℃.
The slant medium that wheat bran hydrolyzate in step (1) and the step (2) is made adopts following method preparation: the water that adds 8 times of its weight in wheat bran, smash pulping, the lecithinase that adds slurry weight 0.03%, 60 ℃ of following saccharification 3 hours, boil the centrifugal supernatant that gets after 30 minutes, every 100ml supernatant adding 1.5g agar.
Embodiment 4:
May further comprise the steps: (1) aspergillus niger obtains aspergillus niger spore, the slant medium that used slant medium is made for the wheat bran hydrolyzate after cultivating 24 hours at 40 ℃ of lower inclined planes; Wash aspergillus niger spore with SPSS and obtain the aspergillus niger spore suspension; The aspergillus niger spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of aspergillus niger spore suspension is 1% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 5%, magnesium sulfate 5%, all the other are sterilized water, cultivated 8 hours down at 40 ℃, obtain the aspergillus niger primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 10% of a secondary medium weight, the component of secondary medium is: wheat bran 10%, magnesium sulfate 0.05%, all the other are sterilized water, cultivated 10 hours down at 40 ℃, obtain aspergillus niger secondary seed solution;
(2) saccharomycete obtains the saccharomycete spore, the slant medium that used slant medium is made for the wheat bran hydrolyzate after cultivating 24 hours at 40 ℃ of lower inclined planes; Wash the saccharomycete spore with SPSS and obtain saccharomycete spore suspension; Saccharomycete spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, the access amount of saccharomycete spore suspension is 1% of an one-level culture medium weight, the component of one-level culture medium is: wheat bran 5%, magnesium sulfate 0.05%, all the other are sterilized water, cultivated 8 hours down at 40 ℃, obtain the saccharomycete primary seed solution; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, the access amount of primary seed solution is 10% of a secondary medium weight, the component of secondary medium is: wheat bran 10%, magnesium sulfate 0.05%, all the other are sterilized water, cultivated 10 hours down at 40 ℃, obtain the saccharomycete secondary seed solution;
(3) aspergillus niger secondary seed solution that step (1) and (2) are obtained and saccharomycete secondary seed solution insert fermentation medium, the access amount of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 10% of a fermentation medium weight, the weight ratio of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 1: 10, the component of fermentation medium is: wheat bran 10%, magnesium sulfate 0.05%, all the other are sterilized water, at 40 ℃ of bottom fermentations after 1 hour, add lecithinase, the mass concentration that adds the back lecithinase is 100ppm, keeping pH value is 8.0, cultivated 10 hours down at 40 ℃, oven dry obtains functional wheat bran then, adopt the pneumatic conveying drying method during oven dry, EAT is 140 ℃, and leaving air temp is 60 ℃.
The slant medium that wheat bran hydrolyzate in step (1) and the step (2) is made adopts following method preparation: the water that adds 10 times of its weight in wheat bran, smash pulping, the lecithinase that adds slurry weight 0.01%, 80 ℃ of following saccharification 1 hour, boil the centrifugal supernatant that gets after 40 minutes, every 100ml supernatant adding 2g agar.
The composition measurement of functional wheat bran:
Determining the protein quantity: micro-Kai Shi (Kjeldahl) nitriding, the sample and the concentrated sulfuric acid be warm altogether.Itrogenous organic substance promptly decomposes and produces ammonia (digestion), ammonia again with effect of sulfuric acid, become sulfate of ammoniac.Make it to decompose through highly basic alkalization and emit ammonia, borrow steam that ammonia is steamed to acid solution, the degree that is neutralized according to this acid solution can calculate the nitrogen content of sample.
Little peptide content is measured: GB/T6682 assay laboratory water specification and experimental technique, earlier soluble protein, free amino acid and ammonia in the sample are extracted in water, extracting section liquid is at 110 ℃, hydrolysis 24h becomes single amino acids in the 6mol/L hydrochloric acid, measure the total amino acid nitrogen content at the 570nm place through the ninhydrin chromogenic reaction, free amino acid nitrogen content direct with extract and that the ninhydrin chromogenic reaction is measured at the 570nm place is a blank, and water-soluble peptide content promptly is to multiply by conversion coefficient again after the total amino acid nitrogen content deducts the free amino acid nitrogen content.
Sample is ignored in the error that free ammonia brought that the acid hydrolysis process produces in this method.
The determination of fat: what use conventional methods is to use soxhlet extraction, behind the extraction grease, measures peroxide value with the grease that extracts again.
Content of cellulose is measured: reference to standard GB/T 601 chemical reagent titrimetries (capacity analysis) preparation of standard liquid, with concentration bronsted lowry acids and bases bronsted lowry accurately, disappear under given conditions and boil sample, remove DDGS with ethanol again, through the amount of high temperature sintering deduction mineral matter, institute's surplus is a crude fibre, it is not a definite chemical entities, just the summary composition of measuring under the condition of generally acknowledging mandatory provision wherein based on cellulose, also has a small amount of hemicellulose and lignin.
Measurement result sees the following form 1:
Albumen and fatty situation in the different embodiment products of table 1
Conclusion: wheat bran is obtaining functional wheat bran through after the fermenting and producing of the present invention, and the protein content in the functional wheat bran raises 100%, and little peptide content raises 30%, and fat content falls 30%, and content of cellulose falls 20%.Little peptide accounts for protein content=little peptide and accounts for the amount/protein content of total material.
Employed lecithinase can company of section be produced by the U.S. is outstanding among the present invention, and aspergillus niger and saccharomycete can directly be buied by market.
Claims (10)
1, the fermentation method for producing of functional wheat bran is characterized in that may further comprise the steps: (1) aspergillus niger is cultivated the back through the inclined-plane and is obtained aspergillus niger spore; Wash aspergillus niger spore with SPSS and obtain the aspergillus niger spore suspension; The aspergillus niger spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, cultivated 8～30 hours down, obtain the aspergillus niger primary seed solution at 22～40 ℃; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, cultivated 10～50 hours down, obtain aspergillus niger secondary seed solution at 22～40 ℃;
(2) saccharomycete is cultivated the back through the inclined-plane and is obtained the saccharomycete spore; Wash the saccharomycete spore with SPSS and obtain saccharomycete spore suspension; Saccharomycete spore suspension is inserted the one-level culture medium shake a bottle first order seed cultivation, cultivated 8～30 hours down, obtain the saccharomycete primary seed solution at 22～40 ℃; Primary seed solution is inserted secondary medium shake a bottle secondary cultivation, cultivated 10～50 hours down, obtain the saccharomycete secondary seed solution at 22～40 ℃;
(3) aspergillus niger secondary seed solution that step (1) and (2) are obtained and saccharomycete secondary seed solution insert the fermentation medium of being made by the materials of wheat wheat bran, at 22～40 ℃ of bottom fermentations after 1～30 hour, add lecithinase, the mass concentration that adds the back lecithinase is 100～800ppm, keeping pH value is 6.0～8.0, cultivated 10～98 hours down at 22～40 ℃, oven dry obtains functional wheat bran then.
2, the fermentation method for producing of functional wheat bran as claimed in claim 1 is characterized in that: the component of described fermentation medium is: wheat bran 10～70%, inorganic salts 0.05～2%, all the other are sterilized water.
3, the fermentation method for producing of functional wheat bran as claimed in claim 2, it is characterized in that: the access amount of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 0.1～10% of a fermentation medium weight in the described step (3), and the weight ratio of aspergillus niger secondary seed solution and saccharomycete secondary seed solution is 1: 1～10.
4, as the fermentation method for producing of each described functional wheat bran of claim 1 to 3, it is characterized in that: cultivated 24～72 hours down at 20～40 ℃ when the inclined-plane is cultivated in described step (1) and the step (2).
5, the fermentation method for producing of functional wheat bran as claimed in claim 4, it is characterized in that: the slant medium that the culture medium that adopts when the inclined-plane is cultivated in described step (1) and the step (2) is made for the wheat bran hydrolyzate, this culture medium adopts following method preparation: the water that adds 4～10 times of its weight in wheat bran, smash pulping, the lecithinase that adds slurry weight 0.01～0.05%, 40～80 ℃ of following saccharification 1～4 hour, boil the centrifugal supernatant that gets after 20～40 minutes, every 100ml supernatant adding 1～2g agar.
6, the fermentation method for producing of functional wheat bran as claimed in claim 4 is characterized in that: the component of one-level culture medium is in described step (1) and (2): wheat bran 5～40%, inorganic salts 0.05～5%, and all the other are sterilized water; The component of secondary medium is in described step (1) and (2): wheat bran 10～70%, inorganic salts 0.05～2%, all the other are sterilized water.
7, the fermentation method for producing of functional wheat bran as claimed in claim 6, it is characterized in that: the access amount of aspergillus niger spore suspension is 0.1～1% of an one-level culture medium weight in the described step (1), and the access amount of primary seed solution is 0.1～10% of a secondary medium weight.
8, the fermentation method for producing of functional wheat bran as claimed in claim 6, it is characterized in that: the access amount of saccharomycete spore suspension is 0.1～1% of an one-level culture medium weight in the described step (2), and the access amount of primary seed solution is 0.1～10% of a secondary medium weight.
9, the fermentation method for producing of functional wheat bran as claimed in claim 6 is characterized in that: described inorganic salts are one or more in potassium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, the magnesium sulfate.
10, the fermentation method for producing of functional wheat bran as claimed in claim 6 is characterized in that: adopt the pneumatic conveying drying method during described oven dry, EAT is 125～140 ℃, and leaving air temp is 40～60 ℃.
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| CN101884783A (en) * | 2010-07-08 | 2010-11-17 | 辽宁大学 | Wheat bran polypeptide-containing anti-fatigue traditional Chinese medicine and preparation method thereof |
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| CN1565228A (en) * | 2003-07-03 | 2005-01-19 | 延边高丽家庭生活服务咨询有限公司 | Straw fodder leaven |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101884783A (en) * | 2010-07-08 | 2010-11-17 | 辽宁大学 | Wheat bran polypeptide-containing anti-fatigue traditional Chinese medicine and preparation method thereof |
| CN101884783B (en) * | 2010-07-08 | 2013-03-06 | 辽宁大学 | Wheat bran polypeptide-containing anti-fatigue traditional Chinese medicine and preparation method thereof |
| CN106819360A (en) * | 2017-01-04 | 2017-06-13 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of edible fungi residue feed additive and preparation method and application |
| CN108191166A (en) * | 2017-08-01 | 2018-06-22 | 王建玲 | Recycling device and treatment method of thick white water for papermaking |
| CN108191166B (en) * | 2017-08-01 | 2020-11-13 | 绍兴凯达纺织装饰品有限公司 | Recycling device and treatment method of thick white water for papermaking |
| CN109123370A (en) * | 2018-09-04 | 2019-01-04 | 江苏科技大学 | A kind of fermented type wheat bran dietary fiber chewable tablets and its preparation method and application |
| CN109744366A (en) * | 2019-03-21 | 2019-05-14 | 佛山奇诺福饲料科技有限公司 | A kind of feed addictive and its application method |
| CN110157627A (en) * | 2019-06-04 | 2019-08-23 | 巢湖学院 | A kind of preparation method of wheat bran Monascus fermentation broth |
| CN110946211A (en) * | 2019-11-12 | 2020-04-03 | 福建御研农业科技有限公司 | Fermented wheat bran and preparation method and application thereof |
| CN110804574A (en) * | 2019-12-06 | 2020-02-18 | 湖北华扬科技发展有限公司 | High-concentration bacillus coagulans liquid fermentation method |
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