CN1565228A

CN1565228A - Straw fodder leaven

Info

Publication number: CN1565228A
Application number: CNA031482872A
Authority: CN
Inventors: 李春烈
Original assignee: Yanbian Korea Domesticity Service And Consultation Co ltd
Priority date: 2003-07-03
Filing date: 2003-07-03
Publication date: 2005-01-19

Abstract

The straw fodder leaven comprises leaven A and leaven B, wherein the leaven A employs bacterial A, the leaven B employs B1, B2 and B3 bacteria, the four bacteria are cultured and inoculated on agar medium at the temperature of 30 deg. C to 37 deg. C for 2-3 days to obtain leaven A and leaven B.

Description

The producting stalk fodder starter

A kind of A starter and use B with the A strain preparation ₁Bacterium, B ₂Bacterium and B ₃The producting stalk fodder starter that the B starter of bacterium preparation is used.

1, technical field

Patent of the present invention belongs to for straw roughage fermentation feed fermentation agent.

2, background technology

If uplift the people's living standard with quality of life, must greatly develop livestock industry, need a large amount of range resources.Yet China's useful grassland area is few at present, adds the grazing management of long-term lacking science, and a large amount of grassland will be improved, and high quality forage is under-supply; Past is made feed with grain and feeds livestock and poultry, makes feed cost height, waste grain, the situation that livestock and poultry and people fight for grain occurs.Will develop producting stalk fodder now and save food, replenish the herbage deficiency, be to solve people and animals to strive the grain situation, for the livestock industry fast development provides more wide approach.China's straw aboundresources, special three provinces in the northeast of China straw resource is preponderated, and most of peasants and herdsmen of past directly give straw roughage and feed, and the result is not high because of its nutritive value, and the cattle and sheep livestock and poultry are not liked to eat, and effect is not so good; Found afterwards producting stalk fodder is fermented the back to feeding, livestock and poultry are liked to eat, and can worry because of can't find suitable starter.

3, the purpose of patent of the present invention

The purpose of patent of the present invention is to provide that a kind of technology is simple, low price, effective producting stalk fodder starter, straw is fully fermented, the Mierocrystalline cellulose that reaches in its straw is broken down into materials such as the digestible protein of livestock and poultry, improve nutritive value, thereby the appetite of increase livestock and poultry, improve the resistibility of livestock and poultry to various diseases.

4, the enforcement of patent of invention

The bacterial classification that patent of the present invention adopted is asked for by Institute of Microorganism, Academia Sinica, has respectively on the A starter with the B that uses on A starter kind yeast saccharomyces cerevisiae and the B starter in the B bacterium starter kind ₁Bacterium aspergillus niger, B ₂Yeast saccharomyces cerevisiae, B ₃The bacterium lactobacillus.It is husky Blaw (sabouraud) slant medium that each spawn culture is respectively A bacterial classification mother culture media with mother culture media, the B of B starter ₁The female breeding base of bacterial classification is husky Blaw plate culture medium, B ₂The bacterial classification mother culture media is husky Blaw plate culture medium, B ₂The bacterial classification mother culture media is for looking into (czapek-dox) family name substratum, B ₃The bacterial classification mother culture media is the plain agar substratum.The making processes of two kinds of starters is respectively, the A bacterial classification inoculation is contained in nothing (its loading amount is respectively 250 milliliters, 500 milliliters, 1000 milliliters, 10000 milliliters) places 30 ℃ of incubators or culturing room on the husky Blaw substratum of agar, 2-3 hour shake 5 minutes or substratum placed cultivate 48～72 hours on the shaker at interval, make its bacterium amount reach 20～300,000/milliliter, under aseptic condition, be sub-packed in airtight getting final product in the aseptic plastic bottle then; The B of B starter ₁Bacterial classification places the culture of cultivating on the husky Blaw plate culture medium to be inoculated on the wheat bran plate substratum and cultivated 72 hours, this culture is inoculated in sterilized wheat bran (or volume ratio is on 100: 1 the corn face), thorough mixing is placed on trogue, cultivates 3～4 days in 30 ℃ of room temperatures.Deliver to then do after the kiln drying standby, B ₂Microbial strain culture be inoculated on the Sha Bufang liquid nutrient medium that does not contain agar (its loading amount is respectively 250 milliliters, 500 milliliters, 1000 milliliters, 10000 milliliters) in 30 ℃ of incubators or culturing room inner room every 2～3 hours shakes 5 minutes, or substratum on shaker, cultivate did in 24～72 hours standby, B ₃The culture that bacterial classification is cultivated on the plain agar substratum is inoculated in to cultivate in the incubator of putting 37 ℃ on the broth culture to be done standby in 24～38 hours; More than in 3 kinds of spares with B ₁: B ₂=50kg: take 1000 milliliters of B after 1000 milliliters the mixed ₃Spares is sprayed on B with atomizer ₁On the bacterium culture, fully stir the back with agitator thereafter and in 30 ℃ of greenhouses, carry out drying, be ground into the packing of B starter with pulverizer then and get final product.The patent user of the present invention back of taking away imposes on the collocation of A starter and B starter in the straw roughage that has cut on probationly by working instructions, effect is quite desirable, obtains user's favorable comment and commendation.

Claims

1, a kind of feed fermentation agent is applied on the straw roughage by A starter and the collocation of B starter, it is characterized in that:

1. the bacterial classification that the adopts A starter manufacturing of respectively doing for oneself is a yeast saccharomyces cerevisiae with the A bacterial classification, and the manufacturing of B starter is that aspergillus niger, B2 bacterium are that yeast saccharomyces cerevisiae, B3 bacterium are lactobacillus with the B1 bacterial classification;

2. starter kind substratum respectively do for oneself A starter kind be on husky Blaw (sabouraud) family name agar slant culture-medium, B1 starter kind be on the husky cloth Lloyd's plate culture medium, B2 starter kind be look on (czapek-dox) family name substratum, B3 starter kind is to cultivate on the plain agar substratum;

3. the making processes of two kinds of starters is respectively and the A bacterial classification inoculation is contained in nothing (its loading amount is respectively 250 milliliters, 500 milliliters, 1000 milliliters, 10000 milliliters) places 30 ℃ of incubators or culturing room on the husky Blaw substratum of agar, 2-3 hour shake 5 minutes or substratum placed cultivate 48～72 hours on the shaker at interval, make its bacterium amount reach 20～300,000/milliliter, under aseptic condition, be sub-packed in airtight getting final product in the aseptic plastic bottle then; The B of B starter ₁Bacterial classification places the culture of cultivating on the husky Blaw plate culture medium to be inoculated on the wheat bran plate substratum and cultivated 72 hours, this culture is inoculated in sterilized wheat bran (or volume ratio is on 100: 1 the corn face), thorough mixing is placed on trogue, cultivates 3～4 days in 30 ℃ of room temperatures.Deliver to then do after the kiln drying standby, B ₂Microbial strain culture be inoculated on the Sha Bufang liquid nutrient medium that does not contain agar (its loading amount is respectively 250 milliliters, 500 milliliters, 1000 milliliters, 10000 milliliters) in 30 ℃ of incubators or culturing room inner room every 2～3 hours shakes 5 minutes, or substratum on shaker, cultivate did in 24～72 hours standby, B ₃The culture that bacterial classification is cultivated on the plain agar substratum is inoculated in to cultivate in the incubator of putting 37 ℃ on the broth culture to be done standby in 24～38 hours; More than in 3 kinds of spares with B ₁: B ₂=50kg: take 1000 milliliters of B after 1000 milliliters the mixed ₃Spares is sprayed on B with atomizer ₁On the bacterium culture, fully stir the back with agitator thereafter and in 30 ℃ of greenhouses, carry out drying, be ground into the packing of B starter with pulverizer then and get final product.