CN101495499B - 编码类异戊烯修饰酶的多核苷酸和其使用方法 - Google Patents

编码类异戊烯修饰酶的多核苷酸和其使用方法 Download PDF

Info

Publication number
CN101495499B
CN101495499B CN2006800246664A CN200680024666A CN101495499B CN 101495499 B CN101495499 B CN 101495499B CN 2006800246664 A CN2006800246664 A CN 2006800246664A CN 200680024666 A CN200680024666 A CN 200680024666A CN 101495499 B CN101495499 B CN 101495499B
Authority
CN
China
Prior art keywords
nucleic acid
host cell
nucleotide sequence
isopentenoid
encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN2006800246664A
Other languages
English (en)
Chinese (zh)
Other versions
CN101495499A (zh
Inventor
D·-K·罗
K·纽曼
E·M·帕若蒂斯
J·D·基斯林
M·奥莱特
R·伊切斯
K·霍
T·哈姆
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California San Diego UCSD
Original Assignee
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD
Priority to CN201710271576.5A priority Critical patent/CN106939323B/zh
Priority to CN201610412819.8A priority patent/CN105950667A/zh
Priority to CN201310335874.8A priority patent/CN103667370B/zh
Publication of CN101495499A publication Critical patent/CN101495499A/zh
Application granted granted Critical
Publication of CN101495499B publication Critical patent/CN101495499B/zh
Anticipated expiration legal-status Critical
Active legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN2006800246664A 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法 Active CN101495499B (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201710271576.5A CN106939323B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201610412819.8A CN105950667A (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201310335874.8A CN103667370B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US69706705P 2005-07-05 2005-07-05
US60/697,067 2005-07-05
PCT/US2006/025572 WO2007005604A2 (en) 2005-07-05 2006-06-29 Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof

Related Child Applications (3)

Application Number Title Priority Date Filing Date
CN201310335874.8A Division CN103667370B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201610412819.8A Division CN105950667A (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201710271576.5A Division CN106939323B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法

Publications (2)

Publication Number Publication Date
CN101495499A CN101495499A (zh) 2009-07-29
CN101495499B true CN101495499B (zh) 2013-09-04

Family

ID=37605033

Family Applications (4)

Application Number Title Priority Date Filing Date
CN2006800246664A Active CN101495499B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201710271576.5A Active CN106939323B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201610412819.8A Pending CN105950667A (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201310335874.8A Active CN103667370B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法

Family Applications After (3)

Application Number Title Priority Date Filing Date
CN201710271576.5A Active CN106939323B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201610412819.8A Pending CN105950667A (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法
CN201310335874.8A Active CN103667370B (zh) 2005-07-05 2006-06-29 编码类异戊烯修饰酶的多核苷酸和其使用方法

Country Status (18)

Country Link
US (2) US8163980B2 (enExample)
EP (2) EP2489672B1 (enExample)
JP (1) JP2009504138A (enExample)
KR (2) KR101444090B1 (enExample)
CN (4) CN101495499B (enExample)
AP (1) AP3827A (enExample)
BR (1) BRPI0612411B8 (enExample)
CA (1) CA2613469C (enExample)
DK (1) DK1919514T3 (enExample)
ES (2) ES2386554T3 (enExample)
IL (1) IL188376A (enExample)
MX (1) MX2008000200A (enExample)
PL (1) PL1919514T3 (enExample)
PT (1) PT1919514E (enExample)
SG (1) SG176459A1 (enExample)
SI (1) SI1919514T1 (enExample)
WO (1) WO2007005604A2 (enExample)
ZA (1) ZA200800889B (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2386359T3 (es) * 2004-07-27 2012-08-17 The Regents Of The University Of California Células huésped modificadas genéticamente y uso de las mismas para producir compuestos isoprenoides
DK1919514T3 (da) * 2005-07-05 2012-08-20 Univ California Polynucleotider kodende for isoprenoid-modificerende enzymer og fremgangsmåder til anvendelse deraf
CA2651747C (en) 2006-05-26 2017-10-24 Amyris Biotechnologies, Inc. Production of isoprenoids
DK3020799T3 (da) 2006-09-26 2020-09-21 Amyris Biotechnologies Inc Fremstilling af isoprenoider og isoprenoid-prækursorer
CA2692893C (en) 2007-06-01 2015-06-23 The Scripps Research Institute High throughput screening of genetically modified photosynthetic organisms
NZ583620A (en) 2007-09-11 2012-08-31 Sapphire Energy Inc Methods of producing organic fuel products with photosynthetic organisms
EP2444415A1 (en) * 2010-10-20 2012-04-25 Genoplante-Valor 1-Deoxy-D-xylulose 5-phosphate synthase alleles responsible for enhanced terpene biosynthesis
MX352318B (es) * 2011-11-01 2017-11-21 Firmenich & Cie Citocromo p450 y su uso para oxidacion enzimatica de terpenos.
JP5827601B2 (ja) * 2012-07-04 2015-12-02 住友ゴム工業株式会社 特定の蛋白質の発現を光応答転写因子により調整する方法、光応答転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法
CA2880929A1 (en) * 2012-09-07 2014-03-13 Sanofi Fusion proteins for treating a metabolic syndrome
PL224377B1 (pl) * 2012-11-02 2016-12-30 Inst Biochemii I Biofizyki Polskiej Akademii Nauk Szczep Trichoderma atroviride, sposób otrzymywania i zastosowanie szczepu Trichoderma atroviride jako środka biobójczego
CA2924541A1 (en) * 2013-09-19 2015-03-26 Firmenich Sa Method for producing fragrant alcohols
JP6475336B2 (ja) * 2014-08-21 2019-02-27 チュン−アン ユニヴァーシティ インダストリー−アカデミー コーポレーション ファウンデーションＣｈｕｎｇ−Ａｎｇ Ｕｎｉｖｅｒｓｉｔｙ Ｉｎｄｕｓｔｒｙ−Ａｃａｄｅｍｙ Ｃｏｏｐｅｒａｔｉｏｎ Ｆｏｕｎｄａｔｉｏｎ ｒＤＮＡ ＮＴＳ基盤の遺伝子多重挿入カセットセット、及びそれを用いたＧＲＡＳ等級の組換え酵母菌株
EP3283615A4 (en) * 2015-04-15 2018-09-05 William Marsh Rice University Iterative platform for the synthesis of alpha functionalized products
US11697830B2 (en) * 2015-04-15 2023-07-11 Ramon Gonzalez Iterative platform for the synthesis of alpha functionalized products
CN105177007B (zh) * 2015-10-30 2017-11-10 上海交通大学 一种调控基因在非分泌型腺毛中表达的启动子及其应用
CN105274109B (zh) * 2015-10-30 2017-12-29 上海交通大学 一种调控基因在非分泌型腺毛中表达的启动子及其应用
CN105483236A (zh) * 2015-12-26 2016-04-13 吉林省农业科学院 转基因植物中cry1C基因的LAMP检测引物组、试剂盒及检测方法
CN105567685B (zh) * 2016-01-18 2018-07-13 上海交通大学 AaPDR3基因启动子及其应用
CA3036968A1 (en) 2016-09-20 2018-03-29 22Nd Century Limited, Llc Trichome specific promoters for the manipulation of cannabinoids and other compounds in glandular trichomes
WO2018111194A1 (en) * 2016-12-16 2018-06-21 Temasek Life Sciences Laboratory Limited Targets for improving terpene production in rhodosporidium toruloides
AU2018239671B2 (en) 2017-03-24 2024-08-15 Trait Biosciences, Inc. High level In vivo biosynthesis and isolation of water-soluble cannabinoids in plant systems
US11946059B2 (en) 2017-07-11 2024-04-02 Trait Biosciences, Inc. In vivo generation of water-soluble cannabinoids in plant cell suspension cultures
EP3730145A1 (en) 2017-07-11 2020-10-28 Trait Biosciences, Inc. Generation of water-soluble cannabinoid compounds in yeast and plant cell suspension cultures and compositions of matter
US11905543B2 (en) 2017-07-11 2024-02-20 Trait Biosceinces, Inc. In vivo generation of water-soluble acetylated cannabinoid glycoside compounds in plant cell suspension cultures
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
MX2022009071A (es) * 2020-01-23 2022-08-15 Amyris Inc Variantes de amorfa-4, 11-dieno 12-monooxigenasa y usos de las mismas.
CN111621508B (zh) * 2020-06-11 2022-07-01 云南中烟工业有限责任公司 烟草萜类合成酶NtTPS7基因及其载体与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0982404A1 (en) * 1998-08-27 2000-03-01 Wallaart, Thorvald Eelco DNA encoding amorpha-4,11-diene synthase

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
EP0640136B1 (en) * 1992-03-27 2003-05-07 INTERNATIONAL FLOWER DEVELOPMENTS Pty. Ltd. Genetic sequences encoding flavonoid pathway enzymes with flavonoid 3'-hydroxylase activity and uses thereof
FR2689905B1 (fr) * 1992-04-13 1995-07-13 Orsan Procede de clonage d'une sequence d'adn codant pour une nadph cytochrome p450 reductase et sequences d'adn codant pour une nadph-cytochrome p450 reductase, notamment de plantes.
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5545818A (en) 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
US5545817A (en) 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
DE69526377T2 (de) 1994-12-09 2002-11-07 Microscience Ltd Virulenzgene in der VGC2-Region von Salmonella
AR011182A1 (es) * 1997-03-07 2000-08-02 Syngenta Participations Ag Molecula de adn, monooxigenasa de citocromo p450, codificada por dicha molecula, un metodo para el aislamiento de una molecula de adnc, un metodo parala produccion de una monooxigenasa de citocromo p450 recombinante purificada, una planta transgenica, un metodo para obtener dicha planta, el uso de d
US6900012B1 (en) 1997-06-03 2005-05-31 The University Of Chicago Plant artificial chromosome compositions and methods
US6083731A (en) * 1997-06-24 2000-07-04 Washington State University Research Foundation Recombinant materials and methods for the production of limonene hydroxylases
US6080849A (en) 1997-09-10 2000-06-27 Vion Pharmaceuticals, Inc. Genetically modified tumor-targeted bacteria with reduced virulence
US6645747B1 (en) 1999-09-21 2003-11-11 E. I. Du Pont De Nemours And Company Cis-prenyltransferases from plants
US6369212B1 (en) 2000-02-07 2002-04-09 Korea Kumbo Petrochemical Co., Ltd. Cytochrome P450 gene highly expressed in the incompatible interaction
EP1354955A4 (en) * 2000-12-28 2005-01-05 Toyota Motor Co Ltd PROCESS FOR PREPARING PRENYL ALCOHOL
ATE513046T1 (de) * 2001-03-09 2011-07-15 Univ Kentucky Res Found Cytochrom p450s und verwendungen davon
GB0105924D0 (en) 2001-03-09 2001-04-25 Microscience Ltd Promoter
AUPR446701A0 (en) * 2001-04-18 2001-05-17 Gene Stream Pty Ltd Transgenic mammals for pharmacological and toxicological studies
CN1589327A (zh) 2001-09-17 2005-03-02 植物研究国际公司 用于生物转化的植物酶
US20040162420A1 (en) 2002-10-16 2004-08-19 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
US6733996B2 (en) 2002-08-26 2004-05-11 Trustees Of Dartmouth College Methods for regulating gene expression using light
DK1919514T3 (da) * 2005-07-05 2012-08-20 Univ California Polynucleotider kodende for isoprenoid-modificerende enzymer og fremgangsmåder til anvendelse deraf
CA2630305A1 (en) * 2005-10-24 2007-05-03 National Research Council Of Canada A novel gene encoding amorphadiene hydroxylase and a process to produce artemisinic alcohol therewith

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0982404A1 (en) * 1998-08-27 2000-03-01 Wallaart, Thorvald Eelco DNA encoding amorpha-4,11-diene synthase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Yung-Jin Chang等.Amorpha-4,11-diene Synthase of Artemisia annua: cDNA Isolation and Bacterial Expression of a Terpene Synthase Involved in Artemisinin Biosynthesis.《Archives of Biochemistry and Biophysics》.2000,第383卷(第2期),178-184页. *
Yung-JinChang等.Amorpha-4 11-diene Synthase of Artemisia annua: cDNA Isolation and Bacterial Expression of a Terpene Synthase Involved in Artemisinin Biosynthesis.《Archives of Biochemistry and Biophysics》.2000
罗永明 等.植物萜类化合物的生物合成途径及其关键酶的研究进展.《江西中医学院学报》.2003,第15卷(第1期),45-51页. *

Also Published As

Publication number Publication date
EP2489672A2 (en) 2012-08-22
WO2007005604A2 (en) 2007-01-11
SG176459A1 (en) 2011-12-29
CN103667370B (zh) 2016-08-10
CN103667370A (zh) 2014-03-26
EP1919514B1 (en) 2012-05-16
CN106939323A (zh) 2017-07-11
KR20130054468A (ko) 2013-05-24
EP1919514A2 (en) 2008-05-14
ES2621657T3 (es) 2017-07-04
EP1919514A4 (en) 2009-12-02
PL1919514T3 (pl) 2012-10-31
ZA200800889B (en) 2009-09-30
SI1919514T1 (sl) 2012-08-31
BRPI0612411B8 (pt) 2021-05-25
BRPI0612411B1 (pt) 2020-05-05
MX2008000200A (es) 2008-04-07
US8759632B2 (en) 2014-06-24
US20120288905A1 (en) 2012-11-15
CN105950667A (zh) 2016-09-21
KR101444090B1 (ko) 2014-09-26
PT1919514E (pt) 2012-08-16
AP3827A (en) 2016-09-30
CN106939323B (zh) 2021-07-13
JP2009504138A (ja) 2009-02-05
ES2386554T3 (es) 2012-08-22
CA2613469A1 (en) 2007-01-11
AP2013006962A0 (en) 2013-07-31
IL188376A0 (en) 2008-04-13
EP2489672A3 (en) 2013-01-09
CN101495499A (zh) 2009-07-29
KR20080031054A (ko) 2008-04-07
US20100218283A1 (en) 2010-08-26
DK1919514T3 (da) 2012-08-20
KR101487304B1 (ko) 2015-01-28
WO2007005604A3 (en) 2008-12-31
BRPI0612411A2 (pt) 2010-11-09
IL188376A (en) 2012-12-31
BRPI0612411A8 (pt) 2017-12-26
US8163980B2 (en) 2012-04-24
CA2613469C (en) 2014-08-12
HK1134495A1 (en) 2010-04-30
EP2489672B1 (en) 2017-01-18

Similar Documents

Publication Publication Date Title
CN103667370B (zh) 编码类异戊烯修饰酶的多核苷酸和其使用方法
US8097438B2 (en) Nucleic acids encoding modified cytochrome P450 enzymes and methods of use thereof
CN103906834B (zh) 细胞色素p450及其在萜的酶促氧化中的用途
US20080233623A1 (en) Genetically modified host cells for increased p450 activity levels and methods of use thereof
US20070141574A1 (en) Method for identifying a biosynthetic pathway gene product
CN101283089A (zh) 修饰细胞色素p450酶的编码核酸和其应用方法
Misra et al. Characterization of cytochrome P450 monooxygenases isolated from trichome enriched fraction of Artemisia annua L. leaf
US20080187983A1 (en) Artemisinic epoxide and methods for producing same
HK1234443B (zh) 编码类异戊烯修饰酶的多核苷酸和其使用方法
HK1234443A1 (en) Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof
HK1134495B (en) Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof
HK1125132A (en) Nucleic acids encoding modified cytochrome p450 enzymes and methods of use thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1134495

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1134495

Country of ref document: HK