CA2613469C - Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof - Google Patents

Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof Download PDF

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Publication number
CA2613469C
CA2613469C CA2613469A CA2613469A CA2613469C CA 2613469 C CA2613469 C CA 2613469C CA 2613469 A CA2613469 A CA 2613469A CA 2613469 A CA2613469 A CA 2613469A CA 2613469 C CA2613469 C CA 2613469C
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Prior art keywords
isoprenoid
nucleotide sequence
nucleic acid
host cell
subject
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CA2613469A
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English (en)
French (fr)
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CA2613469A1 (en
Inventor
Karyn Newman
Eric M. Paradise
Jay D. Keasling
Mario Ouellet
Rachel Eachus
Kimberly Ho
Timothy Ham
Dae-Kyun Ro
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University of California San Diego UCSD
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University of California San Diego UCSD
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CA2613469A 2005-07-05 2006-06-29 Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof Active CA2613469C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US69706705P 2005-07-05 2005-07-05
US60/697,067 2005-07-05
PCT/US2006/025572 WO2007005604A2 (en) 2005-07-05 2006-06-29 Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof

Publications (2)

Publication Number Publication Date
CA2613469A1 CA2613469A1 (en) 2007-01-11
CA2613469C true CA2613469C (en) 2014-08-12

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CA2613469A Active CA2613469C (en) 2005-07-05 2006-06-29 Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof

Country Status (18)

Country Link
US (2) US8163980B2 (enExample)
EP (2) EP2489672B1 (enExample)
JP (1) JP2009504138A (enExample)
KR (2) KR101444090B1 (enExample)
CN (4) CN101495499B (enExample)
AP (1) AP3827A (enExample)
BR (1) BRPI0612411B8 (enExample)
CA (1) CA2613469C (enExample)
DK (1) DK1919514T3 (enExample)
ES (2) ES2386554T3 (enExample)
IL (1) IL188376A (enExample)
MX (1) MX2008000200A (enExample)
PL (1) PL1919514T3 (enExample)
PT (1) PT1919514E (enExample)
SG (1) SG176459A1 (enExample)
SI (1) SI1919514T1 (enExample)
WO (1) WO2007005604A2 (enExample)
ZA (1) ZA200800889B (enExample)

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JP5827601B2 (ja) * 2012-07-04 2015-12-02 住友ゴム工業株式会社 特定の蛋白質の発現を光応答転写因子により調整する方法、光応答転写因子をコードする遺伝子が導入されたイソプレノイド産生植物、及び該イソプレノイド産生植物を用いたポリイソプレノイドの製造方法
CA2880929A1 (en) * 2012-09-07 2014-03-13 Sanofi Fusion proteins for treating a metabolic syndrome
PL224377B1 (pl) * 2012-11-02 2016-12-30 Inst Biochemii I Biofizyki Polskiej Akademii Nauk Szczep Trichoderma atroviride, sposób otrzymywania i zastosowanie szczepu Trichoderma atroviride jako środka biobójczego
CA2924541A1 (en) * 2013-09-19 2015-03-26 Firmenich Sa Method for producing fragrant alcohols
JP6475336B2 (ja) * 2014-08-21 2019-02-27 チュン−アン ユニヴァーシティ インダストリー−アカデミー コーポレーション ファウンデーションＣｈｕｎｇ−Ａｎｇ Ｕｎｉｖｅｒｓｉｔｙ Ｉｎｄｕｓｔｒｙ−Ａｃａｄｅｍｙ Ｃｏｏｐｅｒａｔｉｏｎ Ｆｏｕｎｄａｔｉｏｎ ｒＤＮＡ ＮＴＳ基盤の遺伝子多重挿入カセットセット、及びそれを用いたＧＲＡＳ等級の組換え酵母菌株
EP3283615A4 (en) * 2015-04-15 2018-09-05 William Marsh Rice University Iterative platform for the synthesis of alpha functionalized products
US11697830B2 (en) * 2015-04-15 2023-07-11 Ramon Gonzalez Iterative platform for the synthesis of alpha functionalized products
CN105177007B (zh) * 2015-10-30 2017-11-10 上海交通大学 一种调控基因在非分泌型腺毛中表达的启动子及其应用
CN105274109B (zh) * 2015-10-30 2017-12-29 上海交通大学 一种调控基因在非分泌型腺毛中表达的启动子及其应用
CN105483236A (zh) * 2015-12-26 2016-04-13 吉林省农业科学院 转基因植物中cry1C基因的LAMP检测引物组、试剂盒及检测方法
CN105567685B (zh) * 2016-01-18 2018-07-13 上海交通大学 AaPDR3基因启动子及其应用
CA3036968A1 (en) 2016-09-20 2018-03-29 22Nd Century Limited, Llc Trichome specific promoters for the manipulation of cannabinoids and other compounds in glandular trichomes
WO2018111194A1 (en) * 2016-12-16 2018-06-21 Temasek Life Sciences Laboratory Limited Targets for improving terpene production in rhodosporidium toruloides
AU2018239671B2 (en) 2017-03-24 2024-08-15 Trait Biosciences, Inc. High level In vivo biosynthesis and isolation of water-soluble cannabinoids in plant systems
US11946059B2 (en) 2017-07-11 2024-04-02 Trait Biosciences, Inc. In vivo generation of water-soluble cannabinoids in plant cell suspension cultures
EP3730145A1 (en) 2017-07-11 2020-10-28 Trait Biosciences, Inc. Generation of water-soluble cannabinoid compounds in yeast and plant cell suspension cultures and compositions of matter
US11905543B2 (en) 2017-07-11 2024-02-20 Trait Biosceinces, Inc. In vivo generation of water-soluble acetylated cannabinoid glycoside compounds in plant cell suspension cultures
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
MX2022009071A (es) * 2020-01-23 2022-08-15 Amyris Inc Variantes de amorfa-4, 11-dieno 12-monooxigenasa y usos de las mismas.
CN111621508B (zh) * 2020-06-11 2022-07-01 云南中烟工业有限责任公司 烟草萜类合成酶NtTPS7基因及其载体与应用

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Also Published As

Publication number Publication date
EP2489672A2 (en) 2012-08-22
WO2007005604A2 (en) 2007-01-11
SG176459A1 (en) 2011-12-29
CN103667370B (zh) 2016-08-10
CN103667370A (zh) 2014-03-26
EP1919514B1 (en) 2012-05-16
CN106939323A (zh) 2017-07-11
KR20130054468A (ko) 2013-05-24
EP1919514A2 (en) 2008-05-14
ES2621657T3 (es) 2017-07-04
EP1919514A4 (en) 2009-12-02
PL1919514T3 (pl) 2012-10-31
ZA200800889B (en) 2009-09-30
SI1919514T1 (sl) 2012-08-31
BRPI0612411B8 (pt) 2021-05-25
BRPI0612411B1 (pt) 2020-05-05
MX2008000200A (es) 2008-04-07
US8759632B2 (en) 2014-06-24
US20120288905A1 (en) 2012-11-15
CN105950667A (zh) 2016-09-21
CN101495499B (zh) 2013-09-04
KR101444090B1 (ko) 2014-09-26
PT1919514E (pt) 2012-08-16
AP3827A (en) 2016-09-30
CN106939323B (zh) 2021-07-13
JP2009504138A (ja) 2009-02-05
ES2386554T3 (es) 2012-08-22
CA2613469A1 (en) 2007-01-11
AP2013006962A0 (en) 2013-07-31
IL188376A0 (en) 2008-04-13
EP2489672A3 (en) 2013-01-09
CN101495499A (zh) 2009-07-29
KR20080031054A (ko) 2008-04-07
US20100218283A1 (en) 2010-08-26
DK1919514T3 (da) 2012-08-20
KR101487304B1 (ko) 2015-01-28
WO2007005604A3 (en) 2008-12-31
BRPI0612411A2 (pt) 2010-11-09
IL188376A (en) 2012-12-31
BRPI0612411A8 (pt) 2017-12-26
US8163980B2 (en) 2012-04-24
HK1134495A1 (en) 2010-04-30
EP2489672B1 (en) 2017-01-18

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