CN101475882A - Cleanser containing microorganism and preparation thereof - Google Patents
Cleanser containing microorganism and preparation thereof Download PDFInfo
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- CN101475882A CN101475882A CNA2009100956758A CN200910095675A CN101475882A CN 101475882 A CN101475882 A CN 101475882A CN A2009100956758 A CNA2009100956758 A CN A2009100956758A CN 200910095675 A CN200910095675 A CN 200910095675A CN 101475882 A CN101475882 A CN 101475882A
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Abstract
The invention discloses a detergent containing microorganisms and a preparation method thereof. The detergent mainly comprises solute, a solvent and bacillus, wherein the solute mainly comprises 55 to 70 weight percent of alkyl phenol polyethenoxy ether-10, 29 to 44.5 weight percent of tristearin and 0.5 to 1 weight percent of sodium diacetate; the solvent mainly comprises 5 to 20 weight percent of ethanol, 3 to 5 weight percent of isopropyl alcohol and 75 to 90 weight percent of water; the weight ratio of the solute to the solvent is 3-7:93-97; and the bacillus is Bacillus subtilis, Bacillus licheniformis and Bacillus megatherium, and the total bacteria count of the bacillus is 2*10<4>CFU/ml-1*10<6>/ml magnitude order. The detergent has obvious deodorization effect, is clean, non-irritating, nontoxic and harmless, is quick and complete in biodegradation, reduces the content of organic substances in waste cleaning water, further reduces the aftertreatment cost of the waste water, has good economic benefit, environment-friendly benefit and social benefit and superior performance, and cannot cause secondary pollution.
Description
Technical field
The present invention relates to environment protection and daily cleaning product scope, relate in particular to a kind of novel environmental protection and contain sanitising agent of microorganism and preparation method thereof.
Background technology
Along with the develop rapidly of society, stench, eutrophication pollution have brought huge harm for our life.
Odorant pollutant is meant that all stimulate that olfactory organs cause that people detest, unhappy and infringement HUMAN HEALTH and influence the gas pollutant of ambient air quality.It is unhappy that stink is given sense organ, even entail dangers to Human Physiology health, such as expiratory dyspnea, fall stomach, uncomfortable in chest, vomiting etc.The amendment of U.S.'s " clean air bill " nineteen ninety has been worked out more severe atmospheric emission standard, 8 kinds of original obnoxious flavour pollutents are increased to 188 kinds, mainly be based on carinogenicity, mutagenicity and the genotoxicity of these chemical substances, and enumerated source of pollution with these gases of potential emission.China formulated the odor pollution emission standard in 1993, and nineteen ninety-five, revised " The Law of the People's Republic of China on the Prevention and Control of Atmospheric Pollution " also stipulated: " to the pollutant discharging unit of airborne release foul gas, must take measures to prevent that the surrounding resident district from being polluted ".Along with the continuous development of China's industrial economy, odor pollution has become the air pollution problems inherent that people generally are concerned about after dust, sulfurous gas, oxynitride.
The foul gas complicated component, wide material sources, the industry that relates to is a lot, all can produce a great deal of, different types of stench and toxic gas as manufacturing enterprises such as urban domestic garbage transfer station, refuse landfill, sewage work, sludge treatment factory and petrochemical complex, paints, especially in densely populated big and medium-sized cities, relevant in recent years odor pollution tip-offs about environmental issues constantly increase, and stench has become the important problem of environmental pollution that needs to be resolved hurrily.
Maintaining people's existence, ensureing economic construction and safeguarding that the importance of water needless to say in all physical features of social development.Yet along with industrialization, rapid urbanization, the world is faced with shortage of water resources, with serious pollution challenge.
China is especially serious, be one of 13 lack of water countries in the world, present only about half of urban water shortage in more than 600 city, the whole nation, the deterioration that water pollutes more makes water shortage make the matter worse: China's rivers and lakes are generally polluted, and eutrophication has in various degree appearred in the lake in the whole nation 75%; 90% city coin water pollution is serious, and the 60%---70% of the total water deficit in southern city causes owing to water pollutes; Subterranean water enquire to 118 big and medium-sized cities of China shows that have 115 urban groundwaters to be polluted, wherein serious pollution accounts for 40%.Water pollutes the functions of use that has reduced water body, has aggravated shortage of water resources, and China's implementation of sustainable development has been brought negative impact.
China's water pollution is mainly from two aspects, and the one, the industrial development discharging trade effluent that exceeds standard, the 2nd, because urban sewage discharge and facility for the central disposal famine, a large amount of unprocessed water bodys that directly enter of sanitary sewage cause environmental pollution in the urbanization.Though trade effluent reduces through administering to some extent in recent years, city domestic sewage is growing on and on, and accounts for more than 51% of water pollution.According to environment department's monitoring, the unprocessed rivers,lakes and seas that directly enter of sanitary sewage in the whole nation nearly 80% in 1999, a year blowdown flow rate reaches 400 billion cubic meters, causes 1/3rd above waters, the whole nation to be polluted.
China's water pollution accident in recent years takes place frequently, and particularly since last year, Taihu Lake, Chaohu, Dian Chi are broken out blue-green algae in succession on a large scale, and some important drinking water sources are polluted, and have a strong impact on the masses' productive life and social stability.
The existing sanitising agent of existing market contains benzene, zeolite, enzyme, white dyes mostly mainly based on the petrochemical complex sanitising agent.But along with the exhaustion day by day of petroleum resources, the prospect of chemosynthesis clean-out system allows of no optimist.Though the use of chemically cleaning lotion is greatly improved people's quality of life, our survival environment is polluted, human health also is subjected to serious harm simultaneously.Therefore people must produce vigilance to the chemically cleaning lotion, seek human and natural toxicological harmless and are enough to the cleaner in environmental protection of substituted chemistry lotion.
Chinese patent (patent No. CN1919991A) has been introduced a kind of liquid washing agent that does not have sequestrant and inorganic assistant agent, does not consider the friendly degree of its washed product to environment, and its washing consumption be difficult to control, foam volume is big, is unfavorable for the conserve water resource.Chinese patent (patent No. CN1322802) is pointed out, for improving washing effect, adds an amount of sterilization wood powder in existing liquid washing agent, effectively improves the soil removability of liquid washing agent.But life-time service can bring certain stimulation injury to skin.
Commercially available common washings just has certain cleaning performance to slight spot mostly, for such as when having dinner accidentally or be stained with in the work and various severe grease mottlings often show powerless.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of sanitising agent that contains microorganism and preparation method thereof is provided.
The objective of the invention is to be achieved through the following technical solutions:
The invention has the beneficial effects as follows: sanitising agent cleaning deodorizing effect of the present invention is remarkable, non-stimulated, nontoxic; Can not only remove organism, and can in washes or cleaning medium, realize that quick degraded falls in biology; High comprehensive performance, do not cause secondary pollution; Can be used as in the washing liquid, washing composition, carpet cleaner, car sanitising agent, water drain sanitising agent, cupboard defends product form such as a deodorizing composition and occurs.
Embodiment
Microorganism cleaning agent of the present invention mainly is made up of solute, solvent and genus bacillus.The weight proportion of solute and solvent is 3～7:93～97; Genus bacillus is subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformus) and bacillus megaterium (Bacillus megaterium), and its total count is 2 * 10
4CFU/ml-1 * 10
6Individual/the ml order of magnitude.
Wherein, solute is that the alkylphenol polyoxyethylene-10 of 55%-70%, the stearin of 29%-44.5% and two sodium-acetates of 0.5%-1% are formed by weight percent mainly.Solvent is that the ethanol of 5%-20%, the Virahol of 3%-5% and the water of 75%-90% are formed by weight percent mainly.
The present invention contains the preparation method of the sanitising agent of microorganism, may further comprise the steps:
1, preparation solvent: according to weight proportion, get the ethanol of 5%-20%, the Virahol of 3%-5% and the water of 75%-90%, mix.
2, preparation solute: according to weight proportion, get the alkylphenol polyoxyethylene-10 of 55%-70%, the stearin of 29%-44.5% and two sodium-acetates of 0.5%-1%, mix.
3, cultivate seed liquor: will place 27-32 ℃ constant temperature shaking table shaking culture in subtilis (Bacillus subtilis), Bacillus licheniformis (Bacilluslicheniformus), three kinds of bacterial classification liquid medium withins of bacillus megaterium (Bacillus megaterium) respectively, reach 1 * 10 to the bacterium number
7CFU/ml-1 * 10
8Individual/as more than the ml order of magnitude, to regulate pH to 6.0-7.0 then, make seed liquor through sterilising treatment.
The composition of substratum and the weight proportion of each component are: extractum carnis: peptone: sodium-chlor: glucose: dipotassium hydrogen phosphate=1-3:1-3:1-3:1-3:1-3 (pH transfers to 6.5-7.5).
The potential of hydrogen of substratum should meet the bacterial growth requirement.Accurately measure adjusting pH value by various substratum requirements, can utilize to drip NaOH or HCl, H
2SO
4Carry out pH regulator.Sterilization can be handled by high-pressure sterilizing pot.
4,2～10:1000 gets seed liquor and solvent by volume, mixes, and gets mixed solution.
5, according to weight proportion 3～7:93～97 of solute and solvent, get solute, be added to mixed solution, mix, promptly make the sanitising agent that the present invention contains microorganism.
The sanitising agent that the present invention contains microorganism at normal temperatures, validity period can reach more than 2 years.
The microorganism washing composition includes specified microorganisms and bio-surfactant.Tensio-active agent changes interfacial surface tension by a series of effects such as solubilisings, promotes organic dissolving and decomposition.Bio-surfactant is the tensio-active agent that Institute of Micro-biology produces, and is similar with chemical surfactant, do not participate in any chemical reaction, but by changing boundary's surface tension, helps the absorption of microorganism to pollutent.
Bacillus subtillis, Bacillus licheniformis, bacillus megaterium etc. all have the ability of foul gass such as fine degraded grease and ammonia nitrogen, and these three kinds of bacterial classifications can be secreted bio-surfactant, and human body is not had harm, and are environmentally friendly.
Genus bacillus can form spore in microorganism washing composition of the present invention, can form spore, and the spore wall thickness can be resisted poor environment.Under conditions such as drying, low temperature and long-term dark, can form the akinete of dormant state, but when continued growth under optimum conditions.。Adding the genus bacillus that contains among the present invention toward the water body that contains organic waste after one rational period, bacillus recovers active and continued growth (from the dormant state to the active condition), produces certain enzyme and degraded and digestion organic waste.The microorganism that adds is in the logarithmic phase of cell---can double every 20 to 30 minutes bacteriums.The by product of this bacterial strain/enzyme is water and carbonic acid gas, environmental sound.When gemma was placed into municipal effluent sewage work, this situation will take place.At last, the microorganism of inoculation will form and contain various genus bacillus polymers, will have lasting soil removability simultaneously.Thereby solve various water pollution problemss at all.
This microorganism cleaning agent finally can reach and reduce COD, BOD and by eliminating organism, reduce the effect of foul gas quantity discharged, is difficult for causing secondary pollution, is a big focus of following sanitising agent industry.
Embodiment 1
In the 500ml volumetric flask, add 5% (g/g) ethanol, 5% (g/g) Virahol, 90% (g/g) water.Be the constant temperature shaking table shaking culture of 150rpm with placing 27 ℃, rotating speed in subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformus), three kinds of bacterial classification liquid medium withins of bacillus megaterium (Bacillus megaterium) respectively, reach 1 * 10 at least to the bacterium number
8Individual/as can to take down seed liquor from shaking table more than the ml order of magnitude.With sulfuric acid with pH regulator to 7.0.In fermentor tank,, make seed liquor, and add in the solvent at 105 ℃ of 15min that sterilize down.Add solute at mixed solution at last, the solute composition is 55% (g/g) alkylphenol polyoxyethylene-10,44% (g/g) stearin and the two sodium-acetates of 1% (g/g), promptly makes the agent of this microorganism cleaning.
Embodiment 2
In the 500ml volumetric flask, add 20% (g/g) ethanol, 3% (g/g) Virahol, 77% water (g/g).Be the constant temperature shaking table shaking culture of 150rpm with placing 30 ℃, rotating speed in subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformus), three kinds of bacterial classification liquid medium withins of bacillus megaterium (Bacillus megaterium) respectively, reach 1 * 10 at least to the bacterium number
7Can take down seed liquor from shaking table more than the CFU/ml-order of magnitude.With sulfuric acid with pH regulator to 6.0.In fermentor tank,, make seed liquor, and add in the solvent at 105 ℃ of 15min that sterilize down.Add solute at mixed solution at last, the solute composition is 59.5% (g/g) alkylphenol polyoxyethylene-10,40% (g/g) stearin and the two sodium-acetates of 0.5% (g/g), promptly makes the agent of this microorganism cleaning.
Embodiment 3
In the 500ml volumetric flask, add 10% (g/g) ethanol, 4% (g/g) Virahol, 86% water (g/g).Be the constant temperature shaking table shaking culture of 150rpm with placing 32 ℃, rotating speed in subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformus), three kinds of bacterial classification liquid medium withins of bacillus megaterium (Bacillus megaterium) respectively, reach 1 * 10 at least to the bacterium number
8Individual/as can to take down seed liquor from shaking table more than the ml order of magnitude.With sulfuric acid with pH regulator to 7.0.In fermentor tank,, make seed liquor, and add in the solvent at 105 ℃ of 15min that sterilize down.Add solute at mixed solution at last, the solute composition is 70% (g/g) alkylphenol polyoxyethylene-10,29.5% (g/g) stearin and 0.5%g/g) two sodium-acetates, promptly make the agent of this microorganism cleaning.
Embodiment 4
Under the room temperature, in the basin that the 2L tap water is housed, add 8ml microorganism cleaning agent A, traditional washing liquid B, traditional washing liquid C, tap water D respectively.After leaving standstill 1.5h, observe the opacity of laundry effect and waste water.From laundry effect, A=B=C〉D, i.e. the laundry effect of three kinds of sanitising agents difference is not obvious, and decontamination all preferably is to reach the purpose of cleaning.From the opacity of waste water, B〉D〉A〉C, C and A are comparatively limpid, and B is creamy white, and D is a tap water, does not add any sanitising agent, and its oily matter is more, and the pasta and the water surface are apparent in view.
Embodiment 5
Under the room temperature,, clean with microorganism cleaning agent, traditional chemical carpet cleaner respectively equally by the polluted carpet of spot.Same dosage, the traditional chemical carpet cleaner can only clean the top layer, and adopts the fragrance that self distributes to cover the peculiar smell of carpet, inside that can't cleaning carpet, carpet inside is only the place of bacterium and Parasites breeding in fact.And the microorganism cleaning agent can be goed deep into carpet inside, more thoroughly cleans carpet under the effect of bacterium, and free from extraneous odour leaves over, and the performance gentleness is harmless to the surface, does not have corrosion.
The foregoing description is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.
Claims (5)
1, a kind of sanitising agent that contains microorganism is characterized in that, it mainly is made up of solute, solvent and genus bacillus.Wherein, the weight proportion of solute and solvent is 3～7:93～97.Genus bacillus is subtilis, Bacillus licheniformis and bacillus megaterium, and its total count is 2 * 10
4CFU/ml-1 * 10
6Individual/the ml order of magnitude.
2, according to the described sanitising agent that contains microorganism of claim 1, it is characterized in that described solute is that the alkylphenol polyoxyethylene-10 of 55%-70%, the stearin of 29%-44.5% and two sodium-acetates of 0.5%-1% are formed by weight percent mainly.
According to the described sanitising agent that contains microorganism of claim 1, it is characterized in that 3, described solvent is that the ethanol of 5%-20%, the Virahol of 3%-5% and the water of 75%-90% are formed by weight percent mainly.
4, a kind of preparation method who contains the sanitising agent of microorganism is characterized in that, may further comprise the steps:
(1) preparation solvent: according to weight proportion, get the ethanol of 5%-20%, the Virahol of 3%-5% and the water of 75%-90%, mix.
(2) preparation solute: according to weight proportion, get the alkylphenol polyoxyethylene-10 of 55%-70%, the stearin of 29%-44.5% and two sodium-acetates of 0.5%-1%, mix.
(3) cultivate seed liquor: will place 27-32 ℃ constant temperature shaking table shaking culture in subtilis (Bacillus subtilis), Bacillus licheniformis (Bacilluslicheniformus), three kinds of bacterial classification liquid medium withins of bacillus megaterium (Bacillus megaterium) respectively, reach 1 * 10 to the bacterium number
7CFU/ml-1 * 10
8Individual/as more than the ml order of magnitude, to regulate pH to 6.0-7.0 then, make seed liquor through sterilising treatment.
(4) 2～10:1000 gets seed liquor and solvent by volume, mixes, and gets mixed solution.
(5) according to weight proportion 3～7:93～97 of solute and solvent, get solute, be added to mixed solution, mix, make the sanitising agent that the present invention contains microorganism.
5, according to the described preparation method of claim 4, it is characterized in that, in the described step (3), the composition of described substratum and the weight proportion of each component are: extractum carnis: peptone: sodium-chlor: glucose: dipotassium hydrogen phosphate=1-3:1-3:1-3:1-3:1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100956758A CN101475882B (en) | 2009-01-19 | 2009-01-19 | Cleanser containing microorganism and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100956758A CN101475882B (en) | 2009-01-19 | 2009-01-19 | Cleanser containing microorganism and preparation thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101475882A true CN101475882A (en) | 2009-07-08 |
| CN101475882B CN101475882B (en) | 2011-07-20 |
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ID=40836661
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100956758A Expired - Fee Related CN101475882B (en) | 2009-01-19 | 2009-01-19 | Cleanser containing microorganism and preparation thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102785636A (en) * | 2012-05-19 | 2012-11-21 | 贺卫初 | Environment-friendly, rinse-free and zero-emission train (subway or locomotive) cleaning technology |
| CN105695158A (en) * | 2016-03-15 | 2016-06-22 | 王玉兴 | Microorganism antibacterial toilet cleaner |
| US9745597B2 (en) | 2014-05-28 | 2017-08-29 | Bayer Cropscience Lp | Compositions and methods for controlling fungal and bacterial diseases in plants |
| CN110980960A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Oil-removing microbial inoculum and sludge treatment technology using same |
| WO2021240315A1 (en) * | 2020-05-27 | 2021-12-02 | Setter Dylan John | Probiotic bacillus sanitiser |
| CN113913251A (en) * | 2021-09-16 | 2022-01-11 | 安徽克菱保健科技有限公司 | Microbial washing sheet for washing tableware |
-
2009
- 2009-01-19 CN CN2009100956758A patent/CN101475882B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102785636A (en) * | 2012-05-19 | 2012-11-21 | 贺卫初 | Environment-friendly, rinse-free and zero-emission train (subway or locomotive) cleaning technology |
| US9745597B2 (en) | 2014-05-28 | 2017-08-29 | Bayer Cropscience Lp | Compositions and methods for controlling fungal and bacterial diseases in plants |
| CN105695158A (en) * | 2016-03-15 | 2016-06-22 | 王玉兴 | Microorganism antibacterial toilet cleaner |
| CN110980960A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Oil-removing microbial inoculum and sludge treatment technology using same |
| WO2021240315A1 (en) * | 2020-05-27 | 2021-12-02 | Setter Dylan John | Probiotic bacillus sanitiser |
| CN113913251A (en) * | 2021-09-16 | 2022-01-11 | 安徽克菱保健科技有限公司 | Microbial washing sheet for washing tableware |
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| Publication number | Publication date |
|---|---|
| CN101475882B (en) | 2011-07-20 |
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