CN101927014A - Biological peculiar smell scavenger and production method thereof - Google Patents
Biological peculiar smell scavenger and production method thereof Download PDFInfo
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- CN101927014A CN101927014A CN2010102540665A CN201010254066A CN101927014A CN 101927014 A CN101927014 A CN 101927014A CN 2010102540665 A CN2010102540665 A CN 2010102540665A CN 201010254066 A CN201010254066 A CN 201010254066A CN 101927014 A CN101927014 A CN 101927014A
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Abstract
The invention discloses a biological peculiar smell scavenger and a production method thereof. The scavenger comprises bacillus megaterium (CGMCC 1.2393), candida utillis (CGMCC 2.1012) and azotobacter chroococcum (CGMCC 1.2391). The production method for the scavenger comprises the following steps of: inoculating the activated bacillus megaterium, candida utillis and azotobacter chroococcum into a silicate group bacteria culture medium, a wort culture medium and a nitrogen-fixing culture medium to perform seed liquid culture respectively; and mixing the three seed liquids in a mass part ratio of 0.5-1.5: 1-2: 1-2, and adjusting the concentration of the mixed solution as required. The biological peculiar smell scavenger sprayed to a place with peculiar smell can remarkably reduce the stench TVOC, particularly has obvious effect of reducing ammonia, aldehyde and benzene, and has the advantages of low cost, no secondary pollution and convenient use.
Description
Technical field
The present invention relates to the biological environmental production field, relate in particular to a kind of biological peculiar smell scavenger and production method thereof of utilizing microorganism species to remove abnormal flavour.
Background technology
Newly purchase the foul smell that usually gives off a peculiar smell after automobile and the house fitting-up, these abnormal flavour foul smell are mainly some escaping gas TVOC, as Ammonia, benzene class, aldehydes etc.Its gas of these volatility is very harmful to health, and some also have carcinogenesis, becomes the recessive killer in the life.The method of traditional these gases of reduction generally has two classes, i.e. physisorphtion and chemical method.But traditional physics and chemical method are not very desirable, have problems such as cost height, secondary pollution be serious.
Summary of the invention
The ultimate principle of microbial treatments abnormal flavour be utilize microorganism the abnormal flavour molecule absorption in microorganism in health, make a kind of process of its degraded by the metabolic activity of microorganism.Basically be divided into three processes: the 1. course of dissolution of abnormal flavour molecule, promptly changed into the mass transport process of liquid phase by gas phase; 2. the abnormal flavour molecule is absorbed by microorganism by cells of microorganisms wall and cell membrane, and water-fast abnormal flavour molecule is earlier attached to outside the microbial body, is decomposed into solable matter by the exoenzyme of microorganism secretion, infiltrates cell again; 3. after the abnormal flavour molecule enters cell, be that Institute of Micro-biology decomposes, utilizes, makes abnormal flavour to be removed as nutrient substance in vivo.
At the problems referred to above, provide a kind of biological peculiar smell scavenger that utilizes microorganism species to remove abnormal flavour.
Another object of the present invention provides a kind of production method of producing odor scavenging agent.
Technical scheme of the present invention is: a kind of biological peculiar smell scavenger comprises bacillus megaterium (Bacillus megaterium CGMCC 1.2393), produces Ruan's candida mycoderma (Candida utilis CGMCC 2.1012), brown ball nitrogen fixing bacteria (Azotobacter chroococcum CGMCC 1.2391).
As preferably, described bacillus subtilis, the mass ratio that produces Ruan's candida mycoderma, brown ball nitrogen fixing bacteria are 0.5~1.5: 1~2: 1~2.
As preferably, described bacillus subtilis, the mass ratio that produces Ruan's candida mycoderma, brown ball nitrogen fixing bacteria are 0.75: 1.5: 1.5.
A kind of production method of biological peculiar smell scavenger may further comprise the steps:
1) will be through the bacillus megaterium of activation processing, produce Ruan's candida mycoderma and brown ball nitrogen fixing bacteria and inoculate respectively and carry out seed liquor in silicate bacteria culture medium, malt extract medium and the fixed nitrogen culture medium and cultivate;
2) 3 kinds of seed liquor in the step 1) are pressed mass fraction 0.5~1.5: mix at 1~2: 1~2, adjusts mixed liquid concentration as required.
Bacillus megaterium (Bacillus megaterium CGMCC 1.2393), to produce Ruan's candida mycoderma (Candida utilis CGMCC 2.1012), brown ball nitrogen fixing bacteria (Azotobacter chroococcum CGMCC 1.2391) all open available from Chinese common micro-organisms bacterial strain preservation administrative center, and three kinds of bacterium are safe non-pathogenic bacteria.
The invention has the beneficial effects as follows:
1. after this biological peculiar smell scavenger is sprayed in the place with abnormal flavour, can significantly reduce foul smell TVOC, particularly obvious to reducing Ammonia, aldehydes and benzene class effect.
2. low, the non-secondary pollution, easy to use of cost.
The specific embodiment
The invention will be further described below by specific embodiment.
Embodiment 1: a kind of biological peculiar smell scavenger comprises bacillus megaterium (Bacillus megaterium CGMCC 1.2393), produces Ruan's candida mycoderma (Candida utilis CGMCC 2.1012), brown ball nitrogen fixing bacteria (Azotobacter chroococcum CGMCC 1.2391).
Embodiment 2: a kind of biological peculiar smell scavenger comprises bacillus megaterium (Bacillus megaterium CGMCC 1.2393), produces Ruan's candida mycoderma (Candida utilis CGMCC 2.1012), brown ball nitrogen fixing bacteria (Azotobacter chroococcum CGMCC 1.2391).The mass ratio of bacillus subtilis, product Ruan candida mycoderma, brown ball nitrogen fixing bacteria is 0.5: 1: 1.
Embodiment 3: a kind of biological peculiar smell scavenger comprises bacillus megaterium (Bacillus megaterium CGMCC 1.2393), produces Ruan's candida mycoderma (Candida utilis CGMCC 2.1012), brown ball nitrogen fixing bacteria (Azotobacter chroococcum CGMCC 1.2391).The mass ratio of bacillus subtilis, product Ruan candida mycoderma, brown ball nitrogen fixing bacteria is 1.5: 2: 2.
Embodiment 4: a kind of biological peculiar smell scavenger comprises bacillus megaterium (Bacillus megaterium CGMCC 1.2393), produces Ruan's candida mycoderma (Candida utilis CGMCC 2.1012), brown ball nitrogen fixing bacteria (Azotobacter chroococcum CGMCC 1.2391).The mass ratio of bacillus subtilis, product Ruan candida mycoderma, brown ball nitrogen fixing bacteria is 0.75: 1.5: 1.5.
Embodiment 5: a kind of production method of biological peculiar smell scavenger may further comprise the steps:
1) will be through the bacillus megaterium of activation processing, produce Ruan's candida mycoderma and brown ball nitrogen fixing bacteria and inoculate respectively and carry out seed liquor in silicate bacteria culture medium, malt extract medium and the fixed nitrogen culture medium and cultivate.
The silicate bacteria culture medium prescription is: sucrose 5.0g, sodium hydrogen phosphate 2.0g, MgSO47H
2O 0.5g, CaCO
30.1g, soil mineral 1.0g, distilled water 1L, pH7.0-7.2.
The malt extract medium prescription is: beerwort 150ml, distilled water 1L, pH nature.
The fixed nitrogen culture medium prescription is: potassium dihydrogen phosphate 0.2g, dipotassium hydrogen phosphate 0.8g, MgSO
47H
2O 0.2g, CaSO
4.2H
2O 0.1g, Na
2MoO
42H
2The O trace, yeast extract 0.5g, mannitol 20g, agar 15g, distilled water 1.0L, pH7.2.
Every sampling in 2 hours, when cell concentration reaches 1 * 10
6During/ml, stop seed liquor and cultivate.
2) 3 kinds of seed liquor in the step 1) are pressed mass fraction 0.5~1.5: mix at 1~2: 1~2, adjusts mixed liquid concentration as required, adds an amount of essence.
The deodorizing effect experiment
Experimental example 1: get family kitchen and wash rice, wash dish, wash combined sewage such as fish, with its place gave out a foul smell in several days after, get 1L sewage and be sprayed into biological peculiar smell scavenger 10ml, obviously reduce by the subjective appreciation stench after 4 hours.
Experimental example 2: get biological peculiar smell scavenger 100ml and be sprayed on content of formaldehyde at the 0.5mg/ cubic meter, area is in the air of 20 square metres of new decorating houses, and the Detection of Air Quality result is the 0.07mg/ cubic meter for content of formaldehyde after three days.
Experimental example 3: getting biological peculiar smell scavenger 1000ml, to be sprayed on ammonia level be the 2.5mg/ cubic meter, and area is that ammonia level is reduced to the 0.8mg/ cubic meter after one day in 10 square metres the pigsty.
Experimental example 4: getting biological peculiar smell scavenger 200ml, to be sprayed on benzene content be newly purchasing in the automobile of 0.38mg/ cubic meter, and benzene content is reduced to the 0.19mg/ cubic meter after one day, and benzene content is reduced to the 0.08mg/ cubic meter after three days.
Claims (4)
1. a biological peculiar smell scavenger is characterized in that: comprise bacillus megaterium (Bacillus megaterium CGMCC 1.2393), produce Ruan's candida mycoderma (Candida utilis CGMCC 2.1012), brown ball nitrogen fixing bacteria (Azotobacter chroococcum CGMCC 1.2391).
2. biological peculiar smell scavenger according to claim 1 is characterized in that: the mass ratio of described bacillus subtilis, product Ruan candida mycoderma, brown ball nitrogen fixing bacteria is 0.5~1.5: 1~2: 1~2.
3. biological peculiar smell scavenger according to claim 1 is characterized in that: the mass ratio of described bacillus subtilis, product Ruan candida mycoderma, brown ball nitrogen fixing bacteria is 0.75: 1.5: 1.5.
4. the production method of biological peculiar smell scavenger according to claim 1 is characterized in that: may further comprise the steps:
1) will be through the bacillus megaterium of activation processing, produce Ruan's candida mycoderma and brown ball nitrogen fixing bacteria and inoculate respectively and carry out seed liquor in silicate bacteria culture medium, malt extract medium and the fixed nitrogen culture medium and cultivate;
2) 3 kinds of seed liquor in the step 1) are pressed mass fraction 0.5~1.5: mix at 1~2: 1~2, adjusts mixed liquid concentration as required.
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CN201010254066.5A CN101927014B (en) | 2010-08-16 | 2010-08-16 | Biological peculiar smell scavenger and production method thereof |
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CN101927014A true CN101927014A (en) | 2010-12-29 |
CN101927014B CN101927014B (en) | 2014-04-02 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105032169A (en) * | 2015-07-22 | 2015-11-11 | 江西美丽家居生态环保有限公司 | Production method for microorganism metabolite organic formaldehyde removing agent |
CN109833760A (en) * | 2019-02-27 | 2019-06-04 | 北京森家环保科技发展有限公司 | A kind of composition and its preparation method of indoor deodoring |
CN109876644A (en) * | 2019-02-18 | 2019-06-14 | 王玉廷 | A kind of biological deodorant and the preparation method and application thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1861199A (en) * | 2005-05-12 | 2006-11-15 | 中国农业大学 | Biological deodorization and purification agent, and its use |
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2010
- 2010-08-16 CN CN201010254066.5A patent/CN101927014B/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1861199A (en) * | 2005-05-12 | 2006-11-15 | 中国农业大学 | Biological deodorization and purification agent, and its use |
Non-Patent Citations (2)
Title |
---|
叶芬霞等: "复合微生物吸附除臭剂的制备及其除臭应用", 《农业工程学报》, vol. 24, no. 8, 31 August 2008 (2008-08-31), pages 254 - 257 * |
李淑芹等: "微生物脱臭的技术研究", 《东北农业大学学报》, vol. 34, no. 1, 31 March 2003 (2003-03-31), pages 100 - 104 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105032169A (en) * | 2015-07-22 | 2015-11-11 | 江西美丽家居生态环保有限公司 | Production method for microorganism metabolite organic formaldehyde removing agent |
CN109876644A (en) * | 2019-02-18 | 2019-06-14 | 王玉廷 | A kind of biological deodorant and the preparation method and application thereof |
CN109833760A (en) * | 2019-02-27 | 2019-06-04 | 北京森家环保科技发展有限公司 | A kind of composition and its preparation method of indoor deodoring |
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CN101927014B (en) | 2014-04-02 |
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