CN1014427B - Muscardine culturing method with solid medium - Google Patents
Muscardine culturing method with solid mediumInfo
- Publication number
- CN1014427B CN1014427B CN 86100658 CN86100658A CN1014427B CN 1014427 B CN1014427 B CN 1014427B CN 86100658 CN86100658 CN 86100658 CN 86100658 A CN86100658 A CN 86100658A CN 1014427 B CN1014427 B CN 1014427B
- Authority
- CN
- China
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- raw material
- soil
- muscardine
- calcium magnesium
- wheat bran
- Prior art date
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- Expired
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a solid culture method of microbial insecticide-white muscardine fungi. Besides partial wheat bran or fine rice bran or flour exists in culture raw material, the method has the characteristics that soil is used as the main raw material in the culture raw material (a small quantity of carbamide and fused calcium magnesium phosphate is added), wherein the proportion of the soil is from 50 to 85%. The white muscardine fungi powder produced by the method has the advantages of good prevention and curing effects, good dispersity, far diffusion distance, simple production process, local material adoption, sufficient raw material source, low cost, high economic benefit and easy popularization.
Description
The invention belongs to a kind of method of producing by indigenous method of microbial pesticide.
Muscardine (Beauveria) is a kind of imperfect fungi parasitical fungi, belongs to the Neurospora order, Neurospora section, Beauveria.It is a kind of entomophagous parasite bacterium of broad spectrum, can parasitize various insects and mite class.Its spore drops on the polypide, can germinate under certain condition and directly invade in the insect body, thereby a large amount of propagation also produce spore and make the insect ossified shape that is white in color, in forestry and agriculture production, people utilize muscardine to come pest control as pine moth, Pyrausta nubilalis (Hubern)., eating-core bean worm and the snout moth's larva of rice etc.Be a kind of effective microbial pesticide, it is easy to use, and is harmless to the nontoxic natural enemy to insect of people and animals, and superinfection and the insecticidal action that spreads of diffusion are arranged, can not only pest control, free from environmental pollution, also help the protection eubiosis.
At present, the cultivation raw material of solid culture muscardine production usefulness generally is to add chaff, husk or sawdust about 30% with about 70% wheat bran or fine rice bran, and the latter mainly makes stopping composition, plays the effect of cooling, hydrofuge, ventilation during the fermentation.But it is to store longly slightly that these materials are made the shortcoming of stopping composition, and sterilization has carelessness to pollute and cause the fermentation failure with regard to easily causing slightly.Make the generation material with bagasse, bright pine needle and also can obtain ferment successfully, but this method is time-consuming, and gather bright pine needle in a large number and cause excessive pruning to get leaf can to influence tree growth, thus also inadvisable.
The method that the purpose of this invention is to provide a kind of improved solid culture muscardine, it can guarantee that cultivating raw material with original formulation has the identical high advantage of fermentation rate height, spore content, can reduce the consumption of grain by-products such as wheat bran, fine rice bran again significantly, thereby reduce production costs greatly, can gather materials on the spot simultaneously, the source is sufficient.
Invention is to realize like this, with wheat bran or fine rice bran or flour mixing do cultivation raw material, add certain moisture and adequate nutrition growth hormone, wherein soil accounts for not necessarily loam of the used soil of the 50-85%(that cultivates the raw material total amount, upper, middle and lower layer soil all can be used), the element of nourishing and growing is 1% urea and 2% fused(calcium magnesium)phosphate.After above-mentioned mixed uniform cultivation raw material sterilising treatment, necessarily to inoculate with one-level muscardine solid seed under acid, the basicity, cultivation got final product in 4-7 days under the normal temperature.
The present invention can gather materials on the spot because be main cultivation raw material with soil, sufficient raw and economy has reduced traffic capacity simultaneously, thus reduce cost greatly.Because soil is made stopping composition such as raw material ratio husk, bagasse, chaff and pulverized easily, can fully disperse simultaneously, therefore diffusion easily, diffusion length is far away, can reach 200 meters farthest, helps spreading of muscardine, thereby good disinsection effect.
The concrete grammar of invention is by general flow chart shown in the accompanying drawing 1 and embodiment explanation
The solid of production one-level according to a conventional method muscardine seed.
The secondary solid enlarges to be produced: soil with 70% and 30% wheat bran mix, with stirring in the compound that is added to soil and wheat bran after the fused(calcium magnesium)phosphate dissolving of the water of the 25-30% of raw material gross weight with 1% urea and 2%, the cultivation raw material of mixing is sub-packed in the osculum culturing bottle, is suitable with 1/3rd places that install to container.Bottleneck is built with kraft paper and is tightened, and at high temperature carries out sterilising treatment.General Temperature Treatment 2 hours more than 90 ℃ is taken out material then and is cooled to aseptic technique at once about 30 ℃, inserts one-level solid muscardine seed, and inoculation is not all the 1-3% of raw material gross weight according to temperature.A general test tube solid seed (about about 3 grams) can be inoculated one bottle of secondary solid culture raw material (about 300-400 gram).Cultivate under normal temperature laboratory the inoculation back can form muscardine in 4-7 days.Bottle outlet after drying, pulverizing promptly obtain product after sieving with 80 mesh sieve.
Produce muscardine in this way and both can reduce the operation that three grades big beds are cultivated, also can obtain the muscardine powder yield of some amount, then can carry out three grades big bed cultivations on this basis if need to increase considerably output in the reality, its inoculum size is for cultivating the 35-40% of raw material gross weight, and normal temperature is cultivated down and got final product in 4-7 days.
In addition, use following prescription also can obtain effect same, its production method is identical with embodiment 1 with step.
2, native 70%+ fine rice bran 30%+ urea 1%+ fused(calcium magnesium)phosphate 2%
3, native 75%+ wheat bran 25%+ urea 1%+ fused(calcium magnesium)phosphate 2%
4, native 85%+ flour 15%+ urea 1%+ fused(calcium magnesium)phosphate 2%
Claims (1)
1, a kind of microbial pesticide--the plate method of muscardine (Beauveria) is characterized in that:
A, usefulness soil and wheat bran or flour mixed culture raw material after the water and the plain sterilising treatment of nourishing and growing, are inoculated up the production method of fermenting with one-level muscardine solid seed,
B, above-mentioned with soil during with wheat bran or fine rice bran or flour mixed culture raw material the shared ratio of soil be 50-85%, and used soil loam not necessarily, general soil all can be used,
C, add urea and fused(calcium magnesium)phosphate as nourishing and growing element, wherein urea account for cultivate the raw material gross weight 1%, fused(calcium magnesium)phosphate accounts for and cultivates 2% of raw material gross weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86100658 CN1014427B (en) | 1986-01-21 | 1986-01-21 | Muscardine culturing method with solid medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86100658 CN1014427B (en) | 1986-01-21 | 1986-01-21 | Muscardine culturing method with solid medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN86100658A CN86100658A (en) | 1987-10-07 |
| CN1014427B true CN1014427B (en) | 1991-10-23 |
Family
ID=4801143
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 86100658 Expired CN1014427B (en) | 1986-01-21 | 1986-01-21 | Muscardine culturing method with solid medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1014427B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067718C (en) * | 1995-07-31 | 2001-06-27 | 中国农业工程研究设计院 | Microorgan and its opsonin |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065111C (en) * | 1997-10-21 | 2001-05-02 | 哈尔滨市荣华生物技术开发公司 | Preparation of bacteriological insecticide with fly-ash as carrier |
| CN104804071B (en) * | 2014-01-29 | 2018-01-09 | 中国科学院海洋研究所 | A kind of depside peptides and its preparation method and application |
| CN103918725B (en) * | 2014-04-18 | 2016-06-29 | 安徽省淮北市农药厂 | Utilize the method that wheat bran prepares antibacterial |
-
1986
- 1986-01-21 CN CN 86100658 patent/CN1014427B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067718C (en) * | 1995-07-31 | 2001-06-27 | 中国农业工程研究设计院 | Microorgan and its opsonin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN86100658A (en) | 1987-10-07 |
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