CN1065111C - Preparation of bacteriological insecticide with fly-ash as carrier - Google Patents
Preparation of bacteriological insecticide with fly-ash as carrier Download PDFInfo
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- CN1065111C CN1065111C CN97118839A CN97118839A CN1065111C CN 1065111 C CN1065111 C CN 1065111C CN 97118839 A CN97118839 A CN 97118839A CN 97118839 A CN97118839 A CN 97118839A CN 1065111 C CN1065111 C CN 1065111C
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Abstract
The present invention provides technology for preparing biologic bacterium insecticide by using the powder dust of pulverized coal ash as carriers, and relates to the processing technology of biologic pesticide insecticide. Pure spore powder is used as original seed which is formed into liquid seed containing a large amount of bacterium rubber balls through two-stage liquid fermentation, the liquid seed is mixed with 10 portions of powder dust of pulverized coal ash over 300 meshes after being mixed with nutritive liquid in the proportion of 1: 3, finished products of powdered medicine are formed by cultivating the liquid seed, the nutritive liquid and the powder dust of pulverized coal ash for 6 days under the condition of 26 to 29 DEG C. Preventing effect is 85.2% through experiments on two kinds of different crops. The method has the advantages of easy acquirement of raw material for production, no three wastes, quick production rate, low cost, no harm of products for people and livestock and no environmental pollution, and the present invention opens up a new path for environmental protection and the comprehensive utilization of the pulverized coal ash.
Description
The present patent application relates to biopesticide insecticide processing technology, and particularly relating to a kind of is the biological bacteria pesticide preparation technology of carrier with the flyash dust.
Because chemical pesticide is on the rise to the harm that people cause, the application study of using biologic product control crop pests has been seemed particularly important.Utilize the microorganism formulation desinsection history of existing decades.The bacteria agent desinsection has the selectivity to insect, and to the person poultry harmless, environmentally safe.The production method of biological bacteria pesticide is constantly being improved always for a long time, and the most basic purpose is to make the quality of product, the requirement that cost is taken like a shot near the peasant more.The fact shows that same a kind of bacteria agent can have different processes because of raw material difference, the working condition selected for use are different, different raw materials, and meeting directly influence quality, effect and the cost of product, and the complexity of processing technology.Flyash is the discarded object of the electric enterprise of a kind of coal discharging at present, because the stacking floor space is bigger, works the mischief greatly to environment, and along with the accelerated development of city and rural industry enterprise, steam power plant's operation number constantly increases, and mainly is villages and towns in rural areas enterprise.So far China's flyash annual emissions is 8,000 ten thousand tons, according to relevant department's prediction, will reach 1.6 hundred million tons to flyash annual emissions in 2000, so the development and use of flyash is imperative.At present, flyash 35% is used to produce building material product, and 25% is the roadbed compound, and 15% is backfill, be used to improve clayed soil and the compound agricultural of making ground and the little fertilizer of paddy rice is used also just experimental stage, and be used for as biological bacteria carrier insecticide use still belong at present blank.Show that by experiment the flyash carrier does not only have negative interaction to crop, opposite existing report has the people to utilize the flyash carrier to obtain very good effect as little fertile agent of paddy rice and improvement clayed soil.
The objective of the invention is to solve the problem that exists among existing biological bacteria pesticide and the preparation technology thereof, provide a kind of flyash dust that utilizes to do the processing technology that biological bacteria pesticide is produced carrier and field carrier, production technology is more simplified, reducing production costs is beneficial to the deep popularization of biological bacteria insecticidal technology, pollutes the new approach of having opened up for solving coal electricity enterprise dust atmosphere simultaneously.
The present invention realizes with following technical scheme: the biological bacteria pesticide preparation technology who makes carrier with the flyash dust comprises the processing step of following order: (1), in the ratios preparation that adds 20 gram industrial corn powder, the industrial wheatfeed of 0.25 gram, 0.14 gram, 2000 unit Ye Huameis in the 200ml aqueous solution, transfer PH6.2-6.4 control temperature 83 ℃ ± 1, kept liquefying time 0.5 hour, obtain being no less than the liquefaction juice of reducing sugar 23.6%; (2), utilizing sabouraud medium or 20% murphy juice to add 0.5% peptone contains sugar 2% synthetic medium and slant strains or the pure white muscardine fungi powder that cultivates on the plane and millet is cultivated are carried out the liquid one grade fermemtation cultivates, utilize the liquefaction juice of step (1) preparation again, make reducing sugar fall concentration to 10% and be added with 1.0% peptone, 0.1%KH
2PO
4, 0.03%MgSO
47H
2O and 0.3% NH
4NO
3, transfer PH6.2, carried out aerobic culture 36 hours, obtain containing the seed liquor of a large amount of bacterium glueballs; (3), with the liquefaction syrup of step (1) preparation, making concentration of reduced sugar is 15-20%, and adds peptone 0.8%, NH
4NO
30.3%, industrial yeast 0.3%, transfers PH6.2, is nutrient solution; (4), mix mutually with 1: 3 ratio with the nutrient solution that step (3) makes, mixing the back is the seed nutrient mixing liquid according to the made seed liquor of step (2); (5), seed nutrient mixing liquid that step (4) is made with cross 300 purpose xeraphium coal ash dust and mix mutually with 1: 2.5 ratio, make mixture; (6), mixture cultivated through 26-29 ℃ to make finished product 1kg to drying in 6 days be the dry pulvis of 300 purposes, it is moisture less than 8%, spore content is no less than hundred million spore/grams of 80-120.
The beneficial effect that the present invention had: 1, because the present invention utilizes flyash dust carrier to produce biological bacteria pesticide, therefore have many advantages: (1), have the better physical dispersiveness by 300 purpose xeraphium coal ash dust, apparent specific gravity (gd) is 0.36-0.43, self meets the pulvis requirement.(2), flyash is the dust that reclaims behind the high-temp combustion, is no less than 30-40% by 300 purpose dust, be applied to produce do not need to add in addition handle and aseptic, therefore greatly save energy cost, reduce simultaneously and pollute, reduce production link and shorten the production cycle, reduce production costs, be easy to go into operation.(3), the pH value of flyash dust in the aqueous solution is 6.8-7.0, be neutrality, suction is close to 0% in the air of dry back, the flyash Main Ingredients and Appearance is that 63.66% silica, alundum (Al 22.03%, calcium oxide, magnesium, sodium, iron, potassium etc. respectively are 0.81-4.94%, these character show, flyash is the inertia material, the ventilative and product drying as the not only favourable thalline of carrier aborning, but also help the preservation of thalline preparation.(4), compare dust carrier escapable cost 30-160% with materials such as the present conventional Dilution for powder agent talcum powder that adopts, diatomite, potter's clay.Dust carrier cost is low and raw material is easy to get.2, the biological bacteria pesticide that utilizes the flyash dust to make as carrier has and dyes the worm effect preferably.This result can dye worm test and obtains from following: be averaged 1,000,000,000 of spore contents/former powder of gram microbial inoculum, dilution is carried out indoor bioassay for 100 times, method is as follows: a, for trying Xinxiang, worm Henan Qi Liying cotton protospecies field cotton bollworm, getting field two instars.B, establish experimental group, get the former powder of 1 gram medicament and be diluted to 100 times, test separating tests group and test repeating groups, other has control group.Every group with 30 of worm amounts.C, method are all put into the plastic bottle of pricking the hole with the test cotton earworm, and bottle is of a size of: diameter 2.5cm * high 4.5cm, put 1 cotton bollworm for every bottle, and numbering, test 1 group 1-30 number, test 2 groups 1-30 number, control group 1-30 number.Feed one every day and soak dilution 1.0 * 10
7Cotton vanelets behind individual spore/ml medicament airing, diameter 8.0mm (using the card punch cutting) observes growth and death condition every day, and control group soaks the back airing with clear water and feeds.The result is provided by table one.Table one is indoor biological test result.
Table one
| My god | Handle 1 group | Handle 2 groups | Control group |
| 3 | 0 | 0 | 0 |
| 5 | 4 | 3 | 1 |
| 7 | 18 | 24 | 5 |
| 23 | 27 | 6 |
With 1.0 * 10
7The biological test lethality that worm is dyed in individual spore/ml immersion liquid is 83.3%, corrected mortality is 79.17%.As seen dust carrier organism insecticide has the worm effect that dyes easily.Show by broccoli cabbage caterpillar test in dust carrier organism bacteria pesticide control cotton bollworm test and the control of dust carrier organism bacteria pesticide: the dust carrier is produced biological bacteria pesticide and had following advantage again: (1), dust carrier are safe from harm to crop, thalli growth there is not inhibitory action, on the contrary by the physics inertia and the excellent air permeability of dry dust, compare than other carrier and to have the thalli growth of being beneficial to and the quick-drying advantage of product.Particularly cultivate than semi-solid carriers such as rice bran, wheat brans at the critical moisture content of 30-40%, dust carrier and compare, rate of drying is fast more than 1 times, and this is to keeping the thalline activity significant.(2), production technology is easy compares with human 70% peat soil carrier production white muscardine fungi is arranged, the dust carrier need not sterilized, can directly use, in the general flyash dust collecting, reclaim the powder that falls after rise and all be not less than 93-110 ℃, this has saved energy cost for large-scale industrialization production, the operation of being convenient to found the factory.(3), dust carrier organism insecticide speed of production is fast, cost is low.Be inoculated into pulvis formulation packing and only need 6 days from cultivating carrier owing to saved energy cost and carrier cost, mu with 3kg with conventional B9
#Mu is compared with 60 grams and is saved reagent cost more than 40%.The workman bears a heavy burden and is relieved to 3kg/ mu from 30kg/ mu, has alleviated labor strength.(4), dust carrier and bacteria agent are to the person poultry harmless, environmentally safe, idol has the human body discomfort that causes to be analogous to the dry effect of mist that is caused in the excessive immersion lung of conventional dust, it is not pathology, as long as operator avoid a little with mouth mask etc. just can avoid, and can't compare the harm that people and animals and environment cause with chemical pesticide.Particularly reagent cost is low, the using method flexible and convenient is easily grasped, this is with the popularization of favourable bacteria agent insecticidal technology, particularly present insect pest wildness, the enhancing of the antagonism property of medicine causes the alternately rising with concentration and dosage, and reagent cost raises, and peasant's burden and people are occurred repeatedly by the incident of poisoning, therefore promote cheaply bacteria agent and can reduce at least and use the chemical pesticide number of times, be subjected to peasant's popular welcome surely.The rural area is wide market, the dust carrier is used to produce biological bacteria pesticide, with consumption 3kg/ mu, only corn crop area in Heilongjiang Province's can digest 300 tons of dust of daily output (crossing 300 orders by 40% calculates), 4 in steam power plant, this will make the discarded object of the electricity of coal for many years enterprise pollution become favourable agricultural production, and the beneficial products that maintaining ecological balance contributes becomes possibility, for coal electricity enterprise total utilization of PCA has been showed rosy prospect.
Description of drawings:
Accompanying drawing is the preparation of bacteriological insecticide with fly-ash as carrier flow chart.
Describe the present invention in detail below in conjunction with preparation of bacteriological insecticide with fly-ash as carrier flow chart and embodiment, the preparation technology who makes biological bacteria pesticide as carrier with the flyash dust in turn includes the following steps: at first get slant strains white muscardine fungi B9
#This bacterial classification separates wild white muscardine fungi by Jilin Academy of Agricultural Sciences or Xinxiang, Henan cotton protospecies field to be provided, getting bacterial classification 2-3 encircles in the 250ml triangular flask and filled in the 50ml sabouraud medium one-level kind liquid shaken cultivation 24 hours, 26 ℃ ± 1 ° of temperature, microscopy mycelia spore does not completely have assorted bacterium and changes secondary cultivation over to 1: 10 ratio, in the inferior one-level culture fluid reducing sugar be 5%, peptone 1.0%, KH
2PO
40.1%, MgSO
47H
2O0.03%, NH
4NO
30.3%, other condition is cultivated with one-level.Microscopy normally changes in the seeding tank with 1.5: 10 ratios in sterile working after 30 hours, seeding tank 110 liters, dress seed liquor 70 liters, corn flour 20 restrains to add in the 200ml aqueous solution, wheatfeed 0.25 restrains by containing, 2000 unit Ye Huameis, 0.14 gram proportioning is made, at 83 ℃ ± 1 ° 0.5 hour liquefaction corn steep liquor, reducing sugar 24.6% transfers to 5.0%, PH:6.2, peptone 0.8%, KH
2PO
40.1%, MgSO
47H
2O0.03% ventilated 1: 1.5, revolution 160R/min, and 26 ± 1 ℃ of temperature in time go out jar because the fluctuation of pH value is paid close attention in the 36-40 hour seed liquor in fermentation back when mycelia forms a large amount of bacterium glueballs.Seed liquor goes out a jar back mixes with the nutrition liquid phase with 1: 3 ratio, and nutrient solution is with the same liquefaction corn steep liquor of seed liquor, contains reducing sugar 15%, peptone 0.8%, dusty yeast 0.3%, PH6.2.Seed liquor, nutrient solution is mixed and afterwards mixes mutually with 1: 3: 10 ratio with mistake 300 order xeraphium coal ash, at capacity is to be advisable to loose no lump in the horizontal sealing agitator tank of 240kg, going out jar is placed on 24-26 ℃ of aeration-drying training between cultivating and puts heat insulating culture on the frame, 10 layers on frame is put in training, interval 10cm, put 90 * 50 * 5cm for every layer two of grid treuchers are arranged, every dish blowing 10kg, three days with Polypropylence Sheet frame being put in training fences up, recession in three days is the Polypropylence Sheet and the 26-29 ℃ of cultivation 3 days that heat up down, again the charging tray microbial inoculum is moved in the horizontal stirring tank and stir, leak outside, feed 32 ± 1 ℃ of extremely whole dry no particle powders that are of hot blast to reduce dust.Kilogram surplus employing the method is produced seven batches about 2600 altogether, spore content 80-120 hundred million/gram.
Product quality detection method: 1, the mensuration of sample miospore number: (1), sampling: from every batch sample, extract 10 parts, every part of sample thief dust culture 5 grams, the back that is mixed accurately takes by weighing 5 grams with 1% analytical balance and tests twice of repeated sampling.(2), checked operation: will detect sample and put into the aseptic tween liquid that the 300ml triangular flask contains 100ml0.1%, with 160 rev/mins of magnetic stirring apparatuss stir 30 minutes also the useable glass pearl on oscillator, make of 30 minutes, drawing equal slurries 10ml with suction pipe adds in the aseptic tween liquid of 90ml0.1%, magnetic agitation 30 minutes, drawing 10ml again adds in the aseptic tween liquid of 90ml0.1%, stirred 30 minutes, this moment, diluted sample was 2000 times.(3), counting: with the dilution of inhaling battalion's draw dust culture, get the blood counting chamber that cleans up, build the special cap slide sample liquid is splashed into a little gently by edge, cover glass middle part, not flow into groove degree of being.With tally on dressing table static 2 minutes, the spore that flows that suspends is settled out, at 600 times is 40 * 15 microscopicallies countings, tally with 25 big lattice (being the two-wire lattice) * 16 little lattice (being the single line lattice), then calculating in the big lattice of big lattice and central authorities at four angles of periphery, totally 80 lattice spore counts are hundred million numbers, if the tally of 16 big grid * 25 lattices then only calculates the big grid at four angles of periphery, be calculated as follows:
2, the spore rate of living is that germination rate is measured: (1), medium preparation: get 20% murphy juice 100ml with sucrose 2 grams, white peptone 1 gram of dawn, transfer PH5.5, be sub-packed in each 50ml of 250ml triangular flask, add bead 5-10 grain, add tampon and brown paper system mouth, through high pressure (1kg/cm
2) sterilized 30 minutes, cool off standby.(2), connecing bacterium cultivates: get dust culture 2 grams and add in the 200ml0.1% tween sterile diluent, fully stirred 30 minutes, get in the medium that dilution 0.5ml adds the sterilization cooling, build tampon and be mouthful to be placed on 24-26 ℃ of shaken cultivation 20-24 hour, more than should be in aseptic operation down.(3), microscopy counting: culture is taken out the back carries out the non-quantitative dilution, see 30-40 spore degree of being with every visual field under 600 power microscopes with sterile water, add up each visual field germinating spore number and not germinating spore to count summation be 100.Each sample is done 3 repetitions, is calculated as follows:
3, sample moisture determination
Marine products SC69-02 type water tester in the employing is at first adjusted reading zero point with 5 gram sample weights, takes off the sample weights, put into sample to zero point and locate, shut the window that feeds intake, open heating power supply, sample drying to constant weight was kept two hours, and this moment, reading value was the water content value.Table two has provided the index of product quality.Method of testing is formulated white muscardine fungi with reference to Hu'nan Prov. Academy of Forest-Sciences and is produced and the product quality examination criteria.
Table two
| Hundred million/gram of spore content | Germination rate % | Cross 300 mesh sieve % | Moisture % | Apparent specific gravity (gd) | |
| Technical indicator | 8．0×10 ～1．2×10 | ＞85 | ＞98 | ＜8％ | 0．32 ～ 0．45 |
Above preparation technology improves a little equally also can produce thuricade-1 and other biological bacteria preparation.Therefore be the method for biological bacteria preparation of the carrier institute processing of the material scope of patent protection of the present invention of all can yet be regarded as with flyash dust and inert dust, powder.
Claims (1)
1, a kind of preparation of bacteriological insecticide with fly-ash as carrier is characterized in that comprising successively the processing step of following order:
(1), in the ratios preparation that adds 20 gram industrial corn powder, the industrial wheatfeed of 0.25 gram, 0.14 gram, 2000 unit Ye Huameis in the 200ml aqueous solution, transfer PH6.2-6.4 control temperature 83 ℃ ± 1, kept liquefying time 0.5 hour, obtain being no less than the liquefaction juice of reducing sugar 23.6%;
(2), utilizing sabouraud medium or 20% murphy juice to add 0.5% peptone contains sugar 2% synthetic medium and slant strains or the pure white muscardine fungi powder that cultivates on the plane and millet is cultivated are carried out the liquid one grade fermemtation cultivates, utilize the liquefaction juice of step (1) preparation again, make reducing sugar fall concentration to 10% and be added with 1.0% peptone, 0.1%KH
2PO
4, 0.03%MgSO
47H
2O and 0.3% NH
4NO
3, transfer PH6.2, carried out aerobic culture 36 hours, obtain containing the seed liquor of a large amount of bacterium glueballs;
(3), with the liquefaction syrup of step (1) preparation, making concentration of reduced sugar is 15-20%, and adds peptone 0.8%, NH
4NO
30.3%, industrial yeast 0.3%, and accent PH6.2 is a nutrient solution;
(4), mix mutually with 1: 3 ratio with the nutrient solution that step (3) makes, be mixed into the seed nutrient mixing liquid according to the made seed liquor of step (2);
(5), seed nutrient mixing liquid that step (4) is made with cross 300 purpose xeraphium coal ash dust and mix mutually with 1: 2.5 ratio, make mixture;
(6), mixture cultivated through 26-29 ℃ to make finished product 1kg to drying in 6 days be the dry pulvis of 300 purposes, it is moisture less than 8%, spore content is no less than hundred million spore/grams of 80-120.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97118839A CN1065111C (en) | 1997-10-21 | 1997-10-21 | Preparation of bacteriological insecticide with fly-ash as carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97118839A CN1065111C (en) | 1997-10-21 | 1997-10-21 | Preparation of bacteriological insecticide with fly-ash as carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1178071A CN1178071A (en) | 1998-04-08 |
| CN1065111C true CN1065111C (en) | 2001-05-02 |
Family
ID=5175002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97118839A Expired - Fee Related CN1065111C (en) | 1997-10-21 | 1997-10-21 | Preparation of bacteriological insecticide with fly-ash as carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1065111C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105360167A (en) * | 2015-09-26 | 2016-03-02 | 黄山学院 | Density-board-based slow-released biological prevention and control agent and preparing method and application thereof |
| CN105230665A (en) * | 2015-09-26 | 2016-01-13 | 黄山学院 | Wax paper based sustain-released bio-control agent, preparation method and applications thereof |
| CN105340882A (en) * | 2015-12-11 | 2016-02-24 | 江苏仁信作物保护技术有限公司 | Application of coal ash serving as pesticide powder filler |
| CN105875653B (en) * | 2016-04-15 | 2018-08-17 | 佛山市聚成生化技术研发有限公司 | A kind of beauveria bassiana microbial pesticide and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100658A (en) * | 1986-01-21 | 1987-10-07 | 云南省玉溪市北山林场 | The solid culture method of white muscardine fungi |
| CN1072311A (en) * | 1992-10-21 | 1993-05-26 | 那福延 | A kind of insecticide carrier |
| CN1085733A (en) * | 1992-08-19 | 1994-04-27 | 生态科学公司 | The biological control of termite |
| JPH06305907A (en) * | 1993-04-28 | 1994-11-01 | Shikoku Sogo Kenkyusho:Kk | Noxious insect repelling method |
-
1997
- 1997-10-21 CN CN97118839A patent/CN1065111C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100658A (en) * | 1986-01-21 | 1987-10-07 | 云南省玉溪市北山林场 | The solid culture method of white muscardine fungi |
| CN1085733A (en) * | 1992-08-19 | 1994-04-27 | 生态科学公司 | The biological control of termite |
| CN1072311A (en) * | 1992-10-21 | 1993-05-26 | 那福延 | A kind of insecticide carrier |
| JPH06305907A (en) * | 1993-04-28 | 1994-11-01 | Shikoku Sogo Kenkyusho:Kk | Noxious insect repelling method |
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| Publication number | Publication date |
|---|---|
| CN1178071A (en) | 1998-04-08 |
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