CN101387648A - 红细胞膜抗原磁珠试剂盒及在血型抗体检测方面的应用 - Google Patents
红细胞膜抗原磁珠试剂盒及在血型抗体检测方面的应用 Download PDFInfo
- Publication number
- CN101387648A CN101387648A CN200810051319.1A CN200810051319A CN101387648A CN 101387648 A CN101387648 A CN 101387648A CN 200810051319 A CN200810051319 A CN 200810051319A CN 101387648 A CN101387648 A CN 101387648A
- Authority
- CN
- China
- Prior art keywords
- membrane antigen
- magnetic bead
- erythrocyte membrane
- antibody
- blood group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 54
- 239000008280 blood Substances 0.000 title claims abstract description 54
- 101710181478 Envelope glycoprotein GP350 Proteins 0.000 title claims abstract description 34
- 210000003617 erythrocyte membrane Anatomy 0.000 title claims description 26
- 238000001514 detection method Methods 0.000 title claims description 14
- 239000011324 bead Substances 0.000 claims abstract description 35
- 239000000427 antigen Substances 0.000 claims abstract description 25
- 102000036639 antigens Human genes 0.000 claims abstract description 25
- 108091007433 antigens Proteins 0.000 claims abstract description 25
- 238000006243 chemical reaction Methods 0.000 claims abstract description 22
- 210000004027 cell Anatomy 0.000 claims abstract description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000010790 dilution Methods 0.000 claims description 9
- 239000012895 dilution Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 8
- 239000002608 ionic liquid Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 3
- 229940098773 bovine serum albumin Drugs 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 235000013905 glycine and its sodium salt Nutrition 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 210000000170 cell membrane Anatomy 0.000 abstract description 8
- 238000012360 testing method Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000003085 diluting agent Substances 0.000 abstract 1
- 210000003743 erythrocyte Anatomy 0.000 description 29
- 238000012216 screening Methods 0.000 description 18
- 210000002966 serum Anatomy 0.000 description 15
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 230000001788 irregular Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 102000006395 Globulins Human genes 0.000 description 5
- 108010044091 Globulins Proteins 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 230000004523 agglutinating effect Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000006249 magnetic particle Substances 0.000 description 3
- 230000005415 magnetization Effects 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 101000767750 Carya illinoinensis Vicilin Car i 2.0101 Proteins 0.000 description 2
- 101000767759 Corylus avellana Vicilin Cor a 11.0101 Proteins 0.000 description 2
- 101000622316 Juglans regia Vicilin Jug r 2.0101 Proteins 0.000 description 2
- 101000767757 Pinus koraiensis Vicilin Pin k 2.0101 Proteins 0.000 description 2
- 101000767758 Pistacia vera Vicilin Pis v 3.0101 Proteins 0.000 description 2
- 208000003441 Transfusion reaction Diseases 0.000 description 2
- 238000009582 blood typing Methods 0.000 description 2
- 239000012267 brine Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000002949 hemolytic effect Effects 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 206010018833 Haematocoele Diseases 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 208000005873 Hematocele Diseases 0.000 description 1
- 241001662043 Icterus Species 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241000406668 Loxodonta cyclotis Species 0.000 description 1
- GCKMFJBGXUYNAG-HLXURNFRSA-N Methyltestosterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@](C)(O)[C@@]1(C)CC2 GCKMFJBGXUYNAG-HLXURNFRSA-N 0.000 description 1
- 208000001300 Perinatal Death Diseases 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 201000004920 hematocele of tunica vaginalis testis Diseases 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 229940085503 testred Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200810051319.1A CN101387648A (zh) | 2008-10-24 | 2008-10-24 | 红细胞膜抗原磁珠试剂盒及在血型抗体检测方面的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200810051319.1A CN101387648A (zh) | 2008-10-24 | 2008-10-24 | 红细胞膜抗原磁珠试剂盒及在血型抗体检测方面的应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN101387648A true CN101387648A (zh) | 2009-03-18 |
Family
ID=40477188
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200810051319.1A Pending CN101387648A (zh) | 2008-10-24 | 2008-10-24 | 红细胞膜抗原磁珠试剂盒及在血型抗体检测方面的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101387648A (zh) |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102175877A (zh) * | 2010-12-31 | 2011-09-07 | 周胜利 | 一种微量,快速和精确检测人红细胞抗原和血清中抗体的方法 |
CN102338800A (zh) * | 2011-05-25 | 2012-02-01 | 上海血液生物医药有限责任公司 | 抗体筛选红细胞试剂盒 |
CN102692510A (zh) * | 2012-06-07 | 2012-09-26 | 北京金豪制药股份有限公司 | 广谱抗人球蛋白试剂检测卡及其制备 |
CN102879560A (zh) * | 2012-10-22 | 2013-01-16 | 周胜利 | 一种精确、快速和微量检测人血清中抗原和抗体的方法 |
CN103344755A (zh) * | 2013-07-19 | 2013-10-09 | 中国科学院苏州生物医学工程技术研究所 | 一种磁化醛化羊红细胞的制备方法 |
CN103487590A (zh) * | 2013-09-03 | 2014-01-01 | 华南农业大学 | 一种血型抗体的间接Dot-ELISA检测方法与应用 |
CN104569376A (zh) * | 2013-10-09 | 2015-04-29 | 北京康华源科技发展有限公司 | 一种磁微粒介导的层析检测技术装置及其用途 |
CN104569400A (zh) * | 2014-12-05 | 2015-04-29 | 英科新创(厦门)科技有限公司 | 一种不完全抗体筛查胶体金试剂盒及其制备方法 |
CN104749152A (zh) * | 2015-04-15 | 2015-07-01 | 哈尔滨医科大学 | 肺癌循环肿瘤细胞检测装置 |
EP3165920A1 (de) * | 2015-11-06 | 2017-05-10 | imusyn GmbH & Co. KG | Verfahren zur analyse von serum auf anti-erythrozytäre antikörper |
CN107643409A (zh) * | 2017-09-19 | 2018-01-30 | 汪德清 | 一种血型抗原芯片及其在红细胞意外抗体检测中的应用 |
CN114397464A (zh) * | 2022-03-24 | 2022-04-26 | 天津德祥生物技术有限公司 | 一种红细胞膜碎片与载体的偶联复合物、其偶联方法和应用 |
-
2008
- 2008-10-24 CN CN200810051319.1A patent/CN101387648A/zh active Pending
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102175877A (zh) * | 2010-12-31 | 2011-09-07 | 周胜利 | 一种微量,快速和精确检测人红细胞抗原和血清中抗体的方法 |
CN102338800A (zh) * | 2011-05-25 | 2012-02-01 | 上海血液生物医药有限责任公司 | 抗体筛选红细胞试剂盒 |
CN102692510A (zh) * | 2012-06-07 | 2012-09-26 | 北京金豪制药股份有限公司 | 广谱抗人球蛋白试剂检测卡及其制备 |
CN102879560A (zh) * | 2012-10-22 | 2013-01-16 | 周胜利 | 一种精确、快速和微量检测人血清中抗原和抗体的方法 |
CN103344755B (zh) * | 2013-07-19 | 2015-06-24 | 中国科学院苏州生物医学工程技术研究所 | 一种磁化醛化羊红细胞的制备方法 |
CN103344755A (zh) * | 2013-07-19 | 2013-10-09 | 中国科学院苏州生物医学工程技术研究所 | 一种磁化醛化羊红细胞的制备方法 |
CN103487590A (zh) * | 2013-09-03 | 2014-01-01 | 华南农业大学 | 一种血型抗体的间接Dot-ELISA检测方法与应用 |
CN103487590B (zh) * | 2013-09-03 | 2015-04-08 | 华南农业大学 | 一种血型抗体的间接Dot-ELISA检测方法与应用 |
CN104569376A (zh) * | 2013-10-09 | 2015-04-29 | 北京康华源科技发展有限公司 | 一种磁微粒介导的层析检测技术装置及其用途 |
CN104569400A (zh) * | 2014-12-05 | 2015-04-29 | 英科新创(厦门)科技有限公司 | 一种不完全抗体筛查胶体金试剂盒及其制备方法 |
CN104749152A (zh) * | 2015-04-15 | 2015-07-01 | 哈尔滨医科大学 | 肺癌循环肿瘤细胞检测装置 |
CN104749152B (zh) * | 2015-04-15 | 2018-04-27 | 哈尔滨医科大学 | 肺癌循环肿瘤细胞检测装置 |
EP3165920A1 (de) * | 2015-11-06 | 2017-05-10 | imusyn GmbH & Co. KG | Verfahren zur analyse von serum auf anti-erythrozytäre antikörper |
CN107643409A (zh) * | 2017-09-19 | 2018-01-30 | 汪德清 | 一种血型抗原芯片及其在红细胞意外抗体检测中的应用 |
WO2019056673A1 (zh) * | 2017-09-19 | 2019-03-28 | 北京三样生物科技有限公司 | 一种血型抗原芯片及其在红细胞意外抗体检测中的应用 |
WO2019057014A1 (zh) * | 2017-09-19 | 2019-03-28 | 汪德清 | 一种血型抗原芯片及其在红细胞意外抗体检测中的应用 |
CN114397464A (zh) * | 2022-03-24 | 2022-04-26 | 天津德祥生物技术有限公司 | 一种红细胞膜碎片与载体的偶联复合物、其偶联方法和应用 |
CN114397464B (zh) * | 2022-03-24 | 2022-06-10 | 天津德祥生物技术有限公司 | 一种红细胞膜碎片与载体的偶联复合物、其偶联方法和应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101387648A (zh) | 红细胞膜抗原磁珠试剂盒及在血型抗体检测方面的应用 | |
Decary et al. | An investigation of nonhemolytic transfusion reactions | |
AU2002248996B2 (en) | Detection of candida | |
McPhaul Jr et al. | The presence of anti-glomerular basement membrane antibodies in peripheral blood | |
CN101315386A (zh) | 高密度介质固相抗人球蛋白试剂盒 | |
CN107643409A (zh) | 一种血型抗原芯片及其在红细胞意外抗体检测中的应用 | |
CN101109756A (zh) | 一种用于检测血清中不规则抗体的试剂盒及其制备方法 | |
CN108680756A (zh) | 一种不完全抗体检测试剂盒及检测方法 | |
CN102435756A (zh) | ABO、RhD血型定型检测卡的制备方法 | |
CN102175877A (zh) | 一种微量,快速和精确检测人红细胞抗原和血清中抗体的方法 | |
CN110520736A (zh) | 一种功能化生物多层孔隙膜免疫载体、制备方法、应用 | |
CN102507961B (zh) | 新生儿ABO/Rh血型鉴定试剂卡及其制备方法 | |
CN107022527A (zh) | 可分泌抗c反应蛋白单克隆抗体的杂交瘤细胞、c反应蛋白检测试剂及其制备方法和应用 | |
CN101718784B (zh) | 新生儿溶血病检测试剂盒的制备方法 | |
CN101109755A (zh) | 一种用于筛查血清中不规则抗体的试剂盒及其制备方法 | |
IE42031B1 (en) | Detection of hepatitis b surface antigen by latex agglutination | |
CN113156143A (zh) | 血型不规则抗体特异性鉴定方法及其试剂 | |
Fritz et al. | Hepatitis-associated antigen: detection by antibody-sensitized latex particles | |
CN112698043A (zh) | 一种血小板抗体检测试剂盒、应用及检测方法 | |
CN105486871B (zh) | 一种快速检测犬细小病毒抗体血凝抑制效价的胶体金试纸条、试剂盒及检测方法 | |
CN101706508A (zh) | Abo亚型检定试剂盒的制备方法 | |
Steward et al. | Circulating antigen-antibody complexes in onchocerciasis. | |
CN108548921A (zh) | 一种间接抗人球蛋白加速配血的试验方法 | |
Lau et al. | Positive direct antiglobulin reaction in a patient population | |
EP0022669B1 (en) | Method and reagent for blood typing and method for preparing this reagent |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: CHANGCHUN BLOOD BIO-TECH CO., LTD. Free format text: FORMER OWNER: LI YONG Effective date: 20110707 |
|
C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 130012 NO. 433, CHENGUANG STREET, HIGH-TECH. DEVELOPMENT ZONE, CHANGCHUN CITY, JILIN PROVINCE TO: 130012 NO. 3255, QIANJIN STREET, CHANGCHUN CITY, JILIN PROVINCE |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20110707 Address after: 130012 No. 3255 Qianjin Street, Jilin, Changchun Applicant after: Changchun Brother Biotech Co., Ltd. Address before: 130012 No. 433 Chenguang street, hi tech Development Zone, Jilin, Changchun Applicant before: Li Yong |
|
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20090318 |