CN101358180B - 一种由雷公藤细胞培养法生产雷公藤甲素和生物碱的方法 - Google Patents
一种由雷公藤细胞培养法生产雷公藤甲素和生物碱的方法 Download PDFInfo
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- CN101358180B CN101358180B CN2008101507094A CN200810150709A CN101358180B CN 101358180 B CN101358180 B CN 101358180B CN 2008101507094 A CN2008101507094 A CN 2008101507094A CN 200810150709 A CN200810150709 A CN 200810150709A CN 101358180 B CN101358180 B CN 101358180B
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生长培养基(mg/L) | 生产雷公藤甲素培养基(mg/L) | 生产雷公藤生物碱培养基(mg/L) | |
NH<sub>4</sub>NO<sub>3</sub> | 1650 | 825 | - |
生长培养基(mg/L) | 生产雷公藤甲素培养基(mg/L) | 生产雷公藤生物碱培养基(mg/L) | |
KNO<sub>3</sub> | 1900 | 950 | - |
(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> | - | - | 3960 |
KCl | - | - | 745 |
MgSO<sub>4</sub>·7H<sub>2</sub>O | 1233 | 1233 | 1233 |
KH<sub>2</sub>PO<sub>4</sub> | 1360 | 1360 | 680 |
CaCl<sub>2</sub>·2H<sub>2</sub>O | 440 | 147 | 220 |
FeSO<sub>4</sub>·7H<sub>2</sub>O | 27.85 | 9.28 | 9.28 |
Na<sub>2</sub>EDTA·2H<sub>2</sub>O | 37.25 | 12.42 | 12.42 |
MnSO<sub>4</sub>·7H<sub>2</sub>O | 33.8 | 67.6 | 67.6 |
ZnSO<sub>4</sub>·2H<sub>2</sub>O | 8.6 | 17.2 | 34.4 |
H<sub>3</sub>BO<sub>3</sub> | 6.2 | - | - |
KI | - | 3.32 | - |
Na<sub>2</sub>MoO<sub>4</sub>·2H<sub>2</sub>O | 1 | - | - |
CoSO<sub>4</sub>·7H<sub>2</sub>O | - | - | 0.05 |
CuSO<sub>4</sub>·5H<sub>2</sub>O | 0.025 | 0.05 | 0.05 |
肌醇 | 100 | 200 | 100 |
甘氨酸 | 5 | 5 | 1 |
盐酸硫胺素 | 1 | 1 | 1 |
盐酸吡哆醇 | 1 | 1 | 1 |
烟酸 | 1 | 0.5 | - |
生物素 | - | 0.5 | 1 |
蔗糖 | 40 | 20 | - |
生长培养基(mg/L) | 生产雷公藤甲素培养基(mg/L) | 生产雷公藤生物碱培养基(mg/L) | |
葡萄糖 | - | - | 20 |
2,4-D | 1 | - | 1 |
NAA | - | 4 | - |
6-BA | - | 0.5 | - |
KT | 0.5 | - | 0.5 |
苹果炭疽病菌菌丝体 | - | 100 | 200 |
pH | 5.8 | 6.4 | 5.2 |
供试样品 | 回归方程 | 致死中浓(mg/mL) | LC<sub>50</sub>95%置信限(mg/mL) | 相关系数r |
雷公藤根皮粉 | y=3.6865+1.1747x | 13.1280 | 8.392~20.538 | 0.9844 |
悬浮细胞培养物 | y=3.6811+1.3931x | 8.8461 | 6.109~12.810 | 0.9880 |
培养液 | y=3.5677+1.2344x | 14.4634 | 9.526~21.961 | 0.9833 |
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CN2008101507094A CN101358180B (zh) | 2008-08-25 | 2008-08-25 | 一种由雷公藤细胞培养法生产雷公藤甲素和生物碱的方法 |
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CN2008101507094A CN101358180B (zh) | 2008-08-25 | 2008-08-25 | 一种由雷公藤细胞培养法生产雷公藤甲素和生物碱的方法 |
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CN101358180A CN101358180A (zh) | 2009-02-04 |
CN101358180B true CN101358180B (zh) | 2010-09-08 |
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CN2008101507094A Expired - Fee Related CN101358180B (zh) | 2008-08-25 | 2008-08-25 | 一种由雷公藤细胞培养法生产雷公藤甲素和生物碱的方法 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2952072B1 (fr) | 2009-11-05 | 2013-09-27 | Pf Medicament | Procede de production de triptolide |
CN102807974A (zh) * | 2012-08-10 | 2012-12-05 | 福建农林大学 | 一种雷公藤细胞固定化的构建方法 |
CN103159822B (zh) * | 2013-03-04 | 2016-05-18 | 华侨大学 | 雷公藤甲素及其衍生物组合物的制备方法 |
CN114642171B (zh) * | 2022-03-30 | 2023-04-25 | 华侨大学 | 一种高活性雷公藤代谢产物的诱导方法及雷公藤活性代谢组合物 |
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