CN101328086B - Edible fungus cultivation technology using bean dreg liquid and preparation method thereof - Google Patents
Edible fungus cultivation technology using bean dreg liquid and preparation method thereof Download PDFInfo
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- CN101328086B CN101328086B CN2007101448585A CN200710144858A CN101328086B CN 101328086 B CN101328086 B CN 101328086B CN 2007101448585 A CN2007101448585 A CN 2007101448585A CN 200710144858 A CN200710144858 A CN 200710144858A CN 101328086 B CN101328086 B CN 101328086B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention provides a method for liquidly culturing edible fungus mycelia using soybean residue as main material. The different edible fungus mycelia are liquidly cultured by adding different ratio of sucrose or glucose, different ratio of magnesium salt, phosphate, calcium salt and ferric salt into the main materials of soybean residue immersion liquid and soybean residue. The method is suitable for liquidly culturing most edible funguses and has advantages of big density of mycelial pellet, easy separation, high biological conversion rate, low material cost, simple technology, easy industrialization. The produced edible fungus mycelia can be used in producing healthy food and drug such as edible healthy soybean powder, edible fungus polysaccharide preparation, edible healthy drink and the like.
Description
Affiliated technical field
The present invention relates to a kind of liquid nutrient medium of cultivating edible mushrooms and preparation method thereof.
Background technology
Edible mushrooms is a kind of important Biological resources, contains the rich functions biological substance, and the liquid culture edible fungus mycelium is current and even the important means of pharmaceutical industry from now on and healthcare products industry acquisition edible mushrooms functional living being material.At present, edible fungus liquid is cultivated the main meticulous raw materials such as bean powder, Semen Maydis powder, yeast extract paste, glucose that adopt, the raw materials cost height, edible mushrooms mainly utilizes low sugar, Mierocrystalline cellulose and hemicellulose in process of growth, bean powder, Semen Maydis powder can not be utilized fully after cultivating, and biological transformation ratio is low, is a kind of waste to grain resource, and optimum carbon source of bean powder, the not all edible mushrooms of Semen Maydis powder and nitrogenous source, the suitability for industrialized production of restriction edible medicinal fungus.
Bean dregs are tankage～(waste materials) that soymilk, bean powder are produced, and nutritious, output is big, produces 1 ton of bean powder, will produce 1.2 tons wet bean dregs, and at present mainly as feed, the soybean nutritional composition can not make full use of, and causes the wasting of resources.
Summary of the invention
The invention provides a kind of bean dregs liquid nutrient medium of cultivating edible mushrooms and preparation method thereof, not only cultivate the raw material resources that suitability for industrialized production searches out a kind of cheapness for edible fungus liquid, the utilization again for bean dregs simultaneously provides an effective way.
Because the contained soybean fibers of bean dregs has higher water-absorbent, the bean dregs of water content 87～90% do not have flowability, are difficult to carry out liquid fermenting.For solving this technical problem, the present invention adopts bean dregs to soak juice and directly add the method that bean dregs combine and prepare substratum.Promptly in bean dregs, add 1～2 times of hot water, regulate pH6.8～8.0, stir, filter, collect filtrate, regulate filtrate pH5.8～6.5 with citric acid again, replace water preparation substratum with this filtrate with sodium bicarbonate.
Different edible mushroomss utilizes the degree difference to Mierocrystalline cellulose, low sugar and inorganic salt etc., with the bean dregs immersion liquid is basic components, by adding the bean dregs of different ratios, different ratios auxiliary material (sucrose or glucose), the inorganic salt of suitable proportion (sal epsom, dipotassium hydrogen phosphate, potassium primary phosphate, calcium chloride, molysite) etc. can carry out the cultivation of different edible fungus myceliums.
With this substratum that contains bean dregs solvend and soybean fibers, cultivate edible mushrooms, mycelium pellet density is big, can reach 0.30～0.50g/mL, and the biological transformation ratio height can reach 70～90%, and raw materials cost is low, and technology is simple, industrialization easily.Made full use of the utilized composition of bean dregs simultaneously, improved the utilization ratio of soybean, the edible fungus mycelium that present method is cultivated can be used for producing protective foods and medicines such as edible mushrooms health care bean powder, edible mushrooms nourishing drink, edible fungi polysaccharide preparation.
Embodiment
(1) select materials: the raw material that is adopted, auxiliary material should reach food grade.Bean dregs should fresh, free from extraneous odour, inclusion-free, has the distinctive smell of soybean, the milky white or the milk yellow that are the bean dregs nature, (2) processing of bean dregs: get a certain amount of bean dregs, add 1～2 times 60～90 ℃ hot water, regulate pH6.8～8.0 with sodium bicarbonate, stir, filter, collect filtrate, regulate filtrate pH5.8～6.5 with citric acid again.(3) batching: in the bean dregs immersion liquid, add 2～20% bean dregs, 2～6% glucose or sucrose, 0.1～0.5% magnesium salts, 0.1～0.8% phosphoric acid salt, 0.1～0.5% calcium salt, 0.01～0.1 ‰ molysite.(4) sterilization and cooling: adopt 100～120 ℃, real jar of sterilization of 10～60min is cooled to 25～30 ℃ of inoculation temps then.(5) inoculation: aseptic technique, insert the edible fungus liquid kind by 5～15%.(6) fermentation: at ventilating ratio 1: 0.1～1.0, revolution is 100～200r/min, carries out fermentation culture under 25～30 ℃ the condition and gets final product in 6～12 days.
Description of drawings
Accompanying drawing is a bean dregs liquid culture edible mushrooms process flow sheet.
Claims (2)
1. bean dregs liquid nutrient medium of cultivating edible mushrooms, the bean dregs liquid nutrient medium that it is characterized in that cultivating edible mushrooms is a major ingredient with bean dregs immersion liquid and bean dregs, auxiliary material is magnesium salts, phosphoric acid salt, calcium salt, molysite and glucose or sucrose, and its medium component and weight percentage are as follows: 2～20% bean dregs, 2～6% glucose or sucrose, 0.1～0.5% magnesium salts, 0.1～0.8% phosphoric acid salt, 0.1～0.5% calcium salt, 0.01～0.1%o molysite and 60～99% bean dregs immersion liquid; Wherein the bean dregs immersion liquid is to get a certain amount of wet bean dregs, adds 60～90 ℃ of hot water of 1～2 times, regulates pH6.8～8.0 with sodium bicarbonate then, stirs, and filters, and collects filtrate, regulates filtrate pH5.8～6.5 with citric acid again and makes.
2. cultivate the preparation method of the bean dregs liquid nutrient medium of edible mushrooms according to claim 1, it is characterized in that the bean dregs liquid nutrient medium of cultivating edible mushrooms prepares according to the following steps:
One, select materials: the raw material that is adopted, auxiliary material should reach food grade, and bean dregs should fresh, free from extraneous odour, inclusion-free, have the distinctive smell of soybean, be the milky white of bean dregs nature or the bean dregs of milk yellow;
Two, preparation bean dregs immersion liquid: get a certain amount of wet bean dregs, add 60～90 ℃ of hot water of 1～2 times, regulate pH6.8～8.0, stir, filter, collect filtrate, regulate filtrate pH5.8～6.5 with citric acid again with sodium bicarbonate;
Three, percentage composition is prepared the bean dregs immersion liquid of 2～20% bean dregs, 2～6% glucose or sucrose, 0.1～0.5% magnesium salts, 0.1～0.8% phosphoric acid salt, 0.1～0.5% calcium salt, 0.01～0.1 ‰ molysite and 60～99% by weight;
Four, sterilization and cooling: adopt 100～120 ℃, real jar of sterilization of 10～60min, be cooled to 25～30 ℃ of inoculation temps then;
Five, inoculation: aseptic technique, insert the edible fungus liquid kind by 5～15%;
Six, fermentation: at ventilating ratio 1: 0.1～1.0, revolution is 100～200r/min, carries out fermentation culture under 25～30 ℃ the condition 6～12 days;
Promptly obtain to cultivate the bean dregs liquid nutrient medium of edible mushrooms.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007101448585A CN101328086B (en) | 2007-12-19 | 2007-12-19 | Edible fungus cultivation technology using bean dreg liquid and preparation method thereof |
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| CN2007101448585A CN101328086B (en) | 2007-12-19 | 2007-12-19 | Edible fungus cultivation technology using bean dreg liquid and preparation method thereof |
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| CN101328086B true CN101328086B (en) | 2010-11-17 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101797497A (en) * | 2010-03-26 | 2010-08-11 | 沈阳化工学院 | Mould biosorbent and preparation method thereof |
| CN102119741B (en) * | 2011-01-19 | 2012-12-12 | 佳木斯大学 | Edible fungi soy milk, edible fungi sugar-free soy flour and preparation method thereof |
| CN104664041A (en) * | 2015-03-27 | 2015-06-03 | 湖南威斯珈生物科技有限公司 | Edible biological compound protein powder and preparation method thereof |
| CN104718993A (en) * | 2015-03-31 | 2015-06-24 | 福建嘉田农业开发有限公司 | Liquefying inoculation method for edible and medicinal fungi using bean dregs as raw material |
| CN104824168A (en) * | 2015-04-28 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Preparation technology and drinking method of edible fungus finely-grinded bean powder |
| CN104877912A (en) * | 2015-05-11 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Liquid fermentation culture medium for leucopaxillus amarus kuehn and preparation method of liquid fermentation culture medium |
| CN106036810A (en) * | 2016-06-14 | 2016-10-26 | 安徽师范大学 | Preparation method of Stropharia rugosoannulata edible fungus flavor bean residue food raw material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1699305A (en) * | 2005-05-19 | 2005-11-23 | 蒋文兰 | Water-preserving nutrient soil for indoor flowers |
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| CN1699305A (en) * | 2005-05-19 | 2005-11-23 | 蒋文兰 | Water-preserving nutrient soil for indoor flowers |
Non-Patent Citations (1)
| Title |
|---|
| 汪尚法等.玉米芯豆腐渣混合培养基的平菇栽培技术.中国农村科技.2005,(3), * |
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Inventor after: Ding Yuping Inventor after: Han Hua Inventor after: Han Chengwu Inventor after: Zhao Yongxun Inventor after: Jing Guiyun Inventor after: Shen Jian Inventor after: Yong Daming Inventor before: Ding Yuping Inventor before: Zhao Yongxun Inventor before: Jing Guiyun Inventor before: Yong Daming Inventor before: Shen Jian |
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Free format text: CORRECT: INVENTOR; FROM: DING YUPING ZHAO YONGXUN JING GUIYUN YONG DAMING SHEN JIAN TO: DING YUPINGHAN HUA HAN CHENGWU ZHAO YONGXUN JING GUIYUN SHEN JIAN YONG DAMING |
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