CN106148204A - A kind of Ganoderma liquid fermentation medium and preparation method thereof - Google Patents
A kind of Ganoderma liquid fermentation medium and preparation method thereof Download PDFInfo
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- CN106148204A CN106148204A CN201610706345.8A CN201610706345A CN106148204A CN 106148204 A CN106148204 A CN 106148204A CN 201610706345 A CN201610706345 A CN 201610706345A CN 106148204 A CN106148204 A CN 106148204A
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- fructus jujubae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention belongs to edible fungus fermented culture technique field, refer specifically to a kind of Ganoderma liquid fermentation medium and preparation method thereof.The described medium pH of the present invention is 6.0 6.5, containing Rhizoma Solani tuber osi, Semen Maydis powder, glucose, KH in described culture medium2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution, distilled water；The preparation method of Fructus Jujubae slag extracting solution is: be dried to constant weight by Fructus Jujubae slag, adds composite enzyme solution, after reaction, regulates pH value of solution with sulfuric acid solution, filters, obtain Fructus Jujubae slag extracting solution after reacting by heating；Rhizoma Solani tuber osi after Semen Maydis powder and stripping and slicing is added in distilled water, boils, filter, filtrate adds Semen Maydis powder, glucose, KH2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution stir, and sterilizing obtains Ganoderma liquid fermentation medium.Grifola frondosa liquid fermentation use this culture medium to have transformation efficiency is high, cultivation cycle is short, be difficult to the features such as bacteria infection, and Grifolas frondosa germ pompon yield is improved.
Description
Technical field
The invention belongs to edible fungus fermented culture technique field, refer specifically to a kind of Ganoderma liquid fermentation medium and preparation thereof
Method.
Technical background
Ganoderma also referred to as " China Ganoderma ", sweet in the mouth, property is put down.Main containing ergosterol, organic acid, glucosamine, polysaccharide,
Resin, mannitol, polysaccharide polyol, fatty acid, again containing alkaloid, lactone, coumarin, water soluble protein and multiple enzyme.Ganoderma
Range of application widely, no matter the heart, lung, liver,spleen,kidney deficiency of five ZANG-organs are weak, all can take.Ganoderma is cured the disease to plant and is related to breathing, following
Each systems such as ring, digestion, nerve, endocrine and immunity；Contain inside and outside, woman, youngster, face each section disease, its basic reason,
It is that Ganoderma strengthening the body resistance, strengthens immunologic function, improve the great function of Abwehrkraft des Koepers.It is different from general medicine to certain
Kind of disease and play therapeutical effect, be also different from the general nutritional health food deficiency only to nutrient in a certain respect carry out supplementing and
Strengthening, but two-ways regulation function of human body balance on the whole, transfer internal body vigor, regulate human metabolism's function,
Improve autoimmune ability, promote whole internal organs or organ function normalization.
At present, correlational study both domestic and external is concentrated mainly on the extraction of Ganoderma functional factor and pharmacology and nutritive effect.
But, in the artificial culture field of Ganoderma sporophore, but limited by technology and environment, it is difficult to carry out industrialized production and
Civil nature cultivation uses widely, and meanwhile, this technical field is also difficult to commercially use.Therefore, people are to Ganoderma health-care effect
The shortage with Ganoderma sporophore recoverable amount of craving for therapeutic efficiency creates significant contradiction.In recent years, profit due to liquid deep
Layer cultivates Ganoderma not by season and raw-material to be affected, and the strain cultivation cycle is short, cell age neat, low cost, can quickly increase
Grow somatic cells, significantly improve the advantages such as production efficiency, get more and more people's extensive concerning, there is good application prospect, as
What effectively uses fermenting organism technology that Ganoderma carries out deep drainpipe, and improves the yield of Ganoderma active component further to exploitation
Ganoderma product tool significance.
Shi Chan Fructus Jujubae big country of China, in recent years, Fructus Jujubae has been carried out in depth studying by people, develops many Fructus Jujubae products, as
Jujube wine, Fructus Jujubae juice etc., but along with the industrial development of Fructus Jujubae, the most inevitably bring many commercial production waste materials, such as Fructus Jujubae
Slag, Fructus Jujubae slag typically contains certain nutrient substance, easily corrupt, and environment can cause serious pollution, past Fructus Jujubae slag waste material one
As be as animal feed or Fertilizer application, cause the waste of resource, and process not in time and also can cause environmental pollution.
Therefore, the comprehensive utilization technique of exploitation Fructus Jujubae slag, Fructus Jujubae slag is turned waste into wealth, good economic benefit can be brought, can close again
Reason utilizes resource, alleviates environmental pollution burden.
Summary of the invention
It is an object of the invention to provide a kind of culture medium that disclosure satisfy that Ganoderma Fermentative growth needs, this culture medium can carry
The increment of high Ganoderma mycelia, has the features such as transformation efficiency is high, cultivation cycle is short.
It is a further object of the present invention to provide the preparation method of a kind of Ganoderma fluid medium, the method is simple to operate, suitable
Close industrialized production.
A kind of Ganoderma liquid fermentation medium, pH is 6.0-6.5；Every liter of described culture medium contains: Rhizoma Solani tuber osi 180-200g,
Semen Maydis powder 1.2-1.4g, glucose 18-20g, KH2PO41.2-1.5g、MgSO4·7H2O 0.7-0.8g, Fructus Jujubae slag extracting solution 45-
60mL, surplus is distilled water；
The preparation method employing following steps of described Fructus Jujubae slag extracting solution:
(1) Fructus Jujubae slag is placed in baking oven 60-65 DEG C be dried to constant weight, weighs 10-15g and be put in reactor；
(2) in a kettle. add 500mL concentration be 0.8-1.0mg/L, pH be the composite enzyme solution of 5.5, after mix homogeneously
React 2-3 hour under the conditions of 55-60 DEG C；
(3) pH value of solution in sulfuric acid solution regulating step (2) reactor of 1.0-6.0wt% is used to 2.0-4.0, to be added by reactor
Heat, to 95-100 DEG C, reacts 60-80min, after reaction terminates, filters, obtains filtrate, adds distilled water to 500mL, obtains Fructus Jujubae slag and extracts
Liquid；
The preparation method of described culture medium is: is added in 800mL distilled water by the Rhizoma Solani tuber osi after Semen Maydis powder and stripping and slicing, boils 20-
30min, filters, obtains filtrate, adds Semen Maydis powder, glucose, KH in filtrate2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution stir
Uniformly, adding distilled water to 1000mL, regulation pH value of solution is 6-6.5, sterilizing 20-40min under the conditions of 121 DEG C, cooling, obtains Ganoderma liquid
Body fermentation medium.
Preferably, Ganoderma liquid fermentation culture medium, pH is 6.2；Every liter of described culture medium contains: Rhizoma Solani tuber osi 190g, jade
Rice flour 1.3g, glucose 20g, KH2PO41.4g、MgSO4·7H2O 0.7g, Fructus Jujubae slag extracting solution 50mL, surplus are distilled water.
Preferably, the enzyme of the following mass fraction of compound enzyme described in step (2) is compounding forms: pectase 0-10%, cellulose
Enzyme 85-95%, serrapeptass 1-2%, papain 1-4%.
Recent years, Fructus Jujubae has been carried out in depth studying by people, develops many Fructus Jujubae products, such as jujube wine, Fructus Jujubae juice etc., but
Along with the industrial development of Fructus Jujubae, the most inevitably bring many commercial production waste materials, such as Fructus Jujubae slag.Research finds Fructus Jujubae
Industrial waste in containing the nutrient substance such as a lot of polysaccharide, triterpenic acid, vitamin, the jujube extract of the present invention uses exactly
The Fructus Jujubae slag stated is prepared from, and is that Fructus Jujubae slag is carried out secondary development, therefrom effective component extracting, to reach to improve utilization of resources
Rate, drop oligosaprobic purpose, bring good Social benefit and economic benefit simultaneously.
Ganoderma strain of the present invention can use any active strain can being commercially available on market.
Beneficial effect
The present invention adds Fructus Jujubae slag extracting solution in Ganoderma fluid medium, makes culture medium disclosure satisfy that the need of Ganoderma liquid fermentation
Asking, the raw material of this culture medium is simple, be easy to get, with low cost.Utilize this culture medium produce G.japonicum mycelium have fast growth,
Cycle is short, transformation efficiency is high, technique is simple, low cost and other advantages, and the industrial fermentation being suitable to Ganoderma produces, and utilizes side of the present invention
Method cultivates the G.japonicum mycelium of gained can meet the market demand larger.The culture medium preparation method operation letter that the present invention provides
Single, it is suitable for industrialized production.
Detailed description of the invention
Below by embodiment, the present invention will be further elaborated, it should explanation, the description below merely to
Explain the present invention, its content is not defined.
The present invention uses 6M sodium hydroxide solution or the pH of hydrochloric acid regulation Ganoderma liquid fermentation medium.
Embodiment 1
Ganoderma liquid fermentation medium, pH is 6.0；Every liter of described culture medium contains: 180 grams of Rhizoma Solani tuber osi, Semen Maydis powder 1.4g, Fructus Vitis viniferae
Sugar 20g, KH2PO41.5g、MgSO4·7H2O 0.7g, Fructus Jujubae slag extracting solution 60mL, surplus is distilled water；
Wherein the preparation method of Fructus Jujubae slag extracting solution is:
(1) Fructus Jujubae slag is placed in baking oven it is dried to constant weight in 60-65 DEG C, weigh 10g and be put in reactor；
(2) in a kettle. add 500mL concentration be 0.8mg/L, pH be the composite enzyme solution of 5.5, at 55 DEG C after mix homogeneously
Under the conditions of react 3 hours；Wherein compound enzyme is by 0.38mg cellulase, 0.008mg serrapeptass and 0.012mg papain
Compound and form；
(3) pH value of solution in sulfuric acid solution regulating step (2) reactor of 1.0wt% is used to 2.0, reactor to be heated to 95
DEG C, react 80min, after reaction terminates, filter, obtain filtrate, add distilled water to 500mL, obtain Fructus Jujubae slag extracting solution；
The preparation method of Ganoderma fluid medium:
Rhizoma Solani tuber osi after Semen Maydis powder and stripping and slicing is added in 800mL distilled water, boils 20min, three layers of filtered through gauze, obtain filtrate, in filter
Liquid adds glucose, KH2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution, boil, and makes addition raw material be completely dissolved, adds distillation
Water is to 1000mL, and regulation pH is sterilizing 20min under the conditions of 6.0,121 DEG C, and cooling obtains Ganoderma liquid fermentation medium.
Embodiment 2
Ganoderma liquid fermentation medium, pH is 6.5；Every liter of described culture medium contains: 200 grams of Rhizoma Solani tuber osi, Semen Maydis powder 1.2g, Fructus Vitis viniferae
Sugar 18g, KH2PO41.2g、MgSO4·7H2O 0.8g, Fructus Jujubae slag extracting solution 45mL, surplus is distilled water；
Wherein the preparation method of Fructus Jujubae slag extracting solution is:
(1) Fructus Jujubae slag is placed in baking oven it is dried to constant weight in 60-65 DEG C, weigh 15g and be put in reactor；
(2) in a kettle. add 500mL concentration be 1.0mg/L, pH be the composite enzyme solution of 5.5, at 60 DEG C after mix homogeneously
Under the conditions of react 3 hours；Wherein compound enzyme by 0.05mg pectase, 0.425mg cellulase, 0.005mg serrapeptass and
0.02mg papain is compounding to be formed；
(3) pH value of solution in sulfuric acid solution regulating step (2) reactor of 6.0wt% is used to 4.0, reactor to be heated to 100
DEG C, react 60min, after reaction terminates, filter, obtain filtrate, add distilled water to 500mL, obtain Fructus Jujubae slag extracting solution；
The preparation method of Ganoderma fluid medium:
Being added in 800mL distilled water by Rhizoma Solani tuber osi after Semen Maydis powder and stripping and slicing, boil 30min, three layers of filtered through gauze filter, and obtain filter
Liquid, adds glucose, KH in filtrate2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution, boil, and makes addition raw material be completely dissolved,
Adding distilled water to 1000mL, regulation pH is sterilizing 20-40min under the conditions of 6.5,121 DEG C, and cooling obtains Ganoderma liquid fermentation and culture
Base.
Embodiment 3
Ganoderma liquid fermentation medium, pH is 6.2；Every liter of described culture medium contains: 190 grams of Rhizoma Solani tuber osi, Semen Maydis powder 1.3g, Fructus Vitis viniferae
Sugar 20g, KH2PO41.4g、MgSO4·7H2O 0.7g, Fructus Jujubae slag extracting solution 50mL, surplus is distilled water；
Wherein the preparation method of Fructus Jujubae slag extracting solution is:
(1) Fructus Jujubae slag is placed in baking oven 60-65 DEG C be dried to constant weight, weighs 12g and be put in reactor；
(2) in a kettle. add 500mL concentration be 1.0mg/L, pH be the composite enzyme solution of 5.5, at 60 DEG C after mix homogeneously
Under the conditions of react 2-3 hour；Wherein compound enzyme by 0.04mg pectase, 0.45mg cellulase, 0.005mg serrapeptass and
0.005mg papain is compounding to be formed；
(3) pH value of solution in sulfuric acid solution regulating step (2) reactor of 4.0wt% is used to 3.0, reactor to be heated to 98
DEG C, react 70min, after reaction terminates, filter, obtain filtrate, add distilled water to 500mL, obtain Fructus Jujubae slag extracting solution；
The preparation method of Ganoderma fluid medium:
Rhizoma Solani tuber osi after Semen Maydis powder and stripping and slicing is added in 800mL distilled water, boils 30min, filter, obtain filtrate, add in filtrate
Glucose, KH2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution, boil, and makes addition raw material be completely dissolved, adds distilled water extremely
1000mL, regulation pH is sterilizing 30min under the conditions of 6.2,121 DEG C, and cooling obtains Ganoderma liquid fermentation medium.
Embodiment 4
Ganoderma liquid fermentation medium, pH is 6.5；Every liter of described culture medium contains: 190 grams of Rhizoma Solani tuber osi, Semen Maydis powder 1.3g, Fructus Vitis viniferae
Sugar 20g, KH2PO41.4g、MgSO4·7H2O 0.7g, Fructus Jujubae slag extracting solution 50mL, surplus is distilled water；
The preparation method of Fructus Jujubae slag extracting solution is:
(1) Fructus Jujubae slag is placed in baking oven 60-65 DEG C be dried to constant weight, weighs 12g and be put in reactor；
(2) in a kettle. add 500mL concentration be 1.0mg/L, pH be the composite enzyme solution of 5.5, at 60 DEG C after mix homogeneously
Under the conditions of react 3 hours；Wherein compound enzyme by 0.04mg hemicellulase, 0.45mg cellulase, 0.005mg serrapeptass and
0.005mg papain is compounding to be formed；
(3) pH value of solution in sulfuric acid solution regulating step (2) reactor of 4.0wt% is used to 3.0, reactor to be heated to 98
DEG C, react 70min, after reaction terminates, filter, obtain filtrate, add distilled water to 500mL, obtain Fructus Jujubae slag extracting solution；
The preparation method of Ganoderma fluid medium is:
Semen Maydis powder and stripping and slicing Rhizoma Solani tuber osi are added in 800mL distilled water, boils 30min, filter, obtain filtrate, filtrate adds Portugal
Grape sugar, KH2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution stir, and add distilled water to 1000mL, and regulation pH is 6.5,121
Sterilizing 30min under the conditions of DEG C, cooling, obtain Ganoderma liquid fermentation medium.
Comparative example
Ganoderma liquid fermentation medium, pH is 6.5；Described culture medium is made up of following material: 190 grams of Rhizoma Solani tuber osi, Semen Maydis powder 1.3g,
Glucose 20g, KH2PO41.4g、MgSO4·7H2O 0.7g, add distilled water to 1000mL；
The preparation method of Ganoderma fluid medium is:
Semen Maydis powder 1.3g and 190g stripping and slicing Rhizoma Solani tuber osi are added in 800mL distilled water, boils 30min, filter, obtain filtrate, filtrate
Middle addition, glucose 20g, KH2PO41.4g、MgSO4·7H2O 0.7g stirs, and boils, and makes addition raw material be completely dissolved,
Adding distilled water to 1000mL, regulation pH is sterilizing 30min under the conditions of 6.5,121 DEG C, and cooling obtains Ganoderma liquid fermentation medium.
Liquid fermentation medium above-described embodiment 1-4 and comparative example prepared carries out Ganoderma fermentation:
According to 5v/v% inoculum concentration equipped with 100mL fluid medium 750mL triangle shaking flask inoculate Ganoderma strain, temperature 26 DEG C,
Rotating speed 120r/min, constant-temperature table is cultivated 8d, is obtained Ganoderma mycelium pellet, collects Ganoderma mycelium pellet by 8 layers of filtered through gauze, goes as far as possible
Except wherein culture fluid, loading freezing collecting pipe ,-18 DEG C of freezing rearmounted-70 DEG C of freezings that freeze are drained, and obtain Ganoderma mycelia dry powder, and-18
DEG C airtight preservation.Above-described embodiment and comparative example respectively cultivate three shaking flasks, and the G.japonicum mycelium dry mass that liquid fermentation obtains is
Three shaking flasks meansigma methods, its data are shown in Table 1:
Table 1
Claims (3)
1. a Ganoderma liquid fermentation medium, it is characterised in that pH is 6.0-6.5；Every liter of described culture medium contains: Rhizoma Solani tuber osi
180-200g, Semen Maydis powder 1.2-1.4g, glucose 18-20g, KH2PO41.2-1.5g、MgSO4·7H2O 0.7-0.8g, Fructus Jujubae slag
Extracting solution 45-60mL, surplus is distilled water；
The preparation method employing following steps of described Fructus Jujubae slag extracting solution:
(1) Fructus Jujubae slag is placed in baking oven 60-65 DEG C be dried to constant weight, weighs 10-15g and be put in reactor；
(2) in a kettle. add 500mL concentration be 0.8-1.0mg/L, pH be the composite enzyme solution of 5.5, after mix homogeneously
React 2-3 hour under the conditions of 55-60 DEG C；
(3) pH value of solution in sulfuric acid solution regulating step (2) reactor of 1.0-6.0wt% is used to 2.0-4.0, to be added by reactor
Heat, to 95-100 DEG C, reacts 60-80min, after reaction terminates, filters, obtains filtrate, adds distilled water to 500mL, obtains Fructus Jujubae slag and extracts
Liquid；
The preparation method of described culture medium is: is added in 800mL distilled water by the Rhizoma Solani tuber osi after Semen Maydis powder and stripping and slicing, boils 20-
30min, filters, obtains filtrate, adds Semen Maydis powder, glucose, KH in filtrate2PO4、MgSO4·7H2O, Fructus Jujubae slag extracting solution stir
Uniformly, adding distilled water to 1000mL, regulation pH value of solution is 6-6.5, sterilizing 20-40min under the conditions of 121 DEG C, cooling, obtains Ganoderma liquid
Body fermentation medium.
Ganoderma liquid fermentation culture medium the most according to claim 1, it is characterised in that pH is 6.2；Every liter of described cultivation
Base contains: Rhizoma Solani tuber osi 190g, Semen Maydis powder 1.3g, glucose 20g, KH2PO41.4g、MgSO4·7H2O 0.7g, Fructus Jujubae slag extract
Liquid 50mL, surplus are distilled water.
Ganoderma liquid fermentation culture medium the most according to claim 1, it is characterised in that the compound enzyme described in step (2)
The enzyme of following mass fraction is compounding to be formed: pectase 0-10%, cellulase 85-95%, serrapeptass 1-2%, papain 1-
4%。
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| CN108934769A (en) * | 2018-06-26 | 2018-12-07 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of Amauroderma ruda (Berk) Pat submerged fermentation culture medium and preparation method thereof |
| CN109370920A (en) * | 2018-12-11 | 2019-02-22 | 迁安贝丽莱生物科技有限公司 | A kind of cultural method and a kind of purple sesame liquid fermentation method of purple sesame bacterial strain |
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