CN105753531A - Cultivation medium for liquid strains of black fungi and method for preparing cultivation medium - Google Patents
Cultivation medium for liquid strains of black fungi and method for preparing cultivation medium Download PDFInfo
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- CN105753531A CN105753531A CN201511011255.9A CN201511011255A CN105753531A CN 105753531 A CN105753531 A CN 105753531A CN 201511011255 A CN201511011255 A CN 201511011255A CN 105753531 A CN105753531 A CN 105753531A
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- cultivation medium
- culture medium
- waste liquid
- liquid
- auricularia
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a cultivation medium for liquid strains of black fungi and a method for preparing the cultivation medium.The cultivation medium and the method have the advantages that the cultivation medium contains few components and is easy to obtain and low in cost, waste liquid of distillers' grains generated in procedures for producing alcohol by the aid of fermentation processes is utilized, to be more specific, the waste liquid of the corn distillers' grains is an important composition portion of the cultivation medium and contains 92-95% of water, remaining 5-8% of dry substances in the waste liquid contain abundant nutrition ingredients such as carbohydrate, proteins, celluloses, pentosan, caramel, fat, abundant vitamins B and auxin, and accordingly, nutrition ingredients necessary for growth of the liquid strains of the black fungi can be provided by the cultivation medium; the method for preparing the cultivation medium is relatively simple and includes controllable, simple and convenient operation steps, the problem of pollution due to the waste liquid drained from an alcohol plant can be solved if the cultivation medium is used for cultivating the liquid strains of the black fungi, and the cost can be reduced to a great extent; mycelia of the black fungi can grow fast and are high in activity and content, a formula for the cultivation medium for the liquid strain is favorable for standard production, and the cultivation medium and the method have obvious economic and social benefits.
Description
Technical field
The present invention relates to bioengineering field, particularly a kind of Auricularia liquid spawn culture medium and preparation method thereof.
Background technology
Auricularia is under the jurisdiction of Basidiomycetes, Auriculariale, Auriculariaceae, Auricularia, is commonly called as Auricularia, brightness program, the ears or side handles of a utensil, room ear, cloud ear, black dish etc., and Auricularia meat is fine and smooth, and crisp smooth mouth is nutritious.The nutritional labeling of Auricularia, according to modern science analysis, containing 10.6 grams of protein in every 100 grams of dry products, 0.2 gram of fat, carbohydrate 65 grams, crude fibre 7 grams, calcium 375 milligrams, 201 milligrams of phosphorus, ferrum 185 milligrams, additionally contain vitaminB10 .15 milligram, vitamin B2 0.55 milligram, 2.7 milligrams of nicotinic acid.Auricularia not only has significantly high nutritive value, also has higher medical value.
Fermentative Production ethanol is the main method that various countries produce that ethanol adopts, and fermentation liquid, after steaming ethanol, just becomes a kind of waste material and left behind, and this waste material is called drunk fish.Often produce 1 ton of ethanol and to discharge 10 ~ 15 tons of drunk fish, the BOD value of cereal materials drunk fish, commonly reach 20000 ~ 30000mg/L, COD value is up to 40000 ~ 50000mg/L, if directly environment can be caused serious pollution by discharge.
It is formulated that existing Auricularia liquid spawn culture medium mainly selects Semen Maydis powder, Rhizoma Solani tuber osi, wheat bran, peptone, yeast extract, white sugar and inorganic salt to join etc., there is raw material composition many, the problems such as Costco Wholesale is high.In preparation process, it is necessary to carry out high cooking, there is the problems such as material dissolution is insufficient, particulate matter is many, sterilization time length, preparation process are complicated.There is the shortcomings such as slow, the cultivation cycle length of mycelial growth rate in the liquid spawn prepared, it is impossible to is standardized and factorial praluction.
Summary of the invention
The present invention is to solve the above-mentioned deficiency existing for prior art, it is proposed to a kind of raw material composition is few, Auricularia liquid spawn culture medium with low cost, and the preparation method of this kind of culture medium.
The technical solution of the present invention is: a kind of Auricularia liquid spawn culture medium, it is characterised in that: described culture medium is made up of raw material and water, and wherein said raw material includes the component of following mass percent concentration:
Maize alcohol stillage waste liquid 2-4%
Sucrose 1-3%
Potassium dihydrogen phosphate 0.05-0.15%
Magnesium sulfate 0.025-0.075%
Water surplus.
Described culture medium is made up of raw material and water, and wherein said raw material includes the component of following mass percent concentration:
Maize alcohol stillage waste liquid 3%
Sucrose 2%
Potassium dihydrogen phosphate 0.1%
Magnesium sulfate 0.05%
Water surplus.
A kind of preparation method of Auricularia liquid spawn culture medium as above, it is characterised in that: described method carries out according to following steps:
First the component of following mass percent concentration is weighed: the maize alcohol stillage waste liquid of 2-4%, the sucrose of 1-3%, the potassium dihydrogen phosphate of 0.05-0.15% and the magnesium sulfate of 0.025-0.075%, then first part of hot water of 30 times of volumes of maize alcohol stillage waste liquid addition 70-80 DEG C will stir and make it dissolve, boil and remove oils and fats contained therein, obtain the first aqueous solution
Second part of hot water of 30 times of volumes of sucrose, potassium dihydrogen phosphate and magnesium sulfate addition 70-80 DEG C will stir and make it dissolve, it is thus achieved that the second aqueous solution,
By the first aqueous solution and the second aqueous solution Homogeneous phase mixing, it is thus achieved that mixed solution,
The pH value adjusting mixed solution with sodium hydroxide is 6.0,
Being placed in fermentation tank and carry out sterilizing by mixed solution after adjusting pH value, sterilising temp is 121 DEG C, and sterilization time is 60min, and namely sterilizing obtains Auricularia liquid spawn culture medium after completing.
The present invention compared with the existing technology, has the advantage that
Auricularia liquid spawn culture medium disclosed in this invention, its composition component is few, obtain easily, with low cost, it utilizes the drunk fish produced in fermentative Production ethanol process, specifically utilizes maize alcohol stillage waste liquid as the important component part of culture medium, due in maize alcohol stillage waste liquid, moisture 92 ~ 95%, containing the nutrition that carbohydrate, protein and cellulose, poly-pentose, caramel, fat, abundant vitamin B group and auxin etc. are abundant in all the other dries of 5 ~ 8%, it is provided that nutrition necessary to Auricularia liquid spawn length.And the method for preparing this culture medium is also relatively easy, operating procedure is controlled, easy, this culture medium is utilized to carry out the cultivation of Auricularia liquid spawn, both the pollution of Alcohol Plant's discharge waste liquid had been solved, cost is made again to be greatly lowered, agaric mycelium growth simultaneously is fast, energetic, content is high, and this liquid spawn culture medium formula is conducive to standardized production, has significant economic benefit and social benefit.
Detailed description of the invention
The specific embodiment of the present invention is described below.
Embodiment one
The preparation method of a kind of Auricularia liquid spawn culture medium, carries out according to following steps:
First the component of following mass percent concentration is weighed: the maize alcohol stillage waste liquid of 2%, the sucrose of 1%, the potassium dihydrogen phosphate of 0.05% and the magnesium sulfate of 0.025%, then maize alcohol stillage waste liquid is added and the 15L hot water of 70-80 DEG C stirs and makes it dissolve, boil and remove oils and fats contained therein, obtain the first aqueous solution
Sucrose, potassium dihydrogen phosphate and magnesium sulfate are added and the 15L hot water of 70-80 DEG C stir and makes it dissolve, it is thus achieved that the second aqueous solution,
By the first aqueous solution and the second aqueous solution Homogeneous phase mixing, it is thus achieved that mixed solution,
The pH value adjusting mixed solution with sodium hydroxide is 6.0,
Being placed in the fermentation tank of 50L and carry out sterilizing by mixed solution after adjusting pH value, sterilising temp is 121 DEG C, and sterilization time is 60min, utilizes all the other moisture content of condensed water polishing produced in sterilization process, and namely sterilizing obtains Auricularia liquid spawn culture medium after completing.
Inoculated and cultured: Auricularia liquid spawn culture medium is cooled to less than 26 DEG C in fermentation tank, in the ratio of 5%, accesses Auricularia liquid spawn, regulate cultivation temperature 23 ~ 25 DEG C, fermentation tank passes into filtrated air and carries out aerobic cultivation, fermentation culture 6 days, can be prepared by Auricularia liquid spawn.
Embodiment two
The preparation method of a kind of Auricularia liquid spawn culture medium, carries out according to following steps:
First the component of following mass percent concentration is weighed: the maize alcohol stillage waste liquid of 3%, the sucrose of 2%, the potassium dihydrogen phosphate of 0.1% and the magnesium sulfate of 0.05%, then maize alcohol stillage waste liquid is added and the 15L hot water of 70-80 DEG C stirs and makes it dissolve, boil and remove oils and fats contained therein, obtain the first aqueous solution
Sucrose, potassium dihydrogen phosphate and magnesium sulfate are added and the 15L hot water of 70-80 DEG C stir and makes it dissolve, it is thus achieved that the second aqueous solution,
By the first aqueous solution and the second aqueous solution Homogeneous phase mixing, it is thus achieved that mixed solution,
The pH value adjusting mixed solution with sodium hydroxide is 6.0,
Being placed in the fermentation tank of 50L and carry out sterilizing by mixed solution after adjusting pH value, sterilising temp is 121 DEG C, and sterilization time is 60min, utilizes all the other moisture content of condensed water polishing produced in sterilization process, and namely sterilizing obtains Auricularia liquid spawn culture medium after completing.
Inoculated and cultured: Auricularia liquid spawn culture medium is cooled to less than 26 DEG C in fermentation tank, in the ratio of 5%, accesses Auricularia liquid spawn, regulate cultivation temperature 23 ~ 25 DEG C, fermentation tank passes into filtrated air and carries out aerobic cultivation, fermentation culture 4 days, can be prepared by Auricularia liquid spawn.
Embodiment three
The preparation method of a kind of Auricularia liquid spawn culture medium, carries out according to following steps:
First the component of following mass percent concentration is weighed: the maize alcohol stillage waste liquid of 4%, the sucrose of 3%, the potassium dihydrogen phosphate of 0.15% and the magnesium sulfate of 0.075%, then maize alcohol stillage waste liquid is added and the 15L hot water of 70-80 DEG C stirs and makes it dissolve, boil and remove oils and fats contained therein, obtain the first aqueous solution
Sucrose, potassium dihydrogen phosphate and magnesium sulfate are added and the 15L hot water of 70-80 DEG C stir and makes it dissolve, it is thus achieved that the second aqueous solution,
By the first aqueous solution and the second aqueous solution Homogeneous phase mixing, it is thus achieved that mixed solution,
The pH value adjusting mixed solution with sodium hydroxide is 6.0,
Being placed in the fermentation tank of 50L and carry out sterilizing by mixed solution after adjusting pH value, sterilising temp is 121 DEG C, and sterilization time is 60min, utilizes all the other moisture content of condensed water polishing produced in sterilization process, and namely sterilizing obtains Auricularia liquid spawn culture medium after completing.
Inoculated and cultured: Auricularia liquid spawn culture medium is cooled to less than 26 DEG C in fermentation tank, in the ratio of 5%, accesses Auricularia liquid spawn, regulate cultivation temperature 23 ~ 25 DEG C, fermentation tank passes into filtrated air and carries out aerobic cultivation, fermentation culture 5 days, can be prepared by Auricularia liquid spawn.
Claims (3)
1. an Auricularia liquid spawn culture medium, it is characterised in that: described culture medium is made up of raw material and water, and wherein said raw material includes the component of following mass percent concentration:
Maize alcohol stillage waste liquid 2-4%
Sucrose 1-3%
Potassium dihydrogen phosphate 0.05-0.15%
Magnesium sulfate 0.025-0.075%
Water surplus.
2. Auricularia liquid spawn culture medium according to claim 1, it is characterised in that: described culture medium is made up of raw material and water, and wherein said raw material includes the component of following mass percent concentration:
Maize alcohol stillage waste liquid 3%
Sucrose 2%
Potassium dihydrogen phosphate 0.1%
Magnesium sulfate 0.05%
Water surplus.
3. the preparation method of an Auricularia liquid spawn culture medium as claimed in claim 1, it is characterised in that: described method carries out according to following steps:
First the component of following mass percent concentration is weighed: the maize alcohol stillage waste liquid of 2-4%, the sucrose of 1-3%, the potassium dihydrogen phosphate of 0.05-0.15% and the magnesium sulfate of 0.025-0.075%, then first part of hot water of 30 times of volumes of maize alcohol stillage waste liquid addition 70-80 DEG C will stir and make it dissolve, boil and remove oils and fats contained therein, obtain the first aqueous solution
Second part of hot water of 30 times of volumes of sucrose, potassium dihydrogen phosphate and magnesium sulfate addition 70-80 DEG C will stir and make it dissolve, it is thus achieved that the second aqueous solution,
By the first aqueous solution and the second aqueous solution Homogeneous phase mixing, it is thus achieved that mixed solution,
The pH value adjusting mixed solution with sodium hydroxide is 6.0,
Being placed in fermentation tank and carry out sterilizing by mixed solution after adjusting pH value, sterilising temp is 121 DEG C, and sterilization time is 60min, and namely sterilizing obtains Auricularia liquid spawn culture medium after completing.
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| CN201511011255.9A CN105753531A (en) | 2015-12-30 | 2015-12-30 | Cultivation medium for liquid strains of black fungi and method for preparing cultivation medium |
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| CN201511011255.9A CN105753531A (en) | 2015-12-30 | 2015-12-30 | Cultivation medium for liquid strains of black fungi and method for preparing cultivation medium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106396847A (en) * | 2016-08-26 | 2017-02-15 | 福建省元盛农业综合开发有限公司 | Black fungus culture medium taking lotus seedpod and lotus seed shell as raw materials |
| CN106434364A (en) * | 2016-09-19 | 2017-02-22 | 于汇 | Production, preservation and application technology for liquid subpackaged strain |
| CN108546174A (en) * | 2018-07-11 | 2018-09-18 | 广东泓睿科技有限公司 | A kind of selenium-enriched edible mushroom culture medium and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104261963A (en) * | 2014-08-28 | 2015-01-07 | 岳西县利泰农业发展有限责任公司 | Vinasse-containing black fungus culture medium and preparation method thereof |
| CN104876691A (en) * | 2015-05-11 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Compost for black fungus cultivation and preparation method of compost |
-
2015
- 2015-12-30 CN CN201511011255.9A patent/CN105753531A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104261963A (en) * | 2014-08-28 | 2015-01-07 | 岳西县利泰农业发展有限责任公司 | Vinasse-containing black fungus culture medium and preparation method thereof |
| CN104876691A (en) * | 2015-05-11 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Compost for black fungus cultivation and preparation method of compost |
Non-Patent Citations (3)
| Title |
|---|
| 张远琼: "利用酒糟生产食用菌的初步研究", 《四川师范大学学报（自然科学版）》 * |
| 矫天育: "黑木耳液体摇瓶培养基营养筛选", 《食用菌》 * |
| 荆瑞勇 等: "黑木耳液体培养基筛选及发酵条件优化", 《食用菌》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106396847A (en) * | 2016-08-26 | 2017-02-15 | 福建省元盛农业综合开发有限公司 | Black fungus culture medium taking lotus seedpod and lotus seed shell as raw materials |
| CN106434364A (en) * | 2016-09-19 | 2017-02-22 | 于汇 | Production, preservation and application technology for liquid subpackaged strain |
| CN108546174A (en) * | 2018-07-11 | 2018-09-18 | 广东泓睿科技有限公司 | A kind of selenium-enriched edible mushroom culture medium and preparation method thereof |
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Application publication date: 20160713 |
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