CN105110957A - High-bioactivity efficient culture medium for stropharia rugoso-annulata and preparing method of culture medium - Google Patents
High-bioactivity efficient culture medium for stropharia rugoso-annulata and preparing method of culture medium Download PDFInfo
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- CN105110957A CN105110957A CN201510572943.6A CN201510572943A CN105110957A CN 105110957 A CN105110957 A CN 105110957A CN 201510572943 A CN201510572943 A CN 201510572943A CN 105110957 A CN105110957 A CN 105110957A
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- culture medium
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- annulata
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- stropharia rugoso
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a high-bioactivity efficient culture medium for stropharia rugoso-annulata. The culture medium is prepared from glucose syrup, ganoderma lucidum polysaccharides, sodium lactate, wormcast, baking powder, cedar wood chips, bean dregs, cassava dregs, sterilized biogas slurry, coarse whiting, L-sodium glutamate, vegetation grass root residues, quartz sand, decoction dregs, bacillus thuringiensis, cryytococcus neoformans, spirulina dregs, fermentation microflora, zinc gluconate, chitosan, acidic amino acid, coccidioides immites powder, loofah sponge and loofah sponge fibers by weight. As the cedar wood chips are matched with other organic and inorganic substances to prepare the culture medium for stropharia rugoso-annulata, the culture medium is rich in nutrition; a culture medium porous conditioner is prepared from saccharomycetes composite loaded by the loofah sponge and the zinc gluconate, the chitosan, the acidic amino acid which are located by the loofah sponge and used for adjusting adsorption and distribution of nutritional ingredients and nutritional elements of the culture medium and achieving the water retention and water repellent effects of the culture medium, and a good environment and nutritional substances are provided for growth of stropharia rugoso-annulata.
Description
Technical field
The present invention relates to culture medium of edible fungus technical field, particularly relate to a kind of Stropharia rugoso-annulata high biological activity efficient culture medium and preparation method thereof.
Background technology
Stropharia rugoso-annulata (Strophariarugoso-annulate), also known as the red Stropharia rugoso-annulata of wine, Stropharia rugoso annulata, belongs to Agaricales, Strophariaceae, Stropharia.Since nineteen twenty-two American finds, extensively cultivate in European and American countries.Stropharia rugoso-annulata is nutritious, and meat is tender delicious, containing multiple human body essential amino acid and VITAMIN, and the pharmaceutical use of the various diseases such as tool prevention coronary heart disease, very popular, be that the edible mushrooms one of cultivation is recommended to developing country by Food and Argriculture OrganizationFAO.
Vegetable Sponge of Luffa is the vascular bundle that the maturation of cucurbitaceous plant sponge gourd or Guangdong sponge gourd is crossed, it is a kind of light natural macromolecular material with successive type hyperchannel porous network structure, there is pore texture prosperity, stable in physicochemical property, good biocompatibility, can as enzyme or zymogenic carrier.Meanwhile, Vegetable Sponge of Luffa has large specific surface area, has good adsorptivity and carrier effect, may be used for realizing the absorption of different nutriments and loading and then plays effects such as controlling its distribution, its release rate.
Summary of the invention
The present invention is directed to the cultivation demand of Stropharia rugoso-annulata, a kind of Stropharia rugoso-annulata high biological activity efficient culture medium and preparation method thereof is provided.
The present invention is achieved by the following technical solutions:
A kind of Stropharia rugoso-annulata high biological activity efficient culture medium, comprise following parts by weight of component: glucose syrup 3-4, ganoderan 3-4, Sodium.alpha.-hydroxypropionate 2-3, wormcast 12-14, baking powder 1.4-1.6, Chinese fir wood chip 60-70, bean dregs 30-32, manioc waste 15-17, sterilizing natural pond liquid 15-20, coarse whiting 1.3-1.6, L-sodium 0.9-1.1, vegetation grass roots slag 20-30, quartz sand 15-17, dregs of a decoction 6-7, bacillus thuringiensis 0.7-0.8, geotrichum candidum 0.9-1, spiral algae-residue 16-18, fermentative microflora 1.6-1.9, zinc gluconate 1-1.4, chitosan 2-3, acidic amino acid 2.4-2.6, steamed stuffed bun bacterium powder 2-3, Vegetable Sponge of Luffa 15-17, loofah fiber 5-6, appropriate water.
A kind of Stropharia rugoso-annulata high biological activity efficient culture medium preparation method, comprises the following steps:
(1) Vegetable Sponge of Luffa is put into pulverizer and pulverize 2-3min, obtain Loofah powder to mince, afterwards Loofah powder is minced, bacillus thuringiensis, geotrichum candidum, fermentative microflora, glucose syrup and gross weight 1-2 water mix and blend 1.1-1.5h to all with, the yeast mixture obtaining Vegetable Sponge of Luffa loading is for subsequent use; At Loofah powder fiber, zinc gluconate, chitosan, acidic amino acid and gross weight 1-2 water is doubly blended in 90-95 DEG C, moderate-speed mixer 1-2h is to even, and it is for subsequent use that cooling draught drying is prepared into substratum porous amendment;
(2) by wormcast, the dregs of a decoction, manioc waste, bean dregs, spiral algae-residue, vegetation grass roots slag, the mixing of quartz sand slag, put into pulverizer, at pulverizing 20-30min, put into cryogenic high pressure decompressor expansion process 30-40min afterwards, obtain composite nutrition powder for subsequent use; By ganoderan, baking powder, Sodium.alpha.-hydroxypropionate, coarse whiting, L-sodium mixing, and add gross weight 2-3 water mixing and stirring doubly, obtain nutritive medium for subsequent use;
(3) by Chinese fir wood chip, sterilizing natural pond liquid, composite nutrition powder, steamed stuffed bun bacterium powder, nutritive medium, substratum amendment and gross weight 3-5 water mixing and stirring doubly, add the yeast mixture that a semiactive Vegetable Sponge of Luffa loads, at 18-23 DEG C of progressively intensification aerobic fermentation 12-15d; Adding the yeast mixture of residue one semiactive Vegetable Sponge of Luffa loading, after stirring, sealed fermenting 8-10d under 23-18 DEG C progressively cooling conditions; Obtain fermention medium for subsequent use;
(4) fermention medium is adopted centrifuge dehydration technology dewater to moisture content be the 60-63% of gross weight, pack, and in the high-temperature steam of 100 DEG C first ultrasonic assistant autoclaving 1.7-2h, at ultrasonic assistant low pressure sterilizing 0.75-1h, to obtain final product.
Advantage of the present invention is:
The present invention adopts Chinese fir wood chip to be equipped with other organic-inorganic material to prepare Stropharia rugoso-annulata substratum, nutritious, avoids the utilization of a large amount of agriculture chemical; Substratum porous amendment prepared by the yeast mixture simultaneously adopting Vegetable Sponge of Luffa to load and Vegetable Sponge of Luffa loading zinc gluconate, chitosan, acidic amino acid, undertaken by the decomposition of fermentation on the one hand, have adjusted medium nutrient content, on the other hand by porous amendment, have adjusted the absorption profiles of nutritive element and the hydrophobic problem of water conservation of substratum, the growth for Stropharia rugoso-annulata provides good environment and nutritive substance; The not only cultivation period of substratum cultivation of the present invention is short, and output is high, and the Stropharia rugoso-annulata of production is nutritious, delicious meat.
Embodiment
A kind of Stropharia rugoso-annulata high biological activity efficient culture medium, comprises following parts by weight of component: glucose syrup 3, ganoderan 3, Sodium.alpha.-hydroxypropionate 2, wormcast 12, baking powder 1.4, Chinese fir wood chip 60, bean dregs 30, manioc waste 15, sterilizing natural pond liquid 15, coarse whiting 1.3, L-Glu sodium 0.9, vegetation grass roots slag 20, quartz sand 15, the dregs of a decoction 6, bacillus thuringiensis 0.7, geotrichum candidum 0.9, spiral algae-residue 16, fermentative microflora 1.6, zinc gluconate 1, chitosan 2, acidic amino acid 2.4, steamed stuffed bun bacterium powder 2, Vegetable Sponge of Luffa 15, loofah fiber 5, appropriate water.
A kind of Stropharia rugoso-annulata high biological activity efficient culture medium preparation method, comprises the following steps:
(1) Vegetable Sponge of Luffa is put into pulverizer and pulverize 2min, obtain Loofah powder to mince, afterwards Loofah powder is minced, bacillus thuringiensis, geotrichum candidum, fermentative microflora, glucose syrup and gross weight 1 water mix and blend 1.1h to all with, the yeast mixture obtaining Vegetable Sponge of Luffa loading is for subsequent use; At the water of Loofah powder fiber, zinc gluconate, chitosan, acidic amino acid and gross weight 1 times is blended in 90 DEG C, moderate-speed mixer 1h is to even, and it is for subsequent use that cooling draught drying is prepared into substratum porous amendment;
(2) by wormcast, the dregs of a decoction, manioc waste, bean dregs, spiral algae-residue, vegetation grass roots slag, the mixing of quartz sand slag, put into pulverizer, at pulverizing 20min, put into cryogenic high pressure decompressor expansion process 30min afterwards, obtain composite nutrition powder for subsequent use; By ganoderan, baking powder, Sodium.alpha.-hydroxypropionate, coarse whiting, the mixing of L-Glu sodium, and add the water mixing and stirring of gross weight 2 times, obtain nutritive medium for subsequent use;
(3) by the water mixing and stirring of Chinese fir wood chip, sterilizing natural pond liquid, composite nutrition powder, steamed stuffed bun bacterium powder, nutritive medium, substratum amendment and gross weight 3 times, add the yeast mixture that a semiactive Vegetable Sponge of Luffa loads, at 18 DEG C of progressively intensification aerobic fermentation 12d; Adding the yeast mixture of residue one semiactive Vegetable Sponge of Luffa loading, after stirring, sealed fermenting 8d under 23 DEG C progressively cooling conditions; Obtain fermention medium for subsequent use;
(4) fermention medium is adopted centrifuge dehydration technology dewater to moisture content be 60% of gross weight, pack, and in the high-temperature steam of 100 DEG C first ultrasonic assistant autoclaving 1.7h, at ultrasonic assistant low pressure sterilizing 0.75h, to obtain final product.
Claims (2)
1. a Stropharia rugoso-annulata high biological activity efficient culture medium, it is characterized in that, comprise following parts by weight of component: glucose syrup 3-4, ganoderan 3-4, Sodium.alpha.-hydroxypropionate 2-3, wormcast 12-14, baking powder 1.4-1.6, Chinese fir wood chip 60-70, bean dregs 30-32, manioc waste 15-17, sterilizing natural pond liquid 15-20, coarse whiting 1.3-1.6, L-sodium 0.9-1.1, vegetation grass roots slag 20-30, quartz sand 15-17, dregs of a decoction 6-7, bacillus thuringiensis 0.7-0.8, geotrichum candidum 0.9-1, spiral algae-residue 16-18, fermentative microflora 1.6-1.9, zinc gluconate 1-1.4, chitosan 2-3, acidic amino acid 2.4-2.6, steamed stuffed bun bacterium powder 2-3, Vegetable Sponge of Luffa 15-17, loofah fiber 5-6, appropriate water.
2. a Stropharia rugoso-annulata high biological activity efficient culture medium preparation method, is characterized in that, comprise the following steps:
(1) Vegetable Sponge of Luffa is put into pulverizer and pulverize 2-3min, obtain Loofah powder to mince, afterwards Loofah powder is minced, bacillus thuringiensis, geotrichum candidum, fermentative microflora, glucose syrup and gross weight 1-2 water mix and blend 1.1-1.5h to all with, the yeast mixture obtaining Vegetable Sponge of Luffa loading is for subsequent use; At Loofah powder fiber, zinc gluconate, chitosan, acidic amino acid and gross weight 1-2 water is doubly blended in 90-95 DEG C, moderate-speed mixer 1-2h is to even, and it is for subsequent use that cooling draught drying is prepared into substratum porous amendment;
(2) by wormcast, the dregs of a decoction, manioc waste, bean dregs, spiral algae-residue, vegetation grass roots slag, the mixing of quartz sand slag, put into pulverizer, at pulverizing 20-30min, put into cryogenic high pressure decompressor expansion process 30-40min afterwards, obtain composite nutrition powder for subsequent use; By ganoderan, baking powder, Sodium.alpha.-hydroxypropionate, coarse whiting, L-sodium mixing, and add gross weight 2-3 water mixing and stirring doubly, obtain nutritive medium for subsequent use;
(3) by Chinese fir wood chip, sterilizing natural pond liquid, composite nutrition powder, steamed stuffed bun bacterium powder, nutritive medium, substratum amendment and gross weight 3-5 water mixing and stirring doubly, add the yeast mixture that a semiactive Vegetable Sponge of Luffa loads, at 18-23 DEG C of progressively intensification aerobic fermentation 12-15d; Adding the yeast mixture of residue one semiactive Vegetable Sponge of Luffa loading, after stirring, sealed fermenting 8-10d under 23-18 DEG C progressively cooling conditions; Obtain fermention medium for subsequent use;
(4) fermention medium is adopted centrifuge dehydration technology dewater to moisture content be the 60-63% of gross weight, pack, and in the high-temperature steam of 100 DEG C first ultrasonic assistant autoclaving 1.7-2h, at ultrasonic assistant low pressure sterilizing 0.75-1h, to obtain final product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105777404A (en) * | 2016-05-25 | 2016-07-20 | 广德县富丰蔬菜专业合作社 | Plant culture medium and preparation method thereof |
| CN113557909A (en) * | 2021-08-31 | 2021-10-29 | 吉林农业科技学院 | Morchella cultivation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104109011A (en) * | 2014-07-22 | 2014-10-22 | 肥东县丰宝种养殖有限责任公司 | Shiitake culture medium prepared from wood dust and preparation method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104109011A (en) * | 2014-07-22 | 2014-10-22 | 肥东县丰宝种养殖有限责任公司 | Shiitake culture medium prepared from wood dust and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105777404A (en) * | 2016-05-25 | 2016-07-20 | 广德县富丰蔬菜专业合作社 | Plant culture medium and preparation method thereof |
| CN113557909A (en) * | 2021-08-31 | 2021-10-29 | 吉林农业科技学院 | Morchella cultivation method |
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