CN106434364A - Production, preservation and application technology for liquid subpackaged strain - Google Patents
Production, preservation and application technology for liquid subpackaged strain Download PDFInfo
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- CN106434364A CN106434364A CN201610829601.2A CN201610829601A CN106434364A CN 106434364 A CN106434364 A CN 106434364A CN 201610829601 A CN201610829601 A CN 201610829601A CN 106434364 A CN106434364 A CN 106434364A
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- liquid
- strain
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- liquid strain
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- 239000007788 liquid Substances 0.000 title claims abstract description 63
- 238000004321 preservation Methods 0.000 title claims abstract description 24
- 238000005516 engineering process Methods 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 8
- 235000016709 nutrition Nutrition 0.000 claims description 12
- 230000009469 supplementation Effects 0.000 claims description 10
- 241000233866 Fungi Species 0.000 abstract description 8
- 230000001954 sterilising effect Effects 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 6
- 238000011081 inoculation Methods 0.000 abstract description 4
- 238000007789 sealing Methods 0.000 abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 238000012856 packing Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 235000015872 dietary supplement Nutrition 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 12
- 238000000855 fermentation Methods 0.000 description 12
- 230000004151 fermentation Effects 0.000 description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 210000000582 semen Anatomy 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000221377 Auricularia Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 150000002333 glycines Chemical class 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a production, preservation and application technology for a liquid subpackaged strain. A certain amount of nutritional supplement liquid obtained after sterilization is added into cultured liquid strain, then the liquid strain is subpackaged into sterilized packing materials under the aseptic condition and can be preserved for 2 months at normal temperature after sealing, the low-temperature preservation effect is better, and the preservation time is longer. When the strain is used, the sealing part is treated well under the aseptic condition, and the strain can be directly poured into a culture bag needing inoculation; a special liquid strain inoculation tool can be used also, conventional culture is carried out after inoculation, and the using effect is the same as that of a conventional liquid strain. Use cost of the liquid strain is greatly reduced, which is more beneficial for application and popularization of the liquid strain. The production, preservation and application technology is new breakthrough in subpackge, preservation and application of the liquid strain and is bound to further promote development of the liquid strain and edible and medical fungus industry.
Description
Technical field
The invention belongs to edible and medical fungi liquid spawn production technical field is and in particular to a kind of production of liquid subpackage strain
Preservation application technology.
Background technology
At present, on edible and medical fungi produces liquid spawn to be commonly used, liquid fungus seed and application technology become
Ripe, particularly it is widely used on factorial praluction.
Using liquid spawn, strain low production cost, strain quality be easily controlled, it is fast to inoculate sprouting, bacterium bag pollution rate is low,
Just with straight-lined manufacturing, it is especially suitable for large-scale production and factorial praluction.
Also there are a lot of problems in small-scale peasant household production and application liquid spawn, be present technology first, liquid spawn
Need to be in blank stage using subpackage preservation technology as early as possible after culture is good, therefore peasant household wants to be accomplished by using liquid spawn
Buy fermentation tank and need the liquid fungus seed technology of association's specialty and strain purity detecting technology could use upper liquid
Bacterium；And the price comparison of fermentation tank is high and utilization rate is too low, cause the wasting of resources；Due to producing liquid spawn using fermentation tank
Higher to the competency profiling of operator and so that part mushroom grower cannot be used.
The present invention by aseptic subpackaged, nutritional supplementation liquid the interpolation of cultured liquid spawn, so that liquid spawn is existed
Preserve 2 months under room temperature, mycelia survival rate more than 97%, peasant household can be made need not to grasp liquid fermentation not buying fermentation tank
The liquid spawn of technology equally use.
Content of the invention
The present invention is directed to the problems referred to above, provides one kind can be with subpackage, and preservation term length need not buy Zymolysis Equipment, facilitates mushroom grower
The liquid spawn production application technology using.
For achieving the above object, the present invention adopts the following technical scheme that.
Conventional method produces edible and medical fungi liquid spawn, standby after culture is good.
Configuration nutritional supplementation liquid：Conventional method configures strain cultivation liquid, and concentration requirement is the 1 of normal productive culture liquid
Times, the formula that formula can produce with strain, it is desirable that using the formula with the formula variation of spawn culture, as long as can train
The formula of foster same class strain is just permissible, and the nutritional supplementation liquid effect using the formula of differentiation is preferable, sterilizes standby after configuration is good
With.
The sterilizing of nutritional supplementation liquid will use the little sterilization tank with the aseptic connection of fermentation tank.
Select packing material：Can from vial, high temperature resistant north material bottle or high-temperature resistance plastice bag can, sterilize standby
With.
Aseptic subpackaged：Aseptically cultured liquid bacteria is dispensed into together with nutritional supplementation liquid the bag of bacterium of having gone out
Lid or mouth is sealed, the 5-10% that subpackage ratio nutritional solution accounts for strain amount is preferable in dress thing.
The aging fast basic reason of liquid spawn is that when strain cultivation is good, mycelia almost 99% make use of
The nutrition of culture medium, and cultured liquid spawn energetic nonnutritive material supplement in the case of will rapidly aging from
Molten.The present invention supplements certain nutrient substance airtight sealing preservation by subpackage, and no oxygen supply reduces the life of mycelia
Activity, therefore under normal temperature condition, the shelf-life can reach 2 months.
The preservation of fluid present invention strain, the strain after subpackage wants lucifuge preservation under room temperature, if any low-temperature preservation conditional effect
The more preferably preservation time can be longer.
The application of fluid present invention strain, handles strain bottleneck or bag mouth well by sterile working's code, shakes up liquid spawn
Uncap afterwards or opening after can directly pour in bacterium bag to be inoculated can also be using special liquid spawn inoculating tool, inoculation
, with normal liquid bacteria, cultural method is conventional for amount.
The culture medium nutritional supplementation liquid of the present invention makes and produces sterile working's code that subpackage follows edible and medical fungi production.
The subpackage liquid spawn of the present invention has the advantage that：1st, long shelf-life.2nd, easy to use, ready access upon use.3rd, become
Basis is low not to need special technology：Peasant household can be special without grasping to save great amount of investment using liquid bacteria without purchase fermentation tank
The liquid fungus seed technology of industry can be so that use liquid spawn.4th, convenient transport, is conducive to liquid spawn promotion and application.
Specific embodiment
Following examples are used for the present invention is described, but do not constitute any restriction to the present invention.
Embodiment：Auricularia liquid subpackage strain.
The culture of Auricularia liquid spawn, using 200 liters of liquid fermentation tank of culture amount, fermentation culture formula of liquid：Semen Maydiss
Powder 2%, glucose 2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.075%, pH value 5-6, configure fermentation liquid 200L, according to a conventional method
Fermentation culture is standby after fermenting.
Configuration nutritional supplementation liquid：From the nutritional supplementation formula of liquid with culture formula variation, formula is：Semen Glycines powder 1.5%,
Glucose 2%, Semen Maydis powder 1%, yeast powder 0.2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, because concentration will double configuration
When consumption double, configure Additional nutrient solution 20L, in little sterilization tank sterilize after standby, little sterilization tank will with spawn fermentation tank no
Bacterium links.
From high temperature resistant north material bottle, north material bottle cap upper bottle cover, then carry out autoclaving, standby after sterilizing.
Subpackage：The nutritional supplementation liquid preparing aseptically is mixed with cultured liquid bacteria, after mix homogeneously
Aseptically it is filled to and has gone out bacterium in plastic bottle, then cover tightly lid preservation standby.
Preservation：Can be had ready conditions best low-temperature preservation 2-5 DEG C with preservation under room temperature.
Use：Prepare bacterium bag according to a conventional method, after sterilizing cooling aseptically, with 75% alcohol washes subpackage
At the bottle cap of seed bottle, shake up liquid spawn and then uncap and pour 10 milliliters about of liquid spawn into bacterium bag into, conventional after sealing
Culture.Visible strain is sprouted within general 24 hours, grows energetic rapidly after sprouting, fast growth and use conventional liq strain
Effect the same.
Claims (2)
1. the production preservation application technology of a kind of liquid subpackage strain, its special type is cultured liquid spawn to add necessarily
The nutritional supplementation liquid of amount and then carry out aseptic subpackaged, can be used with the preservation regular hour after subpackage.
2. a kind of production preservation application technology of liquid subpackage strain according to claim 1 is it is characterised in that can be normal
Warm preservation 2 months, the low-temperature preservation effect more preferable time is longer, and using effect is as conventional liq strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610829601.2A CN106434364B (en) | 2016-09-19 | 2016-09-19 | Production, preservation and application technology of liquid subpackaged strains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610829601.2A CN106434364B (en) | 2016-09-19 | 2016-09-19 | Production, preservation and application technology of liquid subpackaged strains |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106434364A true CN106434364A (en) | 2017-02-22 |
| CN106434364B CN106434364B (en) | 2021-06-08 |
Family
ID=58168172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610829601.2A Active CN106434364B (en) | 2016-09-19 | 2016-09-19 | Production, preservation and application technology of liquid subpackaged strains |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106434364B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1073828A (en) * | 1992-07-21 | 1993-07-07 | 方可武 | A kind of storage method of edible fungi liquid strain |
| CN1733899A (en) * | 2005-08-12 | 2006-02-15 | 中国农业大学 | Edible mushroom liquid bacteria preservation method |
| CN102106233A (en) * | 2009-12-29 | 2011-06-29 | 上海市农业科学院 | Method for preserving strains of ectomycorrhizal fungi |
| CN103392503A (en) * | 2013-07-18 | 2013-11-20 | 贵州省生物研究所 | Methods for preparing and preserving dictyophora rubrovolvata culture |
| CN103922833A (en) * | 2013-01-16 | 2014-07-16 | 孙君莲 | Black fungus liquid strain culture medium and preparation method thereof |
| CN104285677A (en) * | 2014-10-30 | 2015-01-21 | 武汉岁岁丰农业科技开发有限公司 | Method for making edible mushroom pegwood strain |
| CN105753531A (en) * | 2015-12-30 | 2016-07-13 | 辽宁师范大学 | Cultivation medium for liquid strains of black fungi and method for preparing cultivation medium |
-
2016
- 2016-09-19 CN CN201610829601.2A patent/CN106434364B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1073828A (en) * | 1992-07-21 | 1993-07-07 | 方可武 | A kind of storage method of edible fungi liquid strain |
| CN1733899A (en) * | 2005-08-12 | 2006-02-15 | 中国农业大学 | Edible mushroom liquid bacteria preservation method |
| CN102106233A (en) * | 2009-12-29 | 2011-06-29 | 上海市农业科学院 | Method for preserving strains of ectomycorrhizal fungi |
| CN103922833A (en) * | 2013-01-16 | 2014-07-16 | 孙君莲 | Black fungus liquid strain culture medium and preparation method thereof |
| CN103392503A (en) * | 2013-07-18 | 2013-11-20 | 贵州省生物研究所 | Methods for preparing and preserving dictyophora rubrovolvata culture |
| CN104285677A (en) * | 2014-10-30 | 2015-01-21 | 武汉岁岁丰农业科技开发有限公司 | Method for making edible mushroom pegwood strain |
| CN105753531A (en) * | 2015-12-30 | 2016-07-13 | 辽宁师范大学 | Cultivation medium for liquid strains of black fungi and method for preparing cultivation medium |
Non-Patent Citations (2)
| Title |
|---|
| 夏温澍: "黑木耳菌种分离培养与保藏的研究总结", 《湖北农业科学》 * |
| 邱奉同等: "《食用菌栽培技术》", 31 July 2014, 山东人民出版社 * |
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| CN106434364B (en) | 2021-06-08 |
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Effective date of registration: 20210428 Address after: Group 6, Sanjiazi Village, Jiangnan Township, Fengman District, Jilin City, 132000 Jilin Province Applicant after: Jilin Shenzhi Technology Development Co.,Ltd. Address before: Six agency Jiang Nan Xiang Zi Cun, Fengman District Sanjia 132013 Jilin city of Jilin province HSBC Technology Co. Ltd. Applicant before: Yu Hui |
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Address after: Room 1415, 14th Floor, Innovation Center, High tech Industrial Development Zone, Yandu District, Yancheng City, Jiangsu Province, 224000 Patentee after: Yancheng Shenmu Technology Development Co.,Ltd. Address before: 132000 group 6, Sanjiazi Village, Jiangnan Township, Fengman District, Jilin City, Jilin Province Patentee before: Jilin Shenzhi Technology Development Co.,Ltd. |
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