CN102674928A - Mushroom mycelium solid medium and method for culturing mushroom mycelium by using same - Google Patents
Mushroom mycelium solid medium and method for culturing mushroom mycelium by using same Download PDFInfo
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- CN102674928A CN102674928A CN2012101400962A CN201210140096A CN102674928A CN 102674928 A CN102674928 A CN 102674928A CN 2012101400962 A CN2012101400962 A CN 2012101400962A CN 201210140096 A CN201210140096 A CN 201210140096A CN 102674928 A CN102674928 A CN 102674928A
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- solid medium
- mushroom mycelium
- shiitake mushroom
- mushroom hypha
- bacterium
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Abstract
The invention provides a mushroom mycelium solid medium and a method for culturing mushroom mycelium by using the same. The mushroom mycelium solid medium is prepared from the following raw materials proportionally: 35-42% of bagasse, 3.5-7% of rice bran, 3.5-7% of corn starch, 0.4-0.6% of gypsum, 0.4-0.6% of sugar, 0.4-0.6% of salt and 45-55% of water. The method for culturing the mushroom mycelium by using the mushroom mycelium solid medium comprises the following steps of: weighing the raw materials in proportion, and mixing the raw materials to prepare into the mushroom mycelium solid medium; bagging and sterilizing the prepared mushroom mycelium solid medium; inoculating the mushroom mycelium on the solid medium; and placing the solid medium in a spawn-running room for carrying out spawn running. The solid medium provided by the invention is used for culturing the mushroom mycelium, so that the production technology is simple and the production cost is low; and the contents of lentinan and various types of active substances in the cultured mushroom mycelium are high, so that the mushroom mycelium solid medium and the method are especially suitable for industrialized production and application.
Description
Technical field
The present invention relates to the culture technique field of shiitake mushroom hypha, be specifically related to a kind of shiitake mushroom hypha solid medium and cultivate the method for shiitake mushroom hypha with it.
Background technology
In Edible Fungi; Produce sporophore and need miscellaneous technology and long time; Along with the continuous development of scientific research, it is found that the contained various nutritive ingredients of mycelium are similar with sporophore, thereby replace sporophore to carry out various production applications through the culture bacteria filament.Shiitake mushroom hypha is rich in lentinan, multiple amino acids, vitamin E, various trace elements and various wholesome nutrient substance, can be processed into the nutritive food or the foodstuff additive of various ways, can also be as raw materials such as medicine, healthcare products, makeup.Shiitake mushroom hypha is mainly cultivated through liquid nutrient medium in producing at present, although this method has certain advantage, in case in production application, there are deficiencies such as facility investment is big, the complex process pollution loss is serious.
Summary of the invention
The technical problem that the present invention solved provides a kind of shiitake mushroom hypha solid medium and cultivates the method for shiitake mushroom hypha with it; Production technique with this solid medium cultivation shiitake mushroom hypha is simple, production cost is low; And the lentinan of the shiitake mushroom hypha of being cultivated and various activity substance content are high, are specially adapted to suitability for industrialized production and use.
The technical scheme that the present invention adopted is: a kind of shiitake mushroom hypha solid medium is formed by the feedstock production of by weight following ratio: bagasse 35～42%, rice bran 3.5～7%; Semen Maydis powder 3.5～7%, gypsum 0.4～0.6%, sugar 0.4～0.6%; Salt 0.4～0.6%, water 45～55%.
The method that the said shiitake mushroom hypha solid medium of a kind of usefulness is cultivated shiitake mushroom hypha may further comprise the steps:
The first step: carry out uniform mixing after said raw material taken by weighing in proportion, make the shiitake mushroom hypha solid medium;
Second step: the shiitake mushroom hypha solid medium that makes is packed, sterilize then;
The 3rd step: the mushroom fungi is seeded on the shiitake mushroom hypha solid medium after the sterilization;
The 4th step: postvaccinal shiitake mushroom hypha solid medium is moved into indoor cultivation of a bacterium send out a bacterium, send out a bacterium temperature and be controlled to be 23～25 ℃, sending out the bacterium time is 90～120 days, and is aided with illumination in last 10～20 days that send out bacterium.
The invention has the beneficial effects as follows: the main raw material that 1, the shiitake mushroom hypha solid medium adopts among the present invention is a bagasse, not only realizes the comprehensive utilization of refuse, helps protecting ecological resources, and can reduce the raw materials for production cost; 2, cultivate shiitake mushroom hypha with solid medium according to the invention; Its technology of producing shiitake mushroom hypha is simple, production cost is low; And the lentinan of the shiitake mushroom hypha of being cultivated and various activity substance content are high; Be applicable to the suitability for industrialized production application, help promoting the industrialization comprehensive utilization of shiitake mushroom hypha; 3, cultivate shiitake mushroom hypha with solid medium according to the invention,, when the solid medium in indivedual bags wherein is contaminated, remove easily, can not cause heavy losses because solid medium is packed.
Embodiment
Below in conjunction with specific embodiment the present invention is further specified.
Embodiment 1
A kind of shiitake mushroom hypha solid medium is formed by following feedstock production: bagasse 35 ㎏, rice bran 7 ㎏, Semen Maydis powder 7 ㎏, gypsum 0.6 ㎏, sugared 0.6 ㎏, salt 0.6 ㎏, water 49.2 ㎏.
Method with said solid medium is cultivated shiitake mushroom hypha may further comprise the steps:
The first step: take by weighing bagasse 35 ㎏, rice bran 7 ㎏, Semen Maydis powder 7 ㎏, gypsum 0.6 ㎏, sugared 0.6 ㎏, salt 0.6 ㎏, water 49.2 ㎏ and carry out uniform mixing, make the shiitake mushroom hypha solid medium;
Second step: the shiitake mushroom hypha solid medium is packed by every bag 1.8 ㎏, carry out sterilising treatment then;
The 3rd step: the mushroom fungi is seeded on the shiitake mushroom hypha solid medium after the sterilization;
The 4th step: postvaccinal shiitake mushroom hypha solid medium is moved into indoor cultivation of a bacterium send out a bacterium, send out a bacterium temperature and be controlled to be 23～25 ℃, sending out the bacterium time is 90 days, and is aided with illumination in last 10 days that send out bacterium.
Embodiment 2
A kind of shiitake mushroom hypha solid medium is formed by following feedstock production: bagasse 42 ㎏, rice bran 3.5 ㎏, Semen Maydis powder 3.5 ㎏, gypsum 0.4 ㎏, sugared 0.4 ㎏, salt 0.4 ㎏, water 49.8 ㎏.
Method with said solid medium is cultivated shiitake mushroom hypha may further comprise the steps:
The first step: bagasse 42 ㎏, rice bran 3.5 ㎏, Semen Maydis powder 3.5 ㎏, gypsum 0.4 ㎏, sugared 0.4 ㎏, salt 0.4 ㎏, water 49.8 ㎏ carry out uniform mixing, make the shiitake mushroom hypha solid medium;
Second step: the shiitake mushroom hypha solid medium is packed by every bag 1.8 ㎏, carry out sterilising treatment then;
The 3rd step: the mushroom fungi is seeded on the shiitake mushroom hypha solid medium after the sterilization;
The 4th step: postvaccinal shiitake mushroom hypha solid medium is moved into indoor cultivation of a bacterium send out a bacterium, send out a bacterium temperature and be controlled to be 23～25 ℃, sending out the bacterium time is 120 days, and is aided with illumination in last 20 days that send out bacterium.
Embodiment 3
A kind of shiitake mushroom hypha solid medium is formed by following feedstock production: bagasse 40 ㎏, rice bran 7 ㎏, Semen Maydis powder 6.5 ㎏, gypsum 0.5 ㎏, sugared 0.5 ㎏, salt 0.5 ㎏, water 45 ㎏.
Method with said solid medium is cultivated shiitake mushroom hypha may further comprise the steps:
The first step: take by weighing bagasse 40 ㎏, rice bran 7 ㎏, Semen Maydis powder 6.5 ㎏, gypsum 0.5 ㎏, sugared 0.5 ㎏, salt 0.5 ㎏, water 45 ㎏ and carry out uniform mixing, make the shiitake mushroom hypha solid medium;
Second step: the shiitake mushroom hypha solid medium is packed by every bag 1.8 ㎏, carry out sterilising treatment then;
The 3rd step: the mushroom fungi is seeded on the shiitake mushroom hypha solid medium after the sterilization;
The 4th step: postvaccinal shiitake mushroom hypha solid medium is moved into indoor cultivation of a bacterium send out a bacterium, send out a bacterium temperature and be controlled to be 23～25 ℃, sending out the bacterium time is 100 days, and is aided with illumination in last 15 days that send out bacterium.
Embodiment 4
A kind of shiitake mushroom hypha solid medium is formed by following feedstock production: bagasse 36 ㎏, rice bran 4 ㎏, Semen Maydis powder 3.5 ㎏, gypsum 0.5 ㎏, sugared 0.5 ㎏, salt 0.5 ㎏, water 55 ㎏.
Method with said solid medium is cultivated shiitake mushroom hypha may further comprise the steps:
The first step: take by weighing bagasse 36 ㎏, rice bran 4 ㎏, Semen Maydis powder 3.5 ㎏, gypsum 0.5 ㎏, sugared 0.5 ㎏, salt 0.5 ㎏, water 55 ㎏ and carry out uniform mixing, make the shiitake mushroom hypha solid medium;
Second step: the shiitake mushroom hypha solid medium is packed by every bag 1.8 ㎏, carry out sterilising treatment then;
The 3rd step: the mushroom fungi is seeded on the shiitake mushroom hypha solid medium after the sterilization;
The 4th step: postvaccinal shiitake mushroom hypha solid medium is moved into indoor cultivation of a bacterium send out a bacterium, send out a bacterium temperature and be controlled to be 23～25 ℃, sending out the bacterium time is 110 days, and is aided with illumination in last 15 days that send out bacterium.
Claims (2)
1. shiitake mushroom hypha solid medium is characterized in that being formed by the feedstock production of by weight following ratio: bagasse 35～42%, rice bran 3.5～7%; Semen Maydis powder 3.5～7%, gypsum 0.4～0.6%, sugar 0.4～0.6%; Salt 0.4～0.6%, water 45～55%.
2. the method for cultivating shiitake mushroom hypha with the said solid medium of claim 1 is characterized in that may further comprise the steps:
The first step: carry out uniform mixing after said raw material taken by weighing in proportion, make the shiitake mushroom hypha solid medium;
Second step: the shiitake mushroom hypha solid medium that makes is packed, sterilize then;
The 3rd step: the mushroom fungi is seeded on the shiitake mushroom hypha solid medium after the sterilization;
The 4th step: postvaccinal shiitake mushroom hypha solid medium is moved into indoor cultivation of a bacterium send out a bacterium, send out a bacterium temperature and be controlled to be 23～25 ℃, sending out the bacterium time is 90～120 days, and is aided with illumination in last 10～20 days that send out bacterium.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101400962A CN102674928A (en) | 2012-05-09 | 2012-05-09 | Mushroom mycelium solid medium and method for culturing mushroom mycelium by using same |
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| CN2012101400962A CN102674928A (en) | 2012-05-09 | 2012-05-09 | Mushroom mycelium solid medium and method for culturing mushroom mycelium by using same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404732A (en) * | 2013-08-15 | 2013-11-27 | 哈尔滨伟平科技开发有限公司 | Preparation method of feed additive for preventing and treating soft-shelled turtle soft-shell disease |
| CN105237149A (en) * | 2015-08-28 | 2016-01-13 | 颍上县鸿涛菌业专业合作社 | Culture medium prepared by fermenting liquid materials and preparation method thereof |
| CN106509842A (en) * | 2016-10-17 | 2017-03-22 | 福建农林大学 | Preparation method of antrodia camphorata nutriment |
| CN107285833A (en) * | 2017-07-17 | 2017-10-24 | 广西秀瑶姑生态农业科技有限公司 | A kind of culture medium for cultivating and cultural method for improving lentinan content |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391790A (en) * | 2001-06-15 | 2003-01-22 | 松茂有机肥料有限公司 | Cultivating method for mushroom |
| CN101514116A (en) * | 2008-02-22 | 2009-08-26 | 大叶大学 | Formula for culture medium for culturing mushrooms with bagasse |
-
2012
- 2012-05-09 CN CN2012101400962A patent/CN102674928A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391790A (en) * | 2001-06-15 | 2003-01-22 | 松茂有机肥料有限公司 | Cultivating method for mushroom |
| CN101514116A (en) * | 2008-02-22 | 2009-08-26 | 大叶大学 | Formula for culture medium for culturing mushrooms with bagasse |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404732A (en) * | 2013-08-15 | 2013-11-27 | 哈尔滨伟平科技开发有限公司 | Preparation method of feed additive for preventing and treating soft-shelled turtle soft-shell disease |
| CN105237149A (en) * | 2015-08-28 | 2016-01-13 | 颍上县鸿涛菌业专业合作社 | Culture medium prepared by fermenting liquid materials and preparation method thereof |
| CN106509842A (en) * | 2016-10-17 | 2017-03-22 | 福建农林大学 | Preparation method of antrodia camphorata nutriment |
| CN107285833A (en) * | 2017-07-17 | 2017-10-24 | 广西秀瑶姑生态农业科技有限公司 | A kind of culture medium for cultivating and cultural method for improving lentinan content |
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Application publication date: 20120919 |