CN105219586A - A kind of fermented type gas water beverage and preparation method thereof - Google Patents
A kind of fermented type gas water beverage and preparation method thereof Download PDFInfo
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- CN105219586A CN105219586A CN201510722731.1A CN201510722731A CN105219586A CN 105219586 A CN105219586 A CN 105219586A CN 201510722731 A CN201510722731 A CN 201510722731A CN 105219586 A CN105219586 A CN 105219586A
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Abstract
The invention discloses a kind of fermented type gas water beverage and preparation method thereof, this fermented type gas water beverage is in obtained beverage for 100%, and the mass percent of each component that this beverage packets contains is: Trisodium Citrate 0.1-0.3%; Lactobacillus bulgaricus 1-3%; Thermophilic bacteria 1-3%; Cereuisiae fermentum 1-3%; Heart of a lotus seed benevolence bud juice 31-32%; Pollen of Masson Pine extracting solution 59-60%; 55 type high fructose syrup 2-4%.Through preparing heart of a lotus seed benevolence bud juice, prepare Pollen of Masson Pine extracting solution, domesticated strain, determine drink formula, prepare fermented type gas water beverage 5 step and operate and get final product.Have that color is limpid, taste is strong fragrant, the feature of mellow in taste.
Description
Technical field
The invention belongs to food technology field, be specifically related to a kind of fermented type gas water beverage, also relate to the preparation method of this fermented type gas water beverage simultaneously.
Background technology
Gas water beverage is a kind of beverage containing low alcohol, color is similar to beer and slightly takes on a red color, moderately sour and sweet, its ethanol content only has about 1%, owing to having good fermented flavour and raw material local flavor, the macromolecular substance such as protein, starch in raw material can be made to degrade and improve digestibility, is be subject to the soft drink that human consumer all over the world extensively likes.
In recent years, along with improving constantly of people's living standard, the function fermenting beverage of rich in nutritive value becomes one of important directions of beverage processing development gradually, and related application also more increases, but its formula and local flavor are had nothing in common with each other.
Containing the nutritive ingredient such as protein, fat, carbohydrate, robust fibre, mineral calcium, phosphorus, iron, VITMAIN B1, Lin Suanna Vitamin B2 Sodium Phosphate, nicotinic acid, starch, leucine, arginine, Methionin, tyrosine, lipid acid, seed of jog's tears ester, seed of jog's tears oil, Sitosterol, alkaloid in the seed of Job's tears, there is protection blood vessel, improve microcirculation, reduction blood sugar and blood fat, raising insulin receptor susceptibility effect.
Be distributed widely in the Pinus massoniana Lamb in scope on the south the Yunnan-Guizhou Plateau, the Qinling Mountains, Huaihe River, growth area occupies first of national softwood forest.Various nutrients containing needed by human body in its pollen; comprise 22 seed amino acids, 15 kinds of VITAMIN, 30 several mineral materials and unsaturated fatty acids, Yelkin TTS, flavonoid, antioxidant etc., have antifatigue, anti-ageing, protection liver and cardiovascular and cerebrovascular, raising immunity of human body itself effect.
Up to now, there is not yet with heart of a lotus seed Renhe Pollen of Masson Pine as main raw material prepares the relevant report of fermented type gas water beverage.
Summary of the invention
The object of the present invention is to provide that a kind of color is limpid, taste is strong fragrant, the fermented type gas water beverage of mellow in taste.
Another object of the present invention is to the preparation method that this fermented type gas water beverage is provided.
A kind of fermented type gas water beverage of the present invention, in obtained beverage for 100%, the mass percent of each component that this beverage packets contains is:
Trisodium Citrate 0.1-0.3%;
Lactobacillus bulgaricus 1-3%;
Thermophilic bacteria 1-3%;
Cereuisiae fermentum 1-3%;
Heart of a lotus seed benevolence bud juice 31-32%;
Pollen of Masson Pine extracting solution 59-60%;
55 type high fructose syrup 2-4%.
Above-mentioned a kind of fermented type gas water beverage, in obtained beverage for 100%, the mass percent of each component that this beverage packets contains is:
Trisodium Citrate 0.2%;
Lactobacillus bulgaricus 2%;
Thermophilic bacteria 2%;
Cereuisiae fermentum 2%;
Heart of a lotus seed benevolence bud juice 3l.72%;
Pollen of Masson Pine extracting solution 59.08%;
55 type high fructose syrups 3%.
Above-mentioned a kind of fermented type gas water beverage, wherein: described heart of a lotus seed benevolence bud juice is prepared by the mass ratio of 1:3 by black shell heart of a lotus seed benevolence and gold heart of a lotus seed benevolence.
The preparation method of a kind of fermented type gas water beverage of the present invention, comprises the following steps:
(1) heart of a lotus seed benevolence bud juice is prepared:
Get after heart of a lotus seed benevolence soaks 20-25h in 25-35 DEG C of water and at room temperature sprout 2-3 days; Get germination heart of a lotus seed benevolence and dry moisture content at 45-55 DEG C, be crushed to 80-120 order, shell, obtain heart of a lotus seed benevolence bud powder; Add water, the mass ratio of heart of a lotus seed benevolence bud powder and water is 1:1, obtains heart of a lotus seed benevolence bud powder solution; Add the saccharifying enzyme saccharification of heart of a lotus seed benevolence bud powder solution quality 2-4%, filter, get filtrate and obtain heart of a lotus seed benevolence bud juice;
(2) Pollen of Masson Pine extracting solution is prepared:
Get Pollen of Masson Pine, clear water embathes rear filtration, and filter cake is added clear water by the mass ratio of 1:20-25, in 45-55 DEG C, ultrasonication 55-65min under 75-85% power, then stored refrigerated at being placed in 0-4 DEG C, obtain Pollen of Masson Pine extracting solution;
(3) domesticated strain:
Extracting degreasing breast prepares nutrient solution with the mass ratio that 9:1 press by Pollen of Masson Pine extracting solution, using the lactobacillus bulgaricus that activated and thermophilic bacteria as bacterial classification, accesses in nutrient solution by the inoculum size of nutrient solution volume ratio each 2%; At postvaccinal nutrient solution is placed in 40-45 DEG C, after nutrient solution solidifies, to prepare in the nutrient solution obtained by 8:2 mass ratio to skimming milk and Pollen of Masson Pine extracting solution by cultivating the strain inoculation that obtains, at being still placed in 40-45 DEG C, after nutrient solution solidifies, by that analogy, continue to cultivate, until the mass ratio of skimming milk and Pollen of Masson Pine extracting solution reaches 1:1; Finally at 37-42 DEG C, cultivate bacterial classification 11-13h separately with Pollen of Masson Pine extracting solution, the volume ratio of bacterial classification and Pollen of Masson Pine extracting solution is 1:20, obtains beverage lactobacillus bulgaricus and thermophilic bacteria;
(4) drink formula is determined:
Each raw material is taken by following quality proportioning:
Trisodium Citrate 0.1-0.3%, beverage lactobacillus bulgaricus 1-3%, beverage thermophilic bacteria 1-3%, cereuisiae fermentum 1-3%, heart of a lotus seed benevolence bud juice 31-32%, Pollen of Masson Pine extracting solution 59-60%, 55 type high fructose syrup 2-4%;
(5) fermented type gas water beverage is prepared:
Heart of a lotus seed benevolence bud juice and Pollen of Masson Pine extracting solution are added in fermentor tank, sterilizing, add beverage lactobacillus bulgaricus, beverage thermophilic bacteria and cereuisiae fermentum, in 35-40 DEG C of bottom fermentation 20-25h; Add Trisodium Citrate and 55 type high fructose syrups, pasteurization, obtains raw mixture; Add the chitosan of raw mixture quality 1% again as finings, to be precipitated completely after, filter, get filtrate, to obtain final product.
The preparation method of above-mentioned a kind of fermented type gas water beverage, wherein: after obtaining heart of a lotus seed benevolence bud powder in step (1), first can add the water of heart of a lotus seed benevolence bud opaque amount 8-10% and the yeast of 1-3% in heart of a lotus seed benevolence bud powder, by the kneading of heart of a lotus seed benevolence bud powder to bulk, in 25-35 DEG C of bottom fermentation 10-15h, then toast 15-25min at 170-180 DEG C, be cooled to 38-42 DEG C, mass ratio again by 1:1 after being crushed to 50-70 order adds water, and continues next-step operation.
The present invention compared with prior art; there is obvious beneficial effect; as can be known from the above technical solutions: fermented type gas water beverage provided by the present invention; the heart of a lotus seed benevolence bud juice and the Pollen of Masson Pine extracting solution that adopt rational proportion are raw material; possessed protection cardiovascular, improve microcirculation, protection liver, reduce blood sugar and blood fat, raising insulin receptor susceptibility; and the effect of energy antifatigue, regulating intestinal canal and immunologic function; products obtained therefrom fragrance is strong fragrant; local flavor, mellow in taste, color is limpid and taste is salubrious.The preparation method provided, 5 steps are divided progressively to carry out, heart of a lotus seed benevolence bud juice and the Pollen of Masson Pine extracting solution of delicious, high nutritive value can be obtained, the reasonable domestication of bacterial classification can guarantee again product fermentation completely also improving product taste, each technological step and processing condition are worked in coordination with mutually, have easy and simple to handle and be easy to promote feature.
Embodiment
embodiment 1
A kind of fermented type gas water beverage, comprises the following steps:
(1) heart of a lotus seed benevolence bud juice is prepared:
Get after heart of a lotus seed benevolence soaks 20h in 25 DEG C of water and at room temperature sprout 3 days; Get germination heart of a lotus seed benevolence and dry moisture content at 55 DEG C, be crushed to 120 orders, shell, obtain heart of a lotus seed benevolence bud powder; Add water, the mass ratio of heart of a lotus seed benevolence bud powder and water is 1:1, obtains heart of a lotus seed benevolence bud powder solution; Add the saccharifying enzyme saccharification of heart of a lotus seed benevolence bud powder solution quality 2%, filter, get filtrate and obtain heart of a lotus seed benevolence bud juice;
(2) Pollen of Masson Pine extracting solution is prepared:
Get Pollen of Masson Pine, clear water embathes rear filtration, and filter cake is added clear water by the mass ratio of 1:20, in 45 DEG C, ultrasonication 55min under 75% power, then stored refrigerated at being placed in 0 DEG C, obtain Pollen of Masson Pine extracting solution;
(3) domesticated strain:
Extracting degreasing breast prepares nutrient solution with the mass ratio that 9:1 press by Pollen of Masson Pine extracting solution, using the lactobacillus bulgaricus that activated and thermophilic bacteria as bacterial classification, accesses in nutrient solution by the inoculum size of nutrient solution volume ratio each 2%; At postvaccinal nutrient solution is placed in 40 DEG C, after nutrient solution solidifies, to prepare in the nutrient solution obtained by 8:2 mass ratio to skimming milk and Pollen of Masson Pine extracting solution by cultivating the strain inoculation that obtains, at being still placed in 40 DEG C, after nutrient solution solidifies, by that analogy, continue to cultivate, until the mass ratio of skimming milk and Pollen of Masson Pine extracting solution reaches 1:1; Finally at 37 DEG C, cultivate bacterial classification 13h separately with Pollen of Masson Pine extracting solution, the volume ratio of bacterial classification and Pollen of Masson Pine extracting solution is 1:20, obtains beverage lactobacillus bulgaricus and thermophilic bacteria;
(4) drink formula is determined:
Each raw material is taken by following quality proportioning:
Trisodium Citrate 0.3kg, beverage lactobacillus bulgaricus 1kg, beverage thermophilic bacteria 3kg, cereuisiae fermentum 3kg, heart of a lotus seed benevolence bud juice 31kg, Pollen of Masson Pine extracting solution 59.7kg, 55 type high fructose syrup 2kg;
(5) fermented type gas water beverage is prepared:
Heart of a lotus seed benevolence bud juice and Pollen of Masson Pine extracting solution are added in fermentor tank, sterilizing, add beverage lactobacillus bulgaricus, beverage thermophilic bacteria and cereuisiae fermentum, in 35 DEG C of bottom fermentation 20h; Add Trisodium Citrate and 55 type high fructose syrups, pasteurization, obtains raw mixture; Add the chitosan of raw mixture quality 1% again as finings, to be precipitated completely after, filter, get filtrate, to obtain final product.
embodiment 2
A kind of fermented type gas water beverage, comprises the following steps:
(1) heart of a lotus seed benevolence bud juice is prepared:
Get after heart of a lotus seed benevolence soaks 25h in 35 DEG C of water and at room temperature sprout 2 days; Get germination heart of a lotus seed benevolence and dry moisture content at 45 DEG C, be crushed to 80 orders, shell, obtain heart of a lotus seed benevolence bud powder; Add water, the mass ratio of heart of a lotus seed benevolence bud powder and water is 1:1, obtains heart of a lotus seed benevolence bud powder solution; Add the saccharifying enzyme saccharification of heart of a lotus seed benevolence bud powder solution quality 4%, filter, get filtrate and obtain heart of a lotus seed benevolence bud juice;
(2) Pollen of Masson Pine extracting solution is prepared:
Get Pollen of Masson Pine, clear water embathes rear filtration, and filter cake is added clear water by the mass ratio of 1:25, in 55 DEG C, ultrasonication 65min under 85% power, then stored refrigerated at being placed in 4 DEG C, obtain Pollen of Masson Pine extracting solution;
(3) domesticated strain:
Extracting degreasing breast prepares nutrient solution with the mass ratio that 9:1 press by Pollen of Masson Pine extracting solution, using the lactobacillus bulgaricus that activated and thermophilic bacteria as bacterial classification, accesses in nutrient solution by the inoculum size of nutrient solution volume ratio each 2%; At postvaccinal nutrient solution is placed in 45 DEG C, after nutrient solution solidifies, to prepare in the nutrient solution obtained by 8:2 mass ratio to skimming milk and Pollen of Masson Pine extracting solution by cultivating the strain inoculation that obtains, at being still placed in 45 DEG C, after nutrient solution solidifies, by that analogy, continue to cultivate, until the mass ratio of skimming milk and Pollen of Masson Pine extracting solution reaches 1:1; Finally at 42 DEG C, cultivate bacterial classification 11h separately with Pollen of Masson Pine extracting solution, the volume ratio of bacterial classification and Pollen of Masson Pine extracting solution is 1:20, obtains beverage lactobacillus bulgaricus and thermophilic bacteria;
(4) drink formula is determined:
Each raw material is taken by following quality proportioning:
Trisodium Citrate 0.1kg, beverage lactobacillus bulgaricus 3kg, beverage thermophilic bacteria 1kg, cereuisiae fermentum 1kg, heart of a lotus seed benevolence bud juice 31.9kg, Pollen of Masson Pine extracting solution 59kg, 55 type high fructose syrup 4kg;
(5) fermented type gas water beverage is prepared:
Heart of a lotus seed benevolence bud juice and Pollen of Masson Pine extracting solution are added in fermentor tank, sterilizing, add beverage lactobacillus bulgaricus, beverage thermophilic bacteria and cereuisiae fermentum, in 40 DEG C of bottom fermentation 25h; Add Trisodium Citrate and 55 type high fructose syrups, pasteurization, obtains raw mixture; Add the chitosan of raw mixture quality 1% again as finings, to be precipitated completely after, filter, get filtrate, to obtain final product.
embodiment 3
A preparation method for fermented type gas water beverage, comprises the following steps:
(1) heart of a lotus seed benevolence bud juice is prepared:
Get after heart of a lotus seed benevolence soaks 22.5h in 30 DEG C of water and at room temperature sprout 2.5 days; Get germination heart of a lotus seed benevolence and dry moisture content at 50 DEG C, be crushed to 100 orders, shell, obtain heart of a lotus seed benevolence bud powder; In heart of a lotus seed benevolence bud powder, add the water of heart of a lotus seed benevolence bud opaque amount 8% and the yeast of 3%, by the kneading of heart of a lotus seed benevolence bud powder to bulk, in 25 DEG C of bottom fermentation 15h, then toast 25min at 170 DEG C, be cooled to 38 DEG C, be crushed to 50 orders; Add water, the mass ratio of heart of a lotus seed benevolence bud powder and water is 1:1, obtains heart of a lotus seed benevolence bud powder solution; Add the saccharifying enzyme saccharification of heart of a lotus seed benevolence bud powder solution quality 3%, filter, get filtrate and obtain heart of a lotus seed benevolence bud juice;
(2) Pollen of Masson Pine extracting solution is prepared:
Get Pollen of Masson Pine, clear water embathes rear filtration, and filter cake is added clear water by the mass ratio of 1:22.5, in 50 DEG C, ultrasonication 60min under 80% power, then stored refrigerated at being placed in 2 DEG C, obtain Pollen of Masson Pine extracting solution;
(3) domesticated strain:
Extracting degreasing breast prepares nutrient solution with the mass ratio that 9:1 press by Pollen of Masson Pine extracting solution, using the lactobacillus bulgaricus that activated and thermophilic bacteria as bacterial classification, accesses in nutrient solution by the inoculum size of nutrient solution volume ratio each 2%; At postvaccinal nutrient solution is placed in 42.5 DEG C, after nutrient solution solidifies, to prepare in the nutrient solution obtained by 8:2 mass ratio to skimming milk and Pollen of Masson Pine extracting solution by cultivating the strain inoculation that obtains, at being still placed in 42.5 DEG C, after nutrient solution solidifies, by that analogy, continue to cultivate, until the mass ratio of skimming milk and Pollen of Masson Pine extracting solution reaches 1:1; Finally at 40 DEG C, cultivate bacterial classification 12h separately with Pollen of Masson Pine extracting solution, the volume ratio of bacterial classification and Pollen of Masson Pine extracting solution is 1:20, obtains beverage lactobacillus bulgaricus and thermophilic bacteria;
(4) drink formula is determined:
Each raw material is taken by following quality proportioning:
Trisodium Citrate 0.2kg, beverage lactobacillus bulgaricus 2kg, beverage thermophilic bacteria 2kg, cereuisiae fermentum 2kg, heart of a lotus seed benevolence bud juice 31.72kg, Pollen of Masson Pine extracting solution 59.08kg, 55 type high fructose syrup 3kg;
(5) fermented type gas water beverage is prepared:
Heart of a lotus seed benevolence bud juice and Pollen of Masson Pine extracting solution are added in fermentor tank, sterilizing, add beverage lactobacillus bulgaricus, beverage thermophilic bacteria and cereuisiae fermentum, in 37.5 DEG C of bottom fermentation 22.5h; Add Trisodium Citrate and 55 type high fructose syrups, pasteurization, obtains raw mixture; Add the chitosan of raw mixture quality 1% again as finings, to be precipitated completely after, filter, get filtrate, to obtain final product.
embodiment 4
A preparation method for fermented type gas water beverage, comprises the following steps:
(1) heart of a lotus seed benevolence bud juice is prepared:
Get black shell heart of a lotus seed benevolence and gold heart of a lotus seed benevolence, the mass ratio of black shell heart of a lotus seed benevolence and gold heart of a lotus seed benevolence is 1:3, at room temperature sprouts 2 day after soaking 23h in 30 DEG C of water; Get germination heart of a lotus seed benevolence and dry moisture content at 50 DEG C, be crushed to 90 orders, shell, obtain heart of a lotus seed benevolence bud powder; In heart of a lotus seed benevolence bud powder, add the water of heart of a lotus seed benevolence bud opaque amount 10% and the yeast of 1%, by the kneading of heart of a lotus seed benevolence bud powder to bulk, in 35 DEG C of bottom fermentation 10h, then toast 15min at 180 DEG C, be cooled to 42 DEG C, be crushed to 70 orders; Add water, the mass ratio of heart of a lotus seed benevolence bud powder and water is 1:1, obtains heart of a lotus seed benevolence bud powder solution; Add the saccharifying enzyme saccharification of heart of a lotus seed benevolence bud powder solution quality 3%, filter, get filtrate and obtain heart of a lotus seed benevolence bud juice;
(2) Pollen of Masson Pine extracting solution is prepared:
Get Pollen of Masson Pine, clear water embathes rear filtration, and filter cake is added clear water by the mass ratio of 1:22, in 50 DEG C, ultrasonication 60min under 80% power, then stored refrigerated at being placed in 2 DEG C, obtain Pollen of Masson Pine extracting solution;
(3) domesticated strain:
Extracting degreasing breast prepares nutrient solution with the mass ratio that 9:1 press by Pollen of Masson Pine extracting solution, using the lactobacillus bulgaricus that activated and thermophilic bacteria as bacterial classification, accesses in nutrient solution by the inoculum size of nutrient solution volume ratio each 2%; At postvaccinal nutrient solution is placed in 43 DEG C, after nutrient solution solidifies, to prepare in the nutrient solution obtained by 8:2 mass ratio to skimming milk and Pollen of Masson Pine extracting solution by cultivating the strain inoculation that obtains, at being still placed in 43 DEG C, after nutrient solution solidifies, by that analogy, continue to cultivate, until the mass ratio of skimming milk and Pollen of Masson Pine extracting solution reaches 1:1; Finally at 40 DEG C, cultivate bacterial classification 12.5h separately with Pollen of Masson Pine extracting solution, the volume ratio of bacterial classification and Pollen of Masson Pine extracting solution is 1:20, obtains beverage lactobacillus bulgaricus and thermophilic bacteria;
(4) drink formula is determined:
Each raw material is taken by following quality proportioning:
Trisodium Citrate 0.1kg, beverage lactobacillus bulgaricus 1kg, beverage thermophilic bacteria 1kg, cereuisiae fermentum 3kg, heart of a lotus seed benevolence bud juice 32kg, Pollen of Masson Pine extracting solution 60kg, 55 type high fructose syrup 2.9kg;
(5) fermented type gas water beverage is prepared:
Heart of a lotus seed benevolence bud juice and Pollen of Masson Pine extracting solution are added in fermentor tank, sterilizing, add beverage lactobacillus bulgaricus, beverage thermophilic bacteria and cereuisiae fermentum, in 36 DEG C of bottom fermentation 24h; Add Trisodium Citrate and 55 type high fructose syrups, pasteurization, obtains raw mixture; Add the chitosan of raw mixture quality 1% again as finings, to be precipitated completely after, filter, get filtrate, to obtain final product.
The above, it is only preferred embodiment of the present invention, not any pro forma restriction is done to the present invention, anyly do not depart from technical solution of the present invention content, the any simple modification done above embodiment according to technical spirit of the present invention, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (4)
1. a fermented type gas water beverage, in obtained beverage for 100%, the mass percent of each component that this beverage packets contains is:
Trisodium Citrate 0.1-0.3%;
Lactobacillus bulgaricus 1-3%;
Thermophilic bacteria 1-3%;
Cereuisiae fermentum 1-3%;
Heart of a lotus seed benevolence bud juice 31-32%;
Pollen of Masson Pine extracting solution 59-60%;
55 type high fructose syrup 2-4%.
2. a kind of fermented type gas water beverage as claimed in claim 1, in obtained beverage for 100%, the mass percent of each component that this beverage packets contains is:
Trisodium Citrate 0.2%;
Lactobacillus bulgaricus 2%;
Thermophilic bacteria 2%;
Cereuisiae fermentum 2%;
Heart of a lotus seed benevolence bud juice 3l.72%;
Pollen of Masson Pine extracting solution 59.08%;
55 type high fructose syrups 3%.
3. a kind of fermented type gas water beverage as claimed in claim 1 or 2, wherein: described heart of a lotus seed benevolence bud juice is prepared by the mass ratio of 1:3 by black shell heart of a lotus seed benevolence and gold heart of a lotus seed benevolence.
4. a preparation method for fermented type gas water beverage, comprises the following steps:
(1) heart of a lotus seed benevolence bud juice is prepared:
Get after heart of a lotus seed benevolence soaks 20-25h in 25-35 DEG C of water and at room temperature sprout 2-3 days; Get germination heart of a lotus seed benevolence and dry moisture content at 45-55 DEG C, be crushed to 80-120 order, shell, obtain heart of a lotus seed benevolence bud powder; Add water, the mass ratio of heart of a lotus seed benevolence bud powder and water is 1:1, obtains heart of a lotus seed benevolence bud powder solution; Add the saccharifying enzyme saccharification of heart of a lotus seed benevolence bud powder solution quality 2-4%, filter, get filtrate and obtain heart of a lotus seed benevolence bud juice;
(2) Pollen of Masson Pine extracting solution is prepared:
Get Pollen of Masson Pine, clear water embathes rear filtration, and filter cake is added clear water by the mass ratio of 1:20-25, in 45-55 DEG C, ultrasonication 55-65min under 75-85% power, then stored refrigerated at being placed in 0-4 DEG C, obtain Pollen of Masson Pine extracting solution;
(3) domesticated strain:
Extracting degreasing breast prepares nutrient solution with the mass ratio that 9:1 press by Pollen of Masson Pine extracting solution, using the lactobacillus bulgaricus that activated and thermophilic bacteria as bacterial classification, accesses in nutrient solution by the inoculum size of nutrient solution volume ratio each 2%; At postvaccinal nutrient solution is placed in 40-45 DEG C, after nutrient solution solidifies, to prepare in the nutrient solution obtained by 8:2 mass ratio to skimming milk and Pollen of Masson Pine extracting solution by cultivating the strain inoculation that obtains, at being still placed in 40-45 DEG C, after nutrient solution solidifies, by that analogy, continue to cultivate, until the mass ratio of skimming milk and Pollen of Masson Pine extracting solution reaches 1:1; Finally at 37-42 DEG C, cultivate bacterial classification 11-13h separately with Pollen of Masson Pine extracting solution, the volume ratio of bacterial classification and Pollen of Masson Pine extracting solution is 1:20, obtains beverage lactobacillus bulgaricus and thermophilic bacteria;
(4) drink formula is determined:
Each raw material is taken by following quality proportioning:
Trisodium Citrate 0.1-0.3%, beverage lactobacillus bulgaricus 1-3%, beverage thermophilic bacteria 1-3%, cereuisiae fermentum 1-3%, heart of a lotus seed benevolence bud juice 31-32%, Pollen of Masson Pine extracting solution 59-60%, 55 type high fructose syrup 2-4%;
(5) fermented type gas water beverage is prepared:
Heart of a lotus seed benevolence bud juice and Pollen of Masson Pine extracting solution are added in fermentor tank, sterilizing, add beverage lactobacillus bulgaricus, beverage thermophilic bacteria and cereuisiae fermentum, in 35-40 DEG C of bottom fermentation 20-25h; Add Trisodium Citrate and 55 type high fructose syrups, pasteurization, obtains raw mixture; Add the chitosan of raw mixture quality 1% again as finings, to be precipitated completely after, filter, get filtrate, to obtain final product.
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