CN101322472B - Method for transferring male sterile line of Chinese pink and cultivating propagating seeds and hybrid seeds - Google Patents
Method for transferring male sterile line of Chinese pink and cultivating propagating seeds and hybrid seeds Download PDFInfo
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- CN101322472B CN101322472B CN2008100208610A CN200810020861A CN101322472B CN 101322472 B CN101322472 B CN 101322472B CN 2008100208610 A CN2008100208610 A CN 2008100208610A CN 200810020861 A CN200810020861 A CN 200810020861A CN 101322472 B CN101322472 B CN 101322472B
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Abstract
The invention relates to a pink male sterile line transformation and a cultivation method for the breed and coenospecies, belonging to the technical field of plant cultivation. The method uses the existing pink male sterile line as a female parent and at least two pink self compatible lines as male parents, and biparental cross is carried out respectively with the pink male sterile line as the female parent; a single plant mostly close to the economic character of the male parent is selected from the later generations of the biparental cross as the female parent and then backcrossed with the original male parent; the backcrossing is repeated, and after at least five generations of backcrossing and transformation, the pink male transformation sterile line is obtained. The pink male transformation sterile line obtained by adopting the method of the invention has the advantages of stable male sterility, better combining ability, and the like; by means of multiple self-crosses and line-crosses, most combinations show the heterobeltiosis of disease resistance, growing luxuriance, flowering branch increasing, colored flower, and the like; the labor and cost can be obviously saved.
Description
Technical field
The present invention relates to the cultivating method of breeding of a kind of China pink, especially a kind of China pink male sterile line transformation and breeding kind and the crossbreed cultivating method belongs to the domestication of plants technical field.
Background technology
China pink (Dianthus chinensis L.) is one of Chinese traditional famous flower, originates in Northeast China, North China, the Yangtze river basin and south east asia, and it is very wide to distribute.Because of its pattern is delicate and charming, the florescence is longer, and often make potted flower (flower bed) and cut-flower and use, be the important flower variety that beautifies urban and rural environment.
Crossbreeding is an important means creating Flower New Variety kind or newtype, can not only combine two or more kinds, the good characteristic and the feature of kind, can also produce huge hybrid vigour, improves the growth potential, resistance of flowers etc.According to document announcement, Italy, Holland, Poland, Israel, the U.S. etc. can only cultivate the China pink Hybrid by the conventional hybridization method of utilizing artificial emasculation at present, but this method is time-consuming.
Summary of the invention
The objective of the invention is to: at the shortcoming of above-mentioned prior art existence, a kind of transfer method of China pink male sterile line of saving of labor's abridged edition is proposed, and then be given in breeding kind and the crossbreed cultivating method of China pink male sterile line transformation on the method basis, thereby create good conditions for cultivating how better China pink flowers new varieties or newtype.
In order to reach above purpose, China pink male sterile line transfer method of the present invention may further comprise the steps:
The first step, being maternal with existing China pink male sterile, is male parent with at least two China pink self-compatibles, carries out biparental cross with China pink male sterile line as female parent respectively; Described male sterile is 20480A or 20468A, and described self-compatible is one of 20708B, 20710B, 20714B, 20715B, 20718B;
Second step, from the biparental cross offspring, select with the thorough individual plant of the immediate sterility of male parent economic characters be female parent, backcross with former male parent again;
The 3rd step, repeated for second step,, obtain the male transformation male sterile line of China pink by three generations's backcross transformation at least.
Can be preferable material voluntarily as the existing China pink male sterile line of female parent for the first time, also can be one of 20480A, 20468A; Self compatible line also can be one of 20708B, 20710B, 20712B, 20714B, 20715B, 20718B except that oneself is selected; The strain of above China pink standard code was succeeded in developing respectively at 2002, and public offering, can on market, buy (for example buying No. 112, Ningbo-Hangzhou road, Jurong City, Jiangsu Province, address) as required, so the present invention is practical to the Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute.
Compared with prior art, the ingenious employing transfer method of this method can obtain that sterility is very stable, the plant blossom fruit character is normal, the male transformation male sterile line of China pink that coordinate force is good, and simple, saving of labor's abridged edition.
On this basis, the cultivating method of breeding seed and hybrid seed of China pink male sterile line of the present invention transformation may further comprise the steps
Step 1, will be as the male transformation male sterile line of the China pink of female parent, lie in planted in fall as the maintenance line (self compatible line that promptly is used for continuous backcross) and the self-compatible of male parent;
Step 2, treat 6 leaves after, with China pink male transformation male sterile line plant and maintenance line plant, China pink male transformation male sterile line plant and self compatible line plant, respectively by row than 3-5: 1 transplants to the land for growing field crops that is separated from each other, preferably in two separation net canopies;
When step 3, the plant blossom after waiting to transplant, pollinate;
Step 4, treat seed maturity after, gather and be separated from each other the seed on (or two net canopy in) the male transformation male sterile line of China pink plant respectively, promptly obtain the China pink male sterile line and breed seed and required China pink male sterile line hybrid seed.
Experimental results show that the male transformation male sterile line of China pink that adopts the inventive method to obtain has following outstanding advantage: male sterility is stable, sterile plant rate and sterile strain degree are 100%, be not subjected to environmental influence, the crossbreed hybrid rate with its preparation in isolated area can reach 100%; Coordinate force is preferably arranged, heterobeltiosis such as with the hybridization of itself and a plurality of self compatible line, that most combination table reveal is disease-resistant, growth potential is vigorous, the spray number increases, pattern is gorgeous; Under the isolation condition male sterile line breed and the cultivation process of crossbreed in all available honeybee pollination, significantly saving of labor's abridged edition.
Description of drawings
The present invention is further illustrated below in conjunction with accompanying drawing.
Fig. 1 is the procedure schema figure of a transformation embodiment of the present invention.
Fig. 2 is the procedure schema figure of a hybridization of the present invention embodiment.
Embodiment
Embodiment one
Present embodiment is referring to Fig. 1, sterility is stable in order to obtain, vine growth and development and normal, the economic characters good stable of blossoming and bearing fruit, the male transformation male sterile line of China pink that coordinate force is strong, taked the transfer method of continuous backcross, make female parent with preferred existing China pink male sterile line 20480A or 20468A, and select the existing height that proterties is stable, coordinate force is strong to carry out biparental cross for male parent for one of self compatible line 20708B, 20710B, 20714B, 20715B, 20718B; And then selection is maternal with the thorough individual plant of the immediate sterility of male parent economic characters from the biparental cross offspring, backcrosses with former male parent again; So continuous backcross is many for (for example five generations), until obtaining the male transformation male sterile line of China pink that main economic characters are similar to the male parent of backcrossing.Usually with after 5 generations of fixing stable self compatible line transformation, just can obtain the economic characters China pink male transformation male sterile line similar with the male parent of backcrossing, and the male parent that is used for continuous backcross is exactly a maintenance line to coordinate force.The proterties quality of maintenance line directly influences the male transformation male sterile line of the China pink proterties after the transformation success, and good maintenance line should possess following properties: (1) principal character characteristic good, stable and consistent; (2) coordinate force is preferably arranged; (3) backcross transformation offspring male sterile plant rate and sterile degree all reach 100%; (4) florescence selfing performance is affine.The Other Main Agronomic Characters of present embodiment maintenance line is referring to table 1.
Table 1
| The maintenance line title | Plant height (cm) | Degree of development (cm) | The mode of blossoming | Scape size (cm) | Pattern | Field planting is to the fate of blooming (d) | Individual plant spray number (individual) | The florescence scope |
| 20708B? | 39? | 27.5× 28 | Poly-flower | 5～7? | Purplish red | ?75? | 15? | February-July |
| 20710B? | 51? | 34× 35.9 | Poly-flower | 6～8? | Dark red | ?80? | 18.2? | February-July |
| 20712B? | 53? | 36.4× 38.3 | Poly-flower | 8～9? | Rose-red | ?80? | 20.4? | February-July |
| 20714B? | 24? | 24.5× 26.7 | Only head | 3～5? | Pale red | ?70? | 80.3? | February-July |
| 20715B? | 23? | 23.8× 25.4 | Only head | 2～4? | Dark red | ?65? | 75.1? | January-July |
| 20718B? | 28? | 26.3× 27.8 | Only head | 3～5? | Purple | ?70? | 75.3? | February-July |
Annotate: B represents maintenance line
But present embodiment obtains the Other Main Agronomic Characters of the male transformation male sterile line of China pink of practical application and sees Table 2.
Table 2
| The cytoplasmic male sterile line title | Plant height (cm) | Degree of development (cm) | The mode of blossoming | Scape size (cm) | Pattern | Field planting is to the fate of blooming (d) | Individual plant spray number (individual) | Male sterile plant rate % | Male sterile degree % | The florescence scope |
| 20708A? | 39.5? | 28× 28.5 | Poly-flower | 5～7? | Purplish red | 75? | 15.8? | 100? | 100? | February-July |
| 20710A? | 51.5? | 35.5× 36 | Poly-flower | 6～8? | Dark red | 80? | 18.7? | 100? | 100? | February-July |
| 20712A? | 53.8? | 37.1× 38.7 | Poly-flower | 8～9? | Rose-red | 80? | 20.9? | 100? | 100? | February-July |
| 20714A? | 24.2? | 24.5× 26 | Only head | 3～5? | Pale red | 70? | 80.8? | 100? | 100? | February-July |
| 20715A? | 23.3? | 24.2× 26 | Only head | 2～4? | Dark red | 65? | 75.8? | 100? | 100? | January-July |
| 20718A? | 28.4? | 26.9× 28.4 | Only head | 3～5? | Purple | 70? | 75.7? | 100? | 100? | February-July |
Annotate: A represents male sterile line
On this basis, present embodiment further utilizes the China pink male sterile line to obtain it and breeds seed, the male transformation male-sterile seed of China pink that transformation is obtained as female parent and accordingly as the maintenance line seed of male parent in planted in fall; Treat to be transplanted in the net canopy in breeding land for growing field crops than 4: 1 by row China pink male transformation male sterile line plant and maintenance line plant respectively behind 6 leaves; Putting honeybee into when being with the plant blossom after transplanting pollinates; After treating seed maturity, be the seed of breeding of the male transformation male sterile line of China pink from the seed of the male transformation male sterile line of China pink plant results.
Embodiment two
The method that present embodiment obtains the male transformation male sterile line of China pink is identical with embodiment one, on this basis, present embodiment further utilizes the male transformation male sterile line of China pink to obtain its hybrid seed, referring to Fig. 2, at first will as the male transformation male sterile line of the China pink of female parent and as the self compatible line seed of crossbreed male parent system in planted in fall; Treat behind 6 leaves China pink male transformation male sterile line plant and self compatible line plant respectively by row be transplanted to the breeding land for growing field crops than 4: 1, preferably in the net canopy; Put honeybee when to be bloomed into and be used for pollination; After treating seed maturity, the seed of gathering in the crops from the male transformation male sterile line of China pink plant is the hybrid seed of preparation.It is strong that present embodiment makes hybrid combination growth potential, and the spray number is many, has tangible hybrid vigour, and its economic characters and male parent relatively see Table 3.
Table 3
Above-mentioned with the male transformation male sterile line cultivation of China pink China pink crossbreed, note following technical essential:
(1) begins sowing in good time and grow seedlings: generally sow in mid or late September.The field piece that Seedlings nursery should be selected higher, the easy row of physical features, easily pour water, 2-3 leaf thinning are after planting once transplanted behind 6 true leaves to the land for growing field crops, and growing period is wanted timely prevention and elimination of disease and pests.
(2) Shi Yi female parent and male parent ratio: physical features is smooth, the plot of the fertile irrigation and drainage in soil but seed farm should be selected.Generally under with the condition of density the row of maternal and male parent than being 4: 1.
(3) guarantee distance isolation distance and the honeybee source of pollinating: China pink is a cross pollinated plant, easy and dianthus crop hybrid, and seed farm and other dianthus crop isolation distance should be more than 1500 meters.China pink is an entomophilous flower, does maternal preparing hybrid kind with male transformation male sterile line, and every mu of seed farm is put honeybee 2-3 case at least, could guarantee that pollination fully.
(4) moment is noted parent's purity: each link in the production of hybrid seeds, comprise seedling stage, field growth period, flowering stage, and note parent's roguing.
(5) regulate parents' florescence unanimity, in time pull out the strain of male parent kind, gather in the crops seed in good time.
If father and mother are inconsistent this flowering stage, the measures such as date of seeding and training of should taking to stagger are regulated parents' florescence consistent as far as possible.After parents' florescence finishes, the strain of male parent kind all should be pulled out immediately, prevent to mix.When kind of English yellowish-brown, should in time gather in the crops the seed on the maternal row, the grenadine bag of packing in time dries the seed of laying, and preventing to go mouldy influences seed germination rate.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (1)
1. China pink male sterile line transfer method may further comprise the steps:
The first step, being maternal with existing China pink male sterile, is male parent with at least two China pink self-compatibles, carries out biparental cross with China pink male sterile line as female parent respectively; Described male sterile is 20480A or 20468A, and described self compatible line is selected from 20708B, 20714B, 20715B;
Second step, from the biparental cross offspring, select with the thorough individual plant of the immediate sterility of male parent economic characters be female parent, backcross with former male parent again;
The 3rd step, repeated for second step,, obtain the male transformation male sterile line of China pink by three generations's backcross transformation at least.
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| CN101322472B true CN101322472B (en) | 2011-04-20 |
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| CN103416295B (en) * | 2013-08-09 | 2016-03-02 | 镇江瑞繁农艺有限公司 | The selection of the short raw hollyhock cytoplasm male sterility line of a kind of polyphyll and breeding method |
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