CN101283658B - Ecological cultivation method for spire morel - Google Patents
Ecological cultivation method for spire morel Download PDFInfo
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- CN101283658B CN101283658B CN2008100584820A CN200810058482A CN101283658B CN 101283658 B CN101283658 B CN 101283658B CN 2008100584820 A CN2008100584820 A CN 2008100584820A CN 200810058482 A CN200810058482 A CN 200810058482A CN 101283658 B CN101283658 B CN 101283658B
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- morchellaconica
- bacterial classification
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Abstract
The invention provides an ecological cultivation method of morchella conica. The method comprises the steps of morchella conica strain preparation, round-leaved poplar culture, round-leaved poplar treatment before strain inoculation, morchella conica strain inoculation to round-leaved poplar, and transplanting and managing after morchella conica strain inoculation. The invention is characterized in that 10-80% of the root part of round-leaved poplar is mechanically damaged and sterilized and then inoculated with morchella conica strain, followed by cultivation for 10-120 days. The method in the invention has the advantages that: (1) morchella conica grows under natural conditions and retain original property; (2) the yield of morchella conica is greatly increased by 20 times as compared with the yield under natural wild conditions; and (3) the forest coverage rate is efficiently increased by planting round-leaved poplar. The method is beneficial for ecological environment protection and sustainable development of morchella conica culture.
Description
Technical field
The present invention relates to a kind of ecological cultivation method for spire morel.
Background technology
Morchellaconica (Morchella conica) is commonly called as sheep sparrow bacterium, maize bacterium, pockmarks bacterium, wolf tripe.Belong to Ascomycotina (Aseomycotina), discomycete (Discomycetes), Pezizale (Pezizales), Morchellaceae (Morchellaceae), morchella (Morchella).Morchellaconica is rich in amino acid and vitamin, and meat is tender and crisp, and unique flavor is one of treasure in edible and the medicinal fungus.In recent years, wild toadstool is because of being subjected to such environmental effects such as weather, height above sea level, temperature, humidity, soil, and output is successively decreased year by year, and price also rises steadily.Therefore; domestic and international numerous scientific workers have carried out the research of hickory chick artificial cultivation; priorities such as (2000) such as (nineteen ninety-fives) such as Liu Bo (nineteen fifty-three), Chen Huiqun, Dong Shufeng (nineteen ninety-five), Li Suling has been delivered the report of hickory chick artificial cultivation success; but these method poor repeatability are not also accomplished scale production.American Ower and Miller are respectively at applying for United States Patent (USP) (patent No. of Ower is respectively 4594809 and 4866878, patent No. us204000090A and the us2040060230A of Miller) in 1988 and 2004; its patent adopts double-deck medium such as wheat; induce the generation fruit body again after turning out sclerotium; but there are problems such as cost height, complex procedures, planting environment requirement harshness, fail large-scale planting so far.Zhu Douxi, Kumin bacteria Court Food Co., Ltd and Chen Wanpeng etc. have applied for Chinese patent, the number of patent application of Zhu Douxi application is respectively 93115364.6 and 00112812.4, the number of patent application of Kumin bacteria Court Food Co., Ltd's application is 20610010962.0, and the number of patent application of Chen Wanpeng application is 20710185274.2.But the patent employing organic matter of Zhu Douxi, Chen Wanpeng application etc. is made medium, and there is the cost height equally in the similar conventional edible mushroom of other technologies, and technological process is difficult to common peasant household to be accepted, thereby does not also see the large tracts of land popularization; The patent of Kumin bacteria Court Food Co., Ltd application is unfavorable for forest conservation owing to need to take clearly measure such as woods in the course of cultivation.
Summary of the invention
The objective of the invention is to remedy the deficiency of above-mentioned hickory chick artificial cultivation technique, provide a kind of and can keep the original natural quality of hickory chick and improve output, can promote the ecological cultivation method for spire morel of planting trees again.
The present invention realizes like this, comprise that the Morchellaconica bacterial classification is prepared, the roundleaf poplar is cultivated, the roundleaf poplar inoculates the bacterial classification pre-treatment, the Morchellaconica bacterial classification inoculation transplants and manage and protect step after roundleaf poplar, roundleaf poplar are connect the Morchellaconica bacterial classification, after it is characterized in that roundleaf poplar root carries out mechanical damage 10-80% and sterilization, move in the Morchellaconica bacterial classification and cultivated 10-120 days.
Technical scheme of the present invention is:
1, the Morchellaconica bacterial classification is prepared: adopting conventional edible fungus species making, breeding method making Morchellaconica bacterial classification, can be liquid spawn, also can be solid spawn.
2, roundleaf poplar seedling culture: adopt conventional plant seminal propagation or cuttage breeding method to cultivate roundleaf poplar seedling, roundleaf poplar inoculation vaccine is advisable with the no damage by disease and insect roundleaf poplar seedling of plant height 20-200cm, straight stem 2-15cm.
3, thimerosal is prepared: adopt the routine disinfection agent, as the 0.1-1000ppm thimerosal of configurations such as carbendazim, potassium permanganate.
4, roundleaf poplar root mechanical damage is handled: will wait to inoculate roundleaf poplar children shoot root and cut off the part fibrous root, and carry out the injured processing of artificial mechanism to remaining, with injured being advisable of 10-80% of residue root system.
5, sterilization: will deploy in sterilization pool through the roundleaf poplar shoot root that the root mechanical damage is handled, the thimerosal in the sterilization pool was advisable to soak all root systems of roundleaf Yang Miao, sterilized 3-180 minute.
6, inoculation: the roundleaf Yang Miao after will sterilizing places the Morchellaconica bacterial classification through clear water rinsing back root part, wherein liquid spawn was advisable to soak all root systems of roundleaf Yang Miao, solid spawn covers around the root system then be divided into the fritter of diameter 0.5-3cm before inoculation after again, cultivates 8-90 days under the natural conditions.
7, the roundleaf poplar is transplanted after being connect the Morchellaconica bacterial classification: roundleaf Yang Miao is transplanted in the forest land after being connect the Morchellaconica bacterial classification, backfill soil is during to the dark 1/2-2/3 in the pool, around distance seedling 0.5-30cm, add 1-10g ash, 1-5g Portland cement, 5-1000g solid spawn; It is flat to the pool to continue backfill soil.
8, manage and protect: the field planting of roundleaf poplar used the Morchellaconica liquid spawn to root teeming 1-4 time in three months, and weeding 1-2 time keeps soil moisture and prevents weeds propagation and widespread; Shade and spray water carrying out the forest land next year 1-6 month,, promote the Morchellaconica fruit body to take place to keep forest land humidity and moisture.
Use the present invention, the one, hickory chick grows under field conditions (factors), has kept original natural quality; The 2nd, hickory chick output significantly improves, and improves more than 20 times than the output of wild condition; The 3rd, by plantation roundleaf poplar, can effectively improve forest coverage, promote ecological environmental protection and hickory chick sustainable development.
Embodiment
Embodiment 1:
1, the roundleaf poplar is prepared: get 5 months no damage by disease and insect seedling of roundleaf poplar cottage propagation,
2, the Morchellaconica bacterial classification is prepared: press glucose 20g, peptone 4g, MgSO
40.05g, KH
2PO
40.05g, K
2HPO
40.05g, H
2The ratio preparation bacterium culture medium of O 1000ml.Medium was sterilized 30 minutes under 121 ℃ of conditions, and cooling back inserts that Morchellaconica is female plants, 20 ℃ of condition bottom fermentations cultivate 30 days standby.
3, roundleaf Yang Miao root mechanical damage and sterilization: the roundleaf poplar is cut off fibrous root, and carry out artificial mechanism damage to remaining, damaged area is about 50%, and after roundleaf poplar root put into the 50ppm liquor potassic permanganate and soak 5 minutes, with clear water rinsing 2 times.
4, inoculation: will soak with the Morchellaconica liquid spawn through root mechanical damage and the roundleaf of disinfecting poplar seedling root, be advisable to cover injured whole root system, long root system can excise, and cultivates 30 days.
5, transplanting: be split into the ribbon level land in the also forest land of preparing plantation along plantation plot contour, band center distance (line-spacing) 1.5m digs the roundleaf poplar of 40 * 40 * 40cm and plants the pool by spacing in the rows 200cm; Seedling is stood on pool central authorities, a hand steered straight seedling, the proficiency backfill soil, the soil of backfill is wanted no-sundries in small, broken bits; Backfill soil adds 5g ash during to the dark 1/2-2/3 in the pool around distance seedling 5cm, the 5g Portland cement, and the 10g humus, the 500g bacterial classification continues backfill soil to the pool at ordinary times.
6, manage and protect: in the roundleaf poplar field planting three months with Morchellaconica bacterial classification liquid to root teeming 2 times and weeding 1 time, keep soil moisture and prevent weeds propagation and widespread.Shade and spray water carrying out the forest land next year 1-6 month respectively afterwards,, promote the Morchellaconica fruit body to take place to keep forest land humidity and moisture
Embodiment 2:
1, the roundleaf poplar is prepared: getting 2 months roundleaf poplars of cottage propagation does not have the damage by disease and insect seedling,
2, the Morchellaconica microbial inoculum is prepared: fermentation medium is the PDA medium, and medium sterilize 30 minutes under 121 ℃ of conditions, and the cooling back inserts that Morchellaconica is female plants, and is standby 25 ℃ of condition bottom fermentations cultivations 20 days.
3, roundleaf poplar root mechanical treatment and sterilization: the roundleaf poplar is cut off fibrous root, and carry out artificial mechanism damage to remaining, damaged area is about 30%, and after roundleaf poplar root put into the 10ppm liquor potassic permanganate and soak 10 minutes, with clear water rinsing 3 times.
4, inoculation: will soak with the Morchellaconica liquid spawn through root mechanical damage and the roundleaf of disinfecting poplar seedling root, be advisable to cover injured whole root system, long root system can excise; Cultivated 30 days.
5, transplant: be split into the ribbon level land in the forest land of preparing plantation along plantation plot contour, band center distance (line-spacing) 2m digs the roundleaf poplar of 40 * 40 * 40cm and plants the pool by spacing in the rows 200cm; Seedling is stood on pool central authorities, a hand steered straight seedling, the proficiency backfill soil, the soil of backfill is wanted no-sundries in small, broken bits; Backfill soil adds 5g ash, 5g Portland cement, 10g humus, 600g bacterial classification during to the dark 1/2-2/3 in the pool around distance seedling 5cm; Continue backfill soil at ordinary times to the pool.
6, manage and protect: the method for managing and protecting is with example 1.
Embodiment 3:
1, the roundleaf poplar is prepared: get 10 months no damage by disease and insect seedling of roundleaf poplar seminal propagation,
2, the Morchellaconica microbial inoculum is prepared: fermentation medium is the PDA medium, and medium sterilize 30 minutes under 121 ℃ of conditions, and the cooling back inserts that Morchellaconica is female plants, and is standby 25 ℃ of condition bottom fermentations cultivations 30 days.
3, roundleaf poplar root mechanical treatment and sterilization: the roundleaf poplar is cut off fibrous root, and carry out artificial mechanism damage to remaining, injured area is about 70%, and after roundleaf poplar root put into the 60ppm liquor potassic permanganate and soak 3 minutes, with clear water rinsing 3 times.
4, inoculation: will be divided into before inoculation with solid spawn through root mechanical damage and the roundleaf of disinfecting poplar seedling root and cover behind the fritter of diameter 0.5-3cm around the root system again, cultivation is 70 days under the natural conditions.
5, transplant: the conceding the land to forestry ground in the preparation plantation is split into the ribbon level land along plantation plot contour, and band center distance (line-spacing) 1.8m digs the roundleaf poplar of 30 * 30 * 30cm and plants the pool by spacing in the rows 150cm; Seedling is stood on pool central authorities, a hand steered straight seedling, the proficiency backfill soil, the soil of backfill is wanted no-sundries in small, broken bits; Backfill soil adds 5g ash during to the dark 1/2-2/3 in the pool around distance seedling 5cm, the 5g Portland cement, and the 8g humus, the 400g bacterial classification continues backfill soil to the pool at ordinary times.
6, manage and protect: the method for managing and protecting is with example 1.
Claims (6)
1. ecological cultivation method for spire morel, comprise that the Morchellaconica bacterial classification is prepared, roundleaf Yang Miao cultivates, roundleaf Yang Miao inoculates the bacterial classification pre-treatment, the Morchellaconica bacterial classification inoculation transplants and manage and protect step after roundleaf Yang Miao, roundleaf Yang Miao are connect the Morchellaconica bacterial classification, after it is characterized in that roundleaf Yang Miao root carries out mechanical damage 10-80% and sterilization, move in the Morchellaconica bacterial classification and cultivated 10-120 days; Roundleaf Yang Miao is transplanted in the forest land after being connect the Morchellaconica bacterial classification, and backfill soil adds 1-10g ash during to the dark 1/2-2/3 in the pool around distance seedling 0.5-30cm, the 1-5g Portland cement, and the 5-1000g solid spawn, it is flat to the pool to continue backfill soil; In the roundleaf Yang Miao field planting three months with the Morchellaconica liquid spawn to root teeming 1-4 time, weeding 1-2 time is shaded and is sprayed water carrying out the forest land next year 1-6 month.
2. ecological cultivation method for spire morel according to claim 1 is characterized in that the Morchellaconica bacterial classification is a liquid spawn.
3. ecological cultivation method for spire morel according to claim 1 is characterized in that the Morchellaconica bacterial classification is a solid spawn.
4. ecological cultivation method for spire morel according to claim 1, the roundleaf Yang Miao that it is characterized in that inoculating usefulness are the no damage by disease and insect roundleaf poplar seedling of plant height 20-200cm, straight stem 2-15cm.
5. according to claim 1 or 3 described ecological cultivation method for spire morel, it is characterized in that the Morchellaconica bacterial classification inoculation when roundleaf Yang Miao, solid spawn covers around the roundleaf poplar root system be divided into the fritter of diameter 0.5-3cm before inoculation after again.
6. ecological cultivation method for spire morel according to claim 1 and 2 is characterized in that the Morchellaconica bacterial classification inoculation when roundleaf Yang Miao, and liquid spawn was soaked all root systems of roundleaf Yang Miao.
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| CN2008100584820A CN101283658B (en) | 2008-06-02 | 2008-06-02 | Ecological cultivation method for spire morel |
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| CN2008100584820A CN101283658B (en) | 2008-06-02 | 2008-06-02 | Ecological cultivation method for spire morel |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946634A (en) * | 2010-09-13 | 2011-01-19 | 云南省农业科学院高山经济植物研究所 | Manufacturing method for morchella mother culture |
| CN103168622B (en) * | 2013-04-03 | 2015-02-25 | 中国科学院昆明植物研究所 | Cultivating method of morchella importuna |
| CN103907471B (en) * | 2014-04-24 | 2015-06-10 | 绵阳市沁禾农业科技有限公司 | Method for interplanting toadstools with radix ophiopogonis without cover |
| CN104206171A (en) * | 2014-08-28 | 2014-12-17 | 达州市农业科学研究所 | Activity keeping agent and application and preparation method thereof and wild propagation method for wild edible mushrooms |
| CN104838882A (en) * | 2015-04-22 | 2015-08-19 | 吴中区胥口精益生物医药研究所 | Bionic cultivating method for morchella conica |
| CN105613042B (en) * | 2016-01-25 | 2018-12-25 | 四川保兴现代农业科技股份有限公司 | A kind of the liquid spawn mating system and industrial planting method of hickory chick |
| CN110476711B (en) * | 2019-10-08 | 2022-05-20 | 丽江中源绿色食品有限公司 | Morchella cultivation method |
| CN112400607A (en) * | 2020-10-14 | 2021-02-26 | 安徽乐永园农业科技有限公司 | Preparation and use methods of culture medium suitable for culturing morchella |
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