CN101263766B

CN101263766B - Edible snakegourd root and bud in vitro vegetative propagation technique

Info

Publication number: CN101263766B
Application number: CN2008100607502A
Authority: CN
Inventors: 陈华夫
Original assignee: Individual
Current assignee: Individual
Priority date: 2008-04-20
Filing date: 2008-04-20
Publication date: 2011-08-03
Anticipated expiration: 2028-04-20
Also published as: CN101263766A

Abstract

The invention relates to an in vitro clonal propagation technique for edible snake gourd root buds, which is characterized in that, edible snake gourd has strong characteristic of root budding; in early spring, a shed is arranged; carefully selected mother roots with good quality, high yield and resistance are sterilized and then separately planted; temperature is maintained between 10 DEG C to 40 DEG D; relative humidity is above 85%; the higher the temperature, the faster the germination; a mulch film is covered on trench surface, and a plurality of plant spacing holes are arranged; when the length of the root bud seedlings reaches 7cm to 10cm, the root bud seedlings are planted in the plant spacing holes; temperature ranges from 20 DEG C to 30 DEG C, and relative humidity is above 90%; after 25 days to 35 days, rooting takes place. The in vitro clonal propagation technique for edible snake gourd root buds has the advantages that, the bud seedlings of the invention is formed by the vegetative mass of female mother roots; good quality, high yield, resistance and other fine traits of the product can be maintained without variation; the invention has the characteristics of simple operation, high reproductive rate, low cost, good antibacterial property and other characteristics.

Description

Edible snakegourd root and bud in vitro vegetative propagation technique

Technical field

The present invention relates to a kind of plant propagation technology, particularly a kind of edible snakegourd root and bud in vitro vegetative propagation technique.

Background technology

Hang melon and have another name called snakegourd, belong to Curcurbitaceae, perennial perennial root herbaceous species plant has medicinal and edible branch.Medicinal hang melon have be good for the stomach, moistening lung, reduce phlegm, lubricated effect, cure mainly phlegm-heat cough, lung productive cough blood, uncomfortable in chest, constipation etc.The melon seeds of edible melon has anti-inflammatory, anticancer effect, and is evident in efficacy to lung cancer, cancer of the stomach, insulin breast cancer, contains elements such as protein, fat, cellulose, trace element and alkaloid, flavonoids, edible good for health for a long time.

Edible melon is continued to use seminal propagation always, owing to hang the genetic cause of melon, complicated segregation phenomenon can appear in amphigenetic seedling offspring, and variation type is many, and quality discrepancy is big, and antibiotic property is poor, the output shakiness.Have only female about 40% among the offspring with seminal propagation, the 60%th, male, cause the female ratio imbalance, reproduction rate descends.What also have gives birth to the breeding of piece undercut section with 2-3, owing to be subjected to the quantitative limitation of piece radical, reproductive efficiency is low, and when taking a lot of work.The patent No. is 2006101614701 Chinese patent, discloses a kind of tissue culture seedling growing process for trichosanthes, comprises following step: 1, to hang bud or the rattan shape stem sprouted on the melon piece root be explant at the end of spring and the beginning of summer getting; 2, clip bud or rattan shape stem are removed leaf and tendril on the rattan shape stem, are cut into a base of leaf section, wait to inoculate; 3, with step 2 bud or stem section are seeded in the blake bottle I that medium I is housed bud of every bottle graft kind or a stem section; 4, step 3 is got stem with leaves and be cut into a base of leaf section again, be seeded in the blake bottle II that medium ii is housed, insert single stem section 4-6 for every bottle, 5, step 4 is got stem with leaves and be cut into a base of leaf section, be seeded in the blake bottle III that medium ii I is housed, insert 10 stem sections for every bottle, cultivated 20-30 days, can take root.This invention breeding cycle is short, reproduction coefficient height, but complex operation, and the culture fluid modulation is difficult to be grasped, and technology is promoted restricted.

Summary of the invention

The objective of the invention is to design a kind of simple to operate, reproduction rate height, the edible snakegourd root and bud in vitro vegetative propagation technique that cost is low, anti-microbial property is good.Technical scheme of the present invention is so to realize:

Edible snakegourd root and bud in vitro vegetative propagation technique comprises following step:

1) puts up a shed: season in the early spring, the bedding of whole ground, furrow face width 1-1.2m, furrow width 0.3-0.5m wholely, dig the dark heatable adobe sleeping platform of 13-16cm at the furrow face, the wood chip excrement sugar of basic upper berth 10-13cm is covered with the hay ash on it, repave the thick fine earth of one deck 4-6cm, having peace of mind, furrow face sterilization back is scaffolding in time;

2) seedbed soil preparation: the native 30-40% that selects anosis worm source, fine sand 30-35%, well-rotted farmyard manure 30-40% sprays bed soil with 0.8-1.2% potassium permanganate and 85-90% chlorophos crystalloid fluid behind the mixing, soil location humidity 75-83%, it is stand-by to take off film with the plastic foil sealing after 8-12 days;

3) select kind of a root, return Pu vernalization: select virus-free carry the kind root that no germ infects and carry out disinfection after, its spacing by 14-16cm is sequentially arranged in the seedbed, the seedbed soil that has prepared is poured between kind of root, and exceed kind of a root growth point 3-5cm, press slightly with hand, build on furrow that little shed is warming preserves moisture again, temperature keeps 10-40 ℃, relative moisture is early stage more than 85%, mid-term 7-10%, printing opacity carries out vernalization on time, during the long 7-10cm of bud bud is broken away from from kind of root, and it is stand-by to sterilize;

4) seedbed management: on the furrow face, spread mulch film earlier before the radical bud seed is planted, and the spacing in the rows of 6-8cm is punched on mulch film, with the radical bud seed of dipping in root-inducing powder in the hole, then with sandy soil with on the mulch film port lid, and build in time that little shed is warming preserves moisture, with the sunshade of sunshade net, temperature keeps 20-30 ℃, and relative moisture is more than 90%, make and be vaporific in the film, took root in 25-35 days, the back every one week spraying fungicide and foliage fertilizer 1-2 time, and impose root-inducing powder.

As preferably, described furrow face and radical bud are used 500-700 times of potassium permanganate sterilization.

As preferably, the shoot root of planting the radical bud in the ground fenestra in the described seedbed management is exposed at more than the mulch film.

The present invention has very strong ratoon characteristics according to edible melon, set up scaffolding in the early spring, with selected good quality and high output resistance kind root sterilization back isolation implant, temperature keeps 10-40 ℃, and relative moisture is more than 85%, temperature high germination more is fast more, spreading and beat the spacing in the rows hole on the furrow face, during radical bud seedling 7-10cm with in the radical bud seedling implantation hole, temperature 20-30 ℃, relative moisture is taken root after 25-35 days more than 90%.Bud seedling of the present invention is made up of the trophosome of female kind of root, can guarantee that the merit such as high-quality, high yield, resistance of product does not make a variation, have simple to operate, reproduction rate height, characteristics such as cost is low, anti-microbial property is good.

Embodiment

Below by embodiment, technical scheme of the present invention is described in further detail.

Embodiment: edible snakegourd root and bud in vitro vegetative propagation technique comprises following step:

1) puts up a shed: have very strong ratoon characteristics according to edible melon, in season in the early spring, select hidding and sunny side wholely, the illumination abundance, subterranean water level is difficult for too high, the plot that the fertile pine of soil property is dredged and gutless mistake is hung melon, the bedding of whole ground, furrow face width 1.1m, furrow width 0.4m, dig the dark heatable adobe sleeping platform of 15cm at the furrow face, the wood chip excrement sugar of base upper berth 12cm is covered with the hay ash on it, repave the thick fine earth of one deck 5cm, having peace of mind, the furrow face is in time put up a shed with 600 times of potassium permanganate sterilization backs;

2) seedbed soil preparation: select anosis worm source soil, fine sand, well-rotted farmyard manure each 1/3rd, spray bed soil with 1% potassium permanganate and 90% chlorophos crystalloid fluid behind the mixing, native location humidity 80%, it is stand-by to take off film after sealing 10 days with plastic foil;

3) select kind of a root, return Pu vernalization: select virus-free carry the kind root that no germ infects and carry out disinfection after, its spacing by 15cm is sequentially arranged in the seedbed of watering vola water, upright baffle around the seedbed, the seedbed soil that has prepared is poured between kind of root, and exceed kind of a root growth point 4cm, press slightly with hand, build on furrow again that little shed is warming preserves moisture, the initial stage temperature keeps 30 ℃, and relative moisture is early stage more than 85%, and be relative moisture 8% in vegetative period mid-term, printing opacity on time, carry out vernalization, during the long 7-10cm of bud bud is broken away from from kind of root, and sterilize stand-by with 550 times of potassium permanganate;

4) seedbed management: on the furrow face, spread mulch film earlier before the radical bud seed is planted, and the spacing in the rows of 7cm is punched on mulch film, to dip in the radical bud seed of root-inducing powder in the hole, the degree of depth is that shoot root is exposed at more than the mulch film, is preferably in cloudy day or dusk during kind, notes lucifuge, then with sandy soil with on the mulch film port lid, and build in time that little shed is warming preserves moisture, with the sunshade of sunshade net, plant back radical bud seedling and will keep fresh state, temperature keeps about 25 ℃, relative moisture makes to be vaporific in the film more than 90%, takes root in 30 days, the back every one week spraying fungicide and foliage fertilizer 1-2 time, and impose root-inducing powder, and in time in the thick fine earth of mulch film upper berth 2cm, the growth of weeds always.

Claims

1. edible snakegourd root and bud in vitro vegetative propagation technique is characterized in that comprising following step:

2. edible snakegourd root and bud in vitro vegetative propagation technique according to claim 1 is characterized in that 500-700 times of potassium permanganate sterilization of described furrow face and radical bud use.

3. edible snakegourd root and bud in vitro vegetative propagation technique according to claim 1 is characterized in that the shoot root of planting the radical bud in the ground fenestra in the described seedbed management is exposed at more than the mulch film.