CN101193906A - 细菌宿主细胞中甘露糖醇诱导的启动子系统 - Google Patents
细菌宿主细胞中甘露糖醇诱导的启动子系统 Download PDFInfo
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- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
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Abstract
Description
5’-TTGTCACAACCCCGTTTGAAGGCTGTAAT-3’ | SEQ.ID No.1 |
5’-TTGTCACCGCCGTTTTTGAAGGCTGTAAT-3’ | SEQ.ID No.2 |
5’-TTGTCAGCCCTGCGTCAGAAGGCTGTAAT-3’ | SEQ.ID No.3 |
5’-TTGTCGGTTGCGTGACGCGCCTGTGTAA-3’ | SEQ.ID No.4 |
5’-ACGAGTGCAAAAAAGTATCAGTAAGCGTGCTCCCAAGGAT-3’ | SEQ.ID.No.5 |
5’-ACGAGTGCAAAAAAGTATCAGTCCAAGTGCTCCCAAGGAT-3’ | SEQ.ID.No.6 |
5’-CCGAGTGCAAAAAAGTATCGATTCAAGTGCTAGGGATGAT-3’ | SEQ.ID.No.7 |
5’-GGCGGTGCAAAAAAGTATCGGTCGAAGTGCAGTCGAGGCT-3’ | SEQ.ID.No.8 |
GAGCGTGGGAACGATCAAGTGTTAAACACTGCACTGAGGATCGTTCCCGCGCTCCGCGTGGGCATGCATACCGTGACGCTCTGCGTCACCTGGGGACGCAGAGCGTCCCTAGCGGCGTTACCACGCGGAGCGTGGGAACGATCAGGTGGTCGACGAGTGCAAAAAAGTATCAGTAAGCGTGCTCCCAAGGATTTGTCACCGCCGTTTTTGAAGGCTGTAATCAACGCACACTCTTCCTGACTCCTTGTAGGAAGACACAACAACAATAACCGTCCTTCTGTAGCCCTCTGGGCGCGGAA | SEQ.ID.No.9 |
TGAGCAGGAAAATCTGTACGGTTTCGCGCCCTTCGCCATGCTGAAACGCCCTTCCCTGCGGTTATCGCGCCAATCCCGAGTGCAAAAAAGTATCGATTCAAGTGCTAGGGATGATTTGTCAGCCCTGCGTCAGAAGGCTGTAATCAGTGCACATTCTTCCCCCGCCGGAAGAACACAAAAACAATAACTGTCCTTCTGCCCCC | SEQ.ID.No.10 |
TAAGCGTGCTCCCAAGGATTTGTCACCGCCGTTTTTGAAGGCTGTAATCAACGCACACTCTTCCTGACTCCTTGTAGGAAGACACAACAACAATAACCGTCCTTCTGTAGCCCTCTGGGCGCGGAA | SEQ.ID.No.11 |
ACGAGTGCAAAAAAGTATCAGTAAGCGTGCTCCCAAGGATTTGTCACCGCCGTTTTTGAAGGCTGTAATCAACGCACACTCTTCCTGACTCCTTGTAGGAAGACACAACAACAATAACCGTCCTTCTGTAGCCCTCTGGGCGCGGAA | SEQ.ID.No.12 |
ACGAGTGCAAAAAAGTATCAGTAAGCGTGCTCCCAAGGATTTGTCACCGCCGTTTTTGAAGGCTGTAATCAACGCAC | SEQ.ID.No.13 |
科I.假单胞菌科 | 葡糖杆菌属(Gluconobacter)假单胞菌属(Pseudomonas)黄单胞菌属(Xanthomonas)动胶菌属(Zoogloea) |
科II.固氮菌科 | 氮单胞菌属(Azomonas)固氮菌属(Azotobacter)拜叶林克氏菌属(Beijerinckia)德克斯菌属(Derxia) |
科III.根瘤菌科 | 土壤杆菌属(Agrobacterium)根瘤菌属(Rhizobium) |
科IV.甲基单胞菌科 | 甲基球菌(Methylococcus)甲基单胞菌属(Methylomonas) |
科V.盐杆菌科 | 盐杆菌属(Halobacterium)盐球菌属(Halococcus) |
其它属 | 醋杆菌属(Acetobacter)产碱菌属(Alcaligenes)包特氏菌属(Bordetella)布氏杆菌属(Brucella)弗朗西斯氏菌属(Francisella)栖热菌属(Thermus) |
复制子 | 载体 |
pPS10 | pCN39,pCN51 |
RSF1010 | pKT261-3 |
pMMB66EH | |
pEB8 | |
pPLGN1 | |
pMYC1050 | |
RK2/RP1 | pRK415 |
pJB653 | |
pRO1600 | pUCP |
pBSP |
反应混合物(100μL总体积) | 热循环步骤 | |||||
10μL | 10X Herculase缓冲液* | 步骤1 | 1个循环 | 2分钟 | 94℃ | |
步骤2 | 35个循环 | 30秒 | 94℃ | |||
2μL | 10mM dNTPs* | 30秒 | 55℃ | |||
0.25ng | 每一引物 | 1分钟 | 68℃ | |||
1-5ng | 模板DNA | 步骤3 | 1个循环 | 10分钟 | 70℃ | |
1μL | Herculase DNA聚合酶* | 步骤4 | 1个循环 | 保持 | 4℃ | |
剩余物 | 蒸馏的去离子水(ddH2O) |
菌株(mtlE上游的区域大小,宿主) | 1%甘露糖醇 | 1%甘油 | 1%葡萄糖醇 | 1%甘露糖醇甘油 | 1%甘露糖醇葡萄糖醇 | 1%甘油 |
pDOW1365-1(299bpMB101)pDOW1365-1-2(299bpMB101)pDOW1369(126bpMB101)pDOW1377(147bpMB101) | ++-+ | --ndnd | -ndnd | ++-nd | ++ndnd | +nd-nd |
pDOW1378(77bp MB101)pDOW1370-7(203bp Pf0-1)pDOW1361对照(没有Cop基因) | --- | ndnd- | ndnd- | ndnd- | ndnd- | ndnd- |
hGH累积(mg/mL) | ||||
I24可溶 | I24不可溶 | I48可溶 | I48不可溶 | |
在24hr EFT诱导的hGH | 0.05 | 0.51 | 0.03 | 0.95 |
在24hr EFT*诱导的hGH,GrpE DnaKJ | 0.24 | 0.02 | 0.29 | 0.04 |
在24hr EFT诱导的hGH,在8hr EFT诱导的GrpEDnaKJ | 0.17 | 0 | 0.21 | 0 |
在24hrEFT诱导的GrpE,DnaKJ | 0 | 0 | 0 | 0 |
Claims (24)
Applications Claiming Priority (3)
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US68776305P | 2005-06-06 | 2005-06-06 | |
US60/687,763 | 2005-06-06 | ||
PCT/US2006/021958 WO2006133210A2 (en) | 2005-06-06 | 2006-06-06 | Mannitol induced promoter systems in bacterial host cells |
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CN101193906A true CN101193906A (zh) | 2008-06-04 |
CN101193906B CN101193906B (zh) | 2012-09-05 |
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US (2) | US7476532B2 (zh) |
EP (1) | EP1888763B1 (zh) |
JP (1) | JP5176114B2 (zh) |
KR (1) | KR101304921B1 (zh) |
CN (1) | CN101193906B (zh) |
AU (1) | AU2006255060B2 (zh) |
BR (1) | BRPI0611112A2 (zh) |
CA (1) | CA2610405C (zh) |
MX (1) | MX2007015355A (zh) |
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WO (1) | WO2006133210A2 (zh) |
Cited By (9)
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CN102471774A (zh) * | 2009-08-10 | 2012-05-23 | 龙沙股份公司 | 包含甘露糖启动子的载体和甘露糖启动子 |
CN103882047A (zh) * | 2014-03-18 | 2014-06-25 | 太原锦波生物医药科技有限公司 | 重组人酸性成纤维细胞生长因子蛋白的制备方法 |
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WO2016106504A1 (zh) * | 2014-12-29 | 2016-07-07 | 财团法人农业科技研究院 | 表达元件、表达盒、及含其的载体 |
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EP2722390B1 (en) | 2010-07-29 | 2017-05-10 | Lonza AG | Regulation of inducible promoters |
US20150353940A1 (en) | 2013-08-05 | 2015-12-10 | Absci, Llc | Vectors for use in an inducible coexpression system |
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US10508270B2 (en) | 2012-08-05 | 2019-12-17 | Absci Llc | Coordinated coexpression of thrombin |
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-
2006
- 2006-06-06 AU AU2006255060A patent/AU2006255060B2/en active Active
- 2006-06-06 CA CA2610405A patent/CA2610405C/en active Active
- 2006-06-06 JP JP2008515835A patent/JP5176114B2/ja active Active
- 2006-06-06 PL PL06772320T patent/PL1888763T3/pl unknown
- 2006-06-06 EP EP06772320.5A patent/EP1888763B1/en active Active
- 2006-06-06 KR KR1020087000198A patent/KR101304921B1/ko not_active IP Right Cessation
- 2006-06-06 BR BRPI0611112-2A patent/BRPI0611112A2/pt not_active IP Right Cessation
- 2006-06-06 MX MX2007015355A patent/MX2007015355A/es unknown
- 2006-06-06 CN CN2006800201108A patent/CN101193906B/zh active Active
- 2006-06-06 WO PCT/US2006/021958 patent/WO2006133210A2/en active Application Filing
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EP1888763A4 (en) | 2009-05-13 |
AU2006255060A1 (en) | 2006-12-14 |
PL1888763T3 (pl) | 2015-12-31 |
WO2006133210A2 (en) | 2006-12-14 |
WO2006133210A3 (en) | 2007-05-24 |
JP2008545436A (ja) | 2008-12-18 |
CA2610405C (en) | 2015-01-27 |
US20090162899A1 (en) | 2009-06-25 |
CN101193906B (zh) | 2012-09-05 |
MX2007015355A (es) | 2008-02-19 |
EP1888763B1 (en) | 2015-08-12 |
CA2610405A1 (en) | 2006-12-14 |
KR20080018937A (ko) | 2008-02-28 |
EP1888763A2 (en) | 2008-02-20 |
JP5176114B2 (ja) | 2013-04-03 |
AU2006255060B2 (en) | 2012-08-30 |
KR101304921B1 (ko) | 2013-09-13 |
BRPI0611112A2 (pt) | 2010-08-10 |
US8017355B2 (en) | 2011-09-13 |
US7476532B2 (en) | 2009-01-13 |
US20060292671A1 (en) | 2006-12-28 |
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