CN101147461A - Selenium enriched sprout vegetable cultivating method - Google Patents

Selenium enriched sprout vegetable cultivating method Download PDF

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CN101147461A
CN101147461A CNA2007101501214A CN200710150121A CN101147461A CN 101147461 A CN101147461 A CN 101147461A CN A2007101501214 A CNA2007101501214 A CN A2007101501214A CN 200710150121 A CN200710150121 A CN 200710150121A CN 101147461 A CN101147461 A CN 101147461A
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bud
selenium
seedling
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刘丽丽
窦羿
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Tianjin Normal University
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Tianjin Normal University
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Abstract

The present invention relates to a cultivation method of selenium-enriched sprout seedling vegetable. Said cultivation method includes the following steps: selecting seed for cultivating sprout seedling vegetable, soaking seed, sprouting, applying fertilizer, illumination management, water content management and picking. In particular, said invention utilizes the above-mentioned method to cultivate green radish sprout seedling regetable containing high selenium content.

Description

A kind of cultivating method of selenium enriched sprout vegetable
Technical field
The invention belongs to the vegetable growing technical field, relate to cultivating method nuisanceless, the noresidue vegetables, the cultivating method of a kind of selenium enriched sprout vegetable of saying so more specifically.
Background technology
Along with the raising of people's living standard, favored by the civic to kind and the quality of vegetables and the requirement vegetables more and more higher, particularly nuisanceless, noresidue that keep healthy.Bud seeding kind is a kind of novel vegetables, and it is to utilize plant seed or other nutrition organs to develop into children tender bud, seedling or stem etc. as edible a kind of vegetables special vegetable under adapt circumstance.Bud seeding kind just comes into vogue in the early 1990s at home, it contains the not available nutrition of many conventional vegetables, particularly it can be converted into the material that human bodies such as amino acid, mineral matter, vitamin easily absorb to the vegetable protein that many human bodies are difficult to absorb after cultivation, even most bud seeding kind also has the effect of Dietotherapy double-purpose, and the suitableeest the elderly is as health food.
Selenium is the necessary trace element of human body, and selenium enters behind the human body and forms the selenium enzyme with combined with protein, and it has destroys and remove interior free yl, delays senility, and combine formation metallic selenium albumen composition with in-vivo metal mercury, lead, cadmium etc. and detoxifies.Selenium can be removed a large amount of poisonous substances and the refuse that body metabolism produces, destruction is at the cholesterol of arterial blood tube wall deposition, prevent arterial sclerosis, hypertension, coronary heart disease and other cardiovascular and cerebrovascular disease, the medical personnel is adopting selenium to prevent and treat hepatitis B, mend the selenium treatment to the patient, can in the relatively short time, improve its poor appetite greatly, obviously weak, symptoms such as face is gloomy, every index of patient's hepatitis B is also reduced to normally very soon.Because the main biologically active of selenium and human health and animals and plants metabolism growth are in close relations, the medical value in selenium ore deposit has been subjected to generally paying attention to.Normal person's minimum daily requirements every day is 40 μ g, but the distribution of selenium is very unequal, and most of soil lacks selenium, causes agricultural product to lack selenium, adds in China's dietary structure, and the ratio that animal food, marine product account for is less, is easy to cause that human body lacks selenium; And then causing diseases of cardiovascular and cerebrovascular systems, cancer patient increases.In order to mend selenium to human body, people design a lot of ways, and have developed various selenium-rich nutritive health products.But because of price is too high, the very difficult expense of bearing great number for a long time of general family, can not be regular take causes most of people to rest in the troop that lacks selenium.
Selenium enriched sprout vegetable is exactly by bioconversion method, in the self-sow process of plant, organically the selenium element is imported in the plant corpus, thereby produces the higher bud seeding kind of organic selenium content.Therefore " rich selenium bud dish " not only provides the nutriment of needed by human body with the form of vegetables, and can not cause that as a kind of good selenium supplementary source (the highest safe intake of selenium is 400ug/d to selenosis, and the boundary dosis toxica of selenium is 800ug/d.)。The data show that world FAO, WHO, three international organizations of IAEA announce: the absorption 50～250ug of normal person's selenium every day, both can satisfy the normal psychological need of human body.
Summary of the invention
The objective of the invention is to, overcome above-mentioned shortcoming, provide a kind of cheap, the simple selenium enriched sprout vegetable cultivating method of method with not enough.The cultivation method of the rich selenium of green turnip seedling dish particularly.
The inventor is surprised to find in test: become fertile tender growth of seedlings in the phase at seed germination, spray the certain density sodium selenite or the sodium selenate aqueous solution, by specific cultivation the bud seeding kind Se content is increased, thereby reach the purpose that improves the rich selenium of bud seeding kind.The inventor further discovers: what big difference is the concentration that bud seeding kind applies the sodium selenite or the sodium selenate aqueous solution have because of the kind difference of bud seedling to the sensitivity of selenium.In order to determine the sodium selenite or the sodium selenate aqueous solution the concentration that applies accurately to different bud seeding kind, the inventor has carried out a large amount of trial tests, under the quality prerequisite that guarantees plant, further pursue the Se content in the plant, finished experiment work of the present invention finally.
Technical scheme of the present invention is as follows:
A kind of cultivating method of selenium enriched sprout vegetable, this method is undertaken by the seed selection of bud seedling, seed soaking, vernalization, fertilising, illumination management, water management, each step of gathering, and it is characterized in that:
(1) vernalization: get and respectively coil seed and eluriate 2-3 time, evenly sow in the seedling-cultivating tray, the seedling dish is stacked together, highly be no more than 6 dishes, go up one most and coil and add a cover cardboard and carry out shading with clear water;
(2) fertilising: behind vernalization 48h, spray 1 time every 4h, the sodium selenite or the sodium selenate aqueous solution of 0.02～40mg.L-1 concentration of each 10-20mL spray 3-5d;
(3) gather: as bud seedling cultivation 6-7d, whole strain harvesting when growing to 6-10cm is measured selenium constituent content in the bud seeding kind.
Cultivating method of the present invention, wherein preferred fertilising is: behind the bud seedling sowing 2d, spray 5～30mg.L -1The sodium selenite or the sodium selenate aqueous solution.Be more preferably 10-20mg.L -1, be preferably the sodium selenite or the sodium selenate aqueous solution that spray 20mg.L-1 especially.
Bud seeding kind of the present invention comprises: radish, pumpkin, balsam pear, peanut, red bean, black soya bean, rde bean, pea, soya bean, mung bean, wheat, barley, water spinach, broad bean, Chinese toon, pea, yellow mustard, buckwheat or clover bud seedling, preferred bud seeding kind comprises radish, peanut, black soya bean, rde bean, pea, soya bean, wheat or the like.Cultivating method of the present invention, according to the specificity of bud seeding kind itself, the concentration that sprays the sodium selenite or the sodium selenate aqueous solution has very big otherness.Generally can consider: 0mg.L -1, 40mg.L -1, 80mg.L -1, 160mg.L -1, 320mg.L -1, 700mg.L -1, but test finds to work as the concentration of selenium greater than 40mg.L -1The time, the moyashi growth is subjected to severe inhibition.Therefore, to design the level of selenium solution be 0mg.L in the present invention -1, 5mg.L -1, 10mg.L -1, 20mg.L -1, 30mg.L -1, 40mg.L -1
The typical green turnip bud of the present invention seedling cultivating method: the concentration of preferably applying fertilizer is: behind the green turnip bud seedling sowing 2d, spray sodium selenite or sodium selenate concentration of aqueous solution at 2～20mg.L -1In green turnip bud seedling process of growth, what be more preferably applies external source selenium concentration of element and can choose 20mg.L -1, this moment is to the accumulation maximum of selenium.
Another typical moyashi seedling cultivating method of the present invention: the concentration of preferably applying fertilizer is: behind the moyashi seedling sowing 2d, spray sodium selenite or sodium selenate concentration of aqueous solution at 8～30mg.L -1Effect is better.Be more preferably the external source selenium concentration of element that applies in the moyashi seedling process of growth and can choose 30mg.L -1, this moment is to the accumulation maximum of selenium.
For the actual effect of the selenium enriched sprout vegetable that adopts cultivating method cultivation of the present invention can more clearly be described, existing with rich selenium green turnip bud seedling cultivation and assay method as the in addition detailed description of exemplary embodiments.
1 test material:
723-PC type spectrophotometer, acidometer, analytical balance, the portable photosynthetic analyzer of LI-6400, green turnip (Raphanus satirus L) seed (purity 90%, cleanliness 97%, germination rate 96% is purchased in Tianjin Shennong Seed Co., Ltd.), seedling-cultivating tray (long 62cm * wide 23.6cm * high 5cm), culture dish, high tube beaker, the concentrated sulfuric acid, red fuming nitric acid (RFNA), buffer solution (borax soln of pH 9.18), HCl, HClO 4, pure selenium powder (99.99%, analyze pure), Na 2SeO 3, KI solution (analyzing pure), NaAc solution (analyzing pure), malachite green solution (analyzing pure).
2 test methods
(1) cultivation of green turnip bud seeding kind
Test is carried out under 27 ℃ of left and right sides conditions of indoor temperature all the time, selects good green turnip seed for use, and 6 parts of every part of 1g of weighing eluriate 3 times with clear water, are placed on respectively in sterilized 6 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 12h.Get and respectively coil seed and eluriate 3 times with clear water, evenly sow in the seedling-cultivating tray, 6 dishes stack together, go up a dish most and add a cover cardboard and carry out shading, placing behind the vernalization 48h.The bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination and cultivates behind the 24h bud seedling and place indoor intensity of illumination about 3000Lx and the intensity of illumination homogeneous area behind the placing.Spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.Whole strain harvesting when the bud seedling is cultivated 6-7d.
(2) preparation of selenium solution and applying method
The compound method of selenium solution: selenium is with Na 2SeO 3Form applies, and calculated mass concentration is with Na 2SeO 3The quality meter.Preliminary experiment once designed 5 level: 0mg.L -1, 40mg.L -1, 80mg.L -1, 160mg.L -1, 320mg.L -1, 700mg.L -1, find to work as the concentration of selenium greater than 40mg.L -1The time blastogenesis length be subjected to severe inhibition.So the level of this experimental scheme selenium solution is 0mg.L -1, 5mg.L -1, 10mg.L -1, 20mg.L -1, 30mg.L -1, 40mg.L -1
The applying method of selenium solution: behind the sowing 2d, spray 1-2 time every 4h, each 10-20mL sprays 3-5d.
(3) the green turnip bud seeding kind whole strain harvesting when growing to the 10cm left and right sides of gathering.
(4) assay method of the apparent index of bud seeding kind adopts observational technique directly perceived.
(5) mensuration of selenium constituent content in the radish bud seeding kind
Round the strain bud seeding kind and embathe 2h, use deionized water rinsing 3 times, put into freeze-day with constant temperature 10h in 80 ℃ of physics and chemistry drying boxes immediately, be ground into dry powder then, this is a testing sample.Testing sample is digested.Use HCI-HNO 3-HCIO 4Digestion method.HCl: HNO wherein 3: HClO 4Than being 1: 3: 6 (each reagent is and analyzes pure medicine).
(6) ultraviolet spectrophotometry is surveyed the content of selenium element in the green turnip bud seeding kind
Take by weighing selenium standard liquid (5ug.mL -1) 0.00,0.25,0.50,1.00,1.50,2.00mL, place the 25mL colorimetric cylinder respectively, add 4mol/L HCl solution 2mL, 1mol/L KI solution 2.5mL, 2mol/L NaAc solution 3mL, 1% poly-vinyl alcohol solution 2mL and 0.04% malachite green solution 2mL successively.Adding distil water is diluted to scale, shakes up, and places 20min.With the reagent blank is reference, with 731nm is to detect wavelength, measures absorbance.With selenium concentration in the standard selenium solution trap is returned.
Accurately take by weighing sample 0.2g and digest in conical flask, be placed in the 10mL measuring bottle, adding distil water is to scale.(selenium concentration is 5ug.mL accurately to pipette above-mentioned digestive juice and selenium standard liquid respectively -1) 1.0ml in the 25mL colorimetric cylinder, add 4mol.L successively -1HCl solution 2.0mL, 1mol.L -1KI solution 2.5mL, 2mol.L -1NaAc solution 3.0mL, 2.0mL 1% poly-vinyl alcohol solution, 0.04% malachite green solution 2.0ml uses the distilled water constant volume, shakes up.Use the 1cm cuvette,, do reference with reagent blank and measure absorbance, calculate the content of selenium in the sample according to the selenium calibration curve at wavelength 731nm place.
(7) mensuration of chlorophyll content
Remove the dust of green turnip bud seeding kind blade surface with writing brush or hairbrush, get the greenery sample, the 0.1g that weighs immediately puts into digestion agent (10mL acetone+10mL 95% ethanol) and leaves standstill lixiviate 12h after shredding, treat that residue all bleaches after.Getting thickness is the clean cuvette of 1cm, cleans 2～3 times with a small amount of extract earlier, then pigment extract is poured in the cuvette, and liquid level is about 4/5 of cuvette height.With the digestion agent is blank, measures the absorbance of solution at 663nm, 645nm respectively.Each sample replication 3 times.
(8) fixed light source photometric curve
Sowing 2d, every 48h surveys the fixed light source light curve, surveys altogether 3 times.Use LI-6400 photosynthesis measurement instrument to measure, do not carry out CO 2Concentration and temperature controlling are by the setting of apparatus measures program: set intensity gradient from high to low; At 0～2000umol.m -2.s -1In the scope, with photosynthetic active radiation (led light source) setting 2000,1700,1400,1200,1000,800,600,400,200,150,100,50,20,0umol.m -2.s -1, the measurement stand-by period is made as 180S.Automatically process of measurement generates after matched curve obtains relevant parameter.
3 results
(1) selenium calibration curve
Draw out selenium element calibration curve by the 2.5.2.1 method and see Fig. 1.With selenium concentration in the standard selenium solution trap is returned, get linearity of regression equation Y=0.8173x+0.0025 (ug.mL -1), coefficient R 2=0.9982.Abscissa shows the selenium constituent content among the figure, the ug.mL-1 of unit, and ordinate shows light absorption value.Se content is at 0～0.40ug.mL -1Obey Beer law.
(2) different disposal mass concentration selenium and selenium accumulation relation
Handling the green turnip bud seeding kind that obtains according to the preparation of 2.2 selenium solutions and applying method records the selenium accumulation via the 2.5.2 method and sees Fig. 2.Abscissa is represented Na among the figure 2SeO 3The concentration that applies, the mg.L of unit -1, ordinate is represented selenium element accumulation amount in the green turnip bud seedling, and selenium concentration is at 0～40mg.L -1In the scope, also increase with the accumulation of the selenium concentration increase radish bud dish that applies to selenium, both present good positive correlation.
(3) different disposal mass concentration selenium is to the influence of green turnip bud seedling output etc.
Mensuration sprays varying level concentration Na 2SeO 3Apparent indexs such as green turnip bud seedling sample bud is long, the bright quality of individual plant, individual plant dry mass, root length, single bright quality see Table 1.
Table 1: spray of the influence of different quality concentration sodium selenite to green turnip bud seeding kind plant strain growth
Na 2SeO 3Concentration mg.L -1 Bud length/cm Individual plant (bright quality)/g Individual plant (dry mass)/g Root length/(cm) Single bright quality/(g)
0 5 10 20 30 40 9.22 8.78 8.48 8.35 * 8.05 * 7.56 ** 0.156 0.148 0.141 0.133 0.112 * 0.096 ** 0.013 0.014 0.013 0.012 0.0098 * 0.0099 * 5.22 5.18 5.09 5.02 4.83 * 4.67 * 0.088 0.083 0.082 0.079 0.068 * 0.067 *
Annotate: data are 30 strain mean values in the table, *, * * represent with the SPSS software analysis have significantly, utmost point significance level.
Table 1 shows each level concentration Na 2SeO 3Green turnip bud seedling sample 30 strain buds are long, the bright quality of individual plant, individual plant dry mass, root long, single bright mass average value, by SPSS software each group data analyzed 20mg.L -1Na 2SeO 3Green turnip bud seedling bud length is had obvious inhibitory action, and to the bright quality of individual plant, individual plant dry mass, root is long, single bright quality influence is less; Spray Na 2SeO 3Concentration is higher than 20mg.L -1, the bright quality of individual plant, individual plant dry mass, root length, single bright quality all have decline significantly.Test chooses 5,10,20mg.L -1Na 2SeO 3Spray in green turnip bud seedling process of growth, output difference compared with the control is not remarkable; Increase Na 2SeO 3Concentration, output reduces.Explanation is under this experiment condition, and green turnip bud seedling can be stood 20mg.L -1Na 2SeO 3
(4) selenium is to the influence of green turnip bud seedling physio-biochemical characteristics
1. selenium is to the influence of green turnip bud seedling chlorophyll content
Tentatively think 20mg.L by the mensuration of selenium accumulation -1Be to obtain the comparatively ideal level concentration of rich selenium green turnip bud seedling, measure Spraying Se concentration 0,20mg.L according to method in 2.6 -1Total chlorophyll, chlorophyll a, chlorophyll b content in the green turnip bud seedling leaf, and with SPSS software each is organized data and analyze, the results are shown in Figure 2.
Spray different quality concentration Na 2SeO 3Influence to radish bud seeding kind chlorophyll content.Abscissa represents to apply the concentration of selenium among the (see figure 3) figure, the mg.L of unit -1, ordinate is represented chlorophyll a in the sample, chlorophyll b and total chlorophyll content, and the ug.g-1 of unit sprays 20mg.L-1Na2SeO3 green turnip bud seedling compared with the control, and chlorophyll a, chlorophyll b and total chlorophyll content all do not have significant difference.
2. selenium is to the influence of green turnip bud seedling photosynthetic rate etc.
By the mensuration of selenium accumulation and the mensuration of sample chlorophyll content, think 20mg.L -1Be to obtain the comparatively ideal level concentration of rich selenium green turnip bud seedling,, measure and apply 20mg.L according to 2.7 methods -1Green turnip bud seedling and control sample fixed light source light curve are set intensity gradient from high to low; At 0～2000umol.m -2.s -1In the scope, with photosynthetic active radiation (led light source) setting 2000,1700,1400,1200,1000,800,600,400,200,150,100,50,20,0umol.m -2.s -1, to measure the stand-by period to be made as 180S, every group of parameter record data of averaging the results are shown in Table 2.
Table 2: spray different quality concentration Na2SeO3 to the photosynthetic influence of radish bud seeding kind
1.CK 1.20mg.L -Selenium is handled 2.CK 2.20mg.L -Selenium is handled 3.CK 3.20mg.L -Selenium is handled
Photo (photosynthesis rate/μ mol CO 2m -2s -1) Cond (stomatal conductance/mol H 2O m -2s -1) Ci (intercellular CO 2Concentration/μ mol CO 2mol -1) Trmmol (transpiration rate/mmol H 2O m -2s -1) 0.98 0.67 461 2.82 1.07 0.78 467 2.88 1.13 0.723 467 2.67 1.18 0.762 468 2.59 1.32 0.891 478 3.26 1.49 0.857 486 3.42
Every 48h records parameters such as photosynthesis rate, stomatal conductance, intercellular CO2 concentration, transpiration rate after having listed sowing 2d in the table 2, surveys 3d altogether, by sequence number 1,2,3 expressions, sprays 20mg.L -1Na 2SeO 3Green turnip bud seedling leaf photosynthesis rate, stomatal conductance, intercellular CO 2Concentration, transpiration rate there is no significant difference compared with the control.
4. analyze and discuss
Selenium concentration is at 0～40mg.L -1In the scope, also increase with the accumulation of selenium concentration increase green turnip bud dish to selenium.Green turnip bud length is subjected to the inhibitory action sensitivity of external source selenium, 20mg.L -1Sodium selenite spray processing and can cause the long remarkable reduction of bud, 40mg.L -1Processing reaches utmost point significance level; And the bright quality of bud dry mass, bud, root are long, single bright quality is at 30mg.L -1Handle under the mass concentration and significant difference just occurs.Handle mass concentration and be higher than 30mg.L -1, the bright quality of bud length, bud dry mass, bud, root are long, the bright quality of root all has significantly and descends.
Selenium processing mass concentration reaches certain level concentration may produce single salt murder by poisoning, the selenium that applies high concentration can influence the quality of bud seedling, and selenium is handled the total chlorophyll of mass concentration 20mg.L-1 green turnip bud Miao, chlorophyll a, chlorophyll b content and do not had significant difference compared with the control.Show that the selenium that applies under this level concentration can be to the synthetic considerable influence that produces of green turnip bud seedling chlorophyll.Handle mass concentration 20mg.L according to table 2 selenium -1Photosynthesis rate, stomatal conductance, intercellular CO2 concentration, transpiration rate that the bud seedling is measured for three times do not have compared with the control than big-difference.From single factorial analysis, each factor is all relevant with photosynthetic rate, though each factor is not unique factor of influence, can prove from the side, and the external source selenium that applies under an amount of this level concentration can not produce considerable influence to green turnip bud seedling photosynthesis.According to the above, the measurement result of chlorophyll content and fixed light source light curve further illustrates from the side, and the selenium level concentration that this experiment is chosen is reasonable, and method is feasible.According to above-mentioned analysis, the external source selenium concentration of element that applies in green turnip bud seeding kind process of growth can be chosen 20mg.L -1
The cultivating method technology of selenium enriched sprout vegetable of the present invention is simple, with low cost, workable, resulting bud seeding kind, cotyledon is fresh and tender, abundance is loose, not only kept tasty and refreshing, the pleasant aid digestion function of common green turnip, and it is because of having accumulated selenium-containing compound, the selenium amount of green turnip bud seeding kind is high, and every day, edible 70～340g can reach the effect of mending selenium.Not only satisfied the demand that broad masses of the people mend selenium, the while also provides economic rich vegetables for common people's dining table.
Description of drawings:
Fig. 1 is: the Se content calibration curve.
Fig. 2 is: Se element accumulation amount.
Fig. 3 is: spray different quality concentration Na 2SeO 3Influence to radish bud seeding kind chlorophyll content.
Embodiment:
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
The cultivating method of rich selenium green turnip bud seeding kind:
(1) seed selection: select purity, cleanliness good, germination rate height, the green turnip bud seed of full seed.
(2) seed soaking: in sowing seed is cleaned, reject damage by worms, broken residual, lopsided, rotten, flat, special granule and seeds germinated.Select good green turnip seed for use, 6 parts of weighings, every part of 1g eluriates 3 times with clear water, is placed on respectively in sterilized 6 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 12h, indoor temperature is controlled at about 27 ℃ all the time.
(3) vernalization: get the dish seed and eluriate 3 times with clear water, evenly sow in the seedling-cultivating tray, 6 dishes stack together, go up a dish most and add a cover cardboard and carry out shading, placing behind the vernalization 48h.
(4) applying method of selenium solution: selenium is with Na 2SeO 3Form applies, and calculated mass concentration is with Na 2SeO 3The quality meter.Behind the sowing 2d, spray 20mg.L -1, spraying once every 4h, each 10mL sprays 3d, under the quality prerequisite that guarantees plant, further pursues the Se content in the plant.
(5) illumination management: behind the placing, the bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination cultivate 24h after, the bud seedling places indoor intensity of illumination about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) whole strain harvesting when the bud seedling is cultivated 6-7d of gathering, fresh and tender, the abundant hypertrophy of cotyledon.Measure green turnip bud seeding kind Se content then, the results are shown in Figure 1-3.
Embodiment 2
The cultivating method of rich selenium green turnip bud seeding kind:
(1) seed selection: select purity, cleanliness good, germination rate height, the green turnip bud seed of full seed.
(2) seed soaking: select good green turnip seed for use, 6 parts of every part of 1g of weighing eluriate 2 times with clear water, are placed on respectively in sterilized 6 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 24h.
(3) vernalization: get the dish seed and eluriate 3 times with clear water, evenly sow in the seedling-cultivating tray, 6 dishes stack together, go up a dish most and add a cover cardboard and carry out shading, placing behind the vernalization 48h.
(4) applying method of selenium solution: selenium is with Na 2SeO 3Form applies, and calculated mass concentration is with Na 2SeO 3The quality meter.Behind the sowing 2d, spray 10mg.L -1, spraying once every 4h, each 10mL sprays 3d, under the quality prerequisite that guarantees plant, further pursues the Se content in the plant.
(5) illumination management: behind the placing, the bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination cultivate 24h after, the bud seedling places indoor intensity of illumination about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) whole strain harvesting when the bud seedling is cultivated 6-7d of gathering, fresh and tender, the abundant hypertrophy of cotyledon.Measure green turnip bud seeding kind Se content then, the results are shown in Figure 1-3.
Embodiment 3
The cultivating method of rich selenium soya bean bud seeding kind:
(1) seed selection: select purity, cleanliness good, germination rate height, the moyashi seed of full seed.
(2) seed soaking: select good moyashi seed for use, 10 parts of every part of 2g of weighing eluriate seed 2-3 time with 20-30 ℃ clean clear water earlier, are placed on respectively in sterilized 10 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 8h.Seed soaking is eluriated seed 2-3 time after finishing again, and drop removes excessive moisture.
(3) vernalization: under 25 ℃ of cultivating chambers, get the dish seed and eluriate 3 times with clear water, evenly sow in the seedling-cultivating tray, per 5 dishes stack together, go up a dish most and add a cover cardboard and carry out shading, placing behind the vernalization 24h.
(4) applying method of selenium solution: selenium is with Na 2SeO 3Form applies, and calculated mass concentration is with Na 2SeO 3The quality meter.Behind the sowing 2d, spray 30mg.L -1, spraying once every 4h, each 10mL sprays 3d, under the quality prerequisite that guarantees plant, further pursues the Se content in the plant.
(5) illumination management: behind the placing, the bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination and cultivates, and the bud seedling places the room light photograph behind the 24h, and intensity is about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) gather: when the bud seedling was cultivated 7d, 10 centimetres of heights of seedling, cotyledon launched, abundant hypertrophy, and neat undercut cuts when edible, measures soya bean bud seeding kind Se content.
Embodiment 4
(1) seed selection: select purity, cleanliness good, germination rate height, the green turnip bud seed of full seed.
(2) seed soaking: in sowing seed is cleaned, reject damage by worms, broken residual, lopsided, rotten, flat, special granule and seeds germinated.Select good green turnip seed for use, 10 parts of every part of 2g of weighing eluriate 3 times with clear water, are placed on respectively in sterilized 10 culture dishes, and the amount that adds water was not as the criterion there not to be seed, seed soaking 12h, and indoor temperature is controlled at about 27 ℃ all the time.
(3) vernalization: get and respectively coil seed and eluriate 3 times with clear water, evenly sow in the seedling-cultivating tray, per 5 dishes stack together, go up a dish most and add a cover cardboard and carry out shading, placing behind the vernalization 48h.
(4) applying method of selenium solution: selenium is with Na 2SeO 3Form applies, and calculated mass concentration is with Na 2SeO 3The quality meter.Behind the sowing 2d, spray 35mg.L -1, spraying once every 4h, each 10mL sprays 3d, under the quality prerequisite that guarantees plant, further pursues the Se content in the plant.
(5) illumination management: behind the placing, the bud seedling is placed on the more weak and intensity of illumination homogeneous area of indoor intensity of illumination cultivate 24h after, the bud seedling places indoor intensity of illumination about 3000Lx and the intensity of illumination homogeneous area.
(6) water management: spray 4 every day behind the placing, and matrix is moistening, seedling is tried to get to the heart of a matter little minim water degree of being to spray in the seedling dish of back.
(7) whole strain harvesting when the bud seedling is cultivated 6-7d of gathering, cotyledon is fresh and tender, abundance is loose.Measure the rich Se content of green turnip bud seeding kind then, the results are shown in Figure 1-3.
After the preferred embodiment that describes in detail, being familiar with this technology personage can be well understood to, can carry out various variations and modification not breaking away under above-mentioned claim and the spirit, all foundations technical spirit of the present invention all belongs to the scope of technical solution of the present invention to any simple modification, equivalent variations and modification that above embodiment did.And the embodiment that the present invention also is not subject in the specification to be given an actual example.

Claims (8)

1. the cultivating method of a selenium enriched sprout vegetable, this method is undertaken by the seed selection of bud seedling, seed soaking, vernalization, fertilising, illumination management, water management, each step of gathering, and it is characterized in that:
(1) vernalization: get and respectively coil seed and eluriate 2-3 time, evenly sow in the seedling-cultivating tray, the seedling dish is stacked together, highly be no more than 6 dishes, go up one most and coil and add a cover cardboard and carry out shading with clear water;
(2) fertilising: behind vernalization 48h, spray 1 time every 4h, the sodium selenite or the sodium selenate aqueous solution of 0.02～40mg.L-1 concentration of each 10-20mL spray 3-5d;
(3) gather: as bud seedling cultivation 6-7d, whole strain harvesting when growing to 6-10cm is measured selenium constituent content in the bud seeding kind.
2. cultivating method as claimed in claim 1, wherein said fertilising is: behind the bud seedling sowing 2d, spray 5～3 0mg.L -1The sodium selenite or the sodium selenate aqueous solution.
3. cultivating method as claimed in claim 1 behind the wherein said bud seedling sowing 2d, sprays 10-20mg.L -1The sodium selenite or the sodium selenate aqueous solution.
4. cultivating method as claimed in claim 1 behind the wherein said bud seedling sowing 2d, sprays 20mg.L -1The sodium selenite or the sodium selenate aqueous solution.
5. cultivating method as claimed in claim 1, wherein said bud seeding kind comprises: radish, pumpkin, balsam pear, peanut, red bean, black soya bean, rde bean, pea, soya bean, mung bean, wheat, barley, water spinach, broad bean, Chinese toon, pea, yellow mustard, buckwheat or clover bud seedling.
6. cultivating method as claimed in claim 5, wherein said bud seeding kind is: green turnip bud seeding kind or soya bean bud seeding kind.
7. cultivating method as claimed in claim 6, wherein said fertilising is: behind the green turnip bud seedling sowing 2d, spray sodium selenite or sodium selenate concentration of aqueous solution at 2～20mg.L -1
8. cultivating method as claimed in claim 6, wherein said fertilising is: behind the moyashi seedling sowing 2d, spray sodium selenite or sodium selenate concentration of aqueous solution at 8～30mg.L -1
CNA2007101501214A 2007-11-09 2007-11-09 Selenium enriched sprout vegetable cultivating method Pending CN101147461A (en)

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