CN101107365A - 从细胞材料分离核酸的简化方法 - Google Patents

从细胞材料分离核酸的简化方法 Download PDF

Info

Publication number
CN101107365A
CN101107365A CNA2005800366286A CN200580036628A CN101107365A CN 101107365 A CN101107365 A CN 101107365A CN A2005800366286 A CNA2005800366286 A CN A2005800366286A CN 200580036628 A CN200580036628 A CN 200580036628A CN 101107365 A CN101107365 A CN 101107365A
Authority
CN
China
Prior art keywords
nucleic acid
solid phase
described method
groups
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2005800366286A
Other languages
English (en)
Chinese (zh)
Inventor
H·阿哈万-塔夫第
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nexgen Diagnostics LLC
Original Assignee
Nexgen Diagnostics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexgen Diagnostics LLC filed Critical Nexgen Diagnostics LLC
Publication of CN101107365A publication Critical patent/CN101107365A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CNA2005800366286A 2004-09-16 2005-07-01 从细胞材料分离核酸的简化方法 Pending CN101107365A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/942,491 US20050106602A1 (en) 2003-11-17 2004-09-16 Simplified methods for isolating nucleic acids from cellular materials
US10/942,491 2004-09-16

Publications (1)

Publication Number Publication Date
CN101107365A true CN101107365A (zh) 2008-01-16

Family

ID=36119320

Family Applications (2)

Application Number Title Priority Date Filing Date
CNA2005800366286A Pending CN101107365A (zh) 2004-09-16 2005-07-01 从细胞材料分离核酸的简化方法
CNA2005800366290A Pending CN101076603A (zh) 2004-09-16 2005-07-05 从生物和细胞材料分离核酸的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CNA2005800366290A Pending CN101076603A (zh) 2004-09-16 2005-07-05 从生物和细胞材料分离核酸的方法

Country Status (9)

Country Link
US (1) US20050106602A1 (https=)
EP (1) EP1799847A4 (https=)
JP (1) JP2008513008A (https=)
KR (1) KR20070062557A (https=)
CN (2) CN101107365A (https=)
AU (1) AU2005290300A1 (https=)
CA (1) CA2580700A1 (https=)
IL (1) IL181974A0 (https=)
WO (1) WO2006036246A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103930546A (zh) * 2011-09-26 2014-07-16 凯杰有限公司 用于分离细胞外核酸的快速方法
CN107249752A (zh) * 2015-01-27 2017-10-13 赛库乐米克斯公司 用于磁性核酸提取的分层硅石薄片

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060234251A1 (en) 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
WO2009020609A2 (en) * 2007-08-06 2009-02-12 Nanogen, Inc. Isolation of nucleic acids molecules using modified solid supports
FR2933989B1 (fr) * 2008-07-16 2013-03-08 Commissariat Energie Atomique Procede de purification de microorganismes presents dans des echantillons liquides
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
US9695416B2 (en) 2012-07-18 2017-07-04 Siemens Healthcare Diagnostics Inc. Method of normalizing biological samples
WO2014043644A1 (en) 2012-09-17 2014-03-20 Alltech Associates, Inc. Functionalized particulate support material and methods of making and using the same
RU2015114330A (ru) 2012-09-17 2016-11-10 У.Р. Грейс Энд Ко.-Конн. Хроматографические среды и устройства
CA2920248C (en) 2013-08-05 2023-10-10 The Johns Hopkins University Fabrication of hierarchical silica nanomembranes and uses thereof for solid phase extraction of nucleic acids
US11229896B2 (en) 2014-01-16 2022-01-25 W.R. Grace & Co.—Conn. Affinity chromatography media and chromatography devices
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
FR3031063B1 (fr) * 2014-12-30 2017-02-10 Biomerieux Sa Complexe multicouches, procede de fabrication et utilisation du complexe
CN107921407B (zh) 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
JP6787916B2 (ja) 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
CN114787194A (zh) * 2019-09-18 2022-07-22 南科征途有限公司 使用核-壳-壳磁珠的设备系统和方法
WO2021231607A1 (en) * 2020-05-12 2021-11-18 Godx, Inc. Point of need diagnostic device and methods of use thereof
CN111804280A (zh) * 2020-06-11 2020-10-23 中国科学院合肥物质科学研究院 一种制备磁性纳米氨基复合物的方法、以及核酸提取方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4297337A (en) * 1979-04-13 1981-10-27 Corning Glass Works Solid-phase immunoassays using magnetic glass
US4233169A (en) * 1979-04-13 1980-11-11 Corning Glass Works Porous magnetic glass structure
US4395271A (en) * 1979-04-13 1983-07-26 Corning Glass Works Method for making porous magnetic glass and crystal-containing structures
DE3211309A1 (de) * 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
NO155316C (no) * 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
AU604684B2 (en) * 1986-03-20 1991-01-03 Gen-Probe Incorporated Method for releasing RNA and DNA from cells
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
US5091206A (en) * 1987-10-26 1992-02-25 Baxter Diagnostics Inc. Process for producing magnetically responsive polymer particles and application thereof
US5395688A (en) 1987-10-26 1995-03-07 Baxter Diagnostics Inc. Magnetically responsive fluorescent polymer particles
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
US5665582A (en) * 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5411730A (en) * 1993-07-20 1995-05-02 Research Corporation Technologies, Inc. Magnetic microparticles
US5986076A (en) * 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5942425A (en) 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
ATE347615T1 (de) * 1997-04-16 2006-12-15 Applera Corp Nukleinsäuresammlung
EP0920526B1 (en) * 1997-06-12 2004-11-24 Sloan-Kettering Institute For Cancer Research Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase
GB9717173D0 (en) * 1997-08-13 1997-10-22 Akzo Nobel Nv Solid phase supports
DE19736366A1 (de) * 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6214618B1 (en) * 1998-04-07 2001-04-10 Solohill Engineering, Inc. Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6780327B1 (en) * 1999-02-25 2004-08-24 Pall Corporation Positively charged membrane
US6514700B1 (en) * 1999-04-30 2003-02-04 Aclara Biosciences, Inc. Nucleic acid detection using degradation of a tagged sequence
DK1206571T3 (da) * 1999-05-04 2004-12-06 Ortho Clinical Diagnostics Inc Hurtig og effektiv indfangning af DNA fra pröve uden anvendelse af cellelyseringsreagens
GB2367818A (en) * 2000-10-11 2002-04-17 Kalibrant Ltd A linker and a method of chemical synthesis
US6803200B2 (en) * 2000-12-12 2004-10-12 Invitrogen Corporation Compositions and methods for the release of nucleic acid molecules from solid matrices
ES2322841T3 (es) 2001-02-16 2009-06-30 Promega Corporation Aislamiento y purificacion magnetica de acidos nucleicos.
EP1243649A1 (de) 2001-03-23 2002-09-25 GeneScan Europe AG Verfahren zur Isolierung von DNA
US6746608B2 (en) * 2001-06-12 2004-06-08 Prometic Biosciences, Inc. Use of adsorbent polymer particles in DNA separation
ES2320875T5 (es) * 2002-10-04 2012-10-30 Ge Healthcare Bio-Sciences Corp. Procedimientos y materiales para utilizar compuestos químicos como herramienta para el almacenamiento de ácidos nucleicos sobre medios de sistemas de purificación de ácidos nucleicos
US20040121336A1 (en) * 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
NZ545469A (en) * 2003-09-12 2009-11-27 Biocontrol Systems Inc Methods, compositions, and kits for the concentration and detection of microorganisms without a separate DNA extraction step
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103930546A (zh) * 2011-09-26 2014-07-16 凯杰有限公司 用于分离细胞外核酸的快速方法
CN113355321A (zh) * 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
CN113388605A (zh) * 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
CN107249752A (zh) * 2015-01-27 2017-10-13 赛库乐米克斯公司 用于磁性核酸提取的分层硅石薄片
CN107249752B (zh) * 2015-01-27 2019-12-13 赛库乐米克斯公司 用于磁性核酸提取的分层硅石薄片

Also Published As

Publication number Publication date
US20050106602A1 (en) 2005-05-19
WO2006036246A2 (en) 2006-04-06
CN101076603A (zh) 2007-11-21
JP2008513008A (ja) 2008-05-01
WO2006036246A3 (en) 2007-02-08
EP1799847A2 (en) 2007-06-27
EP1799847A4 (en) 2007-12-12
CA2580700A1 (en) 2006-04-06
KR20070062557A (ko) 2007-06-15
AU2005290300A1 (en) 2006-04-06
IL181974A0 (en) 2007-07-04

Similar Documents

Publication Publication Date Title
CN101107365A (zh) 从细胞材料分离核酸的简化方法
US20050136477A1 (en) Methods for isolating nucleic acids from biological and cellular materials
CN101203758A (zh) 增强生物样品中rna分离的方法
US20050106589A1 (en) Compositions and methods for releasing nucleic acids from solid phase binding materials
CN101535501A (zh) 提取核酸的方法
US20050106576A1 (en) Methods of using cleavable solid phases for isolating nucleic acids
US20050106577A1 (en) Cleavable solid phases for isolating nucleic acids
CN101400689A (zh) 提取rna的方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20080116