CN101040049B - 胁迫抗性植物 - Google Patents
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Abstract
通过使用编码参与NAD补救合成途径和/或NAD从头合成途径的酶的核苷酸序列(如在植物中过表达)使植物和植物细胞获得胁迫耐受性。
Description
技术领域
(本发明)通过在植物中表达参与NAD补救合成途径或NAD从头合成途径中的酶，提供了增加植物或植物细胞胁迫抗性的方法。
技术背景
考虑到对最终增加作物植物的实际产率的永无止境的需求，对不利生长条件的耐受性是这些作物非常理想的特性，所述不利生长条件包括干旱、高光照强度、高温、营养限制、盐生长条件等等。
达到提高通常称为植物胁迫抗性或胁迫耐受性目的的多种方法已有描述。由于不同的非生物胁迫条件经常导致产生有害的活性氧(ROS))，如超氧化物或过氧化氢，因此，提高植物耐受性的初期尝试集中在阻止产生ROS或将其除去。这些方法的实施例是过表达ROS清除酶，如过氧化氢酶、过氧化物酶、超氧化物歧化酶等，或者甚至增加ROS清除分子，如抗坏血酸、谷胱甘肽等的量。产生胁迫耐受性植物的这些方法和其它尝试综述于，如wang等人，2003，Planta 218：1-14。
如WO00/04173和PCT/EP2004/003995中分别描述的，植物细胞和植物的胁迫耐受性也可以通过减少内源聚ADP核糖聚合酶(ParP)或聚(ADP-核糖)糖苷水解酶(ParG)的活性或水平而实现。一般认为，在这种方法中，遭受胁迫条件的植物细胞中，导致创伤性细胞死亡的致死的NAD和ATP衰竭可以被避免或充分地推迟，以使受胁迫的细胞存活并适应胁迫条件。
Uchimiya等人(2002)描述了命名为YK1的稻基因的分离，以及嵌合YK1基因增加含有这种基因的转基因稻类植物对稻瘟病和几种非生物胁迫(如NaCl、UV-C、浸没和过氧化氢)耐受性的用途(Uchimiya等人.，2002，molecular breeding 9：25-31)。
Uchimiya等人还发表一篇会议摘要，描述的是在稻细胞中过表达NAD依赖性还原酶基因(YK1)还通过上调NAD合成酶的活性提高NAD(P)(H)的水平，并且推断这种改变能依次产生ROS胁迫抗性所需要的氧化还原物质库(Uchimiya等人.2003Keystone symposium on Plantbiology：Functions and control of cell death，Snowbird Utah April 10-15，2003).
酵母中的NAD合成酶已经得到很好的表征，而且它是NAD从头合成途径以及NAD补救途径中最后的一个酶(见图1)。在从头合成途径中，喹啉(quinolate)是合成NAD的前体，并且是作为色氨酸降解产物产生。在补救途径中，烟酰胺是前体分子，其为通过多种酶(如PARP、NAD依赖的脱乙酰酶或其它NAD糖苷水解酶)作用产生的NAD的降解产物。在第一步中，烟酰胺由烟酰胺酶脱去酰胺基变成烟酸。烟酸在烟酸酯磷酸核糖基转移酶的作用下转移到5-磷酸核糖基-1-焦磷酸上产生烟酸单核苷酸。这一化合物是NAD从头合成途径和补救合成途径共同拥有的。因此，在从头合成途径中通过NAD+焦磷酸化酶和NAD合成酶完成这一化合物的进一步转化。
在酵母中，已经将过表达PNC1(编码烟酰胺酶)通过卡路里限制和低强度胁迫与生命期延长相关联(Anderson等人，2003 Nature 423：p181-185；Gallo等人，2004，Molecular and Cellular Biology 24：1301-1312).
对植物中NAD生物合成途径中的各个酶所知甚少。Hunt等人2004年描述了使用可获得的拟南芥基因组信息鉴定这些酶的植物同源物(Hunt等人，2004，New Phytologist 163(1)：31-44)。已经识别的DNA序列有以下的登录号：烟酰胺酶：At5g23220、At5g23230和At3g16190；烟酸酯磷酸核糖基转移酶：At4g36940、At2g23420；烟酸单核苷酸腺苷转移酶：At5g55810以及NAD合成酶：At1g55090(所有的核苷酸序列在此引用作为参考)。
依然需要能增加植物胁迫抗性的备选方法，下面描述的实施方案，包括权利要求，提供了这样的方法和手段。
发明概述
在本发明的一个实施方案中，提供了获得具有增加的胁迫抗性植物的方法，所述方法包括将嵌合基因引入植物细胞中获得转基因细胞，所述嵌合基因包括以下有效连接的DNA片段：
i.可在植物中表达的启动子；
ii.编码烟酰胺腺嘌呤二核苷酸补救合成途径中植物功能酶的DNA区，所述酶选自烟酰胺酶、烟酸酯磷酸核糖基转移酶，烟酸单核苷酸腺苷转移酶或烟酰胺腺嘌呤二核苷酸合成酶；
iii.参与转录终止和多聚腺苷化作用的3’末端区。
随后，由转基因植物细胞再生获得转基因植物群；并从转基因植物群选择比类似的非转基因植物表现出增加的胁迫抗性的植物，或在胁迫条件下表现出降低水平活性氧的植物或维持高水平NADH的植物。DNA区可以编码含有选自SEQ ID No.：2、SEQ ID No.：4、SEQ ID No.：6、SEQ IDNo.：8、SEQ ID No.：10、SEQ ID No.：12、SEQ ID No.：14、SEQ ID No.：16、SEQ ID No.：18、SEQ ID No.：20、SEQ ID No.：22、SEQ ID No.：24氨基酸序列的蛋白质；或编码具有约60％序列同一性并且具有烟酰胺腺嘌呤二核苷酸补救合成途径中的酶活性的蛋白质，如核苷酸序列SEQ ID No.：1、SEQ ID No.：3、SEQ ID No.：5、SEQ ID No.：7、SEQ ID No.：9、SEQ IDNo.：11、SEQ ID No.：13、SEQ ID No.：15、SEQ ID No.：17、SEQ ID No.：19、SEQ ID No.：21、或SEQ ID No.：23。
在另一实施方案中，本发明涉及这里所述的嵌合基因、包含这些嵌合基因的植物细胞、基本上由含有这些嵌合基因的植物细胞组成的植物以及这样的植物的种子。这些植物和植物细胞的特征在于其在胁迫条件下比类似的不包含这样的嵌合基因的植物拥有更低水平的活性氧。
而在另一个实施方案中，本发明涉及使用所述的嵌合基因以增加植物的胁迫抗性，或减少在胁迫条件下植物或植物细胞中活性氧的水平。
本发明还提供编码烟酰胺腺嘌呤二核苷酸补救合成途径中的植物功能酶的DNA序列的用途(所述酶选自烟酰胺酶、烟酸酯磷酸核糖基转移酶、烟酸单核苷酸腺苷转移酶或烟酰胺腺嘌呤二核苷酸合成酶)，例如编码含有选自SEQ ID No.：2、SEQ ID No.：4、SEQ ID No.：6、SEQ ID No.：8、SEQID No.：10、SEQ ID No.：12、SEQ ID No.：14、SEQ ID No.：16、SEQ IDNo.：18、SEQ ID No.：20、SEQ ID No.：22、SEQ ID No.：24氨基酸序列的蛋白质的DNA序列，或编码具有约60％序列同一性并且具有烟酰胺腺嘌呤二核苷酸补救合成途径中酶活性的蛋白质的DNA序列，包括含有选自SEQ ID No.：1、SEQ ID No.：3、SEQ ID No.：5、SEQ ID No.：7、SEQ ID No.：9、SEQ ID No.：11、SEQ ID No.：13、SEQ ID No.：15、SEQ ID No.：17、SEQ IDNo.：19、SEQ ID No.：21、或SEQ ID No.：23核苷酸序列的DNA序列，所述用途为在胁迫条件下增加植物胁迫抗性，或减少活性氧水平，或维持植物或植物细胞中的NADH水平。
附图简述
图1是面包酵母(酿酒酵母(Saccaromyces cerevisiae))中公知的NAD补救合成途径和NAD从头合成途径的图示。
图2-11是多种T-DNA载体的图示，这些T-DNA载体包含可在植物中表达的控制元件的控制下编码NAD补救合成途径或NAD从头合成途径中的酶的DNA区。使用的缩写为：RB：T-DNA右边界；3’35S：来自花椰菜花叶病毒35S转录物的转录终止和多聚腺苷酸化作用信号；Cab22L：Cab22L转录物的非翻译前导序列；P35S2：花椰菜花叶病毒35S启动子；3’g7：来自根瘤农杆菌(Agrobacterium tumefaciens)T-DNA基因7的转录终止和聚腺甘酸信号；bar：膦丝菌素乙酰转移酶编码区；pSSUAra：来自拟南芥的核酮糖二磷酸羧化酶加氧酶小亚基转录物的启动子；LB：T-DNA左边界；Sm/Sp：壮观霉素和链霉素抗性基因；pVS1ori：适合在农杆菌中复制的VS1起始点；ColE1：复制起点；NLS：核定位信号；PNC1：编码酿酒酵母烟酰胺酶的DNA区；npt1：酿酒酵母烟酸酯磷酸核糖基转移酶；nma1：酿酒酵母烟酸单核苷酸腺苷转移酶1；nma2：酿酒酵母烟酸单核苷酸腺苷转移酶2；qns1：酿酒酵母NAD合成酶(QNS1)。
发明详述
本发明是基于这样的发现，即可以使用编码酵母NAD补救途径中的植物功能酶的DNA序列获得转基因植物，所述转基因植物比不包含这些DNA序列的植物对胁迫特别是对非生物胁迫的抗性更强。与对照植物相比，转基因植物在胁迫条件下也表现出活性氧(ROS)水平显著降低和维持高水平的NADH。
因此在本发明的一个实施方案中，提供了获得具有增加的胁迫抗性的植物的方法，所述方法包括步骤：
-将如这里描述的胁迫抗性嵌合基因引入植物细胞中以获得含有这种胁迫抗性嵌合基因的细胞；
-再生这些含有胁迫抗性嵌合基因的细胞以获得含有胁迫抗性嵌合基因的植物群，和
-从这些植物群中选择植物，所述植物与类似的非转基因植物相比在胁迫条件下表现出增加的胁迫抗性和/或减少的ROS水平和/或维持高水平的NADH。
因此所述胁迫抗性嵌合基因包含有效连接到DNA区的可在植物中表达的启动子和参与转录终止和聚腺甘酸化的3’区，所述DNA区编码烟酰胺腺嘌呤二核苷酸补救合成途径中的植物功能酶，所述酶选自烟酰胺酶、烟酸酯磷酸核糖基转移酶、烟酸单核苷酸腺苷转移酶或烟酰胺腺嘌呤二核苷酸合成酶。
在这里使用“烟酰胺腺嘌呤二核苷酸补救合成途径中的植物功能酶”是指当连接到适当的控制元件(如可在植物中表达的启动子和终止子区)并将其引入到植物中时，能被转录和翻译产生在植物细胞中有功能的NAD补救合成途径中的酶。所包括的NAD补救合成途径中的酶(和编码基因)不仅从植物来源中获得，还从酵母(酿酒酵母)或其他酵母或真菌中获得。认为后一种来源的蛋白质甚至更适用于本发明的方法，因为这些蛋白质较少可能受到酶反馈调节等，而相似的植物来源的酶则容易受酶的反馈调节。
参与NAD补救合成途径的酶包括如下：
-烟酰胺酶(EC 3.5.1.19)催化烟酰胺上的酰胺基团水解，从而释放出烟酸和NH3。该酶又称为烟酰胺脱氨酶、烟酰胺酰胺酶、YNDase或烟酰胺酰胺水解酶。
-烟酸酯磷酸核糖基转移酶(EC 2.4.2.11)又称为烟酸核苷酸酶、烟酸单核苷酸糖苷水解酶、烟酸单核苷酸焦磷酸化酶、烟酸磷酸核糖基转移酶，其催化下面的反应：
烟酸酯-D-核苷酸+二磷酸＝烟酸酯+5-磷酸-α-D-核糖1-二磷酸。
-烟酸酯-核苷酸腺苷转移酶(EC 2.7.7.18)又称为脱酰胺NAD+焦磷酸化酶、烟酸酯单核苷酸腺苷转移酶、脱酰胺烟酰胺腺嘌呤二核苷酸焦磷酸化酶、NaMT-ATase、烟酸单核苷酸腺苷转移酶，其催化下面的反应：
ATP+烟酸酯核苷酸＝二磷酸+脱酰胺NAD+
NAD合酶(EC 6.3.1.5)又称为NAD合成酶、NAD+合酶、烟酰胺腺嘌呤二核苷酸合成酶、二磷酸吡啶核苷酸合成酶，其催化下面的反应：
脱酰胺NAD++ATP+NH3＝AMP+二磷酸+NAD+
在本发明的一个实施方案中，编码NAD补救合成途径中不同的酶的编码区包含一段核苷酸序列，例如，SEQ ID No 1、SEQ ID No 3、SEQ IDNo 5、SEQ ID No 7或SEQ ID No 9的核苷酸序列，所述核苷酸序列编码蛋白质具有的氨基酸序列如SEQ ID No 2、SEQ ID No 4、SEQ ID No 6、SEQ ID No 8或SEQ ID No 10所列。
但是，应该明确的是，也可以使用这些核苷酸序列的变体，包括其插入、缺失和取代，达到同样的效果。同样地，可以使用来自除了酿酒酵母外其他物种的所提到的核苷酸序列的同源物。这些核苷酸序列包括但不限于植物来源的核苷酸序列，编码具有相同氨基酸序列的蛋白质的核苷酸序列，以及这些核苷酸序列的变体。后者的实例是编码带有SEQ ID No 12、14、16、18、20、22或24中列出的氨基酸序列的蛋白质的核苷酸序列，例如SEQ ID No 11、13、15、17、19、21或23的核苷酸序列。
所述核苷酸序列的变体与鉴定的编码NAD补救合成途径中酶的核苷酸序列(如序列表中鉴定的那些)具有序列同一性，其优选至少为约80％，或85％、或90％或95％。优选地，这些变体能编码与NAD补救途径中的酶具有相同酶活性的有功能的蛋白质。为了本发明的目的，用百分比表示的两个相关核苷酸或氨基酸序列的“序列同一性”指两个最佳比对序列中相同残基位置的数目(x100)除以所有比较残基位置的数目。空位即比对中残基在一个序列中存在而在另一个序列中没有的位置，被认为是没有相同残基的位置。两个序列的比对用Needleman和Wunsch算法(Needleman和Wunsch 1970)执行。上述计算机辅助的序列比对可以用标准软件程序如GAP方便地执行，所述GAP为Wisconsin Package Version 10.1(GeneticsComputer Group，Madision，Wisconsin，USA)的部分，使用带有空位产生罚分为50和空位延伸罚分为3的缺省得分矩阵。
如Hunt等人所述(见上)，可以通过基因组数据芯片(in silico)分析鉴定编码来自酵母NAD补救途径的酶的核苷酸序列或编码来自酵母之外生物体的酶的核苷酸序列的同源核苷酸序列。
同源核苷酸序列也可以使用编码NAD补救合成途径中酶的鉴定的核苷酸序列(如在序列表中鉴定的核苷酸序列)作为探针，在严格条件下通过杂交得以鉴定和分离。
如这里所用“严格杂交条件”意指杂交通常发生在探针和目标序列之间的同一性至少为95％，并优选至少97％时。严格杂交条件的实例是在含有50％甲醛、5×SSC(150mM NaCl、15mM柠檬酸三钠)、50mM磷酸钠(pH 7.6)、5×Denhardt杂交溶液、10％硫酸葡聚糖和20μg/ml变性剪切的载体DNA(如鲑鱼精子DNA)的溶液中孵育过夜，然后用0.1×SSC在大约65℃洗涤杂交支持物，优选洗两次每次约10分钟。其他的杂交和洗涤条件是众所周知的，并且示例于Sambrook等人，Molecular Cloning：ALaboratory Manual，第二版，Cold Spring Harbor，NY(1989)，特别是第11章。
这些变体序列也可以通过DNA扩增技术获得，其中使用对于编码NAD补救途径中的酶的基因具有特异性的寡核苷酸作为引物，例如但不限于包含约20至约50个连续核苷酸的寡核苷酸，所述连续核苷酸选自SEQID No 1、3、5、7、9、11、13、15、17、19、21、23的核苷酸序列或它们的互补序列。
可以使用本发明的方法获得耐受不同种类胁迫诱导条件特别是非生物胁迫条件的植物，所述非生物胁迫条件包括浸没、高光照条件、高UV辐射水平、增加的过氧化氢水平、干旱条件、高温或低温、增加的盐度条件。本发明的方法也可以用来降低生长在不利条件下特别是非生物胁迫条件下的植物细胞中ROS的水平，所述非生物胁迫条件包括浸没、高光照条件、高UV辐射水平、增加的过氧化氢水平、干旱条件、高温或低温、增加的盐度条件等。可以用在本领域内公知的方法(包括在实施例3中所述的方法)测定ROS水平或NADH水平。
使用这里所述方法可以获得植物，其中在非胁迫条件下(例如但不限于低光照条件下)其ROS的水平等于或低于对照植物。在非胁迫条件下，这些植物中的ROS水平可以是在低光照条件下对照植物水平的50％至100％，尤其在约60％至约85％之间。在胁迫条件下，这些植物中的ROS水平是胁迫条件下对照植物的ROS水平的大约50％-80％，相当于非胁迫条件下对照植物中的ROS水平的大约60％-80％。同样地，在非胁迫条件下，例如但不限于低光照条件下，这些植物的NADH水平等于或高于对照植物中的水平。在非胁迫条件下，这些植物中的NADH水平是在低光照条件下的对照植物中NADH水平的100％-160％之间，尤其在大约120％至大约140％之间。在胁迫条件下这些植物中的NADH水平是胁迫条件下对照植物中NADH水平的大约200％-300％，相当于非胁迫条件下对照植物中ROS水平的大约100％-160％。
获得转基因植物的方法不是本发明的关键，任何转化方法和适合具体植物物种的再生方法都可以使用。这些方法是本领域内众所周知的，其包括农杆菌介导的转化、基因枪递送、显微注射、完整细胞的电穿孔、聚乙二醇介导的原生质体转化、原生质体电穿孔、脂质体介导的转化、硅须介导的转化(silicon-whiskers mediated transformation)等。然后，用这种方式得到的转化细胞可以再生为成熟的有繁殖能力的植物。
获得的转化植物可以用于传统的育种方案以产生更多的具有相同特征的转化植物，或将本发明的嵌合基因引入相同或相关植物物种的其它品种或杂交植物中。从转化植物中得到的种子含有作为稳定的基因组插入片段的本发明的嵌合基因，并且也被包含在本发明中。
很清楚，这里描述的不同胁迫抗性嵌合基因含有编码NAD补救途径中不同酶的DNA区域，这些嵌合基因可以在一个植物细胞或植物中组合以进一步提高含有所述嵌合基因的植物的胁迫耐受性。因此，在本发明的一个实施方案中，提供的植物细胞和植物含有至少两个胁迫抗性嵌合基因，每一嵌合基因含有不同的编码区。
根据本发明的转基因植物细胞和植物系还可以含有能减少如WO00/04173和PCT/EP2004/003995所述的内源PARP和/或PARG基因表达的嵌合基因。可以例如通过将本发明的转基因植物系与含有使PARP和/或PARG基因表达减少的嵌合基因的转基因植物杂交引入更多的嵌合基因。也可以通过将本发明的嵌合基因引入或转化到含有使PARP或PARG基因表达减少的嵌合基因的转基因植物细胞获得转基因植物细胞或植物系，反之亦然。
为了本发明的目的，启动子是可在植物中表达的启动子。这里所用术语“可在植物中表达的启动子”意指能在植物细胞中控制(起始)转录的DNA序列。这包括植物起源的任何启动子，也包括非植物起源的但能在植物细胞中指导转录的任何启动子，即病毒或细菌起源的某些启动子，如CaMV35S(Harpster等人，1988 Mol.Gen.Genet.212，182-190)、地下三叶草病毒启动子No 4或No 7(WO9606932)、或T-DNA基因启动子，还包括组织特异性或器官特异性启动子，其包括但不限于种子特异性启动子(例如WO89/03887)、器官原基特异性启动子(An等人，1996，The Plant Cell 8，15-30)、茎特异性启动子(Keller等人，1988，EMBO J.7，3625-3633)、叶特异性启动子(Hudspeth等人，1989，Plant Mol Biol 12，579-589)、叶肉特异性启动子(如光诱导的核酮糖二磷酸羧化酶加氧酶启动子)、根特异性启动子(Keller等人，1989，Genes Devel.3，1639-1646)、块茎特异性启动子(Keil等人，1989，EMBO J.8，1323-1330)、维管组织特异性启动子(Peleman等人，1989，Gene 84，359-369)、雄蕊选择性启动子(WO 89/10396、WO 92/13956)、裂开区特异性启动子(WO 97/13865)等等。
本发明的嵌合基因也可以装备在植物中有功能的核定位信号(“NLS”)，如SV40NLS，所述NLS有效地连接到编码NAD补救途径中的酶的DNA区。
读到这篇文献，本领域的技术人员将会立即认识到，只要获得其中编码NAD补救途径中的酶的内源基因更加活跃或以更高水平表达的植物的天然变体可以获得增加胁迫抗性的类似效果。这样的变体植物可以这样得到：对植物群进行诱变，例如但不限于EMS诱变，然后再筛选NAD补救途径中任何一个酶或其组合的活性增加的植物。
同样立即显而易见的是，可以筛选对前面提到的一般性胁迫条件(特别是非生物胁迫条件)增加耐受性的很多不同的品种或栽培种，然后将对胁迫条件增加的耐受性与存在编码NAD补救途径中酶的任何内源基因的特定等位基因相关联。如果这些物种是性亲和的，可以通过杂交将这样的等位基因引入目的植物中；或者使用这里描述的常规方法(包括杂交技术或PCR扩增技术)对其鉴定并用重组DNA技术引入植物中。用育种技术引入特别期望的等位基因后可以接着用目的等位基因特异性的分子标记检测。
认为这里描述的方法和手段适合所有植物细胞和植物，同时包括双子叶和单子叶植物细胞，并且植物包括但不限于棉花、芸苔属植物、油料种子油菜、小麦、谷物或玉米、大麦、向日葵、稻、燕麦、甘蔗、大豆、蔬菜(包括菊苣、莴苣、番茄)、烟草、马铃薯、甜菜、木瓜、菠萝、芒果、拟南芥，也包括在园艺、花卉或林业中使用的植物。
在这里使用的“包含”应阐释为说明陈述特征、整体、步骤或成分的存在，但是并不排除存在或增加一种或多种特征、整体、步骤或成分，或它们组成的组。因此，例如，包含核苷酸或氨基酸序列的核酸或蛋白质可以比实际引述的核酸或蛋白质包含更多的核苷酸或氨基酸，即所述实际引述的核酸或蛋白质包含在更大的核酸和蛋白质中。含有确定结构或功能的DNA区的嵌合基因可以包含额外的DNA区等。
具体实施方式
以下非限制性实施例描述了能增加植物细胞和植物胁迫抗性的嵌合基因的构建和这些基因的使用。
除非在实施例中另有说明，所有重组DNA技术都是根据在Sambrook等人(1989)Molecular Cloning：A Laboratory Manual，第二版，ColdSpring Harbor Laboratory Press，NY和Ausubel等人(1994)CurrentProtocols in Molecular Biology，Current Protocols，USA第1、2卷中描述的标准方案实施。植物分子工作的标准材料和方法于R.D.D.Croy编著的由BIOS Scientific Publications Ltd(UK)和Blackwell ScientificPublications，UK联合出版的Plant Molecular Biology Labfax(1993)中得到描述。标准分子生物学技术的其它参考书包括Sambrook和Russell(2001)Molecular Cloning：A Laboratory Manual，第三版，Cold Spring HarborLaboratory Press，NY，Brown所著卷I和II(1998)Molecular BiologyLabFax，第二版，Academic Press(UK)。聚合酶链式反应的标准材料和方法见Dieffenbach和Dveksler(1995)PCR Primer：A Laboratory Manual，Cold Spring Harbor Laboratory Press，以及McPherson等人(2000)PCR-Basics：From Background to Bench，第一版，Springer Verlag，Germany。
在所有的描述和实施例中，参考下面的序列：
SEQ ID No.1：酿酒酵母烟酰胺酶(PNC1)的核苷酸序列。
SEQ ID No.2：酿酒酵母烟酰胺酶(PNC1)的氨基酸序列。
SEQ ID No.3：酿酒酵母烟酸酯磷酸核糖基转移酶(NPT1)的核苷酸序列(互补)。
SEQ ID No.4：酿酒酵母烟酸酯磷酸核糖基转移酶(NPT1)的氨基酸序列。
SEQ ID No.5：酿酒酵母烟酸单核苷酸腺苷转移酶1(NMA1)的核苷酸序列。
SEQ ID No.6：酿酒酵母烟酸单核苷酸腺苷转移酶1(NMA1)的氨基酸序列。
SEQ ID No.7：酿酒酵母烟酸单核苷酸腺苷转移酶2(NMA2)的核苷酸序列。
SEQ ID No.8：酿酒酵母的烟酸单核苷酸腺苷转移酶2(NMA2)的氨基酸序列。
SEQ ID No.9：酿酒酵母NAD合成酶(QNS1)的核苷酸序列。
SEQ ID No.10：酿酒酵母NAD合成酶(QNS1)的氨基酸序列。
SEQ ID No.11：拟南芥烟酰胺酶(同工型1)的核苷酸序列。
SEQ ID No.12：拟南芥烟酰胺酶(同工型1)的氨基酸序列。
SEQ ID No.13：拟南芥烟酰胺酶(同工型2)的核苷酸序列。
SEQ ID No.14：拟南芥烟酰胺酶(同工型2)的氨基酸序列。
SEQ ID No.15：拟南芥烟酰胺酶(同工型3)的核苷酸序列。
SEQ ID No.16：拟南芥烟酰胺酶(同工型3)的氨基酸序列。
SEQ ID No.17：拟南芥烟酸酯磷酸核糖基转移酶(同工型1)的核苷酸序列。
SEQ ID No.18：拟南芥烟酸酯磷酸核糖基转移酶(同工型1)的氨基酸序列。
SEQ ID No.19：拟南芥烟酸酯磷酸核糖基转移酶(同工型2)的核苷酸序列。
SEQ ID No.20：拟南芥烟酸酯磷酸核糖基转移酶(同工型2)的氨基酸序列。
SEQ ID No.21：拟南芥烟酸单核苷酸腺苷转移酶的核苷酸序列。
SEQ ID No.22：拟南芥烟酸单核苷酸腺苷转移酶的氨基酸序列。
SEQ ID No.23：拟南芥NAD合成酶的核苷酸序列。
SEQ ID No.24：拟南芥NAD合成酶的氨基酸序列。
SEQ ID No.25：T-DNA载体pTVE467的核苷酸序列。
SEQ ID No.26：T-DNA载体pTVE468的核苷酸序列。
SEQ ID No.27：T-DNA载体pTVE469的核苷酸序列。
SEQ ID No.28：T-DNA载体pTVE470的核苷酸序列。
SEQ ID No.29：T-DNA载体pTVE496的核苷酸序列。
SEQ ID No.30：T-DNA载体pTVE497的核苷酸序列。
SEQ ID No.31：T-DNA载体pTVE500的核苷酸序列。
SEQ ID No.32：T-DNA载体pTVE501的核苷酸序列。
SEQ ID No.33：T-DNA载体pTVE502的核苷酸序列。
SEQ ID No.34：T-DNA载体pTVE503的核苷酸序列。
实施例
实施例1：胁迫抗性基因的组装和引入到植物中。
pTVE467
为了增加植物的胁迫抗性，用常规技术构建的嵌合基因依次含有以下DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个碱基对的DNA片段；
-编码酿酒酵母烟酰胺酶的DNA片段(SEQ ID NO 1)；
-来自花椰菜花叶病毒35S转录物的3’端非翻译末端的片段(3’35S)。
可以将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE467(SEQID 25)。T-DNA载体pTVE467如图2所示。
T-DNA载体pTVE467含有下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1181 | 534(C) | PNC1编码区 |
| 1250 | 1191(C) | cab22前导序列 |
| 1781 | 1251(C) | P35S2启动子 |
| 2293 | 2082(C) | 3′g7转录终止信号 |
| 2866 | 2315(C) | bar编码区 |
| 4592 | 2867(C) | PSSuAra启动子 |
| 4760 | 4784 | T-DNA左边界 |
| 6352 | 5352(C) | Sm/Sp抗性基因 |
| 6875 | 10645 | pVS1复制起点 |
| 10646 | 11709 | ColE1复制起点 |
pTVE468
构建与pTVE467中所示类似的嵌合基因，其中烟酰胺酶装备了标准核定位信号。因此，该嵌合基因包含以下有效连接的DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个核苷酸的DNA片段；
-编码含有核定位信号的肽的约20个核苷酸的DNA片段；
-编码酿酒酵母烟酰胺酶的DNA片段(SEQ ID NO 1)，由此将核定位信号融合在阅读框内；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
这一嵌合基因和能提供对除草剂膦丝菌素抗性的选择性标记基因一起被引入到T-DNA载体的左边界和右边界序列之间，产生pTVE468(SEQ ID26)。T-DNA载体pTVE468如图3所示。
T-DNA载体pTVE468包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1169 | 534(C) | PNC1编码区 |
| 1187 | 1167(C) | 核定位信号 |
| 1268 | 1209(C) | cab22前导序列 |
| 1799 | 1269(C) | P35S2启动子 |
| 2311 | 2100(C) | 3′g7转录终止信号 |
| 2884 | 2333(C) | bar编码区 |
| 4610 | 2885(C) | PSSuAra启动子 |
| 4778 | 4802 | T-DNA左边界 |
| 6370 | 5370(C) | Sm/Sp抗性基因 |
| 6893 | 10663 | pVS1复制起点 |
| 10664 | 11727 | ColE1复制起点 |
pTVE469
为了增加植物的胁迫抗性，用常规技术构建的嵌合基因依次含有以下DNA片段：
-来自花椰菜花叶病毒的启动子(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个碱基对的DNA片段；
-编码酿酒酵母烟酸酯磷酸核糖基转移酶(NPT1；SEQ ID NO 3)的DNA片段；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
将这一嵌合基因和能提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE469(SEQ ID27)。T-DNA载体pTVE469如图4所示。
T-DNA载体pTVE469包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1765 | 534(C) | NPT1编码区 |
| 1832 | 1773(C) | cab22前导序列 |
| 2363 | 1833(C) | P35S2启动子 |
| 2875 | 2664(C) | 3′g7转录终止信号 |
| 3448 | 2897(C) | bar编码区 |
| 5175 | 3449(C) | PSSuAra启动子 |
| 5342 | 5366 | T-DNA左边界 |
| 6934 | 5934(C) | Sm/Sp抗性基因 |
| 7457 | 11227 | pVS1复制起点 |
| 11228 | 12291 | ColE1复制起点 |
pTVE470
构建与pTVE469所示类似的嵌合基因，其中酵母烟酸酯磷酸核糖基转移酶装备了标准核定位信号。因此，这个嵌合基因包含以下有效连接的DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个核苷酸的DNA片段；
-编码含有核定位信号的肽的约20个核苷酸的DNA片段；
-编码酿酒酵母烟酸酯磷酸核糖基转移酶(NPT1；SEQ ID NO 3)的DNA片段，由此将核定位信号融合在阅读框内；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE470(SEQ ID28)。T-DNA载体pTVE470如图5所示。
T-DNA载体pTVE470包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1787 | 534(C) | NPT1编码区 |
| 1775 | 1755(C) | SV40核定位信号 |
| 1853 | 1794(C) | cab22前导序列 |
| 2384 | 1854(C) | P35S2启动子 |
| 2896 | 2685(C) | 3′g7转录终止信号 |
| 3469 | 2918(C) | bar编码区 |
| 5195 | 3470(C) | PSSuAra启动子 |
| 5363 | 5387 | T-DNA左边界 |
| 6955 | 5955(C) | Sm/Sp抗性基因 |
| 7478 | 11248 | pVS1复制起点 |
| 11249 | 12312 | ColE1复制起点 |
pTVE496
为了增加植物的胁迫抗性，用常规技术构建成的嵌合基因依次含有以下DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个碱基对的DNA片段；
-编码酿酒酵母烟酸单核苷酸腺苷转移酶(NMA1；SEQ ID NO 5)的DNA片段；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE496(SEQ ID29)。T-DNA载体pTVE496如图6所示。
T-DNA载体pTVE496包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1739 | 534(C) | NMA1编码区 |
| 1805 | 1746(C) | cab22前导序列 |
| 2336 | 1806(C) | P35S2启动子 |
| 2848 | 2637(C) | 3′g7转录终止信号 |
| 3421 | 2870(C) | bar编码区 |
| 5147 | 3422(C) | PSSuAra启动子 |
| 5315 | 5339 | T-DNA左边界 |
| 6907 | 5907(C) | Sm/Sp抗性基因 |
| 7430 | 11200 | pVS1复制起点 |
| 11201 | 12264 | ColE1复制起点 |
pTVE497
构建与pTVE496所示类似的嵌合基因，其中酵母烟酸单核苷酸腺苷转移酶装备了标准核定位信号。因此，这个嵌合基因包含以下有效连接的DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个核苷酸的DNA片段；
-编码含有核定位信号的肽的约20个核苷酸的DNA片段；
-编码酿酒酵母烟酸单核苷酸腺苷转移酶(NMA1；SEQ ID NO 5)的DNA片段，由此将核定位信号融合在阅读框内；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE497(SEQ ID30)。T-DNA载体pTVE497如图7所示。
T-DNA载体pTVE497包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1757 | 534(C) | NMA1编码区 |
| 1748 | 1731(C) | SV40核定位信号 |
| 1823 | 1764(C) | cab22前导序列 |
| 2354 | 1824(C) | P35S2启动子 |
| 2866 | 2655(C) | 3′g7转录终止信号 |
| 3439 | 2888(C) | bar编码区 |
| 5165 | 3440(C) | PSSuAra启动子 |
| 5333 | 5357 | T-DNA左边界 |
| 6925 | 5925(C) | Sm/Sp抗性基因 |
| 7448 | 11218 | pVS1复制起点 |
| 11219 | 12282 | ColE1复制起点 |
pTVE500
为了增加植物的胁迫抗性，用常规技术构建成的嵌合基因依次含有以下DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个碱基对的DNA片段；
-编码酿酒酵母中的烟酸单核苷酸腺苷转移酶2(NMA2；SEQ ID NO7)的DNA片段；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE500(SEQ ID31)。T-DNA载体pTVE500如图8所示。
T-DNA载体pTVE500包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1721 | 534(C) | NMA2编码区 |
| 1787 | 1728(C) | cab22前导序列 |
| 2318 | 1788(C) | P35S2启动子 |
| 2830 | 2619(C) | 3′g7转录终止信号 |
| 3403 | 2852(C) | bar编码区 |
| 5129 | 3404(C) | PSSuAra启动子 |
| 5297 | 5321 | T-DNA左边界 |
| 6889 | 5889(C) | Sm/Sp抗性基因 |
| 7412 | 11182 | pVS1复制起点 |
| 11183 | 12246 | ColE1复制起点 |
pTVE501
构建与pTVE500所示类似的嵌合基因，其中酿酒酵母烟酸单核苷酸腺苷转移酶2装备了标准核定位信号。因此，这个嵌合基因包含以下有效连接的DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个核苷酸的DNA片段；
-编码含有核定位信号的肽的约20个核苷酸的DNA片段；
-编码酿酒酵母烟酸单核苷酸腺苷转移酶2(NMA2；SEQ ID NO 7)的DNA片段，由此将核定位信号融合在阅读框内；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE501(SEQ ID32)。T-DNA载体pTVE501如图9所示。
T-DNA载体pTVE501包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 1739 | 534(C) | NMA2编码区 |
| 1733 | 1713(C) | SV40核定位信号 |
| 1805 | 1746(C) | cab22前导序列 |
| 2336 | 1806(C) | P35S2启动子 |
| 2848 | 2637(C) | 3′g7转录终止信号 |
| 3421 | 2870(C) | bar编码区 |
| 5165 | 3440(C) | PSSuAra启动子 |
| 5315 | 5339 | T-DNA左边界 |
| 6907 | 5907(C) | Sm/Sp抗性基因 |
| 7430 | 11200 | pVS1复制起点 |
| 11201 | 12264 | ColE1复制起点 |
pTVE502
为了增加植物的胁迫抗性，用常规技术构建的嵌合基因依次含有以下DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个碱基对的DNA片段；
-编码酿酒酵母中的NAD合酶(QNS1；SEQ ID NO 9)的DNA片段；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE502(SEQ ID33)。T-DNA载体pTVE502如图10所示。
T-DNA载体pTVE502包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 2678 | 534(C) | QNS1编码区 |
| 2744 | 2685(C) | cab22前导序列 |
| 3275 | 2745(C) | P35S2启动子 |
| 3787 | 3576(C) | 3′g7转录终止信号 |
| 4360 | 3809(C) | bar编码区 |
| 6086 | 4361(C) | PSSuAra启动子 |
| 6254 | 6278 | T-DNA左边界 |
| 7846 | 6846(C) | Sm/Sp抗性基因 |
| 8369 | 12139 | pVS1复制起点 |
| 12140 | 13203 | ColE1复制起点 |
pTVE503
构建与pTVE502所示类似的嵌合基因，其中酿酒酵母中NAD合酶装备了标准核定位信号。因此，这个嵌合基因包含以下有效连接的DNA片段：
-来自花椰菜花叶病毒的启动子区域(CaMV35S)；
-对应Cab22L非翻译前导序列的约60个核苷酸的DNA片段；
-编码含有核定位信号的肽的约20个核苷酸的DNA片段；
-编码酿酒酵母NAD合酶(QNS1；SEQ ID NO 9)的DNA片段，由此将核定位信号融合在阅读框内；
-来自花椰菜花叶病毒35S转录子的3’端非翻译末端的片段(3’35S)。
将这一嵌合基因和提供对除草剂膦丝菌素抗性的选择性标记基因一起引入到T-DNA载体的左边界和右边界序列之间，产生pTVE503(SEQ ID34)。T-DNA载体pTVE503如图11所示。
T-DNA载体pTVE503包含下列分子特征：
(C)表示互补链。
| 起始(nt) | 终止(nt) | |
| 198 | 222 | RB：T-DNA右边界 |
| 521 | 300(C) | 3′35S：转录终止信号 |
| 2699 | 534(C) | QNS1编码区 |
| 2690 | 2670(C) | SV40核定位信号 |
| 2765 | 2706(C) | cab22前导序列 |
| 3296 | 2766(C) | P35S2启动子 |
| 3808 | 3597(C) | 3′g7转录终止信号 |
| 4381 | 3830(C) | bar编码区 |
| 6107 | 4382(C) | PSSuAra启动子 |
| 6275 | 6299 | T-DNA左边界 |
| 7867 | 6867(C) | Sm/Sp抗性基因 |
| 8390 | 12610 | pVS1复制起点 |
| 12161 | 13224 | ColE1复制起点 |
用常规方法将T-DNA载体引入到含有辅助Ti质粒的农杆菌菌株中。用如本领域所述农杆菌介导的转化方法将嵌合基因引入到拟南芥植物中。
实施例2：对含有实施例1所述嵌合基因的转基因拟南芥系的分析
如在实施例1所述获得的表达酵母NAD补救途径基因的转基因拟南芥系(T1代)的种子在含有15mg/L膦丝菌素(PPT)的培养基上萌发并生长。拟南芥cv Col-0作为对照。
所有的植物都用高光照胁迫处理。将在每秒30μEinstein/m2生长的两周龄的植物转移到每秒250μEinstein/m2(高光照)中处理6小时；接着在黑暗中8小时后；再用高光照8小时。
这种处理后，检测所有植物系中的NADH含量和超氧化物自由基的含量，并与在低光照条件下转基因和对照植物系中相同化合物的测量相比较。结果概括于表1。
在高光照条件下转基因植物比对照植物表现出更高水平的NADH含量，并且在高光照条件下转基因植物比对照植物产生更少的活性氧。其中在装备或未装备核定位信号的编码NAD补救途径酶的构建体之间没有观察到差异。
还对转基因植物系对高光照胁迫条件的耐受性进行了表型计分。为此，将植物在体外低光照条件下(每秒30μEinstein/m2)生长两周而后转到高光照条件(每秒250μEinstein/m2；16小时光照-8小时黑暗)3天。在高光照处理后，将植物转回到低光照条件下，并在表型计分前再生长3天。
对照植物矮小，并且已经开始开花(胁迫诱导)；而含有如实施例1中所述嵌合基因的转基因植物系则比对照植物高大并且仅开始抽薹(bolt)。
表1.过表达如实施例1所述嵌合酵母基因的转基因拟南芥系的高光照耐受性。
平均值的标准误差＜10％
实施例3：NADH含量和超氧化物含量的检测方案
用水溶性四氮唑盐定量细胞内NAD(P)H
参考文献
Jun Nakamura，Shoji Asakura，Susan D.Hester，Gilbert de Murcia，Keith W.Caldecott和James A.Swenberg(2003)Quantitation ofintracellular NAD(P)H can monitor an imbalance of DNA single strandbreak repair in base excision repair deficient cells in real time.NucleicAcids Research 31(17)，e104.
植物材料
最常用的植物材料
-体外生长14-18天但未开花的拟南芥芽
-油料种子油菜的下胚轴外植体
细胞计数试剂盒-8(CCK-8)
Sopachem n.v./比利时
72A，Avenue du Laarbeeklaan-1090Brussels
比利时
内含物：
5mL瓶中含有5mMol/L的WST-8(四氮唑盐)、0.2mMol/L的1-甲氧基PMS、150mMol/L NaCl
反应溶液：
-10ml 25mM磷酸钾缓冲液pH7.4
-0.5ml CCK-8
-0.1mM 1-甲氧基-5-甲基吩嗪甲基硫酸盐(＝1-甲氧基吩嗪硫酸二甲酯)：1μL/mL的100mM储液(分子量＝336.4；2.973ml水中溶100mg)
-1滴吐温20/25ml
操作
-收集植物材料并置于25mM的磷酸钾缓冲液(pH7.4)中。
如150个油料种子油菜的下胚轴外植体
1克拟南芥的芽(不带根)
-用反应溶液替换缓冲液
1克拟南芥苗加15ml
150个油料种子油菜下胚轴外植体加15ml
-在黑暗中于26℃孵育0.5小时(接着反应)
-在450nm测量反应溶液的吸光度
通过定量XTT的减少来测量超氧化物的产生
参考文献
De Block，M.，De Brouwer，D.(2002)A simple and robust in vitro assayto quantify the vigour of oilseed rape lines and hybrids.Plant Physiol.Biochem.40，845-852
A.欧洲油菜(BRASSICA NAPUS)
培养基和反应缓冲液
播种培养基(培养基201)：
半浓缩的Murashige和Skoog盐
2％的蔗糖
pH 5.8
0.6％琼脂(Difco Bacto琼脂)
250mg/l triacillin
愈伤组织诱导培养基A2S3：
MS培养基、0.5g/l Mes(pH5.8)、3％蔗糖、40mg/l硫酸腺嘌呤、0.5％琼脂糖、1mg/l 2，4-D、0.25mg/l NAA、1mg/l BAP、250mg/ltriacillin。
反应缓冲液：
25mM磷酸钾缓冲液pH8，
1mM 3’-{1-[苯基氨-羰基]-3，4-四氮唑}-双(4-甲氧基-6-硝基)钠＝XTT(BioVectra，Canada)(分子量674.53)
通过小心温热溶液(±37℃)溶解XTT(用前冷却到室温)
每25ml缓冲液中加1滴吐温20
种子消毒-种子预萌发-幼苗生长
种子在70％的乙醇中浸泡2分钟，然后用含有0.1％吐温20的次氯酸钠溶液(含有约6％的活性氯)表面消毒15分钟。最后，用1升无菌自来水冲洗种子。将种子在无菌自来水中孵育至少1小时(让可能抑制萌发的成分从种子扩散)。
将种子放在含有50毫升无菌自来水(+250mg/l triacillin)的250ml锥形瓶(erlenmeyer flask)中。摇动约20小时。
将长出根的种子从含有约125ml播种培养基的Duchefa(每个容器10粒种子，不要太多以减少由于污染带来的种子损失)放到实验室通风容器中。这些种子在±24℃和每天16小时光照(每秒10-
μEinstein/m2)条件下萌发。
附：为了计算，必须播种的种子数目：每个幼苗5个下胚轴片段下胚轴外植体的预培养和胁迫的诱导
-播种12-14天后，将下胚轴切为约7-10mm片段。
-下胚轴外植体(25个下胚轴/Optilux培养皿，Falcon S1005，丹麦)在A2S2培养基中在25℃(于每秒10-30μEinstein/m2)条件下培养5天。附：每个条件使用150个下胚轴外植体。
-胁迫诱导：
将下胚轴外植体转移到分别含有0、25和50mg/l乙酰水杨酸的A2S3培养基中。
在25℃和每天16小时光照(每秒10-30μEinstein/m2)的条件下孵育约24小时。
XTT测定
-将150个下胚轴外植体转移到50ml Falcon管中。
-用反应缓冲液(无XTT)洗涤。
-加20ml反应缓冲液+XTT
(必须浸没外植体，而不要真空渗入)
-在黑暗中于26℃孵育。
-反应后，在470nm测量反应培养基的吸光度。
B.拟南芥
培养基和反应缓冲液
植物培养基：
半浓缩的Murashige和Skoog盐
B5维生素
1.5％蔗糖
pH 5.8
0.7％Difco琼脂
孵育培养基：
半浓缩的MS盐
1％蔗糖
0.5g/l MES pH5.8
每25ml培养基中加1滴吐温20
反应缓冲液：
25mM磷酸钾缓冲液pH8.0，
1mM 3’-{1-[苯基氨-羰基]-3，4-四氮唑}-二(4-甲氧基-6-硝基)钠＝XTT(BioVectra，Canada)(分子量674.53)
通过小心温热溶液(±37℃)溶解XTT(用前冷却到室温)
每25ml缓冲液中加1滴吐温20
拟南芥植物
-拟南芥株系：用于测试的对照株系(用来获得测试株系的母株系)
-拟南芥种子的消毒：
用70％乙醇处理2分钟
用每20ml溶液(含有6％活性氯)中加入1滴吐温20的溶液漂白10分钟
用无菌自来水冲洗5次
附：消毒是在2ml的Eppendorf管中进行。拟南芥种子沉到管底，使能够用1ml的自动移液器(pipetman)移去液体。
-种子的预萌发：
在直径为9cm的Optilux培养皿(Falcon)中含有12ml无菌自来水。低光照过夜到24小时。
-拟南芥植物的生长：
将种子播种在含有±125ml植物培养基的Falcon的内格(intergrid)组织培养皿(nr.3025)中：每格放1粒种子。植物抽薹前在24℃和16小时光照(每秒30μEinstein/m2)-8小时黑暗的条件下生长约18天。
附：实施测定需要1克的植物材料(不带根的芽)/株系/条件。1克芽对应于40-60株植物。
胁迫诱导
百草枯
-收获拟南芥芽(无根)
-将1克芽放在分别含有0、5和10μM百草枯的孵育培养基中(必须浸没芽，而不要真空渗透)。
孵育培养基：在Falcon的内格组织培养皿(nr.3025)中±150ml-在24℃黑暗中孵育±24小时以及每天16小时光照(每秒30-50μEinstein/m2)。
高光照
-将半数平皿转移到高光照(每秒250μEinstein/m2)并孵育4-20小时。
XTT测定
-从琼脂平皿中(高光照胁迫)或从液体孵育培养基中(百草枯胁迫)收获芽(无根)，并将其放在含反应缓冲液(无XTT)的50ml的Falcon管中。
-用含有XTT(15ml/g)的缓冲液替换反应缓冲液；
-必须浸没芽，而不要真空渗透；
-在黑暗中于26℃孵育；
-反应后，在470nm处检测反应培养基的吸光度(约1小时)。
实施例4：过表达酵母烟酰胺酶(Pnc1)基因增加拟南芥植物对臭氧的耐受
性
将嵌合载体pTVE467(实施例1)用于哥伦比亚生态型拟南芥的转化。用Southern-DNA和Northern-RNA印迹分析初始转化体。鉴定到一个转基因系带有单个拷贝的Pnc1转基因构建体并且具有高稳态水平的转基因全长Pnc1-mRNA(20pg/5μg总RNA)。
萌发6周之后，将含有单个拷贝转基因系的植物和作为对照的哥伦比亚野生型各100株植物在烟熏室内暴露于臭氧。在连续两天中分别用250、350、500ppb的臭氧浓度处理植物，每天5小时。处理之后，对全部植物肉眼筛选表现为坏死斑的臭氧损伤植物。结果概括于表2。在500ppb臭氧暴露处理中，几乎所有的植物表现为坏死斑，但在2个较低臭氧浓度的暴露处理中，转基因植物受伤的百分比显著减少。
另外，在增加的臭氧浓度下，确定所有转基因株系和野生型植物的生存力表现指数(PI)的演化。PI可以通过公式计算：PI＝(ABS/CS)×(TR/CS)×(ET/CS)(ABS＝由天线色素Chi＊吸收的光子流量；CS＝横断面；TR＝由反应中心捕获的并转化为氧化还原能的能量；ET＝进一步流向下游引起CO2固定的电子流量)。转基因株系的生存力表现指数PI随着臭氧浓度的增加显著地增加，而野生型植物的生存力表现指数随着臭氧浓度的增加保持恒定。这可以解释为在转基因株系中为对抗臭氧伤害产生的补偿性生理应答。
表2：过表达酵母烟酰胺酶(Pnc1)基因的拟南芥植物对臭氧的耐受性增加
| 250ppb O3 | 350ppb O3 | 500ppb O3 | |
| 野生型 | 45％＊ | 50％ | 100％ |
| Pnc1 | 20％ | 25％ | 100％ |
＊呈现坏死斑的植物的百分比
此外，还将对照植物，含有嵌合基因Pnc1的纯合子转基因植物种群以及杂合子转基因种群用臭氧烟熏处理，并计录了肉眼可见的伤害以及与未烟熏植物相比的多种生理应答。所述评估包括非调节荧光、调节荧光、叶绿素的测量以及新增重量的确定。
根据肉眼可见的伤害和生理应答，对每个种群定级表明臭氧影响的程度。评估越是负值，种群对臭氧的应答越敏感。
对照非转基因种群和杂合子转基因种群的累计得分为-13，而两个纯合子转基因种群的得分分别是-6和-2。因此，很明显，纯合子转基因种群在统计学上的表现显著优于对照植物。
序列表
<110>拜尔生物科学公司
<120>胁迫抗性植物
<130>BCS04-2005WO1
<150>EP 04077624.7
<151>2004-09-24
<150>US 60/628826
<151>2004-11-17
<160>34
<170>PatentIn version 3.0
<210>1
<211>651
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<213>酿酒酵母(Saccharomyces cerevisiae)
<400>1
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actgttccaa aaggtgagga attaatcaat cctatctcgg atttgatgca agatgctgat 120
agagactggc acaggattgt ggtcaccaga gattggcacc cttccagaca tatttcgttc 180
gcaaagaacc ataaagataa agaaccctat tcaacataca cctaccactc tccaaggcca 240
ggcgatgatt ccacgcaaga gggtattttg tggcccgtac actgtgtgaa aaacacctgg 300
ggtagtcaat tggttgacca aataatggac caagtggtca ctaagcatat taagattgtc 360
gacaagggtt tcttgactga ccgtgaatac tactccgcct tccacgacat ctggaacttc 420
cataagaccg acatgaacaa gtacttagaa aagcatcata cagacgaggt ttacattgtc 480
ggtgtagctt tggagtattg tgtcaaagcc accgccattt ccgctgcaga actaggttat 540
aagaccactg tcctgctgga ttacacaaga cccatcagcg atgatcccga agtcatcaat 600
aaggttaagg aagagttgaa ggcccacaac atcaatgtcg tggataaata a 651
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35 40 45
Thr Arg Asp Trp His Pro Ser Arg His Ile Ser Phe Ala Lys Asn His
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Lys Asp Lys Glu Pro Tyr Ser Thr Tyr Thr Tyr His Ser Pro Arg Pro
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100 105 110
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tttgatagcg tgattaccat ttacttctaa tagtttgata acgatgttta acggctcact 180
tttaacctgg ggttctgact tcttacgaaa atcattagta aagtttgtgc caataccgaa 240
tgtggctagc attccattct ctttagctgc atgggagtaa gttattgcct tttcgacgtt 300
caaagaatcg gaataacaga taatcttcga gaatttaggc aatttcaaca cgtcatggta 360
atggtgggaa atctttttgg tatactcaac tgggtctcca gaatcttgtc taacaccgac 420
gtaagcatca gaatatggtg gacggaatga ttttaaaaag tcatcagttc caaaagtatc 480
cgttaatgct aaaccagcat tttttgcacc aaaagtattg atccaacaat ccattgcatt 540
tttattggca tgcaaataat cttcactaat agaagcgact cccataaccc actcgtgagc 600
cacagtaccg attggcttga ctccatattt cttggcaaat aaaatatttg atgtgcctaa 660
taatagcgat ttgtttctgt ctgggttacc gttcacagct ttcatgattc cttgcataat 720
tagatcttga gccttcagag atctacgacg tcttgtacca aattcactga atctaatacc 780
attatcaaac aaagtttccg ccttcttctc agcttgttct aattggtttt cgtagtccca 840
gtcgatgtca acaaatttaa aatacgcttc tgatattagg gacagtaagg ggatctcata 900
aaggatagta tccttccaac taccactgac taaaattttc aatttgtagt gggtgggctt 960
gccctcgatt tcttctgaag tgaaggaaat ctgctcttca gggtgtagtt tgtaattaga 1020
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aatttcctct tctgtgaacc tcaaatttcc caaatacgaa aattgctctt tcaaccaatt 1140
aatggcttcc ttattgaagg tcaattggga cgacctgttg gtatatttat aagtaactgt 1200
aacatctgga aaattagtga agacagcagc atgcatcgta atcttgtaca tgtctgtgtc 1260
caaaagagac tttatcactg gttctgacat 1290
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Thr Tyr Lys Tyr Thr Asn Arg Ser Ser Gln Leu Thr Phe Asn Lys Glu
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Phe Thr Glu Glu Glu Ile Glu Tyr Leu Lys Gln Glu Ile Pro Tyr Leu
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Pro Ser Ala Tyr Ile Lys Tyr Ile Ser Ser Ser Asn Tyr Lys Leu His
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Pro Glu Glu Gln Ile Ser Phe Thr Ser Glu Glu Ile Glu Gly Lys Pro
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Thr His Tyr Lys Leu Lys Ile Leu Val Ser Gly Ser Trp Lys Asp Thr
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Ile Leu Tyr Glu Ile Pro Leu Leu Ser Leu Ile Ser Glu Ala Tyr Phe
130 135 140
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Ile Asn Thr Phe Gly Ala Lys Asn Ala Gly Leu Ala Leu Thr Asp Thr
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atggatccca caagagctcc ggatttcaaa ccgccatctg cagacgagga attgattcct 60
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ggtgttcaga aatatcagat tgctgattta gaagaagttc cacatggaat cgttcgtcaa 420
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catctaagaa tgtttgaaat ggctttagat gcaatctctg aacaaacaag gtttgaagtc 600
ataggtggat attactcccc tgttagtgat aactatcaaa agcaaggctt ggccccatcc 660
taccatagag tacgtatgtg tgaattggcc tgcgaaagaa cctcatcttg gttgatggtg 720
gatgcatggg agtcattgca accttcatac acaagaactg ccaaggtctt ggatcatttc 780
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gaatga 1206
<210>6
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Glu
<210>7
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<400>7
atggatccca ccaaagcacc cgattttaaa ccgccacagc caaatgaaga actacaacca 60
ccgccagatc caacacatac gataccaaaa tctggaccca tagttccata tgttttagct 120
gattataatt cttcgatcga tgctcctttc aatctcgaca tttacaaaac cctgtcgtca 180
aggaaaaaaa acgccaactc aagcaaccga atggaccata ttccattaaa tactagtgac 240
ttccagccac tatctcggga tgtatcatcg gaggaggaaa gtgaagggca atcgaatgga 300
attgacgcta ctctacagga tgttacgatg actgggaatt tgggggtact gaagagccaa 360
attgctgatt tggaagaagt tcctcacaca attgtaagac aagccagaac tattgaagat 420
tacgaatttc ctgtacacag attgacgaaa aagttacaag atcctgaaaa actgcctctg 480
atcatcgttg cttgtggatc attttctccc ataacatacc tacatttgag aatgtttgaa 540
atggctttag atgatatcaa tgagcaaacg cgttttgaag tggttggtgg ttatttttct 600
ccagtaagtg ataactatca aaagcgaggg ttagccccag cttatcatcg tgtccgcatg 660
tgcgaattag catgcgagcg gacatcatct tggttaatgg ttgatgcctg ggaatcttta 720
caatcaagtt atacaaggac agcaaaagtc ttggaccatt tcaatcatga aataaatatc 780
aagagaggtg gaatcatgac tgtagatggt gaaaaaatgg gcgtaaaaat catgttattg 840
gcaggcggtg atcttatcga atccatgggc gagcctcatg tgtgggctga ttcagacctg 900
caccatattt tgggtaatta tggatgtttg atcgtggaaa ggactggttc tgatgttagg 960
tccttcttgc tttcccatga tatcatgtat gaacacagaa gaaatatcct tattatcaaa 1020
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Pro Ile Val Pro Tyr Val Leu Ala Asp Tyr Asn Ser Ser Ile Asp Ala
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145 150 155 160
Ile Ile Val Ala Cys Gly Ser Phe Ser Pro Ile Thr Tyr Leu His Leu
165 170 175
Arg Met Phe Glu Met Ala Leu Asp Asp Ile Asn Glu G1n Thr Arg Phe
180 185 190
Glu Val Val Gly Gly Tyr Phe Ser Pro Val Ser Asp Asn Tyr Gln Lys
195 200 205
Arg Gly Leu Ala Pro Ala Tyr His Arg Val Arg Met Cys Glu Leu Ala
210 215 220
Cys Glu Arg Thr Ser Ser Trp Leu Met Val Asp Ala Trp Glu Ser Leu
225 230 235 240
Gln Ser Ser Tyr Thr Arg Thr Ala Lys Val Leu Asp His Phe Asn His
245 250 255
Glu Ile Asn Ile Lys Arg Gly Gly Ile Met Thr Val Asp Gly Glu Lys
260 265 270
Met Gly Val Lys Ile Met Leu Leu Ala Gly Gly Asp Leu Ile Glu Ser
275 280 285
Met Gly Glu Pro His Val Trp Ala Asp Ser Asp Leu His His Ile Leu
290 295 300
Gly Asn Tyr Gly Cys Leu Ile Val Glu Arg Thr Gly Ser Asp Val Arg
305 310 315 320
Ser Phe Leu Leu Ser His Asp Ile Met Tyr Glu His Arg Arg Asn Ile
325 330 335
Leu Ile Ile Lys Gln Leu Ile Tyr Asn Asp Ile Ser Ser Thr Lys Val
340 345 350
Arg Leu Phe Ile Arg Arg Gly Met Ser Val Gln Tyr Leu Leu Pro Asn
355 360 365
Ser Val Ile Arg Tyr Ile Gln Glu Tyr Asn Leu Tyr Ile Asn Gln Ser
370 375 380
Glu Pro Val Lys Gln Val Leu Asp Ser Lys Glu
385 390 395
<210>9
<211>2145
<212>DNA
<213>酿酒酵母
<400>9
atgtcacatc ttatcacttt agctacatgc aacttgaatc aatgggccct agattttgaa 60
ggtaatagag accgtatcct acagtccatt aagattgcca aagagagggg tgccaggtta 120
cgtgtcggcc cagaactgga aataactggc tacggatgtt tagatcattt tttagaaaat 180
gacgtttgcc ttcattcatg ggaaatgtat gctcaaatca ttaagaataa agaaacccat 240
ggattaatac ttgacattgg tatgcccgtt ctacacaaga atgttcgtta taattgtcgt 300
ttgttatcct tggatggtga gatattgttc ataagaccta agatttggtt agctaatgat 360
ggtaactata gggaaatgag atttttcaca ccttggatga aacctggcgt ggtggaggac 420
tttatccttc cacctgagat tcagaaagtt accggccaga gacttgtgcc atttggggac 480
gctgtgataa attcattgga tacatgcatt ggtacagaaa cttgtgaaga attgtttaca 540
cctcaatccc cccacatcgc catgtcttta gatggtgtgg aaatcatgac aaactcatct 600
ggttctcatc atgaactgcg taagttaaat aaaaggttag acctaatttt aaatgccact 660
aaacgttgtg gtggtgttta cttgtatgca aatcaaagag gttgtgatgg tgacagatta 720
tattatgatg gctgtgcact aattgccatc aatggtacaa ttgtagccca aggttcacaa 780
ttttcgctag atgatgtgga agtagttact gctactgtgg acctagaaga ggtgaggagt 840
tatcgtgcag ctgtcatgtc tcgtggccta caagcctcct tggcagaaat aaagttcaag 900
cgtattgata ttcctgtaga attggcttta atgacctcca gatttgatcc tacagtgtgt 960
ccaacaaaag tccgcgagcc tttctatcac tctcctgagg aagaaattgc actgggacct 1020
gcttgctgga tgtgggatta tttaagacgt tgtaacggaa cagggttttt ccttccctta 1080
tctgggggca ttgactcttg tgcaactgca atgattgtcc actctatgtg ccgtttagtg 1140
accgacgctg ctcaaaatgg aaatgagcaa gttatcaaag acgttcgtaa gataacacgt 1200
agcggcgatg attggattcc agacagtcca caggatctag cctcaaaaat atttcactcc 1260
tgtttcatgg gtacggaaaa ttcatccaag gagacaagaa acagagcaaa ggacctttcc 1320
aatgcaattg gatcttacca cgtggattta aagatggact cattggtatc cagtgtggtg 1380
tccttattcg aagtagccac tggcaaaaaa ccaatataca aaatatttgg gggatctcaa 1440
atcgagaact tggctttaca aaacatccag gcgcgtctaa gaatggttct ttcttatctt 1500
tttgcgcaac tgttgccgtg ggttcgtggt atcccaaact cgggtggatt gttagtactt 1560
ggtagcgcaa atgttgatga gtgcttacgt gggtatctaa caaaatatga ctgctcctcc 1620
gcagatatca accctattgg gggtatttca aaaactgact tgaaaagatt cattgcctac 1680
gcatcaaaac aatataacat gccaatcttg aatgactttt taaacgctac accaactgca 1740
gaattagaac ctatgactaa agattacgtt caatcggatg agatagatat ggggatgacg 1800
tatgaagaat tgggcgtgtt tggttaccta agaaaggttg aaaaatgtgg tccttattct 1860
atgttcttaa aacttcttca tcaatggtcc ccaaagttaa cacctcgtca aatatctgaa 1920
aaggtgaaaa gatttttctt cttctatgcc atcaacagac acaagcaaac tgttttaact 1980
cctagttatc atgctgaaca gtattcacca gaagacaaca gatttgactt acgtcctttc 2040
ttaatcaacc caagatttcc atgggcttca agaaaaattg atgaagttgt cgagcagtgt 2100
gaagcacata aaggctcaac gcttgacatt atgtctattg attag 2145
<210>10
<211>714
<212>PRT
<213>酿酒酵母
<400>10
Met Ser His Leu Ile Thr Leu Ala Thr Cys Asn Leu Asn Gln Trp Ala
1 5 10 15
Leu Asp Phe Glu Gly Asn Arg Asp Arg Ile Leu Gln Ser Ile Lys Ile
20 25 30
Ala Lys Glu Arg Gly Ala Arg Leu Arg Val Gly Pro Glu Leu Glu Ile
35 40 45
Thr Gly Tyr Gly Cys Leu Asp His Phe Leu Glu Asn Asp Val Cys Leu
50 55 60
His Ser Trp Glu Met Tyr Ala Gln Ile Ile Lys Asn Lys Glu Thr His
65 70 75 80
Gly Leu Ile Leu Asp Ile Gly Met Pro Val Leu His Lys Asn Val Arg
85 90 95
Tyr Asn Cys Arg Leu Leu Ser Leu Asp Gly Glu Ile Leu Phe Ile Arg
100 105 110
Pro Lys Ile Trp Leu Ala Asn Asp Gly Asn Tyr Arg Glu Met Arg Phe
115 120 125
Phe Thr Pro Trp Met Lys Pro Gly Val Val Glu Asp Phe Ile Leu Pro
130 135 140
Pro Glu Ile Gln Lys Val Thr Gly Gln Arg Leu Val Pro Phe Gly Asp
145 150 155 160
Ala Val Ile Asn Ser Leu Asp Thr Cys Ile Gly Thr Glu Thr Cys Glu
165 170 175
Glu Leu Phe Thr Pro Gln Ser Pro His Ile Ala Met Ser Leu Asp Gly
180 185 190
Val Glu Ile Met Thr Asn Ser Ser Gly Ser His His Glu Leu Arg Lys
195 200 205
Leu Asn Lys Arg Leu Asp Leu Ile Leu Asn Ala Thr Lys Arg Cys Gly
210 215 220
Gly Val Tyr Leu Tyr Ala Asn Gln Arg Gly Cys Asp Gly Asp Arg Leu
225 230 235 240
Tyr Tyr Asp Gly Cys Ala Leu Ile Ala Ile Asn Gly Thr Ile Val Ala
245 250 255
Gln Gly Ser Gln Phe Ser Leu Asp Asp Val Glu Val Val Thr Ala Thr
260 265 270
Val Asp Leu Glu Glu Val Arg Ser Tyr Arg Ala Ala Val Met Ser Arg
275 280 285
Gly Leu Gln Ala Ser Leu Ala Glu Ile Lys Phe Lys Arg Ile Asp Ile
290 295 300
Pro Val Glu Leu Ala Leu Met Thr Ser Arg Phe Asp Pro Thr Val Cys
305 310 315 320
Pro Thr Lys Val Arg Glu Pro Phe Tyr His Ser Pro Glu Glu Glu Ile
325 330 335
Ala Leu Gly Pro Ala Cys Trp Met Trp Asp Tyr Leu Arg Arg Cys Asn
340 345 350
Gly Thr Gly Phe Phe Leu Pro Leu Ser Gly Gly Ile Asp Ser Cys Ala
355 360 365
Thr Ala Met Ile Val His Ser Met Cys Arg Leu Val Thr Asp Ala Ala
370 375 380
Gln Asn Gly Asn Glu Gln Val Ile Lys Asp Val Arg Lys Ile Thr Arg
385 390 395 400
Ser Gly Asp Asp Trp Ile Pro Asp Ser Pro Gln Asp Leu Ala Ser Lys
405 410 415
Ile Phe His Ser Cys Phe Met Gly Thr Glu Asn Ser Ser Lys Glu Thr
420 425 430
Arg Asn Arg Ala Lys Asp Leu Ser Asn Ala Ile Gly Ser Tyr His Val
435 440 445
Asp Leu Lys Met Asp Ser Leu Val Ser Ser Val Val Ser Leu Phe Glu
450 455 460
Val Ala Thr Gly Lys Lys Pro Ile Tyr Lys Ile Phe Gly Gly Ser Gln
465 470 475 480
Ile Glu Asn Leu Ala Leu Gln Asn Ile Gln Ala Arg Leu Arg Met Val
485 490 495
Leu Ser Tyr Leu Phe Ala Gln Leu Leu Pro Trp Val Arg Gly Ile Pro
500 505 510
Asn Ser Gly Gly Leu Leu Val Leu Gly Ser Ala Asn Val Asp Glu Cys
515 520 525
Leu Arg Gly Tyr Leu Thr Lys Tyr Asp Cys Ser Ser Ala Asp Ile Asn
530 535 540
Pro Ile Gly Gly Ile Ser Lys Thr Asp Leu Lys Arg Phe Ile Ala Tyr
545 550 555 560
Ala Ser Lys Gln Tyr Asn Met Pro Ile Leu Asn Asp Phe Leu Asn Ala
565 570 575
Thr Pro Thr Ala Glu Leu Glu Pro Met Thr Lys Asp Tyr Val Gln Ser
580 585 590
Asp Glu Ile Asp Met Gly Met Thr Tyr Glu Glu Leu Gly Val Phe Gly
595 600 605
Tyr Leu Arg Lys Val Glu Lys Cys Gly Pro Tyr Ser Met Phe Leu Lys
610 615 620
Leu Leu His Gln Trp Ser Pro Lys Leu Thr Pro Arg Gln Ile Ser Glu
625 630 635 640
Lys Val Lys Arg Phe Phe Phe Phe Tyr Ala Ile Asn Arg His Lys Gln
645 650 655
Thr Val Leu Thr Pro Ser Tyr His Ala Glu Gln Tyr Ser Pro Glu Asp
660 665 670
Asn Arg Phe Asp Leu Arg Pro Phe Leu Ile Asn Pro Arg Phe Pro Trp
675 680 685
Ala Ser Arg Lys Ile Asp Glu Val Val Glu Gln Cys Glu Ala His Lys
690 695 700
Gly Ser Thr Leu Asp Ile Met Ser Ile Asp
705 710
<210>11
<211>597
<212>DNA
<213>拟南芥(Arabidopsis thaliana)
<400>11
atggcttcct catcaacgag aaagtacgag acacgaaagc gagatccaaa ctctaaaatc 60
gcagctcttc tcgttatcga catgcagaat cacttctcct ccatggccaa acccatcctc 120
aacaacgttc tcaccaccat cgacatctgc cgacgcgcct cagtccccgt attctttacg 180
cgtcacaacc acaaatcccc gaccgaccac ggcatgctcg gcgagtggtg taacggcgat 240
gtaatccttg acggaaccac cgattctgaa atcatccagg agatacaagg ccaagtaacc 300
ggaccagacg agatggtgga gaagaacacg tacagtgcgt ttaacaaaac ccgcctccag 360
gaaaacctgg aaaagatcgg agtaaaggag gtgatcgtga tcggagtgat gacgaacttg 420
tgctgtgaga caacggcgcg tgaagcgttt attaagggtt ttagggtttt tttctcgacg 480
gacgcgactg cgacgtttaa tgaggagctt cacgaggcta cgctaatgaa tctcgctttt 540
ggcttcgctt atctcgtcga ttgcgataaa ctccggcgaa gtctactcgg taactaa 597
<210>12
<211>198
<212>PRT
<213>拟南芥
<400>12
Met Ala Ser Ser Ser Thr Arg Lys Tyr Glu Thr Arg Lys Arg Asp Pro
1 5 10 15
Asn Ser Lys Ile Ala Ala Leu Leu Val Ile Asp Met Gln Asn His Phe
20 25 30
Ser Ser Met Ala Lys Pro Ile Leu Asn Asn Val Leu Thr Thr Ile Asp
35 40 45
Ile Cys Arg Arg Ala Ser Val Pro Val Phe Phe Thr Arg His Asn His
50 55 60
Lys Ser Pro Thr Asp His Gly Met Leu Gly Glu Trp Cys Asn Gly Asp
65 70 75 80
Val Ile Leu Asp Gly Thr Thr Asp Ser Glu Ile Ile Gln Glu Ile Gln
85 90 95
G1y Gln Val Thr Gly Pro Asp Glu Met Val Glu Lys Asn Thr Tyr Ser
100 105 110
Ala Phe Asn Lys Thr Arg Leu Gln Glu Asn Leu Glu Lys Ile Gly Val
115 120 125
Lys Glu Val Ile Val Ile Gly Val Met Thr Asn Leu Cys Cys Glu Thr
130 135 140
Thr Ala Arg Glu Ala Phe Ile Lys Gly Phe Arg Val Phe Phe Ser Thr
145 150 155 160
Asp Ala Thr Ala Thr Phe Asn Glu Glu Leu His Glu Ala Thr Leu Met
165 170 175
Asn Leu Ala Phe Gly Phe Ala Tyr Leu Val Asp Cys Asp Lys Leu Arg
180 185 190
Arg Ser Leu Leu Gly Asn
195
<210>13
<211>597
<212>DNA
<213>拟南芥
<400>13
atggcttctt catcatcgag aacgtacgag acacgaaagc gagagccaaa tcctaaaatc 60
gcagctcttc tcgtcatcga tatgcagaat cacttctact ctatggctga accaatcctc 120
caaaacgctc tcaccaccat cgacatctgc cgacgcgctt caatccccgt attcttcacg 180
cgccacaacc acaaatcccc aaccgaccac ggcatgctcg gagagtggtg gaacggcgat 240
ctaatcctcg acggaaccac tgattccgaa atcatcccgg aaatcaatcg ccaggtcacc 300
ggaccagacg aaatcgtgga gaagagcacg tacagtgcgt ttaacaacac gcaccttcag 360
gagaagctgg acaagatcgg agtgaaggag gtgatcgtta tcggagtgat gacgaaccta 420
tgctgtgaga cgacggcgcg tgaagcgttt gtaaaggggt ttagggtttt tttctcgacg 480
gacgcgactg cgacggttaa tgaagagctt cacgaggcta ctctaatgaa tctcgcgtat 540
ggctttgctt atctcgtcga ttgcgataga ctccggcgag gtctactcag tagttaa 597
<210>14
<211>198
<212>PRT
<213>拟南芥
<400>14
Met Ala Ser Ser Ser Ser Arg Thr Tyr Glu Thr Arg Lys Arg Glu Pro
1 5 10 15
Asn Pro Lys Ile Ala Ala Leu Leu Val Ile Asp Met Gln Asn His Phe
20 25 30
Tyr Ser Met Ala Glu Pro Ile Leu Gln Asn Ala Leu Thr Thr Ile Asp
35 40 45
Ile Cys Arg Arg Ala Ser Ile Pro Val Phe Phe Thr Arg His Asn His
50 55 60
Lys Ser Pro Thr Asp His Gly Met Leu Gly Glu Trp Trp Asn Gly Asp
65 70 75 80
Leu Ile Leu Asp Gly Thr Thr Asp Ser Glu Ile Ile Pro Glu Ile Asn
85 90 95
Arg Gln Val Thr Gly Pro Asp Glu Ile Val Glu Lys Ser Thr Tyr Ser
100 105 110
Ala Phe Asn Asn Thr His Leu Gln Glu Lys Leu Asp Lys Ile Gly Val
115 120 125
Lys Glu Val Ile Val Ile Gly Val Met Thr Asn Leu Cys Cys Glu Thr
130 135 140
Thr Ala Arg Glu Ala Phe Val Lys Gly Phe Arg Val Phe Phe Ser Thr
145 150 155 160
Asp Ala Thr Ala Thr Val Asn Glu Glu Leu His Glu Ala Thr Leu Met
165 170 175
Asn Leu Ala Tyr Gly Phe Ala Tyr Leu Val Asp Cys Asp Arg Leu Arg
180 185 190
Arg Gly Leu Leu Ser Ser
195
<210>15
<211>591
<212>DNA
<213>拟南芥
<400>15
atggccgaga gatggaggaa cacggctcta ctcgtcatcg acatgcagaa cgatttcata 60
gaggaaggtg ctgtgacgca agtgaaagga ggaaaatcta tagttcctaa tgttatcaga 120
gtcgtcgaac tcgcgaggca gcgtggtatt ctcgtaattt gggttgttcg agaacatgat 180
cgtcaaggaa gagatgttga attattcagg cgccataact acagttctga gaaagtcggg 240
ccagttatta aaggcaccgt aggagcagaa ttggttgatg gattgatgat caacgaagaa 300
gatgactata agattgtgaa aactcgtttc agtgctttct ttagtaccaa tcttcattcc 360
ttcttgcaaa cttcaggggt taccaagtta gtgattgctg gtgtgcaaac gccgaactgt 420
atccggcaaa cggtgtttga tgcagtggcg ctggattatc ccaatgtgac tgttattaca 480
gatgccacag ctgctgcaac accagagatc catactgcga atattcttga catgaagaat 540
attggagtca agactcctac attacacgag tggtccgaag aacttgcttg a 591
<210>16
<211>196
<212>PRT
<213>拟南芥
<400>16
Met Ala Glu Arg Trp Arg Asn Thr Ala Leu Leu Val Ile Asp Met Gln
1 5 10 15
Asn Asp Phe Ile Glu Glu Gly Ala Val Thr Gln Val Lys Gly Gly Lys
20 25 30
Ser Ile Val Pro Asn Val Ile Arg Val Val Glu Leu Ala Arg Gln Arg
35 40 45
Gly Ile Leu Val Ile Trp Val Val Arg Glu His Asp Arg Gln Gly Arg
50 55 60
Asp Val Glu Leu Phe Arg Arg His Asn Tyr Ser Ser Glu Lys Val Gly
65 70 75 80
Pro Val Ile Lys Gly Thr Val Gly Ala Glu Leu Val Asp Gly Leu Met
85 90 95
Ile Asn Glu Glu Asp Asp Tyr Lys Ile Val Lys Thr Arg Phe Ser Ala
100 105 110
Phe Phe Ser Thr Asn Leu His Ser Phe Leu Gln Thr Ser Gly Val Thr
115 120 125
Lys Leu Val Ile Ala Gly Val Gln Thr Pro Asn Cys Ile Arg Gln Thr
130 135 140
Val Phe Asp Ala Val Ala Leu Asp Tyr Pro Asn Val Thr Val Ile Thr
145 150 155 160
Asp Ala Thr Ala Ala Ala Thr Pro Glu Ile His Thr Ala Asn Ile Leu
165 170 175
Asp Met Lys Asn Ile Gly Val Lys Thr Pro Thr Leu His Glu Trp Ser
180 185 190
Glu Glu Leu Ala
195
<210>17
<211>1680
<212>DNA
<213>拟南芥
<400>17
atggagaaga aagaaaatgg tctcgatgga aagcaatcgg gtcgggtcat taacggaccc 60
actaacccga tggtcacacc tctgctcaac gatctttacc aattcaccat ggcttatgct 120
tattggaaag ctggcaaaca atctgagcga tctgtgtttg atctgtattt tcgtaagaat 180
ccttttggtg gagaatacac tatctttgct ggtttagaag aatgcatcaa atttctcgct 240
aatttcaatt tgactgatga agagatcgat ttcgttcgtg attcgttacc tggatgtgag 300
gaagctttct gtgattatct tcgagggctt gattgttctg acattgaagt gtatgccatt 360
tcggaaggat cagttgtttt tcctaaagtt cctttactca gaatcgaagg tcctgttgct 420
gtggtgcaat tgttggaaac tccattcctc aatctcatca attacgcatc tttggttgct 480
acaaatgcag caagacatcg gtttgttgca ggaaaatcta agcttctgct tgagtttggt 540
gctagaagag ctcagggacc cgatggtgca ataagcgcat caaagtattg ctaccttgga 600
ggttttgatg caacaagtaa tgttgcagcg ggaaaactgt ttgggatacc cctccgtggt 660
actcattccc atgcttttgt tagctcattc atgagccttg atgaaattgt tgacaaagtg 720
cttcgaagtt ctgatgggaa aagcacttgt aaggatttta tatgtttggt ccaaacttgc 780
ctaacaaaga ttcagaattc atcttcatta caaggaattt tttccgagac aaatcaaagc 840
gagcttgcag cgttcatttc atatgcactg gcattcccaa actcctttct cgctcttgta 900
gacacttatg atgtgatgaa gagtggtatt ccaaacttct gtgctgttgc tctagcactt 960
aatgaattgg gatacaaagc agtaggcatt agactggatt caggtgactt agcctatctt 1020
tctactgagg tcaggaaatt cttttgtgcc atagagagag acctcaaagt tcctgatttc 1080
gggaagatga tcgtcactgc tagtaacgat ctaaacgaag agacagtcga tgctctaaat 1140
aaacagggtc atgaagtaga tgcatttgga attggaacca acttagtgac ttgctatgcg 1200
caagctgcgt taggttgtgt tttcaaactt gtggaaataa acaatcagcc tcggatcaaa 1260
ctttctgaag atgttactaa ggtatcgatt ccatgtaaaa agcgtactta cagattgttc 1320
ggaaaagagg gttaccctct tgttgatata atgactggag agaacgaacc acctccaaag 1380
gtcggtgaaa ggttactttg ccgtcatcca ttcaatgaat caaaaagggc ttatgtggtt 1440
ccacaacgcg ttgaagagct tctgaaatgt tattggcgtg gcaatgcaga tgaagctagg 1500
gaagagctag agccattgaa agagctaaga aatcgttgca tcaaacagct cgaaaatatg 1560
cgacccgatc atatgagaag attaaaccct actccttata aggttagtgt cagcgccaag 1620
ttgtatgact tcatccactt cctctggctc aacgaagctc ctgtcggtga actgcattga 1680
<210>18
<211>559
<212>PRT
<213>拟南芥
<400>18
Met Glu Lys Lys Glu Asn Gly Leu Asp Gly Lys Gln Ser Gly Arg Val
1 5 10 15
Ile Asn Gly Pro Thr Asn Pro Met Val Thr Pro Leu Leu Asn Asp Leu
20 25 30
Tyr Gln Phe Thr Met Ala Tyr Ala Tyr Trp Lys Ala Gly Lys Gln Ser
35 40 45
Glu Arg Ser Val Phe Asp Leu Tyr Phe Arg Lys Asn Pro Phe Gly Gly
50 55 60
Glu Tyr Thr Ile Phe Ala Gly Leu Glu Glu Cys Ile Lys Phe Leu Ala
65 70 75 80
Asn Phe Asn Leu Thr Asp Glu Glu Ile Asp Phe Val Arg Asp Ser Leu
85 90 95
Pro Gly Cys Glu Glu Ala Phe Cys Asp Tyr Leu Arg Gly Leu Asp Cys
100 105 110
Ser Asp Ile Glu Val Tyr Ala Ile Ser Glu Gly Ser Val Val Phe Pro
115 120 125
Lys Val Pro Leu Leu Arg Ile Glu Gly Pro Val Ala Val Val Gln Leu
130 135 140
Leu Glu Thr Pro Phe Leu Asn Leu Ile Asn Tyr Ala Ser Leu Val Ala
145 150 155 160
Thr Asn Ala Ala Arg His Arg Phe Val Ala Gly Lys Ser Lys Leu Leu
165 170 175
Leu Glu Phe Gly Ala Arg Arg Ala Gln Gly Pro Asp Gly Ala Ile Ser
180 185 190
Ala Ser Lys Tyr Cys Tyr Leu Gly Gly Phe Asp Ala Thr Ser Asn Val
195 200 205
Ala Ala Gly Lys Leu Phe Gly Ile Pro Leu Arg Gly Thr His Ser His
210 215 220
Ala Phe Val Ser Ser Phe Met Ser Leu Asp Glu Ile Val Asp Lys Val
225 230 235 240
Leu Arg Ser Ser Asp Gly Lys Ser Thr Cys Lys Asp Phe Ile Cys Leu
245 250 255
Val Gln Thr Cys Leu Thr Lys Ile Gln Asn Ser Ser Ser Leu Gln Gly
260 265 270
Ile Phe Ser Glu Thr Asn Gln Ser Glu Leu Ala Ala Phe Ile Ser Tyr
275 280 285
Ala Leu Ala Phe Pro Asn Ser Phe Leu Ala Leu Val Asp Thr Tyr Asp
290 295 300
Val Met Lys Ser Gly Ile Pro Asn Phe Cys Ala Val Ala Leu Ala Leu
305 310 315 320
Asn Glu Leu Gly Tyr Lys Ala Val Gly Ile Arg Leu Asp Ser Gly Asp
325 330 335
Leu Ala Tyr Leu Ser Thr Glu Val Arg Lys Phe Phe Cys Ala Ile Glu
340 345 350
Arg Asp Leu Lys Val Pro Asp Phe Gly Lys Met Ile Val Thr Ala Ser
355 360 365
Asn Asp Leu Asn Glu Glu Thr Val Asp Ala Leu Asn Lys Gln Gly His
370 375 380
Glu Val Asp Ala Phe Gly Ile Gly Thr Asn Leu Val Thr Cys Tyr Ala
385 390 395 400
Gln Ala Ala Leu Gly Cys Val Phe Lys Leu Val Glu Ile Asn Asn Gln
405 410 415
Pro Arg Ile Lys Leu Ser Glu Asp Val Thr Lys Val Ser Ile Pro Cys
420 425 430
Lys Lys Arg Thr Tyr Arg Leu Phe Gly Lys Glu Gly Tyr Pro Leu Val
435 440 445
Asp Ile Met Thr Gly Glu Asn Glu Pro Pro Pro Lys Val Gly Glu Arg
450 455 460
Leu Leu Cys Arg His Pro Phe Asn Glu Ser Lys Arg Ala Tyr Val Val
465 470 475 480
Pro Gln Arg Val Glu Glu Leu Leu Lys Cys Tyr Trp Arg Gly Asn Ala
485 490 495
Asp Glu Ala Arg Glu Glu Leu Glu Pro Leu Lys Glu Leu Arg Asn Arg
500 505 510
Cys Ile Lys Gln Leu Glu Asn Met Arg Pro Asp His Met Arg Arg Leu
515 520 525
Asn Pro Thr Pro Tyr Lys Val Ser Val Ser Ala Lys Leu Tyr Asp Phe
530 535 540
Ile His Phe Leu Trp Leu Asn Glu Ala Pro Val Gly Glu Leu His
545 550 555
<210>19
<211>1674
<212>DNA
<213>拟南芥
<400>19
atggagccga aagagaacgg ctcagaattg ggtcagaaga tcattgacgg accaacgaat 60
ccaatggtca cacctttact caatgatctt tatcaattca ccatggctta tgcttattgg 120
aaagctggca aacacaacga acgatccgtt ttcgatctgt attttcgtaa gaacccattt 180
ggtggtgagt acactgtgtt tgctggatta gaagagtgtg ttaagttctt agccaatttc 240
aaattgactg atgaagaaat cgatttcgtt caagagtgtt tgcctggatc tgaggaagct 300
ttttgtgatt atcttagagg gcttgattgt tctgatgttg aagtttatgc aattccggaa 360
ggatcagttg tttttcctaa agtacctctc atgagagttg aaggacctgt tggtgttgtt 420
caattgttgg aaactccatt cctcaatctt gtcaattttg catctttggt agctactaac 480
gcagctaggc atcgctttgt tgccggaaaa tctaagagtc tactcgagtt tggtgctcga 540
agggctcagg gtccggatgg tgcaataagc gcatcaaaat attgctacct tggaggtttt 600
gatgcaacaa gtaatgtagc agctggaaaa ctttttggga ttcctcttcg tggaacacac 660
tctcatgctt atgttagctc attcatgagt actgatgaga ttgttgacaa agtacttcgt 720
agtgctgatg ggaaaaccac gtgcgaggat tttgttagtc atgttcagac atggttaaaa 780
aagattcagt attcaccatc tctaagtggc attttctctg agacaaatca aagcgagcta 840
gcagctttca cctcatatgc actggcattc cccaaaactt ttcttgccct cgtagataca 900
tacgatgtga tgaagagtgg aatccctaac ttctgtgcag ttgctttagc actcaatgac 960
tttggatata aagcattagg tattagactg gattcaggtg atttagctta tctatctaga 1020
gaggccagaa atttcttctg cacggtagag agagaactaa aagtgcctgg ttttgggaag 1080
atggtcgtca ctgctagtaa tgatctaaat gaagagacga ttgacgcttt aaataaacag 1140
ggacatgagg tggatgcttt tggcatcggg acctacttgg tcacttgcta ttcacaagcg 1200
gccttaggtt gcgttttcaa acttgtggag ataaacaatc agcctcggat taaactttct 1260
gaagatgtta caaaggtatc aataccgtgt aaaaagcgaa gttacagatt atacggcaaa 1320
gaaggttacc ctctggtaga tataatgact ggagagaacg aaccacctcc aaaggttggt 1380
gagcgtttac tttgtcgtca cccattcaac gaatccaaaa gagcatatgt agtgccacaa 1440
cgtgtcgaag agctcctcaa atgttattgg cgtggaagtg cagatgaagc aagagaagta 1500
ttaccgcctt tgaaagagat aagagaccgt tgcatcaaac agctcgaaaa catgcgacct 1560
gatcatatga ggagattaaa cccaactcct tataaggtta gtgtaagcgc aaagctgtac 1620
gatttcatcc acttcttatg gctaaacgaa gcacctgttg gtgaattgca gtga 1674
<210>20
<211>557
<212>PRT
<213>拟南芥
<400>20
Met Glu Pro Lys Glu Asn Gly Ser Glu Leu Gly Gln Lys Ile Ile Asp
1 5 10 15
Gly Pro Thr Asn Pro Met Val Thr Pro Leu Leu Asn Asp Leu Tyr Gln
20 25 30
Phe Thr Met Ala Tyr Ala Tyr Trp Lys Ala Gly Lys His Asn Glu Arg
35 40 45
Ser Val Phe Asp Leu Tyr Phe Arg Lys Asn Pro Phe Gly Gly Glu Tyr
50 55 60
Thr Val Phe Ala Gly Leu Glu Glu Cys Val Lys Phe Leu Ala Asn Phe
65 70 75 80
Lys Leu Thr Asp Glu Glu Ile Asp Phe Val Gln Glu Cys Leu Pro Gly
85 90 95
Ser Glu Glu Ala Phe Cys Asp Tyr Leu Arg Gly Leu Asp Cys Ser Asp
100 105 110
Val Glu Val Tyr Ala Ile Pro Glu Gly Ser Val Val Phe Pro Lys Val
115 120 125
Pro Leu Met Arg Val Glu Gly Pro Val Gly Val Val Gln Leu Leu Glu
130 135 140
Thr Pro Phe Leu Asn Leu Val Asn Phe Ala Ser Leu Val Ala Thr Asn
145 150 155 160
Ala Ala Arg His Arg Phe Val Ala Gly Lys Ser Lys Ser Leu Leu Glu
165 170 175
Phe Gly Ala Arg Arg Ala Gln Gly Pro Asp Gly Ala Ile Ser Ala Ser
180 185 190
Lys Tyr Cys Tyr Leu Gly Gly Phe Asp Ala Thr Ser Asn Val Ala Ala
195 200 205
Gly Lys Leu Phe Gly Ile Pro Leu Arg Gly Thr His Ser His Ala Tyr
210 215 220
Val Ser Ser Phe Met Ser Thr Asp Glu Ile Val Asp Lys Val Leu Arg
225 230 235 240
Ser Ala Asp Gly Lys Thr Thr Cys Glu Asp Phe Val Ser His Val Gln
245 250 255
Thr Trp Leu Lys Lys Ile Gln Tyr Ser Pro Ser Leu Ser Gly Ile Phe
260 265 270
Ser Glu Thr Asn Gln Ser Glu Leu Ala Ala Phe Thr Ser Tyr Ala Leu
275 280 285
Ala Phe Pro Lys Thr Phe Leu Ala Leu Val Asp Thr Tyr Asp Val Met
290 295 300
Lys Ser Gly Ile Pro Asn Phe Cys Ala Val Ala Leu Ala Leu Asn Asp
305 310 315 320
Phe Gly Tyr Lys Ala Leu Gly Ile Arg Leu Asp Ser Gly Asp Leu Ala
325 330 335
Tyr Leu Ser Arg Glu Ala Arg Asn Phe Phe Cys Thr Val Glu Arg Glu
340 345 350
Leu Lys Val Pro Gly Phe Gly Lys Met Val Val Thr Ala Ser Asn Asp
355 360 365
Leu Asn Glu Glu Thr Ile Asp Ala Leu Asn Lys Gln Gly His Glu Val
370 375 380
Asp Ala Phe Gly Ile Gly Thr Tyr Leu Val Thr Cys Tyr Ser Gln Ala
385 390 395 400
Ala Leu Gly Cys Val Phe Lys Leu Val Glu Ile Asn Asn Gln Pro Arg
405 410 415
Ile Lys Leu Ser Glu Asp Val Thr Lys Val Ser Ile Pro Cys Lys Lys
420 425 430
Arg Ser Tyr Arg Leu Tyr Gly Lys Glu Gly Tyr Pro Leu Val Asp Ile
435 440 445
Met Thr Gly Glu Asn Glu Pro Pro Pro Lys Val Gly Glu Arg Leu Leu
450 455 460
Cys Arg His Pro Phe Asn Glu Ser Lys Arg Ala Tyr Val Val Pro Gln
465 470 475 480
Arg Val Glu Glu Leu Leu Lys Cys Tyr Trp Arg Gly Ser Ala Asp Glu
485 490 495
Ala Arg Glu Val Leu Pro Pro Leu Lys Glu Ile Arg Asp Arg Cys Ile
500 505 510
Lys Gln Leu Glu Asn Met Arg Pro Asp His Met Arg Arg Leu Asn Pro
515 520 525
Thr Pro Tyr Lys Val Ser Val Ser Ala Lys Leu Tyr Asp Phe Ile His
530 535 540
Phe Leu Trp Leu Asn Glu Ala Pro Val Gly Glu Leu Gln
545 550 555
<210>21
<211>717
<212>DNA
<213>拟南芥
<400>21
atggatgtcc cgttaccagt cgagaaatta tcttatggat caaacactga ggacaaaact 60
tgtgtagtgc ttgtggcaac tgggagtttc aatcctccta ctttcatgca tttacgcatg 120
tttgagctgg cgagagatga attacgctca aaaggatttc atgttcttgg aggatatatg 180
tctcctgtta atgatgcata taagaagaag ggccttttat ctgcagaaca tcgtttagag 240
atgtgtaatg tatcatgtca aagctctgac tttgtaatgg ttgatccgtg ggaggcatct 300
caaagcaact accaacgaac tttgacggtt ttatcaaggg tcaagacttt cttaacaaca 360
aatcgacatg tacccgagga atctctcaaa gtcatgctac tatgtggctc ggatttactg 420
ctatctttct gcactcccgg tgtttggatc cctgaacagt taagaactat ttgcaaagat 480
tatggcattg tgtgcatccg tagagaagga caagatgttg aaaatatgat ctctggtgac 540
gaaatcttaa acgaaaactg tgctaacgtc aaaatcgttg acaatactgt tcctaatcaa 600
atcagttcga gtagattaag gcaatgcatt tcgcgagggt tatcggttaa atacttgact 660
gaagatggag taatagatta tatcagacaa catcaactat acactgagct cacatga 717
<210>22
<211>238
<212>PRT
<213>拟南芥
<400>22
Met Asp Val Pro Leu Pro Val Glu Lys Leu Ser Tyr Gly Ser Asn Thr
1 5 10 15
Glu Asp Lys Thr Cys Val Val Leu Val Ala Thr Gly Ser Phe Asn Pro
20 25 30
Pro Thr Phe Met His Leu Arg Met Phe Glu Leu Ala Arg Asp Glu Leu
35 40 45
Arg Ser Lys Gly Phe His Val Leu Gly Gly Tyr Met Ser Pro Val Asn
50 55 60
Asp Ala Tyr Lys Lys Lys Gly Leu Leu Ser Ala Glu His Arg Leu Glu
65 70 75 80
Met Cys Asn Val Ser Cys Gln Ser Ser Asp Phe Val Met Val Asp Pro
85 90 95
Trp Glu Ala Ser Gln Ser Asn Tyr Gln Arg Thr Leu Thr Val Leu Ser
100 105 110
Arg Val Lys Thr Phe Leu Thr Thr Asn Arg His Val Pro Glu Glu Ser
115 120 125
Leu Lys Val Met Leu Leu Cys Gly Ser Asp Leu Leu Leu Ser Phe Cys
130 135 140
Thr Pro Gly Val Trp Ile Pro Glu Gln Leu Arg Thr Ile Cys Lys Asp
145 150 155 160
Tyr Gly Ile Val Cys Ile Arg Arg Glu Gly Gln Asp Val Glu Asn Met
165 170 175
Ile Ser Gly Asp Glu Ile Leu Asn Glu Asn Cys Ala Asn Val Lys Ile
180 185 190
Val Asp Asn Thr Val Pro Asn Gln Ile Ser Ser Ser Arg Leu Arg Gln
195 200 205
Cys Ile Ser Arg Gly Leu Ser Val Lys Tyr Leu Thr Glu Asp Gly Val
210 215 220
Ile Asp Tyr Ile Arg Gln His Gln Leu Tyr Thr Glu Leu Thr
225 230 235
<210>23
<211>2178
<212>DNA
<213>拟南芥
<400>23
atgaggctgt tgaaggttgc tacgtgtaac ttgaaccaat gggccatgga tttcgagagc 60
aacatgaaga acatcaaggc ttcgatcgct gaggcaaagg ctgctggtgc tgttatcagg 120
cttggacccg agctcgaggt cactggctat ggttgcgagg atcacttctt ggaactcgac 180
actgtcactc atgcgtggga gtgtttgaag gaattgctgc ttggtgattg gacggatgat 240
attttgtgca gcataggaat gcctgtgatt aaaggagcag agcgttataa ctgccaggtt 300
ctctgtatga acagaagaat catcatgatt cgaccgaaaa tgtggctcgc aaacgatgga 360
aactataggg agctacggtg gttcacagct tggaagcaga gagaagagct agaggaattt 420
cagctcccca ttgaaatttc agaggctttg gagcagaaat cagtcccttt tggttatggt 480
tacatccagt ttatcgacac ggctgttgca gctgaagtct gtgaggaact gtttagtcca 540
cttcctcctc atgccgagct cgcattgaat ggtgttgaag tatttatgaa tgcaagtggg 600
agtcatcacc aacttaggaa actagatatt cgtctgaatg cttttatggg ggctactcat 660
gctcgtggtg gggtgtatat gtacagtaat caacaaggat gcgatggtag ccgcttatac 720
tacgatggat gtgcatgtat tgttgtaaac gggaatgttg ttgctcaagg ctcacaattc 780
tcgttgagag acgttgaggt catcatttca caagtggatc ttgatgcggt tgctagcctt 840
cgtggatcta taagtagctt tcaggaacaa gcaagctgca aggttaaagt atcttcagta 900
gctgtgccct gtagacttac acagtccttc aacctgaaaa tgacactaag cagtccgaag 960
aagatcattt accactctcc acaagaagaa atagcctttg gtcccgcttg ctggatgtgg 1020
gactatttga gaagaagtgg cgcttcagga tttttgcttc ctctttctgg cggagcagac 1080
agctcctccg tggcagctat tgttggctgc atgtgccaac ttgttgttaa agagattgca 1140
aagggagatg agcaagtaaa agctgatgcg aaccgaattg ggaattatgc taatgggcag 1200
tttcctactg atagcaaaga gtttgccaaa cgaatatttt acactgtctt tatgggttct 1260
gaaaacagtt ctgaggagac aaaaaggcgt tcaaagcagc tggcagacga gattggtgct 1320
tggcatcttg atgtttgcat agatggtgtt gtctctgcag ttttatcatt atttcaaaca 1380
gttacaggca agcgaccaag gtataaggtt gatggaggat caaatgctga gaaccttggg 1440
ttgcagaaca ttcaagcccg gatgagaatg gtgttagcat ttatgttagc gtctctcttg 1500
ccttgggttc atagcaaacc aggcttttac cttgttctag gcagctccaa cgttgatgaa 1560
ggacttcgtg gttacctgac aaagtatgat tgcagctcag cagacataaa tcctatagga 1620
agtatcagta aaatggattt gaggttgttc ttaaaatggg ctgcaacgaa tctcggatat 1680
ccatccttgg cagagataga agctgctcca ccaacagctg agcttgagcc cattcgttct 1740
gactattctc agctcgatga agtcgacatg ggaatgacat atgaagagct ttcagtctat 1800
ggaaggatga ggaagatatt ccgttgtgga ccagtatcta tgttcaagaa tctatgttac 1860
aagtggggaa caaagctaag cccagcagaa gtagctgaga aagtgaagta tttcttcaaa 1920
tattattcga tcaatcgaca caaaatgact gtcctcacac cgtcttatca cgctgagagt 1980
tactccccag aggacaacag attcgatctg aggcagtttc tgtacaacag caagtggcca 2040
taccagttta agaagattga cgagattgtt gacagcttaa atggtgactc agttgctttc 2100
ccggaagaag aagcaaactc caacaaagaa attggagttg tagcagcaaa ctccggagac 2160
ccaagtgcgg gtctctga 2178
<210>24
<211>725
<212>PRT
<213>拟南芥
<400>24
Met Arg Leu Leu Lys Val Ala Thr Cys Asn Leu Asn Gln Trp Ala Met
1 5 10 15
Asp Phe Glu Ser Asn Met Lys Asn Ile Lys Ala Ser Ile Ala Glu Ala
20 25 30
Lys Ala Ala Gly Ala Val Ile Arg Leu Gly Pro Glu Leu Glu Val Thr
35 40 45
Gly Tyr Gly Cys Glu Asp His Phe Leu Glu Leu Asp Thr Val Thr His
50 55 60
Ala Trp Glu Cys Leu Lys Glu Leu Leu Leu Gly Asp Trp Thr Asp Asp
65 70 75 80
Ile Leu Cys Ser Ile Gly Met Pro Val Ile Lys Gly Ala Glu Arg Tyr
85 90 95
Asn Cys Gln Val Leu Cys Met Asn Arg Arg Ile Ile Met Ile Arg Pro
100 105 110
Lys Met Trp Leu Ala Asn Asp Gly Asn Tyr Arg Glu Leu Arg Trp Phe
115 120 125
Thr Ala Trp Lys Gln Arg Glu Glu Leu Glu Glu Phe Gln Leu Pro Ile
130 135 140
Glu Ile Ser Glu Ala Leu Glu Gln Lys Ser Val Pro Phe Gly Tyr Gly
145 150 155 160
Tyr Ile Gln Phe Ile Asp Thr Ala Val Ala Ala Glu Val Cys Glu Glu
165 170 175
Leu Phe Ser Pro Leu Pro Pro His Ala Glu Leu Ala Leu Asn Gly Val
180 185 190
Glu Val Phe Met Asn Ala Ser Gly Ser His His Gln Leu Arg Lys Leu
195 200 205
Asp Ile Arg Leu Asn Ala Phe Met Gly Ala Thr His Ala Arg Gly Gly
210 215 220
Val Tyr Met Tyr Ser Asn Gln Gln Gly Cys Asp Gly Ser Arg Leu Tyr
225 230 235 240
Tyr Asp Gly Cys Ala Cys Ile Val Val Asn Gly Asn Val Val Ala Gln
245 250 255
Gly Ser Gln Phe Ser Leu Arg Asp Val Glu Val Ile Ile Ser Gln Val
260 265 270
Asp Leu Asp Ala Val Ala Ser Leu Arg Gly Ser Ile Ser Ser Phe Gln
275 280 285
Glu Gln Ala Ser Cys Lys Val Lys Val Ser Ser Val Ala Val Pro Cys
290 295 300
Arg Leu Thr Gln Ser Phe Asn Leu Lys Met Thr Leu Ser Ser Pro Lys
305 310 315 320
Lys Ile Ile Tyr His Ser Pro Gln Glu Glu Ile Ala Phe Gly Pro Ala
325 330 335
Cys Trp Met Trp Asp Tyr Leu Arg Arg Ser Gly Ala Ser Gly Phe Leu
340 345 350
Leu Pro Leu Ser Gly Gly Ala Asp Ser Ser Ser Val Ala Ala Ile Val
355 360 365
Gly Cys Met Cys Gln Leu Val Val Lys Glu Ile Ala Lys Gly Asp Glu
370 375 380
Gln Val Lys Ala Asp Ala Asn Arg Ile Gly Asn Tyr Ala Asn Gly Gln
385 390 395 400
Phe Pro Thr Asp Ser Lys Glu Phe Ala Lys Arg Ile Phe Tyr Thr Val
405 410 415
Phe Met Gly Ser Glu Asn Ser Ser Glu Glu Thr Lys Arg Arg Ser Lys
420 425 430
Gln Leu Ala Asp Glu Ile Gly Ala Trp His Leu Asp Val Cys Ile Asp
435 440 445
Gly Val Val Ser Ala Val Leu Ser Leu Phe Gln Thr Val Thr Gly Lys
450 455 460
Arg Pro Arg Tyr Lys Val Asp Gly Gly Ser Asn Ala Glu Asn Leu Gly
465 470 475 480
Leu Gln Asn Ile Gln Ala Arg Met Arg Met Val Leu Ala Phe Met Leu
485 490 495
Ala Ser Leu Leu Pro Trp Val His Ser Lys Pro Gly Phe Tyr Leu Val
500 505 510
Leu Gly Ser Ser Asn Val Asp Glu Gly Leu Arg Gly Tyr Leu Thr Lys
515 520 525
Tyr Asp Cys Ser Ser Ala Asp Ile Asn Pro Ile Gly Ser Ile Ser Lys
530 535 540
Met Asp Leu Arg Leu Phe Leu Lys Trp Ala Ala Thr Asn Leu Gly Tyr
545 550 555 560
Pro Ser Leu Ala Glu Ile Glu Ala Ala Pro Pro Thr Ala Glu Leu Glu
565 570 575
Pro Ile Arg Ser Asp Tyr Ser Gln Leu Asp Glu Val Asp Met Gly Met
580 585 590
Thr Tyr Glu Glu Leu Ser Val Tyr Gly Arg Met Arg Lys Ile Phe Arg
595 600 605
Cys Gly Pro Val Ser Met Phe Lys Asn Leu Cys Tyr Lys Trp Gly Thr
610 615 620
Lys Leu Ser Pro Ala Glu Val Ala Glu Lys Val Lys Tyr Phe Phe Lys
625 630 635 640
Tyr Tyr Ser Ile Asn Arg His Lys Met Thr Val Leu Thr Pro Ser Tyr
645 650 655
His Ala Glu Ser Tyr Ser Pro Glu Asp Asn Arg Phe Asp Leu Arg Gln
660 665 670
Phe Leu Tyr Asn Ser Lys Trp Pro Tyr Gln Phe Lys Lys Ile Asp Glu
675 680 685
Ile Val Asp Ser Leu Asn Gly Asp Ser Val Ala Phe Pro Glu Glu Glu
690 695 700
Ala Asn Ser Asn Lys Glu Ile Gly Val Val Ala Ala Asn Ser Gly Asp
705 710 715 720
Pro Ser Ala Gly Leu
725
<210>25
<211>11811
<212>DNA
<213>人工
<220>
<223>pTVE467
<400>25
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttattta 540
tccacgacat tgatgttgtg ggccttcaac tcttccttaa ccttattgat gacttcggga 600
tcatcgctga tgggtcttgt gtaatccagc aggacagtgg tcttataacc tagttctgca 660
gcggaaatgg cggtggcttt gacacaatac tccaaagcta caccgacaat gtaaacctcg 720
tctgtatgat gcttttctaa gtacttgttc atgtcggtct tatggaagtt ccagatgtcg 780
tggaaggcgg agtagtattc acggtcagtc aagaaaccct tgtcgacaat cttaatatgc 840
ttagtgacca cttggtccat tatttggtca accaattgac taccccaggt gtttttcaca 900
cagtgtacgg gccacaaaat accctcttgc gtggaatcat cgcctggcct tggagagtgg 960
taggtgtatg ttgaataggg ttctttatct ttatggttct ttgcgaacga aatatgtctg 1020
gaagggtgcc aatctctggt gaccacaatc ctgtgccagt ctctatcagc atcttgcatc 1080
aaatccgaga taggattgat taattcctca ccttttggaa cagtcaagga acctaaaggt 1140
gaaataaaat cattttgcat atcaacaaca attaaagtct ccatggtttt ggtttaataa 1200
gaagagaaaa gagttctttt gttatggctg aagtaataga gaaatgagct cgagtcctct 1260
ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg 1320
cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg 1380
gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt 1440
cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc 1500
caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga 1560
ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga 1620
ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttgacgaaga 1680
ttttcttctt gtcattgagt cgtaaaagac tctgtatgaa ctgttcgcca gtcttcacgg 1740
cgagttctgt tagatcctcg atctgaattt ttgactccat gtatggtgca tatggcgcgc 1800
catatgcccg ggccctgtac agcggccgcg ttaacgcgta tactctagag cgatcgcccg 1860
ggccggccat ttaaatgaat tcgagctcgg tacccaaacg cggccgcaag ctataacttc 1920
gtatagcata cattatacga agttattcga ctctagagga tcccaattcc catgcatgga 1980
gtcaaagatt caaatagagg acacttctcg aactcggccg tcgaactcgg ccgtcgagta 2040
catggtcgat aagaaaaggc aatttgtaga tgttaattcc catcttgaaa gaaatatagt 2100
ttaaatattt attgataaaa taacaagtca ggtattatag tccaagcaaa aacataaatt 2160
tattgatgca agtttaaatt cagaaatatt tcaataactg attatatcag ctggtacatt 2220
gccgtagatg aaagactgag tgcgatatta tgtgtaatac ataaattgat gatatagcta 2280
gcttagctca tcgggggatc ctagacgcgt gagatcagat ctcggtgacg ggcaggaccg 2340
gacggggcgg taccggcagg ctgaagtcca gctgccagaa acccacgtca tgccagttcc 2400
cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc gcctcgtgca 2460
tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg aagccctgtg 2520
cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc 2580
ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt gccttccagg 2640
ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc cagggatagc 2700
gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga 2760
agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg 2820
cctcggtggc acggcggatg tcggccgggc gtcgttctgg gtccattgtt cttctttact 2880
ctttgtgtga ctgaggtttg gtctagtgct ttggtcatct atatataatg ataacaacaa 2940
tgagaacaag ctttggagtg atcggagggt ctaggataca tgagattcaa gtggactagg 3000
atctacaccg ttggattttg agtgtggata tgtgtgaggt taattttact tggtaacggc 3060
cacaaaggcc taaggagagg tgttgagacc cttatcggct tgaaccgctg gaataatgcc 3120
acgtggaaga taattccatg aatcttatcg ttatctatga gtgaaattgt gtgatggtgg 3180
agtggtgctt gctcatttta cttgcctggt ggacttggcc ctttccttat ggggaattta 3240
tattttactt actatagagc tttcatacct tttttttacc ttggatttag ttaatatata 3300
atggtatgat tcatgaataa aaatgggaaa tttttgaatt tgtactgcta aatgcataag 3360
attaggtgaa actgtggaat atatattttt ttcatttaaa agcaaaattt gccttttact 3420
agaattataa atatagaaaa atatataaca ttcaaataaa aatgaaaata agaactttca 3480
aaaaacagaa ctatgtttaa tgtgtaaaga ttagtcgcac atcaagtcat ctgttacaat 3540
atgttacaac aagtcataag cccaacaaag ttagcacgtc taaataaact aaagagtcca 3600
cgaaaatatt acaaatcata agcccaacaa agttattgat caaaaaaaaa aaacgcccaa 3660
caaagctaaa caaagtccaa aaaaaacttc tcaagtctcc atcttccttt atgaacattg 3720
aaaactatac acaaaacaag tcagataaat ctctttctgg gcctgtcttc ccaacctcct 3780
acatcacttc cctatcggat tgaatgtttt acttgtacct tttccgttgc aatgatattg 3840
atagtatgtt tgtgaaaact aatagggtta acaatcgaag tcatggaata tggatttggt 3900
ccaagatttt ccgagagctt tctagtagaa agcccatcac cagaaattta ctagtaaaat 3960
aaatcaccaa ttaggtttct tattatgtgc caaattcaat ataattatag aggatatttc 4020
aaatgaaaac gtatgaatgt tattagtaaa tggtcaggta agacattaaa aaaatcctac 4080
gtcagatatt caactttaaa aattcgatca gtgtggaatt gtacaaaaat ttgggatcta 4140
ctatatatat ataatgcttt acaacacttg gatttttttt tggaggctgg aatttttaat 4200
ctacatattt gttttggcca tgcaccaact cattgtttag tgtaatactt tgattttgtc 4260
aaatatatgt gttcgtgtat atttgtataa gaatttcttt gaccatatac acacacacat 4320
atatatatat atatatatat tatatatcat gcacttttaa ttgaaaaaat aatatatata 4380
tatatagtgc attttttcta acaaccatat atgttgcgat tgatctgcaa aaatactgct 4440
agagtaatga aaaatataat ctattgctga aattatctca gatgttaaga ttttcttaaa 4500
gtaaattctt tcaaatttta gctaaaagtc ttgtaataac taaagaataa tacacaatct 4560
cgaccacgga aaaaaaacac ataataaatt tgaatttcga ccgcggtacc cggaattggg 4620
ttataattac ctcaggtcga ggaattaatt cggtacgtac ctaataactt cgtatagcat 4680
acattatacg aagttatatg gatctcgagg cattacggca ttacggcact cgcgagggtc 4740
ccaattcgag catggagcca tttacaattg aatatatcct gccgccgctg ccgctttgca 4800
cccggtggag cttgcatgtt ggtttctacg cagaactgag ccggttaggc agataatttc 4860
cattgagaac tgagccatgt gcaccttccc cccaacacgg tgagcgacgg ggcaacggag 4920
tgatccacat gggactttta aacatcatcc gtcggatggc gttgcgagag aagcagtcga 4980
tccgtgagat cagccgacgc accgggcagg cgcgcaacac gatcgcaaag tatttgaacg 5040
caggtacaat cgagccgacg ttcacggtac cggaacgacc aagcaagcta gcttagtaaa 5100
gccctcgcta gattttaatg cggatgttgc gattacttcg ccaactattg cgataacaag 5160
aaaaagccag cctttcatga tatatctccc aatttgtgta gggcttatta tgcacgctta 5220
aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg tgcttagtgc 5280
atctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga attgttagac 5340
attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact 5400
gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa 5460
gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct 5520
tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat 5580
ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg 5640
cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca 5700
aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg 5760
ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc 5820
gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag 5880
cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag 5940
ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt 6000
gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt 6060
cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca 6120
ccgcttccct catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt 6180
tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc 6240
gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc 6300
cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact 6360
tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt 6420
tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct 6480
ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc 6540
tgttcttcta cggcaagtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc 6600
tcggccgtcc gggcgcttgc cggtggtgct gaccccggat gaagtctcta gagctctaga 6660
gggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag cttctgtatg 6720
gaacgggcat gcggatcagt gagggtttgc aactgcgggt caaggatctg gatttcgatc 6780
acggcacgat catcgtgcgg gagggcaagg gctccaagga tcgggccttg atgttacccg 6840
agagcttggc acccagcctg cgcgagcagg gatcgatcca acccctccgc tgctatagtg 6900
cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct 6960
aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta 7020
gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg 7080
ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc 7140
aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca 7200
ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg 7260
tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg 7320
agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca 7380
ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt 7440
ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt 7500
ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc 7560
aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt 7620
accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct 7680
tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag 7740
aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga 7800
gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac 7860
cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc 7920
cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta 7980
aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg 8040
caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg 8100
accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc 8160
gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta 8220
accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc 8280
gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag 8340
gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc 8400
gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc 8460
tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg 8520
gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc 8580
actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag 8640
gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga 8700
aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg 8760
ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc 8820
cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg 8880
agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt 8940
agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac 9000
gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt 9060
gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg 9120
caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt 9180
gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc 9240
gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc 9300
gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct 9360
ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc 9420
gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga 9480
ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc 9540
ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg 9600
ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg 9660
aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat 9720
gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt 9780
gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga 9840
gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt 9900
tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg 9960
ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg 10020
cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga 10080
cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg 10140
ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct 10200
agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac 10260
gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa 10320
gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga 10380
tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc 10440
ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct 10500
gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat 10560
ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga 10620
ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 10680
tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 10740
gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 10800
agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt 10860
actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 10920
catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 10980
gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 11040
cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 11100
gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 11160
aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 11220
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 11280
cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 11340
ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 11400
cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 11460
agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 11520
gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 11580
gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 11640
tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 11700
agaagatccg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg 11760
gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc c 11811
<210>26
<211>11829
<212>DNA
<213>人工
<220>
<223>pTVE468
<400>26
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttattta 540
tccacgacat tgatgttgtg ggccttcaac tcttccttaa ccttattgat gacttcggga 600
tcatcgctga tgggtcttgt gtaatccagc aggacagtgg tcttataacc tagttctgca 660
gcggaaatgg cggtggcttt gacacaatac tccaaagcta caccgacaat gtaaacctcg 720
tctgtatgat gcttttctaa gtacttgttc atgtcggtct tatggaagtt ccagatgtcg 780
tggaaggcgg agtagtattc acggtcagtc aagaaaccct tgtcgacaat cttaatatgc 840
ttagtgacca cttggtccat tatttggtca accaattgac taccccaggt gtttttcaca 900
cagtgtacgg gccacaaaat accctcttgc gtggaatcat cgcctggcct tggagagtgg 960
taggtgtatg ttgaataggg ttctttatct ttatggttct ttgcgaacga aatatgtctg 1020
gaagggtgcc aatctctggt gaccacaatc ctgtgccagt ctctatcagc atcttgcatc 1080
aaatccgaga taggattgat taattcctca ccttttggaa cagtcaagga acctaaaggt 1140
gaaataaaat cattttgcat atcaacaacc ttgcgcttct tcttgggaat taaagtctcc 1200
atggttttgg tttaataaga agagaaaaga gttcttttgt tatggctgaa gtaatagaga 1260
aatgagctcg agtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg 1120
aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt 1380
gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc 1440
catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat 1500
gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag 1560
ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag 1620
ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct 1680
ccaccatgtt gacgaagatt ttcttcttgt cattgagtcg taaaagactc tgtatgaact 1740
gttcgccagt cttcacggcg agttctgtta gatcctcgat ctgaattttt gactccatgt 1800
atggtgcata tggcgcgcca tatgcccggg ccctgtacag cggccgcgtt aacgcgtata 1860
ctctagagcg atcgcccggg ccggccattt aaatgaattc gagctcggta cccaaacgcg 1920
gccgcaagct ataacttcgt atagcataca ttatacgaag ttattcgact ctagaggatc 1980
ccaattccca tgcatggagt caaagattca aatagaggac acttctcgaa ctcggccgtc 2040
gaactcggcc gtcgagtaca tggtcgataa gaaaaggcaa tttgtagatg ttaattccca 2100
tcttgaaaga aatatagttt aaatatttat tgataaaata acaagtcagg tattatagtc 2160
caagcaaaaa cataaattta ttgatgcaag tttaaattca gaaatatttc aataactgat 2220
tatatcagct ggtacattgc cgtagatgaa agactgagtg cgatattatg tgtaatacat 2280
aaattgatga tatagctagc ttagctcatc gggggatcct agacgcgtga gatcagatct 2340
cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac 2400
ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat 2460
atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca 2520
cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca 2580
gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg 2640
cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg 2700
cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt 2760
cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc 2820
agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggt 2880
ccattgttct tctttactct ttgtgtgact gaggtttggt ctagtgcttt ggtcatctat 2940
atataatgat aacaacaatg agaacaagct ttggagtgat cggagggtct aggatacatg 3000
agattcaagt ggactaggat ctacaccgtt ggattttgag tgtggatatg tgtgaggtta 3060
attttacttg gtaacggcca caaaggccta aggagaggtg ttgagaccct tatcggcttg 3120
aaccgctgga ataatgccac gtggaagata attccatgaa tcttatcgtt atctatgagt 3180
gaaattgtgt gatggtggag tggtgcttgc tcattttact tgcctggtgg acttggccct 3240
ttccttatgg ggaatttata ttttacttac tatagagctt tcataccttt tttttacctt 3300
ggatttagtt aatatataat ggtatgattc atgaataaaa atgggaaatt tttgaatttg 3360
tactgctaaa tgcataagat taggtgaaac tgtggaatat atattttttt catttaaaag 3420
caaaatttgc cttttactag aattataaat atagaaaaat atataacatt caaataaaaa 3480
tgaaaataag aactttcaaa aaacagaact atgtttaatg tgtaaagatt agtcgcacat 3540
caagtcatct gttacaatat gttacaacaa gtcataagcc caacaaagtt agcacgtcta 3600
aataaactaa agagtccacg aaaatattac aaatcataag cccaacaaag ttattgatca 3660
aaaaaaaaaa acgcccaaca aagctaaaca aagtccaaaa aaaacttctc aagtctccat 3720
cttcctttat gaacattgaa aactatacac aaaacaagtc agataaatct ctttctgggc 3780
ctgtcttccc aacctcctac atcacttccc tatcggattg aatgttttac ttgtaccttt 3840
tccgttgcaa tgatattgat agtatgtttg tgaaaactaa tagggttaac aatcgaagtc 3900
atggaatatg gatttggtcc aagattttcc gagagctttc tagtagaaag cccatcacca 3960
gaaatttact agtaaaataa atcaccaatt aggtttctta ttatgtgcca aattcaatat 4020
aattatagag gatatttcaa atgaaaacgt atgaatgtta ttagtaaatg gtcaggtaag 4080
acattaaaaa aatcctacgt cagatattca actttaaaaa ttcgatcagt gtggaattgt 4140
acaaaaattt gggatctact atatatatat aatgctttac aacacttgga tttttttttg 4200
gaggctggaa tttttaatct acatatttgt tttggccatg caccaactca ttgtttagtg 4260
taatactttg attttgtcaa atatatgtgt tcgtgtatat ttgtataaga atttctttga 4320
ccatatacac acacacatat atatatatat atatatatta tatatcatgc acttttaatt 4380
gaaaaaataa tatatatata tatagtgcat tttttctaac aaccatatat gttgcgattg 4440
atctgcaaaa atactgctag agtaatgaaa aatataatct attgctgaaa ttatctcaga 4500
tgttaagatt ttcttaaagt aaattctttc aaattttagc taaaagtctt gtaataacta 4560
aagaataata cacaatctcg accacggaaa aaaaacacat aataaatttg aatttcgacc 4620
gcggtacccg gaattgggtt ataattacct caggtcgagg aattaattcg gtacgtacct 4680
aataacttcg tatagcatac attatacgaa gttatatgga tctcgaggca ttacggcatt 4740
acggcactcg cgagggtccc aattcgagca tggagccatt tacaattgaa tatatcctgc 4800
cgccgctgcc gctttgcacc cggtggagct tgcatgttgg tttctacgca gaactgagcc 4860
ggttaggcag ataatttcca ttgagaactg agccatgtgc accttccccc caacacggtg 4920
agcgacgggg caacggagtg atccacatgg gacttttaaa catcatccgt cggatggcgt 4980
tgcgagagaa gcagtcgatc cgtgagatca gccgacgcac cgggcaggcg cgcaacacga 5040
tcgcaaagta tttgaacgca ggtacaatcg agccgacgtt cacggtaccg gaacgaccaa 5100
gcaagctagc ttagtaaagc cctcgctaga ttttaatgcg gatgttgcga ttacttcgcc 5160
aactattgcg ataacaagaa aaagccagcc tttcatgata tatctcccaa tttgtgtagg 5220
gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag 5280
caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc 5340
ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt 5400
ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat 5460
aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc 5520
agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg 5580
acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg 5640
ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct 5700
ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca 5760
gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt 5820
ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa 5880
caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca 5940
aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca 6000
gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta 6060
cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag 6120
tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctttgtttta gggcgactgc 6180
cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 6240
cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca 6300
tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc 6360
gtgagcgcat acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg 6420
ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag 6480
tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc 6540
aggcattggc ggccttgctg ttcttctacg gcaagtgctg tgcacggatc tgccctggct 6600
tcaggagatc ggaagacctc ggccgtccgg gcgcttgccg gtggtgctga ccccggatga 6660
agtctctaga gctctagagg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt 6720
tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca 6780
aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc tccaaggatc 6840
gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcaggga tcgatccaac 6900
ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg 6960
acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt 7020
ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt 7080
acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg 7140
accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt 7200
ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc 7260
tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg 7320
acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag 7380
agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca 7440
ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca 7500
aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg 7560
cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg 7620
tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg 7680
ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg 7740
ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc 7800
gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc 7860
gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa 7920
gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag 7980
gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg 8040
agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa 8100
ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg 8160
gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg 8220
cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg 8280
aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg 8340
gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac 8400
gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat 8460
ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt 8520
gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag 8580
cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag 8640
ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga 8700
gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga 8760
gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc 8820
gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc 8880
aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga 8940
gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa 9000
gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca 9060
ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag 9120
gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 9180
tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga 9240
agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 9300
accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 9360
ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 9420
ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 9480
tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta 9540
cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 9600
gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 9660
ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 9720
caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 9780
atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 9840
ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 9900
cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 9960
tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 10020
gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 10080
gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 10140
cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 10200
atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 10260
ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 10320
gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 10380
aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 10440
aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 10500
gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 10560
cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 10620
cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg 10680
gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 10740
aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 10800
ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 10860
ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 10920
cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 10980
ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 11040
cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 11100
gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 11160
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 11220
ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 11280
atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 11340
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 11400
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 11460
cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 11520
cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 11580
tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 11640
cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 11700
cagaaaaaaa ggatctcaag aagatccgga aaacgcaagc gcaaagagaa agcaggtagc 11760
ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc 11820
ggaattgcc 11829
<210>27
<211>12393
<212>DNA
<213>人工
<220>
<223>pTVE469
<400>27
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttatgct 540
atagggaggc ataattgatg cttggcttga ataacagcat cacgatcacc agtatttttc 600
atgatatcgt cactaatctt cactgcttta gttccttcag cggaaaaaag cttaataaca 660
atattcatag gtttgctcac ttcactggga ttggaaactt tttggaagtc gctggtaaga 720
tttgtgccga tgccaaatgc agacttaatg ccacatttct cgcagtattt gtacagttcg 780
atacatctgt caacatttaa agcatcgcta tgaacaatta cttttgtgga aggatcgaca 840
cctattgatt tatagtgctt tacgactttt tcaatgtatt cctcagcaca accgctatct 900
tgacgaacac catggaaaac attggctaaa tcgtcggcag aattggctgt aaaagatttg 960
agaaacacat cagtagagaa tgtatccgtt aaggctatta aaagactagt accaaaagtt 1020
tggacccact ttaaggaagc aatacgattt gcttgtttat aattttgagt aatagctgca 1080
atgcccatat accactcgtg agcaaccgta ccagagacat ttagattata tttggcggcg 1140
aagtaaacat tagatgtacc aaggaaactt ccagggccct taaaatcctc ttgtgctttc 1200
atgagacctt ggagaacaat ttcctgggtg tgaggatcac gacgacgacg agtgccaaag 1260
tcagtaaagg cacatccggc tcggatgaga cgcttaccct tctcgtaagc tttttcaaac 1320
tgaccctcag gtgaccagtc cttatcgaca aatttaaaat aagattctga gacgagagca 1380
agcagtggaa tttcataaaa aatggtattc ttccagaggc cgtgaataaa gattgagaga 1440
tccttagttt cagaatcata attaagggaa attgaatttt caggatcaaa ttcgaactca 1500
tgcatgaatt cataaaatga ttcctttaaa taaggacagt tcttgcgaag ccattgctct 1560
tcttcaggaa gtaaatgtaa attccgtaag cctcttattt gttcccgtaa ccagttataa 1620
gcctcctgat ttaatgccat ttttggggac cggtttgtat acttatatga tacttgagca 1680
tccggataat gctctaaaac ggcttgaagc atggtgagtt tgtaaagatc cgtatcgagg 1740
atagagacaa cagccggttc acccatggtt ttggtttaat aagaagagaa aagagttctt 1800
ttgttatggc tgaagtaata gagaaatgag ctcgagtcct ctccaaatga aatgaacttc 1860
cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag 1920
tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca 1980
cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa 2040
cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg 2100
tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta 2160
ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct 2220
tggagtagac gagagtgtcg tgctccacca tgttgacgaa gattttcttc ttgtcattga 2280
gtcgtaaaag actctgtatg aactgttcgc cagtcttcac ggcgagttct gttagatcct 2340
cgatctgaat ttttgactcc atgtatggtg catatggcgc gccatatgcc cgggccctgt 2400
acagcggccg cgttaacgcg tatactctag agcgatcgcc cgggccggcc atttaaatga 2460
attcgagctc ggtacccaaa cgcggccgca agctataact tcgtatagca tacattatac 2520
gaagttattc gactctagag gatcccaatt cccatgcatg gagtcaaaga ttcaaataga 2580
ggacacttct cgaactcggc cgtcgaactc ggccgtcgag tacatggtcg ataagaaaag 2640
gcaatttgta gatgttaatt cccatcttga aagaaatata gtttaaatat ttattgataa 2700
aataacaagt caggtattat agtccaagca aaaacataaa tttattgatg caagtttaaa 2760
ttcagaaata tttcaataac tgattatatc agctggtaca ttgccgtaga tgaaagactg 2820
agtgcgatat tatgtgtaat acataaattg atgatatagc tagcttagct catcggggga 2880
tcctagacgc gtgagatcag atctcggtga cgggcaggac cggacggggc ggtaccggca 2940
ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg 3000
cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt 3060
tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca 3120
ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg 3180
tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc 3240
cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga 3300
ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct 3360
cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga 3420
tgtcggccgg gcgtcgttct gggtccattg ttcttcttta ctctttgtgt gactgaggtt 3480
tggtctagtg ctttggtcat ctatatataa tgataacaac aatgagaaca agctttggag 3540
tgatcggagg gtctaggata catgagattc aagtggacta ggatctacac cgttggattt 3600
tgagtgtgga tatgtgtgag gttaatttta cttggtaacg gccacaaagg cctaaggaga 3660
ggtgttgaga cccttatcgg cttgaaccgc tggaataatg ccacgtggaa gataattcca 3720
tgaatcttat cgttatctat gagtgaaatt gtgtgatggt ggagtggtgc ttgctcattt 3780
tacttgcctg gtggacttgg ccctttcctt atggggaatt tatattttac ttactataga 3840
gctttcatac ctttttttta ccttggattt agttaatata taatggtatg attcatgaat 3900
aaaaatggga aatttttgaa tttgtactgc taaatgcata agattaggtg aaactgtgga 3960
atatatattt ttttcattta aaagcaaaat ttgcctttta ctagaattat aaatatagaa 4020
aaatatataa cattcaaata aaaatgaaaa taagaacttt caaaaaacag aactatgttt 4080
aatgtgtaaa gattagtcgc acatcaagtc atctgttaca atatgttaca acaagtcata 4140
agcccaacaa agttagcacg tctaaataaa ctaaagagtc cacgaaaata ttacaaatca 4200
taagcccaac aaagttattg atcaaaaaaa aaaaacgccc aacaaagcta aacaaagtcc 4260
aaaaaaaact tctcaagtct ccatcttcct ttatgaacat tgaaaactat acacaaaaca 4320
agtcagataa atctctttct gggcctgtct tcccaacctc ctacatcact tccctatcgg 4380
attgaatgtt ttacttgtac cttttccgtt gcaatgatat tgatagtatg tttgtgaaaa 4440
ctaatagggt taacaatcga agtcatggaa tatggatttg gtccaagatt ttccgagagc 4500
tttctagtag aaagcccatc accagaaatt tactagtaaa ataaatcacc aattaggttt 4560
cttattatgt gccaaattca atataattat agaggatatt tcaaatgaaa acgtatgaat 4620
gttattagta aatggtcagg taagacatta aaaaaatcct acgtcagata ttcaacttta 4680
aaaattcgat cagtgtggaa ttgtacaaaa atttgggatc tactatatat atataatgct 4740
ttacaacact tggatttttt tttggaggct ggaattttta atctacatat ttgttttggc 4800
catgcaccaa ctcattgttt agtgtaatac tttgattttg tcaaatatat gtgttcgtgt 4860
atatttgtat aagaatttct ttgaccatat acacacacac atatatatat atatatatat 4920
attatatatc atgcactttt aattgaaaaa ataatatata tatatatagt gcattttttc 4980
taacaaccat atatgttgcg attgatctgc aaaaatactg ctagagtaat gaaaaatata 5040
atctattgct gaaattatct cagatgttaa gattttctta aagtaaattc tttcaaattt 5100
tagctaaaag tcttgtaata actaaagaat aatacacaat ctcgaccacg gaaaaaaaac 5160
acataataaa tttgaatttc gaccgcggta cccggaattg ggttataatt acctcaggtc 5220
gaggaattaa ttcggtacgt acctaataac ttcgtatagc atacattata cgaagttata 5280
tggatctcga ggcattacgg cattacggca ctcgcgaggg tcccaattcg agcatggagc 5340
catttacaat tgaatatatc ctgccgccgc tgccgctttg cacccggtgg agcttgcatg 5400
ttggtttcta cgcagaactg agccggttag gcagataatt tccattgaga actgagccat 5460
gtgcaccttc cccccaacac ggtgagcgac ggggcaacgg agtgatccac atgggacttt 5520
taaacatcat ccgtcggatg gcgttgcgag agaagcagtc gatccgtgag atcagccgac 5580
gcaccgggca ggcgcgcaac acgatcgcaa agtatttgaa cgcaggtaca atcgagccga 5640
cgttcacggt accggaacga ccaagcaagc tagcttagta aagccctcgc tagattttaa 5700
tgcggatgtt gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat 5760
gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac 5820
ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa 5880
gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct 5940
tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca 6000
agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact 6060
gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg 6120
ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc 6180
agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt 6240
caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc 6300
ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg 6360
caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc 6420
acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct 6480
ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat 6540
caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat 6600
ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga 6660
cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt 6720
ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca 6780
aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc 6840
caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt 6900
cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa 6960
ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc 7020
cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca 7080
aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagt 7140
gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt ccgggcgctt 7200
gccggtggtg ctgaccccgg atgaagtctc tagagctcta gagggttcgc atcctcggtt 7260
ttctggaagg cgagcatcgt ttgttcgccc agcttctgta tggaacgggc atgcggatca 7320
gtgagggttt gcaactgcgg gtcaaggatc tggatttcga tcacggcacg atcatcgtgc 7380
gggagggcaa gggctccaag gatcgggcct tgatgttacc cgagagcttg gcacccagcc 7440
tgcgcgagca gggatcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt 7500
cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg 7560
ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata 7620
cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg 7680
gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg 7740
cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg 7800
agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag 7860
accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg 7920
gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca 7980
tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca 8040
cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc 8100
tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga 8160
aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca 8220
gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga 8280
ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc 8340
gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat 8400
cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat 8460
cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga 8520
aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc 8580
ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag 8640
gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta 8700
gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc 8760
agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac 8820
cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac 8880
ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg 8940
attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt 9000
aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg 9060
atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc 9120
attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca 9180
accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct 9240
gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa 9300
acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc 9360
tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca 9420
agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca 9480
tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct 9540
aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg 9600
tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat 9660
ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat ccggcccggt 9720
acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc 9780
ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga 9840
tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc 9900
gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg 9960
cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc 10020
tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc 10080
cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga 10140
atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca 10200
cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct 10260
ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc 10320
caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat 10380
cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta 10440
ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt 10500
gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc 10560
agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa 10620
gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt 10680
gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga 10740
gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc 10800
aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa 10860
gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg 10920
gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc 10980
tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg 11040
cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc 11100
cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg 11160
caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca 11220
aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 11280
cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 11340
cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 11400
gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 11460
gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 11520
ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 11580
ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 11640
agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 11700
taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 11760
cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 11820
tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 11880
gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 11940
gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 12000
tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 12060
gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 12120
cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 12180
aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 12240
tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc cggaaaacgc 12300
aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc 12360
ggttttatgg acagcaagcg aaccggaatt gcc 12393
<210>28
<211>12414
<212>DNA
<213>人工
<220>
<223>pTVE 470
<400>28
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcttatgct 540
atagggaggc ataattgatg cttggcttga ataacagcat cacgatcacc agtatttttc 600
atgatatcgt cactaatctt cactgcttta gttccttcag cggaaaaaag cttaataaca 660
atattcatag gtttgctcac ttcactggga ttggaaactt tttggaagtc gctggtaaga 720
tttgtgccga tgccaaatgc agacttaatg ccacatttct cgcagtattt gtacagttcg 780
atacatctgt caacatttaa agcatcgcta tgaacaatta cttttgtgga aggatcgaca 840
cctattgatt tatagtgctt tacgactttt tcaatgtatt cctcagcaca accgctatct 900
tgacgaacac catggaaaac attggctaaa tcgtcggcag aattggctgt aaaagatttg 960
agaaacacat cagtagagaa tgtatccgtt aaggctatta aaagactagt accaaaagtt 1020
tggacccact ttaaggaagc aatacgattt gcttgtttat aattttgagt aatagctgca 1080
atgcccatat accactcgtg agcaaccgta ccagagacat ttagattata tttggcggcg 1140
aagtaaacat tagatgtacc aaggaaactt ccagggccct taaaatcctc ttgtgctttc 1200
atgagacctt ggagaacaat ttcctgggtg tgaggatcac gacgacgacg agtgccaaag 1260
tcagtaaagg cacatccggc tcggatgaga cgcttaccct tctcgtaagc tttttcaaac 1320
tgaccctcag gtgaccagtc cttatcgaca aatttaaaat aagattctga gacgagagca 1380
agcagtggaa tttcataaaa aatggtattc ttccagaggc cgtgaataaa gattgagaga 1440
tccttagttt cagaatcata attaagggaa attgaatttt caggatcaaa ttcgaactca 1500
tgcatgaatt cataaaatga ttcctttaaa taaggacagt tcttgcgaag ccattgctct 1560
tcttcaggaa gtaaatgtaa attccgtaag cctcttattt gttcccgtaa ccagttataa 1620
gcctcctgat ttaatgccat ttttggggac cggtttgtat acttatatga tacttgagca 1680
tccggataat gctctaaaac ggcttgaagc atggtgagtt tgtaaagatc cgtatcgagg 1740
atagagacaa cagcaacctt gcgcttcttc ttgggcggtt cacccatggt tttggtttaa 1800
taagaagaga aaagagttct tttgttatgg ctgaagtaat agagaaatga gctcgagtcc 1860
tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt 1920
gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg 1980
ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac 2040
tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg 2100
tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc 2160
cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg 2220
agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacga 2280
agattttctt cttgtcattg agtcgtaaaa gactctgtat gaactgttcg ccagtcttca 2340
cggcgagttc tgttagatcc tcgatctgaa tttttgactc catgtatggt gcatatggcg 2400
cgccatatgc ccgggccctg tacagcggcc gcgttaacgc gtatactcta gagcgatcgc 2460
ccgggccggc catttaaatg aattcgagct cggtacccaa acgcggccgc aagctataac 2520
ttcgtatagc atacattata cgaagttatt cgactctaga ggatcccaat tcccatgcat 2580
ggagtcaaag attcaaatag aggacacttc tcgaactcgg ccgtcgaact cggccgtcga 2640
gtacatggtc gataagaaaa ggcaatttgt agatgttaat tcccatcttg aaagaaatat 2700
agtttaaata tttattgata aaataacaag tcaggtatta tagtccaagc aaaaacataa 2760
atttattgat gcaagtttaa attcagaaat atttcaataa ctgattatat cagctggtac 2820
attgccgtag atgaaagact gagtgcgata ttatgtgtaa tacataaatt gatgatatag 2880
ctagcttagc tcatcggggg atcctagacg cgtgagatca gatctcggtg acgggcagga 2940
ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt 3000
tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt 3060
gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct 3120
gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt 3180
ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc 3240
aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat 3300
agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac 3360
ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt 3420
ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggtccatt gttcttcttt 3480
actctttgtg tgactgaggt ttggtctagt gctttggtca tctatatata atgataacaa 3540
caatgagaac aagctttgga gtgatcggag ggtctaggat acatgagatt caagtggact 3600
aggatctaca ccgttggatt ttgagtgtgg atatgtgtga ggttaatttt acttggtaac 3660
ggccacaaag gcctaaggag aggtgttgag acccttatcg gcttgaaccg ctggaataat 3720
gccacgtgga agataattcc atgaatctta tcgttatcta tgagtgaaat tgtgtgatgg 3780
tggagtggtg cttgctcatt ttacttgcct ggtggacttg gccctttcct tatggggaat 3840
ttatatttta cttactatag agctttcata cctttttttt accttggatt tagttaatat 3900
ataatggtat gattcatgaa taaaaatggg aaatttttga atttgtactg ctaaatgcat 3960
aagattaggt gaaactgtgg aatatatatt tttttcattt aaaagcaaaa tttgcctttt 4020
actagaatta taaatataga aaaatatata acattcaaat aaaaatgaaa ataagaactt 4080
tcaaaaaaca gaactatgtt taatgtgtaa agattagtcg cacatcaagt catctgttac 4140
aatatgttac aacaagtcat aagcccaaca aagttagcac gtctaaataa actaaagagt 4200
ccacgaaaat attacaaatc ataagcccaa caaagttatt gatcaaaaaa aaaaaacgcc 4260
caacaaagct aaacaaagtc caaaaaaaac ttctcaagtc tccatcttcc tttatgaaca 4320
ttgaaaacta tacacaaaac aagtcagata aatctctttc tgggcctgtc ttcccaacct 4380
cctacatcac ttccctatcg gattgaatgt tttacttgta ccttttccgt tgcaatgata 4440
ttgatagtat gtttgtgaaa actaataggg ttaacaatcg aagtcatgga atatggattt 4500
ggtccaagat tttccgagag ctttctagta gaaagcccat caccagaaat ttactagtaa 4560
aataaatcac caattaggtt tcttattatg tgccaaattc aatataatta tagaggatat 4620
ttcaaatgaa aacgtatgaa tgttattagt aaatggtcag gtaagacatt aaaaaaatcc 4680
tacgtcagat attcaacttt aaaaattcga tcagtgtgga attgtacaaa aatttgggat 4740
ctactatata tatataatgc tttacaacac ttggattttt ttttggaggc tggaattttt 4800
aatctacata tttgttttgg ccatgcacca actcattgtt tagtgtaata ctttgatttt 4860
gtcaaatata tgtgttcgtg tatatttgta taagaatttc tttgaccata tacacacaca 4920
catatatata tatatatata tattatatat catgcacttt taattgaaaa aataatatat 4980
atatatatag tgcatttttt ctaacaacca tatatgttgc gattgatctg caaaaatact 5040
gctagagtaa tgaaaaatat aatctattgc tgaaattatc tcagatgtta agattttctt 5100
aaagtaaatt ctttcaaatt ttagctaaaa gtcttgtaat aactaaagaa taatacacaa 5160
tctcgaccac ggaaaaaaaa cacataataa atttgaattt cgaccgcggt acccggaatt 5220
gggttataat tacctcaggt cgaggaatta attcggtacg tacctaataa cttcgtatag 5280
catacattat acgaagttat atggatctcg aggcattacg gcattacggc actcgcgagg 5340
gtcccaattc gagcatggag ccatttacaa ttgaatatat cctgccgccg ctgccgcttt 5400
gcacccggtg gagcttgcat gttggtttct acgcagaact gagccggtta ggcagataat 5460
ttccattgag aactgagcca tgtgcacctt ccccccaaca cggtgagcga cggggcaacg 5520
gagtgatcca catgggactt ttaaacatca tccgtcggat ggcgttgcga gagaagcagt 5580
cgatccgtga gatcagccga cgcaccgggc aggcgcgcaa cacgatcgca aagtatttga 5640
acgcaggtac aatcgagccg acgttcacgg taccggaacg accaagcaag ctagcttagt 5700
aaagccctcg ctagatttta atgcggatgt tgcgattact tcgccaacta ttgcgataac 5760
aagaaaaagc cagcctttca tgatatatct cccaatttgt gtagggctta ttatgcacgc 5820
ttaaaaataa taaaagcaga cttgacctga tagtttggct gtgagcaatt atgtgcttag 5880
tgcatctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta 5940
gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca 6000
actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt 6060
caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat 6120
ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta 6180
catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta 6240
gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta 6300
ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg 6360
tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt 6420
cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta 6480
cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca 6540
aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac 6600
tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg 6660
gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga 6720
tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat 6780
cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg 6840
cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg 6900
cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct 6960
acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc 7020
gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt 7080
cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct 7140
tgctgttctt ctacggcaag tgctgtgcac ggatctgccc tggcttcagg agatcggaag 7200
acctcggccg tccgggcgct tgccggtggt gctgaccccg gatgaagtct ctagagctct 7260
agagggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc cagcttctgt 7320
atggaacggg catgcggatc agtgagggtt tgcaactgcg ggtcaaggat ctggatttcg 7380
atcacggcac gatcatcgtg cgggagggca agggctccaa ggatcgggcc ttgatgttac 7440
ccgagagctt ggcacccagc ctgcgcgagc agggatcgat ccaacccctc cgctgctata 7500
gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt 7560
cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt 7620
ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga 7680
gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca 7740
accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg 7800
gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg 7860
ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg 7920
ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca 7980
ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc 8040
gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga 8100
agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg 8160
accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc 8220
tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg 8280
ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc 8340
aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga 8400
agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc 8460
aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc 8520
ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga 8580
gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat 8640
acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag 8700
acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta 8760
gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg 8820
ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg 8880
cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc 8940
aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc 9000
gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg 9060
gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg 9120
ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca 9180
ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc 9240
gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag 9300
agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca 9360
aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt 9420
tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta 9480
ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg 9540
agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc 9600
gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg 9660
gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg 9720
tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa 9780
gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga 9840
atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg 9900
tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat 9960
gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct 10020
gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt 10080
agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat 10140
ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc 10200
cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg 10260
cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc 10320
catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc 10380
cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat 10440
cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac 10500
ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc 10560
acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag 10620
tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa 10680
tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat 10740
gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat 10800
gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag 10860
cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc 10920
aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg 10980
cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg 11040
tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc 11100
gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa 11160
aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact 11220
cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa 11280
acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 11340
gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 11400
cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat 11460
tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 11520
ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 11580
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 11640
taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 11700
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 11760
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 11820
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 11880
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 11940
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 12000
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 12060
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 12120
cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 12180
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 12240
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 12300
tcaagaagat ccggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac 12360
atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgcc 12414
<210>29
<211>12366
<212>DNA
<213>人工
<220>
<223>pTVE496
<400>29
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcattct 540
ttgtttccaa gaacttgctt aacaggttcg gtttggtcca catatagtct atgttcttgg 600
atatacctga tgaccgaatt aggtaacaaa tattgtacag acatggcgcg tctgataaat 660
agacgaactt tcgtggaaga aatatcatta tagatgagtt gcttgatgat aagaatattc 720
cttctatgtt catacataat atcatgggat aacaaaaaag accttacatc agaaccagta 780
cgttcgacaa tcaaacaacc gtaattaccg agaatgtgat gtaaatcggc gtccgcccaa 840
acgtttggtt cacccattga ctctattagg tcaccaccag ccagcaacat tattttcaca 900
ccaatttttt ctccagtaac agtagctaca ccacctctct taatattgat ttcgtgattg 960
aaatgatcca agaccttggc agttcttgtg tatgaaggtt gcaatgactc ccatgcatcc 1020
accatcaacc aagatgaggt tctttcgcag gccaattcac acatacgtac tctatggtag 1080
gatggggcca agccttgctt ttgatagtta tcactaacag gggagtaata tccacctatg 1140
acttcaaacc ttgtttgttc agagattgca tctaaagcca tttcaaacat tcttagatgc 1200
aagtaggtga ttggtgaaaa agacccacat gctactatta ctaacggcag tttatttgga 1260
tccagtaatt ttttcgataa tctgtgtgag gggaattcgt agtcttccaa ggttcttgct 1320
tgacgaacga ttccatgtgg aacttcttct aaatcagcaa tctgatattt ctgaacacct 1380
ctagtttctg attctgtggt tgaatctgaa ccgtatctct ccttggatct aaaatccgcg 1440
tcatcatctt cggaagacac ttctgcggaa agtggttgaa agtcgtcttg gttcaatgga 1500
atatgctgat gttttttatc attgccttct ttacgactgt gatgatggtg atgatgatgc 1560
ttaggatgct ttttcttcct cttaatatta aaaggtgcat ctatagagga attcgcatcg 1620
gctaagacgt atggaataat tggaatagat ttgggaattt tagattccgg gtcgggtgga 1680
ggaatcaatt cctcgtctgc agatggcggt ttgaaatccg gagctcttgt gggatccatg 1740
gttttggttt aataagaaga gaaaagagtt cttttgttat ggctgaagta atagagaaat 1800
gagctcgagt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag 1860
gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct 1920
ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 1980
ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 2040
ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 2100
ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 2160
tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 2220
ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt 2280
cgccagtctt cacggcgagt tctgttagat cctcgatctg aatttttgac tccatgtatg 2340
gtgcatatgg cgcgccatat gcccgggccc tgtacagcgg ccgcgttaac gcgtatactc 2400
tagagcgatc gcccgggccg gccatttaaa tgaattcgag ctcggtaccc aaacgcggcc 2460
gcaagctata acttcgtata gcatacatta tacgaagtta ttcgactcta gaggatccca 2520
attcccatgc atggagtcaa agattcaaat agaggacact tctcgaactc ggccgtcgaa 2580
ctcggccgtc gagtacatgg tcgataagaa aaggcaattt gtagatgtta attcccatct 2640
tgaaagaaat atagtttaaa tatttattga taaaataaca agtcaggtat tatagtccaa 2700
gcaaaaacat aaatttattg atgcaagttt aaattcagaa atatttcaat aactgattat 2760
atcagctggt acattgccgt agatgaaaga ctgagtgcga tattatgtgt aatacataaa 2820
ttgatgatat agctagctta gctcatcggg ggatcctaga cgcgtgagat cagatctcgg 2880
tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 2940
cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 3000
cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 3060
tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 3120
ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 3180
tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 3240
cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 3300
gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 3360
ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggtcca 3420
ttgttcttct ttactctttg tgtgactgag gtttggtcta gtgctttggt catctatata 3480
taatgataac aacaatgaga acaagctttg gagtgatcgg agggtctagg atacatgaga 3540
ttcaagtgga ctaggatcta caccgttgga ttttgagtgt ggatatgtgt gaggttaatt 3600
ttacttggta acggccacaa aggcctaagg agaggtgttg agacccttat cggcttgaac 3660
cgctggaata atgccacgtg gaagataatt ccatgaatct tatcgttatc tatgagtgaa 3720
attgtgtgat ggtggagtgg tgcttgctca ttttacttgc ctggtggact tggccctttc 3780
cttatgggga atttatattt tacttactat agagctttca tacctttttt ttaccttgga 3840
tttagttaat atataatggt atgattcatg aataaaaatg ggaaattttt gaatttgtac 3900
tgctaaatgc ataagattag gtgaaactgt ggaatatata tttttttcat ttaaaagcaa 3960
aatttgcctt ttactagaat tataaatata gaaaaatata taacattcaa ataaaaatga 4020
aaataagaac tttcaaaaaa cagaactatg tttaatgtgt aaagattagt cgcacatcaa 4080
gtcatctgtt acaatatgtt acaacaagtc ataagcccaa caaagttagc acgtctaaat 4140
aaactaaaga gtccacgaaa atattacaaa tcataagccc aacaaagtta ttgatcaaaa 4200
aaaaaaaacg cccaacaaag ctaaacaaag tccaaaaaaa acttctcaag tctccatctt 4260
cctttatgaa cattgaaaac tatacacaaa acaagtcaga taaatctctt tctgggcctg 4320
tcttcccaac ctcctacatc acttccctat cggattgaat gttttacttg taccttttcc 4380
gttgcaatga tattgatagt atgtttgtga aaactaatag ggttaacaat cgaagtcatg 4440
gaatatggat ttggtccaag attttccgag agctttctag tagaaagccc atcaccagaa 4500
atttactagt aaaataaatc accaattagg tttcttatta tgtgccaaat tcaatataat 4560
tatagaggat atttcaaatg aaaacgtatg aatgttatta gtaaatggtc aggtaagaca 4620
ttaaaaaaat cctacgtcag atattcaact ttaaaaattc gatcagtgtg gaattgtaca 4680
aaaatttggg atctactata tatatataat gctttacaac acttggattt ttttttggag 4740
gctggaattt ttaatctaca tatttgtttt ggccatgcac caactcattg tttagtgtaa 4800
tactttgatt ttgtcaaata tatgtgttcg tgtatatttg tataagaatt tctttgacca 4860
tatacacaca cacatatata tatatatata tatattatat atcatgcact tttaattgaa 4920
aaaataatat atatatatat agtgcatttt ttctaacaac catatatgtt gcgattgatc 4980
tgcaaaaata ctgctagagt aatgaaaaat ataatctatt gctgaaatta tctcagatgt 5040
taagattttc ttaaagtaaa ttctttcaaa ttttagctaa aagtcttgta ataactaaag 5100
aataatacac aatctcgacc acggaaaaaa aacacataat aaatttgaat ttcgaccgcg 5160
gtacccggaa ttgggttata attacctcag gtcgaggaat taattcggta cgtacctaat 5220
aacttcgtat agcatacatt atacgaagtt atatggatct cgaggcatta cggcattacg 5280
gcactcgcga gggtcccaat tcgagcatgg agccatttac aattgaatat atcctgccgc 5340
cgctgccgct ttgcacccgg tggagcttgc atgttggttt ctacgcagaa ctgagccggt 5400
taggcagata atttccattg agaactgagc catgtgcacc ttccccccaa cacggtgagc 5460
gacggggcaa cggagtgatc cacatgggac ttttaaacat catccgtcgg atggcgttgc 5520
gagagaagca gtcgatccgt gagatcagcc gacgcaccgg gcaggcgcgc aacacgatcg 5580
caaagtattt gaacgcaggt acaatcgagc cgacgttcac ggtaccggaa cgaccaagca 3640
agctagctta gtaaagccct cgctagattt taatgcggat gttgcgatta cttcgccaac 5700
tattgcgata acaagaaaaa gccagccttt catgatatat ctcccaattt gtgtagggct 5760
tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa 5820
ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 5880
aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga 5940
caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag 6000
cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 6060
cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 6120
acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 6180
aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 6240
ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 6300
caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 6360
caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 6420
tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 6480
ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 6540
aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 6600
ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 6660
atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct 6720
gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt 6780
gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga 6840
aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 6900
agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc 6960
gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 7020
aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 7080
cattggcggc cttgctgttc ttctacggca agtgctgtgc acggatctgc cctggcttca 7140
ggagatcgga agacctcggc cgtccgggcg cttgccggtg gtgctgaccc cggatgaagt 7200
ctctagagct ctagagggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg 7260
cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg 7320
atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg 7380
ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagggatcg atccaacccc 7440
tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 7500
tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 7560
ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 7620
ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 7680
aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 7740
agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 7800
gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 7860
tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 7920
cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 7980
ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 8040
cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 8100
gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 8160
atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 8220
ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 8280
agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 8340
tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 8400
cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 8460
ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 8520
atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 8580
aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 8640
gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 8700
gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 8760
gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 8820
gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 8880
gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 8940
atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 9000
aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 9060
gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 9120
gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 9180
gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 9240
aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 9300
cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 9360
tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 9420
gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 9480
aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 9540
caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 9600
gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 9660
tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 9720
cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 9780
acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 9840
gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 9900
tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 9960
cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 10020
agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 10080
cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 10140
gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 10200
gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 10260
cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 10320
cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 10380
gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 10440
ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 10500
ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 10560
ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 10620
gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 10680
gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 10740
tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt 10800
tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 10860
cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 10920
agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 10980
ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc 11040
tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 11100
tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 11160
gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 11220
atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 11280
cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 11340
gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 11400
atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 11460
aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 11520
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 11580
agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 11640
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 11700
caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 11760
gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 11820
cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 11880
tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 11940
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 12000
cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 12060
tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 12120
tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 12180
caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 12240
aaaaaaagga tctcaagaag atccggaaaa cgcaagcgca aagagaaagc aggtagcttg 12300
cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 12360
attgcc 12366
<210>30
<211>12384
<212>DNA
<213>人工
<220>
<223>pTVE497
<400>30
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcattct 540
ttgtttccaa gaacttgctt aacaggttcg gtttggtcca catatagtct atgttcttgg 600
atatacctga tgaccgaatt aggtaacaaa tattgtacag acatggcgcg tctgataaat 660
agacgaactt tcgtggaaga aatatcatta tagatgagtt gcttgatgat aagaatattc 720
cttctatgtt catacataat atcatgggat aacaaaaaag accttacatc agaaccagta 780
cgttcgacaa tcaaacaacc gtaattaccg agaatgtgat gtaaatcggc gtccgcccaa 840
acgtttggtt cacccattga ctctattagg tcaccaccag ccagcaacat tattttcaca 900
ccaatttttt ctccagtaac agtagctaca ccacctctct taatattgat ttcgtgattg 960
aaatgatcca agaccttggc agttcttgtg tatgaaggtt gcaatgactc ccatgcatcc 1020
accatcaacc aagatgaggt tctttcgcag gccaattcac acatacgtac tctatggtag 1080
gatggggcca agccttgctt ttgatagtta tcactaacag gggagtaata tccacctatg 1140
acttcaaacc ttgtttgttc agagattgca tctaaagcca tttcaaacat tcttagatgc 1200
aagtaggtga ttggtgaaaa agacccacat gctactatta ctaacggcag tttatttgga 1260
tccagtaatt ttttcgataa tctgtgtgag gggaattcgt agtcttccaa ggttcttgct 1320
tgacgaacga ttccatgtgg aacttcttct aaatcagcaa tctgatattt ctgaacacct 1380
ctagtttctg attctgtggt tgaatctgaa ccgtatctct ccttggatct aaaatccgcg 1440
tcatcatctt cggaagacac ttctgcggaa agtggttgaa agtcgtcttg gttcaatgga 1500
atatgctgat gttttttatc attgccttct ttacgactgt gatgatggtg atgatgatgc 1560
ttaggatgct ttttcttcct cttaatatta aaaggtgcat ctatagagga attcgcatcg 1620
gctaagacgt atggaataat tggaatagat ttgggaattt tagattccgg gtcgggtgga 1680
ggaatcaatt cctcgtctgc agatggcggt ttgaaatccg gagctcttgt aaccttgcgc 1740
ttcttcttgg gatccatggt tttggtttaa taagaagaga aaagagttct tttgttatgg 1800
ctgaagtaat agagaaatga gctcgagtcc tctccaaatg aaatgaactt ccttatatag 1860
aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat 1920
cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc 1980
tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct 2040
ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga 2100
tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt 2160
gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga 2220
cgagagtgtc gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaaa 2280
gactctgtat gaactgttcg ccagtcttca cggcgagttc tgttagatcc tcgatctgaa 2340
tttttgactc catgtatggt gcatatggcg cgccatatgc ccgggccctg tacagcggcc 2400
gcgttaacgc gtatactcta gagcgatcgc ccgggccggc catttaaatg aattcgagct 2460
cggtacccaa acgcggccgc aagctataac ttcgtatagc atacattata cgaagttatt 2520
cgactctaga ggatcccaat tcccatgcat ggagtcaaag attcaaatag aggacacttc 2580
tcgaactcgg ccgtcgaact cggccgtcga gtacatggtc gataagaaaa ggcaatttgt 2640
agatgttaat tcccatcttg aaagaaatat agtttaaata tttattgata aaataacaag 2700
tcaggtatta tagtccaagc aaaaacataa atttattgat gcaagtttaa attcagaaat 2760
atttcaataa ctgattatat cagctggtac attgccgtag atgaaagact gagtgcgata 2820
ttatgtgtaa tacataaatt gatgatatag ctagcttagc tcatcggggg atcctagacg 2880
cgtgagatca gatctcggtg acgggcagga ccggacgggg cggtaccggc aggctgaagt 2940
ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca 3000
tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc 3060
cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt 3120
agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg 3180
ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct 3240
cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg 3300
tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt 3360
ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg 3420
ggcgtcgttc tgggtccatt gttcttcttt actctttgtg tgactgaggt ttggtctagt 3480
gctttggtca tctatatata atgataacaa caatgagaac aagctttgga gtgatcggag 3540
ggtctaggat acatgagatt caagtggact aggatctaca ccgttggatt ttgagtgtgg 3600
atatgtgtga ggttaatttt acttggtaac ggccacaaag gcctaaggag aggtgttgag 3660
acccttatcg gcttgaaccg ctggaataat gccacgtgga agataattcc atgaatctta 3720
tcgttatcta tgagtgaaat tgtgtgatgg tggagtggtg cttgctcatt ttacttgcct 3780
ggtggacttg gccctttcct tatggggaat ttatatttta cttactatag agctttcata 3840
cctttttttt accttggatt tagttaatat ataatggtat gattcatgaa taaaaatggg 3900
aaatttttga atttgtactg ctaaatgcat aagattaggt gaaactgtgg aatatatatt 3960
tttttcattt aaaagcaaaa tttgcctttt actagaatta taaatataga aaaatatata 4020
acattcaaat aaaaatgaaa ataagaactt tcaaaaaaca gaactatgtt taatgtgtaa 4080
agattagtcg cacatcaagt catctgttac aatatgttac aacaagtcat aagcccaaca 4140
aagttagcac gtctaaataa actaaagagt ccacgaaaat attacaaatc ataagcccaa 4200
caaagttatt gatcaaaaaa aaaaaacgcc caacaaagct aaacaaagtc caaaaaaaac 4260
ttctcaagtc tccatcttcc tttatgaaca ttgaaaacta tacacaaaac aagtcagata 4320
aatctctttc tgggcctgtc ttcccaacct cctacatcac ttccctatcg gattgaatgt 4380
tttacttgta ccttttccgt tgcaatgata ttgatagtat gtttgtgaaa actaataggg 4440
ttaacaatcg aagtcatgga atatggattt ggtccaagat tttccgagag ctttctagta 4500
gaaagcccat caccagaaat ttactagtaa aataaatcac caattaggtt tcttattatg 4560
tgccaaattc aatataatta tagaggatat ttcaaatgaa aacgtatgaa tgttattagt 4620
aaatggtcag gtaagacatt aaaaaaatcc tacgtcagat attcaacttt aaaaattcga 4680
tcagtgtgga attgtacaaa aatttgggat ctactatata tatataatgc tttacaacac 4740
ttggattttt ttttggaggc tggaattttt aatctacata tttgttttgg ccatgcacca 4800
actcattgtt tagtgtaata ctttgatttt gtcaaatata tgtgttcgtg tatatttgta 4860
taagaatttc tttgaccata tacacacaca catatatata tatatatata tattatatat 4920
catgcacttt taattgaaaa aataatatat atatatatag tgcatttttt ctaacaacca 4980
tatatgttgc gattgatctg caaaaatact gctagagtaa tgaaaaatat aatctattgc 5040
tgaaattatc tcagatgtta agattttctt aaagtaaatt ctttcaaatt ttagctaaaa 5100
gtcttgtaat aactaaagaa taatacacaa tctcgaccac ggaaaaaaaa cacataataa 5160
atttgaattt cgaccgcggt acccggaatt gggttataat tacctcaggt cgaggaatta 5220
attcggtacg tacctaataa cttcgtatag catacattat acgaagttat atggatctcg 5280
aggcattacg gcattacggc actcgcgagg gtcccaattc gagcatggag ccatttacaa 5340
ttgaatatat cctgccgccg ctgccgcttt gcacccggtg gagcttgcat gttggtttct 5400
acgcagaact gagccggtta ggcagataat ttccattgag aactgagcca tgtgcacctt 5460
ccccccaaca cggtgagcga cggggcaacg gagtgatcca catgggactt ttaaacatca 5520
tccgtcggat ggcgttgcga gagaagcagt cgatccgtga gatcagccga cgcaccgggc 5580
aggcgcgcaa cacgatcgca aagtatttga acgcaggtac aatcgagccg acgttcacgg 5640
taccggaacg accaagcaag ctagcttagt aaagccctcg ctagatttta atgcggatgt 5700
tgcgattact tcgccaacta ttgcgataac aagaaaaagc cagcctttca tgatatatct 5760
cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga cttgacctga 5820
tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta agccgcgccg 5880
cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct 5940
cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt 6000
cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca 6060
ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc 6120
tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg 6180
gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg 6240
gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg 6300
tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt 6360
cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga 6420
tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg 6480
aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta 6540
cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg 6600
agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta 6660
cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg 6720
ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac 6780
ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac 6840
agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg 6900
ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg 6960
cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct 7020
tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg 7080
tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag tgctgtgcac 7140
ggatctgccc tggcttcagg agatcggaag acctcggccg tccgggcgct tgccggtggt 7200
gctgaccccg gatgaagtct ctagagctct agagggttcg catcctcggt tttctggaag 7260
gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt 7320
tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca 7380
agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc 7440
agggatcgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 7500
cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 7560
ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 7620
agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 7680
gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 7740
gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 7800
ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 7860
cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 7920
tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 7980
ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 8040
ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 8100
cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 8160
ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 8220
tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 8280
acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 8340
cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 8400
gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 8460
tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg 8520
ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 8580
gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 8640
gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 8700
ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 8760
gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 8820
attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 8880
caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 8940
cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 9000
attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 9060
acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 9120
tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 9180
gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 9240
tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 9300
gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 9360
cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 9420
tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 9480
taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 9540
ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 9600
acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 9660
acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg 9720
cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 9780
acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 9840
caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 9900
cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 9960
cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 10020
gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 10080
cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 10140
ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 10200
cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 10260
ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 10320
ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 10380
cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 10440
cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 10500
cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 10560
atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 10620
tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 10680
ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 10740
atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 10800
aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 10860
tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 10920
gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 10980
tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 11040
agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 11100
tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 11160
agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct 11220
gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 11280
tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 11340
gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 11400
ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 11460
aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 11520
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 11580
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 11640
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 11700
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 11760
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 11820
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 11880
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 11940
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 12000
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 12060
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 12120
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 12180
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 12240
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat ccggaaaacg caagcgcaaa 12300
gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg 12360
gacagcaagc gaaccggaat tgcc 12384
<210>31
<211>12348
<212>DNA
<213>人工
<220>
<223>pTVE 500
<400>31
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcactct 540
ttgctatcca agacctgctt gaccggttca ctttgattaa tgtatagatt atactcttgg 600
atgtaacgga tgacagagtt tggaagaaga tattgaactg acattccacg tctgatgaaa 660
agccgcactt tcgtagagga aatatcattg taaataagtt gtttgataat aaggatattt 720
cttctgtgtt catacatgat atcatgggaa agcgagaagg acctaacatc agaaccagtc 780
ctttccacga tcaaacatcc ataattaccc aaaatatggt gcaggtctga atcagcccac 840
acatgaggct cgcccatgga ttcgataaga tcaccgcctg ccaataacat gatttttacg 900
cccatttttt caccatctac agtcatgatt ccacctctct tgatatttat ttcatgattg 960
aaatggtcca agacttttgc tgtccttgta taacttgatt gtaaagattc ccaggcatca 1020
accattaacc aagatgatgt ccgctcgcat gctaattcgc acatgcggac acgatgataa 1080
gctggggcta accctcgctt ttgatagtta tcacttactg gagaaaaata accaccaacc 1140
acttcaaaac gcgtttgctc attgatatca tctaaagcca tttcaaacat tctcaaatgt 1200
aggtatgtta tgggagaaaa tgatccacaa gcaacgatga tcagaggcag tttttcagga 1260
tcttgtaact ttttcgtcaa tctgtgtaca ggaaattcgt aatcttcaat agttctggct 1320
tgtcttacaa ttgtgtgagg aacttcttcc aaatcagcaa tttggctctt cagtaccccc 1380
aaattcccag tcatcgtaac atcctgtaga gtagcgtcaa ttccattcga ttgcccttca 1440
ctttcctcct ccgatgatac atcccgagat agtggctgga agtcactagt atttaatgga 1500
atatggtcca ttcggttgct tgagttggcg ttttttttcc ttgacgacag ggttttgtaa 1560
atgtcgagat tgaaaggagc atcgatcgaa gaattataat cagctaaaac atatggaact 1620
atgggtccag attttggcat cgtatgtgtt ggatctggcg gtggttgtag ttcttcattt 1680
ggctgtggcg gtttaaaatc gggtgctttg gtgggatcca tggttttggt ttaataagaa 1740
gagaaaagag ttcttttgtt atggctgaag taatagagaa atgagctcga gtcctctcca 1800
aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt 1860
catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa 1920
cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg 1980
cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac 2040
cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga 2100
ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg 2160
tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg acgaagattt 2220
tcttcttgtc attgagtcgt aaaagactct gtatgaactg ttcgccagtc ttcacggcga 2280
gttctgttag atcctcgatc tgaatttttg actccatgta tggtgcatat ggcgcgccat 2340
atgcccgggc cctgtacagc ggccgcgtta acgcgtatac tctagagcga tcgcccgggc 2400
cggccattta aatgaattcg agctcggtac ccaaacgcgg ccgcaagcta taacttcgta 2460
tagcatacat tatacgaagt tattcgactc tagaggatcc caattcccat gcatggagtc 2520
aaagattcaa atagaggaca cttctcgaac tcggccgtcg aactcggccg tcgagtacat 2580
ggtcgataag aaaaggcaat ttgtagatgt taattcccat cttgaaagaa atatagttta 2640
aatatttatt gataaaataa caagtcaggt attatagtcc aagcaaaaac ataaatttat 2700
tgatgcaagt ttaaattcag aaatatttca ataactgatt atatcagctg gtacattgcc 2760
gtagatgaaa gactgagtgc gatattatgt gtaatacata aattgatgat atagctagct 2820
tagctcatcg ggggatccta gacgcgtgag atcagatctc ggtgacgggc aggaccggac 2880
ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt 2940
gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc 3000
gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct 3060
ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg 3120
gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc 3180
ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct 3240
cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt 3300
tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct 3360
cggtggcacg gcggatgtcg gccgggcgtc gttctgggtc cattgttctt ctttactctt 3420
tgtgtgactg aggtttggtc tagtgctttg gtcatctata tataatgata acaacaatga 3480
gaacaagctt tggagtgatc ggagggtcta ggatacatga gattcaagtg gactaggatc 3540
tacaccgttg gattttgagt gtggatatgt gtgaggttaa ttttacttgg taacggccac 3600
aaaggcctaa ggagaggtgt tgagaccctt atcggcttga accgctggaa taatgccacg 3660
tggaagataa ttccatgaat cttatcgtta tctatgagtg aaattgtgtg atggtggagt 3720
ggtgcttgct cattttactt gcctggtgga cttggccctt tccttatggg gaatttatat 3780
tttacttact atagagcttt catacctttt ttttaccttg gatttagtta atatataatg 3840
gtatgattca tgaataaaaa tgggaaattt ttgaatttgt actgctaaat gcataagatt 3900
aggtgaaact gtggaatata tatttttttc atttaaaagc aaaatttgcc ttttactaga 3960
attataaata tagaaaaata tataacattc aaataaaaat gaaaataaga actttcaaaa 4020
aacagaacta tgtttaatgt gtaaagatta gtcgcacatc aagtcatctg ttacaatatg 4080
ttacaacaag tcataagccc aacaaagtta gcacgtctaa ataaactaaa gagtccacga 4140
aaatattaca aatcataagc ccaacaaagt tattgatcaa aaaaaaaaaa cgcccaacaa 4200
agctaaacaa agtccaaaaa aaacttctca agtctccatc ttcctttatg aacattgaaa 4260
actatacaca aaacaagtca gataaatctc tttctgggcc tgtcttccca acctcctaca 4320
tcacttccct atcggattga atgttttact tgtacctttt ccgttgcaat gatattgata 4380
gtatgtttgt gaaaactaat agggttaaca atcgaagtca tggaatatgg atttggtcca 4440
agattttccg agagctttct agtagaaagc ccatcaccag aaatttacta gtaaaataaa 4500
tcaccaatta ggtttcttat tatgtgccaa attcaatata attatagagg atatttcaaa 4560
tgaaaacgta tgaatgttat tagtaaatgg tcaggtaaga cattaaaaaa atcctacgtc 4620
agatattcaa ctttaaaaat tcgatcagtg tggaattgta caaaaatttg ggatctacta 4680
tatatatata atgctttaca acacttggat ttttttttgg aggctggaat ttttaatcta 4740
catatttgtt ttggccatgc accaactcat tgtttagtgt aatactttga ttttgtcaaa 4800
tatatgtgtt cgtgtatatt tgtataagaa tttctttgac catatacaca cacacatata 4860
tatatatata tatatattat atatcatgca cttttaattg aaaaaataat atatatatat 4920
atagtgcatt ttttctaaca accatatatg ttgcgattga tctgcaaaaa tactgctaga 4980
gtaatgaaaa atataatcta ttgctgaaat tatctcagat gttaagattt tcttaaagta 5040
aattctttca aattttagct aaaagtcttg taataactaa agaataatac acaatctcga 5100
ccacggaaaa aaaacacata ataaatttga atttcgaccg cggtacccgg aattgggtta 5160
taattacctc aggtcgagga attaattcgg tacgtaccta ataacttcgt atagcataca 5220
ttatacgaag ttatatggat ctcgaggcat tacggcatta cggcactcgc gagggtccca 5280
attcgagcat ggagccattt acaattgaat atatcctgcc gccgctgccg ctttgcaccc 5340
ggtggagctt gcatgttggt ttctacgcag aactgagccg gttaggcaga taatttccat 5400
tgagaactga gccatgtgca ccttcccccc aacacggtga gcgacggggc aacggagtga 5460
tccacatggg acttttaaac atcatccgtc ggatggcgtt gcgagagaag cagtcgatcc 5520
gtgagatcag ccgacgcacc gggcaggcgc gcaacacgat cgcaaagtat ttgaacgcag 5580
gtacaatcga gccgacgttc acggtaccgg aacgaccaag caagctagct tagtaaagcc 5640
ctcgctagat tttaatgcgg atgttgcgat tacttcgcca actattgcga taacaagaaa 5700
aagccagcct ttcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa 5760
ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc 5820
taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt gttagacatt 5880
atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat 5940
ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta 6000
tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg 6060
gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc 6120
gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct 6180
caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg 6240
caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg 6300
gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct 6360
tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc 6420
ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc 6480
gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg 6540
gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga 6600
gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg 6660
cttccctcat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc 6720
tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag 6780
gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt 6840
taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc 6900
attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc 6960
acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc 7020
tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt 7080
tcttctacgg caagtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg 7140
gccgtccggg cgcttgccgg tggtgctgac cccggatgaa gtctctagag ctctagaggg 7200
ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa 7260
cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg 7320
gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga 7380
gcttggcacc cagcctgcgc gagcagggat cgatccaacc cctccgctgc tatagtgcag 7440
tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag 7500
ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc 7560
gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg 7620
ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac 7680
gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca 7740
ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga 7800
cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc 7860
gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca 7920
cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc 7980
taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg 8040
gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg 8100
aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc 8160
gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc 8220
gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg 8280
aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat 8340
cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt 8400
gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag 8460
cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa 8520
cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa 8580
ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc 8640
atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat 8700
tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc 8760
gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac 8820
ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca 8880
gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac 8940
ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt 9000
gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc 9060
gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact 9120
gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc 9180
caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa 9240
tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg 9300
caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg 9360
cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc 9420
tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga 9480
tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc 9540
gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc 9600
tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa 9660
accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa 9720
ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg 9780
gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc 9840
gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta 9900
tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa 9960
gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt 10020
ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt 10080
ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg 10140
cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa 10200
gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca 10260
gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat 10320
tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct 10380
agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca 10440
ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg 10500
cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag 10560
cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct 10620
gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta 10680
ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg 10740
gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta 10800
cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc 10860
gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt 10920
ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata 10980
actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg 11040
ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc 11100
tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg 11160
ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 11220
gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 11280
aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 11340
cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 11400
gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 11460
caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 11520
agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 11580
aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 11640
gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 11700
tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 11760
cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 11820
ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 11880
cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 11940
atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 12000
agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 12060
gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 12120
gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 12180
tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 12240
agatccggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg 12300
atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgcc 12348
<210>32
<211>12366
<212>DNA
<213>人工
<220>
<223>pTVE 501
<400>32
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agctcactct 540
ttgctatcca agacctgctt gaccggttca ctttgattaa tgtatagatt atactcttgg 600
atgtaacgga tgacagagtt tggaagaaga tattgaactg acattccacg tctgatgaaa 660
agccgcactt tcgtagagga aatatcattg taaataagtt gtttgataat aaggatattt 720
cttctgtgtt catacatgat atcatgggaa agcgagaagg acctaacatc agaaccagtc 780
ctttccacga tcaaacatcc ataattaccc aaaatatggt gcaggtctga atcagcccac 840
acatgaggct cgcccatgga ttcgataaga tcaccgcctg ccaataacat gatttttacg 900
cccatttttt caccatctac agtcatgatt ccacctctct tgatatttat ttcatgattg 960
aaatggtcca agacttttgc tgtccttgta taacttgatt gtaaagattc ccaggcatca 1020
accattaacc aagatgatgt ccgctcgcat gctaattcgc acatgcggac acgatgataa 1080
gctggggcta accctcgctt ttgatagtta tcacttactg gagaaaaata accaccaacc 1140
acttcaaaac gcgtttgctc attgatatca tctaaagcca tttcaaacat tctcaaatgt 1200
aggtatgtta tgggagaaaa tgatccacaa gcaacgatga tcagaggcag tttttcagga 1260
tcttgtaact ttttcgtcaa tctgtgtaca ggaaattcgt aatcttcaat agttctggct 1320
tgtcttacaa ttgtgtgagg aacttcttcc aaatcagcaa tttggctctt cagtaccccc 1380
aaattcccag tcatcgtaac atcctgtaga gtagcgtcaa ttccattcga ttgcccttca 1440
ctttcctcct ccgatgatac atcccgagat agtggctgga agtcactagt atttaatgga 1500
atatggtcca ttcggttgct tgagttggcg ttttttttcc ttgacgacag ggttttgtaa 1560
atgtcgagat tgaaaggagc atcgatcgaa gaattataat cagctaaaac atatggaact 1620
atgggtccag attttggcat cgtatgtgtt ggatctggcg gtggttgtag ttcttcattt 1680
ggctgtggcg gtttaaaatc gggtgctttg gtaaccttgc gcttcttctt gggatccatg 1740
gttttggttt aataagaaga gaaaagagtt cttttgttat ggctgaagta atagagaaat 1800
gagctcgagt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag 1860
gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct 1920
ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 1980
ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 2040
ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 2100
ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 2160
tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 2220
ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt 2280
cgccagtctt cacggcgagt tctgttagat cctcgatctg aatttttgac tccatgtatg 2340
gtgcatatgg cgcgccatat gcccgggccc tgtacagcgg ccgcgttaac gcgtatactc 2400
tagagcgatc gcccgggccg gccatttaaa tgaattcgag ctcggtaccc aaacgcggcc 2460
gcaagctata acttcgtata gcatacatta tacgaagtta ttcgactcta gaggatccca 2520
attcccatgc atggagtcaa agattcaaat agaggacact tctcgaactc ggccgtcgaa 2580
ctcggccgtc gagtacatgg tcgataagaa aaggcaattt gtagatgtta attcccatct 2640
tgaaagaaat atagtttaaa tatttattga taaaataaca agtcaggtat tatagtccaa 2700
gcaaaaacat aaatttattg atgcaagttt aaattcagaa atatttcaat aactgattat 2760
atcagctggt acattgccgt agatgaaaga ctgagtgcga tattatgtgt aatacataaa 2820
ttgatgatat agctagctta gctcatcggg ggatcctaga cgcgtgagat cagatctcgg 2880
tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 2940
cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 3000
cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 3060
tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 3120
ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 3180
tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 3240
cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 3300
gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 3360
ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggtcca 3420
ttgttcttct ttactctttg tgtgactgag gtttggtcta gtgctttggt catctatata 3480
taatgataac aacaatgaga acaagctttg gagtgatcgg agggtctagg atacatgaga 3540
ttcaagtgga ctaggatcta caccgttgga ttttgagtgt ggatatgtgt gaggttaatt 3600
ttacttggta acggccacaa aggcctaagg agaggtgttg agacccttat cggcttgaac 3660
cgctggaata atgccacgtg gaagataatt ccatgaatct tatcgttatc tatgagtgaa 3720
attgtgtgat ggtggagtgg tgcttgctca ttttacttgc ctggtggact tggccctttc 3780
cttatgggga atttatattt tacttactat agagctttca tacctttttt ttaccttgga 3840
tttagttaat atataatggt atgattcatg aataaaaatg ggaaattttt gaatttgtac 3900
tgctaaatgc ataagattag gtgaaactgt ggaatatata tttttttcat ttaaaagcaa 3960
aatttgcctt ttactagaat tataaatata gaaaaatata taacattcaa ataaaaatga 4020
aaataagaac tttcaaaaaa cagaactatg tttaatgtgt aaagattagt cgcacatcaa 4080
gtcatctgtt acaatatgtt acaacaagtc ataagcccaa caaagttagc acgtctaaat 4140
aaactaaaga gtccacgaaa atattacaaa tcataagccc aacaaagtta ttgatcaaaa 4200
aaaaaaaacg cccaacaaag ctaaacaaag tccaaaaaaa acttctcaag tctccatctt 4260
cctttatgaa cattgaaaac tatacacaaa acaagtcaga taaatctctt tctgggcctg 4320
tcttcccaac ctcctacatc acttccctat cggattgaat gttttacttg taccttttcc 4380
gttgcaatga tattgatagt atgtttgtga aaactaatag ggttaacaat cgaagtcatg 4440
gaatatggat ttggtccaag attttccgag agctttctag tagaaagccc atcaccagaa 4500
atttactagt aaaataaatc accaattagg tttcttatta tgtgccaaat tcaatataat 4560
tatagaggat atttcaaatg aaaacgtatg aatgttatta gtaaatggtc aggtaagaca 4620
ttaaaaaaat cctacgtcag atattcaact ttaaaaattc gatcagtgtg gaattgtaca 4680
aaaatttggg atctactata tatatataat gctttacaac acttggattt ttttttggag 4740
gctggaattt ttaatctaca tatttgtttt ggccatgcac caactcattg tttagtgtaa 4800
tactttgatt ttgtcaaata tatgtgttcg tgtatatttg tataagaatt tctttgacca 4860
tatacacaca cacatatata tatatatata tatattatat atcatgcact tttaattgaa 4920
aaaataatat atatatatat agtgcatttt ttctaacaac catatatgtt gcgattgatc 4980
tgcaaaaata ctgctagagt aatgaaaaat ataatctatt gctgaaatta tctcagatgt 5040
taagattttc ttaaagtaaa ttctttcaaa ttttagctaa aagtcttgta ataactaaag 5100
aataatacac aatctcgacc acggaaaaaa aacacataat aaatttgaat ttcgaccgcg 5160
gtacccggaa ttgggttata attacctcag gtcgaggaat taattcggta cgtacctaat 5220
aacttcgtat agcatacatt atacgaagtt atatggatct cgaggcatta cggcattacg 5280
gcactcgcga gggtcccaat tcgagcatgg agccatttac aattgaatat atcctgccgc 5340
cgctgccgct ttgcacccgg tggagcttgc atgttggttt ctacgcagaa ctgagccggt 5400
taggcagata atttccattg agaactgagc catgtgcacc ttccccccaa cacggtgagc 5460
gacggggcaa cggagtgatc cacatgggac ttttaaacat catccgtcgg atggcgttgc 5520
gagagaagca gtcgatccgt gagatcagcc gacgcaccgg gcaggcgcgc aacacgatcg 5580
caaagtattt gaacgcaggt acaatcgagc cgacgttcac ggtaccggaa cgaccaagca 5640
agctagctta gtaaagccct cgctagattt taatgcggat gttgcgatta cttcgccaac 5700
tattgcgata acaagaaaaa gccagccttt catgatatat ctcccaattt gtgtagggct 5760
tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa 5820
ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 5880
aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga 5940
caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag 6000
cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 6060
cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 6120
acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 6180
aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 6240
ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 6300
caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 6360
caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 6420
tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 6480
ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 6540
aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 6600
ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 6660
atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct 6720
gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt 6780
gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga 6840
aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 6900
agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc 6960
gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 7020
aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 7080
cattggcggc cttgctgttc ttctacggca agtgctgtgc acggatctgc cctggcttca 7140
ggagatcgga agacctcggc cgtccgggcg cttgccggtg gtgctgaccc cggatgaagt 7200
ctctagagct ctagagggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg 7260
cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg 7320
atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg 7380
ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagggatcg atccaacccc 7440
tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 7500
tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 7560
ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 7620
ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 7680
aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 7740
agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 7800
gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 7860
tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 7920
cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 7980
ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 8040
cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 8100
gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 8160
atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 8220
ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 8280
agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 8340
tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 8400
cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 8460
ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 8520
atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 8580
aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 8640
gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 8700
gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 8760
gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 8820
gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 8880
gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 8940
atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 9000
aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 9060
gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 9120
gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 9180
gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 9240
aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 9300
cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 9360
tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 9420
gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 9480
aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 9540
caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 9600
gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 9660
tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 9720
cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 9780
acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 9840
gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 9900
tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 9960
cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 10020
agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 10080
cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 10140
gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 10200
gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 10260
cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 10320
cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 10380
gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 10440
ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 10500
ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 10560
ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 10620
gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 10680
gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 10740
tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt 10800
tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 10860
cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 10920
agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 10980
ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc 11040
tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 11100
tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 11160
gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 11220
atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 11280
cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 11340
gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 11400
atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 11460
aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 11520
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 11580
agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 11640
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 11700
caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 11760
gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 11820
cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 11880
tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 11940
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 12000
cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 12060
tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 12120
tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 12180
caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 12240
aaaaaaagga tctcaagaag atccggaaaa cgcaagcgca aagagaaagc aggtagcttg 12300
cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 12360
attgcc 12366
<210>33
<211>13305
<212>DNA
<213>人工
<220>
<223>pTVE502
<400>33
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcctaatca 540
atagacataa tgtcaagcgt tgagccttta tgtgcttcac actgctcgac aacttcatca 600
atttttcttg aagcccatgg aaatcttggg ttgattaaga aaggacgtaa gtcaaatctg 660
ttgtcttctg gtgaatactg ttcagcatga taactaggag ttaaaacagt ttgcttgtgt 720
ctgttgatgg catagaagaa gaaaaatctt ttcacctttt cagatatttg acgaggtgtt 780
aactttgggg accattgatg aagaagtttt aagaacatag aataaggacc acatttttca 840
acctttctta ggtaaccaaa cacgcccaat tcttcatacg tcatccccat atctatctca 900
tccgattgaa cgtaatcttt agtcataggt tctaattctg cagttggtgt agcgtttaaa 960
aagtcattca agattggcat gttatattgt tttgatgcgt aggcaatgaa tcttttcaag 1020
tcagtttttg aaataccccc aatagggttg atatctgcgg aggagcagtc atattttgtt 1080
agatacccac gtaagcactc atcaacattt gcgctaccaa gtactaacaa tccacccgag 1140
tttgggatac cacgaaccca cggcaacagt tgcgcaaaaa gataagaaag aaccattctt 1200
agacgcgcct ggatgttttg taaagccaag ttctcgattt gagatccccc aaatattttg 1260
tatattggtt ttttgccagt ggctacttcg aataaggaca ccacactgga taccaatgag 1320
tccatcttta aatccacgtg gtaagatcca attgcattgg aaaggtcctt tgctctgttt 1380
cttgtctcct tggatgaatt ttccgtaccc atgaaacagg agtgaaatat ttttgaggct 1440
agatcctgtg gactgtctgg aatccaatca tcgccgctac gtgttatctt acgaacgtct 1500
ttgataactt gctcatttcc attttgagca gcgtcggtca ctaaacggca catagagtgg 1560
acaatcattg cagttgcaca agagtcaatg cccccagata agggaaggaa aaaccctgtt 1620
ccgttacaac gtcttaaata atcccacatc cagcaagcag gtcccagtgc aatttcttcc 1680
tcaggagagt gatagaaagg ctcgcggact tttgttggac acactgtagg atcaaatctg 1740
gaggtcatta aagccaattc tacaggaata tcaatacgct tgaactttat ttctgccaag 1800
gaggcttgta ggccacgaga catgacagct gcacgataac tcctcacctc ttctaggtcc 1860
acagtagcag taactacttc cacatcatct agcgaaaatt gtgaaccttg ggctacaatt 1920
gtaccattga tggcaattag tgcacagcca tcataatata atctgtcacc atcacaacct 1980
ctttgatttg catacaagta aacaccacca caacgtttag tggcatttaa aattaggtct 2040
aaccttttat ttaacttacg cagttcatga tgagaaccag atgagtttgt catgatttcc 2100
acaccatcta aagacatggc gatgtggggg gattgaggtg taaacaattc ttcacaagtt 2160
tctgtaccaa tgcatgtatc caatgaattt atcacagcgt ccccaaatgg cacaagtctc 2220
tggccggtaa ctttctgaat ctcaggtgga aggataaagt cctccaccac gccaggtttc 2280
atccaaggtg tgaaaaatct catttcccta tagttaccat cattagctaa ccaaatctta 2340
ggtcttatga acaatatctc accatccaag gataacaaac gacaattata acgaacattc 2400
ttgtgtagaa cgggcatacc aatgtcaagt attaatccat gggtttcttt attcttaatg 2460
atttgagcat acatttccca tgaatgaagg caaacgtcat tttctaaaaa atgatctaaa 2520
catccgtagc cagttatttc cagttctggg ccgacacgta acctggcacc cctctctttg 2580
gcaatcttaa tggactgtag gatacggtct ctattacctt caaaatctag ggcccattga 2640
ttcaagttgc atgtagctaa agtgataaga tgtgccatgg ttttggttta ataagaagag 2700
aaaagagttc ttttgttatg gctgaagtaa tagagaaatg agctcgagtc ctctccaaat 2760
gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat 2820
cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt 2880
cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag 2940
aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt 3000
ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt 3060
ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat 3120
ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttgacg aagattttct 3180
tcttgtcatt gagtcgtaaa agactctgta tgaactgttc gccagtcttc acggcgagtt 3240
ctgttagatc ctcgatctga atttttgact ccatgtatgg tgcatatggc gcgccatatg 3300
cccgggccct gtacagcggc cgcgttaacg cgtatactct agagcgatcg cccgggccgg 3360
ccatttaaat gaattcgagc tcggtaccca aacgcggccg caagctataa cttcgtatag 3420
catacattat acgaagttat tcgactctag aggatcccaa ttcccatgca tggagtcaaa 3480
gattcaaata gaggacactt ctcgaactcg gccgtcgaac tcggccgtcg agtacatggt 3540
cgataagaaa aggcaatttg tagatgttaa ttcccatctt gaaagaaata tagtttaaat 3600
atttattgat aaaataacaa gtcaggtatt atagtccaag caaaaacata aatttattga 3660
tgcaagttta aattcagaaa tatttcaata actgattata tcagctggta cattgccgta 3720
gatgaaagac tgagtgcgat attatgtgta atacataaat tgatgatata gctagcttag 3780
ctcatcgggg gatcctagac gcgtgagatc agatctcggt gacgggcagg accggacggg 3840
gcggtaccgg caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct 3900
tgaagccggc cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca 3960
cgctcgggtc gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca 4020
gggacttcag caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg 4080
agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg 4140
cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc 4200
gcagacggac gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga 4260
ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg 4320
tggcacggcg gatgtcggcc gggcgtcgtt ctgggtccat tgttcttctt tactctttgt 4380
gtgactgagg tttggtctag tgctttggtc atctatatat aatgataaca acaatgagaa 4440
caagctttgg agtgatcgga gggtctagga tacatgagat tcaagtggac taggatctac 4500
accgttggat tttgagtgtg gatatgtgtg aggttaattt tacttggtaa cggccacaaa 4560
ggcctaagga gaggtgttga gacccttatc ggcttgaacc gctggaataa tgccacgtgg 4620
aagataattc catgaatctt atcgttatct atgagtgaaa ttgtgtgatg gtggagtggt 4680
gcttgctcat tttacttgcc tggtggactt ggccctttcc ttatggggaa tttatatttt 4740
acttactata gagctttcat accttttttt taccttggat ttagttaata tataatggta 4800
tgattcatga ataaaaatgg gaaatttttg aatttgtact gctaaatgca taagattagg 4860
tgaaactgtg gaatatatat ttttttcatt taaaagcaaa atttgccttt tactagaatt 4920
ataaatatag aaaaatatat aacattcaaa taaaaatgaa aataagaact ttcaaaaaac 4980
agaactatgt ttaatgtgta aagattagtc gcacatcaag tcatctgtta caatatgtta 5040
caacaagtca taagcccaac aaagttagca cgtctaaata aactaaagag tccacgaaaa 5100
tattacaaat cataagccca acaaagttat tgatcaaaaa aaaaaaacgc ccaacaaagc 5160
taaacaaagt ccaaaaaaaa cttctcaagt ctccatcttc ctttatgaac attgaaaact 5220
atacacaaaa caagtcagat aaatctcttt ctgggcctgt cttcccaacc tcctacatca 5280
cttccctatc ggattgaatg ttttacttgt accttttccg ttgcaatgat attgatagta 5340
tgtttgtgaa aactaatagg gttaacaatc gaagtcatgg aatatggatt tggtccaaga 5400
ttttccgaga gctttctagt agaaagccca tcaccagaaa tttactagta aaataaatca 5460
ccaattaggt ttcttattat gtgccaaatt caatataatt atagaggata tttcaaatga 5520
aaacgtatga atgttattag taaatggtca ggtaagacat taaaaaaatc ctacgtcaga 5580
tattcaactt taaaaattcg atcagtgtgg aattgtacaa aaatttggga tctactatat 5640
atatataatg ctttacaaca cttggatttt tttttggagg ctggaatttt taatctacat 5700
atttgttttg gccatgcacc aactcattgt ttagtgtaat actttgattt tgtcaaatat 5760
atgtgttcgt gtatatttgt ataagaattt ctttgaccat atacacacac acatatatat 5820
atatatatat atattatata tcatgcactt ttaattgaaa aaataatata tatatatata 5880
gtgcattttt tctaacaacc atatatgttg cgattgatct gcaaaaatac tgctagagta 5940
atgaaaaata taatctattg ctgaaattat ctcagatgtt aagattttct taaagtaaat 6000
tctttcaaat tttagctaaa agtcttgtaa taactaaaga ataatacaca atctcgacca 6060
cggaaaaaaa acacataata aatttgaatt tcgaccgcgg tacccggaat tgggttataa 6120
ttacctcagg tcgaggaatt aattcggtac gtacctaata acttcgtata gcatacatta 6180
tacgaagtta tatggatctc gaggcattac ggcattacgg cactcgcgag ggtcccaatt 6240
cgagcatgga gccatttaca attgaatata tcctgccgcc gctgccgctt tgcacccggt 6300
ggagcttgca tgttggtttc tacgcagaac tgagccggtt aggcagataa tttccattga 6360
gaactgagcc atgtgcacct tccccccaac acggtgagcg acggggcaac ggagtgatcc 6420
acatgggact tttaaacatc atccgtcgga tggcgttgcg agagaagcag tcgatccgtg 6480
agatcagccg acgcaccggg caggcgcgca acacgatcgc aaagtatttg aacgcaggta 6540
caatcgagcc gacgttcacg gtaccggaac gaccaagcaa gctagcttag taaagccctc 6600
gctagatttt aatgcggatg ttgcgattac ttcgccaact attgcgataa caagaaaaag 6660
ccagcctttc atgatatatc tcccaatttg tgtagggctt attatgcacg cttaaaaata 6720
ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta gtgcatctaa 6780
cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaattgtt agacattatt 6840
tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg 6900
cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga 6960
cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg 7020
cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct 7080
catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa 7140
atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa 7200
cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct 7260
cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag 7320
ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga 7380
gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc 7440
gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct 7500
tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat 7560
ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt 7620
ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc 7680
tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca 7740
tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac 7800
caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt 7860
acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg 7920
gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg 7980
cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct 8040
tctacggcaa gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc 8100
gtccgggcgc ttgccggtgg tgctgacccc ggatgaagtc tctagagctc tagagggttc 8160
gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccagcttctg tatggaacgg 8220
gcatgcggat cagtgagggt ttgcaactgc gggtcaagga tctggatttc gatcacggca 8280
cgatcatcgt gcgggagggc aagggctcca aggatcgggc cttgatgtta cccgagagct 8340
tggcacccag cctgcgcgag cagggatcga tccaacccct ccgctgctat agtgcagtcg 8400
gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta 8460
cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca 8520
taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg 8580
ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg 8640
ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc 8700
gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag 8760
tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca 8820
tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc 8880
cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa 8940
tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc 9000
cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag 9060
gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg 9120
cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg 9180
aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac 9240
aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga 9300
ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg 9360
gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt 9420
ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag 9480
cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg 9540
gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc 9600
gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc 9660
gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt 9720
gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc 9780
gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc 9840
gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg 9900
gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc 9960
gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg 10020
tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc 10080
gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag 10140
gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga 10200
gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa 10260
cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg 10320
aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc 10380
tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga 10440
attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg 10500
gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc 10560
cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc 10620
atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc 10680
cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca 10740
agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc 10800
gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga 10860
cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg 10920
tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc 10980
cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc 11040
ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt 11100
gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca 11160
gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg 11220
tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag 11280
ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc 11340
tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc 11400
cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc 11460
cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc 11520
cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc 11580
ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg 11640
caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca 11700
aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat 11760
tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta 11820
cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc 11880
cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact 11940
gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc 12000
cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg 12060
cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg 12120
gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 12180
acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 12240
cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 12300
gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 12360
agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 12420
gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 12480
ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 12540
aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 12600
ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 12660
gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 12720
cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 12780
gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 12840
tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 12900
cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 12960
cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 13020
gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 13080
agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 13140
cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 13200
tccggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata 13260
gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgcc 13305
<210>34
<211>13326
<212>DNA
<213>人工
<220>
<223>pTVE503
<400>34
agattcgaag ctcggtcccg tgggtgttct gtcgtctcgt tgtacaacga aatccattcc 60
cattccgcgc tcaagatggc ttcccctcgg cagttcatca gggctaaatc aatctagccg 120
acttgtccgg tgaaatgggc tgcactccaa cagaaacaat caaacaaaca tacacagcga 180
cttattcaca cgcgacaaat tacaacggta tatatcctgc cagtactcgg ccgtcgaccg 240
cggtaccccg gaattaagct tgcatgcctg caggcaattg gccgctgtac catgcatgat 300
ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt 360
tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat 420
aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aaatagagag 480
agatagattt gtagagagag actggtgatt tcagcgtgtc caagcttgct agcctaatca 540
atagacataa tgtcaagcgt tgagccttta tgtgcttcac actgctcgac aacttcatca 600
atttttcttg aagcccatgg aaatcttggg ttgattaaga aaggacgtaa gtcaaatctg 660
ttgtcttctg gtgaatactg ttcagcatga taactaggag ttaaaacagt ttgcttgtgt 720
ctgttgatgg catagaagaa gaaaaatctt ttcacctttt cagatatttg acgaggtgtt 780
aactttgggg accattgatg aagaagtttt aagaacatag aataaggacc acatttttca 840
acctttctta ggtaaccaaa cacgcccaat tcttcatacg tcatccccat atctatctca 900
tccgattgaa cgtaatcttt agtcataggt tctaattctg cagttggtgt agcgtttaaa 960
aagtcattca agattggcat gttatattgt tttgatgcgt aggcaatgaa tcttttcaag 1020
tcagtttttg aaataccccc aatagggttg atatctgcgg aggagcagtc atattttgtt 1080
agatacccac gtaagcactc atcaacattt gcgctaccaa gtactaacaa tccacccgag 1140
tttgggatac cacgaaccca cggcaacagt tgcgcaaaaa gataagaaag aaccattctt 1200
agacgcgcct ggatgttttg taaagccaag ttctcgattt gagatccccc aaatattttg 1260
tatattggtt ttttgccagt ggctacttcg aataaggaca ccacactgga taccaatgag 1320
tccatcttta aatccacgtg gtaagatcca attgcattgg aaaggtcctt tgctctgttt 1380
cttgtctcct tggatgaatt ttccgtaccc atgaaacagg agtgaaatat ttttgaggct 1440
agatcctgtg gactgtctgg aatccaatca tcgccgctac gtgttatctt acgaacgtct 1500
ttgataactt gctcatttcc attttgagca gcgtcggtca ctaaacggca catagagtgg 1560
acaatcattg cagttgcaca agagtcaatg cccccagata agggaaggaa aaaccctgtt 1620
ccgttacaac gtcttaaata atcccacatc cagcaagcag gtcccagtgc aatttcttcc 1680
tcaggagagt gatagaaagg ctcgcggact tttgttggac acactgtagg atcaaatctg 1740
gaggtcatta aagccaattc tacaggaata tcaatacgct tgaactttat ttctgccaag 1800
gaggcttgta ggccacgaga catgacagct gcacgataac tcctcacctc ttctaggtcc 1860
acagtagcag taactacttc cacatcatct agcgaaaatt gtgaaccttg ggctacaatt 1920
gtaccattga tggcaattag tgcacagcca tcataatata atctgtcacc atcacaacct 1980
ctttgatttg catacaagta aacaccacca caacgtttag tggcatttaa aattaggtct 2040
aaccttttat ttaacttacg cagttcatga tgagaaccag atgagtttgt catgatttcc 2100
acaccatcta aagacatggc gatgtggggg gattgaggtg taaacaattc ttcacaagtt 2160
tctgtaccaa tgcatgtatc caatgaattt atcacagcgt ccccaaatgg cacaagtctc 2220
tggccggtaa ctttctgaat ctcaggtgga aggataaagt cctccaccac gccaggtttc 2280
atccaaggtg tgaaaaatct catttcccta tagttaccat cattagctaa ccaaatctta 2340
ggtcttatga acaatatctc accatccaag gataacaaac gacaattata acgaacattc 2400
ttgtgtagaa cgggcatacc aatgtcaagt attaatccat gggtttcttt attcttaatg 2460
atttgagcat acatttccca tgaatgaagg caaacgtcat tttctaaaaa atgatctaaa 2520
catccgtagc cagttatttc cagttctggg ccgacacgta acctggcacc cctctctttg 2580
gcaatcttaa tggactgtag gatacggtct ctattacctt caaaatctag ggcccattga 2640
ttcaagttgc atgtagctaa agtgataaga accttgcgct tcttcttggg atgtgccatg 2700
gttttggttt aataagaaga gaaaagagtt cttttgttat ggctgaagta atagagaaat 2760
gagctcgagt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag 2820
gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct 2880
ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat 2940
ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat 3000
ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg 3060
ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc 3120
tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca 3180
ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa aagactctgt atgaactgtt 3240
cgccagtctt cacggcgagt tctgttagat cctcgatctg aatttttgac tccatgtatg 3300
gtgcatatgg cgcgccatat gcccgggccc tgtacagcgg ccgcgttaac gcgtatactc 3360
tagagcgatc gcccgggccg gccatttaaa tgaattcgag ctcggtaccc aaacgcggcc 3420
gcaagctata acttcgtata gcatacatta tacgaagtta ttcgactcta gaggatccca 3480
attcccatgc atggagtcaa agattcaaat agaggacact tctcgaactc ggccgtcgaa 3540
ctcggccgtc gagtacatgg tcgataagaa aaggcaattt gtagatgtta attcccatct 3600
tgaaagaaat atagtttaaa tatttattga taaaataaca agtcaggtat tatagtccaa 3660
gcaaaaacat aaatttattg atgcaagttt aaattcagaa atatttcaat aactgattat 3720
atcagctggt acattgccgt agatgaaaga ctgagtgcga tattatgtgt aatacataaa 3780
ttgatgatat agctagctta gctcatcggg ggatcctaga cgcgtgagat cagatctcgg 3840
tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca 3900
cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc 3960
cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc 4020
tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc 4080
ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt 4140
tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga 4200
cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct 4260
gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg atggtgcaga 4320
ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggtcca 4380
ttgttcttct ttactctttg tgtgactgag gtttggtcta gtgctttggt catctatata 4440
taatgataac aacaatgaga acaagctttg gagtgatcgg agggtctagg atacatgaga 4500
ttcaagtgga ctaggatcta caccgttgga ttttgagtgt ggatatgtgt gaggttaatt 4560
ttacttggta acggccacaa aggcctaagg agaggtgttg agacccttat cggcttgaac 4620
cgctggaata atgccacgtg gaagataatt ccatgaatct tatcgttatc tatgagtgaa 4680
attgtgtgat ggtggagtgg tgcttgctca ttttacttgc ctggtggact tggccctttc 4740
cttatgggga atttatattt tacttactat agagctttca tacctttttt ttaccttgga 4800
tttagttaat atataatggt atgattcatg aataaaaatg ggaaattttt gaatttgtac 4860
tgctaaatgc ataagattag gtgaaactgt ggaatatata tttttttcat ttaaaagcaa 4920
aatttgcctt ttactagaat tataaatata gaaaaatata taacattcaa ataaaaatga 4980
aaataagaac tttcaaaaaa cagaactatg tttaatgtgt aaagattagt cgcacatcaa 5040
gtcatctgtt acaatatgtt acaacaagtc ataagcccaa caaagttagc acgtctaaat 5100
aaactaaaga gtccacgaaa atattacaaa tcataagccc aacaaagtta ttgatcaaaa 5160
aaaaaaaacg cccaacaaag ctaaacaaag tccaaaaaaa acttctcaag tctccatctt 5220
cctttatgaa cattgaaaac tatacacaaa acaagtcaga taaatctctt tctgggcctg 5280
tcttcccaac ctcctacatc acttccctat cggattgaat gttttacttg taccttttcc 5340
gttgcaatga tattgatagt atgtttgtga aaactaatag ggttaacaat cgaagtcatg 5400
gaatatggat ttggtccaag attttccgag agctttctag tagaaagccc atcaccagaa 5460
atttactagt aaaataaatc accaattagg tttcttatta tgtgccaaat tcaatataat 5520
tatagaggat atttcaaatg aaaacgtatg aatgttatta gtaaatggtc aggtaagaca 5580
ttaaaaaaat cctacgtcag atattcaact ttaaaaattc gatcagtgtg gaattgtaca 5640
aaaatttggg atctactata tatatataat gctttacaac acttggattt ttttttggag 5700
gctggaattt ttaatctaca tatttgtttt ggccatgcac caactcattg tttagtgtaa 5760
tactttgatt ttgtcaaata tatgtgttcg tgtatatttg tataagaatt tctttgacca 5820
tatacacaca cacatatata tatatatata tatattatat atcatgcact tttaattgaa 5880
aaaataatat atatatatat agtgcatttt ttctaacaac catatatgtt gcgattgatc 5940
tgcaaaaata ctgctagagt aatgaaaaat ataatctatt gctgaaatta tctcagatgt 6000
taagattttc ttaaagtaaa ttctttcaaa ttttagctaa aagtcttgta ataactaaag 6060
aataatacac aatctcgacc acggaaaaaa aacacataat aaatttgaat ttcgaccgcg 6120
gtacccggaa ttgggttata attacctcag gtcgaggaat taattcggta cgtacctaat 6180
aacttcgtat agcatacatt atacgaagtt atatggatct cgaggcatta cggcattacg 6240
gcactcgcga gggtcccaat tcgagcatgg agccatttac aattgaatat atcctgccgc 6300
cgctgccgct ttgcacccgg tggagcttgc atgttggttt ctacgcagaa ctgagccggt 6360
taggcagata atttccattg agaactgagc catgtgcacc ttccccccaa cacggtgagc 6420
gacggggcaa cggagtgatc cacatgggac ttttaaacat catccgtcgg atggcgttgc 6480
gagagaagca gtcgatccgt gagatcagcc gacgcaccgg gcaggcgcgc aacacgatcg 6540
caaagtattt gaacgcaggt acaatcgagc cgacgttcac ggtaccggaa cgaccaagca 6600
agctagctta gtaaagccct cgctagattt taatgcggat gttgcgatta cttcgccaac 6660
tattgcgata acaagaaaaa gccagccttt catgatatat ctcccaattt gtgtagggct 6720
tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa 6780
ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 6840
aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga 6900
caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag 6960
cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt 7020
cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca 7080
acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta 7140
aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg 7200
ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat 7260
caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc 7320
caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa 7380
tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa 7440
ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca 7500
aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg 7560
ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg 7620
atacttcggc gatcaccgct tccctcatga tgtttaactt tgttttaggg cgactgccct 7680
gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt 7740
gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga 7800
aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 7860
agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc 7920
gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 7980
aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 8040
cattggcggc cttgctgttc ttctacggca agtgctgtgc acggatctgc cctggcttca 8100
ggagatcgga agacctcggc cgtccgggcg cttgccggtg gtgctgaccc cggatgaagt 8160
ctctagagct ctagagggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg 8220
cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg 8280
atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg 8340
ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagggatcg atccaacccc 8400
tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca 8460
tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc 8520
ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg 8580
ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc 8640
aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg 8700
agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac 8760
gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc 8820
tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc 8880
cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg 8940
ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg 9000
cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc 9060
gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc 9120
atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca 9180
ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg 9240
agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt 9300
tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc 9360
cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct 9420
ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg 9480
atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt 9540
aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc 9600
gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc 9660
gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg 9720
gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag 9780
gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct 9840
gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac 9900
atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga 9960
aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag 10020
gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc 10080
gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc 10140
gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt 10200
aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg 10260
cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg 10320
tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag 10380
gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca 10440
aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa 10500
caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc 10560
gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa 10620
tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 10680
cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 10740
acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 10800
gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 10860
tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 10920
cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 10980
agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 11040
cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 11100
gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 11160
gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 11220
cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 11280
cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 11340
gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 11400
ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 11460
ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 11520
ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 11580
gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 11640
gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 11700
tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt 11760
tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 11820
cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 11880
agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 11940
ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc 12000
tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 12060
tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 12120
gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 12180
atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 12240
cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 12300
gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 12360
atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 12420
aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 12480
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 12540
agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 12600
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 12660
caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 12720
gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 12780
cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 12840
tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 12900
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 12960
cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 13020
tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 13080
tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 13140
caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 13200
aaaaaaagga tctcaagaag atccggaaaa cgcaagcgca aagagaaagc aggtagcttg 13260
cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 13320
attgcc 13326
Claims (11)
1.获得具有增加的胁迫抗性的植物的方法，其包括
a.向植物细胞中引入嵌合基因获得转基因细胞，所述嵌合基因含有以下有效连接的DNA片段：
i.可在植物中表达的启动子；
ii.编码来自酵母或真菌的烟酰胺腺嘌呤二核苷酸补救合成途径中的酶的DNA区，其中所述DNA区编码含有选自SEQ ID No.：2或SEQ ID No.：4的氨基酸序列的酶；
iii.参与转录终止和多聚腺苷酸化作用的3’末端区；
b.再生所述的转基因细胞以获得转基因植物群；和
c.从所述转基因植物群中选择表现为增加的胁迫抗性的植物或选择在胁迫条件下与类似的非转基因植物相比表现为降低的活性氧水平或维持高水平NADH的植物。
2.根据权利要求1的方法，其中所述DNA区含有SEQ IDNo.：1或SEQ ID No.：3的核苷酸序列。
3.根据权利要求1或2的方法，所述方法还包括将所述植物与其他植物杂交的步骤。
4.权利要求1或2任一项中描述的嵌合基因。
5.含有权利要求4所述嵌合基因的植物细胞。
6.权利要求5的植物细胞，其为芸苔属植物的植物细胞。
7.权利要求5的植物细胞，其选自棉花、油料种子油菜、小麦、玉米、大麦、向日葵、稻、燕麦、甘蔗、大豆、菊苣、莴苣、番茄、烟草、马铃薯、甜菜、木瓜、菠萝、芒果或拟南芥植物细胞。
8.权利要求5的植物细胞，其为蔬菜植物细胞。
9.根据权利要求5或6或7或8所述的植物细胞，其特征还在于在胁迫条件下与不含这类嵌合基因的类似植物细胞相比，其活性氧水平更低。
10.根据权利要求4的嵌合基因用于增加植物胁迫抗性的用途。
11.根据权利要求4的嵌合基因的用途，所述用途为在胁迫条件下降低植物或植物细胞中活性氧水平或在胁迫条件下维持植物或植物细胞中NAD水平。
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04077624.7 | 2004-09-24 | ||
| EP04077624 | 2004-09-24 | ||
| US62882604P | 2004-11-17 | 2004-11-17 | |
| US60/628,826 | 2004-11-17 | ||
| PCT/EP2005/010168 WO2006032469A2 (en) | 2004-09-24 | 2005-09-16 | Stress resistant plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101040049A CN101040049A (zh) | 2007-09-19 |
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Families Citing this family (90)
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| US7977049B2 (en) | 2002-08-09 | 2011-07-12 | President And Fellows Of Harvard College | Methods and compositions for extending the life span and increasing the stress resistance of cells and organisms |
| WO2006045633A1 (en) | 2004-10-29 | 2006-05-04 | Bayer Bioscience N.V. | Stress tolerant cotton plants |
| WO2006105440A2 (en) * | 2005-03-30 | 2006-10-05 | Sirtris Pharmaceuticals, Inc. | Nicotinamide riboside and analogues thereof |
| MX2007016199A (es) * | 2005-06-15 | 2008-03-11 | Bayer Bioscience Nv | Metodos para aumentar la resistencia de plantas a condiciones hipoxicas. |
| AU2007229005B2 (en) * | 2006-03-21 | 2012-05-31 | Bayer Cropscience Nv | Stress resistant plants |
| CL2007003743A1 (es) * | 2006-12-22 | 2008-07-11 | Bayer Cropscience Ag | Composicion que comprende fenamidona y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos. |
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| EP1969930A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | Phenoxyphenylamidine und deren Verwendung als Fungizide |
| EP1969934A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | 4-Cycloalkyl-oder 4-arylsubstituierte Phenoxyphenylamidine und deren Verwendung als Fungizide |
| EP2136627B1 (de) | 2007-03-12 | 2015-05-13 | Bayer Intellectual Property GmbH | Dihalogenphenoxyphenylamidine und deren verwendung als fungizide |
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| DE102007045956A1 (de) * | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Wirkstoffkombination mit insektiziden und akariziden Eigenschaften |
| CA2701290A1 (en) * | 2007-10-02 | 2009-04-16 | Bayer Cropscience Ag | Methods of improving plant growth |
| JP5570731B2 (ja) * | 2008-02-28 | 2014-08-13 | 旭化成ファーマ株式会社 | ピロリン酸の測定方法 |
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| EP2662361A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazol indanyl carboxamides |
| WO2013167544A1 (en) | 2012-05-09 | 2013-11-14 | Bayer Cropscience Ag | 5-halogenopyrazole indanyl carboxamides |
| EP2735231A1 (en) | 2012-11-23 | 2014-05-28 | Bayer CropScience AG | Active compound combinations |
| CN104837351A (zh) | 2012-11-30 | 2015-08-12 | 拜耳作物科学股份公司 | 二元杀真菌或杀虫混合物 |
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| CA2892693C (en) | 2012-11-30 | 2021-08-10 | Bayer Cropscience Ag | Binary fungicidal mixtures |
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| AR093996A1 (es) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | Combinaciones bactericidas y fungicidas binarias |
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| US20160016944A1 (en) | 2013-03-07 | 2016-01-21 | Bayer Cropscience Aktiengesellschaft | Fungicidal 3--heterocycle derivatives |
| JP2016522800A (ja) | 2013-04-12 | 2016-08-04 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | 新規トリアゾリンチオン誘導体 |
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| WO2014170364A1 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Ag | Binary insecticidal or pesticidal mixture |
| CN105555135B (zh) | 2013-04-19 | 2018-06-15 | 拜耳作物科学股份公司 | 涉及邻苯二甲酰胺衍生物应用的用于改善对转基因植物生产潜能的利用的方法 |
| WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
| TW201507722A (zh) | 2013-04-30 | 2015-03-01 | Bayer Cropscience Ag | 做為殺線蟲劑及殺體內寄生蟲劑的ｎ－（２－鹵素－２－苯乙基）－羧醯胺類 |
| EP3041338B1 (en) | 2013-09-04 | 2019-12-11 | Indigo AG, Inc. | Agricultural endophyte-plant compositions, and methods of use |
| EP3436575A1 (en) | 2015-06-18 | 2019-02-06 | The Broad Institute Inc. | Novel crispr enzymes and systems |
| WO2017174430A1 (en) | 2016-04-06 | 2017-10-12 | Bayer Cropscience Aktiengesellschaft | Combination of nuclear polyhedrosis virus and diamides |
| CA3032030A1 (en) | 2016-07-29 | 2018-02-01 | Bayer Cropscience Aktiengesellschaft | Active compound combinations and methods to protect the propagation material of plants |
| US20190281828A1 (en) | 2016-09-22 | 2019-09-19 | Bayer Cropscience Aktiengesellschaft | Novel triazole derivatives |
| EP3515907A1 (en) | 2016-09-22 | 2019-07-31 | Bayer CropScience Aktiengesellschaft | Novel triazole derivatives |
| US20210323950A1 (en) | 2018-06-04 | 2021-10-21 | Bayer Aktiengesellschaft | Herbicidally active bicyclic benzoylpyrazoles |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1360466A (zh) * | 1999-07-05 | 2002-07-24 | 科学技术振兴事业团 | 耐缺铁性稻类的构建 |
| WO2004016726A2 (en) * | 2002-08-09 | 2004-02-26 | President And Fellows Of Harvard College | Methods and compositions for extending the life span and increasing the stress resistance of cells and organisms |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2811889A (en) | 1987-10-20 | 1989-05-23 | Plant Genetic Systems N.V. | A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants |
| GB8810120D0 (en) | 1988-04-28 | 1988-06-02 | Plant Genetic Systems Nv | Transgenic nuclear male sterile plants |
| US5639948A (en) | 1991-02-08 | 1997-06-17 | Plant Genetic Systems, N.V. | Stamen-specific promoters from rice |
| US5792921A (en) * | 1992-02-14 | 1998-08-11 | Londesborough; John | Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase |
| US6211431B1 (en) | 1994-08-30 | 2001-04-03 | Commonwealth Scientific And Industrial Research Organization | Plant transcription regulators from circovirus |
| AU718082B2 (en) | 1995-10-06 | 2000-04-06 | Plant Genetic Systems N.V. | Seed shattering |
| US6693185B2 (en) | 1998-07-17 | 2004-02-17 | Bayer Bioscience N.V. | Methods and means to modulate programmed cell death in eukaryotic cells |
| US7314974B2 (en) * | 2002-02-21 | 2008-01-01 | Monsanto Technology, Llc | Expression of microbial proteins in plants for production of plants with improved properties |
| US7977049B2 (en) * | 2002-08-09 | 2011-07-12 | President And Fellows Of Harvard College | Methods and compositions for extending the life span and increasing the stress resistance of cells and organisms |
| JP2004261136A (ja) * | 2003-03-04 | 2004-09-24 | Univ Tokyo | Ｎａｄ代謝活性化によるストレス耐性植物 |
| JP4960699B2 (ja) | 2003-04-09 | 2012-06-27 | バイエル・バイオサイエンス・エヌ・ヴェー | ストレス条件に対する植物の耐性を増加させるための方法及び手段 |
| AU2007229005B2 (en) * | 2006-03-21 | 2012-05-31 | Bayer Cropscience Nv | Stress resistant plants |
-
2005
- 2005-09-16 SI SI200530927T patent/SI1794306T1/sl unknown
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-
2010
- 2010-03-12 CY CY20101100240T patent/CY1109892T1/el unknown
- 2010-10-29 US US12/916,180 patent/US8802927B2/en not_active Expired - Fee Related
-
2014
- 2014-08-11 US US14/456,488 patent/US9534230B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1360466A (zh) * | 1999-07-05 | 2002-07-24 | 科学技术振兴事业团 | 耐缺铁性稻类的构建 |
| WO2004016726A2 (en) * | 2002-08-09 | 2004-02-26 | President And Fellows Of Harvard College | Methods and compositions for extending the life span and increasing the stress resistance of cells and organisms |
Non-Patent Citations (2)
| Title |
|---|
| 顾采琴，等，.草莓和番茄果实采后NAD激酶、NADP磷酸酶活性变化的研究.中国农业科学35 11.2002,35(11),1381-1384. |
| 顾采琴,等,.草莓和番茄果实采后NAD激酶、NADP磷酸酶活性变化的研究.中国农业科学35 11.2002,35(11),1381-1384. * |
Also Published As
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| ATE452199T1 (de) | 2010-01-15 |
| BRPI0517331A (pt) | 2008-10-07 |
| CA2581257A1 (en) | 2006-03-30 |
| US9534230B2 (en) | 2017-01-03 |
| KR20120138840A (ko) | 2012-12-26 |
| US7851675B2 (en) | 2010-12-14 |
| EP1794306B1 (en) | 2009-12-16 |
| AU2005287499A1 (en) | 2006-03-30 |
| SI1794306T1 (sl) | 2010-04-30 |
| KR20070060128A (ko) | 2007-06-12 |
| EP1794306A2 (en) | 2007-06-13 |
| AR056256A1 (es) | 2007-10-03 |
| AU2005287499B2 (en) | 2010-09-23 |
| KR101331677B1 (ko) | 2013-11-26 |
| CA2581257C (en) | 2014-04-15 |
| WO2006032469A3 (en) | 2006-07-06 |
| US20140366216A1 (en) | 2014-12-11 |
| ZA200701725B (en) | 2008-06-25 |
| EP2175026B1 (en) | 2014-07-30 |
| US20110131672A1 (en) | 2011-06-02 |
| US20070300322A1 (en) | 2007-12-27 |
| PL1794306T3 (pl) | 2010-05-31 |
| CN101040049A (zh) | 2007-09-19 |
| WO2006032469A2 (en) | 2006-03-30 |
| DK1794306T3 (da) | 2010-04-12 |
| DE602005018379D1 (de) | 2010-01-28 |
| EP2175026A1 (en) | 2010-04-14 |
| PT1794306E (pt) | 2010-03-22 |
| CY1109892T1 (el) | 2014-09-10 |
| US8802927B2 (en) | 2014-08-12 |
| ES2338443T3 (es) | 2010-05-07 |
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